Search

Your search keyword '"Lacroix, Romaric"' showing total 310 results
Start Over Author "Lacroix, Romaric"
310 results on '"Lacroix, Romaric"'

3. Comparison of assays measuring extracellular vesicle tissue factor in plasma samples: communication from the ISTH SSC Subcommittee on Vascular Biology

Author

Bonifay, Amandine, Mackman, Nigel, Hisada, Yohei, Sachetto, Ana Teresa Azevedo, Hau, Chi, Gray, Elaine, Hogwood, John, Aharon, Anat, Badimon, Lina, Barile, Lucio, Baudar, Justine, Beckmann, Lennart, Benedikter, Birke, Bolis, Sara, Bouriche, Tarik, Brambilla, Marta, Burrello, Jacopo, Camera, Marina, Campello, Elena, Ettelaie, Camille, Faille, Dorothée, Featherby, Sophie, Franco, Corentin, Guldenpfennig, Maite, Hansen, John-Bjarne, Judicone, Coralie, Kim, Yohan, Kristensen, Soren Risom, Laakmann, Katrin, Langer, Florian, Latysheva, Nadezhda, Lucien, Fabrice, de Menezes, Erika Marques, Mullier, François, Norris, Philip, Nybo, Jette, Orbe, Josune, Osterud, Bjarne, Paramo, Jose A., Radu, Claudia M., Roncal, Carmen, Samadi, Nazanin, Snir, Omri, Suades, Rosa, Wahlund, Casper, Chareyre, Corinne, Abdili, Evelyne, Martinod, Kimberly, Thaler, Johannes, Dignat-George, Françoise, Nieuwland, Rienk, and Lacroix, Romaric

Published
2024
Full Text
View/download PDF

7. Ultra-lung-protective ventilation and biotrauma in severe ARDS patients on veno-venous extracorporeal membrane oxygenation: a randomized controlled study

Author

Guervilly, Christophe, Fournier, Théotime, Chommeloux, Juliette, Arnaud, Laurent, Pinglis, Camille, Baumstarck, Karine, Boucekine, Mohamed, Valera, Sabine, Sanz, Celine, Adda, Mélanie, Bobot, Mickaël, Daviet, Florence, Gragueb-Chatti, Ines, Forel, Jean-Marie, Roch, Antoine, Hraiech, Sami, Dignat-George, Françoise, Schmidt, Matthieu, Lacroix, Romaric, and Papazian, Laurent

Published
2022
Full Text
View/download PDF

14. How should we diagnose and treat blastic plasmacytoid dendritic cell neoplasm patients?

Author

Garnache-Ottou, Francine, Vidal, Chrystelle, Biichlé, Sabeha, Renosi, Florian, Poret, Eve, Pagadoy, Maïder, Desmarets, Maxime, Roggy, Anne, Seilles, Estelle, Soret, Lou, Schillinger, Françoise, Puyraimond, Sandrine, Petrella, Tony, Preudhomme, Claude, Roumier, Christophe, MacIntyre, Elisabeth A., Harrivel, Véronique, Desbrosses, Yohan, Gruson, Bérengère, Geneviève, Franck, Thepot, Sylvain, Drebit, Yuriy, Leguay, Thibaut, Gros, François-Xavier, Lechevalier, Nicolas, Saussoy, Pascale, Salaun, Véronique, Cornet, Edouard, Benseddik, Zehaira, Veyrat-Masson, Richard, Wagner-Ballon, Orianne, Salanoubat, Célia, Maynadié, Marc, Guy, Julien, Caillot, Denis, Jacob, Marie-Christine, Cahn, Jean-Yves, Gressin, Rémy, Rose, Johann, Quesnel, Bruno, Guerin, Estelle, Trimoreau, Franck, Feuillard, Jean, Gourin, Marie-Pierre, Plesa, Adriana, Baseggio, Lucile, Arnoux, Isabelle, Vey, Norbert, Blaise, Didier, Lacroix, Romaric, Arnoulet, Christine, Benet, Blandine, Dorvaux, Véronique, Bret, Caroline, Drenou, Bernard, Debliquis, Agathe, Latger-Cannard, Véronique, Bonmati, Caroline, Bene, Marie-Christine, Peterlin, Pierre, Ticchioni, Michel, Rohrlich, Pierre-Simon, Arnaud, Anne, Wickenhauser, Stefan, Bardet, Valérie, Brechignac, Sabine, Papoular, Benjamin, Raggueneau, Victoria, Vargaftig, Jacques, Letestu, Rémi, Lusina, Daniel, Braun, Thorsten, Foissaud, Vincent, Tamburini, Jérôme, Bennani, Hind, Freynet, Nicolas, Cordonnier, Catherine, Le Garff-Tavernier, Magali, Jacques, Nathalie, Maloum, Karim, Roos-Weil, Damien, Bouscary, Didier, Asnafi, Vahid, Lhermitte, Ludovic, Suarez, Felipe, Lengline, Etienne, Féger, Frédéric, Battipaglia, Giorgia, Mohty, Mohamad, Bouyer, Sabrina, Ghoual, Ouda, Dindinaud, Elodie, Basle, Caroline, Puyade, Mathieu, Lafon, Carinne, Fest, Thierry, Roussel, Mikael, Cahu, Xavier, Bera, Elsa, Daliphard, Sylvie, Jardin, Fabrice, Campos, Lydia, Solly, Françoise, Guyotat, Denis, Galoisy, Anne-Cécile, Eischen, Alice, Mayeur-Rousse, Caroline, Guffroy, Blandine, Recher, Christian, Loosveld, Marie, Garnier, Alice, Barlogis, Vincent, Rosenthal, Maria Alessandra, Brun, Sophie, Contentin, Nathalie, Maury, Sébastien, Callanan, Mary, Lefebvre, Christine, Maillard, Natacha, Okamba, Patricia, Ferrand, Christophe, Adotevi, Olivier, Saas, Philippe, Angelot-Delettre, Fanny, Binda, Delphine, and Deconinck, Eric

Published
2019
Full Text
View/download PDF

16. Microvesicles Are Associated with Early Veno Venous ECMO Circuit Change during Severe ARDS: A Prospective Observational Pilot Study

Author

Guervilly, Christophe, primary, Bousquet, Giovanni, additional, Arnaud, Laurent, additional, Gragueb-Chatti, Ines, additional, Daviet, Florence, additional, Adda, Mélanie, additional, Forel, Jean-Marie, additional, Dignat-George, Françoise, additional, Papazian, Laurent, additional, Roch, Antoine, additional, Lacroix, Romaric, additional, and Hraiech, Sami, additional

Published
2023
Full Text
View/download PDF

17. Macrophage IL-1β-positive microvesicles exhibit thrombo-inflammatory properties and are detectable in patients with active juvenile idiopathic arthritis

Author

Cambon, Audrey, primary, Rebelle, Charlotte, additional, Bachelier, Richard, additional, Arnaud, Laurent, additional, Robert, Stéphane, additional, Lagarde, Marie, additional, Muller, Romain, additional, Tellier, Edwige, additional, Kara, Yéter, additional, Leroyer, Aurélie, additional, Farnarier, Catherine, additional, Vallier, Loris, additional, Chareyre, Corinne, additional, Retornaz, Karine, additional, Jurquet, Anne-Laure, additional, Tran, Tu-Anh, additional, Lacroix, Romaric, additional, Dignat-George, Françoise, additional, and Kaplanski, Gilles, additional

Published
2023
Full Text
View/download PDF

18. Platelet-Derived Microparticles

Author

Cointe, Sylvie, Lacroix, Romaric, Dignat-George, Françoise, Gresele, Paolo, editor, Kleiman, Neal S., editor, Lopez, José A., editor, and Page, Clive P., editor

Published
2017
Full Text
View/download PDF

20. Clinical, biological, electrophysiological and therapeutic profile of patients with anti-MAG neuropathy according to MYD88L265P and CXCR4 mutations and underlying haemopathy.

Author

Guérémy, Alexandre, Boucraut, José, Boudjarane, John, Grapperon, Aude-Marie, Fortanier, Etienne, Farnault, Laure, Gabert, Jean, Vely, Frédéric, Lacroix, Romaric, Kouton, Ludivine, Attarian, Shahram, and Delmont, Emilien

Subjects
BRUTON tyrosine kinase, CXCR4 receptors, SOMATIC mutation, MUCOSA-associated lymphoid tissue lymphoma, ELECTROPHYSIOLOGY
Abstract

Introduction: Anti-MAG neuropathies are associated with an IgM monoclonal gammopathy of undetermined significance (MGUS) or with a malignant haemopathy. Our objective was to determine whether the presence of a haemopathy or somatic mutations of MYD88 and CXCR4 genes influences disease presentation and response to rituximab (RTX). Methods: We included 79 patients (mean age 74 years, disease duration 9.68 years) who had a bone marrow aspiration with morphologic and immunophenotypic analysis. MYD88L265P and CXCR4 mutations were analysed in peripheral B cells. Information collected included: inflammatory neuropathy cause and treatment sensory sum score (ISS), MRC testing, overall neuropathy limitation scale (ONLS), Rash-built Overall Disability Score (RODS), ataxia score, anti-MAG titres, peak IgM dosage, neurofilament light chain levels, motor and sensory amplitudes, motor unit index (MUNIX) and motor unit size index (MUSIX) sum scores. Efficacy of RTX was evaluated at 12 months in 26 patients. Results: Malignant haematological disorders were discovered in 17 patients (22%): 13 Waldenstrom macroglobulinemia, 3 marginal zone lymphoma and one mantle cell lymphoma. MYD88L265P mutation was detected in 29/60 (48%) patients and CXCR4 in 1 single patient. Disease severity, biological and electrophysiological data and response to RTX were comparable in patients with MGUS/lymphoma and patients with/without MYD88L265P mutation. ISS was lower and MUSIX higher in patients improved by RTX. Conclusions: MYD88L265P mutation and underlying haemopathies are not predictive of a more severe disease. However, in cases of resistant and progressive neuropathy, they provide an opportunity to prescribe newly available drugs such as Bruton tyrosine kinase inhibitors. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

22. A compendium of single extracellular vesicle flow cytometry

Author

Welsh, Joshua A, Arkesteijn, Ger J A, Bremer, Michel, Cimorelli, Michael, Dignat-George, Françoise, Giebel, Bernd, Görgens, André, Hendrix, An, Kuiper, Martine, Lacroix, Romaric, Lannigan, Joanne, van Leeuwen, Ton G, Lozano-Andrés, Estefanía, Rao, Shoaib, Robert, Stéphane, de Rond, Leonie, Tang, Vera A, Tertel, Tobias, Yan, Xiaomei, Wauben, Marca H M, Nolan, John P, Jones, Jennifer C, Nieuwland, Rienk, van der Pol, Edwin, Welsh, Joshua A, Arkesteijn, Ger J A, Bremer, Michel, Cimorelli, Michael, Dignat-George, Françoise, Giebel, Bernd, Görgens, André, Hendrix, An, Kuiper, Martine, Lacroix, Romaric, Lannigan, Joanne, van Leeuwen, Ton G, Lozano-Andrés, Estefanía, Rao, Shoaib, Robert, Stéphane, de Rond, Leonie, Tang, Vera A, Tertel, Tobias, Yan, Xiaomei, Wauben, Marca H M, Nolan, John P, Jones, Jennifer C, Nieuwland, Rienk, and van der Pol, Edwin

Abstract

Flow cytometry (FCM) offers a multiparametric technology capable of characterizing single extracellular vesicles (EVs). However, most flow cytometers are designed to detect cells, which are larger than EVs. Whereas cells exceed the background noise, signals originating from EVs partly overlap with the background noise, thereby making EVs more difficult to detect than cells. This technical mismatch together with complexity of EV-containing fluids causes limitations and challenges with conducting, interpreting and reproducing EV FCM experiments. To address and overcome these challenges, researchers from the International Society for Extracellular Vesicles (ISEV), International Society for Advancement of Cytometry (ISAC), and the International Society on Thrombosis and Haemostasis (ISTH) joined forces and initiated the EV FCM working group. To improve the interpretation, reporting, and reproducibility of future EV FCM data, the EV FCM working group published an ISEV position manuscript outlining a framework of minimum information that should be reported about an FCM experiment on single EVs (MIFlowCyt-EV). However, the framework contains limited background information. Therefore, the goal of this compendium is to provide the background information necessary to design and conduct reproducible EV FCM experiments. This compendium contains background information on EVs, the interaction between light and EVs, FCM hardware, experimental design and preanalytical procedures, sample preparation, assay controls, instrument data acquisition and calibration, EV characterization, and data reporting. Although this compendium focuses on EVs, many concepts and explanations could also be applied to FCM detection of other particles within the EV size range, such as bacteria, lipoprotein particles, milk fat globules, and viruses.

Published
2023

23. Methods for the identification and characterization of extracellular vesicles in cardiovascular studies - from exosomes to microvesicles

Author

Davidson, Sean M, Boulanger, Chantal M, Aikawa, Elena, Badimon, Lina, Barile, Lucio, Binder, Christoph J, Brisson, Alain, Buzas, Edit, Emanueli, Costanza, Jansen, Felix, Katsur, Miroslava, Lacroix, Romaric, Lim, Sai Kiang, Mackman, Nigel, Mayr, Manuel, Menasché, Philippe, Nieuwland, Rienk, Sahoo, Susmita, Takov, Kaloyan, Thum, Thomas, Vader, Pieter, Wauben, Marca H M, Witwer, Kenneth, Sluijter, Joost P G, Davidson, Sean M, Boulanger, Chantal M, Aikawa, Elena, Badimon, Lina, Barile, Lucio, Binder, Christoph J, Brisson, Alain, Buzas, Edit, Emanueli, Costanza, Jansen, Felix, Katsur, Miroslava, Lacroix, Romaric, Lim, Sai Kiang, Mackman, Nigel, Mayr, Manuel, Menasché, Philippe, Nieuwland, Rienk, Sahoo, Susmita, Takov, Kaloyan, Thum, Thomas, Vader, Pieter, Wauben, Marca H M, Witwer, Kenneth, and Sluijter, Joost P G

Abstract

Extracellular vesicles (EVs) are nanosized vesicles with a lipid bilayer that are released from cells of the cardiovascular system, and are considered important mediators of intercellular and extracellular communications. Two types of EVs of particular interest are exosomes and microvesicles, which have been identified in all tissue and body fluids and carry a variety of molecules including RNAs, proteins, and lipids. EVs have potential for use in the diagnosis and prognosis of cardiovascular diseases and as new therapeutic agents, particularly in the setting of myocardial infarction and heart failure. Despite their promise, technical challenges related to their small size make it challenging to accurately identify and characterize them, and to study EV-mediated processes. Here, we aim to provide the reader with an overview of the techniques and technologies available for the separation and characterization of EVs from different sources. Methods for determining the protein, RNA, and lipid content of EVs are discussed. The aim of this document is to provide guidance on critical methodological issues and highlight key points for consideration for the investigation of EVs in cardiovascular studies.

Published
2023

24. A compendium of single extracellular vesicle flow cytometry

Author

IOV Facs, Celbiologie, Welsh, Joshua A, Arkesteijn, Ger J A, Bremer, Michel, Cimorelli, Michael, Dignat-George, Françoise, Giebel, Bernd, Görgens, André, Hendrix, An, Kuiper, Martine, Lacroix, Romaric, Lannigan, Joanne, van Leeuwen, Ton G, Lozano-Andrés, Estefanía, Rao, Shoaib, Robert, Stéphane, de Rond, Leonie, Tang, Vera A, Tertel, Tobias, Yan, Xiaomei, Wauben, Marca H M, Nolan, John P, Jones, Jennifer C, Nieuwland, Rienk, van der Pol, Edwin, IOV Facs, Celbiologie, Welsh, Joshua A, Arkesteijn, Ger J A, Bremer, Michel, Cimorelli, Michael, Dignat-George, Françoise, Giebel, Bernd, Görgens, André, Hendrix, An, Kuiper, Martine, Lacroix, Romaric, Lannigan, Joanne, van Leeuwen, Ton G, Lozano-Andrés, Estefanía, Rao, Shoaib, Robert, Stéphane, de Rond, Leonie, Tang, Vera A, Tertel, Tobias, Yan, Xiaomei, Wauben, Marca H M, Nolan, John P, Jones, Jennifer C, Nieuwland, Rienk, and van der Pol, Edwin

Published
2023

25. Blood coagulation and beyond:Position paper from the Fourth Maastricht Consensus Conference on Thrombosis

Author

Akbulut, Asim Cengiz, Arisz, Ryanne A., Baaten, Constance C.F.M.J., Baidildinova, Gaukhar, Barakzie, Aarazo, Bauersachs, Rupert, Berg, Jur ten, van den Broek, Wout W.A., Boer, H. C.de, Bonifay, Amandine, Bröker, Vanessa, Buka, Richard J., Cate, Hugo ten, Cate-Hoek, Arina J.ten, Cointe, S., Luca, C. De, Simone, Ilaria De, Diaz, Rocio Vacik, Dignat-George, Françoise, Freson, Kathleen, Gazzaniga, Giulia, van Gorp, Eric C.M., Habibi, Anxhela, Henskens, Yvonne M.C., Iding, Aaron F.J., Khan, Abdullah, Koenderink, Gijsje H., Konkoth, Akhil, Lacroix, Romaric, Lahiri, Trisha, Lam, Wilbur, Lamerton, Rachel E., Lorusso, Roberto, Luo, Qi, Maas, Coen, Mccarty, Owen J.T., van der Meijden, Paola E.J., Meijers, Joost C.M., Mohapatra, Adarsh K., Nevo, Neta, Robles, Alejandro Pallares, Poncelet, Philippe, Reinhardt, Christoph, Ruf, Wolfram, Saraswat, Ronald, Schönichen, Claudia, Schutgens, Roger, Simioni, Paolo, Spada, Stefano, Spronk, Henri M.H., Tazhibayeva, Karlygash, Thachil, Jecko, Vallier, L., Veninga, Alicia, Verhamme, Peter, Visser, Chantal, Watson, Steve P., Wenzel, Philip, Willems, Ruth A.L., Willers, Anne, Zhang, Pengyu, Zifkos, Konstantinos, Zonneveld, Anton Jan van, Akbulut, Asim Cengiz, Arisz, Ryanne A., Baaten, Constance C.F.M.J., Baidildinova, Gaukhar, Barakzie, Aarazo, Bauersachs, Rupert, Berg, Jur ten, van den Broek, Wout W.A., Boer, H. C.de, Bonifay, Amandine, Bröker, Vanessa, Buka, Richard J., Cate, Hugo ten, Cate-Hoek, Arina J.ten, Cointe, S., Luca, C. De, Simone, Ilaria De, Diaz, Rocio Vacik, Dignat-George, Françoise, Freson, Kathleen, Gazzaniga, Giulia, van Gorp, Eric C.M., Habibi, Anxhela, Henskens, Yvonne M.C., Iding, Aaron F.J., Khan, Abdullah, Koenderink, Gijsje H., Konkoth, Akhil, Lacroix, Romaric, Lahiri, Trisha, Lam, Wilbur, Lamerton, Rachel E., Lorusso, Roberto, Luo, Qi, Maas, Coen, Mccarty, Owen J.T., van der Meijden, Paola E.J., Meijers, Joost C.M., Mohapatra, Adarsh K., Nevo, Neta, Robles, Alejandro Pallares, Poncelet, Philippe, Reinhardt, Christoph, Ruf, Wolfram, Saraswat, Ronald, Schönichen, Claudia, Schutgens, Roger, Simioni, Paolo, Spada, Stefano, Spronk, Henri M.H., Tazhibayeva, Karlygash, Thachil, Jecko, Vallier, L., Veninga, Alicia, Verhamme, Peter, Visser, Chantal, Watson, Steve P., Wenzel, Philip, Willems, Ruth A.L., Willers, Anne, Zhang, Pengyu, Zifkos, Konstantinos, and Zonneveld, Anton Jan van

Abstract

The 4th Maastricht Consensus Conference on Thrombosis (MCCT), included the following themes: Theme 1: The coagulome as a critical driver of cardiovascular disease Blood coagulation proteins also play divergent roles in biology and pathophysiology, related to specific organs, including brain, heart, bone marrow and kidney. Four investigators shared their views on these organ-specific topics. Theme 2: Novel mechanisms of thrombosis Mechanisms linking factor XII to fibrin, including their structural and physical properties, contribute to thrombosis, which is also affected by variation in microbiome status. Virus infections associated-coagulopathies perturb the hemostatic balance resulting in thrombosis and/or bleeding. Theme 3: How to limit bleeding risks: insights from translational studies This theme included state of the art methodology for exploring the contribution of genetic determinants of a bleeding diathesis; determination of polymorphisms in genes that control the rate of metabolism by the liver of P2Y12 inhibitors, to improve safety of antithrombotic therapy. Novel reversal agents for direct oral anticoagulants are discussed. Theme 4: Hemostasis in extracorporeal systems: how to utilize ex vivo models? Perfusion flow chamber and nanotechnology developments are developed for studying bleeding and thrombosis tendencies. Vascularised organoids are utilized for disease modeling and drug development studies. Strategies for tackling extracorporeal membrane oxygenation (ECMO) associated coagulopathy are discussed. Theme 5: Clinical dilemmas in thrombosis and antithrombotic management Plenary presentations addressed controversial areas, ie thrombophilia testing, thrombosis risk assessment in hemophilia, novel antiplatelet strategies and clinically tested factor XI(a) inhibitors,both possibly with reduced bleeding risk. Finally, Covid-19 associated coagulopathy is revisited.

Published
2023

26. Methods for the identification and characterization of extracellular vesicles in cardiovascular studies - from exosomes to microvesicles

Author

Sub General Pharmaceutics, Celbiologie, dB&C I&I, Cell Biology, Metabolism & Cancer - Cellbiology, Davidson, Sean M, Boulanger, Chantal M, Aikawa, Elena, Badimon, Lina, Barile, Lucio, Binder, Christoph J, Brisson, Alain, Buzas, Edit, Emanueli, Costanza, Jansen, Felix, Katsur, Miroslava, Lacroix, Romaric, Lim, Sai Kiang, Mackman, Nigel, Mayr, Manuel, Menasché, Philippe, Nieuwland, Rienk, Sahoo, Susmita, Takov, Kaloyan, Thum, Thomas, Vader, Pieter, Wauben, Marca H M, Witwer, Kenneth, Sluijter, Joost P G, Sub General Pharmaceutics, Celbiologie, dB&C I&I, Cell Biology, Metabolism & Cancer - Cellbiology, Davidson, Sean M, Boulanger, Chantal M, Aikawa, Elena, Badimon, Lina, Barile, Lucio, Binder, Christoph J, Brisson, Alain, Buzas, Edit, Emanueli, Costanza, Jansen, Felix, Katsur, Miroslava, Lacroix, Romaric, Lim, Sai Kiang, Mackman, Nigel, Mayr, Manuel, Menasché, Philippe, Nieuwland, Rienk, Sahoo, Susmita, Takov, Kaloyan, Thum, Thomas, Vader, Pieter, Wauben, Marca H M, Witwer, Kenneth, and Sluijter, Joost P G

Published
2023

27. Methods for the identification and characterization of extracellular vesicles in cardiovascular studies: from exosomes to microvesicles

Author

CDL Research analisten, Circulatory Health, Experimental Cardiology Laboratory, Onderzoek Cardiovasculair Reg. Med., Regenerative Medicine and Stem Cells, Davidson, Sean M., Boulanger, Chantal M., Aikawa, Elena, Badimon, Lina, Barile, Lucio, Binder, Christoph J., Brisson, Alain, Buzas, Edit, Emanueli, Costanza, Jansen, Felix, Katsur, Miroslava, Lacroix, Romaric, Lim, Sai Kiang, Mackman, Nigel, Mayr, Manuel, Menasché, Philippe, Nieuwland, Rienk, Sahoo, Susmita, Takov, Kaloyan, Thum, Thomas, Vader, Pieter, Wauben, Marca H.M., Witwer, Kenneth, Sluijter, Joost P.G., CDL Research analisten, Circulatory Health, Experimental Cardiology Laboratory, Onderzoek Cardiovasculair Reg. Med., Regenerative Medicine and Stem Cells, Davidson, Sean M., Boulanger, Chantal M., Aikawa, Elena, Badimon, Lina, Barile, Lucio, Binder, Christoph J., Brisson, Alain, Buzas, Edit, Emanueli, Costanza, Jansen, Felix, Katsur, Miroslava, Lacroix, Romaric, Lim, Sai Kiang, Mackman, Nigel, Mayr, Manuel, Menasché, Philippe, Nieuwland, Rienk, Sahoo, Susmita, Takov, Kaloyan, Thum, Thomas, Vader, Pieter, Wauben, Marca H.M., Witwer, Kenneth, and Sluijter, Joost P.G.

Published
2023

28. Blood coagulation and beyond: Position paper from the Fourth Maastricht Consensus Conference on Thrombosis

Author

CDL Cluster Onderzoek en Onderwijs, Circulatory Health, Poli Van Creveldkliniek Medisch, Child Health, Infection & Immunity, Akbalut, Cengiz, Arisz, Ryanne, Baaten, Constance, Baildildinova, Gaukhar, Barakzie, Aarazo, Bauersachs, Rupert, Ten Berg, Jurriën M, van den Broek, Wout, de Boer, Hetty C, Broker, Vanessa, Buka, Richard, Ten Cate, Hugo, Cate, Arina Ten, De Luca, Ciro, De Simone, Ilaria, Dignat-George, Françoise, Freson, Kathleen, Gazzaniga, Giulia, van Gorp, Eric, Habibi, Anxhela, Henskens, Yvonne M C, Iding, Aaron F J, Khan, Abdullah, Koenderink, Gijsje, Konkoth, Akhil, Lacroix, Romaric, Lahiri, Trisha, Lam, Wilbur, Lamerton, Rachel, Lorusso, Roberto, Luo, Qi, Maas, Coen, McCarty, Owen J T, van der Meijden, Paola, Meijers, Joost, Mohapatra, Adarsh, Nevo, Neta, Pallares Robles, Alejandro, Poncelet, Philippe, Reinhardt, Christoph, Ruf, Wolfram, Saraswat, Ronald, Schonichen, Claudia, Schutgens, Roger E G, Simioni, Paolo, Spada, Stefano, Spronk, Henri M H, Tazhibayeva, Karlygash, Thachil, Jecko, Vacik-Diaz, Rocio, Veninga, Alicia, Verhamme, Peter, Visser, Chantal, Watson, Steve P, Wenzel, Philip, Willems, Ruth, Willers, Anne, Zhang, Pengyu, Zifkos, Konstantinos, van Zonneveld, Anton-Jan, CDL Cluster Onderzoek en Onderwijs, Circulatory Health, Poli Van Creveldkliniek Medisch, Child Health, Infection & Immunity, Akbalut, Cengiz, Arisz, Ryanne, Baaten, Constance, Baildildinova, Gaukhar, Barakzie, Aarazo, Bauersachs, Rupert, Ten Berg, Jurriën M, van den Broek, Wout, de Boer, Hetty C, Broker, Vanessa, Buka, Richard, Ten Cate, Hugo, Cate, Arina Ten, De Luca, Ciro, De Simone, Ilaria, Dignat-George, Françoise, Freson, Kathleen, Gazzaniga, Giulia, van Gorp, Eric, Habibi, Anxhela, Henskens, Yvonne M C, Iding, Aaron F J, Khan, Abdullah, Koenderink, Gijsje, Konkoth, Akhil, Lacroix, Romaric, Lahiri, Trisha, Lam, Wilbur, Lamerton, Rachel, Lorusso, Roberto, Luo, Qi, Maas, Coen, McCarty, Owen J T, van der Meijden, Paola, Meijers, Joost, Mohapatra, Adarsh, Nevo, Neta, Pallares Robles, Alejandro, Poncelet, Philippe, Reinhardt, Christoph, Ruf, Wolfram, Saraswat, Ronald, Schonichen, Claudia, Schutgens, Roger E G, Simioni, Paolo, Spada, Stefano, Spronk, Henri M H, Tazhibayeva, Karlygash, Thachil, Jecko, Vacik-Diaz, Rocio, Veninga, Alicia, Verhamme, Peter, Visser, Chantal, Watson, Steve P, Wenzel, Philip, Willems, Ruth, Willers, Anne, Zhang, Pengyu, Zifkos, Konstantinos, and van Zonneveld, Anton-Jan

Published
2023

30. Macrophage IL-1b-positive microvesicles exhibit thromboinflammatory properties and are detectable in patients with active juvenile idiopathic arthritis.

Author

Cambon, Audrey, Rebelle, Charlotte, Bachelier, Richard, Arnaud, Laurent, Robert, Stéphane, Lagarde, Marie, Muller, Romain, Tellier, Edwige, Kara, Yéter, Leroyer, Aurélie, Farnarier, Catherine, Vallier, Loris, Chareyre, Corinne, Retornaz, Karine, Jurquet, Anne-Laure, Tran, Tu-Anh, Lacroix, Romaric, Dignat-George, Françoise, and Kaplanski, Gilles

Subjects
JUVENILE idiopathic arthritis, EXTRACELLULAR vesicles, INTERLEUKIN-1, MACROPHAGES, NLRP3 protein
Abstract

Objective: IL-1b is a leaderless cytokine with poorly known secretory mechanisms that is barely detectable in serum of patients, including those with an IL-1b-mediated disease such as systemic juvenile idiopathic arthritis (sJIA). Leukocyte microvesicles (MVs) may be a mechanism of IL-1b secretion. The first objective of our study was to characterize IL-1b-positive MVs obtained from macrophage cell culture supernatants and to investigate their biological functions in vitro and in vivo. The second objective was to detect circulating IL-1b-positive MVs in JIA patients. Methods: MVs were purified by serial centrifugations from PBMCs, or THP-1 differentiated into macrophages, then stimulated with LPS ± ATP. MV content was analyzed for the presence of IL-1b, NLRP3 inflammasome, caspase-1, P2X7 receptor, and tissue factor (TF) using ELISA, Western blot, or flow cytometry. MV biological properties were studied in vitro by measuring VCAM-1, ICAM-1, and Eselectin expression after HUVEC co-culture and factor-Xa generation test was realized. In vivo, MVs’ ability to recruit leukocytes in a murine model of peritonitis was evaluated. Plasmatic IL-1b-positive MVs were studied ex vivo in 10 active JIA patients using flow cytometry. Results: THP-1-derived macrophages stimulated with LPS and ATP released MVs, which contained NLRP3, caspase-1, and the 33-kDa precursor and 17-kDa mature forms of IL-1b and bioactive TF. IL-1b-positive MVs expressed P2X7 receptor and released soluble IL-1b in response to ATP stimulation in vitro. In mice, MVs induced a leukocyte peritoneal infiltrate, which was reduced by treatment with the IL-1 receptor antagonist. Finally, IL-1b-positive MVs were detectable in plasma from 10 active JIA patients. Conclusion: MVs shed from activated macrophages contain IL-1b, NLRP3 inflammasome components, and TF, and constitute thrombo-inflammatory vectors that can be detected in the plasma from active JIA patients. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

32. Blood Coagulation and Beyond: Position Paper from the Fourth Maastricht Consensus Conference on Thrombosis

Author

Akbulut, Asim Cengiz, additional, Arisz, Ryanne A., additional, Baaten, Constance C. F. M. J., additional, Baidildinova, Gaukhar, additional, Barakzie, Aarazo, additional, Bauersachs, Rupert, additional, ten Berg, Jur, additional, van den Broek, Wout W. A., additional, de Boer, H. C., additional, Bonifay, Amandine, additional, Bröker, Vanessa, additional, Buka, Richard J., additional, ten Cate, Hugo, additional, ten Cate-Hoek, Arina J., additional, Cointe, S., additional, De Luca, Ciro, additional, De Simone, Ilaria, additional, Diaz, Rocio Vacik, additional, Dignat-George, Françoise, additional, Freson, Kathleen, additional, Gazzaniga, Giulia, additional, van Gorp, Eric C. M., additional, Habibi, Anxhela, additional, Henskens, Yvonne M. C., additional, Iding, Aaron F. J., additional, Khan, Abdullah, additional, Koenderink, Gijsje H., additional, Konkoth, Akhil, additional, Lacroix, Romaric, additional, Lahiri, Trisha, additional, Lam, Wilbur, additional, Lamerton, Rachel E., additional, Lorusso, Roberto, additional, Luo, Qi, additional, Maas, Coen, additional, McCarty, Owen J. T., additional, van der Meijden, Paola E. J., additional, Meijers, Joost C. M., additional, Mohapatra, Adarsh K., additional, Nevo, Neta, additional, Robles, Alejandro Pallares, additional, Poncelet, Philippe, additional, Reinhardt, Christoph, additional, Ruf, Wolfram, additional, Saraswat, Ronald, additional, Schönichen, Claudia, additional, Schutgens, Roger, additional, Simioni, Paolo, additional, Spada, Stefano, additional, Spronk, Henri M. H., additional, Tazhibayeva, Karlygash, additional, Thachil, Jecko, additional, Vallier, L., additional, Veninga, Alicia, additional, Verhamme, Peter, additional, Visser, Chantal, additional, Watson, Steve P., additional, Wenzel, Philip, additional, Willems, Ruth A. L., additional, Willers, Anne, additional, Zhang, Pengyu, additional, Zifkos, Konstantinos, additional, and van Zonneveld, Anton Jan, additional

Published
2023
Full Text
View/download PDF

33. A compendium of single extracellular vesicle flow cytometry

Author

Welsh, Joshua A., primary, Arkesteijn, Ger J. A., additional, Bremer, Michel, additional, Cimorelli, Michael, additional, Dignat‐George, Françoise, additional, Giebel, Bernd, additional, Görgens, André, additional, Hendrix, An, additional, Kuiper, Martine, additional, Lacroix, Romaric, additional, Lannigan, Joanne, additional, van Leeuwen, Ton G., additional, Lozano‐Andrés, Estefanía, additional, Rao, Shoaib, additional, Robert, Stéphane, additional, de Rond, Leonie, additional, Tang, Vera A., additional, Tertel, Tobias, additional, Yan, Xiaomei, additional, Wauben, Marca H. M., additional, Nolan, John P., additional, Jones, Jennifer C., additional, Nieuwland, Rienk, additional, and van der Pol, Edwin, additional

Published
2023
Full Text
View/download PDF

34. A compendium of single extracellular vesicle flow cytometry

Author

Welsh, Joshua A., Arkesteijn, Ger J. A., Bremer, Michel, Cimorelli, Michael, Dignat-George, Françoise, Giebel, Bernd, Görgens, André, Hendrix, An, Kuiper, Martine, Lacroix, Romaric, Lannigan, Joanne, van Leeuwen, Ton G., Lozano-Andrés, Estefanía, Rao, Shoaib, Robert, Stéphane, de Rond, Leonie, Tang, Vera A., Tertel, Tobias, Yan, Xiaomei, Wauben, Marca H. M., Nolan, John P., Jones, Jennifer C., Nieuwland, Rienk, and van der Pol, Edwin

Subjects
Medizin
Abstract

Flow cytometry (FCM) offers a multiparametric technology capable of characterizing single extracellular vesicles (EVs). However, most flow cytometers are designed to detect cells, which are larger than EVs. Whereas cells exceed the background noise, signals originating from EVs partly overlap with the background noise, thereby making EVs more difficult to detect than cells. This technical mismatch together with complexity of EV-containing fluids causes limitations and challenges with conducting, interpreting and reproducing EV FCM experiments. To address and overcome these challenges, researchers from the International Society for Extracellular Vesicles (ISEV), International Society for Advancement of Cytometry (ISAC), and the International Society on Thrombosis and Haemostasis (ISTH) joined forces and initiated the EV FCM working group. To improve the interpretation, reporting, and reproducibility of future EV FCM data, the EV FCM working group published an ISEV position manuscript outlining a framework of minimum information that should be reported about an FCM experiment on single EVs (MIFlowCyt-EV). However, the framework contains limited background information. Therefore, the goal of this compendium is to provide the background information necessary to design and conduct reproducible EV FCM experiments. This compendium contains background information on EVs, the interaction between light and EVs, FCM hardware, experimental design and preanalytical procedures, sample preparation, assay controls, instrument data acquisition and calibration, EV characterization, and data reporting. Although this compendium focuses on EVs, many concepts and explanations could also be applied to FCM detection of other particles within the EV size range, such as bacteria, lipoprotein particles, milk fat globules, and viruses. CA extern

Published
2023

36. Plasma levels of E‐selectin are associated with retinopathy in sickle cell disease

Author

Agouti, Imane, primary, Masson, Elodie, additional, Loundou, Anderson, additional, Jean, Estelle, additional, Arnaud, Laurent, additional, Abdili, Evelyne, additional, Berenger, Patricia, additional, Lavoipierre, Virginie, additional, Séguier, Julie, additional, Dignat‐George, Françoise, additional, Lacroix, Romaric, additional, and Bernit, Emmanuelle, additional

Published
2022
Full Text
View/download PDF

37. Culture of human nasal olfactory stem cells and their extracellular vesicles as advanced therapy medicinal products

Author

Jaloux, Charlotte, primary, Bonnet, Maxime, additional, Vogtensperger, Marie, additional, Witters, Marie, additional, Veran, Julie, additional, Giraudo, Laurent, additional, Sabatier, Florence, additional, Michel, Justin, additional, Lacroix, Romaric, additional, Chareyre, Corinne, additional, Legré, Regis, additional, Guiraudie-Capraz, Gaelle, additional, and Féron, François, additional

Published
2022
Full Text
View/download PDF

39. Circulating microvesicles correlate with radiation proctitis complication after radiotherapy

Author

Ribault, Alexandre, Benadjaoud, Mohamed Amine, Squiban, Claire, Arnaud, Laurent, Judicone, Coralie, Leroyer, Aurélie, Rousseau, Alexandra, Huet, Christelle, Guha, Chandan, Benderitter, Marc, Lacroix, Romaric, Flamant, Stephane, Chen, Emily, Simon, Jean-Marc, Tamarat, Radia, Institut de Radioprotection et de Sûreté Nucléaire (IRSN), Hôpital de la Timone [CHU - APHM] (TIMONE), Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de Recherche Clinique de l’Est Parisien [CHU Saint-Antoine] (URC-EST), Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts (CHNO)-CHU Tenon [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-CHU Trousseau [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-CHU Rothschild [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Direction de la Recherche Clinique et de l'Innovation [AP-HP] (DRCI), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Albert Einstein College of Medicine [New York], Columbia University Medical Center (CUMC), Columbia University [New York], Service d'Oncologie Radiothérapie [CHU Pitié Salpétrière], CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Sorbonne Université (SU), INCa through PHRC AOM08324. EIC was supported by the National Institutes of Health (5P30CA013696-42, NIH/NCI and 2U19AI067773-11, NIH/NIAID), Cosenza, Bruno, Laboratoire de Radiobiologie des expositions médicales (IRSN/PSE-SANTE/SERAMED/LRMed), Service de recherche en radiobiologie et en médecine régénérative (IRSN/PSE-SANTE/SERAMED), Institut de Radioprotection et de Sûreté Nucléaire (IRSN)-Institut de Radioprotection et de Sûreté Nucléaire (IRSN), Centre d'investigation clinique Paris Est [CHU Pitié Salpêtrière] (CIC Paris-Est), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), Laboratoire de dosimétrie des rayonnements ionisants (IRSN/PSE-SANTE/SDOS/LDRI), Service de dosimétrie (IRSN/PSE-SANTE/SDOS), Pôle Santé Environnement - Direction Santé (IRSN/PSE-SANTE), Laboratoire de Radiobiologie des expositions accidentelles (IRSN/PSE-SANTE/SERAMED/LRAcc), and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)

Subjects
[SDV] Life Sciences [q-bio], Multidisciplinary, [SDV]Life Sciences [q-bio]
Abstract

In a large retrospective study, we assessed the putative use of circulating microvesicles (MVs), as innovative biomarkers of radiation toxicity in a cohort of 208 patients with prostate adenocarcinoma overexposed to radiation. The level of platelet (P)-, monocyte (M)- and endothelial (E)-derived MVs were assessed by flow cytometry. Rectal bleeding toxicity scores were collected at the time of blood sampling and during the routine follow-up and were tested for association with MVs using a multivariate logistic regression. MVs dosimetric correlation was investigated using dose volume histograms information available for a subset of 36 patients. The number of PMVs was significantly increased in patients with highest toxicity grades compared to lower grades. Risk prediction analysis revealed that increased numbers of PMVs, and an increased amount of MMVs relative to EMVs, were associated with worst rectal bleeding grade compared to the time of blood sampling. Moreover, a significant correlation was found between PMV and MMV numbers, with the range of doses up to the median exposure (40 Gy) of bladder/rectum and anterior rectal wall, respectively. MVs could be considered as new biomarkers to improve the identification of patients with high toxicity grade and may be instrumental for the prognosis of radiation therapy complications.

Published
2022
Full Text
View/download PDF

43. Circulating microvesicles correlate with radiation proctitis complication after radiotherapy

Author

Ribault, Alexandre, primary, Benadjaoud, Mohamed Amine, additional, Squiban, Claire, additional, Arnaud, Laurent, additional, Judicone, Coralie, additional, Leroyer, Aurélie S., additional, Rousseau, Alexandra, additional, Huet, Christelle, additional, Guha, Chandan, additional, Benderitter, Marc, additional, Lacroix, Romaric, additional, FLAMANT, Stephane, additional, Chen, Emily I., additional, Simon, Jean-Marc, additional, and Tamarat, Radia, additional

Published
2022
Full Text
View/download PDF

44. A new strategy to count and sort neutrophil‐derived extracellular vesicles: Validation in infectious disorders

Author

Bonifay, Amandine, primary, Robert, Stéphane, additional, Champagne, Belinda, additional, Petit, Paul‐Rémi, additional, Eugène, Aude, additional, Chareyre, Corinne, additional, Duchez, Anne‐Claire, additional, Vélier, Mélanie, additional, Fritz, Shirley, additional, Vallier, Loris, additional, Lacroix, Romaric, additional, and Dignat‐George, Françoise, additional

Published
2022
Full Text
View/download PDF

45. Methods for the identification and characterization of extracellular vesicles in cardiovascular studies: from exosomes to microvesicles

Author

Davidson, Sean M., primary, Boulanger, Chantal M., additional, Aikawa, Elena, additional, Badimon, Lina, additional, Barile, Lucio, additional, Binder, Christoph J., additional, Brisson, Alain, additional, Buzas, Edit, additional, Emanueli, Costanza, additional, Jansen, Felix, additional, Katsur, Miroslava, additional, Lacroix, Romaric, additional, Lim, Sai Kiang, additional, Mackman, Nigel, additional, Mayr, Manuel, additional, Menasché, Philippe, additional, Nieuwland, Rienk, additional, Sahoo, Susmita, additional, Takov, Kaloyan, additional, Thum, Thomas, additional, Vader, Pieter, additional, Wauben, Marca H.M., additional, Witwer, Kenneth, additional, and Sluijter, Joost P.G., additional

Published
2022
Full Text
View/download PDF

46. Tracking Radiolabeled Endothelial Microvesicles Predicts Their Therapeutic Efficacy: A Proof-of-Concept Study in Peripheral Ischemia Mouse Model Using SPECT/CT Imaging

Author

Giraud, Romain, primary, Moyon, Anaïs, additional, Simoncini, Stéphanie, additional, Duchez, Anne-Claire, additional, Nail, Vincent, additional, Chareyre, Corinne, additional, Bouhlel, Ahlem, additional, Balasse, Laure, additional, Fernandez, Samantha, additional, Vallier, Loris, additional, Hache, Guillaume, additional, Sabatier, Florence, additional, Dignat-George, Françoise, additional, Lacroix, Romaric, additional, Guillet, Benjamin, additional, and Garrigue, Philippe, additional

Published
2022
Full Text
View/download PDF

47. Biomarqueurs vésiculaires: Opportunités et défis dans les maladies cardiovasculaires et les cancers

Author

Bonifay, Amandine, Ghayad, Sandra, Lacroix, Romaric, Dignat-George, Francoise, Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Lebanese University [Beirut] (LU), Hôpital de la Conception [CHU - APHM] (LA CONCEPTION), Physiopathologie de l'Endothelium, Vascular research center of Marseille (VRCM), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU)

Subjects
[SDV]Life Sciences [q-bio]
Abstract

International audience; Les maladies cardiovasculaires et les cancers sont les premières causes de mortalité et de morbidité dans le monde. La recherche de biomarqueurs pertinents est un réel enjeu pour améliorer leur prise en charge clinique et thérapeutique. Les progrès rapides faits sur l’identification du contenu moléculaire et fonctionnel des vésicules extracellulaires et le développement de méthodologies sensibles et robustes identifient ces dernières comme des biomarqueurs prometteurs, accessibles dans les biopsies liquides. L’adaptation des étapes pré-analytiques, analytiques et une standardisation adaptée à chaque cible vésiculaire sont des prérequis indispensables pour accélérer le transfert de ces biomarqueurs vésiculaires vers la clinique et accompagner le développement d’une médecine personnalisée.

Published
2021
Full Text
View/download PDF

48. Plasma levels of E‐selectin are associated with retinopathy in sickle cell disease.

Author

Agouti, Imane, Masson, Elodie, Loundou, Anderson, Jean, Estelle, Arnaud, Laurent, Abdili, Evelyne, Berenger, Patricia, Lavoipierre, Virginie, Séguier, Julie, Dignat‐George, Françoise, Lacroix, Romaric, and Bernit, Emmanuelle

Subjects
SICKLE cell anemia, OCULAR injuries, VASCULAR endothelium, LEG ulcers, ENDOTHELIAL cells
Abstract

Background: The vascular endothelium is markedly disrupted in sickle cell disease (SCD) and is the converging cascade of the complex pathophysiologic processes linked to sickle cell vasculopathy. Circulating endothelial activation and/or apoptotic markers may reflect this endothelial activation/damage that contributes to the pathophysiology of the SCD vascular complications. Methods: Plasmatic levels of circulating endothelial cells (CECs), E‐selectin, progenitor's endothelial cells (EPCs), and circulating extracellular vesicles (EVs) were evaluated in 50 SCD patients, 16 with vasculopathy. The association between these markers and the occurrence of disease‐related microvascular injuries of the eye (retinopathy), kidney (nephropathy), and skin (chronic active ulcers) was explored. Results: Among the endothelial activation markers studied, only higher plasma levels of E‐selectin were found in SCD patients with vasculopathy (p =.015). Increased E‐selectin levels were associated with retinopathy (p <.001) but not with nephropathy or leg ulcers. All patients, at steady state, with or without vasculopathy, did not display a high count of CEC and EPC, markers of endothelial injury and repair. We did not show any significant differences in EVs levels between vasculopathy and not vasculopathy SCD patients. Conclusions: Further studies will be required to determine whether the E‐selectin could be used as an early biomarker of retinopathy sickle cell development. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

49. Rheopheresis Performed in Hemodialysis Patients Targets Endothelium and Has an Acute Anti-Inflammatory Effect.

Author

Solignac, Justine, Lacroix, Romaric, Arnaud, Laurent, Abdili, Evelyne, Bouchouareb, Dammar, Burtey, Stéphane, Brunet, Philippe, Dignat-George, Françoise, and Robert, Thomas

Subjects
*HEMODIALYSIS patients, *VASCULAR endothelial growth factors, *TISSUE adhesions, *ENDOTHELIUM, *PERIPHERAL vascular diseases
Abstract

Background: Rheopheresis is a double-filtration plasmapheresis that removes a defined spectrum of high-molecular-weight proteins to lower plasma viscosity and improves microcirculation disorders. This technique can be performed in hemodialysis (HD) patients with severe microischemia. Interestingly, some studies showed that rheopheresis sessions improve endothelial function. Methods: Our study evaluated the inflammatory and endothelial biomarker evolution in 23 HD patients treated or not with rheopheresis. A p value ≤ 0.001 was considered statistically significant. Results: Thirteen HD patients treated by rheopheresis either for a severe peripheral arterial disease (N = 8) or calciphylaxis (N = 5) were analyzed. Ten control HD patients were also included in order to avoid any misinterpretation of the rheopheresis effects in regard to the HD circuit. In the HD group without rheopheresis, the circulating endothelial adhesion molecules, cytokines, angiogenic factor concentrations, and circulating levels were not modified. In the HD group with rheopheresis, the circulating endothelial adhesion molecules (sVCAM-1, sP-selectin, and sE-selectin) experienced a significant reduction, except sICAM-1. Among the pro-inflammatory cytokines, TNF-α was significantly reduced by 32.6% [(−42.2)–(−22.5)] (p < 0.0001), while the anti-inflammatory cytokine IL-10 increased by 674% (306–1299) (p < 0.0001). Among the angiogenic factors, only sEndoglin experienced a significant reduction. The CEC level trended to increase from 13 (3–33) cells/mL to 43 (8–140) cells/mL (p = 0.002). We did not observe any difference on the pre-session values of the molecules of interest between the first rheopheresis session and the last rheopheresis session. Conclusion: Rheopheresis immediately modified the inflammation balance and the endothelial injury biomarkers. Further studies are needed to understand the mechanisms underlying these biological observations. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results