9 results on '"Labode J"'
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2. Three-dimensional characteristics of the alveolar capillary network in infant and adult human lungs.
- Author
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Rößler G, Labode J, Schipke J, Tschanz SA, and Mühlfeld C
- Abstract
Background: A comprehensive understanding of vascular development in the human lung is still missing., Methods: Therefore, samples of infant (n = 5, 26 days to 18 months postnatally) and adult (n = 5, 20 to 40 years) human lungs were subjected to unbiased stereological estimation of the total number of capillary loops. Serial sections were segmented to visualize the alveolar capillary network (ACN) in 3D., Results: The number of capillary loops increased in parallel to lung volume from 26 days to 18 months, while in adults, it was not correlated to lung volume. In infant lungs, two capillary layers were separated by a connective tissue sheet with a growing number of interconnections. In adults, the mature ACN was almost, but not completely, single-layered. Here, the connective tissue was thinner but still centrally positioned, suggesting the persistence of interconnected parts of both layers of the previously double-layered ACN., Conclusions: Small parts of the capillaries remain double-layered and seem to be grouped around the thin connective tissue sheet, suggesting a different mechanism of microvascular maturation than simple fusion of the two layers. These spots are a potential basis for further alveolarization after completion of bulk formation., Impact: The 3D data offer a new conceptual approach to microvascular maturation of the lung. Microvascular maturation rather results from reduction than simple fusion of capillary fragments. Adult lungs maintain small double-layered capillary spots. These could offer a potential source of regeneration. The data are important to better understand normal and pathological lung development., (© 2024. The Author(s).) more...
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- 2024
- Full Text
- View/download PDF
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3. Targeted Stereology: Sampling heterogeneously distributed structures and lesions in the lung.
- Author
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Mühlfeld C, Schipke J, Labode J, and Ochs M
- Abstract
Stereology, the gold standard of lung morphometry, critically depends on sampling of tissue for analysis. Random sampling approaches guarantee each part of the organ an equal chance of being included in the analysis, hence they guarantee a representative sample of the whole. However, when biological or pathological structures of interest are rare and/or heterogeneously distributed over the whole lung, the random sampling approach can be inefficient or even result in meaningless data. In such cases, a targeted sampling approach can be useful which helps to relate the analytical items to an appropriate reference space. Targeted stereology greatly benefits from the increasing availability of multi-resolution imaging techniques at macroscopic and microscopic level as well as digital tools of segmentation. As such, the present article outlines two basic sampling scenarios: 1. In the first scenario, computed tomography and microscopy are subsequently used to segment the airway/arterial tree and perform stereological measurements on specific branches of the tree. 2. The second scenario deals with heterogeneous distribution of pathological lesions. This type of analysis can be divided into two stages: assessment of lesions of interest (LOI) within the lung and assessment of subcompartments within LOI. Taken together, targeted stereology has a thorough foundation in stereological theory and is not only able to significantly increase the efficiency of the analysis but also to yield new types of information that would be lost with the classical random sampling approach. more...
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- 2024
- Full Text
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4. Multiscale and multimodal imaging for three-dimensional vascular and histomorphological organ structure analysis of the pancreas.
- Author
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Salg GA, Steinle V, Labode J, Wagner W, Studier-Fischer A, Reiser J, Farjallah E, Guettlein M, Albers J, Hilgenfeld T, Giese NA, Stiller W, Nickel F, Loos M, Michalski CW, Kauczor HU, Hackert T, Dullin C, Mayer P, and Kenngott HG more...
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- Animals, Swine, X-Ray Microtomography methods, Islets of Langerhans diagnostic imaging, Islets of Langerhans blood supply, Tomography, X-Ray Computed methods, Imaging, Three-Dimensional methods, Pancreas diagnostic imaging, Pancreas blood supply, Multimodal Imaging methods
- Abstract
Exocrine and endocrine pancreas are interconnected anatomically and functionally, with vasculature facilitating bidirectional communication. Our understanding of this network remains limited, largely due to two-dimensional histology and missing combination with three-dimensional imaging. In this study, a multiscale 3D-imaging process was used to analyze a porcine pancreas. Clinical computed tomography, digital volume tomography, micro-computed tomography and Synchrotron-based propagation-based imaging were applied consecutively. Fields of view correlated inversely with attainable resolution from a whole organism level down to capillary structures with a voxel edge length of 2.0 µm. Segmented vascular networks from 3D-imaging data were correlated with tissue sections stained by immunohistochemistry and revealed highly vascularized regions to be intra-islet capillaries of islets of Langerhans. Generated 3D-datasets allowed for three-dimensional qualitative and quantitative organ and vessel structure analysis. Beyond this study, the method shows potential for application across a wide range of patho-morphology analyses and might possibly provide microstructural blueprints for biotissue engineering., (© 2024. The Author(s).) more...
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- 2024
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5. Location-specific pathology analysis of the monopodial pulmonary vasculature in a rabbit model of bronchopulmonary dysplasia-A pilot study.
- Author
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Labode J, Haberthür D, Hlushchuk R, Regin Y, Gie AG, Salaets T, Toelen J, and Mühlfeld C
- Subjects
- Animals, Humans, Infant, Newborn, Rabbits, Pilot Projects, Animals, Newborn, Lung pathology, Oxygen, Disease Models, Animal, Mammals, Bronchopulmonary Dysplasia, Hyperoxia pathology
- Abstract
The mammalian pulmonary vasculature consists of functionally and morphologically heterogeneous compartments. When comparing sets of lungs, for example, in disease models or therapeutic interventions, local changes may be masked by the overall heterogeneity of the organ structure. Therefore, alterations taking place only in a sub-compartment may not be detectable by global analysis. In the monopodial lung, the characterization of distinct vessel groups is difficult, due to the asymmetrical branching pattern. In this pilot study, a previously established method to classify segments of the monopodial pulmonary arterial tree into homogeneous groups was employed. To test its suitability for experimental settings, the method was applied to a hyperoxia (HYX, ≥95% oxygen) rabbit model of bronchopulmonary dysplasia and a normoxic control group (NOX, 21% oxygen). The method allowed the identification of morphological differences between the HYX and the NOX groups. Globally visible differences in lumen diameter were pinpointed to specific lung regions. Furthermore, local changes of wall dimension and cell layers in single compartments, that would not have been identifiable in an unfocused analysis of the whole dataset, were found. In conclusion, the described method achieves a higher precision in morphological studies of lung disease models, compared to a common, global analysis approach., (© 2023 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society.) more...
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- 2023
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6. Prematurity and Hyperoxia Have Different Effects on Alveolar and Microvascular Lung Development in the Rabbit.
- Author
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Rößler G, Labode J, Regin Y, Salaets T, Gie A, Toelen J, and Mühlfeld C
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- Infant, Newborn, Animals, Humans, Rabbits, Pregnancy, Female, Animals, Newborn, Cesarean Section, Lung, Pulmonary Alveoli, Disease Models, Animal, Premature Birth, Hyperoxia, Bronchopulmonary Dysplasia
- Abstract
Bronchopulmonary dysplasia (BPD) is a developmental disorder of infants born prematurely, characterized by disrupted alveolarization and microvascular maturation. However, the sequence of alveolar and vascular alterations is currently not fully understood. Therefore, we used a rabbit model to evaluate alveolar and vascular development under preterm birth and hyperoxia, respectively. Pups were born by cesarean section 3 days before term and exposed for 7 days to hyperoxia (95% O
2 ) or normoxia (21% O2 ). In addition, term-born rabbits were exposed to normoxia for 4 days. Rabbit lungs were fixed by vascular perfusion and prepared for stereological analysis. Normoxic preterm rabbits had a significantly lower number of alveoli than term rabbits. The number of septal capillaries was lower in preterm rabbits but less pronounced than the alveolar reduction. In hyperoxic preterm rabbits, the number of alveoli was similar to that in normoxic preterm animals; however, hyperoxia had a severe additional negative effect on the capillary number. In conclusion, preterm birth had a strong effect on alveolar development, and hyperoxia had a more pronounced effect on capillary development. The data provide a complex picture of the vascular hypothesis of BPD which rather seems to reflect the ambient oxygen concentration than the effect of premature birth. more...- Published
- 2023
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7. Analysis of the alveolar shape in 3-D.
- Author
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Reimelt AM, Vasilescu DM, Beare R, Labode J, Knudsen L, and Grothausmann R
- Subjects
- Humans, X-Ray Microtomography, Lung Volume Measurements, Lung diagnostic imaging, Pulmonary Alveoli diagnostic imaging
- Abstract
Mechanical forces affect the alveolar shape, depending on location and tissue composition, and vary during the respiratory cycle. This study performs alveolar morphomics in different lobes of human lungs using models generated from three-dimensional (3-D) micro-computed tomography (microCT) images. Cylindrical tissue samples (1.6 cm × 2 cm) were extracted from two nontransplantable donor lungs (one ex-smoker and one smoker, 3 samples per subject) that were air-inflated and frozen solid in liquid nitrogen vapor. Samples were scanned with microCT (11 µm/voxel). Within representative cubic regions of interest (5.5 mm edge length), alveoli were segmented to produce corresponding 3-D models from which quantitative data were obtained. The surface of segmented alveoli (n_alv_total = 23,587) was divided into individual planar surfaces (facets) and angles between facet normals were calculated. Moreover, the number of neighboring alveoli was estimated for every alveolus. In this study, we examined intraindividual differences in alveolar morphology, which were reproducible in the lungs of two subjects. The main aspects are higher mean alveolar volumes (v_alv: 6.64 × 10
6 and 6.63 × 106 µm3 vs. 5.78 × 106 and 6.29 × 106 µm3 ) and surface sizes (s_alv: 0.19 and 0.18 mm2 vs. 0.17 mm2 in both lower lobes) in both upper lung lobes compared with the lower lobes. An increasing number of facets (f_alv) from top to bottom (12 and 14 in the upper lobes; 14 and 15 in the lower lobes), as well as a decreasing number of alveolar neighbors (nei_alv: 9 and 8 in the upper lobes; 8 and 7 in the lower lobes) from the upper lobes to the lower lobes were observed. We could observe an increasing ratio of alveolar entrance size to the surface size of the alveoli from top to bottom (S_ratio_alv: 0.71 and 0.64 in the upper lobes, 0.73 and 0.70 in the lower lobes). The angles between facet normals (ang_alv) were larger in the upper lobes (67.72° and 62.44°) of both lungs than in the lower lobes (66.19° and 61.30°). By using this new approach of analyzing alveolar 3-D data, which enables the estimation of facet, neighbor, and shape characteristics, we aimed to establish the baseline measures for in-depth studies of mechanical conditions and morphology. more...- Published
- 2023
- Full Text
- View/download PDF
8. Evaluation of classifications of the monopodial bronchopulmonary vasculature using clustering methods.
- Author
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Labode J, Dullin C, Wagner WL, Myti D, Morty RE, and Mühlfeld C
- Subjects
- Mice, Animals, Humans, X-Ray Microtomography, Pulmonary Alveoli, Cluster Analysis, Mammals, Lung, Pulmonary Artery
- Abstract
Mammalian pulmonary arteries divide multiple times before reaching the vast capillary network of the alveoli. Morphological analyses of the arterial branches can be challenging because more proximal branches are likely biologically distinct from more peripheral parts. Thus, it is useful to group the arterial branches into groups of coherent biology. While the generational approach of dichotomous branching is straightforward, the grouping of arterial branches in the asymmetrically branching monopodial lung is less clear. Several established classification methods return highly dissimilar groupings when employed on the same organ. Here, we established a workflow allowing the quantification of grouping results for the monopodial lung and tested various methods to group the branches of the arterial tree into coherent groups. A mouse lung was imaged by synchrotron x-ray microcomputed tomography, and the arteries were digitally segmented. The arterial tree was divided into its individual segments, morphological properties were assessed from corresponding light microscopic scans, and different grouping methods were employed, such as (fractal) generation or (Strahler) order. The results were ranked by the morphological similarity within and dissimilarity between the resulting groups. Additionally, a method from the mathematical field of cluster analysis was employed for creating a reference classification. In conclusion, there were significant differences in method performance. The Strahler order was significantly superior to the generation system commonly used to classify human lung structure. Furthermore, a clustering approach indicated more precise ways to classify the monopodial lung vasculature exist., (© 2022. The Author(s).) more...
- Published
- 2022
- Full Text
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9. Combination of µCT and light microscopy for generation-specific stereological analysis of pulmonary arterial branches: a proof-of-concept study.
- Author
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Grothausmann R, Labode J, Hernandez-Cerdan P, Haberthür D, Hlushchuk R, Lobachev O, Brandenberger C, Gie AG, Salaets T, Toelen J, Wagner WL, and Mühlfeld C
- Subjects
- Animals, Female, Pregnancy, Rabbits, Imaging, Three-Dimensional, Pulmonary Artery ultrastructure, X-Ray Microtomography
- Abstract
Various lung diseases, including pulmonary hypertension, chronic obstructive pulmonary disease or bronchopulmonary dysplasia, are associated with structural and architectural alterations of the pulmonary vasculature. The light microscopic (LM) analysis of the blood vessels is limited by the fact that it is impossible to identify which generation of the arterial tree an arterial profile within a LM microscopic section belongs to. Therefore, we established a workflow that allows for the generation-specific quantitative (stereological) analysis of pulmonary blood vessels. A whole left rabbit lung was fixed by vascular perfusion, embedded in glycol methacrylate and imaged by micro-computed tomography (µCT). The lung was then exhaustively sectioned and 20 consecutive sections were collected every 100 µm to obtain a systematic uniform random sample of the whole lung. The digital processing involved segmentation of the arterial tree, generation analysis, registration of LM sections with the µCT data as well as registration of the segmentation and the LM images. The present study demonstrates that it is feasible to identify arterial profiles according to their generation based on a generation-specific color code. Stereological analysis for the first three arterial generations of the monopodial branching of the vasculature included volume fraction, total volume, lumen-to-wall ratio and wall thickness for each arterial generation. In conclusion, the correlative image analysis of µCT and LM-based datasets is an innovative method to assess the pulmonary vasculature quantitatively. more...
- Published
- 2021
- Full Text
- View/download PDF
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