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17 results on '"Lachot, Sébastien"'

1. Tailored Digital PCR Follow-Up of Rare Fusion Transcripts after Initial Detection through RNA Sequencing in Hematological Malignancies

Author

Boulland, Marie-Laure, Aliouat, Amyra, Jalaber, Elie, Desmares, Anne, Toujani, Saloua, Luque Paz, Damien, Wiber, Margaux, Voirin, Emeline, Lachot, Sébastien, Basinko, Audrey, Lambert, Wayne-Corentin, Carras, Sylvain, Cousin, Elie, Marchand, Tony, de Tayrac, Marie, Fest, Thierry, Houot, Roch, and Pastoret, Cédric

Published
2024
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2. Modulation of bone marrow and peripheral blood cytokine levels by age and clonal hematopoiesis in healthy individuals

Author

Ravalet, Noémie, Guermouche, Hélène, Hirsch, Pierre, Picou, Frédéric, Foucault, Amélie, Gallay, Nathalie, Martignoles, Jean-Alain, Beaud, Jenny, Suner, Ludovic, Deswarte, Caroline, Lachot, Sébastien, Rault, Emmanuelle, Largeaud, Laëtitia, Gissot, Valérie, Béné, Marie-Christine, Gyan, Emmanuel, Delhommeau, François, and Herault, Olivier

Published
2023
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3. What is the impact of blood pressure on neurological symptoms and the risk of ESKD in primary and secondary thrombotic microangiopathies based on clinical presentation: a retrospective study

Author

Halimi, Jean-Michel, Thoreau, Benjamin, von Tokarski, Florent, Bauvois, Adeline, Gueguen, Juliette, Goin, Nicolas, Barbet, Christelle, Cloarec, Sylvie, Mérieau, Elodie, Lachot, Sébastien, Garot, Denis, Lemaignen, Adrien, Gyan, Emmanuel, Perrotin, Franck, Pouplard, Claire, Maillot, François, Gatault, Philippe, Sautenet, Bénédicte, Rusch, Emmanuel, Frémeaux-Bacchi, Véronique, Vigneau, Cécile, Bayer, Guillaume, and Fakhouri, Fadi

Published
2022
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6. Infection in Patients with Suspected Thrombotic Microangiopathy Based on Clinical Presentation

Author

Thoreau, Benjamin, primary, von Tokarski, Florent, additional, Bauvois, Adeline, additional, Bayer, Guillaume, additional, Barbet, Christelle, additional, Cloarec, Sylvie, additional, Mérieau, Elodie, additional, Lachot, Sébastien, additional, Garot, Denis, additional, Bernard, Louis, additional, Gyan, Emmanuel, additional, Perrotin, Franck, additional, Pouplard, Claire, additional, Maillot, François, additional, Gatault, Philippe, additional, Sautenet, Bénédicte, additional, Rusch, Emmanuel, additional, Frémeaux-Bacchi, Véronique, additional, Vigneau, Cécile, additional, Fakhouri, Fadi, additional, and Halimi, Jean-Michel, additional

Published
2021
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7. Automated Early Detection of Myelodysplastic Syndrome within the General Population Using the Research Parameters of Beckman–Coulter DxH 800 Hematology Analyzer

Author

Ravalet, Noémie, primary, Foucault, Amélie, additional, Picou, Frédéric, additional, Gombert, Martin, additional, Renoult, Emmanuel, additional, Lejeune, Julien, additional, Vallet, Nicolas, additional, Lachot, Sébastien, additional, Rault, Emmanuelle, additional, Gyan, Emmanuel, additional, Bene, Marie C., additional, and Herault, Olivier, additional

Published
2021
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9. Standardization of Flow Cytometric Immunophenotyping for Hematological Malignancies: The FranceFlow Group Experience.

Author

UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - (MGD) Département de pharmacie, Solly, Françoise, Angelot-Delettre, Fanny, Ticchioni, Michel, Geneviève, Franck, Rambaud, Hubert, Baseggio, Lucile, Plesa, Adriana, Debliquis, Agathe, Garnache-Ottou, Francine, Roggy, Anne, Campos, Lydia, Aanei, Carmen, Rosenthal-Allieri, Alessandra, Georget, Marie-Thérèse, Lachot, Sébastien, Jacob, Marie-Christine, Robillard, Nelly, Wuilleme, Soraya, Andre-Kerneis, Elisabeth, Cornet, Edouard, Salaun, Véronique, Bennami, Hind, Lhoumeau, Anne-Catherine, Arnoulet, Christine, JACQMIN, Hugues, NEYMAN, Nicolas, Latger-Cannard, Véronique, Massin, Fredéric, Lainey, Elodie, Le Garff-Tavernier, Magali, Costopoulos, Myrto, Roussel, Mickael, Mayeur-Rousse, Caroline, Eischen, Alice, Raggeneau, Victoria, Derrieux, Coralie, Maurer, Maxime, Asnafi, Vahid, Trinquand, Amélie, Brouzes, Chantal, Lhermitte, Ludovic, UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - (MGD) Département de pharmacie, Solly, Françoise, Angelot-Delettre, Fanny, Ticchioni, Michel, Geneviève, Franck, Rambaud, Hubert, Baseggio, Lucile, Plesa, Adriana, Debliquis, Agathe, Garnache-Ottou, Francine, Roggy, Anne, Campos, Lydia, Aanei, Carmen, Rosenthal-Allieri, Alessandra, Georget, Marie-Thérèse, Lachot, Sébastien, Jacob, Marie-Christine, Robillard, Nelly, Wuilleme, Soraya, Andre-Kerneis, Elisabeth, Cornet, Edouard, Salaun, Véronique, Bennami, Hind, Lhoumeau, Anne-Catherine, Arnoulet, Christine, JACQMIN, Hugues, NEYMAN, Nicolas, Latger-Cannard, Véronique, Massin, Fredéric, Lainey, Elodie, Le Garff-Tavernier, Magali, Costopoulos, Myrto, Roussel, Mickael, Mayeur-Rousse, Caroline, Eischen, Alice, Raggeneau, Victoria, Derrieux, Coralie, Maurer, Maxime, Asnafi, Vahid, Trinquand, Amélie, Brouzes, Chantal, and Lhermitte, Ludovic

Abstract

Flow cytometry is broadly used for the identification, characterization, and monitoring of hematological malignancies. However, the use of clinical flow cytometry is restricted by its lack of reproducibility across multiple centers. Since 2006, the EuroFlow consortium has been developing a standardized procedure detailing the whole process from instrument settings to data analysis. The FranceFlow group was created in 2010 with the intention to educate participating centers in France about the standardized instrument setting protocol (SOP) developed by the EuroFlow consortium and to organise several rounds of quality controls (QCs) in order to evaluate the feasibility of its application and its results. Here, we report the 5 year experience of the FranceFlow group and the results of the seven QCs of 23 instruments, involving up to 19 centers, in France and in Belgium. The FranceFlow group demonstrates that both the distribution and applicability of the SOP have been successful. Intercenter reproducibility was evaluated using both normal and pathological blood samples. Coefficients of variation (CVs) across the centers were <7% for the percentages of cell subsets and <30% for the median fluorescence intensities (MFIs) of the markers tested. Intracenter reproducibility provided similar results with CVs of <3% for the percentages of the majority of cell subsets, and CVs of <20% for the MFI values for the majority of markers. Altogether, the FranceFlow group show that the 19 participating labs might be considered as one unique laboratory with 23 identical flow cytometers able to reproduce identical results. Therefore, SOP significantly improves reproducibility of clinical flow in hematology and opens new avenues by providing a robust companion diagnostic tool for clinical trials in hematology. © 2019 International Society for Advancement of Cytometry.

Published
2019

10. Standardization of Flow Cytometric Immunophenotyping for Hematological Malignancies: The FranceFlow Group Experience

Author

Solly, Françoise, primary, Angelot‐Delettre, Fanny, additional, Ticchioni, Michel, additional, Geneviève, Franck, additional, Rambaud, Hubert, additional, Baseggio, Lucile, additional, Plesa, Adriana, additional, Debliquis, Agathe, additional, Garnache‐Ottou, Francine, additional, Roggy, Anne, additional, Campos, Lydia, additional, Aanei, Carmen, additional, Rosenthal‐Allieri, Alessandra, additional, Georget, Marie‐Thérèse, additional, Lachot, Sébastien, additional, Jacob, Marie‐Christine, additional, Robillard, Nelly, additional, Wuilleme, Soraya, additional, Andre‐Kerneis, Elisabeth, additional, Cornet, Edouard, additional, Salaun, Véronique, additional, Bennami, Hind, additional, Lhoumeau, Anne‐Catherine, additional, Arnoulet, Christine, additional, Jacqmin, Hugues, additional, Neyman, Nicolas, additional, Latger‐Cannard, Véronique, additional, Massin, Fredéric, additional, Lainey, Elodie, additional, Le Garff‐Tavernier, Magali, additional, Costopoulos, Myrto, additional, Roussel, Mickael, additional, Mayeur‐Rousse, Caroline, additional, Eischen, Alice, additional, Raggeneau, Victoria, additional, Derrieux, Coralie, additional, Maurer, Maxime, additional, Asnafi, Vahid, additional, Trinquand, Amélie, additional, Brouzes, Chantal, additional, and Lhermitte, Ludovic, additional

Published
2019
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11. Etiology and Outcomes of Thrombotic Microangiopathies

Author

Bayer, Guillaume, primary, von Tokarski, Florent, additional, Thoreau, Benjamin, additional, Bauvois, Adeline, additional, Barbet, Christelle, additional, Cloarec, Sylvie, additional, Mérieau, Elodie, additional, Lachot, Sébastien, additional, Garot, Denis, additional, Bernard, Louis, additional, Gyan, Emmanuel, additional, Perrotin, Franck, additional, Pouplard, Claire, additional, Maillot, François, additional, Gatault, Philippe, additional, Sautenet, Bénédicte, additional, Rusch, Emmanuel, additional, Buchler, Matthias, additional, Vigneau, Cécile, additional, Fakhouri, Fadi, additional, and Halimi, Jean-Michel, additional

Published
2019
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14. Multicentric Standardized Flow Cytometry Routine Assessment of Patients With Sepsis to Predict Clinical Worsening

Author

Daix, Thomas, primary, Guerin, Estelle, additional, Tavernier, Elsa, additional, Mercier, Emmanuelle, additional, Gissot, Valérie, additional, Hérault, Olivier, additional, Mira, Jean-Paul, additional, Dumas, Florence, additional, Chapuis, Nicolas, additional, Guitton, Christophe, additional, Béné, Marie C., additional, Quenot, Jean-Pierre, additional, Tissier, Cindy, additional, Guy, Julien, additional, Piton, Gaël, additional, Roggy, Anne, additional, Muller, Grégoire, additional, Legac, Éric, additional, de Prost, Nicolas, additional, Khellaf, Mehdi, additional, Wagner-Ballon, Orianne, additional, Coudroy, Rémi, additional, Dindinaud, Elodie, additional, Uhel, Fabrice, additional, Roussel, Mikaël, additional, Lafon, Thomas, additional, Jeannet, Robin, additional, Vargas, Frédéric, additional, Fleureau, Catherine, additional, Roux, Mickaël, additional, Allou, Kaoutar, additional, Vignon, Philippe, additional, Feuillard, Jean, additional, François, Bruno, additional, Di Vittorio, Adollës, additional, Lachot, Sébastien, additional, Georget, Marie-Thérèse, additional, Hanna, Joelle, additional, Bretonnière, Cédric, additional, Zambon, Olivier, additional, Nicolet, Laurent, additional, Brule, Noëlle, additional, Debord, Camille, additional, Le Bris, Yannick, additional, Robillard, Nelly, additional, Dargent, Auguste, additional, Bretagnol, Anne, additional, Mathonnet, Armelle, additional, Boulain, Thierry, additional, Barbier, François, additional, Benzekri, Dalila, additional, Runge, Isabelle, additional, Kamel, Toufik, additional, Cecchini, Jérôme, additional, Bouyer, Sabrina, additional, Robert, René, additional, and Ouattara, Alexandre, additional

Published
2018
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15. Reactive oxygen species levels differentiate CD34+human progenitors based on CD38expression

Author

Vignon, Christine, Lachot, Sébastien, Foucault, Amélie, Ravalet, Noémie, Gyan, Emmanuel, Picou, Frédéric, Herault, Beatrice, Le Nail, Louis‐Romée, Bene, Marie C., and Herault, Olivier

Abstract

Low reactive oxygen species (ROS) levels are well‐established characteristics of mouse hematopoietic stem cells (HSCs). However, little is known about these levels in human HSCs. This study aimed at quantifying ROS levels in human CD34+CD38lowand CD34+CD38highhuman progenitors from bone marrow, cord blood and cells mobilized for autologous HSC transplantation. A specifically devised multiparameter flow cytometry method was used to quantify ROS levels (H2DCFDA staining) in sub‐populations of primary cells. Results were confirmed by assessing gene expression level of the ROS scavenger GPX3,a key determinant of HSC self‐renewal, in sorted CD34+CD38lowand CD34+CD38highcells. CD34+CD38lowcells from bone marrow and cord blood displayed significantly lower levels of ROS than CD34+CD38highcells and other leukocytes. Moreover, the correlation between ROS and GPX3expression was verified in sorted CD34+CD38lowand CD34+CD38highcells. These results confirm, in human, data previously reported in mice. Moreover, the flow cytometry assay we developed could allow for a more precise enumeration of repopulating primitive progenitors in the course of HSC transplantation.

Published
2020
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