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ObjectiveTo utilize whole genome sequencing to comprehensively compare and analyze the genomic characteristics of Listeria monocytogenes （L. monocytogenes） and Listeria innocua （L. innocua） isolated from commercially available foods in Gansu Province.MethodsDuring 2021 and 2022， 25 strains of L. monocytogenes and 7 strains of L. innocua were isolated from commercially available food sources within Gansu Province and subjected to whole genome sequencing for phylogenetic lineage， clonal complex （CC）， sequence type （ST）， virulence gene， resistance gene， and pan-genome analyses.ResultsThe 32 Listeria strains examined could be classified into three distinct groups： L. monocytogenes lineages Ⅰ and Ⅱ and L. innocua. Considering their CC types， further subdivision into 10 subgroups was observed for L. monocytogenes， whereas L. innocua had 5 subgroups. Core genome multi-locus sequence typing effectively differentiated strains with different CC types within each lineage， with lineage Ⅰ showing a closer evolutionary relationship with L. innocua. All 25 L. monocytogenes strains carried Listeria pathogenicity island 1 （LIPI-1） and internalin genes； none carried LIPI-3， and 2 strains of ST87 carried LIPI-4. Conversely， none of the seven strains of L. innocua carried LIPI-1 or internalin genes； however， they all carried LIPI-4， and 5 carried LIPI-3. Among the L. monocytogenes strains， 16 carried surface sensory island 1 （SSI-1）， and 3 carried SSI-2； all 7 L. innocua strains did not carry SSI-1， but 6 of them carried SSI-2. The pan-genome size of L. monocytogenes increased linearly with the number of sequenced genomes. Upon reaching 15 strains， the number of core genes stabilized at 2 272， accounting for 46.2% of the pan-genome genes. Furthermore， 1 487 common core genes were shared between the 25 L. monocytogenes strains and 7 L. innocua strains， with the count tending to stabilize when the strain count reached 10.ConclusionMulti-locus sequence typing of the core genome can effectively distinguish among Listeria strains originating from different lineages and CCs. The similarity in the biochemical characteristics of L. monocytogenes and L. innocua is due to their close genetic relationship， while the differences in their pathogenicity can be attributed to the presence of certain virulence genes in L. monocytogenes.

Since the last IAEA-FEC in 2021, significant progress on the development of long pulse steady state scenario and its related key physics and technologies have been achieved, including the reproducible 403 s long-pulse steady-state H-mode plasma with pure radio frequency (RF) power heating. A thousand-second time scale (∼1056 s) fully non-inductive plasma with high injected energy up to 1.73 GJ has also been achieved. The EAST operational regime of high βP has been significantly extended (H98y2 > 1.3, βP ∼ 4.0, βN ∼ 2.4 and ne/nGW ∼ 1.0) using RF and neutral beam injection (NBI). The full edge localized mode suppression using the n = 4 resonant magnetic perturbations has been achieved in ITER-like standard type-I ELMy H-mode plasmas with q95 ≈ 3.1 on EAST, extrapolating favorably to the ITER baseline scenario. The sustained large ELM control and stable partial detachment have been achieved with Ne seeding. The underlying physics of plasma-beta effect for error field penetration, where toroidal effect dominates, is disclosed by comparing the results in cylindrical theory and MARS-Q simulation in EAST. Breakdown and plasma initiation at low toroidal electric fields (

The sputtering and transport of tungsten (W) impurities in helium (He) and deuterium (D) plasma discharges are compared using the SOLPS-ITER code. To reduce the computational resources of modeling, W ions are treated using the bundled charge state model. The results show that the W erosion flux of He plasma is almost a factor of two higher than that of D plasma under the same upstream electron density and heating power due to the higher W sputtering yield in He plasma. Moreover, the W self-sputtering flux is significantly higher than the W flux sputtered by the main ions. The leakage and retention of W impurities in the divertor region is also analyzed. W ions mainly escape from the near scrape-off layer (SOL) region through the divertor entrance as the stagnation point of the average W impurity poloidal velocity is considerably closer to the target plates in the near SOL region. Furthermore, the leakage flux of W ions in He plasma is higher than that in D plasma, mainly because of the higher W sputtering level in He plasma, which results in a larger W density. W ions with low-lying charge states, mostly comprising the charge state of W10–12+, easily escape from the divertor through the near SOL flux tubes in both D and He plasmas. In addition, the effects of upstream electron density on W sputtering and retention in He and D plasma discharges are presented. [ABSTRACT FROM AUTHOR]

Chronic obstructive pulmonary disease (COPD) is a disease whose incidence increase with age and is characterised by chronic inflammation and significant immune dysregulation. Inhalation of toxic substances cause oxidative stress in the lung tissue as well as airway inflammation, under the recruitment of chemokines, immune cells gathered and are activated to play a defensive role. However, persistent inflammation damages the immune system and leads to immune dysregulation, which is mainly manifested in the reduction of the body's immune response to antigens, and immune cells function are impaired, further destroy the respiratory defensive system, leading to recurrent lower respiratory infections and progressive exacerbation of the disease, thus immune dysregulation play an important role in the pathogenesis of COPD. This review summarizes the changes of innate and adaptive immune-related cells during the pathogenesis of COPD, aiming to control COPD airway inflammation and improve lung tissue remodelling by regulating immune dysregulation, for further reducing the risk of COPD progression and opening new avenues of therapeutic intervention in COPD. [ABSTRACT FROM AUTHOR]

Radiative divertor is an effective method for the divertor heat flux control, but excessive core impurity radiation can deteriorate the confinement in tokamaks. In recent EAST experiments, the compatibility of divertor detachment and impurity control with good core confinement (H98,y2 ∼1) has been achieved simultaneously by combining an upstream deuterium (D2) puff and divertor argon (Ar) seeding. Both experimental and SOLPS-ITER modeling reveal that additional D2 puff can further mitigate the target heat load and facilitate the detachment. The SOLPS-ITER results also show that the D2 puff makes a limited contribution to the Ar line radiation but a significant contribution to the neutral radiation in the SOL and divertor regions. The physical mechanism behind the effect of the D2 puff on the Ar retention is also revealed by the modeling. The increase in the Ar velocity is the major reason for the improved Ar retention in the partial detachment state. The increase in vAr is mainly caused by the strengthening of frictional drag of D+, which is because the additional D2 puff increases the pressure gradient force (FPG) and the electric field force (FE) on D+. Further analysis reveals that in the deep detachment state, some other negative effects play a dominant role in determining the Ar retention, such as the shift of the region of the Ar+ ionization source, the decrease in the strength of the Ar+ ionization source, and the net force on the Ar ions near the target. [ABSTRACT FROM AUTHOR]

In order to address the greenhouse effect and achieve the artificial carbon cycle, the electrocatalytic reduction reactions offer an effective approach for converting carbon dioxide (CO2) into valuable chemicals. In addition to the rational design of catalyst structure to improve the high selective conversion of CO2, exploring the influence of environmental conditions on the reaction path and product types is one of the keys to realizing the high selective conversion of CO2. In this review, we illustrate the important influence of changes in environmental conditions on the effectiveness of electrocatalytic carbon dioxide reduction reaction (CO2RR) technology from the design of the reactor, the selection of the electrolyte, and the setting of the reaction conditions (pressure, temperature, etc.). Through in‐depth understanding and optimization of these factors, the efficiency and selectivity of the CO2RR can be further improved to promote the development of CO2 conversion technology. [ABSTRACT FROM AUTHOR]

Sensitively monitoring metallothionein (MT), a heavy metal-binding protein with substantial cysteine content, is of significance for evaluating heavy metal poisoning in both humans and animals. Based on a new metal ion-coordinated DNA probe and the heavy metal ion binding capability of MT, as well as the substantial signal enhancement of the hybridization chain reaction (HCR) and rolling circle amplification (RCA), we demonstrate a highly sensitive fluorescence MT detection assay. MT binds the metal ions in the hairpin structured, metal ion-coordinated DNA probe to switch its hairpin structure into ssDNA, which triggers subsequent RCA reactions and HCRs to open plenty of fluorescently quenched signal hairpins to exhibit drastically amplified fluorescence recovery for assaying MT down to 0.58 nM within a dynamic range of 1–320 nM. In addition, the investigation of low contents of MT in diluted human serum by such an assay has also been verified, indicating its promising application potential for diagnosing heavy metal poisoning. [ABSTRACT FROM AUTHOR]