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1. Protective Effect of Argan and Olive Oils against LPS-Induced Oxidative Stress and Inflammation in Mice Livers

Author

Kamouni, Soufiane, Kebbaj, Riad, Andreoletti, Pierre, El Ktaibi, Abderrahim, Rharrassi, Issam, Essamadi, Abdelkhalid, El Kebbaj, M’hammed Saïd, Mandard, Stéphane, Latruffe, Norbert, Vamecq, Joseph, Nasser, Boubker, Cherkaoui-Malki, Mustapha, Laboratoire de Biochimie et Neurosciences [Settat, Maroc], Faculté des Sciences et Techniques de Settat-Université Hassan I [Maroc], Laboratoire des Sciences et Technologies de la Santé [Settat, Maroc], Institut Supérieur des Sciences de la Santé [Settat, Maroc] ( ISSS ) -Université Hassan I [Settat, Maroc], Laboratoire Bio-PeroxIL. Biochimie du Peroxysome, Inflammation et Métabolisme Lipidique ( Bio-PeroxIL ), Université de Bourgogne ( UB ), Laboratoire d’Anatomie Pathologique [Marrakech, Maroc], Hôpital Militaire Avicenne [Marrakech, Maroc], Laboratoire de Biochimie [Casablanca, Maroc], Université Hassan II Ain Chock, Casablanca.-Faculté des Sciences Ain Chock Casablanca, Equipe LIPNESS (LNC - U1231) ( LIPNESS ), Lipides - Nutrition - Cancer [Dijon - U1231] ( LNC ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Maladies RAres du DEveloppement embryonnaire et du MEtabolisme : du Phénotype au Génotype et à la Fonction ( RADEME ), Hôpital Jeanne de Flandre [Lille]-Université de Lille-Centre Hospitalier Régional Universitaire de Lille ( CHRU de Lille ) -Clinique de Génétique médicale Guy Fontaine [CHRU LIlle]-Centre de référence maladies rares Anomalies du développement [CHRU Lille], Laboratoire d'Hormonologie, Métabolisme-Nutrition & Oncologie ( HMNO ), Centre Hospitalier Régional Universitaire [Lille] ( CHRU Lille ) -Département de Biochimie et Biologie Moléculaire-Centre de Biologie et Pathologie (CBP) Pierre-Marie Degand-INSERM Structures Partenaires, This work was supported by the Ministère de l’enseignement et de la Recherche, Morocco. The Projet PPR from the Centre National Pour la Recherche Scientifique et Technique (CNRST)-Morocco. The followship 'bourse Jeunes chercheurs' from Lafarge, Andzoa and Agrotech. The Action Intégrée of the Comité Mixte Inter-universitaire Franco-Marocain (CMIFM, AIMA/14/310, Campus France N° 30293PA) from the PHC Volubulis program, Ministère des Affaires Etrangères, the Conseil Régional de Bourgogne and the Ministère de l’enseignement et de la Recherche. The authors would like to acknowledge networking support by the COST Action CA 16112 NutRedOx (Personalized Nutrition in aging society: redox control of major age-related diseases), supported by COST (European Cooperation in Science and Technology)., Faculté des Sciences et Techniques [Settat] (FSTS), Université Hassan 1er [Settat]-Université Hassan 1er [Settat], Université Hassan 1er [Settat]-Institut Supérieur des Sciences de la Santé [Settat, Maroc] (ISSS), Laboratoire Bio-PeroxIL. Biochimie du Peroxysome, Inflammation et Métabolisme Lipidique (Bio-PeroxIL), Université de Bourgogne (UB), Faculté des Sciences Aïn Chock [Casablanca] (FSAC), Université Hassan II [Casablanca] (UH2MC)-Université Hassan II [Casablanca] (UH2MC), Equipe LIPNESS (LNC - U1231) (LIPNESS), Lipides - Nutrition - Cancer [Dijon - U1231] (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Maladies RAres du DEveloppement embryonnaire et du MEtabolisme : du Phénotype au Génotype et à la Fonction - ULR 7364 (RADEME), Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Laboratoire d'Hormonologie, Métabolisme-Nutrition & Oncologie (HMNO), Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Département de Biochimie et Biologie Moléculaire-Centre de Biologie et Pathologie (CBP) Pierre-Marie Degand-INSERM Structures Partenaires, INSERM Structures Partenaires-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre de Biologie et Pathologie (CBP) Pierre-Marie Degand-Département de Biochimie et Biologie Moléculaire, and Mandard, Stéphane

Subjects
argan oil, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Liver Diseases, Anti-Inflammatory Agents, lipopolysaccharides, Antioxidants, Article, cytokines, sepsis, lcsh:Chemistry, Mice, Liver, lcsh:Biology (General), lcsh:QD1-999, antioxidant enzymes, inflammation, [ SDV.MHEP ] Life Sciences [q-bio]/Human health and pathology, Dietary Supplements, Animals, Plant Oils, oxidative stress, Olive Oil, lcsh:QH301-705.5, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

International audience; Sepsis causes severe dysregulation of organ functions, via the development of oxidative stress and inflammation. These pathophysiological mechanisms are mimicked in mice injected with bacterial lipopolysaccharide (LPS). Here, protective properties of argan oil against LPS-induced oxidative stress and inflammation are explored in the murine model. Mice received standard chow, supplemented with argan oil (AO) or olive oil (OO) for 25 days, before septic shock was provoked with a single intraperitoneal injection of LPS, 16 hours prior to animal sacrifice. In addition to a rise in oxidative stress and inflammatory markers, injected LPS also caused hepatotoxicity, accompanied by hyperglycemia, hypercholesterolemia and hyperuremia. These LPS-associated toxic effects were blunted by AO pretreatment, as corroborated by normal plasma parameters and cell stress markers (glutathione: GSH) and antioxidant enzymology (catalase, CAT; superoxide dismutase, SOD and glutathione peroxidase, GPx). Hematoxylin–eosin staining revealed that AO can protect against acute liver injury, maintaining a normal status, which is pointed out by absent or reduced LPS-induced hepatic damage markers (i.e., alanine aminotransferase (ALT) and aspartate transaminase (AST)). Our work also indicated that AO displayed anti-inflammatory activity, due to down-regulations of genes encoding pro-inflammatory cytokines Interleukin-6 (IL-6) and Tumor Necrosis Factor-α (TNF-α) and in up-regulations of the expression of anti-inflammatory genes encoding Interleukin-4 (IL-4) and Interleukin-10 (IL-10). OO provided animals with similar, though less extensive, protective changes. Collectively our work adds compelling evidence to the protective mechanisms of AO against LPS-induced liver injury and hence therapeutic potentialities, in regard to the management of human sepsis. Activations of IL-4/Peroxisome Proliferator-Activated Receptors (IL-4/PPARs) signaling and, under LPS, an anti-inflammatory IL-10/Liver X Receptor (IL-10/LXR) route, obviously indicated the high potency and plasticity of the anti-inflammatory properties of argan oil.

Published
2017

2. Peroxisomal Acyl-CoA Oxidase Type 1: Anti-Inflammatory and Anti-Aging Properties with a Special Emphasis on Studies with LPS and Argan Oil as a Model Transposable to Aging

Author

Mustapha Cherkaoui-Malki, Gérard Lizard, Norbert Latruffe, Joseph Vamecq, Fatima-Ezzahra Saih, Riad El Kebbaj, M' Hammed Saïd El Kebbaj, Boubker Nasser, Pierre Andreoletti, Maladies RAres du DEveloppement embryonnaire et du MEtabolisme : du Phénotype au Génotype et à la Fonction - ULR 7364 (RADEME), Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Laboratoire Bio-PeroxIL. Biochimie du Peroxysome, Inflammation et Métabolisme Lipidique (Bio-PeroxIL), Université de Bourgogne (UB), Laboratoire de Biochimie et Neurosciences [Settat, Maroc], Faculté des Sciences et Techniques [Settat] (FSTS), Université Hassan 1er [Settat]-Université Hassan 1er [Settat], Université Hassan II [Casablanca] (UH2MC), and Université Hassan 1er [Settat]

Subjects
0301 basic medicine, Lipopolysaccharides, Aging, food.ingredient, [SDV]Life Sciences [q-bio], Anti-Inflammatory Agents, Argan oil, Inflammation, Review Article, Pharmacology, Biochemistry, 03 medical and health sciences, chemistry.chemical_compound, food, medicine, Acyl-CoA oxidase, Animals, Humans, Plant Oils, lcsh:QH573-671, Oxidase test, lcsh:Cytology, fungi, FGF19, Cell Biology, General Medicine, Peroxisome, Nordihydroguaiaretic acid, Disease Models, Animal, 030104 developmental biology, chemistry, ACOX1, Acyl-CoA Oxidase, medicine.symptom, Oxidoreductases
Abstract

To clarify appropriateness of current claims for health and wellness virtues of argan oil, studies were conducted in inflammatory states. LPS induces inflammation with reduction of PGC1-αsignaling and energy metabolism. Argan oil protected the liver against LPS toxicity and interestingly enough preservation of peroxisomal acyl-CoA oxidase type 1 (ACOX1) activity against depression by LPS. This model of LPS-driven toxicity circumvented by argan oil along with a key anti-inflammatory role attributed to ACOX1 has been here transposed to model aging. This view is consistent with known physiological role of ACOX1 in yielding precursors of specialized proresolving mediators (SPM) and with characteristics of aging and related disorders including reduced PGC1-αfunction and improvement by strategies rising ACOX1 (via hormonal gut FGF19 and nordihydroguaiaretic acid in metabolic syndrome and diabetes conditions) and SPM (neurodegenerative disorders, atherosclerosis, and stroke). Delay of aging to resolve inflammation results from altered production of SPM, SPM improving most aging disorders. The strategic metabolic place of ACOX1, upstream of SPM biosynthesis, along with ability of ACOX1 preservation/induction and SPM to improve aging-related disorders and known association of aging with drop in ACOX1 and SPM, all converge to conclude that ACOX1 represents a previously unsuspected and currently emerging antiaging protein.

Published
2017

3. Argan Oil-Mediated Attenuation of Organelle Dysfunction, Oxidative Stress and Cell Death Induced by 7-Ketocholesterol in Murine Oligodendrocytes 158N

Author

El Mostafa Karym, Meryam Debbabi, Michel Prost, Maryem Bezine, Randa Sghaier, Anne Vejux, Gérard Lizard, Stéphane Grégoire, Wiem Meddeb, Philippe Durand, Lucy Martine, Amira Zarrouk, Lionel Bretillon, Boubker Nasser, Thomas Nury, Asmaa Badreddine, Thibault Moreau, Mustapha Cherkaoui-Malki, Emmanuelle Prost-Camus, Laboratoire Bio-PeroxIL. Biochimie du peroxysome, inflammation et métabolisme lipidique [Dijon] (BIO-PEROXIL), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Université Hassan 1er [Settat], Université de Monastir - University of Monastir (UM), Faculté de médecine de Sousse [Ibn EL Jazzar], Institut préparatoire aux études scientifiques et techniques [La Marsa] (IPEST), Université de Sousse, Laboratoire des Venins et Biomolécules Thérapeutiques - Laboratory of Venoms and Therapeutic Biomolecules (LR11IPT08), Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), Laboratoire de Recherche Appliquée Spiral [Bourgogne] (LARA SPIRAL), Service de Neurologie générale, vasculaire et dégénérative (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), This work was supported by grants from: University of Bourgogne (Dijon, France), University of Monastir (Monastir, Tunisia), Aides et Services à Domicile (ASSAD) (Louhans, France) and the Association Bourguignonne pour les Aplications en Sciences de l’Information en Médecine (ABASIM, Dijon, France). This work was supported by the Action Intégrée of the Comité Mixte Inter-Universitaire Franco-Marocain (CMIFM, MA/14/310) from the Projet Hubert Curien (PHC) Volubilis/Toubkal program, the Ministère de l’Enseignement Supérieur and the Centre National Pour la Recherche Scientifique et Technique (CNRST, Morocco), the projet PPR from the (CNRST, Morocco), the Ministère des Affaires Etrangères, the Conseil Régional de Bourgogne, and the University of Bourgogne. We also thank Philip Bastable for English corrections (University Hospital, Dijon, France)., Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Laboratoire Bio-PeroxIL. Biochimie du Peroxysome, Inflammation et Métabolisme Lipidique ( Bio-PeroxIL ), Université de Bourgogne ( UB ), Laboratoire Bio-PeroxIL. Biochimie du peroxysome, inflammation et métabolisme lipidique [Dijon] ( BIO-PEROXIL ), Université de Bourgogne ( UB ) -Université Bourgogne Franche-Comté ( UBFC ), Nutrition, Aliments Fonctionnels et Santé Vasculaire, Université de Monastir ( UM ), Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Laboratoire de Recherche Appliquée Spiral [Bourgogne] ( LARA SPIRAL ), Modélisation mathématique et numérique ( M2N ), Conservatoire National des Arts et Métiers [CNAM] : EA7340, UMR 6578 : Adaptabilité Biologique et Culturelle ( UAABC ), Université de la Méditerranée - Aix-Marseille 2-Centre National de la Recherche Scientifique ( CNRS ), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Lipides - Nutrition - Cancer (U866) ( LNC ), Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ), Laboratoire de Biochimie et Neurosciences [Settat, Maroc], Faculté des Sciences et Techniques de Settat-Université Hassan I [Maroc], University of Bourgogne (Dijon, France) University of Monastir (Monastir, Tunisia) Aides et Services a Domicile (ASSAD) (Louhans, France) Association Bourguignonne pour les Aplications en Sciences de l'Information en Medecine (ABASIM, Dijon, France) Action Integree of the Comite Mixte Inter-Universitaire Franco-Marocain (CMIFM) from the Projet Hubert Curien (PHC) Volubilis/Toubkal program MA/14/310 Ministere de l'Enseignement Superieur Centre National Pour la Recherche Scientifique et Technique (CNRST, Morocco) projet PPR from the (CNRST, Morocco) Ministere des Affaires Etrangeres Conseil Regional de Bourgogne University of Bourgogne, and Lizard, Gérard

Subjects
MESH: Plant Oils/pharmacology, Antioxidant, argan oil, extra virgin olive oil, alpha-tocopherol, 7-ketocholesterol, mitochondria, lysosome, peroxisome, oxiapoptophagy, 158N murine, oligodendrocytes, assay interference compounds, polyunsaturated fatty-acids, smooth-muscle-cells, blood-brain-barrier, pear seed oil, alzheimers-disease, docosahexaenoic acid, mediterranean diet, multiple-sclerosis, medicine.medical_treatment, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, MESH: Antioxidants/pharmacology, Apoptosis, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, Antioxidants, MESH: Lipid Peroxidation, lcsh:Chemistry, Lipid peroxidation, Mice, MESH: Oligodendroglia/drug effects, MESH: Animals, MESH: Oxidative Stress/drug effects, lcsh:QH301-705.5, Spectroscopy, chemistry.chemical_classification, General Medicine, régime méditerranéen, α-tocopherol, 158N murine oligodendrocytes, 3. Good health, maladie d'alzheimer, mitochondrie, MESH: Lysosomes/drug effects, Article, Inorganic Chemistry, 03 medical and health sciences, food, huile d'olive vierge, acide gras polyinsaturé, Food and Nutrition, sclérose en plaques, [ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Molecular Biology, tocopherol, huile d'argan, MESH: Cell Line, Tyrosol, MESH: Ketocholesterols/toxicity, 030104 developmental biology, chemistry, MESH: Mitochondria/drug effects, barrière hematoencephalique, Lipid Peroxidation, 0301 basic medicine, Argan oil, medicine.disease_cause, chemistry.chemical_compound, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Ketocholesterols, 2. Zero hunger, MESH: Peroxisomes/drug effects, Computer Science Applications, Oligodendroglia, Neuroprotective Agents, Biochemistry, Docosahexaenoic acid, Alimentation et Nutrition, Polyunsaturated fatty acid, food.ingredient, MESH: Apoptosis/drug effects, Context (language use), Biology, Catalysis, Cell Line, Peroxisomes, medicine, Animals, Plant Oils, Physical and Theoretical Chemistry, MESH: alpha-Tocopherol/pharmacology, MESH: Mice, MESH: Neuroprotective Agents/pharmacology, Organic Chemistry, Oxidative Stress, lcsh:Biology (General), lcsh:QD1-999, Lysosomes, Oxidative stress
Abstract

PMCID: PMC5666899; International audience; Argan oil is widely used in Morocco in traditional medicine. Its ability to treat cardiovascular diseases is well-established. However, nothing is known about its effects on neurodegenerative diseases, which are often associated with increased oxidative stress leading to lipid peroxidation and the formation of 7-ketocholesterol (7KC) resulting from cholesterol auto-oxidation. As 7KC induces oxidative stress, inflammation and cell death, it is important to identify compounds able to impair its harmful effects. These compounds may be either natural or synthetic molecules or mixtures of molecules such as oils. In this context: (i) the lipid profiles of dietary argan oils from Berkane and Agadir (Morocco) in fatty acids, phytosterols, tocopherols and polyphenols were determined by different chromatographic techniques; and (ii) their anti-oxidant and cytoprotective effects in 158N murine oligodendrocytes cultured with 7KC (25-50 mu M; 24 h) without and with argan oil (0.1% v/v) or -tocopherol (400 mu M, positive control) were evaluated with complementary techniques of cellular and molecular biology. Among the unsaturated fatty acids present in argan oils, oleate (C18:1 n-9) and linoleate (C18:1 n-6) were the most abundant; the highest quantities of saturated fatty acids were palmitate (C16:0) and stearate (C18:0). Several phytosterols were found, mainly schottenol and spinasterol (specific to argan oil), cycloartenol, beta-amyrin and citrostadienol alpha- and gamma-tocopherols were also present. Tyrosol and protocatechic acid were the only polyphenols detected. Argan and extra virgin olive oils have many compounds in common, principally oleate and linoleate, and tocopherols. Kit Radicaux Libres (KRL) and ferric reducing antioxidant power (FRAP) tests showed that argan and extra virgin olive oils have anti-oxidant properties. Argan oils were able to attenuate the cytotoxic effects of 7KC on 158N cells: loss of cell adhesion, cell growth inhibition, increased plasma membrane permeability, mitochondrial, peroxisomal and lysosomal dysfunction, and the induction of oxiapoptophagy (OXIdation + APOPTOsis + autoPHAGY). Altogether, our data obtained in 158N oligodendrocytes provide evidence that argan oil is able to counteract the toxic effects of 7KC on nerve cells, thus suggesting that some of its compounds could prevent or mitigate neurodegenerative diseases to the extent that they are able to cross the blood-brain barrier.

Published
2017

4. Improvements of ram semen quality using cactus seed oil during liquid preservation in Tris egg yolk and skim milk based extenders

Author

Larbi Allai, Boubker Nasser, Bouchra El Amiri, Noureddine Louanjli, Jesus Contell, Xavier Druart, Centre Régional de la Recherche Agronomique de Settat, Institut National de la Recherche Agronomique du Maroc (INRA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Biologie Médicale et Biologie de la Reproduction, LABOMAC, Recherche/Development Department, PROISER SL, Laboratoire de Biochimie et Neurosciences [Settat, Maroc], Faculté des Sciences et Techniques [Settat] (FSTS), Université Hassan 1er [Settat]-Université Hassan 1er [Settat], Campus France through two PRAD projects (Programme de Recherche Agronomique pour le Développement), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Tris, endocrine system, food.ingredient, antioxidant activity, Semen, DNA fragmentation, Biology, solvent, ram, 03 medical and health sciences, Semen quality, chemistry.chemical_compound, 0302 clinical medicine, food, Food Animals, ovin, Yolk, Botany, Skimmed milk, sperm parameters, stockage au froid, rams, Food science, refrigerated storage, reactive oxygen species, 030219 obstetrics & reproductive medicine, bélier, urogenital system, diluant, 0402 animal and dairy science, food and beverages, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, 04 agricultural and veterinary sciences, 040201 dairy & animal science, Sperm, qualité de la semence, chemistry, Cactus, Animal Science and Zoology, cactus seed oil, motilité du sperme
Abstract

The purpose of this study was to evaluate the effect of addition of cactus seed oil (CSO) on a Tris egg yolk (TEY) and skim milk (SM) extenders, on the sperm quality parameters in ram semen chilled for 72 h. Semen collected from five adult fertile Boujaâd rams was pooled and diluted to a final concentration of 0.8 × 109 spermatozoa/ml in TEY and SM extenders supplemented with CSO at 0, 1, 2, 5, and 10% v/v. Semen was then stored at 5 °C and several sperm parameters (motility, viability, morphology, peroxidation and DNA fragmentation) were assessed after 8, 24, 48 and 72 h of storage. Results showed that the supplementation of TEY and SM extenders with 1% and 2% CSO, respectively contributed to enhance sperm motility and viability and decreased levels of peroxidation and DNA fragmentation during storage. Therefore, we conclude that the optimal concentration of CSO in ram semen extender is 1% and 2%. In addition, our results suggest that CSO in these concentrations can help to maintain ram semen quality during chilled storage.

Published
2017

5. Argan oil prevents down-regulation induced by endotoxin on liver fatty acid oxidation and gluconeogenesis and on peroxisome proliferator-activated receptor gamma coactivator-1 alpha, (PGC-1alpha), peroxisome proliferator-activated receptor gamma (PPARgamma) and estrogen related receptor alpha (ERRalpha)

Author

Gérard Lizard, Hammam I. El Hajj, Norbert Latruffe, Pierre Andreoletti, Mustapha Cherkaoui-Malki, Fatima-Ezzahra Saih, Joseph Vamecq, Riad El Kebbaj, Boubker Nasser, Youssef El Kharrassi, M'Hammed Saïd El Kebbaj, Stéphane Mandard, Laboratoire Bio-PeroxIL. Biochimie du Peroxysome, Inflammation et Métabolisme Lipidique (Bio-PeroxIL), Université de Bourgogne (UB), Laboratoire de Biochimie et Neurosciences [Settat, Maroc], Faculté des Sciences et Techniques [Settat] (FSTS), Université Hassan 1er [Settat]-Université Hassan 1er [Settat], Laboratoire des Sciences et Technologies de la Santé [Settat, Maroc], Université Hassan 1er [Settat]-Institut Supérieur des Sciences de la Santé [Settat, Maroc] (ISSS), Laboratoire d'Hormonologie, Métabolisme-Nutrition & Oncologie (HMNO), INSERM Structures Partenaires-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre de Biologie et Pathologie (CBP) Pierre-Marie Degand-Département de Biochimie et Biologie Moléculaire, Maladies RAres du DEveloppement embryonnaire et du MEtabolisme : du Phénotype au Génotype et à la Fonction - ULR 7364 (RADEME), Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Protéines de Transfert des lipides et métabolisme des lipoprotéines (U866, Lipides et nutrition, équipe 8), Lipides - Nutrition - Cancer (U866) (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Laboratoire de recherche sur les lipoprotéines et l’Athérosclérose [Casablanca, Maroc], Université Hassan II [Casablanca] (UH2MC)-Faculté des Sciences Ben M’si [[Casablanca, Maroc], This work was supported by the Action Intégrée of the Comité Mixte Inter-universitaire Franco-Marocain (CMIFM,AIMA/14/310,Campus France)from the PHCVol-ubilis/Toubkal program (No30293PA), Ministère des Affaires Etrangères, The Centre NationalPour la Recherche Scientifique et Technique(CNRST)-Morocco, the Conseil Régional de Bourgogne(PARI2012:A324, PARI2013:B135, CPS0009) and the Ministère de l’enseignement et de la Recherche (crédits récurrents)., Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Département de Biochimie et Biologie Moléculaire-Centre de Biologie et Pathologie (CBP) Pierre-Marie Degand-INSERM Structures Partenaires, Mandard, Stéphane, Laboratoire Bio-PeroxIL. Biochimie du Peroxysome, Inflammation et Métabolisme Lipidique ( Bio-PeroxIL ), Université de Bourgogne ( UB ), Faculté des Sciences et Techniques de Settat-Université Hassan I [Maroc], Institut Supérieur des Sciences de la Santé [Settat, Maroc] ( ISSS ) -Université Hassan I [Settat, Maroc], Laboratoire d'Hormonologie, Métabolisme-Nutrition & Oncologie ( HMNO ), Centre Hospitalier Régional Universitaire [Lille] ( CHRU Lille ) -Département de Biochimie et Biologie Moléculaire-Centre de Biologie et Pathologie (CBP) Pierre-Marie Degand-INSERM Structures Partenaires, Maladies RAres du DEveloppement embryonnaire et du MEtabolisme : du Phénotype au Génotype et à la Fonction ( RADEME ), Hôpital Jeanne de Flandre [Lille]-Université de Lille-Centre Hospitalier Régional Universitaire de Lille ( CHRU de Lille ) -Clinique de Génétique médicale Guy Fontaine [CHRU LIlle]-Centre de référence maladies rares Anomalies du développement [CHRU Lille], Lipides - Nutrition - Cancer (U866) ( LNC ), Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ) -Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ), and Université Hassan II Mohammedia - Casablanca - UH2MC (MOROCCO)-Faculté des Sciences Ben M’si [[Casablanca, Maroc]

Subjects
Peroxisome proliferator-activated receptor gamma, medicine.medical_specialty, OO, olive oil, Research paper, [SDV]Life Sciences [q-bio], Peroxisome proliferator-activated receptor, Biology, Biochemistry, Nuclear receptor 30, lcsh:Biochemistry, Estrogen-related receptor, Estrogen-related receptor alpha, Internal medicine, ACADS, acyl CoA dehydrogenase short-chain, ACADL, acyl CoA dehydrogenase long-chain, medicine, PGC-1α, peroxisome proliferator-activated receptor γ coactivator-1α, lcsh:QD415-436, Receptor, Beta oxidation, HNF-4α, hepatic nuclear factor-4α, chemistry.chemical_classification, ACADM, acyl CoA dehydrogenase medium-chain, PPARα, peroxisome proliferator-activated receptor α, ERRα, estrogen related receptor α, [ SDV ] Life Sciences [q-bio], PEPCK, phospoenolpyruvate carboxykinase, Gluconeogenesis, Beta-oxidation, Glut4, glucose transporter 4, [SDV] Life Sciences [q-bio], G6PH, glucose-6-phosphatase, Endocrinology, Glut2, glucose transporter 2, chemistry, Nuclear receptor, Argan oil, AO, Argan oil, ACOX1, acyl-CoA oxidase 1, Coactivator, LPS, lipopolysaccharide, Peroxisome proliferator-activated receptor alpha
Abstract

In patients with sepsis, liver metabolism and its capacity to provide other organs with energetic substrates are impaired. This and many other pathophysiological changes seen in human patients are reproduced in mice injected with purified endotoxin (lipopolysaccharide, LPS). In the present study, down-regulation of genes involved in hepatic fatty acid oxidation (FAOx) and gluconeogenesis in mice exposed to LPS was challenged by nutritional intervention with Argan oil. Mice given a standard chow supplemented or not with either 6% (w/w) Argan oil (AO) or 6% (w/w) olive oil (OO) prior to exposure to LPS were explored for liver gene expressions assessed by mRNA transcript levels and/or enzyme activities. AO (or OO) food supplementation reveals that, in LPS-treated mice, hepatic expression of genes involved in FAOx and gluconeogenesis was preserved. This preventive protection might be related to the recovery of the gene expressions of nuclear receptors peroxisome proliferator-activated receptor α (PPARα) and estrogen related receptor α (ERRα) and their coactivator peroxisome proliferator-activated receptor gamma coactivator-1α, (PGC-1α). These preventive mechanisms conveyed by AO against LPS-induced metabolic dysregulation might add new therapeutic potentialities in the management of human sepsis., Highlights • Argan oil prevents LPS-treated mice from liver dysregulation of FAOx and gluconeogenesis. • Argan oil improves hepatic expression of PPARα and ERRα, and their coactivators PGC-1α and Lipin-1. • New preventive mechanisms conveyed by Argan oil against LPS-induced metabolic dysregulation.

Published
2015

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