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4 results on '"Lachagari, Vijaya Bhaskar Reddy"'

2. Genome‐wide miRNAs profiles of pearl millet under contrasting high vapor pressure deficit reveal their functional roles in drought stress adaptations

Author

Palakolanu, Sudhakar Reddy, primary, Gupta, Saurabh, additional, Yeshvekar, Richa K., additional, Chakravartty, Navajeet, additional, Kaliamoorthy, Sivasakthi, additional, Shankhapal, Aishwarya Rajesh, additional, Vempati, Ashwini Soumya, additional, Kuriakose, Boney, additional, Lekkala, Sivarama Prasad, additional, Philip, Manuel, additional, Perumal, Rajadurai Chinnasamy, additional, Lachagari, Vijaya Bhaskar Reddy, additional, and Bhatnagar‐Mathur, Pooja, additional

Published
2021
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3. Genome-wide miRNAs profiles of pearl millet under contrasting high vapor pressure deficit reveal their functional roles in drought stress adaptations.

Author

Palakolanu, Sudhakar Reddy, Gupta, Saurabh, Yeshvekar, Richa K., Chakravartty, Navajeet, Kaliamoorthy, Sivasakthi, Shankhapal, Aishwarya Rajesh, Vempati, Ashwini Soumya, Kuriakose, Boney, Lekkala, Sivarama Prasad, Philip, Manuel, Perumal, Rajadurai Chinnasamy, Lachagari, Vijaya Bhaskar Reddy, and Bhatnagar-Mathur, Pooja

Subjects
PEARL millet, VAPOR pressure, MICRORNA, DROUGHT tolerance, DROUGHTS, ABIOTIC stress
Abstract

Pearl millet (Pennisetum glaucum [L.] R. Br.) is an important crop capable of growing in harsh and marginal environments, with the highest degree of tolerance to drought and heat stresses among cereals. Diverse germplasm of pearl millet shows a significant phenotypic variation in response to abiotic stresses, making it a unique model to study the mechanisms responsible for stress mitigation. The present study focuses on identifying the physiological response of two pearl millet high-resolution cross (HRC) genotypes, ICMR 1122 and ICMR 1152, in response to low and high vapor pressure deficit (VPD). Under high VPD conditions, ICMR 1152 exhibited a lower transpiration rate (Tr), higher transpiration efficiency, and lower root sap exudation than ICMR 1122. Further, Pg-miRNAs expressed in the contrasting genotypes under low and high VPD conditions were identified by deep sequencing analysis. A total of 116 known and 61 novel Pg-miRNAs were identified from ICMR 1152, while 26 known and six novel Pg-miRNAs were identified from ICMR 1122 genotypes, respectively. While Pg-miR165, 168, 170, and 319 families exhibited significant differential expression under low and high VPD conditions in both genotypes, ICMR 1152 showed abundant expression of Pg-miR167, Pg-miR172, Pg-miR396 Pg-miR399, Pg-miR862, Pg-miR868, Pg-miR950, Pg-miR5054, and Pg-miR7527 indicating their direct and indirect role in root physiology and abiotic stress responses. Drought responsive Pg-miRNA targets showed upregulation in response to high VPD stress, further narrowing down the miRNAs involved in regulation of drought tolerance in pearl millet. [ABSTRACT FROM AUTHOR]

Published
2022
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4. Deciphering the transcriptomic insight during organogenesis in Castor (Ricinus communisL.), Jatropha (Jatropha curcasL.) and Sunflower (Helianthus annuusL.)

Author

Puvvala, Sai Sudha, Muddanuru, Tarakeswari, Thangella, Padmavathi A. V., Kumar, O. Aniel, Chakravartty, Navajeet, Vettath, Vineeth Kodengil, Katta, A. V. S. K. Mohan, Lekkala, Sivarama Prasad, Kuriakose, Boney, Gupta, Saurabh, Mulpuri, Sujatha, and Lachagari, Vijaya Bhaskar Reddy

Abstract

Cultivation of the castor crop is hindered by various factors and one of the approaches for genetic improvement of the crop is through exploitation of biotechnological tools. Response of castor tissues to in vitro culture is poor which necessitated this study on understanding the molecular basis of organogenesis in cultured tissues of castor, through de novo transcriptome analysis and by comparing with jatropha and sunflower having good regeneration ability. Transcriptome profiling analysis was carried out with hypocotyl explants from castor, jatropha and cotyledons from sunflower cultured on MS media supplemented with different concentrations of hormones. Differentially expressed genes during dedifferentiation and organogenic differentiation stages of callus included components of auxin and cytokinin signaling, secondary metabolite synthesis, genes encoding transcription factors, receptor kinases and protein kinases. In castor, many genes involved in auxin biosynthesis and homeostasis like WAT1, vacuolar transporter genes, transcription factors like short root like protein were down-regulated while genes like DELLA were up-regulated accounting for regeneration recalcitrance. Validation of 62 DEGs through qRT-PCR showed a consensus of 77.4% of the genes expressed. Overall study provides set of genes involved in the process of organogenesis in three oilseed crops which forms a basis for understanding and improving the efficiency of plant regeneration and genetic transformation in castor.

Published
2019
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