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Background: This study aimed to examine the effect of a commercially available multi-ingredient powder (AG1 Ⓡ ) on the gut microbiome and assess the impact of AG1 Ⓡ on GI tolerability and other clinical safety markers in healthy men and women., Methods: Using a double-blind, randomized, two-arm, placebo-controlled, parallel design, we examined a 4-week daily supplementation regimen of AG1 Ⓡ vs. placebo (PL). Fifteen men and 15 women provided stool samples for microbiome analysis, questionnaires for digestive quality of life (DQLQ), and completed visual analog scales (VAS) and Bristol stool charts to assess stool consistency and bowel frequency before and after the 4-week intervention. Participant's blood work (CBC, CMP, and lipid panel) was also assessed before and after the 4-week intervention. Alpha diversity was determined by Shannon and Chao1 index scores and evaluated by a two-way ANOVA, beta diversity in taxonomic abundances and functional pathways was visualized using partial least squares-discriminant analyses and statistically evaluated by PERMANOVA. To identify key biomarkers, specific feature differences in taxonomic relative abundance and normalized functional pathway counts were analyzed by linear discriminant analysis (LDA) effect size (LEfSe). Questionnaires, clinical safety markers, and hemodynamics were evaluated by mixed factorial ANOVAs with repeated measures. This study was registered on clinicaltrials.gov (NCT06181214)., Results: AG1 Ⓡ supplementation enriched two probiotic taxa ( Lactobacillus acidophilus and Bifidobacterium bifidum ) that likely stem from the probiotics species that exist in the product, as well as L. lactis CH_LC01 and Acetatifactor sp900066565 ASM1486575v1 while reducing Clostridium sp000435835. Regarding community function, AG1 Ⓡ showed an enrichment of two functional pathways while diminishing none. Alternatively, the PL enriched six, but diminished five functional pathways. Neither treatment negatively impacted the digestive quality of life via DQLQ, bowel frequency via VAS, or stool consistency via VAS and Bristol. However, there may have been a greater improvement in the DQLQ score (+62.5%, p  = 0.058, d = 0.73) after four weeks of AG1 Ⓡ supplementation compared to a reduction (-50%) in PL. Furthermore, AG1 Ⓡ did not significantly alter clinical safety markers following supplementation providing evidence for its safety profile., Conclusions: AG1 Ⓡ can be consumed safely by healthy adults over four weeks with a potential beneficial impact in their digestive symptom quality of life.

The aim of this study was to assess the effects of guayusa extract and Nordic Lion's Mane (LM) on cognition. Using a randomized, double-blind, placebo-controlled, crossover design, we examined the effects of a single dose of 650 mg guayusa extract (AMT: AmaTea ® Max) vs. 1 g Nordic-grown Lion's Mane (LM) vs. placebo (PL). Participants attended three testing visits consisting of neuropsychological tests (Go/No-go, N-Back, and Serial 7 s tasks) assessing performance, subjective assessments of cognitive perception, and vital signs. Each assessment was measured at baseline (pre-ingestion) and 1 and 2 h post ingestion. AMT significantly ( p ≤ 0.05) improved the number of attempts during Serial 7s, total score, number of correct responses, total number of responses, and reaction time during N-Back and improved Go stimulus reaction time, but it reduced the percentage of correct responses in the No-go stimulus response during Go/No-go. LM significantly ( p ≤ 0.05) improved the number of attempts during Serial 7s and reaction time during N-Back and improved Go stimulus reaction time in Go/No-go. AMT improved mental clarity, focus, concentration, mood, and productivity at 1 and 2 h ( p < 0.05); the ability to tolerate stress at 1 h; and had greater ratings than LM and PL for mental clarity, focus, concentration, and productivity. PL improved focus and concentration at 1 h from baseline ( p ≤ 0.05). AMT and LM improved subjective ratings of "happiness compared to peers" and "getting the most out of everything" ( p < 0.05); however, this occurred earlier in LM (i.e., 1 h post ingestion). AMT uniquely elevated blood pressure from baseline. AMT significantly improved cognitive performance and self-perceived cognitive indices of affect over a 2 h period and perceptions of happiness 2 h post ingestion. In comparison, LM helped improve working memory, complex attention, and reaction time 2 h post ingestion and perceptions of happiness over a 2 h period.

This study assessed the acute effects of oral methylliberine (Dynamine TM ) supplementation on cognitive function and indices of well-being. This was a double-blind, randomized, within-subject crossover trial. In total, 25 healthy men and women (33.5 ± 10.7 yr, 172.7 ± 8.6 cm, 73.3 ± 11.0 kg) underwent pretesting before ingesting methylliberine (100 mg) or a placebo (PLA) for 3 days. On the fourth day, the participants were tested before their fourth dose (baseline) and every hour post-ingestion for 3 h. After a one-week washout period, the participants repeated testing with the alternate investigational product. The testing battery consisted of vitals, Stroop test, Trail Making Test-B, and visual analog scales that assessed various indices of well-being. Mixed factorial ANOVAs with repeated measures were used to assess all variables. There were significant ( p ≤ 0.050) interactions in terms of concentration, motivation, and mood. Methylliberine improved concentration at 1 and 3 h, motivation at 3 h, and mood at 1, 2, and 3 h ( p ≤ 0.050). Methylliberine improved energy, sustained energy, and mood in all participants to a greater extent than PLA at 1 h and 3 h relative to baseline ( p ≤ 0.050). PLA improved motivation at 1 and 2 h and mood at 2 h ( p ≤ 0.050). Methylliberine improved concentration, well-being, and the ability to tolerate stress to a greater extent than PLA at 3 h relative to baseline ( p ≤ 0.050). Women observed elevations in sustained energy at 1 and 3 h ( p ≤ 0.050) with methylliberine vs. PLA. Methylliberine had a negligible influence on cognitive function and vitals ( p > 0.050), and no adverse events were reported. Methylliberine significantly improved subjective feelings of energy, concentration, motivation, and mood, but not cognitive function. PLA improved motivation and mood at hours 1 and 2, while methylliberine sustained these benefits for longer. Methylliberine also improved concentration, well-being, and the ability to tolerate stress to a greater degree than PLA, while having no detrimental effects on vital signs. Methylliberine also seemed to have a positive impact on sustained energy in women.

A randomised, placebo-controlled, double-blind, parallel clinical study was performed to examine the effects of a probiotic- amylase (PRO) blend on gastrointestinal (GI) symptoms. Sixty men and women (44.4 ± 8.9 yr; 82.0 ± 18.4 kg; 170.3 ± 11.5 cm; 28.1 ± 4.6 kg/m2) were randomised into PRO (n = 29) or placebo (PLA: n = 31) groups. Participants exhibited mild to moderate GI symptoms and severity [via Gastrointestinal Symptom Rating Scale (GSRS)] to be eligible for participation. Participants were tested before (Baseline) and after (POST) 6 weeks of supplementation on various gastrointestinal indices, the GSRS (to assess GI symptoms, frequency, and severity), an anxiety questionnaire (GAD-7), and an overall well-being questionnaire (SF-36). Two (PRO vs PLA) × 2 (Baseline vs POST) mixed factorial ANOVAs were completed to assess group, time, and (group × time) interaction effects. Fifty-two subjects who completed the entire study were analysed (PRO: n = 25, PLA: n = 27). There were statistically significant (P≤0.05) interactions for bloating, GSRS score, and abdominal discomfort but time effects for flatulence, constipation, stool regularity, and GAD-7 total score. PRO significantly reduced GSRS score (∼60 vs 25%, d = 0.72), bloating (∼49% vs 25%, d = -0.63) and abdominal discomfort (59% vs 32%, d = -0.66) to a greater degree than PLA. PRO significantly reduced subjective feelings of irritability, pain, and overall health interference. Oral supplementation of the probiotic-amylase blend was very well tolerated. Our study showed that the probiotic-amylase blend reduced the GSRS score and other GI symptoms to a greater degree than PLA. Clinical trial registration: clinicaltrials.gov #NCT05614726.

Purpose: The primary purpose of this study was to examine the influence of different work-to-rest ratios on relative energy system utilization during short-term upper-body sprint interval training (SIT) protocols., Methods: Forty-two recreationally trained men were randomized into one of three training groups [10 s work bouts with 2 min of rest (10:2, n = 11) or 4 min of rest (10:4, n = 11), or 30 s work bouts with 4 min of rest (30:4, n = 10)] or a control group (CON, n = 10). Participants underwent six training sessions over 2 weeks with 4-6 'all-out' sprints. Participants completed an upper body Wingate test (30 s 'all-out' using 0.05 kg kg -1 of the participant's body mass) pre- and post-intervention from which oxygen consumption and blood lactate were used to estimate oxidative, glycolytic, and adenosine triphosphate-phosphocreatine (ATP-PCr) energy system provisions. An analysis of covariance was performed on all testing measurements collected at post with the associated pre-values used as covariates., Results: Relative energy contribution (p = 0.026) and energy expenditure (p = 0.019) of the ATP-PCr energy system were greater in 10:4 (49.9%; 62.1 kJ) compared to CON (43.1%; 47.2 kJ) post training. No significant differences were found between groups in glycolytic or oxidative energy contribution over a 30 s upper body Wingate test., Conclusion: SIT protocols with smaller work-to-rest ratios may enhance ATP-PCr utilization in a 30 s upper body Wingate over a 2-week intervention.

Background: Sex-based differences in metabolism and morphological characteristics may result in unique exercise responses during periods of limited oxygen availability. Purpose: To evaluate the effects of moderate normobaric hypoxia on the parameters of the work-time relationship during upper body exercise in women. Materials and Methods: Thirteen recreationally active women (age: 22.7 ± 2.6 years; height: 167 ± 8.6 cm; weight: 66.4 ± 9.7 kg; body fat: 27.6% ± 5% body fat) completed a maximal graded exercise test in both normobaric hypoxia (H; fraction of inspired oxygen (F i O 2 ) = 0.14) and normoxia (N; F i O 2  = 0.20) on an arm ergometer to determine peak oxygen uptake (VO 2 peak) and peak power output (PPO). Each participant completed four constant, work rate, arm-cranking time-to-exhaustion tests at 90%-120% PPO in both environmental conditions. Linear regression was used to estimate critical power (CP) and anaerobic capacity (W') through the work-time relationship during the constant work rate tests. Paired sample t -tests compared mean differences between VO 2 peak, PPO, CP, and W' between conditions (normoxia vs. hypoxia). Two-way (condition × intensity) repeated measures analysis of variance (ANOVA) was used to compare total work (TW) and time to exhaustion. Results: Hypoxia significantly reduced VO 2 peak (N: 1.73 ± 0.31 L·minute -1 vs. H: 1.62 ± 0.27 L·minute -1 , p  = 0.008), but had no effects on PPO (N: 78.08 ± 14.51 W vs. H: 75.38 ± 13.46 W, p  = 0.09), CP (N: 57.44 ± 18.89 W vs. H: 56.01 ± 12.36 W, p  = 0.55), and W' (N: 4.81 ± 1.01 kJ vs. H: 4.56 ± 0.91 kJ, p  = 0.51). No significant condition × intensity interactions were noted for TW or time to exhaustion ( p  > 0.05). Conclusions: Moderate normobaric hypoxia significantly reduced VO 2 peak, but had minimal effects on CP and W' using the work-time model in women.

The objective was to compare the metabolic influence of varying work-to-rest ratios during upper body sprint interval training (SIT). Forty-two recreationally-trained men were randomized into a training group [10 s work - 2 min of rest (10:2) or 4 min of rest (10:4), or 30 s work - 4 min of rest (30:4)] or a control group (CON). Participants underwent six training sessions over two weeks. Assessments consisted of a graded exercise test [maximal oxygen consumption (VO 2 peak) and peak power output (PPO)], four constant-work rate trials [critical power, anaerobic working capacity, and electromyographic fatigue threshold], and an upper body Wingate test (mean/peak power and total work). Post-training absolute and relative VO 2 peak was greater than pre-training for 30:4 (p = .005 and p = .009, respectively), but lower for CON (p = .001 and p = .006, respectively). Post-training PPO was greater in 30:4 (p < .001). No differences were observed during the constant-work rate trials or Wingate test. Traditional SIT appears to have enhanced VO 2 peak in the upper body over a short-term two-week intervention., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Objective: The present study evaluated the effects of repeated-sprint training in normobaric hypoxia and β-alanine supplementation (BA) on aerobic and anaerobic performance in recreationally active men., Methods: Participants were randomly assigned to one of the following groups: normoxia/β-alanine (NB, n = 11), normoxia/placebo (NP, n = 8), normobaric hypoxia/β-alanine (HB, n = 10) and normobaric hypoxia/placebo (HP, n = 9). All participants completed 8 training sessions over 4 weeks on a cycle ergometer either in normobaric hypoxia (oxygen fraction: FiO 2  = 14.2%) or normoxia (FiO 2  = 20.9%). Participants were instructed to consume a daily dosage of 6.4 g of BA or placebo. Changes in performance in a graded exercise test, repeated-sprint test (RST), and 3-minute all-out test (3MT) were examined before and after training and supplementation., Results: No between-group differences were observed for training volume or supplementation compliance. Anthropometric and hematological measures remained unchanged before and after intervention in all groups. A main effect of training condition was shown for oxygen consumption and power output at respiratory compensation point, average power output during the last sprint of the RST, heart rate recovery following the RST, and total work during the 3MT. These measures in the normobaric hypoxia groups were significantly (p < 0.05) higher than the normoxia groups, except for the heart rate recovery following the RST. A main effect of supplement was detected in anaerobic working capacity, with postintervention values in the BA groups being significantly (p < 0.05) higher than the placebo groups., Conclusions: Repeated-sprint training in hypoxia improved aerobic performance, exercise tolerance, cardiovascular recovery, and overall working capacity, while BA maintained the anaerobic working capacity. However, BA did not provide additional benefits with respect to attenuating fatigue or enhancing repeated-sprint performance.

Resistance training may differentially affect morphological adaptations along the length of uni-articular and bi-articular muscles. The purpose of this study was to compare changes in muscle morphology along the length of the rectus femoris (RF) and vastus lateralis (VL) in response to resistance training. Following a 2-wk preparatory phase, 15 resistance-trained men (24.0 ± 3.0 y, 90.0 ± 13.8 kg, 174.9 ± 20.7 cm) completed pre-training (PRE) assessments of muscle thickness (MT), pennation angle (PA), cross-sectional area (CSA), and echo-intensity in the RF and VL at 30, 50, and 70% of each muscle's length; fascicle length (FL) was estimated from respective measurements of MT and PA within each muscle and region. Participants then began a high intensity, low volume (4 x 3-5 repetitions, 3min rest) lower-body resistance training program, and repeated all PRE-assessments after 8 weeks (2 d ∙ wk-1) of training (POST). Although three-way (muscle [RF, VL] x region [30, 50, 70%] x time [PRE, POST]) repeated measures analysis of variance did not reveal significant interactions for any assessment of morphology, significant simple (muscle x time) effects were observed for CSA (p = 0.002) and FL (p = 0.016). Specifically, average CSA changes favored the VL (2.96 ± 0.69 cm2, p < 0.001) over the RF (0.59 ± 0.20 cm2, p = 0.011), while significant decreases in average FL were noted for the RF (-1.03 ± 0.30 cm, p = 0.004) but not the VL (-0.05 ± 0.36 cm, p = 0.901). No other significant differences were observed. The findings of this study demonstrate the occurrence of non-homogenous adaptations in RF and VL muscle size and architecture following 8 weeks of high-intensity resistance training in resistance-trained men. However, training does not appear to influence region-specific adaptations in either muscle., Competing Interests: The authors have declared that no competing interests exist.

Purpose: To evaluate the effects of moderate normobaric hypoxia on the parameters of the work-time relationship in the upper body., Methods: Twenty-one recreationally active men completed a graded exercise test in both normobaric hypoxia (H) and normoxia (N) to exhaustion on an arm ergometer to determine V̇O 2 peak and peak power output (PPO). Each participant completed four constant work-rate (CWR) arm-cranking tests at 90-120% PPO in both environmental conditions. Linear regression was used to estimate critical power (CP) and anaerobic working capacity (AWC) via work-time relationship during the CWR tests., Results: H significantly reduced V̇O 2 peak (N: 2.34 ± 0.34 L vs. H: 2.27 ± 0.34 L, p = 0.041), PPO (N: 125.29 ± 17.98 W vs. H: 121.11 ± 17.98 W, p = 0.001), and CP (N: 90.22 ± 12.88 W vs. H: 85.26 ± 9.64 W, p = 0.042), but had no effect on AWC (N: 9.16 ± 2.81 kJ vs. H: 8.75 ± 3.23 kJ, p = 0.600)., Discussion: Moderate normobaric hypoxia appears to reduce CP in the upper body but has a variable effect on AWC., (Copyright © 2017 Elsevier B.V. All rights reserved.)

Objective: β-alanine (BA) is a nonproteogenic amino acid that combines with histidine to form carnosine. The amount taken orally in individual doses, however, is limited due to symptoms of paresthesia that are associated with higher doses. The use of a sustained-release formulation has been reported to reduce the symptoms of paresthesia, suggesting that a greater daily dose may be possible. The purpose of the present study was to determine whether increasing the daily dose of BA can result in a similar increase in muscle carnosine in a reduced time., Methods: Eighteen men and twelve women were randomized into either a placebo (PLC), 6-g BA (6G), or 12-g BA (12G) groups. PLC and 6G were supplemented for 4 weeks, while 12G was supplemented for 2 weeks. A resting blood draw and muscle biopsy were obtained prior to (PRE) and following (POST) supplementation. Plasma and muscle metabolites were measured by high-performance liquid chromatography. The loss in peak torque (ΔPT) was calculated from maximal isometric contractions before and after 250 isokinetic kicks at 180°·sec -1 PRE and POST., Results: Both 12G (p = 0.026) and 6G (p = 0.004) increased muscle carnosine compared to PLC. Plasma histidine was decreased from PRE to POST in 12G compared to PLC (p = 0.002) and 6G (p = 0.001), but no group x time interaction (p = 0.662) was observed for muscle histidine. No differences were observed for any hematological measure (e.g., complete blood counts) or in symptoms of paresthesia among the groups. Although no interaction was noted in ΔPT, a trend (p = 0.073) was observed., Conclusion: Results of this investigation indicate that a BA supplementation protocol of 12 g/d -1 , using a sustained-release formulation, can accelerate the increase in carnosine content in skeletal muscle while attenuating paresthesia.

Heart rate deflection point (HRDP) can be determined through different mathematical-modeling procedures, such as bi-segmental linear regression (2SEG) or maximal distance model (Dmax). The purpose was to compare heart rate (HR) and oxygen consumption (VO 2 ) at HRDP when using 2SEG and Dmax, and to examine their relationships with respiratory compensation point (RCP) and running performance. Nineteen participants completed a graded exercise test (GXT), to determine HRDP and RCP, and a 5km treadmill time trial (5K time ). No differences were found in HR or VO 2 when comparing HRDP 2SEG , HRDP Dmax , and RCP. Strong correlations were found between HRDP 2SEG , HRDP Dmax , and RCP when using HR and VO 2 . No relationships were found between 5K time and HR at HRDP or RCP; however, strong relationships were found with VO2. While 2SEG and Dmax may be interchangeable in determining HRDP, VO 2 at HRDP and RCP yielded stronger relationships to 5K time than HR. Therefore, VO 2 at HRDP may be a better predictor of running performance than HR.

Purpose: The NF-κB signaling pathway regulates multiple cellular processes following exercise stress. This study aims to examine the effects of an acute lower-body resistance exercise protocol and subsequent recovery on intramuscular NF-κB signaling., Methods: Twenty-eight untrained males were assigned to either a control (CON; n = 11) or exercise group (EX; n = 17) and completed a lower-body resistance exercise protocol consisting of the back squat, leg press, and leg extension exercises. Skeletal muscle microbiopsies were obtained from the vastus lateralis pre-exercise (PRE), 1-hour (1H), 5-hours (5H), and 48-hours (48H) post-resistance exercise. Multiplex signaling assay kits (EMD Millipore, Billerica, MA, USA) were used to quantify the total protein (TNFR1, c-Myc) or phosphorylation status of proteins belonging to the NF-κB signaling pathway (IKKa/b, IkBα, NF-κB) using multiplex protein assay. Repeated measures ANOVA analysis was used to determine the effects of the exercise bout on intramuscular signaling at each time point. Additionally, change scores were analyzed by magnitude based inferences to determine a mechanistic interpretation., Results: Repeated measures ANOVA indicated a trend for a two-way interaction between the EX and CON Group (p = 0.064) for c-Myc post resistance exercise. Magnitude based inference analysis suggest a "Very Likely" increase in total c-Myc from PRE-5H and a "Likely" increase in IkBα phosphorylation from PRE-5H post-resistance exercise., Conclusion: Results indicated that c-Myc transcription factor is elevated following acute intense resistance exercise in untrained males. Future studies should examine the role that post-resistance exercise NF-κβ signaling plays in c-Myc induction, ribosome biogenesis and skeletal muscle regeneration.

Wang, R, Hoffman, JR, Tanigawa, S, Miramonti, AA, La Monica, MB, Beyer, KS, Church, DD, Fukuda, DH, and Stout, JR. Isometric mid-thigh pull correlates with strength, sprint, and agility performance in collegiate rugby union players. J Strength Cond Res 30(11): 3051-3056, 2016-The purpose of this investigation was to examine the relationships between isometric mid-thigh pull (IMTP) force and strength, sprint, and agility performance in collegiate rugby union players. Fifteen members of a champion-level university's club rugby union team (mean ± SD: 20.67 ± 1.23 years, 1.78 ± 0.06 m, and 86.51 ± 14.18 kg) participated in this investigation. One repetition maximum (1RM) squat, IMTP, speed (40 m sprint), and agility (proagility test and T-test) were performed during 3 separate testing sessions. Rate of force development (RFD) and force output at 30, 50, 90, 100, 150, 200, and 250 milliseconds of IMTP, as well as the peak value were determined. Pearson product-moment correlation analysis was used to examine the relationships between these measures. Performance in the 1RM squat was significantly correlated to the RFD between 90 and 250 milliseconds from the start of contraction (r's ranging from 0.595 to 0.748), and peak force (r = 0.866, p ≤ 0.05). One repetition maximum squat was also correlated to force outputs between 90 and 250 milliseconds (r's ranging from 0.757 to 0.816, p ≤ 0.05). Sprint time over the first 5 m in the 40 m sprint was significantly (p ≤ 0.05) correlated with peak RFD (r = -0.539) and RFD between 30 and 50 milliseconds (r's = -0.570 and -0.527, respectively). Time for the proagility test was correlated with peak RFD (r = -0.523, p ≤ 0.05) and RFD between 30 and 100 milliseconds (r's ranging from -0.518 to -0.528, p's < 0.05). Results of this investigation indicate that IMTP variables are significantly associated with strength, agility, and sprint performance. Future studies should examine IMTP as a potential tool to monitor athletic performance during the daily training of rugby union players.

The purpose of the present study was to evaluate the relationship between spatial awareness, agility, and distance covered in global positioning system (GPS) derived velocity zone classifications during a collegiate rugby match. Twelve American collegiate rugby union players (mean±SD; age: 21.2±1.4 y; weight: 85.0±16.0 kg; 7 forwards & 5 backs) on a single team volunteered to participate in this investigation. The distances travelled at low (walking/jogging; <2.7m/s), moderate (cruising/striding; 2.7-5.0 m/s), and high intensities (running/sprinting; >5.0 m/s) were measured for each player using GPS sensors and normalized according to playing time during an official USA Rugby match. Spatial awareness was measured as visual tracking speed from one core session of a 3-dimensional multiple-object-tracking speed (3DMOTS) test (1.35±0.59 cm·sec-1). Agility was assessed utilizing the pro agility (5.05±0.28 sec) and t drill (10.62±0.39 sec). Analysis of variance revealed that athletes travelled the greatest distance during walking/jogging (39.5±4.5 m·min-1) and least distance during running/sprinting (4.9±3.5 m·min-1). Pearson product moment correlations revealed that only distance covered while cruising/striding (20.9±6.5 m·min-1) was correlated to spatial awareness (r=0.798, p=0.002). Agility did not correlate to distance covered at any velocity zone or spatial awareness. Spatial awareness, as determined by 3DMOTS, appears to be related to the moderate intensity movement patterns of rugby union athletes.

Background: To test if the critical power (CP) concept can be utilized to determine the critical rest interval (CRI) using repeated sprint ability (RSA) testing with varying work-to-rest ratios., Methods: Twelve recreationally trained men (mean±SD; age 24.1±3.6 years) completed a graded exercise test and three RSA protocols with 6-second maximal sprints and varying rest intervals (12-36 s) on a cycle ergometer to determine CRI. Intermittent critical power (ICP) was calculated through the linear total work (TW) and time-to-exhaustion (TTE) relationship, whereas CRI was estimated using average work per sprint and ICP. Validation trials above and below CRI were conducted to evaluate physiological response. Repeated measures analysis of variance were used to analyze variables from RSA trials and changes in blood lactate, oxygen uptake (V̇O2), heart rate (HR), peak power, and TW throughout the validation trials., Results: Blood lactate, average V̇O2, V̇O2peak, and heart rate were significantly greater below CRI (8.94±4.89 mmol/L, 2.05±0.36 L/min, 2.84±0.48 L/min, and 151.14±18.46 bpm, respectively) when compared to above CRI (6.56±3.45 mmol/L, 1.78±0.26 L/min, 2.61±0.43 L/min, and 138.14±17.51 bpm). Significant interactions were found between above and below CRI for minimal V̇O2 response and maximal HR response, which were consistent with the heavy and severe exercise intensity domains., Conclusions: The use of the work-time relationship determined from RSA testing with varying rest intervals can be used to determine CRI and may distinguish between physiological responses related to exercise intensity domains.

La Monica, MB, Fukuda, DH, Miramonti, AA, Beyer, KS, Hoffman, MW, Boone, CH, Tanigawa, S, Wang, R, Church, DD, Stout, JR, and Hoffman, JR. Physical differences between forwards and backs in American collegiate rugby players. J Strength Cond Res 30(9): 2382-2391, 2016-This study examined the anthropometric and physical performance differences between forwards and backs in a championship-level American male collegiate rugby team. Twenty-five male rugby athletes (mean ± SD; age 20.2 ± 1.6 years) were assessed. Athletes were grouped according to position as forwards (n = 13) and backs (n = 12) and were evaluated on the basis of anthropometrics (height, weight, percent body fat [BF%]), cross-sectional area (CSA), muscle thickness (MT), and pennation angle (PA) of the vastus lateralis (VL), maximal strength (1 repetition maximum [1RM] bench press and squat), vertical jump power, midthigh pull (peak force [PF] and peak rate of force development [PRFD]), maximal aerobic capacity (V[Combining Dot Above]O2peak), agility (pro agility, T test), speed (40-m sprint), and a tethered sprint (peak velocity [PV], time to peak velocity, distance covered, and step rate and length). Comparisons between forwards and backs were analyzed using independent t-tests with Cohen's d effect size. Forwards were significantly different from backs for body weight (90.5 ± 12.4 vs. 73.7 ± 7.1 kg, p < 0.01; d = 1.60), BF% (12.6 ± 4.2 vs. 8.8 ± 2.1%, p ≤ 0.05; d = 1.10), VL CSA (38.3 ± 9.1 vs. 28.7 ± 4.7 cm, p < 0.01; d = 1.26), 1RM bench press (121.1 ± 30.3 vs. 89.5 ± 20.4 kg, p ≤ 0.05; d = 1.17), 1RM squat (164.6 ± 43.0 vs. 108.5 ± 31.5 kg, p < 0.01; d = 1.42), PF (2,244.6 ± 505.2 vs. 1,654.6 ± 338.8 N, p < 0.01; d = 1.32), PV (5.49 ± 0.25 vs. 5.14 ± 0.37 m·s, p ≤ 0.05; d = 1.04), and step length (1.2 ± 0.1 vs. 1.1 ± 0.1 m, p ≤ 0.05; d = 0.80). V[Combining Dot Above]O2peak was significantly (p ≤ 0.05, d = -1.20) higher in backs (54.9 ± 3.9 ml·kg·min) than in forwards (49.4 ± 4.4 ml·kg·min). No differences in agility performance were found between position groups. The results of this study provide descriptive information on anthropometric and performance measures on American male collegiate championship-level rugby players offering potential standards for coaches to use when developing or recruiting players.

This study compared the acute and chronic response of circulating plasma brain-derived neurotrophic factor (BDNF) to high-intensity low-volume (HI) and low-intensity high volume (HV) resistance training. Twenty experienced resistance-trained men (23.5 ± 2.6 y, 1.79 ± 0.05 m, 75.7 ± 13.8 kg) volunteered for this study. Before the resistance training program (PRE), participants performed an acute bout of exercise using either the HI [3-5 reps; 90% of one repetition maximum (1RM)] or HV (10-12 reps; 70% 1RM) training paradigm. The acute exercise protocol was repeated after 7 wk of training (POST). Blood samples were obtained at rest (BL), immediately (IP), 30 min (30P), and 60 min (60P) post exercise at PRE and POST. A three-way repeated measure ANOVA was used to analyze acute changes in BDNF concentrations during HI and HV resistance exercise and the effect of 7 wk of training. No training × time × group interaction in BDNF was noted (P = 0.994). Significant main effects for training (P = 0.050) and time (P < 0.001) in BDNF were observed. Significant elevations in BDNF concentrations were seen from BL at IP (P = 0.001), 30P (P < 0.001), and 60P (P < 0.001) in both HI and HV combined during PRE and POST. BDNF concentrations were also observed to increase from PRE to POST when collapsed across groups and time. No significant group × training interaction (P = 0.342), training (P = 0.105), or group (P = 0.238) effect were noted in the BDNF area under the curve response. Results indicate that BDNF concentrations are increased after an acute bout of resistance exercise, regardless of training paradigm, and are further increased during a 7-wk training program in experienced lifters., (Copyright © 2016 the American Physiological Society.)

This study investigated the effects of high-intensity interval training (HIIT) and β-hydroxy-β-methylbutyric free acid (HMB) supplementation on physical working capacity at the onset of neuromuscular fatigue threshold (PWC(FT)). Thirty-seven participants (22 men, 15 women; 22.8 ± 3.4 years) completed an incremental cycle ergometer test (graded exercise test [GXT]); electromyographic amplitude from the right vastus lateralis was recorded. Assessments occurred preceding (PRE) and after 4 weeks of supplementation (POST). Participants were randomly assigned to control (C, n = 9), placebo (P, n = 14), or supplementation (S, n = 14) groups. Both P and S completed 12 HIIT sessions, whereas C maintained normal diet and activity patterns. The PWC(FT) (W) was determined using the maximal perpendicular distance (D(MAX)) method. Electromyographic amplitude (μVrms) over time was used to generate a cubic regression. Onset of fatigue (TF) was the x-value of the point on the regression that was at D(MAX) from a line between the first and last data points. The PWC(FT) was estimated using TF and GXT power-output increments. The 2-way analysis of variance (ANOVA) (group × time) resulted in a significant interaction for PWC(FT) (F = 6.69, p = 0.004). Post hoc analysis with 1-way ANOVA resulted in no difference in PWC(FT) among groups at PRE (F = 0.87, p = 0.43); however, a difference in PWC(FT) was shown for POST (F = 5.46, p = 0.009). Post hoc analysis among POST values revealed significant differences between S and both P (p = 0.034) and C (p = 0.003). No differences (p = 0.226) were noted between P and C. Paired samples t-tests detected significant changes after HIIT for S (p < 0.001) and P (p = 0.016), but no change in C (p = 0.473). High-intensity interval training increased PWC(FT), but HMB with HIIT was more effective than HIIT alone. Furthermore, it seems that adding HMB supplementation with HIIT in untrained men and women may further improve endurance performance measures.

This study examined the influence of recovery time on fatigue indices, performance (total work [TW], peak power [PP], and mean power [MP]), and oxygen consumption during repeated sprint ability (RSA) on a cycle ergometer. Eight recreationally-trained men performed 3 RSA protocols consisting of 10 × 6 s sprints with 12 s, 18 s, and 24 s rest intervals between each sprint. Fatigue indices were determined as percent decrement (%Dec) and rate of decline using either a log transform method or standard slope approach for TW, PP, and MP during respective RSA protocols. The maximal VO2 value in response to given sprint intervals and the minimal VO2 value in response to given rest periods (VO2 work and VO2 rest, respectively) were recorded. A repeated measures analysis of variance was used to analyze all variables. Average VO2 work was not different among rest interval trials. Average VO2 rest with 12 s rest was greater than 18 s and 24 s (2.16 ± 0.17 L · min(-1), 1.91 ± 0.18 L · min(-1), 1.72 ± 0.15 L · min(-1), respectively), while 18 s was greater than 24 s. Average TW and MP were greater with 24 s rest than 12 s (4,604.44 ± 915.98 J vs. 4,305.46 ± 727.17 J, respectively), with no differences between RSA protocols for PP. No differences in %Dec were observed. Both methods of calculating rates of decline per sprint for PP and TW were greater during 12 s than 18 s or 24 s. Since changes were only noted between the 12 s and 24 s protocols, a 6 s differential in rest intervals may not be enough to elicit alterations in TW, PP, MP, or %Dec in RSA performance. Rate of decline may be a more sensitive measure of fatigue than %Dec.

The purpose of this study was to examine the effect of the L-Alanyl-L-Glutamine dipeptide (AG) on cognitive function and reaction time (RT) following endurance exercise. Twelve male endurance athletes (23.5 ± 3.7 y; 175.5 ± 5.4 cm; 70.7 ± 7.6 kg) performed four trials, each consisting of running on a treadmill at 70% of VO2max for 1h, then at 90% of VO2max until exhaustion. One trial consisted of no hydration (DHY), another required ingestion of only a sports electrolyte drink (ED) and two trials required ingestion of a low dose (LD; 300 mg·500 ml(-1)) and high dose (HD) of AG (1 g·500ml(-1)) added to the ED. Cognitive function and reaction tests were administered pre- and post-exercise. Magnitude based inferences were used to analyze ∆ cognitive function and ∆ reaction test data. Results indicated that DHY had a possible negative effect on number of hits in a 60-sec reaction test compared to LD and HD, while ED appeared to have a negative effect compared to HD. Analysis of lower body quickness indicated that LD and HD were likely improved in comparison to DHY. Performance on the serial subtraction test appeared to be possibly better in ED than DHY, while other comparisons between groups regarding cognitive function were unclear. In conclusion, rehydrating with AG during submaximal exercise may maintain or enhance subsequent RT in upper and lower body activities compared to DHY. These same effects were not apparent when participants consumed ED.