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40 results on '"Lahaie, Isabelle"'

7. Tyrosine-Protein Phosphatase Non-receptor Type 9 (PTPN9) Negatively Regulates the Paracrine Vasoprotective Activity of Bone-Marrow Derived Pro-angiogenic Cells: Impact on Vascular Degeneration in Oxygen-Induced Retinopathy

Author

Desjarlais, Michel, primary, Ruknudin, Pakiza, additional, Wirth, Maëlle, additional, Lahaie, Isabelle, additional, Dabouz, Rabah, additional, Rivera, José Carlos, additional, Habelrih, Tiffany, additional, Omri, Samy, additional, Hardy, Pierre, additional, Rivard, Alain, additional, and Chemtob, Sylvain, additional

Published
2021
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9. Antenatal antioxidant prevents adult hypertension, vascular dysfunction, and microvascular rarefaction associated with in utero exposure to a low-protein diet

Author

Cambonie, Gilles, Comte, Blandine, Yzydorczyk, Catherine, Ntimbane, Thierry, Germain, Nathalie, Le, Ngoc Loan Oanh, Pladys, Patrick, Gauthier, Cindy, Lahaie, Isabelle, Abran, Daniel, Lavoie, Jean-Claude, and Nuyt, Anne Monique

Subjects
Oxidative stress -- Health aspects, Antioxidants -- Health aspects, Hypertension -- Research, Biological sciences
Abstract

Developmental programming of hypertension is associated with vascular dysfunction characterized by impaired vasodilatation to nitric oxide, exaggerated vaso-constriction to ANG II, and microvascular rarefaction appearing in the neonatal period. Hypertensive adults have indices of increased oxidative stress, and newborns that were nutrient depleted during fetal life have decreased antioxidant defenses and increased susceptibility to oxidant injury. To test the hypothesis that oxidative stress participates in early life programming of hypertension, vascular dysfunction, and microvascular rarefaction associated with maternal protein deprivation, pregnant rats were fed a normal, low protein (LP), or LP plus lazaroid (lipid peroxidation inhibitor) isocaloric diet from the day of conception until delivery. Lazaroid administered along with the LP diet prevented blood pressure elevation, enhanced vasomotor response to ANG II, impaired vasodilatation to sodium nitroprusside, and microvascular rarefaction in adult offspring. Liver total glutathione was significantly decreased in LP fetuses, and kidney eight-isoprostaglandin F2[alpha] (8-iso[PGF.sub.2[alpha]]) levels were significantly increased in adult LP offspring; these modifications were prevented by lazaroid. Renal nitrotyrosine abundance and blood levels of 1,4-dihydroxynonene and 4-hydroxynonenal-protein adducts were not modified by antenatal diet exposure. This study shows in adult offspring of LP-fed dams prevention of hypertension, vascular dysfunction, microvascular rarefaction, and of an increase in indices of oxidative stress by the administration of lazaroid during gestation. Lazaroid also prevented the decrease in antioxidant glutathione levels in fetuses, suggesting an antenatal mild oxidative stress in offspring of LP-fed dams. These studies support the concept that perinatal oxidative insult can lead to permanent alterations in the cardiovascular system development. hypertension; vascular dysfunction; developmental origin of adult disease; oxidative stress; antioxidants.

Published
2007

15. Propranolol Attenuates Proangiogenic Activity of Mononuclear Phagocytes: Implication in Choroidal Neovascularization

Author

Omri, Samy, primary, Tahiri, Houda, additional, Pierre, Wyston Chadwick, additional, Desjarlais, Michel, additional, Lahaie, Isabelle, additional, Loiselle, Sarah-Eve, additional, Rezende, Flavio, additional, Lodygensky, Gregory, additional, Hebert, Terence E., additional, Ong, Huy, additional, and Chemtob, Sylvain, additional

Published
2019
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16. A novel mechanism for vasoconstrictor action of 8-isoprostaglandin F2-alpha on retinal vessels

Author

Lahaie, Isabelle, Hardy, Pierre, Hou, Xin, Hassessian, Haroutioun, Asselin, Pierre, Lachapelle, Pierre, Almazan, Guillermina, Varma, Daya R., Morrow, Jason D., Roberts, L. Jackson, II, and Chemtob, Sylvain

Subjects
Rats -- Physiological aspects, Vasoconstriction -- Research, Prostaglandins -- Physiological aspects, Retina -- Blood-vessels, Retinal diseases -- Causes of, Biological sciences
Abstract

Halothane-anesthetized one-to-three-day-old piglets were studied to examine the influence of 8-isoprostaglandin F2-alpha (8-iso-PGF2-alpha) on the retinal vessels of the piglets. The retinal vasoconstriction caused by the 8-iso-PGF2-alpha was found to be mediated by the endothelin following the introduction of Ca2+ into the cells and the cyclooxygenase-produced formation of thromboxane. Retinal vasoconstriction to 8-isoprostanes may thus contribute in the production of ischemic retinopathies.

Published
1998

19. Additional file 5: Figure S5. of Tetrahydrobiopterin (BH4) deficiency is associated with augmented inflammation and microvascular degeneration in the retina

Author

Rivera, José, Baraa Noueihed, Ankush Madaan, Lahaie, Isabelle, Jingyi Pan, Jaques Belik, and Chemtob, Sylvain

Subjects
sense organs, eye diseases
Abstract

Tetrahydrobiopterin (BH4) deficiency is associated with upregulated expression of pro-inflammatory mediators at P14. Quantitative real-time PCR analysis was performed on whole retinas at P7 and P14 from control (WT) and hph-1 animals; control values were set at 1. A significant increase in retinal mRNA expression of IL-1β (p

Published
2017
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20. Additional file 7: Figure S7. of Tetrahydrobiopterin (BH4) deficiency is associated with augmented inflammation and microvascular degeneration in the retina

Author

Rivera, José, Baraa Noueihed, Ankush Madaan, Lahaie, Isabelle, Jingyi Pan, Jaques Belik, and Chemtob, Sylvain

Subjects
sense organs, eye diseases
Abstract

Intraocular supplementation of tetrahydrobiopterin (BH4) prevents retinal vasoobliteration in mice exposed to oxygen-induced retinopathy (OIR). Representative images of flat-mounted retinas labeled with fluorescein-labeled Griffonia Simplicifolia Lectin 1 (GSL 1), isolectin B4 to examine vasoobliteration in animals exposed to 75% oxygen from P7 until P9. Animals were intravitreally injected at P7 with 100 μM of BH4 or vehicle (PBS steril 1×) and retinas analyzed at P9. Significant differences (p value) in the vasoobliterated area between vehicle and BH4 treatment after 48 h of hyperoxia are shown in the graphs; ***p

Published
2017
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21. Additional file 1: Figure S1. of Tetrahydrobiopterin (BH4) deficiency is associated with augmented inflammation and microvascular degeneration in the retina

Author

JosĂŠ Rivera, Baraa Noueihed, Ankush Madaan, Lahaie, Isabelle, Jingyi Pan, Jaques Belik, and Chemtob, Sylvain

Subjects
genetic structures, sense organs, eye diseases
Abstract

Morphology of the eyes from hph-1 and WT mice. (A) Representative images of the eyeballs from wild type (WT) and hph-1 mice analyzed by H&E staining at postnatal day 1, 7, and 14. The pictures show a reduction in the size, as well as, appearance of hypertrophy in the iris and persistence of fetal hyaloid vasculature in the eyes of hph-1 mice. (PDF 297Â kb)

Published
2017
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22. Additional file 2: Figure S2. of Tetrahydrobiopterin (BH4) deficiency is associated with augmented inflammation and microvascular degeneration in the retina

Author

Rivera, José, Baraa Noueihed, Ankush Madaan, Lahaie, Isabelle, Jingyi Pan, Jaques Belik, and Chemtob, Sylvain

Subjects
sense organs, eye diseases
Abstract

Tetrahydrobiopterin (BH4) content in hph-1 mice at early ages. BH4 content (ng/mg of tissue) in retinas from control (WT) and hph-1 mice at postnatal day 7 and 14 (P7 and P14) was measured by using liquid chromatography tandem mass spectrometry (LC-MS/MS). The levels of BH4 were significantly decreased by ~ 90% in the retinas of hph-1 at P7 and P14 compared with WT mice. Results showed in the histograms are expressed as means ± SEM of n = 5 mice for each group. **p

Published
2017
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23. Additional file 3: Figure S3. of Tetrahydrobiopterin (BH4) deficiency is associated with augmented inflammation and microvascular degeneration in the retina

Author

JosĂŠ Rivera, Baraa Noueihed, Ankush Madaan, Lahaie, Isabelle, Jingyi Pan, Jaques Belik, and Chemtob, Sylvain

Subjects
genetic structures, sense organs, eye diseases
Abstract

Retinal flat-mounts from hph-1 and WT mice at different ages. Representative images of whole-mounted retinas labeled with TRITC-conjugated lectin endothelial cell marker Bandeiraea simplicifolia showing retinal vasculature from controls (WT) and hph-1 mice at different postnatal ages (P1, P7, P14, and P22). Note that at P1, the vessels in the retina are mainly represented by the hyaloid vasculature. (PDF 263Â kb)

Published
2017
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24. Additional file 6: Figure S6. of Tetrahydrobiopterin (BH4) deficiency is associated with augmented inflammation and microvascular degeneration in the retina

Author

Rivera, José, Baraa Noueihed, Ankush Madaan, Lahaie, Isabelle, Jingyi Pan, Jaques Belik, and Chemtob, Sylvain

Subjects
sense organs
Abstract

Interleukin-1β (IL-1β) is localized on microglial cells and Semaphorin 3A (Sema3A) is decreased in the retinas from BH4 deficient mice. (A) Representative confocal images showing immunoreactivity for IL-1β (green) and Iba-1 (red) merged with DAPI (blue and yellow) in retinal cryosections from control (WT) and hph-1 mice at 22-day-old (n = 3 per group). Co-staining of IL-1β with Iba-1 was detected on microglial cells localized in the ganglion cell layer (GCL) and deep plexiform layer (IPL) in retinas from hph-1 mice but not in WT mice. Scale bar = 50 μm. (B) Quantitative real-time PCR analysis was performed on whole retinas at P22 from control (WT) and hph-1 animals; control values were set at 1. A significant decrease in retinal mRNA expression of Sema3A (***p

Published
2017
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25. Additional file 4: Figure S4. of Tetrahydrobiopterin (BH4) deficiency is associated with augmented inflammation and microvascular degeneration in the retina

Author

Rivera, José, Baraa Noueihed, Ankush Madaan, Lahaie, Isabelle, Jingyi Pan, Jaques Belik, and Chemtob, Sylvain

Subjects
sense organs
Abstract

Vascular density in hph-1 and WT mice at early ages. The quantification of the vascular density in both superficial and deep retinal vascular complexes was not significant in hph-1 mice compared to the WT control at P1, P7, and P14. Results showed in the histograms are expressed as means ± SEM of n = 3–4 retinas for each group. ns = not significant compared to the control. (PDF 193 kb)

Published
2017
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29. Erratum: Nuclear localization of platelet-activating factor receptor controls retinal neovascularization

Author

Bhosle, Vikrant K, primary, Rivera, José Carlos, additional, Zhou, Tianwei, additional, Omri, Samy, additional, Sanchez, Melanie, additional, Hamel, David, additional, Zhu, Tang, additional, Rouget, Raphael, additional, Rabea, Areej Al, additional, Hou, Xin, additional, Lahaie, Isabelle, additional, Ribeiro-da-Silva, Alfredo, additional, and Chemtob, Sylvain, additional

Published
2016
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30. Development of a Novel Noncompetitive Antagonist of IL-1 Receptor

Author

Quiniou, Christiane, primary, Sapieha, Przemyslaw, additional, Lahaie, Isabelle, additional, Hou, Xin, additional, Brault, Sonia, additional, Beauchamp, Martin, additional, Leduc, Martin, additional, Rihakova, Lenka, additional, Joyal, Jean-Sébastien, additional, Nadeau, Sylvain, additional, Heveker, Nikolaus, additional, Lubell, William, additional, Sennlaub, Florian, additional, Gobeil, Fernand, additional, Miller, Greg, additional, Pshezhetsky, Alexey V., additional, and Chemtob, Sylvain, additional

Published
2008
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31. Dominant Role for Calpain in Thromboxane-Induced Neuromicrovascular Endothelial Cytotoxicity

Author

Quiniou, Christiane, primary, Sennlaub, Florian, additional, Beauchamp, Martin H., additional, Checchin, Daniella, additional, Lahaie, Isabelle, additional, Brault, Sonia, additional, Gobeil, Fernand, additional, Sirinyan, Mirna, additional, Kooli, Amna, additional, Hardy, Pierre, additional, Pshezhetsky, Alexey, additional, and Chemtob, Sylvain, additional

Published
2005
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35. Cyclooxygenase- (COX) 2 is the main intrinsic source of prostaglandins (PG) in the ductus arteriosus (DA) of the newborn (NB) but not of the fetus. • 109

Author

Guerguerian, Anne-Marie, primary, Hardy, Pierre, additional, Lahaie, Isabelle, additional, Bhattacharya, Mousumi, additional, Peri, Krishna, additional, Segar, Jeffrey, additional, Olley, Peter, additional, Fouron, Jean-Claude, additional, St. Louis, Jean, additional, Varma, Daya R, additional, and Chemtob, Sylvain, additional

Published
1997
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38. A novel mechanism for vasoconstrictor action of 8-isoprostaglandin F2... on retinal vessels.

Author

Lahaie, Isabelle, Hardy, Pierre, Xin Hou, Hasséssian, Haroutioun, Asselin, Pierre, Lachapelle, Pierre, Almazan, Guillermina, Varma, Daya R., Morrow, Jason D., Roberts II, L. Jackson, and Chemtob, Sylvain

Subjects
*PHYSIOLOGY
Abstract

Presents information on a study which described the effects of 8-isoprostaglandin F2alpha on the retinal vasculature from piglets using a video-imaging technique. Role of oxidant stress in the formation of reactive oxygen species; How isoprostanes are produced; Characteristics of 8-isoprostaglandin F2alpha.

Published
1998

39. Dominant role for calpain in thromboxane-induced neuromicrovascular endothelial cytotoxicity.

Author

Quiniou, Christiane, Sennlaub, Florian, Beauchamp, Martin H, Checchin, Daniella, Lahaie, Isabelle, Brault, Sonia, Gobeil, Fernand, Sirinyan, Mirna, Kooli, Amna, Hardy, Pierre, Pshezhetsky, Alexey, and Chemtob, Sylvain

Abstract

Thromboxane A(2) (TXA(2)) is an important lipid mediator generated during oxidative stress and implicated in ischemic neural injury. This autacoid was recently shown to partake in this injury process by directly inducing endothelial cytotoxicity. We explored the mechanisms for this TXA(2)-evoked neural microvascular endothelial cell death. Stable TXA(2) mimetics 5-heptenoic acid, 7-[6-(3-hydroxy-1-octenyl)-2-oxabicyclo[2.2.1]hept-5-yl]-[1R-[1alpha,4alpha,5beta(Z),6alpha,(1E,3S)]]-9,11-dedioxy-9alpha,11alpha-methanolpoxy (U-46619) [as well as [1S-[1alpha,2alpha(Z),3beta(1E,3S(*)),4alpha]]-7-[3-[3-hydroxy-4-(4-iodophenoxy)-1-butenyl]-7-oxabicyclo[2.1.1]-hept-2-yl]-5-heptenoic acid; I-BOP] induced a retinal microvascular degeneration in rat pups in vivo and in porcine retinal explants ex vivo and death of porcine brain endothelial cells (in culture). TXA(2) dependence of these effects was corroborated by antagonism using the selective TXA(2) receptor blocker (-)-6,8-difluoro-9-p-methyl-sulfonyl-benzyl-1,2,3,4-tetrahydrocarbazol-1-yl-acetic acid (L670596). In all cases, neurovascular endothelial cell death was prevented by pan-calpain and specific m-calpain inhibitors but not by caspase-3 or pan-caspase inhibitors. Correspondingly, TXA(2) (mimetics) augmented generation of known active m-calpain (but not mu-calpain) form and increased the activity of m-calpain (cleavage of fluorogenic substrate N-succinyl-Leu-Leu-Val-Tyr-7-amino-4-methylcoumarin; and of alpha-spectrin into specific fragments) but not of pan-caspase or specific caspase-3 (respectively, using sulforhodamine-Val-Arg-Asp-fluoromethyl ketone and detecting its active 17- and 12-kDa fragments). Interestingly, these effects were phospholipase C (PLC)-dependent [associated with increase in inositol triphosphate and inhibited by PLC blocker 1-[6-[[17beta-methoxyestra-1,3,5(10)-trien-17-yl]amino]hexyl]-1H-pyrrole-2,5-dione (U73122)] and required calcium but were not associated with increased intracellular calcium. U-46619-induced calpain activation resulted in translocation of Bax to the mitochondria, loss of polarization of the latter (using potentiometric probe 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyanine iodide; JC-1) and in turn release of cytochrome c into the cytosol and depletion of cellular ATP; these effects were all blocked by calpain inhibitors. Overall, this work identifies (specifically) m-calpain as a dominant protease in TXA(2)-induced neurovascular endothelial cell death.

Published
2006
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40. Nuclear localization of platelet-activating factor receptor controls retinal neovascularization.

Author

Bhosle VK, Rivera JC, Zhou TE, Omri S, Sanchez M, Hamel D, Zhu T, Rouget R, Rabea AA, Hou X, Lahaie I, Ribeiro-da-Silva A, and Chemtob S

Abstract

Platelet-activating factor (PAF) is a pleiotropic phospholipid with proinflammatory, procoagulant and angiogenic actions on the vasculature. We and others have reported the presence of PAF receptor (Ptafr) at intracellular sites such as the nucleus. However, mechanisms of localization and physiologic functions of intracellular Ptafr remain poorly understood. We hereby identify the importance of C-terminal motif of the receptor and uncover novel roles of Rab11a GTPase and importin-5 in nuclear translocation of Ptafr in primary human retinal microvascular endothelial cells. Nuclear localization of Ptafr is independent of exogenous PAF stimulation as well as intracellular PAF biosynthesis. Moreover, nuclear Ptafr is responsible for the upregulation of unique set of growth factors, including vascular endothelial growth factor, in vitro and ex vivo. We further corroborate the intracrine PAF signaling, resulting in angiogenesis in vivo, using Ptafr antagonists with distinct plasma membrane permeability. Collectively, our findings show that nuclear Ptafr translocates in an agonist-independent manner, and distinctive functions of Ptafr based on its cellular localization point to another dimension needed for pharmacologic selectivity of drugs.

Published
2016
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