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This study evaluated the use of a xenogeneic acellular dermal matrix (XADM) combined with a modified coronally advanced flap (MCAF) and partial resin composite filling (anatomic crown plus 1 mm of the root surface) to treat multiple Type 1 gingival recessions associated with B+ noncarious cervical lesions (NCCLs). The study included 24 recessions in 10 patients. Bleeding on probing, probing depth, combined defect height, relative gingival recession, clinical attachment level, esthetics, and dentin hypersensitivity were recorded. After 6 months, the treatment provided statistically significant outcomes: It reduced the combined defect height by 2.02 ± 0.65 mm, increased clinical attachment level by 1.96 ± 0.83 mm, and increased keratinized tissue thickness by 0.46 ± 0.47 mm, and shallow probing depths were seen. Significant dentin hypersensitivity reduction and esthetic improvements were observed. Therefore, XADM associated with MCAF and partial restoration might be a promising alternative to autogenous grafts used to treat multiple gingival recessions associated with B+ NCCLs.

There are few studies comparing xenogeneic acellular dermal matrixes (XDMs) with connective tissue graft (CTG) to treat single gingival recession (GR). The present study involved a reanalysis of previous studies and comparison of CTG or XDM to treat single RT1/GR.Fifty patients from three previous randomized clinical trials treated either by CTG (n = 25) or XDM (n = 25) were evaluated after 6-month. Clinical, patient-centered, and esthetic parameters were assessed. Pearson's correlation and regression analyses were also performed.Greater recession reduction (RecRed), percentage of root coverage (%RC), and complete root coverage (CRC) were observed in the CTG group (p ≤ 0.02). Gingival thickness (GT) and keratinized tissue width (KTW) gains were higher in the CTG group. The CTG group showed better esthetic at 6-month. Logistic analyses reported that GT([OR] = 1.647CTG was superior for treating RT1/GR, providing better root coverage outcomes and tissue gains over time. GT, PH, and graft type were classified as predictors of CRC.CTG presented better outcomes to treat single RT1 gingival recession when compared to the XDM.

Made available in DSpace on 2022-05-01T10:19:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Background: Xenogeneic matrices (XMs) have been increasingly used for root coverage procedures. This study compared the use of two types of XM (collagen matrix [CM] and xenogeneic acellular dermal matrix [XDM]) associated with the coronally advanced flap technique (CAF) to treat single gingival recessions. Methods: Seventy-five patients presenting single RT1 gingival recession were treated by CAF (control group, n = 25), CAF+CM (test group 1, n = 25), or CAF+XDM (test group 2, n = 25) and completed 6-month follow-up. Clinical, patient-centered, and esthetic assessments were performed and intra- and intergroup differences were analyzed. Results: At 6 months, the mean recession reduction for CAF, CAF+CM, and CAF+XDM was 2.4 ± 0.8 mm, 2.4 ± 0.9 mm and 2.1 ± 0.8 mm, respectively (P > 0.05). The corresponding mean percentage of root coverage was 78.9% ± 26.2% for CAF, 78.0% ± 28.5% for CAF+CM, and 65.6% ± 26.9% for CAF+XDM (P > 0.05). Dentin hypersensitivity and esthetic conditions showed significantly improvements in all groups. Test groups presented significant gains in gingival thickness (GT; CAF+CM: 0.4 ± 0.3 mm; CAF+XDM: 0.4 ± 0.2 mm) compared to the control group (CAF: 0.0 ± 0.1 mm; P

Made available in DSpace on 2021-06-25T22:23:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-12-16 Introduction: Gingival recession (GR) is a prevalent problem that is related to esthetic demands and dentin hypersensitivity. Frequently, GR is associated with non-carious cervical lesion (NCCLs) forming a combined defect (CD), which requires restorative and surgical treatment. Connective tissue graft procedures allow predictable root coverage but can result in patient discomfort. Case Presentation: This first case report describes multiple GRs associated with B+ NCCL at teeth #4 and #6 of a 27-year-old patient with a thin periodontal phenotype. The aim of this report was to evaluate the 6-month outcome of a new volume-stable collagen matrix (VCMX) combined with modified coronally advanced flap (MCAF) and partial restoration to treat CDs. After 6 months, significant improvement was observed regarding CD coverage (69.05%), recession reduction (2.25 mm), gingival thickness gain (0.74 mm), and keratinized tissue width gain (0.75 mm). In addition, excellent esthetic evaluation and patient comfort were achieved by using the biomaterial. Conclusion: VCMX associated with MCAF and partial restoration may be an option to treat multiple recession defects plus B+ NCCLs. Sao Paulo State Univ UNESP, Inst Sci & Technol, Div Periodont, Ave Eng Francisco Jose Longo 777, Sao Jose Dos Campos, SP, Brazil Sao Paulo State Univ UNESP, Inst Sci & Technol, Div Periodont, Ave Eng Francisco Jose Longo 777, Sao Jose Dos Campos, SP, Brazil

Made available in DSpace on 2020-12-12T02:48:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 Background: This study was conducted to assess the clinical, immunological, and patient-centered outcomes of microcurrent electrotherapy on palatal wound healing. Methods: This was a parallel, double-masked randomized clinical trial, in which 53 patients with ridge preservation indications were selected and randomly assigned to one of two groups. In the control (sham) group (n = 27), palatal wounds, after free gingival grafts (FGG) harvest, received sham application of electrotherapy. In the test (electrotherapy treatment [EE]) group (n = 26), palatal wounds, after FGG harvest, received application of microcurrent electrotherapy protocol. Clinical parameters, patient-centered outcomes, and inflammatory markers were evaluated, up to 90 days postoperatively. Results: The EE group achieved earlier wound closure (P

Made available in DSpace on 2022-05-01T09:30:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Background: This study was conducted to evaluate the clinical, immunologic, and patient-centered outcomes of enamel matrix protein derivative (EMD) on excisional wounds in palatal mucosa. Materials: Forty-four patients in need of ridge preservation were randomly allocated into two groups: control group (n = 22): open palatal wound after free gingival graft (FGG) harvest and EMD group (n = 22): open palatal wound after FGG harvest that received 0.3 ml of EMD. Clinical and patient-centered parameters were analyzed for 3 months post-treatment. Wound fluid levels of inflammatory markers were assessed 3 and 7 days postoperatively. Results: No significant inter-group difference was observed in remaining wound area and re-epithelialization. EMD and control groups achieved wound closure and re-epithelialization 30 days postoperatively (p .05). EMD appeared to selectively modulate wound fluid levels of monocyte chemoattractant protein-1, macrophage inflammatory protein-1α, matrix metallopeptidase 9, and tissue inhibitor of metalloproteinases-2. Conclusion: Within the limits of the present study, it can be concluded that EMD application to excisional palatal wounds using the investigated protocol does not provide clinical healing benefits, despite an apparent modulation of selected inflammatory markers. São Paulo State University (UNESP) Division of Periodontics Institute of Science and Technology Division of Operative Dentistry Department of General Dentistry University of Maryland School of Dentistry Department of Prosthodontics and Periodontics Division of Periodontics Piracicaba Dental School College of Dentistry Division of Periodontology The Ohio State University (OSU) College of Dentistry - Lexington University of Kentucky São Paulo State University (UNESP) Division of Periodontics Institute of Science and Technology

Made available in DSpace on 2021-06-25T23:35:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-12 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Introduction: The use of platelet-rich fibrin (PRF) membranes has been evaluated for different purposes. However, few studies aimed to assess the effect of PRF membranes on peri-implant mucosal thickness augmentation. Thus, the aim was to evaluate whether the use of PRF membranes placed using the same surgical procedure of single implant installation in the anterior area of the maxilla can result in peri-implant mucosal thickness increase. Case series: Eight patients received single maxillary implants (anterior area), covered by three PRF membranes (2,500 rpm/12 minutes, RCFmax = 719.71 g). Clinical parameters (baseline and 3 months postoperative) and patient-centered parameters were evaluated. The buccal peri-implant mucosal thickness (BMT) and ridge defect (RD) parameters showed a statistically significant difference, with a gain of 0.7 +/- 0.3 mm in BMT and reduction of 0.8 +/- 0.3 mm in RD. Supracrestal tissue height (STH) was >3 mm after 3 months. Postoperative pain and number of analgesics showed low values (0.95 +/- 1.06 and 1.92 +/- 2.75), respectively, using a visual analog scale (VAS). Conclusion: The use of three PRF membranes along with single implant placement in the anterior maxilla led to an increase in the BMT. Sao Paulo State Univ Unesp, Div Periodont, Inst Sci & Technol, Ave Eng Francisco Jose Longo,777, BR-12245000 Sao Jose Dos Campos, SP, Brazil Sao Paulo State Univ Unesp, Div Integrated Clin, Inst Sci & Technol, Sao Paulo, Brazil Sao Paulo State Univ Unesp, Div Periodont, Inst Sci & Technol, Ave Eng Francisco Jose Longo,777, BR-12245000 Sao Jose Dos Campos, SP, Brazil Sao Paulo State Univ Unesp, Div Integrated Clin, Inst Sci & Technol, Sao Paulo, Brazil CNPq: 304269/2019-0

Stryphnodendron adstringens (Mart.) Coville is a medicinal plant known for its anti-inflammatory and antimicrobial properties. This study evaluated some biological activities of extract from S. adstringens. Antimicrobial activity was checked in planktonic cultures and monomicrobial biofilms on aerobic, and anaerobic dental microorganisms. Analyzes of cytotoxicity using MTT assay, and genotoxicity by micronucleus test were performed in human keratinocytes (HACAT), murine macrophages (RAW 264.7), and murine fibroblasts (L929). The anti-inflammatory effect was checked in RAW 264.7 stimulated by lipopolysaccharide (LPS) from Escherichia coli, and treated with the plant extract. The levels of cytokines, and nitric oxide (NO) were measured by ELISA, and Griess method, respectively. Data were analyzed by ANOVA, followed by Tukey's, or Kruskal-Wallis, and Dunns tests (P ≤ 0.05). Biofilms of anaerobic bacteria were very susceptible to the plant extract. Effective concentrations showed cell viability > 50%, except 25 mg/mL for HACAT after 24 h of exposure. The extract of S. adstringens was not genotoxic for RAW 264.7. LPS associated with extract increased the production of all cytokines, except TNF-α. However, the plant extract decreased the production of NO. In conclusion, the extract of S. adstringens affected biofilm of anaerobic bacteria using non-cytotoxic concentrations for RAW 264.7, L929, and HACAT cells. Stryphnodendron adstringens (Mart.) Coville es una planta medicinal conocida por sus propiedades antiinflamatorias y antimicrobianas. Este estudio evaluó algunas actividades biológicas del extracto de S. adstringens. La actividad antimicrobiana se verificó en cultivos planctónicos y biopelícula monomicrobianos en microorganismos aerobios y anaerobios de interés dental. Se realizaron análisis de citotoxicidad mediante ensayo MTT y genotoxicidad mediante ensayo de micronúcleos en queratinocitos humanos (HACAT), macrófagos murinos (RAW 264.7) y fibroblastos murinos (L929). El efecto antiinflamatorio se verificó en RAW 264.7 estimulado por lipopolisacárido (LPS) de Escherichia coli y tratado con el extracto vegetal. Los niveles de citocinas y óxido nítrico (NO) se midieron mediante ELISA y el método de Griess, respectivamente. Los datos se analizaron mediante ANOVA y prueba de Tukey o prueba de Kruskal-Wallis y Dunns (P ≤ 0,05). Las biopelículas de bacterias anaerobias fueron muy susceptibles al extracto de la planta. Las concentraciones efectivas mostraron viabilidad celular> 50%, excepto 25 mg/mL para HACAT después de 24 h de exposición. El extracto de S. adstringens no fue genotóxico para RAW 264.7. El LPS asociado con el extracto aumentó la producción de todas las citocinas, excepto TNF-α. Sin embargo, el extracto de la planta disminuyó la producción de NO. En conclusión, el extracto de S. adstringens afectó el biopelícula de bacterias anaerobias utilizando concentraciones no citotóxicas para RAW 264.7, L929 y HACAT. Stryphnodendron adstringens (Mart.) Coville é uma planta medicinal conhecida por suas propriedades anti-inflamatórias e antimicrobianas. Este estudo avaliou algumas atividades biológicas do extrato de S. adstringens. A atividade antimicrobiana foi verificada em culturas planctônicas e biofilmes monomicrobianos em microrganismos aeróbios e anaeróbios de interesse odontológico. Análises de citotoxicidade utilizando ensaio de MTT e genotoxicidade por meio do teste de micronúcleo foram realizadas em queratinócitos humanos (HACAT), macrófagos murinos (RAW 264.7) e fibroblastos murinos (L929). O efeito anti-inflamatório foi verificado em RAW 264.7 estimulado por lipopolissacarídeo (LPS) de Escherichia coli e tratado com o extrato da planta. Os níveis de citocinas e óxido nítrico (NO) foram medidos por ELISA e método de Griess, respectivamente. Os dados foram analisados ​​por ANOVA e teste de Tukey ou Kruskal-Wallis e teste de Dunns (P ≤ 0,05). Os biofilmes de bactérias anaeróbias foram muito suscetíveis ao extrato da planta. As concentrações efetivas mostraram viabilidade celular > 50%, exceto 25 mg/mL para HACAT após 24 h de exposição. O extrato de S. adstringens não foi genotóxico para RAW 264.7. O LPS associado ao extrato aumentou a produção de todas as citocinas, exceto TNF-α. No entanto, o extrato da planta diminuiu a produção de NO. Em conclusão, o extrato de S. adstringens afetou o biofilme de bactérias anaeróbias usando concentrações não citotóxicas para células RAW 264.7, L929, e HACAT.