1. Gene silencing of stearoyl-ACP desaturase enhances the stearic acid content in Chlamydomonas reinhardtii
- Author
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L. de Jaeger, Emil J.H. Wolbert, Dirk E. Martens, René H. Wijffels, Jan Springer, and Gerrit Eggink
- Subjects
Fatty Acid Desaturases ,0106 biological sciences ,0301 basic medicine ,Bio Process Engineering ,Environmental Engineering ,Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 [VDP] ,Chlamydomonas reinhardtii ,Bioengineering ,behavioral disciplines and activities ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,mental disorders ,microRNA ,Gene silencing ,Gene Silencing ,Waste Management and Disposal ,VLAG ,chemistry.chemical_classification ,Messenger RNA ,Enhanced stearic acid ,biology ,Renewable Energy, Sustainability and the Environment ,food and beverages ,General Medicine ,biology.organism_classification ,RNA silencing ,Oleic acid ,BBP Bioconversion ,030104 developmental biology ,Enzyme ,chemistry ,Biochemistry ,Matematikk og Naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474 [VDP] ,lipids (amino acids, peptides, and proteins) ,Stearic acid ,Stearic Acids ,psychological phenomena and processes ,Oleic Acid ,010606 plant biology & botany - Abstract
In this study, stearoyl-ACP desaturase (SAD), the enzyme that converts stearic acid into oleic acid, is silenced by artificial microRNA in the green microalga Chlamydomonas reinhardtii. Two different constructs, which target different positions on the mRNA of stearoyl-ACP desaturase, were tested. The mRNA levels for SAD were reduced after the silencing construct was induced. In one of the strains, the reduction in SAD mRNA resulted in a doubling of the stearic acid content in triacylglycerol molecules, which shows that stearic acid production in microalgae is possible.
- Published
- 2017