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Wearable assistive devices are vitally important for tetraplegic individuals to provide valuable insights into their intended directives tailored to tongue motions in wireless healthcare industries. The flexible differentially driven extraoral antenna and rectenna measurement system are developed to enable differential sensing and monitoring of the set of unique tongue gestures for extraoral tongue drive system (eTDS) applications in three frequency bands of Industrial, Scientific, and Medical (ISM) (915.0 MHz, 2400 MHz, and 5800 MHz). The performance analysis is carried out using the heterogeneous human head model. The differential rectifier is coplanarly integrated with the differential extraoral antenna on the same 0.254 mm thin and 9.5 mm wider Rogers RT/ Duroid 6010 LM substrate. The footprint of the fabricated differential rectenna is 0.135 [Formula: see text] 0.082 [Formula: see text][Formula: see text] 0.002 [Formula: see text] where the planar size of differential rectifier is 15.75[Formula: see text] 2.5 mm 2 . The fabricated systems are situated closely to an artificial head model. The maximum conversion efficiency achieved at 2400 MHz and 5800 MHz is 83.45% and 74.8%, respectively, for 11 dBm of RF input power. Further, the link analysis, including interfacing circuit losses, was carried out theoretically at 915 MHz. Thus, the proposed differential extraoral systems can be employed to acquire and transmit the user intentions in eTDS inspired healthcare applications., (© 2024. The Author(s).)

Chikungunya (CHIKV) and Mayaro (MAYV) viruses are arthritogenic alphaviruses that promote an incapacitating and long-lasting inflammatory muscle-articular disease. Despite studies pointing out the importance of skeletal muscle (SkM) in viral pathogenesis, the long-term consequences on its physiology and the mechanism of persistence of symptoms are still poorly understood. Combining molecular, morphological, nuclear magnetic resonance imaging, and histological analysis, we conduct a temporal investigation of CHIKV and MAYV replication in a wild-type mice model, focusing on the impact on SkM composition, structure, and repair in the acute and late phases of infection. We found that viral replication and induced inflammation promote a rapid loss of muscle mass and reduction in fiber cross-sectional area by upregulation of muscle-specific E3 ubiquitin ligases MuRF1 and Atrogin-1 expression, both key regulators of SkM fibers atrophy. Despite a reduction in inflammation and clearance of infectious viral particles, SkM atrophy persists until 30 days post-infection. The genomic CHIKV and MAYV RNAs were still detected in SkM in the late phase, along with the upregulation of chemokines and anti-inflammatory cytokine expression. In agreement with the involvement of inflammatory mediators on induced atrophy, the neutralization of TNF and a reduction in oxidative stress using monomethyl fumarate, an agonist of Nrf2, decreases atrogen expression and atrophic fibers while increasing weight gain in treated mice. These data indicate that arthritogenic alphavirus infection could chronically impact body SkM composition and also harm repair machinery, contributing to a better understanding of mechanisms of arthritogenic alphavirus pathogenesis and with a description of potentially new targets of therapeutic intervention.

This paper presents the design and analysis of a metamaterial-based compact dual-band antenna for WBAN applications. The antenna is designed and fabricated on a 0.254 mm thick semi-flexible substrate, RT/Duroid® 5880, with a relative permittivity of 2.2 and a loss tangent of 0.0009. The total dimensions of the antenna are 0.26λo×0.19λo×0.002λo, where λo corresponds to the free space wavelength at 2.45 GHz. To enhance overall performance and isolate the antenna from adverse effects of the human body, it is backed by a 2×2 artificial magnetic conductor (AMC) plane. The total volume of the AMC integrated design is 0.55λo×0.55λo×0.002λo. The paper investigates the antenna's performance both with and without AMC integration, considering on- and off-body states, as well as various bending conditions in both E and H-planes. Results indicate that the AMC-integrated antenna gives improved measured gains of 6.61 dBi and 8.02 dBi, with bandwidths of 10.12% and 7.43% at 2.45 GHz and 5.80 GHz, respectively. Furthermore, the AMC integrated antenna reduces the specific absorption rate (SAR) to (>96%) and (>93%) at 2.45 GHz and 5.80 GHz, meeting FCC requirements for low SAR at both frequencies when placed in proximity to the human body. CST Microwave Studio (MWS) and Ansys High-Frequency Structure Simulation (HFSS), both full-wave simulation tools, are utilized to evaluate the antenna's performance and to characterize the AMC unit cell. The simulated and tested results are in mutual agreement. Due to its low profile, high gain, adequate bandwidth, low SAR values, and compact size, the AMC integrated antenna is considered suitable for WBAN applications., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 Ali et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

The sealing capacity of magnetofluidic (MF) rotating seals is limited by the highest magnetization of the sealing ferrofluids (FFs) of about 1000 G (80 kA m -1 ). A sharp, almost an order of magnitude, increase in the supported pressure drop is possible by using a magnetorheological (MR) suspension as sealing fluid, owing to the much higher saturation magnetization of MR fluids compared to FFs. However, rotating seals with MR fluids have several shortcomings, such as a significant increase of the friction torque due to the growth of shear stress in the strong magnetic field specific to MF seals and leakage of the non-magnetic carrier liquid. At least partly, these issues can be avoided by using ferrofluid based extremely bidisperse MR suspensions of micrometer-sized iron (Fe) particles dispersed in a ferrofluid, as sealing fluid. The composite Fe 3 O 4 -Fe magnetic fluid used in this study consisted of hundreds of nanometers up to few microns size structures of interconnected (welded) Fe nanoparticles (FeMNPs) dispersed in a high colloidal stability ferrofluid with 500 G (40 kA m -1 ) saturation magnetization. The volume fraction of iron NPs varies from 0.5 to 15% in the ferrofluid carrier which significantly increases the magnetization and simultaneously produces important changes of flow properties in magnetic field of the resulting composite fluid, from Newtonian to strongly non-Newtonian behavior. The evaluation of the magnetic and magnetorheological behavior includes the dependence of magnetization, effective viscosity, magnetoviscous effect and dynamic yield stress on the volume fraction of Fe nanoparticles dispersed in the ferrofluid carrier. The seal gap filled with interconnected Fe nanospheres consists in randomly distributed microregions with a high intensity and high gradient magnetic field that captures the ferrofluid and provides a new sealing mechanism. Already a small amount of interconnected Fe nanospheres additive (2.5-5.0% volume fraction) produces four times increase of the rotating seal burst pressure, a much higher increase than what can be obtained from using a conventional magnetic fluid with the same magnetization. The nano-composite sealing magnetic fluid proved to be a cost-effective solution to significantly increase the performance of multi-stage rotating MF seals.

This paper proposes a novel multi-band textile monopole antenna for patient tracking applications. The designed antenna has compact footprints (0.13 λ 0 2 ) and works in the narrow band-internet of things (NB-IoT) 1.8 GHz, radio frequency identification (RFID), and industrial, scientific, and medical (ISM) 2.45 GHz and 5.8 GHz bands. The impedance bandwidths and gain of the antenna at 1.8 GHz, 2.45 GHz, and 5.8 GHz are 310 MHz, 960 MHz, and 1140 MHz; 3.7 dBi, 5.3 dBi, and 9.6 dBi, respectively. Also, the antenna's behavior is checked on different body parts of the human body in various bending scenarios. As per the evaluated link budget, the designed antenna can easily communicate up to 100 m of distance. The specific absorption rate values of the designed antenna are also within acceptable limits as per the (FCC/ICNIRP) standards at the reported frequency bands. Unlike traditional rigid antennas, the proposed textile antenna is non-intrusive, enhancing user safety and comfort. The denim material makes it comfortable for extended wear, reducing the risk of skin irritation. It can also withstand regular wear and tear, including stretching and bending. The presented denim-based antenna can be seamlessly integrated into clothing and accessories, making it less obtrusive and more aesthetically pleasing.

Today wireless systems include the fifth and sixth generations (5G and 6G) technologies and are growing day by day that result in exponentially increasing data traffic. For providing a reliable and high performance radio frequency (RF) designs especially for 6G networks, amplifiers and antenna as active and passive components play important roles. In the 5G/6G communication systems, the propagation loss is considerably large and its compensation requires high output power generated from the amplifiers for guaranteeing the satisfied quality of transmitted signal. From another point of view, the installed antennas must be able to optimally manage the radiated signals and handle/compensate nonlinear performances of the RF circuitry. Hence, advanced modeling and multi-objective optimization algorithms are required for designing and optimizing high performance amplifiers and antennas in terms of output power, gain, efficiency, linearity, and bandwidth. Concurrently optimizing active and passive components is not straightforward and typically it requires additional efforts by the RF designers. To tackle this drawback, a two-step methodology is proposed: (1) configuring the initial structure of active and passive devices, and (2) sizing the configured devices. In this work, various methods are introduced for structuring the topology of circuits and then artificial intelligence, including machine learning and neural networks, is preferred among other surrogate modelling for sizing the designs. These neural networks are satisfied due to the accurate modeling responses and are able to provide an automated optimization process leads to employ multi-objective optimization methods. In this work, an automated optimization process for comprehensive design of high-performance amplifiers with antennas through bottom-up optimization (BUO) method and long short-term memory (LSTM)-based deep neural networks (DNNs) is proposed. At the output layer of DNNs, the multi-objective multi-verse optimizer (MOMVO) method is employed for optimizing various specifications of active device (i.e., amplifier), and passive device (i.e., antenna), concurrently. In the presented method, all the electromagnetic (EM) design rules are implemented which results in reducing simulation time in the harmonic balance simulation environment that also provides ready to fabricate layouts. The novelty consists of the all-inclusive style that (1) reduces the manual breaks, aka time-to-market, and (2) delivers ready-to-fabricate layouts of the device that exhibits global optimum performances, automatically. The validation of the proposed method is verified by designing and optimizing high power amplifier (HPA) with antenna in the frequency band from 9.0 GHz to 9.6 GHz, suitable for upper-mid band 6G communications., (© 2024. The Author(s).)

The design and experimental verification of a deeply implanted conformal printed antenna is presented. The hip implant acts as the ground plane for a coaxial-cable-fed trapezoidal radiator designed to transmit biological signals collected within the body by proper biosensors. The arrangement, consisting of a metallic (or equivalent) hip implant, bio-compatible gypsum-based dielectric, and conformal radiator, was tested when the hosting 3D-printed plastic bone was immersed in tissue-like liquid contained in a plastic bucket. The dimensions of the set-up are similar to a human leg. Matching and radiation characteristics are presented in the industrial, scientific, and medical (ISM) frequency band (2.4-2.5 GHz), showing the feasibility of the proposed arrangement.

Forest restoration mitigates climate change by removing CO 2 and storing C in terrestrial ecosystems. However, incomplete information on C storage in restored tropical forests often fails to capture the ecosystem's holistic C dynamics. This study provides an integrated assessment of C storage in above to belowground subsystems, its consequences for greenhouse gas (GHG) fluxes, and the quantity, quality, and origin of soil organic matter (SOM) in restored Atlantic forests in Brazil. Relations between SOM properties and soil health indicators were also explored. We examined two restorations using tree planting ('active restoration'): an 8-year-old forest with green manure and native trees planted in two rounds, and a 15-year-old forest with native-planted trees in one round without green manure. Restorations were compared to reformed pasture and primary forest sites. We measured C storage in soil layers (0-10, 10-20, and 20-30 cm), litter, and plants. GHG emissions were assessed using CH 4 and CO 2 fluxes. SOM quantity was evaluated using C and N, quality using humification index (H LIFS ), and origin using δ 13 C and δ 15 N. Nine soil health indicators were interrelated with SOM attributes. The primary forest presented the highest C stocks (107.7 Mg C ha -1 ), followed by 15- and 8-year-old restorations and pasture with 69.8, 55.5, and 41.8 Mg C ha -1 , respectively. Soil C stocks from restorations and pasture were 20% lower than primary forest. However, 8- and 15-year-old restorations stored 12.3 and 28.3 Mg ha -1 more aboveground C than pasture. The younger forest had δ 13 C and δ 15 N values of 2.1 and 1.7‰, respectively, lower than the 15-year-old forest, indicating more C derived from C 3 plants and biological N fixation. Both restorations and pasture had at least 34% higher H LIFS in deeper soil layers (10-30 cm) than primary forest, indicating a lack of labile SOM. Native and 15-year-old forests exhibited higher soil methane influx (141.1 and 61.9 μg m -2 h -1 ). Forests outperformed pasture in most soil health indicators, with 69% of their variance explained by SOM properties. However, SOM quantity and quality regeneration in both restorations approached the pristine forest state only in the top 10 cm layer, while deeper soil retained agricultural degradation legacies. In conclusion, active restoration of the Atlantic Forest is a superior approach compared to pasture reform for GHG mitigation. Nonetheless, the development of restoration techniques to facilitate labile C input into deeper soil layers (>10 cm) is needed to further improve soil multifunctionality and long-term C storage., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Beam-switching is one of the paramount focuses of 28 GHz millimeter-wave 5G devices. In this paper, a one-dimensional (1D) pattern reconfigurable leaky-wave antenna (LWA) was investigated and developed for wireless terminals. In order to provide a cost-effective solution, a uniform half-width LWA was used. The 1D beam-switching LWA was designed using three feed points at three different positions; by selecting the feeds, the direction of the beam can be switched. The antenna can switch the beam in three different directions along the antenna axis, such as backward, broadside, and forward. The 1D beam-switching antenna was fabricated, and because of the wide beamwidth, the measured radiation patterns can fill 128∘ of space (3 dB coverage), from θ = -64∘ to +64∘ at ϕ = 0∘. Following this, two of these antennas were placed at right angles to each other to achieve two-directional (2D) beam switching. The 2D beam-switching antenna pair was also prototyped and tested after integrating them into the ground plane of a wireless device. The antenna is able to point the beam in five different directions; moreover, its beam covers 167∘ (θ = -89∘ to +78∘) at ϕ = 0∘, and 154∘ (θ = -72∘ to +82∘) at ϕ = 90∘.

Assistive devices are becoming increasingly popular for physically disabled persons suffering tetraplegia and spinal cord injuries. Intraoral tongue drive system (iTDS) is one of the most feasible and non-invasive assistive technology (AT), which utilises the transferring and inferring of user intentions through different tongue gestures. Wireless transferring is of prime importance and requires a suitable design of the intra-oral antenna. In this paper, a compact circularly polarized differential intra-oral antenna is designed, and its performance is analysed within heterogeneous multilayer mouth and head models. It works at 2.4 GHz in the Industrial, Scientific, and Medical (ISM) band. The footprint of the differential antenna prototype is 0.271 λ g [Formula: see text] 0.271 λ g [Formula: see text] 0.015 λ g . It is achieved using two pairs of spiral segments loaded in diagonal form near the edges of the central rotated square slot and a high dielectric constant substrate. Its spiral-slotted geometry further provides the desired swirling and miniaturization at the desired frequency band for both mouth scenarios. Additionally, corner triangular slits on the radiating patch assist in tuning the axial ratio (< 3 dB) in the desired ISM band. To validate the performance of the proposed in-mouth antenna, the measurement was carried out using the minced pork and the saline solution for closed and opened mouth cases, respectively. The measured - 10 dB impedance bandwidth and peak gain values in the minced pork are from 2.28 to 2.53 GHz (10.39%) and - 18.17 dBi, respectively, and in the saline solution, are from 2.3 to 2.54 GHz (9.92%) and - 15.47 dBi, respectively. Further, the specific absorption rate (SAR) is estimated, and the data communication link is computed with and without a balun loss. This confirms that the proposed differential intraoral antenna can establish direct interfacing at the RF front end of the intraoral tongue drive system., (© 2023. The Author(s).)

Cardiovascular diseases (CVDs) are one of the leading causes of death globally. The Internet of things (IoT) enabled with industrial, scientific, and medical (ISM) bands (2.45 and 5.8 GHz) facilitates pacemakers to remotely share heart health data to medical professionals. For the first time, communication between a compact dual-band two-port multiple-input-multiple-output (MIMO) antenna (integrated inside the leadless pacemaker) and an outside-body dual-band two-port MIMO antenna in the ISM 2.45 and 5.8 GHz frequency bands is demonstrated in this work. The proposed communication system offers an attractive solution for cardiac pacemakers as it can operate on a 5G IoT platform while also being compatible with existing 4G standards. The experimental verification of the proposed MIMO antenna low-loss communication capability is also presented by comparing it to the existing single-input-single-output communication between the leadless pacemaker and outside body monitoring device., (© 2023. The Author(s).)

Tunable/switchable devices are more and more required in modern communication systems. However, the realization of the tuning requires the presence of active devices, which in turn must be biased. The current paper comes up with a solution for designing and experimentally validating such a switchable Frequency Selective Surface. Two different metallic structures are simulated and measured, having incorporated the same topology control network (CN). In this scenario, the main innovation of this paper is the presence of the feeding part, namely the control network. In this work, the main FSS structure is flanked by three parallel CN microstrip lines and several via holes that allow biasing the active elements, namely PIN diodes. The switchability of the proposed structure is achieved through PIN diodes, whose bias determines the values of the elements in the equivalent circuit. At different biases, the response of the FSS changes accordingly. From all possible values of the bias, the extreme cases when the diodes act as (almost) short- and open-circuits are considered in the submitted manuscript for the sake of brevity. These cases are modeled by the main and cut-slot structures, respectively. The proposed structures have been evaluated using electromagnetic simulation and implemented on an FR4 substrate having a thickness of 1.58 mm. With the periodicity of the square-shaped unit cell of 18 mm edge length, different filtering bands are obtained below 12 GHz. Another novelty that has received very little consideration in the existing literature is the use of a finite array of unit cells instead of an infinite one. And finally, tests in an anechoic chamber have proved that there is a good agreement between practical and simulation results and also demonstrated the proper performance of the devices for wide angular incidence for both TE and TM polarizations.

Despite significant progress in robot hardware, the number of mobile robots deployed in public spaces remains low. One of the challenges hindering a wider deployment is that even if a robot can build a map of the environment, for instance through the use of LiDAR sensors, it also needs to calculate, in real time, a smooth trajectory that avoids both static and mobile obstacles. Considering this scenario, in this paper we investigate whether genetic algorithms can play a role in real-time obstacle avoidance. Historically, the typical use of genetic algorithms was in offline optimization. To investigate whether an online, real-time deployment is possible, we create a family of algorithms called GAVO that combines genetic algorithms with the velocity obstacle model. Through a series of experiments, we show that a carefully chosen chromosome representation and parametrization can achieve real-time performance on the obstacle avoidance problem.

We propose and develop a novel rigorous technique that enables one to obtain the explicit numerical values of parameters at which several lowest-order harmonics of the scattered field are suppressed. This provides partial cloaking of the object, a perfectly conducting cylinder of circular cross section covered by two layers of dielectric separated by an infinitely thin impedance layer, a two-layer impedance Goubau line (GL). The developed approach is a rigorous method that enables one to obtain in the closed form (and without numerical calculations) the values of parameters providing the cloaking effect, achieved particularly in terms of the suppression of several scattered field harmonics and variation of the sheet impedance. This issue constitutes the novelty of the accomplished study. The elaborated technique could be applied to validate the results obtained by commercial solvers with virtually no limitations on the parameter ranges, i.e., use it as a benchmark. The determination of the cloaking parameters is straightforward and does not require computations. We perform comprehensive visualization and analysis of the achieved partial cloaking. The developed parameter-continuation technique enables one to increase the number of the suppressed scattered-field harmonics by appropriate choice of the impedance. The method can be extended to any dielectric-layered impedance structures possessing circular or planar symmetry.

Continuous monitoring and treatment of various diseases with biomedical technologies and wearable electronics has become significantly important. The healthcare area is an important, evolving field that, among other things, requires electronic and micro-electromechanical technologies. Designed circuits and smart devices can lead to reduced hospitalization time and hospitals equipped with high-quality equipment. Some of these devices can also be implanted inside the body. Recently, various implanted electronic devices for monitoring and diagnosing diseases have been presented. These instruments require communication links through wireless technologies. In the transmitters of these devices, power amplifiers are the most important components and their performance plays important roles. This paper is devoted to collecting and providing a comprehensive review on the various designed implanted amplifiers for advanced biomedical applications. The reported amplifiers vary with respect to the class/type of amplifier, implemented CMOS technology, frequency band, output power, and the overall efficiency of the designs. The purpose of the authors is to provide a general view of the available solutions, and any researcher can obtain suitable circuit designs that can be selected for their problem by reading this survey.

The present paper introduces an optimization-oriented method here practiced for designing high performance single antennas in a fully automated environment. The proposed method comprises two sequential major steps. The first one devotes configuring the shape of antenna and determining the feeding point by employing the bottom-up optimization (BUO) method. In this algorithm, the number of microstrip transmission lines (TLs) used to model the radiator is increased consecutively and the shape of the antenna is revised up to finding the initial satisfying results. Secondly, for determining the best design parameters of the configured antenna shape in the first step (i.e., width and length of TLs), deep neural network (DNN) that is based on Thompson sampling efficient multi-objective optimization (TSEMO) is applied. The recommended optimization method is successfully attracted as a problem solver for designers to tackle the subject for antenna design such as the complexity and large dimensions of structures. Hence, the main advantage of the implemented optimization method in this article is to noticeably decrease the required designer's involvement automatically generating valid layouts. For validating the suggested method, two wideband antennas are designed, prototyped and subjected to experiment. The first optimized antenna covers the frequency band 8.8-10.1 GHz (43 % bandwidth) characterized by a maximum gain of 7.13 dB while the second one covers the frequency band 11.3-13.16 GHz (47.5 %) which exhibits a maximum gain of 7.8 dB., (© 2022. The Author(s).)

This paper deals with the design and fabrication of an unpretentious (single-layer, without any lump element) broadband (97%, 11.3-32.3 GHz) radar cross-section reduction (RCSR) modulated surface (MS). The proposed structure uses sinusoidal modulation gap sizes between square patches within square unit cells to form a phase gradient that plays an effective role in improving the RCSR bandwidth. An MS with dimensions of 250 × 250 mm 2 , consisting of 40 × 40 unit cells with a period of 6 mm printed on a RO4003C (lossy) substrate of 0.06λ LF ( λ LF being the wavelength at the lower frequency) thickness, has been prototyped. The MS has square patch (SP) unit cells with seven different gap sizes. A genetic algorithm (GA)-based fine-tuning has been implemented to further increase the performances of the structure. Measurements on it have been conducted considering both mono- and bi-static arrangements and for oblique incidences for both TM and TE polarization tests. A good agreement between simulation and measurement results proves the validity of the design criteria.

Drug therapy for vascular disease has been promoted to inhibit angiogenesis in atherosclerotic plaques and prevent restenosis following surgical intervention. This paper investigates the arterial depositions and distribution of PEG-functionalized magnetic nanocomposite clusters (PEG&#95;MNCs) following local delivery in a stented artery model in a uniform magnetic field produced by a regionally positioned external permanent magnet; also, the PEG&#95;MNCs aggregation or chain formation in and around the implanted stent. The central concept is to employ one external permanent magnet system, which produces enough magnetic field to magnetize and guide the magnetic nanoclusters in the stented artery region. At room temperature (25 °C), optical microscopy of the suspension model's aggregation process was carried out in the external magnetic field. According to the optical microscopy pictures, the PEG&#95;MNC particles form long linear aggregates due to dipolar magnetic interactions when there is an external magnetic field. During magnetic particle targeting, 20 mL of the model suspensions are injected (at a constant flow rate of 39.6 mL/min for the period of 30 s) by the syringe pump in the mean flow (flow velocity is Um = 0.25 m/s, corresponding to the Reynolds number of Re = 232) into the stented artery model. The PEG&#95;MNC clusters are attracted by the magnetic forces (generated by the permanent external magnet) and captured around the stent struts and the bottom artery wall before and inside the implanted stent. The colloidal interaction among the MNC clusters was investigated by calculating the electrostatic repulsion, van der Waals and magnetic dipole-dipole energies. The current work offers essential details about PEG&#95;MNCs aggregation and chain structure development in the presence of an external magnetic field and the process underlying this structure formation.

Soil organic matter (SOM) plays a key role in the global carbon and nitrogen cycles. Soil biogeochemistry is regularly studied by extracting the base-soluble fractions of SOM: acid-insoluble humic acid (HA) and acid-soluble fulvic acid (FA). Electrospray ionization-Fourier transform-ion cyclotron resonance-mass spectrometry (ESI-FT-ICR-MS) is commonly utilized for molecularly characterizing these fractions. Different sample preparation techniques exist for the analysis of HA and FA though questions remain regarding data comparability following different preparations. Comparisons of different sample preparation techniques here revealed that the negative-mode ESI-FT-ICR-MS analytical window can be skewed to detect different groups of molecules, with primary differences in oxygenation, aromaticity, and molecular weight. It was also observed that HA and FA from soils versus an aquatic matrix behaved very differently. Thus, we conclude that sample preparation techniques determined to be "most optimal" in our study are in no way universal. We recommend that future studies of HA and FA involve similar comparative studies for determining the most suitable sample preparation technique for their particular type of HA or FA matrices. This will enhance data comparability among different studies and environmental systems and ultimately allow us to better understand the complex composition of environmental matrices.

In this study, we focus on automated optimization design methodologies to concurrently trade off between power gain, output power, efficiency, and linearity specifications in radio frequency (RF) high-power amplifiers (HPAs) through deep neural networks (DNNs). The RF HPAs are highly nonlinear circuits where characterizing an accurate and desired amplitude and phase responses to improve the overall performance is not a straightforward process. For this case, we propose a coarse and fine modeling approach based on firstly modeling the involved transistor and then selecting the best configuration of HAP along with optimizing the involved input and output termination networks through DNNs. In the fine phase , we firstly construct the equivalent modeling of the GaN HEMT transistor by using X-parameters. Then in the coarse phase , we utilize hidden layers of the modeled transistor and replace the HPA's DNN to model the behavior of the selected HPA by using S-parameters. If the suitable accuracy of HPA modeling is not achieved, the hyperparameters of the fine model are improved and re-evaluated in the HPA model. We call the optimization process coarse and fine modeling since the evaluation process is performed from S-parameters to X-parameters. This stage of optimization can ensure modeling the nonlinear HPA design that includes a high number of parameters in an effective way. Furthermore, for accelerating the optimization process, we use the classification DNN for selecting the best topology of HPA for modeling the most suitable configuration at the coarse phase. The proposed modeling strategy results in relatively highly accurate HPA designs that generate post-layouts automatically, where multi-tone harmonic balance specifications are optimized once together without any human interruptions. To validate the modeling approach and optimization process, a 10 W HPA is simulated and measured in the operational frequency band of 1.8 GHz to 2.2 GHz, i.e., the L-band. The measurement results demonstrate a drain efficiency higher than 54% and linear gain performance more than 12.5 dB, with better than 50 dBc adjacent channel power ratio (ACPR) after DPD.

Background: Urinary tract infections (UTIs) are among the most common bacterial infections worldwide and have become more difficult to treat over the years. Inappropriate antibiotic use has led to increased antibiotic resistance., Materials and Methods: We examined 1921 urine culture samples from a single hospital and analyzed them for bacterial spectrum and antibiotic susceptibility. We further analyzed changes in the rates of detected bacteria and of the sensitivity of these uropathogens to antibiotics over the years., Results: In our hospital-based analysis, cystitis was the most frequently diagnosed UTI in women (76%) and men (79%). Escherichia coli (48%) was the most commonly identified uropathogen. Samples demonstrated an increase in the proportion of E. coli (p   <  0.001) and a decrease in Enterococcus faecalis (p   <  0.001) over the study time period. Antimicrobial susceptibility analysis showed an increase over time in the number of isolates with resistance to ampicillin/sulbactam (p   <  0.001) and to third-generation cephalosporins cefotaxime (p   =  0.043) and ceftazidime (p   <  0.001)., Conclusions: Ampicillin/sulbactam and third-generation cephalosporins are antibiotics frequently used in the treatment of UTIs. When selecting an optimal antimicrobial treatment regimen for patients with UTIs, it is imperative to understand regional and timedependent differences in the prevalence of various uropathogens and antimicrobial resistance patterns. Therefore, continuous surveillance of local pathogen and antimicrobial susceptibility patterns for frequently used antibiotics should be prioritized., Competing Interests: The authors have no conflicts of interest to declare., (Copyright © 2022 The Authors. Published by Wolters Kluwer Health, Inc.)

This paper presents a design of multiple input multiple output (MIMO) antenna array for 5G millimeter-wave (mm-wave) communication systems. The proposed MIMO configuration consists of a two antenna arrays combination. Each antenna array consists of four elements which are arranged in an even manner, while two arrays are then assembled with a 90-degree shift with respect to each other. The substrate used is a 0.254 mm thick Rogers RT5880 with a dielectric constant of 2.2 and loss tangent of 0.0009, correspondingly. The proposed MIMO antenna array covers the 37 GHz frequency band, dedicated for 5G millimeter-wave communication applications. The proposed antenna element yields a gain of 6.84 dB, which is enhanced up to 12.8 dB by adopting a four elements array configuration. The proposed MIMO antenna array performance metrics, such as envelope correlation coefficient (ECC) and diversity gain (DG), are observed, which are found to be under the standard threshold. More than 85% of the radiation efficiency of the proposed MIMO antenna array is observed to be within the desired operating frequency band. All the proposed designs are simulated in computer simulation technology (CST) software. Furthermore, the measurements are carried out for the proposed MIMO antenna array, where a good agreement with simulated results is observed. Thus, the proposed design can be a potential candidate for 5G millimeter-wave communication systems.

Understanding the chemical make-up of soils and their structure is critical for constraining the role of soil organic matter (SOM) into the global biogeochemical cycles, as well as to understand the capability of SOM to sequester carbon and mitigate greenhouse gas emissions. Here, we use two-dimensional 1 H- 13 C heteronuclear single quantum coherence nuclear magnetic resonance (2D 1 H- 13 C HSQC NMR) spectroscopy to structurally characterize the most refractory component of SOM, the humic acid (HA). The observations from 2D 1 H- 13 C HSQC NMR were coupled with lignin phenol and fatty acid measurements using tetramethylammonium hydroxide (TMAH) thermochemolysis - two-dimensional gas chromatography - mass spectrometry (TMAH-GC × GC-MS). We studied humic acids extracted from an integrated Crop - Livestock - Forest System (CLFS) agricultural area and an undisturbed Atlantic Native Forest (NF) area. We evaluated soils from two different depths: the topsoil (0-20 cm) and subsoil (60-100 cm) layers, and reveal the presence of oxidized ligninaceous phenols as we had previously hypothesized. Collectively, our results indicate that there are significant oxidative processes with increasing soil depth which are more pronounced in the CLFS relative to the NF area. Degradation of stearic acid with increasing depth in the CLFS soils indicated that the CLFS is more microbiologically active than NF. Therefore, CLFS is less biochemically stable than we originally perceived. The enhanced bio-reactivity of CLFS is likely driving the enhanced carbon sequestration in the CLFS soils. This is perhaps due to the diversity of biomass remnants available at the CLFS soil rhizosphere which allows for more different types of biomass to be sequestered as oxidized ligninaceous phenols. Our results employing structural characterization with 2D 1 H- 13 C HSQC NMR and TMAH-GC × GC-MS provide a new layer of knowledge about the practice of integrated agricultural systems and allow us to understand the structure and fate of sequestered carbon in soils from different soil environments., Competing Interests: Declaration of competing interest There are no conflicts of interest or relationship, financial or otherwise that might be perceived as influencing the objectivity for this research., (Copyright © 2021 Elsevier B.V. All rights reserved.)

A frequency selective surface for spatial filtering in the standardized Ultra-Wide Band (UWB) frequency range is proposed. A very large stop-band of 1.75-15.44 GHz has been obtained, with good polarization insensitivity and an angular stability of more than 60∘ and more than 50∘ in TE and TM incidence, respectively. Circuit models have been devised. The structure has been assessed by electromagnetic simulation and implemented on an FR4 substrate of 1.6 mm thickness, with an edge of the square-shaped unit cell of 15 mm. Tests in an anechoic chamber demonstrated good matching between simulation and experimental results and proper operation of the device.

The leadless cardiac pacemaker is a pioneering device for heart patients. Its rising success requires the design of compact implantable antennas. In this paper, we describe a circularly polarized Hilbert curve inspired loop antenna. The proposed antenna works in the WMTS (Wireless Medical Telemetry Services) 1.4 GHz and ISM (Industrial, Scientific, and Medical) 2.45 GHz bands. High dielectric constant material Rogers RT/Duroid 6010 LM ([Formula: see text]=10) and fractal geometry helps to design the antenna with a small footprint of 9.1 mm 3 (6 mm × 6 mm × 0.254 mm). The designed antenna has a conformal shape that fits inside a leadless pacemaker's capsule is surrounded by IC models and battery, which are tightly packed in the device enclosure. Subsequently, the integrated prototype is simulated deep inside at the center of the multi-layer canonical heart model. To verify experimentally, we have put dummy electronics (IC and battery) inside the 3D printed pacemaker's capsule and surfaced the fabricated conformal antenna around the inner curved body of the TCP (Transcatheter Pacing) capsule. Furthermore, we have tested the TCP capsule by inserting it in a ballistic gel phantom and minced pork. The measured impedance bandwidths at 1.4 GHz and 2.45 GHz are 250 MHz and 430 MHz, whereas measured gains are - 33.2 dBi, and - 28.5 dBi, respectively., (© 2022. The Author(s).)

Today, public health is one of the most important challenges in society. Cancer is the leading cause of death, so early diagnosis and localized treatments that minimize side effects are a priority. Magnetic nanoparticles have shown great potential as magnetic resonance imaging contrast agents, detection tags for in vitro biosensing, and mediators of heating in magnetic hyperthermia. One of the critical characteristics of nanoparticles to adjust to the biomedical needs of each application is their polymeric coating. Fatty acid coatings are known to contribute to colloidal stability and good surface crystalline quality. While monolayer coatings make the particles hydrophobic, a fatty acid double-layer renders them hydrophilic, and therefore suitable for use in body fluids. In addition, they provide the particles with functional chemical groups that allow their bioconjugation. This work analyzes three types of self-assembled bilayer fatty acid coatings of superparamagnetic iron oxide nanoparticles: oleic, lauric, and myristic acids. We characterize the particles magnetically and structurally and study their potential for resonance imaging, magnetic hyperthermia, and labeling for biosensing in lateral flow immunoassays. We found that the myristic acid sample reported a large r2 relaxivity, superior to existing iron-based commercial agents. For magnetic hyperthermia, a significant specific absorption rate value was obtained for the oleic sample. Finally, the lauric acid sample showed promising results for nanolabeling.

Humic substances (HS) possess redox active groups covering a wide range of potentials and are used by facultative anaerobic microorganisms as electron acceptors. To serve as suitable electron shuttles for anaerobic respiration, HS should be able to re-oxidize relatively quickly to prevent polarization of the surrounding medium. Mediated electrochemical oxidation and decolorization assays, based on the reduction of the radical ion of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS •- ) allow to determine the electron donating capacity (EDC) of HS, but uncertainties remain about the reaction time that should be allowed to obtain environmentally meaningful EDC values. In this work, we performed a kinetic analysis of the time trend of the reduction of ABTS •- by HS by Vis and Electron Paramagnetic Resonance (EPR) spectroscopies and by cyclic voltammetry. We found evidences of two concomitant separate mechanisms of electron exchange: a fast and a slow transfer processes which may have different environmental roles. These results can set a base to identify the appropriate conditions for the spectrophotometric determination of the fast and slow components of the EDC of HS., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Two soils contaminated with potentially toxic metals (PTMs) contrasting in pH and mineralogy were remediated with CaEDTA, and changes in soil organic matter (SOM) composition were investigated. Previous studies showed no significant loss of SOM from CaEDTA-treated soils, but the results of our study reflected significant decreases (from 46 to 49%) in the free fraction of humic acids (HAs). Remediation affected the composition of the free HA fraction via disturbance of intermolecular bonds - an increase in phenolic and aromatic groups with a simultaneous decrease in carbohydrates - which was confirmed by FTIR spectroscopy in both soils. Because non-radical molecules such as carbohydrates were selectively removed, the concentration of free radicals in the free HA fraction increased in acidic soil. The bound fraction of HAs and fulvic acids (FAs) in SOM, which are important due to their stability and the permanent effects they have on the soil's physical properties, remained unchanged in both remediated soils. The effect of soil recultivation was observed only in the excitation emission matrix (EEM) fluorescence spectra of HAs. In terms of SOM, CaEDTA soil washing can be considered moderately conservative; however, the restoration of free humic fractions is likely to be a long-term process., (© 2021. The Author(s).)

Zika virus (ZIKV) infection became a worldwide concern due to its correlation with the development of microcephaly and other neurological disorders. ZIKV neurotropism is well characterized, but the role of peripheral viral amplification to brain infection remains unknown. Here, we found that ZIKV replicates in human primary skeletal muscle myoblasts, impairing its differentiation into myotubes but not interfering with the integrity of the already-formed muscle fibers. Using mouse models, we showed ZIKV tropism to muscle tissue either during embryogenesis after maternal transmission or when infection occurred after birth. Interestingly, ZIKV replication in the mouse skeletal muscle started immediately after ZIKV inoculation, preceding viral RNA detection in the brain and causing no disruption to the integrity of the blood brain barrier, and remained active for more than 2 weeks, whereas replication in the spleen and liver were not sustained over time. In addition, ZIKV infection of the skeletal muscle induces necrotic lesions, inflammation, and fiber atrophy. We also found a reduction in the expression of regulatory myogenic factors that are essential for muscle repair after injury. Taken together, our results indicate that the skeletal muscle is an early site of viral amplification and lesion that may result in late consequences in muscle development after ZIKV infection. IMPORTANCE Zika Virus (ZIKV) neurotropism and its deleterious effects on central nervous system have been well characterized. However, investigations of the initial replication sites for the establishment of infection and viral spread to neural tissues remain underexplored. A complete description of the range of ZIKV-induced lesions and others factors that can influence the severity of the disease is necessary to prevent ZIKV's deleterious effects. ZIKV has been shown to access the central nervous system without significantly affecting blood-brain barrier permeability. Here, we demonstrated that skeletal muscle is an earlier site of ZIKV replication, contributing to the increase of peripheral ZIKV load. ZIKV replication in muscle promotes necrotic lesions and inflammation and also impairs myogenesis. Overall, our findings showed that skeletal muscle is involved in pathogenesis and opens new fields in the investigation of the long-term consequences of early infection.

In modern smarthomes, temperature regulation is achieved through a mix of traditional and emergent technologies including air conditioning, heating, intelligent utilization of the effects of sun, wind, and shade as well as using stored heat and cold. To achieve the desired comfort for the inhabitants while minimizing environmental impact and cost, the home controller must predict how its actions will impact the temperature and other environmental factors in various parts of the home. The question we are investigating in this paper is whether the temperature values in different rooms in a home are predictable based on readings from sensors in the home. We are also interested in whether increased accuracy can be achieved by adding sensors to capture the state of doors and windows of the given room and/or the whole home, and what type of machine learning algorithms can take advantage of the additional information. As experimentation on real-world homes is highly expensive, we use ScaledHome, a 1:12 scale, IoT-enabled model of a smart home for data acquisition. Our experiments show that while additional data can improve the accuracy of the prediction, the type of machine learning models needs to be carefully adapted to the number of data features available.

Purpose: Breast cancer is the most common malignancy in Brazilian women, with 66,280 new cases in 2020 (with 20% overexpressing human epidermal growth factor receptor 2 [HER2]). The trastuzumab biosimilar was the first oncology biosimilar approved in Brazil for HER2-positive breast cancer treatment. This study aimed to assess the current level of knowledge of biosimilars, comfort of use, extrapolation indications, and switching of practices among oncologists in Brazil., Methods: A 24-question survey was developed using an online platform that sought information regarding responders' characteristics and use of biosimilars. The survey analyzed the basic knowledge of biosimilars, trastuzumab biosimilars, level of comfort with extrapolation, switching treatment regimens, and opinions concerning the cost of HER2-positive breast cancer therapy. Data were collected between July and September 2019 and included 144 oncologists from five Brazilian regions., Results: In total, 95% of respondents could identify the most appropriate definition of biosimilars and 96% felt comfortable prescribing trastuzumab biosimilars. Although 63% of respondents would use the biosimilar in all settings wherein the reference biologic was approved, 35% would use the biosimilar for cases involving metastatic disease. Although 82% of oncologists were in favor of switching from a reference biologic to a biosimilar, 18% would avoid switching regimens. The lack of studies detailing switching to other regimens and the correct timing to switch was the major concern. The cost of HER2 therapy was a significant concern for most oncologists., Conclusion: Oncologists demonstrated a high level of knowledge of biosimilars and encouraging levels of prescriber use; however, extrapolation and switching treatment regimens are barriers to the effective use of biosimilars in cancer treatment. Efforts should be concentrated on strategies involving medical education programs on biosimilars., Competing Interests: Heloísa M. ResendeConsulting or Advisory Role: Libbs PharmaceuticalSpeakers' Bureau: Libbs PharmaceuticalTravel, Accommodations, Expenses: Pfizer Leandro LadislauEmployment: Daiichi Sankyo/Brazil (current), Libbs Pharmaceutical (previous) Ana Carolina F. CardosoEmployment: Libbs Pharmaceutical Juliana Dinéia P. BrandãoEmployment: Libbs Pharmaceutical Vivienne CastilhoEmployment: Libbs PharmaceuticalNo other potential conflicts of interest were reported.

Despite the implementation of social and health policies that positively affected the health of the populations in Brazil, since 2009 the country has experienced a slower decline of infant mortality. After an economic and political crisis, Brazil witnessed increases in infant mortality that raised questions about what are the determinants of infant mortality after the implementation of such policies. We conducted a scoping review to identify and summarize those determinants with searches in three databases: LILACS, MEDLINE, and SCIELO. We included studies published between 2010 and 2020. We selected 23 papers: 83% associated infant mortality with public policies; 78% related infant mortality with the use of the health system and socioeconomic and living conditions; and 27% related to individual characteristics to infant mortality. Inequalities in the access to healthcare seem to have important implications in reducing infant mortality. Socioeconomic conditions and health-related factors such as income, education, fertility, housing, and the Bolsa Família . Program coverage was pointed out as the main determinants of infant mortality. Likewise, recent changes in infant mortality in Brazil are likely related to these factors. We also identified a gap in terms of studies on a possible association between employment and infant mortality.

In recent years, significant work has been done in technological enhancements for mobility aids (smart walkers). However, most of this work does not cover the millions of people who have both mobility and visual impairments. In this paper, we design and study four different configurations of smart walkers that are specifically targeted to the needs of this population. We investigated different sensing technologies (ultrasound-based, infrared depth cameras and RGB cameras with advanced computer vision processing), software configurations, and user interface modalities (haptic and audio signal based). Our experiments show that there are several engineering choices that can be used in the design of such assistive devices. Furthermore, we found that a holistic evaluation of the end-to-end performance of the systems is necessary, as the quality of the user interface often has a larger impact on the overall performance than increases in the sensing accuracy beyond a certain point.

Background: Despite the implementation of a set of social and health policies, Brazil has experienced a slowdown in the decline of infant mortality, regional disparities and persistent high death levels, raising questions about the determinants of infant mortality after the implementation of these policies. The objective of this article is to propose a methodological approach aiming at identifying the determinants of infant mortality in Brazil after the implementation of those policies., Method: A series of multilevel panel data with fixed effect nested within-clusters were conducted supported by the concept of health capabilities based on data from 26 Brazilian states between 2004 and 2015. The dependent variables were the neonatal, the infant and the under-five mortality rates. The independent variables were the employment rate, per capita income, Bolsa Família Program coverage, the fertility rate, educational attainment, the number of live births by prenatal visits, the number of health professionals per thousand inhabitants, and the access to water supply and sewage services. We also used different time lags of employment rate to identify the impact of employment on the infant mortality rates over time, and household income stratified by minimum wages to analyze their effects on these rates., Results: The results showed that in addition to variables associated with infant mortality in previous studies, such as Bolsa Família Program, per capita income and fertility rate, other factors affect child mortality. Educational attainment, quality of prenatal care and access to health professionals are also elements impacting infant deaths. The results also identified an association between employment rate and different infant mortality rates, with employment impacting neonatal mortality up to 3 years and that a family income below 2 minimum wages increases the odds of infant deaths., Conclusion: The results proved that the methodology proposed allowed the use of variables based on aggregated data that could hardly be used by other methodologies.

Studying thermal stability of proteins not only provides insight into protein structure but also is instrumental in identifying previously unknown interaction partners. We develop a machine learning strategy that combines orthogonal partial least squares regression and stability screening of Silver Bullets Bio library to identify biologically active molecules that enhance protein stability. This strategy proves effective in extracting the stability-enhancing molecules for SMYD5, a histone lysine methyltransferase that regulates chromosome integrity. Protamine, a histone substitute in chromatin condensation during spermatogenesis, is identified as the most influential molecule to enhance SMYD5 thermal stability. We find that the C-terminal poly-glutamic acid tract (poly-E) and a 30-residue insertion in MYND domain (M-insertion), which are unique to SMYD5, regulate the structural stability. However, protamine plays a dominant role in SMYD5 stability, and in the presence of protamine, the poly-E tract or M-insertion loses its ability to affect the stability. The stability-enhancing effect of protamine is SMYD5 specific, and for SMYD2, a closely related homolog, protamine exhibits opposite, destabilizing effects. We find that both SMYD5 and SMYD2 interact with protamine, where SMYD5 interaction is independent of the poly-E tract and M-insertion. Protamine not only helps provide insight into the structure-stability relationships of SMYD5, but also suggests a potential functional link of SMYD5 to spermatogenesis. SMYD5 is a ubiquitously expressed gene with the highest expression in testis, especially in the seminiferous ducts that contain germ cells. Thus, our study opens up avenues that could help delineate major mechanisms underlying chromatin dynamics during spermatogenesis., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier Inc. All rights reserved.)