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The successful diagnosis of dermatomycosis depends on specimen collection. Dermatomycosis is sampled mainly for scales, but there is a lack of research on specimens of blister fluid.To explore whether blister fluid can diagnose dermatomycosis and compare blister fluid and scale specimens for dermatomycosis diagnosis.From April to July 2021, we prospectively gathered 34 patients who needed to meet all inclusion criteria simultaneously and collected their blister fluid and scales as specimens. The two samples were tested by fluorescent stain microscopy, fungal culture and PCR, and the diagnosis results were compared.The blister fluid sample's sensitivity, specificity and accuracy were 90%, 100% and 94.1%, respectively, whereas the scales sample were 60%, 100% and 76.5%, respectively. The positive likelihood ratios were10 for both blister fluid and scales specimen, and the negative likelihood ratios were not0.1. On the Youden's index, the blister fluid specimen was 90%, and the scales specimen was 60%. As for the diagnostic odds ratio, both of them were1. By fungal culture, we detected 14 cases of fungi in blister fluid and eight in scales. On PCR, 22 cases of fungi in blister fluid and ten in scales were identified.This study demonstrated that a sample of blister fluid had better sensitivity, accuracy and Youden's index in diagnosing dermatomycosis with blister fluid. Collection of blister fluid might compensate for the inadequacy of collecting only scales specimens for mycological testing.

Polyploid plants often show improved resistance against many diseases, but whether they show increased resistance to grey mould, a devastating disease caused by Botrytis spp. fungi, is seldom reported. Stomata and reactive oxygen species (ROS) play dual roles in defence against grey mould, and it is unclear how their roles change after polyploidization. We addressed these questions in diploid and colchicine-induced Lilium rosthornii after B. elliptica infection. Tetraploids were less susceptible to grey mould, with lower morality rates in naturally infected plants. Before the stomata closed in artificially infected leaves, tetraploids, with larger stomatal apertures, were more easily invaded by the pathogen than diploids. However, the lesion area increased more slowly in tetraploids than in diploids, which may be explained by three causes based on histological and physiological characteristics. First, the pathogen required more time to penetrate the epidermis and closed stomata in tetraploids than in diploids. Second, the pathogen penetrated the reopened stomata more easily than the epidermis, and stomatal density was lower in tetraploids than in diploids. Third, tetraploids showed faster ROS accumulation, a more effective ROS-scavenging system and less malondialdehyde (MDA) accumulation than diploids. Stomatal starch and abnormal guard cell nuclei were present in the infected leaves. This phenomenon may be caused by oxalic acid, a pathogenic factor for many pathogens that promotes stomatal starch degradation and stomatal reopening in Sclerotinia spp., a pathogen closely related to Botrytis spp. This suggestion was primarily confirmed by immersing healthy leaves in oxalic acid solution.

Gray mold disease, mainly caused by Botrytis elliptica (Berk.) Cooke and Botrytis cinerea Pers., leads to severe losses in lily cut flower and bulb production. MYC2 is a critical regulator of the activation of jasmonate-mediated defense responses in plants; however, information about the lily MYC2 gene is limited. Therefore, functional characterization of MYC2 in lily, especially its role in plant immune responses, should be performed. Here, significant differences between the Botrytis-resistant Lilium hybrid ‘Sorbonne’ and the Botrytis-susceptible ‘Tresor’ were found following B. cinerea inoculation, as indicated by jasmonic acid (JA) and JA-isoleucine (JA-Ile) accumulation and related gene expression. More JA and JA-Ile were detected in ‘Sorbonne’ than in ‘Tresor’ following fungal inoculation, and higher transcript levels of JA biosynthesis genes (LhAOS, LhAOC, and LhOPR3) and a signaling gene (LhCOI1) were detected in ‘Sorbonne’ than in ‘Tresor’. In contrast, expression of the critical signaling regulator LhMYC2 was higher in ‘Tresor’ than in ‘Sorbonne’. LhMYC2 was then isolated from ‘Sorbonne’ and found to be similar to several plant MYC2 homologs that have pivotal roles in JA signaling. The expression of LhMYC2 increased significantly in response to JA and salicylic acid (SA) in ‘Sorbonne’. Ectopic expression of LhMYC2 in Arabidopsis resulted in greater susceptibility to B. cinerea than that observed in wild-type plants. This susceptibility was coupled with the transcriptional changes in SA- and JA-responsive genes. Overall, our findings indicate that LhMYC2 plays a negative role in Arabidopsis resistance to B. cinerea.

Background In China, the prevalence of superficial fungal infections of the foot is high and recurrence is common. However, a prospective, large-scale and multicentre study on the aetiology of superficial fungal infections of the foot is still lacking. Objectives To study the epidemiology of aetiological agents of superficial fungal infections of the foot in urban outpatients in mainland China, as well as to understand the aetiology features of the pathogenic agent. Methods The study was designed as a multicentre, prospective epidemiological survey. A total of 1704 subjects were enrolled from seven geographical areas in mainland China. For each subject, one mycological sample and one bacterial sample were collected. KOH wet mount examination and culture were performed at local laboratories. The bacterial results were only reported in those with positive mycology. Further morphological identification and, if necessary, molecular biological identification were conducted in a central laboratory. Results Of 1704 enrolled subjects, 1327 (77.9%) subjects had positive fungal culture results. The incidence of dermatophytes, yeasts and moulds was 90.1%, 8.1% and 1.1%, respectively. The most frequently isolated aetiological agent (fungus) was Trichophyton rubrum. Moccasin form was the most commonly reported clinical diagnosis of superficial fungal infections. The most frequently isolated bacterial genus in patients was Staphylococcus. Conclusion This study prospectively investigated the clinical and mycological features of human dermatophytosis in mainland China. T rubrum was the most frequently isolated fungus, and moccasin form was the most commonly reported clinical diagnosis of superficial fungal infections.

Polyploids generally show strong environmental adaptation and diverse morphological variations. Therefore, polyploid induction is an important protocol for plant breeding. Lilium rosthornii is a wild lily species with high horticultural value and excellent disease resistance. In this study, seeds of L. rosthornii were subjected to polyploidy induction treatments to obtain multiple genotypes of tetraploids. Germinated seeds were immersed in two antimitotic agents at different concentrations for various times. In total, 199 tetraploid genotypes were obtained. The most efficient treatments of each agent were immersed in 0.05% colchicine for 36 h and in 0.01% oryzalin for 24 h; the induction rate of the former (27.78%) was significantly higher than that of the latter (22.22%). The swollen hypocotyl phenotype after colchicine and oryzalin treatments was strongly correlated with tetraploidy (0.989** and 0.975**, respectively), suggesting that this phenotype could serve as an early ploidy selection trait. The correlations were weaker between stomata length/density and tetraploidy (0.773** and 0.695**, respectively), implying that stomatal characters are affected by both the ploidy level and genotype. After several rounds subculture in vitro, the morphology and growth traits were not significantly different between diploids and tetraploids, but there were wider variations in these parameters in tetraploids than in diploids. After transplanting, the bulblet germination rate was higher in tetraploids than in diploids. The leaf phenotype did not differ between tetraploids and diploids initially, but the leaves of tetraploids became larger than those of diploids over time. Together, these suggest that tetraploids, may contribute diverse characteristics to lily breeding. Multiple genotypes of tetraploid Lilium rosthornii were induced by colchicine and oryzalin. Swollen hypocotyl was associated with polyploidy. Compared with diploids, tetraploids showed wider variations in morphological parameters.

Emergence agitation (EA) is a common complication in pediatric patients after general anesthesia. The effectiveness of magnesium sulfate in decreasing the incidence of EA in children remains controversial. Therefore, a systematic review and meta-analysis was performed to assess the efficacy of magnesium sulfate in preventing EA in pediatric patients following general anesthesia.Systematic review and meta-analysis.PubMed, Embase, Web of Science, and Cochrane Library were searched to identify eligible randomized controlled trials from their respective database inception dates to June 30, 2021.Pediatric patients (18 years old) undergoing general anesthesia.Intravenous administration of magnesium sulfate.The primary outcome of the meta-analysis was EA incidence. The risk of bias of the included studies was evaluated using the revised Cochrane risk of bias tool for randomized trials (RoB 2.0). Grading of Recommendations, Assessment, Development, and Evaluation was applied to assess the level of certainty.Eight studies with 635 participants were identified. The forest plot revealed no significant difference in the incidence of EA between patients treated with magnesium sulfate and the control group (risk ratio = 0.69, 95% confidence interval [0.44, 1.07]; P = 0.10, IAdministration of magnesium sulfate during general anesthesia did not affect the occurrence of EA in pediatric patients. However, magnesium sulfate can prolong the emergence time without adverse effects. PROSPERO registration number: CRD42021252924.

Crossing between Lilium OT (Oriental × Trumpet) cultivars and L. regale could provide an accessible way to develop lily cultivars with both horticultural traits and stress resistance. Physical mapping of 45S ribosomal DNA (rDNA) loci by fluorescence in situ hybridization (FISH) can help understand genetic relationships and chromosome inheritance in lily breeding. In this study, hybrid progeny were obtained from the combinations of ‘Conca d’Or’ × L. regale and ‘Saltarello’ × L. regale, confirming the fertility of OT cultivars as female parents in crosses with L. regale. ‘Saltarello’ × L. regale had the highest embryo rate (8.96), indicating cross-compatibility between ‘Saltarello’ and L. regale. Based on the results of crossing and FISH, the genetic relationship between OT cultivars and L. regale was further investigated. ‘Saltarello’ possessed twelve 45S rDNA signal loci, of which eight were similar to those of L. regale, on chromosomes 1, 2 and 5, confirming the closest genetic relationship with L. regale, ‘Conca d’Or’ with five similar sigals second and ‘Manissa’ with three similar sigals farthest. The 45S rDNA loci in hybrids of ‘Saltarello’ ×L. regale were located on chromosomes 1 through 6 and varied in number from 7 to 13. These hybrids included both euploids and aneuploids, demonstrating that allotriploid Lilium OT hybrids can produce viable gametes with euploid and aneuploid chromosome numbers. Thus, it should be possible to select new cultivars from interspecific hybrids of OT hybrids × L. regale.

Lilium is an important commercial flowering species, and there are many varieties and more than 100 species of wild Lilium. Lilium × formolongi is usually propagated from seedlings, and the flowering of these plants is driven mainly by the photoperiodic pathway. Most of the other lily plants are propagated via bulblets and need to be vernalized; these plants can be simply divided into pretransplantation types and posttransplantation types according to the time at which the floral transition occurs. We identified three Lilium FLOWERING LOCUS T (LFT) family members in 7 Lilium varieties, and for each gene, the coding sequence of the different varieties was identical. Among these genes, the LFT1 gene of Lilium was most homologous to the AtFT gene, which promotes flowering in Arabidopsis. We analyzed the expression patterns of LFT genes in Lilium × formolongi seedlings and in different Lilium varieties, and the results showed that LFT1 and LFT3 may promote floral induction. Compared with LFT3, LFT1 may have a greater effect on floral induction in Lilium, which is photoperiod sensitive, while LFT3 may play a more important role in the floral transition of lily plants, which have a high requirement for vernalization. LFT2 may be involved in the differentiation of bulblets, which was verified by tissue culture experiments, and LFT1 may have other functions involved in promoting bulblet growth. The functions of LFT genes were verified by the use of transgenic Arabidopsis thaliana plants, which showed that both the LFT1 and LFT3 genes can promote early flowering in Arabidopsis. Compared with LFT3, LFT1 promoted flowering more obviously, and thus, this gene could be an important promoter of floral induction in Lilium.

The live broadcast of the common league mainly adopts the design of streaming media data block by block. The secondary processing of streaming data is carried out on the server-side. It results in a higher delay during a live broadcast. The higher delay has numerous disadvantages during broadcasting for delay-sensitive applications. We have to solve the delay problems during a live broadcast. Therefore, in this paper, we optimize the synchronization of the tennis professional league live broadcast based on wireless network planning. In the proposed scheme, the 3D detection target model is first constructed, and the background is extracted from the moving video image. The extraction is performed using the interframe difference elimination algorithm, and the target motion trajectory is predicted. Then the phase difference characteristics of the image foreground trajectory are analyzed by the Hilbert transform to detect target missing points. By observing the vertex structure of the target missing point, the phase Fourier transform frame is constructed. Finally, the synchronization of the league live broadcast is encoded and decoded. Based on the timestamp, the synchronization optimization of the tennis professional league live broadcast is completed. The experimental results show that the synchronization optimization method has good synchronization, high resolution, short reaction time, and good detection effect.

Background: The selection of reliable reference genes is a critical element for obtaining accurate gene expression data to assess quantitative real-time polymerase chain reaction (qRT-PCR) performance. It is critical to use suitable reference genes in miRNA qRT-PCR because of short amplification products and large differences in the expression levels of target miRNAs involved in some biological processes. However, in lily, which exhibits a large complex genome but lacks a reference, the available miRNA reference genes for use in qRT-PCR under various treatment conditions are limited, and their reliability has rarely been systematically evaluated.Results: In this study, 8 candidate reference genes, including three classic housekeeping genes and five potential miRNAs from the miRNA library of L. × formolongi, were selected and assessed for expression stability utilizing the BestKeeper, geNorm and Normfinder tools, together with the Delta Ct method, across a diverse set of biotic and abiotic experimental conditions (developmental stages, tissues, heat stress and pathogen defence) to determine the best reference gene(s) for L. × formolongi and L. regale. The final ranking was reordered by using RankAggreg, and the results showed that the novel miRNA PC-3p-67_108977 and the conserved miRNAs miR399a, miR399a and U6 were the most stable genes for L. × formolongi and L. regale, respectively, under all tested experimental conditions. Additionally, PC-3p-67_108977 and U6 were the most suitable genes for qRT-PCR studies in lily.Conclusions: This study provides a comprehensive evaluation of the reliability of reference genes for miRNA studies on development and biotic and abiotic stress responses in different lilies. These results will be beneficial for miRNA identification and functional studies of lilies in the future.