1. Oligoclonal Band Straightening Based on Optimized Hierarchical Warping for Multiple Sclerosis Diagnosis
- Author
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Farah Haddad, Samuel Boudet, Laurent Peyrodie, Nicolas Vandenbroucke, Julien Poupart, Patrick Hautecoeur, Vincent Chieux, Gérard Forzy, Laboratoire de physique des interfaces et des couches minces [Palaiseau] (LPICM), École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS), Unité de traitement des signaux Biomédicaux (UTSB), Université Catholique de Lille - Faculté de Médecine, Maïeutique, Sciences de la santé (FMMS), Institut Catholique de Lille (ICL), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Institut Catholique de Lille (ICL), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), LAGIS-SI, Laboratoire d'Automatique, Génie Informatique et Signal (LAGIS), Université de Lille, Sciences et Technologies-Centrale Lille-Centre National de la Recherche Scientifique (CNRS)-Université de Lille, Sciences et Technologies-Centrale Lille-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Informatique Signal et Image de la Côte d'Opale (LISIC), Université du Littoral Côte d'Opale (ULCO), Département de neurologie [Lille], and Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)
- Subjects
Multiple Sclerosis ,Chemical technology ,Oligoclonal Bands ,band straightening ,tears ,Reproducibility of Results ,TP1-1185 ,Biochemistry ,Atomic and Molecular Physics, and Optics ,cerebrospinal fluid ,[INFO.INFO-AI]Computer Science [cs]/Artificial Intelligence [cs.AI] ,Analytical Chemistry ,gel electrophoresis ,[INFO.INFO-AU]Computer Science [cs]/Automatic Control Engineering ,Immunoglobulin G ,Humans ,Electrical and Electronic Engineering ,Isoelectric Focusing ,image warpin ,[SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing ,Instrumentation ,multiple sclerosis ,isoelectric focusing ,oligoclonal bands ,immunoglobulin G ,image warping - Abstract
International audience; The detection of immunoglobulin G (IgG) oligoclonal bands (OCB) in cerebrospinal fluid (CSF) by isoelectric focusing (IEF) is a valuable tool for the diagnosis of multiple sclerosis. Over the last decade, the results of our clinical research have suggested that tears are a non-invasive alternative to CSF. However, since tear samples have a lower IgG concentration than CSF, a sensitive OCB detection is therefore required. We are developing the first automatic tool for IEF analysis, with a view to speeding up the current visual inspection method, removing user variability, reducing misinterpretation, and facilitating OCB quantification and follow-up studies. The removal of band distortion is a key image enhancement step in increasing the reliability of automatic OCB detection. Here, we describe a novel, fully automatic band-straightening algorithm. The algorithm is based on a correlation directional warping function, estimated using an energy minimization procedure. The approach was optimized via an innovative coupling of a hierarchy of image resolutions to a hierarchy of transformation, in which band misalignment is corrected at successively finer scales. The algorithm’s performance was assessed in terms of the bands’ standard deviation before and after straightening, using a synthetic dataset and a set of 200 lanes of CSF, tear, serum and control samples on which experts had manually delineated the bands. The number of distorted bands was divided by almost 16 for the synthetic lanes and by 7 for the test dataset of real lanes. This method can be applied effectively to different sample types. It can realign minimal contrast bands and is robust for non-uniform deformations.
- Published
- 2021