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236 results on '"Lacava, J"'

1. The Eukaryotic RNA Exosome

Author

LaCava, J., Vaňáčová, Štěpánka, and Damage and Repair in Cancer Development and Cancer Treatment (DARE)

Abstract

This volume provides a cross-section of RNA exosome research protocols, applied to a diversity of model organisms. Chapters guide readers through methods that e.g. delineate eukaryotic exosomes’ origins in prokaryotes, probe its RNA substrates, adapter complexes, and macromolecular interaction of networks, and establish critical structure-function relationships. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Eukaryotic RNA Exosome: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Published
2020

4. Nuclear RNA turnover

Author

LaCava, J., Milligan, L., Torchet, C., Houseley, J., and Tollervey, D.

Published
2004

9. Dissection of purified LINE-1 reveals distinct nuclear and cytoplasmic intermediates

Author

Molloy, K.R., primary, Taylor, M.S., additional, Altukhov, I., additional, Mita, P., additional, Jiang, H., additional, Adney, E.M., additional, Wudzinska, A., additional, Ischenko, D., additional, Burns, K.H., additional, Fenyö, D., additional, Chait, B.T., additional, Alexeev, D., additional, Rout, M.P., additional, Boeke, J.D., additional, and LaCava, J., additional

Published
2017
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16. Low-cost computational tools for the analysis and design of printed antennas

Author

Moreano V., R. F., Heckler, M. V. T., Tinoco S., A. F., and Lacava, J. C. da S.

Subjects
cylindrical structures, antenas de microfita, redes lineares não-uniformes, microstrip antennas, Ferramentas computacionais, estruturas cilíndricas, non-uniform linear arrays, Antenna Group, Computational tools
Published
2006

22. The human cap-binding complex is functionally connected to the nuclear RNA exosome.

Author

Andersen, PR, Domanski, M, Kristiansen, MS, Storvall, H, Ntini, E, Verheggen, C, Schein, A, Bunkenborg, J, Poser, I, Hallais, M, Sandberg, R, Hyman, A, LaCava, J, Rout, MP, Andersen, JS, Bertrand, E, Jensen, TH, Andersen, PR, Domanski, M, Kristiansen, MS, Storvall, H, Ntini, E, Verheggen, C, Schein, A, Bunkenborg, J, Poser, I, Hallais, M, Sandberg, R, Hyman, A, LaCava, J, Rout, MP, Andersen, JS, Bertrand, E, and Jensen, TH

Abstract

Nuclear processing and quality control of eukaryotic RNA is mediated by the RNA exosome, which is regulated by accessory factors. However, the mechanism of exosome recruitment to its ribonucleoprotein (RNP) targets remains poorly understood. Here we report a physical link between the human exosome and the cap-binding complex (CBC). The CBC associates with the ARS2 protein to form CBC-ARS2 (CBCA) and then further connects, together with the ZC3H18 protein, to the nuclear exosome targeting (NEXT) complex, thus forming CBC-NEXT (CBCN). RNA immunoprecipitation using CBCN factors as well as the analysis of combinatorial depletion of CBCN and exosome components underscore the functional relevance of CBC-exosome bridging at the level of target RNA. Specifically, CBCA suppresses read-through products of several RNA families by promoting their transcriptional termination. We suggest that the RNP 5' cap links transcription termination to exosomal RNA degradation through CBCN.

Published
2013

23. Electromagnetic Scattering Analysis of Simple Targets Embedded in Planar Multilayer Structures: Remote Sensing Applications

Author

Sant'Anna, Sidnei Joao Siqueira, Lacava, J. C. da S., Fernandes, David, Sant'Anna, Sidnei Joao Siqueira, Lacava, J. C. da S., and Fernandes, David

Abstract

Conventional water quality monitormg is expensive and time consuming. This is particularly problematic if the water bodies to be examined are large. Conventional techniques also bring about a high probability of undersampling (Hadjirnitsis et al. 2006). Conversely, remote sensing is a powerful tool to assess aquatic systems and is particularly useful in remote areas such as the Amazon lakes (Alcantara et a!., 2008). Data collected using this technique can provide a synoptic overview of such large aquatic environments, which could otherwise not be observed at a glance (Dekker et al. 1995). However, remote sensing is not easily applied to aquatic environment monitoring mainly because the mixture of the optically active substances (OAS) in the water. Several approaches have been proposed to cope with this issue such as derivative analysis (Goodin et al. 1993), the continuum removal (Kruse et al. 1993), and spectral mixture analysis (Novo and Shimabukuro, 1994; Oyama et al, 2009). The two first approaches are more suitable for hyperspectral images, whereas the spectral mixture analysis can be used for both hyperspectral and multispectral images. The Spectral Mixture Model (SMM) has largely been used for spectral mixture analysis, uncoupling the reflectance of each image pixel (Tyler et al. 2006) into the proportion of each water component contributing to the signal. The result of a spectral mixture analysis is a set of fraction images representing the proportion of each water component per image pixel. This technique has been applied to TM/Landsat images to determine the concentration of suspended particles (Mertes et al. 1993), chlorophyll-a concentration (Novo and Shimabukuro, 1994); as well as to MODIS images, to determine the chlorophyll-a concentration in the Amazon floodplain (Novo et al. 2006), to characterize the composition., isbn: 9789533070056, Pages: 619-644

Published
2009

47. Retrospective analysis on patients with male breast cancer

Author

Alvarez, A. M., primary, Quiroga, M. N. Gandur, additional, Cinat, G., additional, Iturbe, J., additional, Nissi, T. Said, additional, Lacava, J., additional, Cuartero, V., additional, Vallejo, C., additional, Leone, B., additional, and Mickiewicz, E., additional

Published
2007
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