1. SERS characterization of aggregated and isolated bacteria deposited on silver-based substrates
- Author
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Lea Rosselle, Eric Larquet, Anne Chantal Gouget-Laemmel, Simion Astilean, Cristina-Cassiana Andrei, Anne Moraillon, Sabine Szunerits, Monica Potara, François Ozanam, Endre Jakab, Nadia Skandrani, Rabah Boukherroub, Julie Bouckaert, Laboratoire de physique de la matière condensée, CNRS, École polytechnique,IP Paris, Palaiseau, France, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), European Project: 690836,H2020,H2020-MSCA-RISE-2015,PANG(2016), Laboratoire de Physique de la Matière Condensée (LPMC), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université de Lille, CNRS, Laboratoire de physique de la matière condensée [LPMC], Babes-Bolyai University [Cluj-Napoca] [UBB], Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN], and Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520
- Subjects
Surface-enhanced Raman scattering (SERS) ,Silver nanostructures ,Escherichia coli ,Staphylococcus aureus ,Aggregated and single bacteria ,Principal component analysis (PCA) ,Silver ,Surface Properties ,02 engineering and technology ,Spectrum Analysis, Raman ,medicine.disease_cause ,01 natural sciences ,Biochemistry ,Pilus ,Analytical Chemistry ,Cell wall ,symbols.namesake ,[CHIM.ANAL]Chemical Sciences/Analytical chemistry ,medicine ,Humans ,Molecule ,Escherichia coli Infections ,biology ,Chemistry ,010401 analytical chemistry ,Substrate (chemistry) ,[CHIM.MATE]Chemical Sciences/Material chemistry ,Staphylococcal Infections ,021001 nanoscience & nanotechnology ,biology.organism_classification ,0104 chemical sciences ,Biophysics ,symbols ,0210 nano-technology ,Raman spectroscopy ,Bacteria ,Raman scattering - Abstract
International audience; Surface-enhanced Raman scattering (SERS), based on the enhancement of the Raman signal of molecules positioned within a few nanometres from a structured metal surface, is ideally suited to provide bacterial-specific molecular fingerprints which can be used for analytical purposes. However, for some complex structures such as bacteria, the generation of reproducible SERS spectra is still a challenging task. Among the various factors influencing the SERS variability (such as the nature of SERS-active substrate, Raman parameters and bacterial specificity), we demonstrate in this study that the environment of Gram-positive and Gram-negative bacteria deposited on ultra-thin silver films also impacts the origin of the SERS spectra. In the case of densely packed bacteria, the obtained SERS signatures were either characteristic of the secretion of adenosine triphosphate for Staphylococcus aureus (S. aureus) or the cell wall and the pili/flagella for Escherichia coli (E. coli), allowing for an easy discrimination between the various strains. In the case of isolated bacteria, SERS mapping together with principal component analysis revealed some variabilities of the spectra as a function of the bacteria environment and the bactericidal effect of the silver. However, the variability does not preclude the SERS signatures of various E. coli strains to be discriminated.
- Published
- 2021
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