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The study aimed to investigate the effect of Huoxiang longhuang powder on the serum biochemical, antioxidant and immune indexes of fattening cattle. Eighty healthy, disease-free, well-conditioned Simmental crossbred cattle with an average weight of (385.0 ± 16.3) kg and similar age were selected and randomly divided into four groups, with four replicates in each group and five cattle in each replicate. The cattle in the control group, experimental group I, experimental group H, and experimental group M were fed with rations containing 0, 1%, 2%, and 4% Huoxiang longhuang powder, respectively. The preliminary trial lasted for 10 days, followed by a formal experimental period of 60 days. The results showed that compared to the control group, the total protein, globulin content, and alkaline phosphatase activity of fattening cattle in the experimental group H increased by 10.87%, 17.83% and 24.82%, respectively (P<0.05), and the urea nitrogen content decreased by 20.25% (P<0.05). Compared to the control group, the superoxide dismutase, catalase activities, and total antioxidant capacity in the serum of fattening cattle in the experimental group H increased by 36.70%, 28.63% and 35.28%, respectively (P<0.05). The concentrations of immunoglobulin A and immunoglobulin G of fattening cattle in the experimental group H increased by 18.99% and 29.98%, respectively (P<0.05). The study indicates that adding 2% Huoxiang longhuang powder to the diet of fattening cattle can improve the serum biochemical indexes, promote the deposition of fat and protein, and enhance antioxidant and immunity capabilities. [ABSTRACT FROM AUTHOR]

Objective: The purpose of this study was to analyze the mechanism by which STAT5B inhibits ferroptosis in mantle cell lymphoma (MCL) by promoting DCAF13 transcriptional regulation of p53/xCT pathway., Methods: The correlations between STAT5B, DCAF13 and ferroptosis in MCL were analyzed using Gene Expression Profiling Interactive Analysis (GEPIA, http://gepia.cancer-pku.cn/index.html). The expression levels and pairwise correlations of STAT5B, DCAF13, p53 and xCT in MCL patients were detected, respectively. STAT5B was silenced to confirm their criticality in MCL ferroptosis. the effects of blocking necrosis, apoptosis and ferroptosis on the anti-MCL effects of STAT5B were examined. Cells with STAT5B overexpression and/or DCAF13 silencing were constructed to confirm the involvement of DCAF13 in the STAT5B-regulated p53/xCT pathway. The regulation of p53 ubiquitination was confirmed by DCAF13 overexpression and MG132. The effects of silencing DCAF13 and MG132 on STAT5B overexpression on MCL was clarified by a tumor-bearing nude mouse model., Results: DCAF13 was overexpressed in MCL and positively correlated with STAT5B, negatively correlated with p53, and positively correlated with xCT. Inhibition of ferroptosis alleviated the inhibitory effects of siSTAT5B on MCL, while inhibition of necrosis and apoptosis had few effects. Silencing of DCAF13 led to the blocking of STAT5B regulation of p53/xCT and ferroptosis. The changes in DCAF13 and the addition of MG132 did not have statistically significant effects on p53 mRNA. Elevation of DCAF13 resulted in downregulation of p53 protein levels, and this inhibition was reversed by MG132. In animal models, the promotion of MCL and the inhibition of ferroptosis by STAT5B. Silencing of DCAF13 blocked STAT5B inhibition of p53 and induction of xCT, GPX4, and GSH., Conclusion: STAT5B suppresses ferroptosis by promoting DCAF13 transcription to regulate p53/xCT pathway to promote MCL progression., Competing Interests: The authors report no conflicts of interest in this work., (© 2024 Zhang et al.)