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239 results on '"Laboisse, C."'

4. Butyrate specifically modulates MUC gene expression in intestinal epithelial goblet cells deprived of glucose

Author

Gaudier, E., Jarry, A., Blottiere, H.M., de Coppet, P., Buisine, M.P., Aubert, J.P., Laboisse, C., Cherbut, C., and Hoebler, C.

Subjects
Gastric mucosa -- Research, Fatty acids -- Research, Biological sciences
Abstract

The mucus layer covering the gastrointestinal mucosa is considered the first line of defense against aggressions arising from the luminal content. It is mainly composed of high molecular weight glycoproteins called mucins. Butyrate, a short-chain fatty acid produced during carbohydrate fermentation, has been shown to increase mucin secretion. The aim of this study was to test 1) whether butyrate regulates the expression of various MUC genes, which are coding for protein backbones of mucins, and 2) whether this effect depends on butyrate status as the major energy source of colonocytes. Butyrate was provided at the apical side of human polarized colonic goblet cell line HT29-Cl.16E in glucose-rich or glucose-deprived medium. In glucose-rich medium, butyrate significantly increased MUC3 and MUC5B expression (1.6-fold basal level for both genes), tended to decrease MUC5AC expression, and had no effect on MUC2 expression. In glucose-deprived medium, i.e., when butyrate was the only energy source available, MUC3 and MUC5B increase persisted, whereas MUC5AC expression was significantly enhanced (3.7-fold basal level) and MUC2 expression was strikingly increased (23-fold basal level). Together, our findings show that butyrate is able to upregulate colonic mucins at the transcriptional level and even better when it is the major energy source of the cells. Thus the metabolism of butyrate in colonocytes is closely linked to some of its gene-regulating effects. The distinct effects of butyrate according to the different MUC genes could influence the composition and properties of the mucus gel and thus its protective function. mucin; short-chain fatty acids; energy source; human colonic cell line

Published
2004

5. Niflumic acid inhibits ATP-stimulated exocytosis in a mucin-secreting epithelial cell line

Author

Bertrand, C.A., Danahay, H., Poll, C.T., Laboisse, C., Hopfer, U., and Bridges, R.J.

Subjects
Chloride channels -- Research, Biological sciences
Abstract

ATP is an efficacious secretagogue for mucin and chloride in the epithelial cell line HT29-C1.16E. Mucin release has been measured as [[sup.3]H]glucosamine-labeled product in extracellular medium and as single-cell membrane capacitance increases indicative of exocytosis-related increases in membrane area. The calcium-activated chloride channel blocker niflumic acid, also reported to modulate secretion, was used to probe for divergence in the purinergic signaling of mucin exocytosis and channel activation. With the use of whole cell patch clamping, ATP stimulated a transient capacitance increase of 15 [+ or -] 4%. Inclusion of niflumic acid significantly reduced the ATP-stimulated capacitance change to 3 [+ or -] 1%, although normalized peak currents were not significantly different. Ratiometric imaging was used to assess intracellular calcium ([Ca.sup.2+.sub.i]) dynamics during stimulation. In the presence of niflumic acid, the ATP-stimulated peak change in [Ca.sup.2+.sub.i] was unaffected, but the initial response and overall time to [Ca.sup.2+.sub.i] peak were significantly affected. Excluding external calcium before ATP stimulation or including the capacitative calcium entry blocker La[Cl.sub.3] during stimulation muted the initial calcium transient similar to that observed with niflumic acid and significantly reduced peak capacitance change, suggesting that a substantial portion of the ATP-stimulated mucin exocytosis in HT29-C1.16E depends on a rapid, brief calcium influx through the plasma membrane. Niflumic acid interferes with this influx independent of a chloride channel blockade effect. capacitative calcium entry; membrane capacitance; chloride channels; fura 2

Published
2004

11. Purinergic agonists, but not cAMP, stimulate coupled granule fusion and Cl- conductance in HT29-Cl.16E

Author

Guo, X.W., Merlin, D., Laboisse, C., and Hopfer, U.

Subjects
Chloride channels -- Physiological aspects, Cell lines -- Research, Cell membranes -- Physiological aspects, Biological sciences
Abstract

Sub-clones of the human colonic cancer cell line HT-29 were used to measure the conductance and capacitance of Cl- across granule membranes. Differences in the Cl- conductance and membrane capacitance were observed in the two sub-clones with the addition of extracellular ATP. Similar observations were also seen with regards to the purinergic reactions in both conductance and capacitance after the addition of exocytosis inhibitor wortmannin. The association between purigenically activated Cl- and granule fusion indicated the location of Cl- in the granules.

Published
1997

20. Overexpression of the CD 155 gene in human colorectal carcinoma

Author

Masson, D, Jarry, A, Baury, B, Blanchardie, P, Laboisse, C, Lustenberger, P, and Denis, M G.

Subjects
Genetic disorders -- Analysis -- Genetic aspects, Tumor proteins -- Abnormalities -- Genetic aspects -- Analysis, Colorectal cancer -- Genetic aspects -- Analysis, Health, Analysis, Genetic aspects, Abnormalities
Abstract

Abstract Background and aims--The Tage4 gene (tumour associated glycoprotein E4) is overexpressed in rat colon tumours and Min mouse intestinal adenomas. The rat Tage4 protein has approximately 40% identity with [...]

Published
2001

22. G.P.156

Author

Mercier, S., primary, Küry, S., additional, Magot, A., additional, Bodak, N., additional, Bou-Hanna, C., additional, Cormier-Daire, V., additional, David, A., additional, Faivre, L., additional, Figarella-Branger, D., additional, Gherardi, R., additional, Goldenberg, A., additional, Hamel, A., additional, Igual, J., additional, Israël-Biet, D., additional, Kannengiesser, C., additional, Laboisse, C., additional, Caignec, C. Le, additional, Munnich, A., additional, Mussini, J.M., additional, Piard, J., additional, Puzenat, E., additional, Salort-Campana, E., additional, Soufir, N., additional, Thauvin, C., additional, Péréon, Y., additional, Mayosi, B., additional, Barbarot, S., additional, and Bézieau, S., additional

Published
2014
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25. [Histopathologic features of cytomegalovirus lymphadenitis in the 'immunocompetent' patient. Report of 7 cases]

Author

Joubert M, Morin C, Moreau A, Marie-Françoise Heymann, Laboisse C, and Gaillard F

Subjects
Adult, Male, Hyperplasia, Lymphadenitis, Cytomegalovirus Infections, Humans, Female, Middle Aged, Child, Immunohistochemistry, Aged
Abstract

The authors report seven cases of cytomegalovirus lymphadenitis in apparently immunocompetent patients. One patient presented with an infectious mononucleosis-like illness. The main presentation of the others was isolated cervical lymphadenopathies. The lymph node pathology showed aspecific lymphoid hyperplasia, resembling the human immunodeficiency virus related lymphadenopathy, associated with diagnostic inclusion cells. Infected cells were confined to areas of monocytoid B cell hyperplasia in all cases whereas exceptionally observed in germinal centers. Because of their variable appearance, serial sectioning was frequently necessary to disclose their characteristic features. In all cases, immunohistochemistry using an anti-cytomegalovirus antibody was positive and revealed more infected cells than detected by morphology alone. Except for the endothelial cell, the nature of infected cells remained undetermined. An alteration of the antigenic expression as a consequence of cell infection might be responsible for immunohistochemistry failure in the cell characterization.

Published
1996

26. [Post-transplantation of Hodgkin's disease. Clinico-pathologic study of 3 cases]

Author

Moreau A, Dantal J, Mf, Heymann, Gaillard F, Dubigeon P, Portier D, Moreau P, Noel Milpied, and Laboisse C

Subjects
Adult, Immunosuppression Therapy, Male, Herpesvirus 4, Human, Heart Transplantation, Humans, Transplantation, Homologous, Middle Aged, Hodgkin Disease, Immunohistochemistry, Kidney Transplantation
Abstract

As a frequent complication of immunosuppression, lymphoproliferative disorders affect approximately 2 % of allorgan transplant recipients. Most of them are EBV-associated-B-cell-lymphoproliferations. Other types of non Hodgkin's lymphomas and Hodgkin's disease, as observed in general population, have only rarely been reported in this group of patients. We report three cases of Hodgkin's disease, which were diagnosed in two renal transplant recipients and one heart transplant patient. They were associated with Epstein-Barr virus, as demonstrated by immunohistochemistry and in situ hybridization. EBV is frequently associated with Hodgkin's disease in the general population, and always implicated in AIDS-related Hodgkin's disease. However in transplant patients the rarity of Hodgkin's disease argues against a direct oncogenic role of this virus.

Published
1996

27. Patterns of expression of lineage-specific markers during the in vitro-induced differentiation of HT29 colon carcinoma cells

Author

Velcich A, Palumbo L, Anne JARRY, Laboisse C, Racevskis J, and Augenlicht L

Subjects
Mucin-3, Mucin-2, Carcinoma, Colforsin, Mucins, Cell Differentiation, Alkaline Phosphatase, Antigens, Differentiation, Carcinoembryonic Antigen, Neoplasm Proteins, Gene Expression Regulation, Neoplastic, Butyrates, Antigens, Neoplasm, Colonic Neoplasms, Tumor Cells, Cultured, Butyric Acid, Humans, Tetradecanoylphorbol Acetate, Intestinal Mucosa, Biomarkers
Abstract

The four different cell types present in the mature colon, absorptive enterocytes, mucus-secreting goblet cells, Paneth cells, and enteroendocrine cells, are believed to derive from a common precursor, the stem cell, anchored near the base of the crypt. Stem cell descendants undergo several rounds of cell division, creating a pool of transit cells that are committed to differentiation. The mechanisms by which committed cells are allocated to the different cell lineages of the intestine are poorly understood. We have used the colon carcinoma cell line HT29 and Cl.16E cells, a clonal derivative of HT29 cells, to investigate the regulation and pattern of expression of several markers (MUC2, MUC3, carcinoembryonic antigen, and alkaline phosphatase) that are associated with a more differentiated phenotype and that, in the mature cells, are lineage restricted. HT29 cells can express, upon exposure to the appropriate inducers, distinct intestinal specific markers; they are, therefore, considered multipotent, similar to the stem cells of the crypt. Conversely, Cl.16E cells are lineage restricted and respond to cell contact inhibition by expressing a fully differentiated goblet cell phenotype. We show that, in HT29 cells, different inducers (12-O-tetradecanoylphorbol-13-acetate, forskolin, and sodium butyrate) modulate specific sets of markers. Forskolin induces the expression of both MUC2 and MUC3, whereas 12-O-tetradecanoylphorbol-13-acetate is capable of inducing only MUC2, and sodium butyrate, only MUC3 gene expression. Carcinoembryonic antigen, a marker common to enterocytes and goblet cells; can be induced by all the agents, whereas the alkaline phosphatase gene, the expression of which is characteristic of enterocytes, is responsive solely to sodium butyrate treatment.(ABSTRACT TRUNCATED AT 250 WORDS)

Published
1995

41. Generation of Heme Oxygenase-1-Transgenic Rats

Author

Braudeau, C., primary, Bouchet, D., additional, Toquet, C., additional, Tesson, L., additional, Ménoret, S., additional, Iyer, S., additional, Laboisse, C., additional, Willis, D., additional, Jarry, A., additional, Buelow, R., additional, Anegon, I., additional, and Chauveau, C., additional

Published
2003
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42. G.P.156: Mutations in FAM111B cause Hereditary Fibrosing Poikiloderma with tendon contracture, myopathy and pulmonary fibrosis

Author

Mercier, S., Küry, S., Magot, A., Bodak, N., Bou-Hanna, C., Cormier-Daire, V., David, A., Faivre, L., Figarella-Branger, D., Gherardi, R., Goldenberg, A., Hamel, A., Igual, J., Israël-Biet, D., Kannengiesser, C., Laboisse, C., Caignec, C. Le, Munnich, A., Mussini, J.M., Piard, J., Puzenat, E., Salort-Campana, E., Soufir, N., Thauvin, C., Péréon, Y., Mayosi, B., Barbarot, S., and Bézieau, S.

Published
2014
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