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Background: Lipid‐injectable emulsions (ILEs) are a necessity for neonates dependent on parenteral nutrition (PN). In this manuscript, we describe the patterns of ILE use in neonatal intensive care units (NICUs) in the United States (US). Methods: An electronic survey was sent to 488 NICUs across the US between December 2020 and March 2021. Survey fields included availability and utilization of various ILE in neonates. Results: The response rate was 22% (107 out of 488). Soybean oil ILE (SO‐ILE) and soybean oil, medium‐chain triglycerides, olive oil, fish oil ILE (SO, MCT, OO, FO‐ILE) had similar availability (87% vs 86%, respectively), and SO, MCT, OO, FO‐ILE was more commonly used (SO‐ILE, 71% vs SO, MCT, OO, FO‐ILE, 86%). Fish oil‐ILE (FO‐ILE) was used by 55% of centers. SO‐ILE was most frequently used with PN and needs <4 weeks without cholestasis (79%). The most common reason for SO, MCT, OO, FO‐ILE use was cholestasis (71%). ILE minimization was used by 28% of SO‐ILE and 22% of SO, MCT, OO, FO‐ILE users; 95% of these centers restrict SO, MCT, OO, FO‐ILE to doses ≤2 g/kg/day. Twenty‐two percent of centers started FO‐ILE at direct bilirubin of >5 mg/dl. Conclusion: The results of this survey reveal significant variability in ILE usage across the US. Lipid minimization with SO, MCT, OO, FO‐ILE and initiation of FO‐ILE for cholestasis at higher bilirubin thresholds are prevalent. Such reports are crucial for a better understanding of ILE use in the NICU and in future ILE development. [ABSTRACT FROM AUTHOR]

Copyright of Bioscience Journal is the property of Bioscience Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Bioscience Journal is the property of Bioscience Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Bioscience Journal is the property of Bioscience Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Animals have evolved foraging strategies to acquire blends of nutrients that maximize fitness traits. In social insects, nutrient regulation is complicated by the fact that few individuals, the foragers, must address the divergent nutritional needs of all colony members simultaneously, including other workers, the reproductives, and the brood. Here we used 3D nutritional geometry design to examine how bumblebee workers regulate their collection of 3 major macronutrients in the presence and absence of brood. We provided small colonies artificial nectars (liquid diets) and pollens (solid diets) varying in their compositions of proteins, lipids, and carbohydrates during 2 weeks. Colonies given a choice between nutritionally complementary diets self-selected foods to reach a target ratio of 71% proteins, 6% carbohydrates, and 23% lipids, irrespective of the presence of brood. When confined to a single nutritionally imbalanced solid diet, colonies without brood regulated lipid collection and over-collected protein relative to this target ratio, whereas colonies with brood regulated both lipid and protein collection. This brood effect on the regulation of nutrient collection by workers suggests that protein levels are critical for larval development. Our results highlight the importance of considering bee nutrition as a multidimensional phenomenon to better assess the effects of environmental impoverishment and malnutrition on population declines. [ABSTRACT FROM AUTHOR]

Energy efficiencies and maintenance parameters have been traditionally estimated with a linear regression model that treated metabolizable energy intake as the dependent variable and protein and lipid depositions as the independent variables. Several studies have described the statistical issues associated with this approach, such as the reverse role of dependent and independent variables and a potential multicollinearity issue due to the high correlation between protein and lipid depositions. Biased regression techniques have been proposed to minimize the harmful effects of multicollinearity on the estimates of energy efficiencies. These approaches, however, only partially addressed the issues described for the linear regression approach. A first multivariate approach was developed by L. J. Koong in the 1970s, who estimated the energy parameters using a set of simultaneous equations. This multivariate approach has been considerably extended in the past two decades with the complete characterization of model's biological interpretation under different feeding conditions, the simultaneous estimation of maintenance requirements, the extension of the model to a mixed-effects framework, and the implementation of a Bayesian framework for model fitting. The multivariate approach has been successfully applied to model energy deposition and partitioning by mice, pigs, salmon, and rainbow trout. However, multivariate models are, in general, harder to fit than linear regression models due to 1) larger number of parameters, 2) issues with parameter identifiability, and 3) overall lack of algorithm convergence. Therefore, with the recent availability of easy to use and efficient computer packages for model fitting, the use of a Bayesian framework seems to be an attractive approach for fitting multivariate models describing protein and lipid deposition. [ABSTRACT FROM AUTHOR]

Highlights • Seven types of wheat and rye flours were characterized. • Flours presented moisture and ash contents below the recommended maximum limit. • Low lipid content was observed avoiding changes in flours' smell and taste. • The microorganisms' counting was not significantly different in both refined samples. • Micotoxins, aflatoxins and ochratoxin A were not detected in any of the flours. Abstract Seven types of wheat and rye flours were studied regarding their physical and chemical properties, as well as the presence of mycotoxins and microorganisms. The results revealed that flours presented moisture and ash contents below the recommended maximum limit. They also presented a low lipid content, which helps avoiding changes in the flours' smell and taste. From the microbiological analysis, comparing the refined rye and wheat flours, the counting in the analysed microorganisms was not significantly different. The whole flours presented high contents in almost all the tested microorganisms, highlighting the molds counting for the whole wheat flour and the aerobic plate counting for the whole rye flour. None of the samples presented Salmonella spp. Aflatoxins and ochratoxin A were not detected in any of the flours. To the best of our knowledge, this is the first characterization of wheat and rye flours for Portuguese consumers. [ABSTRACT FROM AUTHOR]

Graphical abstract Highlights • Fatty acid species and locations within TAGs affect lipid digestion rate and extent. • Maximum FFA release extent upon pancreatic lipase reaction: PO > RO > LINO > SO > LO. • Apparent FFA release constants were PO > SO ≈ RO > LO ≈ LINO during in-vitro digestion. • Short-chain saturated fatty acids released faster than long-chain poly-unsaturated fatty acids. • Correlations were found between lipid compositions, lipid digestion and serum fatty acids profiles. Abstract The objective of the present study was to investigate the influences of dietary lipid composition on the gastrointestinal digestion and postprandial serum lipid profiles, and the connections between them. The in-vitro digestion results showed that maximum free fatty acid (FFA) release level of different lipid samples was PO (Palm oil) > RO (Rapeseed oil) > LINO (Linseed oil) > SO (Sunflower oil) > LO (Lard oil), and the first-order kinetics apparent rate constant was PO > SO ≈ RO > LO ≈ LINO, this may probably be ascribed to their specific lipid fatty acid composition and TAG structure. The individual FFA released during 240 min in-vitro digestion time was measured, and it showed that the release rate of short-chain saturated fatty acids (e.g. C16:0 in PO) were higher than the long-chain poly-unsaturated fatty acids (e.g. C18:3n-3 in LINO). Besides, the position of fatty acids within TAG molecules could also impose influences on the lipid hydrolysis process upon pancreas lipase in gastrointestinal tract using in-vitro digestion model. The postprandial serum fatty acid composition of the adult SD male rats were examined within 240 min after oral gavage administration, and the Pearson correlations between lipid fatty acid composition and the serum fatty acid profiles were analyzed. Certain correlations were summarized between lipid compositions (i.e. fatty acid composition and TAG structure), lipid digestion fates and serum fatty acid content in postprandial. The present work may provide some basic understandings of the connections among lipid compositions, lipid gastrointestinal digestion differences and the postprandial serum lipid profiles, and provide useful information about their nutritional and functional evaluation. [ABSTRACT FROM AUTHOR]

Background: In the International Lipid-Based Nutrient Supplements (iLiNS)-DYAD-Ghana trial, prenatal small-quantity lipid-based nutrient supplements (LNSs) had a positive effect on birth weight. Birth weight may be inversely related to blood pressure (BP) later in life.Objectives: We examined the effect of the intervention on BP at 4-6 y of age, and maternal and child factors related to BP.Methods: The iLiNS-DYAD-Ghana study was a partially double-blind, randomized controlled trial which assigned women (n = 1320) ≤20 weeks of gestation to daily supplementation with: 1) iron and folic acid during pregnancy and 200 mg Ca for 6 mo postpartum , 2) multiple micronutrients during pregnancy and postpartum, or 3) LNSs during pregnancy and postpartum plus LNSs for infants from 6 to 18 mo of age. At 4-6 y of age (n = 858, 70% of live births), we compared BP, a secondary outcome, between non-LNS and LNS groups and examined whether BP was related to several factors including maternal BP, child weight-for-age z score (WAZ), and physical activity.Results: Non-LNS and LNS groups did not differ in systolic (99.2 ± 0.4 compared with 98.5 ± 0.6 mm Hg; P = 0.317) or diastolic (60.1 ± 0.3 compared with 60.0 ± 0.4 mm Hg; P = 0.805) BP, or prevalence of high BP (systolic or diastolic BP ≥90th percentile of the US National Heart, Lung, and Blood Institute reference: 31% compared with 28%; P = 0.251). BP at 4-6 y of age was positively related to birth weight; this relation was largely mediated through concurrent WAZ in a path model. Concurrent WAZ and maternal BP were the factors most strongly related to child BP.Conclusions: Despite greater birth weight in the LNS group, there was no intervention group difference in BP at 4-6 y. In this preschool population at high risk of adult hypertension based on BP at 4-6 y, high maternal BP and child WAZ were key factors related to BP. This trial was registered at clinicaltrials.gov as NCT00970866. [ABSTRACT FROM AUTHOR]

Background: Whether consuming sweet foods early in life affects sweet food preferences and consumption later in childhood is unknown.Objective: We tested the hypothesis that exposure to a slightly sweet lipid-based nutrient supplement (LNS) early in life would not increase preference for or consumption of sweet items at preschool age.Methods: We followed up children who had participated in a randomized trial in Ghana in which LNS was provided to 1 group of women during pregnancy and 6 mo postpartum and to their infants from ages 6-18 mo (LNS group). The control group (non-LNS group) received iron and folic acid during pregnancy or multiple micronutrients during pregnancy and 6 mo postpartum, with no infant supplementation. At 4-6 y, we obtained data from caregivers on children's food and beverage preferences and consumption (n = 985). For a randomly selected subsample (n = 624), we assessed preference for sweet items using a photo game (range in potential scores, 0-15). For the photo game and reported consumption of sweet items, we examined group differences using predetermined noninferiority margins equivalent to an effect size of 0.2.Results: Median (quartile 1, quartile 3) reported consumption of sweet items (times in previous week) was 14 (8, 23) in the LNS group and 16 (9, 22) in the non-LNS group; in the photo game, the number of sweet items selected was 15 (11, 15) and 15 (11, 15), respectively. The upper level of the 95% CI of the mean difference between LNS and non-LNS groups did not exceed the noninferiority margins for these outcomes. Caregiver-reported preferences for sweet items also did not differ between groups (P = 0.9).Conclusion: In this setting, where child consumption of sweet foods was common, exposure to a slightly sweet LNS early in life did not increase preference for or consumption of sweet foods and beverages at preschool age. This trial was registered at clinicaltrials.gov as NCT00970866. [ABSTRACT FROM AUTHOR]

Background Lipid droplets in human milk have a mode diameter of ∼4 μm and are surrounded by a native phospholipid-rich membrane. Current infant milk formulas (IMFs) contain small lipid droplets (mode diameter ∼0.5 μm) primarily coated by proteins. A concept IMF was developed mimicking more closely the structure and composition of human milk lipid droplets. Objectives This randomized, controlled, double-blind equivalence trial evaluates the safety and tolerance of a concept IMF with large, milk phospholipid–coated lipid droplets (mode diameter 3–5 μm) containing vegetable and dairy lipids in healthy, term infants. Methods Fully formula-fed infants were enrolled up to 35 d of age and randomly assigned to 1 of 2 formulas until 17 wk of age: 1) Control IMF with small lipid droplets containing vegetable oils (n  = 108); or 2) Concept IMF with large, milk phospholipid–coated lipid droplets comprised of 48% dairy lipids (n  = 115). A group of 88 breastfed infants served as reference. Primary outcome was daily weight gain during intervention. Additionally, number and type of adverse events, growth, and tolerance parameters were monitored. Results Equivalence of daily weight gain was demonstrated (Concept compared with Control IMF: −1.37 g/d; 90% CI: −2.71, −0.02; equivalence margin ± 3 g/d). No relevant group differences were observed in growth, tolerance and number, severity, or relatedness of adverse events. We did observe a higher prevalence of watery stools in the Concept than in the Control IMF group between 5 and 12 wk of age (P  < 0.001), closer to the stool characteristics observed in the breastfed group. Conclusions An infant formula with large, milk phospholipid–coated lipid droplets containing dairy lipids is safe, well tolerated, and supports an adequate growth in healthy infants. This trial was registered in the Dutch Trial Register (www.trialregister.nl) as NTR3683. [ABSTRACT FROM AUTHOR]

Abstract Dietary supplementation of vitamin E (VitE) in a synthetic or natural form was examined. Forty-eight lambs were assigned (n = 16) to either a grain-based diet at moderate (MOD, 42 mg∙kg−1 VitE as all-rac α-tocopheryl acetate) or supranutritional (SUP, 285 mg∙kg−1 of vitE) levels of synthetic VitE or a lucerne hay-based diet (LUC; 37 mg∙kg−1 VitE) for 8 weeks. Meat from the LUC group had lower muscle n-6 and PUFA levels compared to meat from the MOD and SUP groups. Despite a similar VitE intake, muscle VitE was higher for LUC compared to MOD, while SUP lambs showed the highest VitE. Lipid oxidation did not differ between groups. For fresh meat, redness tended to be higher in LUC fed lambs than the other two groups, but brownness formation was only lower than the SUP group. For aged meat colour stability, redness tended to be higher in lambs fed SUP and LUC, whereas highest browning occurred in the MOD group. [ABSTRACT FROM AUTHOR]

The red-legged partridge (Alectoris rufa) is a feathered game species of great socioeconomic importance in its native range and also in the UK. The aim of this study was to present a detailed comparison of meat's lipid fraction obtained from wild and farm-raised specimens and simultaneously compare the breast and leg meat portions. Meat from wild specimens had a significant (P < 0.05) lower proportion of saturated fatty acid (less 5.1%) and presented better P/S and n-6/n-3 ratios, and atherogenicity index than farm-raised counterparts. The wild specimens presented significant (P < 0.001) higher contents of total vitamin E (8.8 vs. 2.2 μg/g of fresh meat), is for that reason less prone to lipid peroxidation than farm-raised specimens. Meat portions differed significantly (P < 0.05) on total lipid and total cholesterol contents and in all partial sums of fatty acids. The breast was leaner (0.86 vs. 1.47 g/100 g of meat), with lower total cholesterol (37.5 vs. 54.7 mg/100 g of meat), lower saturated fatty acid, monounsaturated fatty acid, polyunsaturated fatty acid, and n-6 polyunsaturated fatty acid (less 0.27, 0.28, 0.10, and 0.11 g/100 g of fresh meat, correspondingly). Regarding the fatty acid ratios and lipid quality indexes, breast meat presents better n-6/n-3 ratio and atherogenicity and thrombogenicity indexes. [ABSTRACT FROM AUTHOR]

Abstract Silver barb (Puntius gonionotus) is considered as a promising medium-sized carp species for freshwater aquaculture in Asia. This study in silver barb was carried out to evaluate the effects of increasing dietary levels of lipid on growth, nutrient utilization, whole-body composition, tissue fatty acid composition and Δ6 fatty acyl desaturase (Δ6 fad) gene expression. Fish (11.3 ± 0.23 g of initial body weight) was fed for 60 days with five experimental diets: FO-0 (control feed); FO-30; FO-60; FO-90 and FO-120 containing 0, 30, 60, 90 and 120 g fish oil kg−1 diet, respectively. Among the diets, the highest specific growth rate (SGR), protein efficiency ratio (PER) and whole-body lipid content, and the lowest feed conversion ratio (FCR) were recorded with FO-120 diet. The saturated fatty acids (SFA) level in the muscle was significantly (P <.05) increased with the enhanced FO supplementation, whereas monounsaturated fatty acids (MUFA) level decreased. Increased level of fish oil in the diet also enhanced the n-3 PUFA and n-3 LC-PUFA (long-chain polyunsaturated fatty acid) in the muscle and liver. The expression of Δ6 fad gene was downregulated, whereas the serum biochemical constituents were either remain unchanged or enhanced with increased FO supplementation in the diets of silver barb. [ABSTRACT FROM AUTHOR]

Abstract: This study was carried out to assess the effects of a blend of herbal essential oils (namely Thymus vulgaris, Mentha piperita, Rosmarinus offisinalis and Anethum graveolens) and different dietary lipid sources on the performance, ileal bacterial enumeration, intestinal histological alterations and immune responses in laying hens. For this purpose, a total of 150 laying hens were randomly allocated to six experimental treatments with five replicates of five birds each. Dietary treatments consisted of three levels of a mixture of essential oils (0, 100 and 200 mg/kg) and two sources of lipid (soybean oil and fish oil containing different ratios of n‐6 to n‐3 fatty acids) fed to the animals during an 80‐days feeding trial. Findings indicated that dietary administration of fish oil not only increased significantly the spleen relative weight (p < 0.01) and the antibody titre against Newcastle virus (p < 0.05) but also led to reductions in liver relative weight (p < 0.05) and feed conversion ratio (p < 0.05). Moreover, the mixture of herbal essential oils brought about declines in hepatic relative weight, heterophile to lymphocyte ratio (p < 0.05) and intestinal pathogen populations (p < 0.01). Mention must also be made of the improvements it made in spleen weight (p < 0.01), antibody titres against SRBC (p < 0.01) and Newcastle virus (p < 0.05), villus height to crypt depth ratio (p < 0.01), goblet cell numbers (p < 0.05), lamina propria lymphatic follicle diameters (p < 0.01) and feed conversion ratio (p = 0.06). It may be claimed that the enhancements observed in the performance of laying hens fed fish oil and 200 mg/kg of the blend of essential oils could have potentially been associated with improved intestinal health indices as well as cellular and humoral immune responses. [ABSTRACT FROM AUTHOR]

Intestinal villi of Caiman yacare form longitudinal folds instead of the finger-like projections of most birds and mammals. Moreover, they lack Crypts of Lieberkühn and the lamina epithelialis organization is dynamic, changing from pseudostratified to simple columnar epithelium after feeding. Because of these differences, we sought to verify whether intestinal villi of the crocodilian Caiman yacare are functionally compartmentalized along their length similarly to the finger-like villi that harbors Crypt of Lieberkühn. For this, Caiman yacare were force-fed soybean oil, the intestinal mucosa was harvested and analyzed under light microscopy after lipid staining or immunohistochemistry for the proliferative marker PCNA. Functional compartmentalization was assessed by evaluating differences in lipid absorption along intestinal villi base-to-tip axis, by localizing the proliferative enterocytes and by verifying whether such cells were capable of absorbing lipids. Histological morphometric analyses of the extent of enterocyte hypertrophy caused by lipid inclusions and the contribution of such inclusions to histological remodeling from pseudostratified to simple columnar epithelium were also evaluated. Although lacking Crypts of Lieberkühn, enterocytes present at villi base were PCNA positive and devoid of the great amount of lipid inclusions observed in the other intestinal villi domains, in a similar pattern to finger-like villi. Enterocytes doubled their volume because of lipid inclusions, and in spite of such enterocyte hypertrophy, lamina epithelialis continued to be pseudostratified within lateral sides, whereas villi tip were organized in a simple columnar epithelium. [ABSTRACT FROM AUTHOR]

Olive leaves are a waste of the olive oil processing industry and represent a good source of phenolic compounds. The aim of this work was to assess the influence of olive leaf extract (OLE) on lipid oxidation of baked snacks, like breadsticks, made with wheat flour, extra virgin olive oil (EVO), white wine, and salt. Two EVOs having different peroxide value and antioxidant profile (total phenol content, tocopherols, carotenoids, and antioxidant activity) were considered. The snacks were subjected to oven test or stored in the usual conditions of retailer shelves. The obtained data highlighted that EVO plays a key role both for the quality and for the shelf-life of baked snacks and the use of OLE is recommended especially when baked snacks are produced with low quality EVO which therefore does not have a good content of natural antioxidants. The OLE addition significantly reduced the forced oxidative degradation during oven test, as evidenced by a decrease of 27% in oxidation-related volatile compounds and of 42% in triacylglycerol oligopolymers compared to control snacks (CTR) without OLE. Moreover, OLE effectively acted also in normal storage conditions, improving sensory data, induction times, antioxidant activity, and volatile compounds compared to CTR (i.e. hexanal 165.49 vs 38.31 μg g −1 in OLE-added). The amount of oxidation-related volatile compounds showed an opposite trend with the quality level of oil used. [ABSTRACT FROM AUTHOR]

Abstract: The genetic improvement of redlip mullet Liza haematocheila through breeding programmes is of interest for this important aquaculture species. Lipoprotein lipase (LPL) is a key enzyme in lipid deposition and metabolism. The purpose of this study was to investigate the nutritional regulation of LPL in redlip mullet. We cloned and identified the LPL gene, determined LPL gene expression in various tissues, and examined the effect of dietary lipid level on hepatic LPL gene expression. The LPL gene of redlip mullet Liza haematocheila (L.hLPL) was 2,395 bp in length and encoded 516 amino acids. Sequence analysis showed that L.hLPL shared 61%–90.3% identity with LPLs in other species. Expression patterns of hepatic L.hLPL were studied in redlip mullet fed diets containing 2.0, 4.8, 7.5, 9.8, 12.0 or 14.6 g/kg, crude fat for 60 days by real‐time quantitative PCR. The abundance of LPL mRNA in hepatic tissue increased with the increase in dietary fat. The expression L.hLPL mRNA was significantly higher in the groups fed diets with 14.6 and 12.0 g/kg fat than in the other groups (p < .05). Gene expression was significantly higher in the abdominal fat of redlip mullet (p < .05) compared with other tissues. In conclusion, a high fat diet (9.79–14.59 g/kg) induces L.hLPL expression in abdominal fat. [ABSTRACT FROM AUTHOR]

Abstract: Six isoproteic diets were designated to evaluate the effects of dietary lipid levels (from 70 to 270 g/kg) on the growth performance, feed utilization, digestive tract enzyme activity and lipid deposition of juvenile Brachymystax lenok (average initial weight 0.54 ± 0.04 g). Each diet was fed to triplicate tanks (30 fish per tank) in an indoor closed recirculating system for 9 weeks. Final body weight and weight gain were highest in fish fed 190 g/kg diet and lowest in fish fed the 70 g/kg diet. Specific growth rate of fish fed with 190 g/kg diet was significantly higher than those fed with 70 and 270 g/kg diets (p < .05). Protein efficiency ratio of fish fed with 70 g/kg diet was significantly lower than the 110–230 g/kg treatments and was not significantly different from the 270 g/kg treatment. Fish fed with 270 g/kg diet had significantly higher hepatosomatic index and viscerosomatic index than those fed with 70–190 g/kg diets (p < .05). Intraperitoneal fat ratio and the whole‐body lipid content had a trend to increase with increase in dietary lipid level. Muscle crude lipid content increased up to 190 g/kg with increase in dietary lipid level. Lipid retention decreased with increase in dietary lipid level, while no significant differences in protein intake and retention levels were observed in fish among all treatments. Lipase activity of the mixture of pyloric caeca and foregut in fish fed 190 and 230 g/kg diets was significantly higher than those fed 70 and 110 g/kg diets. Midgut and hindgut lipase activities of fish were significantly higher than those fed the 190 and 230 g/kg diets. In conclusion, based on the second‐order polynomial model of WG and FCR, this study suggested that 173.8–195.0 g/kg dietary lipid levels were appropriated for B. lenok. [ABSTRACT FROM AUTHOR]

The present study was conducted to investigate the effects of dietary DHA/EPA ratio on lipid accumulation in farmed fish, with an 80-day feeding trial on tongue sole Cynoglossus semilaevis , a typical marine flatfish. Six experimental diets differing basically in DHA/EPA ratio, i.e., 0.61, 1.02, 1.46, 1.91, 2.32, and 2.75, were randomly assigned to 18 tanks of juvenile tongue sole. Fish were reared in a flow-through seawater system and fed to apparent satiation twice daily. Compared to very low (0.61) or high (2.32 and 2.75) dietary DHA/EPA levels, moderate dietary DHA/EPA ratios of 1.02 and 1.46 induced higher lipid accumulation in fish flesh and whole fish. Although also inducing increased liver lipid accumulation, the moderate dietary DHA/EPA ratios did not compromise the fish health in terms of growth performances and hematological parameters. The fatty acid profile of fish tissues reflected closely those of the diets, but DHA had more efficient deposition in fish flesh than EPA. Excess EPA or DHA in diets down-regulated the mRNA expression of lipogenic genes in fish tissues, and DHA seemed to be more efficient than EPA in up-regulating gene expression of β-oxidation-related proteins. Both esterification and hydrolyzation of triglycerides in fish tissues seemed to be more active in groups with intermediate DHA/EPA ratios (1.02 and 1.46). The present study provided a new way to modulate the nutritive quality of farmed fish, as well as to make better use of precious LC-PUFA resources. [ABSTRACT FROM AUTHOR]

Objective: The aim of this study was to evaluate the effects of dietary intake of fiber present in banana peel and stalk on the blood glucose and lipid profiles of Wistar rats. Methodology: This research was conducted for 65 days to study the influence of diets containing fiber from banana peel (PGF), banana stalks (SFG) and both types of fiber (PSFG), as compared with the control diet (CG), on the glycemic and lipid profile of 24 male Wistar rats, with a mean initial weight of 54.06 g, distributed equally into four groups. The control group (CG) received the AIN diet plus 1% crystalline cholesterol and 0.1% colic acid and the experimental diets were similar to the control group, except for substitution of the fiber source. Results: In the treatment groups (PFG, SFG and PSFG), there was an increase in glycemic, triglyceride and serum total cholesterol and LDL-C levels compared with the control group (CG). The levels of VLDL-C were reduced only in the group containing fibers from the peel. Conclusions: Inclusion of dietary fiber from banana peels and stalks, as well as the mixture of both types of fiber, showed effectiveness of use on the lipid profile and the ability to maintain the blood glucose levels of rats fed on diets containing fiber from the peel and the stalk. [ABSTRACT FROM AUTHOR]

A feeding trial was designed to assess the effects of dietary protein and lipid content on growth, feed utilization efficiency, body composition, and hematological indices of juvenile filefish, Stephanolepis cirrhifer. Eight experimental diets were formulated with a combination of four protein (35, 40, 45, and 50%) and two dietary lipid levels (7 and 14%). Each diet was fed to triplicate groups of fish (3.2 ± 0.06 g) to apparent satiation for 8 wk. Fish growth performance and feed utilization were significantly affected by increasing dietary protein and lipid levels, with no significant interactions between factors. The highest growth performance value, in terms of weight gain, was observed in groups fed the diets with 50% protein (399%). However, fish fed the diet containing 45% protein had comparable growth (357%) while achieving relatively higher protein efficiency ratio. Hepatosomatic index was significantly affected by interaction of dietary lipid and protein with the highest values observed in those fish fed the highest protein (45-50%) and lipid (14%) diets. There was a significant increase in body lipid content (5.1 to 6.6%) and a decrease in body protein (15.8 to 14.8%) and ash (2.47 to 2.16%) with increasing dietary lipid levels from 7 to 14%. Muscle lipid content was significantly affected by both dietary protein and lipid levels and tended to increase with increasing dietary protein and lipid levels, ranging from 0.13 to 1.20%. Liver lipid content (65.9 to 68.7%) was significantly increased with the increase in dietary lipid levels while liver moisture content (28.9 to 25.9%) showed a clear decreasing trend. Hematological values were also altered with the increase in either dietary protein or lipid levels. These findings may suggest that a diet containing 45% protein and 7% lipid, with a protein to energy ratio of 23.8 mg/ kJ, could deliver sufficient nutrient and energy to support acceptable growth and feed utilization and avoid excessive fat deposition in juvenile filefish. [ABSTRACT FROM AUTHOR]

The clamworm Perinereis aibuhitensis is a commercially important polychaete in China, but knowledge about the nutritional demands of this species is limited. In this study, the effects of five lipid sources in the diet, namely fish oil ( FO), soyabean oil ( SO), rapeseed oil ( RO), cottonseed oil ( CO) and mixed vegetable oil ( MO), on growth, whole-body composition and antioxidant parameters of juvenile P. aibuhitensis were evaluated. The results showed that clamworms fed the CO diet had higher specific growth rate ( SGR) and protein efficiency ratio ( PER) than the other treatments. The accumulation of longer-chain polyunsaturated fatty acids ( PUFAs) was observed in P. aibuhitensis, suggesting that P. aibuhitensis had the ability to elongate and desaturate PUFAs with 18C to form longer-chain PUFAs. The values of n-3/n-6 in clamworms fed vegetable oil diets (ranged from 0.20 to 0.31) were much closer to the recommended values for human food compared with FO diet (2.47). Analysis of the antioxidant parameters revealed that clamworms fed the CO diet suffered lower peroxidation burden than those fed FO diet. These results suggested that cottonseed oil is a suitable lipid source for P. aibuhitensis feeds. [ABSTRACT FROM AUTHOR]

As the major non-protein energy sources, high carbohydrate (CHO) and lipid (L) diets have been widely used in aquaculture to reduce cost, but they also caused excessive lipid accumulation in the fish liver. It is not yet clear which, lipid or carbohydrate, is a greater influence on hepatic lipid accumulation. To study the effects of dietary carbohydrate and lipid levels on hepatic lipid deposition in Nile tilapia ( Oreochromis niloticus ), six diets with different carbohydrate and lipid levels (carbohydrate level ranged from 11.2% to 54.24%, while lipid level ranged from 21.51% to 2.70%, namely, the dietary CHO:L ratios ranged from 0.52:1 to 20.09:1) were formulated and fed to tilapia for 8 weeks. After the feeding trial, high specific growth rate (SGR) was observed in the groups with the CHO:L ratio of 1.95, 3.41 and 6.40. Hepatosomatic and viscerosomatic index increased as dietary CHO:L ratios increased. Low serum TG, CHL and HDL levels was observed in the groups with the CHO:L ratio of 6.40 and 20.09. Hepatic histology showed that the fish fed with dietary CHO:L ratios of 3.41 and 6.40 have more hepatic lipid droplets than fish fed with other diets. Hepatic gene expression data indicated that the cd36 transcript levels were significantly affected by dietary CHO:L ratios, with the highest expression in fish fed D4 and D5 with CHO:L ratio of 3.41 and 6.40. Higher transcript levels of lipogenic genes ( fas , acc-α and dgat2 ) were observed in the liver of fish fed with high CHO:L ratio diets, whereas FA β-oxidation genes ( cpt1 and acox1 ) expression were depressed in liver. Hepatic apoB100 expression was also down-regulated with the high dietary CHO:L ratios. These results suggested that dietary carbohydrates have a stronger effect on hepatic lipid accumulation than dietary lipids in tilapia. High dietary CHO:L ratios (> 3.41) accelerated the hepatic lipid deposition by increasing de novo lipogenesis and reducing FA β-oxidation and lipoprotein clearance in the liver, and diet with around 1.95 CHO:L ratio is suitable for tilapia culture concerning the growth performance and health. The present results will be of great significance in improving the utilization of dietary lipid and carbohydrate to achieve better nutrition efficiency in tilapia. [ABSTRACT FROM AUTHOR]

Lipid quality on shrimp nutrition is very important, especially regarding to its content of essential substances. Nutrient intake is instinctively adjusted when animal selects its feeds in the wild. In captivity, it is possible to observe the cholesterol requirement, as in shrimps, as in other crustaceans, since they do not possess the ability to synthesize this indispensable nutrient for animal survival and development. Cholesterol is a relatively small part of the essential lipids for shrimp, and cannot be replaced by phytosteroids. Since cholesterol is the precursor of the ecdisone hormone (which controls molts), it is particularly more necessary during larvae stages. Also, fat-soluble vitamins are vital for shrimp growth. However, vitamins D and K functions are still partially known, because the premises of their functions in vertebrates have no equivalent in crustaceans. In this sense, only the relevance of vitamin E to protect PUFA has an explanation so far. On the other hand, the carotenoid astaxanthin appears to be as effective as an antioxidant that is difficult to justify the need for tocopherol. Dietetic imbalance in essential lipids turns shrimps susceptible to opportunistic infections. This review describes the cholesterol and fat-soluble vitamins importance in shrimp feeding. [ABSTRACT FROM AUTHOR]

The article discusses growth and fatty acid composition and lipid deposition of Acipenser gueldenstaedtii fed different lipid sources. Topics discussed include use of vegetable oils in the aquaculture industry to completely replace fish oil, information on the requirement of lipid in Russian sturgeon, and isonitrogenous and isoenergetic semi-purified diets formulated to contain fish oil, sun- flower oil or linseed oil .

'Dulce de leche' (DL) is a rich, creamy, caramel-like milk-based sauce or spread, prepared by concentrating whole milk with sucrose, reaching a lipid content of about 6% w/w. A DL-like product with pre-emulsified pecan oil as fat source was developed. Nonfat milk, xanthan gum, sugars, pecan oil, and natural tocopherols were included in the formulation prepared in a pilot-plant scale and stored at room temperature. Pecan oil-DL showed physicochemical and rheological characteristics similar to those of commercial DL containing ∼6% milk fat. A slight increase in viscosity was observed after 2 months of storage, remaining almost constant thereafter. Crystal formation was not observed and microbial counts were low. Slight lipid oxidation was experienced after 138 days. DL fatty acid (FA) profile showed high content of unsaturated FA (UFA, 89-90% w/w), mainly oleic and linoleic acids, effectively protected by both tocopherols and antioxidant compounds. Pecan oil-DL presented good sensorial characteristics (above 7 points on a 10 point-hedonic scale), with 82.6% less saturated FA and 200% more UFA than a traditional milk fat-product, being an alternative product with lipid profile enriched in unsaturated fatty acids. Practical applications: The 'dulce de leche' (DL) jam-like developed could be considered an alternative to the traditional DL, with better nutritional value considering its lipid composition. It has a fatty acid (FA) balance in favor of unsaturated FA as well as absence of cholesterol in the formulation because it is a product elaborated with nonfat milk. DL formulated with pecan oil showed physicochemical, rheological, and sensory characteristics similar to commercial products that contain about ∼6% milk fat. It showed good stability during storage at room temperature, with no lactose crystal formation, and only slight lipid oxidation. The product was successfully developed in the laboratory and also produced in a pilot plant scale, thus, it is feasible to produce it at an industrial scale. A DL-like product with pre-emulsified pecan oil as fat source was developed. Pecan oil-DL showed physicochemical and rheological characteristics similar to those of commercial DL containing ∼6% milk fat. DL fatty acid (FA) profile showed high content of unsaturated FA (UFA, 89-90% w/w), mainly oleic and linoleic acids, effectively protected by both tocopherols and antioxidant compounds. Pecan oil-DL presented good sensorial characteristics, with 82.6% less saturated FA and 200% more UFA than a traditional milk fat-product, being an alternative product with lipid profile enriched in unsaturated fatty acids. [ABSTRACT FROM AUTHOR]

Mounting evidence in humans supports an etiological role for the microbiota in inflammatory atherosclerosis. Atherosclerosis is a progressive disease characterized by accumulation of inflammatory cells and lipids in vascular tissue. While retention of lipoprotein into the sub-endothelial vascular layer is believed to be the initiating stimulus leading to the development of atherosclerosis, activation of multiple pathways related to vascular inflammation and endothelial dysfunction sustain the process by stimulating recruitment of leukocytes and immune cells into the sub-endothelial layer. The Gram-negative oral pathogen Porphyromonas gingivalis has been associated with the development and acceleration of atherosclerosis in humans and these observations have been validated in animal models. It has been proposed that common mechanisms of immune signaling link stimulation by lipids and pathogens to vascular inflammation. Despite the common outcome of P. gingivalis and lipid feeding on atherosclerosis progression, we established that these pro-atherogenic stimuli induced distinct gene signatures in the ApoE −/− mouse model of atherosclerosis. In this study, we further defined the distinct roles of dietary lipids and P. gingivalis infection on atherosclerosis progression and the gut microbiota. We demonstrate that diet-induced lipid lowering resulted in less atherosclerotic plaque in ApoE −/− mice compared to ApoE −/− mice continuously fed a Western diet. However, the effect of diet-induced lipid lowering on plaque accumulation was blunted by P. gingivalis infection. Using principal component analysis and hierarchical clustering, we demonstrate that dietary intervention as well as P. gingivalis infection result in distinct bacterial communities in fecal and cecal samples of ApoE −/− mice as compared to ApoE −/− mice continuously fed either a Western diet or a normal chow diet. Collectively, we identified distinct microbiota changes accompanying atherosclerotic plaque, suggesting a future avenue for investigation on the impact of the gut microbiota, diet, and P. gingivalis infection on atherosclerosis. [ABSTRACT FROM AUTHOR]

Background It has been reported that lipid-rich enteral nutrition (EN) could ameliorate inflammation in various diseases. In this study, we investigated whether lipid-rich EN could control intestinal inflammation, improve intestinal motility and mucosal barrier injury after intestinal ischemia/reperfusion (I/R) injury. Methods Male adult rats received saline, conventional EN, or lipid-rich EN via gavage before and after intestinal I/R injury. The superior mesenteric artery was occluded for 60 min. The sham group underwent laparotomy without superior mesenteric artery occlusion and was administrated saline. Intestinal motility was measured 4 h after intestinal I/R injury by fluorescein isothiocyanate-dextran transit assay; the intestinal and systemic inflammation were assessed by analyzing intestinal and serum concentrations of tumor necrosis factor α, interleukin (IL)- 6, and IL-10, separately. The intestinal mucosal barrier injury was assessed by analyzing the serum levels of intestinal fatty acid–binding protein (I-FABP) and intestinal mucosal tight junction (TJ) proteins. Results The intestinal I/R injury decreased intestinal motility and intestinal mucosal TJs expression significantly when compared with the sham group ( P < 0.05). The intestinal and systemic inflammatory parameters and the serum I-FABP were also significantly higher in the I/R groups than those in the sham group ( P < 0.05). Both conventional and lipid-rich EN increased the intestinal motility and the intestinal mucosal TJs expression and decreased the intestinal and systemic inflammatory parameter and serum I-FABP levels to different degrees when compared with the I/R group ( P < 0.05). However, lipid-rich EN significantly improved the negative alterations in these biochemical parameters when compared with the conventional EN ( P < 0.05). Conclusions These results suggest that lipid-rich EN might be able to control intestinal inflammation, improve intestinal motility and mucosal barrier injury after intestinal I/R injury. Thus, the administration of lipid-rich EN may be an effective treatment for promoting gastrointestinal function recovery after intestinal I/R injury. [ABSTRACT FROM AUTHOR]

This study examined the fatty acid and mineral compositions of raw and cooked beef and liver from bonsmara (BD) and non-descript (ND) cattle, raised on natural pasture. Samples were collected from 80 cows and thermal-processed at 65°c for 120 minutes and 85°c for 60 minutes using sous-vide techniques, and then analysed for fatty acid and mineral compositions. The results did not show differences in individual fatty acid composition of meat samples between the breed (p>0.05). However, the raw beef sample had higher content of monounsaturated fatty acids (MUFA, 40.22% ± 3.79 ND, 42.53% ± 2.39 BD) and lower content of polyunsaturated fatty acids (PUFA, 11.02% ± 5.47 ND, 10.13% ± 6.73 BD) than liver (MUFA, 20.11% ± 4.76 ND, 21.08% ± 2.46 BD; PUFA, 30.73% ± 5.20 ND, 31.11% ± 2.37 BD) (p<0.05). The PUFA/MUFA and n-6/n-3 ratios, atherogenicity and desaturase indices were comparable between breeds, but higher in beef than liver. The total percentage of saturated fatty acid, MUFA and PUFA retained after cooking were numerically higher in liver than in beef (p>0.05). The results further revealed higher contents of Na, Mg, and Zn in raw beef than liver. However, the content of Na, Mg, and Zn in the beef and liver were not significantly (P>0.05) affected after cooking. Based on the PUFA proportion of the total lipids in beef (11.02%) and liver (31.11%) and n-6/n-3 PUFA ratio in beef (2.54) and liver (1.34), consumption of liver and beef from cattle raised on pasture could be beneficial to human health. Additionally, application of sous-vide technique can be used to minimize the nutritional losses in beef and liver. [ABSTRACT FROM AUTHOR]

Objective: The current study aimed to investigate the impact of high-fat diets composed of different animal and vegetable fat sources on serum metabolic health markers in Japanese quail, as well as the overall lipid content and fatty acid profiles of the edible bird tissues following significantly increased dietary lipid supplementation. Methods: Fifty seven male quail were divided into six groups and fed either a standard diet or a diet enriched with one of five different fats (22% coconut oil, lard, palm oil, soybean oil, or sunflower oil) for 12 weeks. The birds were subjected to an oral glucose tolerance test following the feeding period, after which they were euthanized and blood, liver, breast, and thigh muscle samples collected. Total fat content and fatty acid profiles of the tissue samples, as well as serum uric acid, triglyceride, cholesterol, total protein, albumin, aspartate transaminase, and total bilirubin concentrations were assessed. Results: High-fat diet feeding had no significant effects on the glucose tolerance of the birds. Dietary fatty acid profiles of the added fats were reflected in the lipid profiles of both the liver and breast and thigh muscle tissues, indicating successful transfer of dietary fatty acids to the edible bird tissues. The significantly increased level of lipid inclusion in the diets of the quail used in the present study was unsuccessful in increasing the overall lipid content of the edible bird tissues. Serum metabolic health markers in birds on the high-fat diets were not significantly different from those observed in birds on the standard diet. Conclusion: Thus, despite the various high-fat diets modifying the fatty acid profile of the birds' tissues, unlike in most mammals, the birds maintained a normal health status following consumption of the various high-fat diets. [ABSTRACT FROM AUTHOR]

Rabbitfish Siganus canaliculatus is the first marine teleost reported to have the ability of biosynthesizing C 20–22 long-chain polyunsaturated fatty acids (LC-PUFA) from C 18 precursors, and thus provides a model for studying the regulatory mechanisms of LC-PUFA biosynthesis in teleosts. To investigate the possible roles of peroxisome proliferator-activated receptors (PPARs), critical transcription factors involved in the regulation of lipid metabolism, in the regulation of LC-PUFA biosynthesis in rabbitfish, the PPAR genes were cloned and their expression characterization with PPAR agonists, dietary lipid resource, and ambient salinity were examined. Three cDNA sequences respectively encoding 477, 516 and 519 amino acids of PPARα, PPARβ, and PPARγ isoforms were obtained. PPARα exhibited a wide tissue expression with its highest levels in the heart and brain; PPARβ was predominantly expressed in the gills, while PPARγ was highly expressed in the intestine and gills. In rabbitfish primary hepatocytes, both the PPAR agonists 2-bromopalmitate (2-Bro) and fenofibrate (FF) increased the expression of PPARγ, SREBP1c and Elovl5, whereas FF depressed the expression of Δ6/Δ5 Fad. Moreover, a higher hepatic PPARβ expression was observed in fish fed diets with vegetable oils (VO) than that with fish oil (FO), in the former the expression of PPARα, PPARβ, and PPARγ were increased at the low ambient salinity (10 ppt), where an increasing expression of Δ5/Δ6 Fad, Δ4 Fad and Elovl5 genes was previously reported. These results suggest that PPARs might be involved in the upregulation of LC-PUFA biosynthesis with dietary VO and low ambient salinity in rabbitfish. [ABSTRACT FROM AUTHOR]