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Objective To investigate the effect of VRK1 expression downregulated by siRNA on BANF1 expression and the proliferation and migration abilities of esophageal squamous cell carcinoma (ESCC) cells. Methods VRK1 siRNA was transfected into EC109 and EC1 cell lines to interfere VRK1 expression. Western blot was used to detect the interference efficiency of VRK1 siRNA and BANF1 expression in esophageal carcinoma cells after siRNA interference. CCK-8 and flow cytometry were performed to detect the effect of VRK1 expression on cell proliferation. Transwell assay was used to observe the changes of cell migration ability. Results After VRK1 siRNA transfection, VRK1 expression were down-regulated for 62.40% and 52.14%, and BANF1 protein expression were down-regulated significantly for 24.51% and 52.87% in EC109 and EC1 cell lines, respectively. The proliferation of EC109 and EC1 cell lines were decreased 12h after transfection, with the highest inhibition rate at 36h(19.41% and 20.10%). The cells percentages in G2 and G1 phases were decreased while that in S phase was increased after down-regulation of VRK1 and BANF1 expression. The number of cell migration was decreased in the experimental group, compared with control group (P < 0.01). Conclusion Down-regulation of VRK1 expression by siRNA could inhibit the proliferation and migration abilities of esophageal squamous carcinoma cells, which may be achieved by down-regulating BANF1 expression.

BACKGROUND: Preventable medical errors in hospital settings are the third leading cause of deaths in the United States. However, less is known about harm that occurs in patients in outpatient settings, where the majority of care is delivered. We do not know the likelihood that a patient sitting in a dentist chair will experience harm. Additionally, we do not know if patients of certain race, age, sex, or socioeconomic status disproportionately experience iatrogenic harm. METHODS: We initiated the Dental Practice Study (DPS) with the aim of determining the frequency and types of adverse events (AEs) that occur in dentistry on the basis of retrospective chart audit. This article discusses the 6-month pilot phase of the DPS during which we explored the feasibility and efficiency of our multistaged review process to detect AEs. RESULTS: At sites 1, 2, and 3, respectively, 2 reviewers abstracted 21, 11, and 23 probable AEs, respectively, from the 100 patient charts audited per site. At site 2, a third reviewer audited the same 100 charts and found only 1 additional probable AE. Of the total 56 probable AEs (from 300 charts), the expert panel confirmed 9 AE cases. This equals 3 AEs per 100 patients per year. Patients who experienced an AE tended to be male and older and to have undergone more procedures within the study year. CONCLUSIONS: This article presents an overview of the DPS. It describes the methods used and summarizes the results of its pilot phase. To minimize threats to dental patient safety, a starting point is to understand their basic epidemiology, both in terms of their frequency and the extent to which they affect different populations.

This study aims to investigate burnout and study engagement among medical students at Sun Yat-sen University, China.A cross-sectional survey was conducted among undergraduate medical students of Sun Yat-sen University, China. A total of 453 undergraduate students completed a self-administered, structured questionnaire between January and February, 2016. Burnout and study engagement were measured using the Maslach Burnout Inventory-Student Survey (MBI-SS) and the UTRECHT Work Engagement Scale-Students (UWES-S), respectively. Subjects who scored high in emotional exhaustion subscale, high in cynicism subscale, and low in professional efficacy subscale simultaneously were graded as having high risk of burnout. Independent sample t tests and chi-square tests were used to compare the differences in burnout and work engagement between genders, majors, and grade levels.The means (standard deviations) of the MBI-SS subscales were 3.42 (1.45) for emotional exhaustion, 2.34 (1.64) for cynicism, and 3.04 (1.30) for professional efficacy. The means (standard deviations) of the UWES-S subscales were 3.13 (1.49) for vigor, 3.44 (1.47) for dedication and 3.00 (1.51) for absorption. Approximately 1 in 11 students experienced a high risk of burnout. There were no statistically significant gender differences in burnout and study engagement. There were also no statistically significant differences in burnout and study engagement subscales according to student major. Students in higher grades displayed increased burnout risk, higher mean burnout subscale score of cynicism, lower mean burnout subscale score of professional efficacy, and decreased mean study engagement subscale scores of dedication and absorption. There were strong correlations within study engagement subscales.Chinese medical students in this university experience a high level of burnout. Students at higher-grade level experience more burnout and decreased study engagement compared with students in lower level.