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1. National quality control and validation of hepatitis C NS3, NS5A and NS5B genotypic resistance testing

Author

M.P. Callegaro, A. Lai, Enzo Boeri, G. Raimondo, E. Galmozzi, Francesca Ceccherini Silberstein, N. Cuomo, M.A.D. Stefano, Valeria Micheli, Antonio Craxì, C.F. Perno, M. Arosio, Fausto Baldanti, C. Minosse, Stefania Paolucci, Maurizio Zazzi, Maria Rosaria Capobianchi, Rosaria Santangelo, Coppola Nicola, M. L. Vatteroni, Maurizio Sanguinetti, Marianna Aragri, Valeria Cento, Giuseppina Raffa, Teresa Pollicino, Maurizia Rossana Brunetto, C. Caudai, S. Soldini, Silvia Galli, Stefano Menzo, T. Ruggiero, Valeria Ghisetti, William Gennari, D. Cavallone, Anna Rosa Garbuglia, Bianca Bruzzone, T. Santantonio, A. Raddi, Mario Starace, and Laura Monno

Subjects
NS3, Hepatology, business.industry, media_common.quotation_subject, Hepatitis C, Biology, medicine.disease, Biotechnology, chemistry.chemical_compound, chemistry, medicine, Genotypic resistance, Quality (business), business, NS5A, NS5B, media_common
Published
2018
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2. National quality control and validation of hepatitis C NS3, NS5A and NS5B genotypic resistance testing

Author

Valeria Cento, Maurizio Zazzi, Teresa Pollicino, Anna Rosa Garbuglia, S. Soldini, M. Arosio, Silvia Galli, A. Raddi, Annapaola Callegaro, Enzo Boeri, Marianna Aragri, Valeria Ghisetti, C. Minosse, N. Cuomo, Rosaria Santangelo, Daniela Cavallone, M. L. Vatteroni, Maurizia Rossana Brunetto, Nicola Coppola, Giuseppina Raffa, T. Santantonio, A. Lai, G. Raimondo, M. Di Stefano, C.F. Perno, Stefania Paolucci, Antonio Craxì, Valeria Micheli, William Gennari, T. Ruggiero, F. Ceccherini-Silberstein, E. Galmozzi, Mario Starace, Michela Sampaolo, Bianca Bruzzone, Laura Monno, Maria Rosaria Capobianchi, Maurizio Sanguinetti, C. Caudai, Stefano Menzo, and Fausto Baldanti

Subjects
0301 basic medicine, NS3, Hepatology, business.industry, media_common.quotation_subject, Gastroenterology, Hepatitis C, medicine.disease, Biotechnology, 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, chemistry, medicine, Genotypic resistance, Quality (business), business, NS5A, NS5B, media_common
Published
2018
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3. Contents, Vol. 77, 1997

Author

A. Botta, K. Rojas, P. Zaragoza, X.-X. Zhang, M. Pritchard, B.P. Chowdhary, J.P. Park, J.L. Kissil, A. Rocchi, T.K. Watanabe, T. Siddque, N.P. Mertvetsov, W. Engel, A.A. Bosma, C. Rodellar, A. Arai, F. Shimizu, B. Hoebee, K.N. Sastry, R. Houlgatte, P.A. Voûte, N. Guo, U.W. Kenkare, J.-J. Cassiman, J. Guimera, W.R. Harrison, M.Z. Limongi, G. Mirza, A. Fratello, C.H. van Os, T. Ikeuchi, M. Chaffanet, T. Goldammer, M. Mannens, D. Grady, D. Wells, V. Romano, O. Miura, As. Ricco, M. Schwerin, M. Gersh, M.L. Filipenko, S.A. Wilcox, H. Levéziel, S.J. O’Brien, G.F.M. Merkx, C.C. Morton, G. Hardiman, R. Marzella, S. Hirosawa, T.J. Robinson, K.B.M. Reid, C. Elduque, A.S. Hewson, P.M.T. Deen, J.A. Squire, J.A.M. Graves, L. Vatteroni, E. Viegas-Péquignot, K. Yamamoto, M. Carter, L. Frönicke, N.V. Vorobieva, J. Overhauser, N. Ceratto, N.A. de Haan, M. Suzuki, T. Kozaki, M.S. Aly, B. Redeker, S. van Beersum, P.M. Borodin, F.F.B. Elder, A. Kimchi, A.S. Graphodatsky, B. Beatty, J.A. McMahon, M. de Meulemeester, J.B. Searle, I.V. Koroleva, W.Y. Hung, Y. Kuga, M. Jeanpierre, N. Sakuragawa, S. Feo, C. Auffray, M. Ogawa, M. Rocchi, M.P. Hande, C.K. Ullrich, J. Widmer, A. Ponce de León, F.O. Fackelmayer, S. Weremowicz, A. Pizzuti, K. Ohsugi, S. Okuno, R.J.M. Bindels, L.D. Matyakhina, A.P. McMahon, R. Leube, Y. Yang, A. Pandita, M. Lachtermacher, A. Tanzariello, B. Dallapiccola, M.A. Ferguson-Smith, A. Musio, A.F. Davies, D. Patterson, N. Lynch, Y. Nakamura, G. Rainaldi, M. Steenman, S.-T. Lee, H. Hayes, P.C.M. O’Brien, G.G. Karpova, C.H.M. Mellink, E. Jenkins, J.H. Xia, M. Schepens, A.T. Natarajan, B.-L. Lim, R. Meneveri, W.G. Nash, J. Kissing, M. Stacey, T. Fujiwara, M. Schmid, L.A. Witters, C. Zijlstra, L. Viggiano, F. Yang, M. Nagata, W. Bie, H. Scherthan, H. Murer, B. Ghebrehiwet, A. Westerveld, S. Kurata, H.N. Seuánez, M. Lovett, K.-H. Lee, R. Godbout, H.G. Brunner, J.M. Varley, P. Thygesen, R. Slater, S. Ishikawa, S.D. Pack, E. Takahashi, O.V. Cheryaukene, C. Bendixen, F. Ugolini, M. Matsumoto, R.M. Brunner, A. Risch, D. Birnbaum, Y. Takei, C.H. Fan, L.A. James, E.M. Ladenburger, P. Laurent, G. von Beust, T.K. Mohandas, H. Kobayashi, E. Burt, K. Wiesmeijer, M. Kai, A. Baldini, P. Eydoux, F.C. Canavez, F. Pelliccia, A. Geurts van Kessel, Déborah Bourc'his, S.-H. Park, S. Sebastian, C. Dobkin, R. Stanyon, R.A. Kastelein, L. Langbein, R. Toder, K. Okui, O.L. Serov, N. Matas, M.R. Koehler, R. Moyzis, J.F. Bazan, M.A. van Kuijck, A. Simons, P. Miniou, Y. Yokoyama, D. Molina Gomes, L. Xu, M.A.M. Moreira, H. Kim, E. Sim, J. Ragoussis, F. Saito-Ohara, M. Kool, N. Miyasaka, T. Katagiri, P. Bosco, X. Estivill, N. Archidiacono, G. Novelli, R. Knippers, P. Maccarone, J. Wienberg, M.-J. Pébusque, H.S. Tenenhouse, M. Nadal, W. Schwaeble, H. Hameister, H. van Bokhoven, T. Takahashi, E.I.B. Peerschke, V. Jurecic, X.-L. Yao, Y. Hey, P. Riegman, S.N. Malchenko, H.X. Deng, A.B. Spurdle, and N. Hoggard

Subjects
Botany, Genetics, Zoology, Biology, Molecular Biology, Genetics (clinical)
Published
1997
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4. Accumulation of anchorage independent cells showing amplified genes (CAD) during the in vitro propagation of CHEF18 Chinese hamster cells

Author

A. Piras, L. Vatteroni, G. Rainaldi, T. Mariani, and Maria A. Caligo

Subjects
Phosphonoacetic Acid, Drug Resistance, Mice, Nude, Hamster, Chinese hamster, Cell Line, Mice, Cricetinae, Gene duplication, Aspartate Carbamoyltransferase, Cell Adhesion, Animals, Thioguanine, Gene, Dihydroorotase, Genetics, Aspartic Acid, Mice, Inbred BALB C, biology, Gene Amplification, Neoplasms, Experimental, Cell Biology, General Medicine, biology.organism_classification, Phenotype, In vitro, Cell biology, Transformation (genetics), Cell Transformation, Neoplastic, Cell culture, Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)
Abstract

Anchorage independence and gene amplification have frequently been associated with a transformed or tumorigenic phenotype in cultured mammalian cells. However, it is unknown whether these two traits occur as related events during transformation, or are independent features of the transformed phenotype. To clarify this point, immortalized, untransformed CHEF18 Chinese hamster cells were propagated in culture until they became transformed and tumorigenic. The frequencies with which CHEF18 cells formed colonies either in soft agar, in medium containing N-phosphonacetyl-L-asparate or in the two selective media simultaneously, were determined. The results indicate that anchorage independence and CAD gene amplification spontaneously arose during the propagation of the cells and that their concurrent emergence was not the consequence of independent events. However, the kinetics of their appearance suggests that anchorage independence is the early event whereas gene amplification might represent one of the numerous events which can be dynamically selected in anchorage-independent cells.

Published
1993
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5. Reduction of proliferative heterogeneity of CHEF18 Chinese hamster cell line during the progression toward tumorigenicity

Author

L. Vatteroni, L. Citti, Antonio Piras, S. Simi, B. Pinto, and G. Rainaldi

Subjects
Time Factors, Clinical Biochemistry, G1 Phase, Fluorescent Antibody Technique, Genetic Variation, Hamster, Cell Biology, General Medicine, Fibroblasts, Cell cycle, Biology, biology.organism_classification, Chinese hamster, Cell biology, Cell Transformation, Neoplastic, Cricetulus, Cell culture, Cricetinae, Coming out, Animals, Doubling time, Stem cell, Developmental biology, Cell Division, Developmental Biology
Abstract

Doubling time and generation time represent two parameters by which the proliferation of cultured mammalian cells can be monitored. In this study we report the characterization of CHEF18 Chinese hamster cell line during the progression toward tumorigenicity by analysis of doubling time and generation time. The two parameters reveal that the proliferation was initially different, indicating the presence of a proliferative heterogeneity among the cycling cells. The progressive reduction up to the disappearance of this discrepancy suggests that a modification of the length of some phases of the cell cycle may have occurred during the progression toward tumorigenicity. However, the hypothesis that the shortening of doubling time might be due to a continuous coming out of cells from the cell cycle rather than to a shortening of the cell cycle is presented.

Published
1991
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6. Torquetenovirus in patients with arthritis

Author

J. Rocchi, Steven Specter, Silvia Meschi, M. L. Vatteroni, F. Maggi, Mauro Bendinelli, L. Ceccherini-Nelli, E. Andreoli, A. Delle Sedie, and L. Riente

Subjects
Adult, Male, Torque teno virus, medicine.medical_specialty, Arthritis, Infectious, business.industry, Arthritis, Middle Aged, Viral Load, medicine.disease, DNA Virus Infections, Rheumatology, Internal medicine, Medicine, Humans, Pharmacology (medical), In patient, Female, business, Aged
Published
2007

7. Daily Administration of Interferon Alpha 2b and Utility of Combination Therapy with Ribavirin as Initial Treatment for Chronic Hepatitis C: Follow up of Patients

Author

G. Maltinti, P. Da Massa Carrara, Mauro Bendinelli, S. Petruccelli, Francesco Costa, M. L. Vatteroni, Massimo Bellini, Pietro Ciccorossi, Angelo Ricchiuti, P. Arpe, Santino Marchi, and Fabrizio Maggi

Subjects
medicine.medical_specialty, Hepatology, Combination therapy, business.industry, Ribavirin, Gastroenterology, Alpha interferon, chemistry.chemical_compound, Chronic hepatitis, chemistry, Internal medicine, medicine, Initial treatment, business, Administration (government)
Published
2000

8. Hepatitis C virus infection: prevalence in psoriasis and psoriatic arthritis

Author

E, Taglione, M L, Vatteroni, P, Martini, E, Galluzzo, F, Lombardini, A, Delle Sedie, M, Bendinelli, G, Pasero, W, Bencivelli, and L, Riente

Subjects
Arthritis, Psoriatic, Prevalence, Humans, Psoriasis, Enzyme-Linked Immunosorbent Assay, Hepacivirus, Hepatitis C Antibodies, Hepatitis C
Abstract

To study the prevalence of hepatitis C virus (HCV) infection in 2 groups of patients, one group with psoriasis and the other with psoriatic arthritis (PsA).We detected anti-HCV antibodies by ELISA and by a recombinant immunoblot assay (RIBA) in the sera of 50 patients with psoriasis and 50 with PsA. As controls we used a group of 76 patients with rheumatoid arthritis (RA), and referred to data on the prevalence of HCV in the general Italian population.By ELISA, anti-HCV antibodies were detected in 6/50 (12%) patients with PsA, in 5/50 (10%) patients with psoriasis, and in 4/76 (5.2%) patients with RA. All the reactive PsA and RA sera also tested positive on RIBA, while only 3 of the 5 positive results for sera of patients with psoriasis were confirmed by RIBA. The prevalence of HCV infection in patients with psoriasis was not significantly higher than in controls. In contrast, the rate of HCV infection observed in the 50 patients with PsA was higher than that in the other groups, the difference being statistically significant between patients with PsA and the general population.Our data do not support the hypothesis that HCV infection may play a role in the pathogenesis of psoriasis. On the other hand they show a statistically significant difference between the prevalence of HCV infection in patients with PsA and the general population.

Published
1999

10. Lymphocyte Subsets in Hemodialyzed HCV+ and HCV– Patients

Author

L Pellegrini, F Bruschi, S Cucciaioni, L Giombini, U Buoncristiani, M L Vatteroni, and P Tacconi

Subjects
Adult, Male, Hepacivirus, MEDLINE, Virus, Renal Dialysis, medicine, Humans, Aged, Uremia, Immune status, biology, business.industry, Hepatitis C, Middle Aged, biology.organism_classification, medicine.disease, Virology, Lymphocyte Subsets, Immunology, Female, business, Lymphocyte subsets
Abstract

The aim of our study was to get information on the immune status of patients who undergo haemodialysis in the presence of an HCV infection. This virus infects not only hepatocytes, but also blood mononuclear cells, in particular B cells [1], but we do not know the role of this lymphocyte involvement in the maintenance and progression of hepatitis. The impairment of the cell-mediated immune response in uraemic patients is well known, for example only 50–60% of uraemic patients undergo a seroconversion after HBV vaccine, compared to 95% of the normal population. Paradoxically the immunodeficiency is concomitant with an activation of related cells which is increased rather than ameliorated by the dialysis treatment [2]. In these patients lymphocytopenia is also present with a reduction of the absolute number of T lymphocytes which remain normal in percentage. The study was performed on the following groups of subjects.

Published
1998
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11. Subject Index, Vol. 77, 1997

Author

A.S. Graphodatsky, G. Hardiman, J.A. Squire, L. Frönicke, P. Bosco, N. Ceratto, S. van Beersum, J.A.M. Graves, H.N. Seuánez, X. Estivill, N. Archidiacono, G. Novelli, G.G. Karpova, C.H.M. Mellink, E. Jenkins, M. Stacey, T. Fujiwara, M. Lovett, R. Knippers, R. Moyzis, M. Jeanpierre, P. Maccarone, J. Wienberg, M. Rocchi, C. Bendixen, S. Weremowicz, B.P. Chowdhary, Y. Yang, A. Pandita, M. Lachtermacher, N. Matas, K.-H. Lee, N. Miyasaka, T. Katagiri, F. Pelliccia, M.-J. Pébusque, R. Godbout, M. Gersh, R. Leube, F. Ugolini, M. Kai, S.-T. Lee, O. Miura, H.G. Brunner, O.V. Cheryaukene, K. Ohsugi, A. Tanzariello, M.A. Ferguson-Smith, A. Geurts van Kessel, P.C.M. O’Brien, W.G. Nash, A. Baldini, T.J. Robinson, M. Matsumoto, A. Pizzuti, Y. Nakamura, M. Mannens, V. Romano, A. Musio, J. Widmer, Déborah Bourc'his, A. Botta, K. Rojas, M.Z. Limongi, L.A. James, A.F. Davies, M. de Meulemeester, R.M. Brunner, C.H. van Os, J.H. Xia, T.K. Watanabe, E.M. Ladenburger, S.-H. Park, C. Rodellar, M. Pritchard, J.L. Kissil, A.S. Hewson, A. Arai, K.N. Sastry, P.M.T. Deen, P. Eydoux, J.A. McMahon, F.C. Canavez, L. Langbein, R. Toder, G. von Beust, M. Schmid, R. Houlgatte, T. Takahashi, P.A. Voûte, N.V. Vorobieva, J. Overhauser, R. Stanyon, E.I.B. Peerschke, K. Yamamoto, J.P. Park, T.K. Mohandas, S. Feo, C. Zijlstra, N.P. Mertvetsov, M. Steenman, U.W. Kenkare, S.A. Wilcox, J. Guimera, P. Zaragoza, N.A. de Haan, W.Y. Hung, J. Ragoussis, D. Birnbaum, M. Ogawa, H. Kobayashi, W. Engel, F. Shimizu, B. Hoebee, B. Ghebrehiwet, T. Ikeuchi, M. Chaffanet, W.R. Harrison, T. Goldammer, S. Ishikawa, E. Burt, K. Wiesmeijer, V. Jurecic, S.D. Pack, M.R. Koehler, B. Beatty, M. Nagata, E. Takahashi, J.F. Bazan, N. Lynch, M. Schwerin, Y. Yokoyama, G. Mirza, A. Fratello, D. Grady, D. Molina Gomes, F. Saito-Ohara, N. Hoggard, C. Dobkin, H. Scherthan, H. van Bokhoven, L.D. Matyakhina, R. Meneveri, A.P. McMahon, H. Murer, R. Marzella, K. Okui, J. Kissing, A. Risch, J.M. Varley, X.-L. Yao, M. Carter, X.-X. Zhang, H.S. Tenenhouse, S. Kurata, O.L. Serov, P.M. Borodin, M. Nadal, M.L. Filipenko, A. Rocchi, M. Kool, W. Schwaeble, H. Hayes, Y. Takei, H. Levéziel, S.J. O’Brien, P. Thygesen, C.H. Fan, H.X. Deng, M. Suzuki, H. Hameister, G.F.M. Merkx, R. Slater, P. Laurent, S. Sebastian, B. Redeker, R.A. Kastelein, E. Viegas-Péquignot, M.A. van Kuijck, L. Xu, M.A.M. Moreira, T. Kozaki, C.C. Morton, M.S. Aly, I.V. Koroleva, H. Kim, E. Sim, A. Ponce de León, A.B. Spurdle, A. Kimchi, A. Simons, G. Rainaldi, Y. Hey, P. Miniou, D. Wells, F.F.B. Elder, P. Riegman, D. Patterson, M. Schepens, S.N. Malchenko, L.A. Witters, L. Viggiano, S. Hirosawa, F. Yang, K.B.M. Reid, C. Elduque, C. Auffray, A.A. Bosma, N. Guo, J.-J. Cassiman, F.O. Fackelmayer, R.J.M. Bindels, A.T. Natarajan, B.-L. Lim, J.B. Searle, As. Ricco, N. Sakuragawa, L. Vatteroni, M.P. Hande, C.K. Ullrich, S. Okuno, T. Siddque, W. Bie, A. Westerveld, Y. Kuga, and B. Dallapiccola

Subjects
Index (economics), Statistics, Genetics, Subject (documents), Biology, Molecular Biology, Genetics (clinical)
Published
1997
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12. Modulation of cytotoxic but not genotoxic effects by dicumarol on mitomycin C treated Chinese hamster cells

Author

Antonio Piras, Fratta D, T. Mariani, Giuseppe Rainaldi, Simi S, A. Moretti, L. Vatteroni, and Gervasi Pg

Subjects
Clinical Biochemistry, Mitomycin C, Biomedical Engineering, Bioengineering, Sister chromatid exchange, Cell Biology, Biology, biology.organism_classification, Molecular biology, Chinese hamster, Staining, chemistry.chemical_compound, chemistry, Toxicity, Immunology, Cytotoxic T cell, sense organs, Cytotoxicity, Methylene blue, Biotechnology
Abstract

can be modulated by dicumarol (DC), a known DTdiaphorase specific inhibitor C21. In order to investigate whether DC can modulate also the genotoxic effects, V79-AP4 Chinese hamster cells were treated with MC and MC + DC. The results showed that dicumarol reduces the toxicity of MC in V79 Chinese hamster cells by modulating the cytotoxic rather than the genotoxic effects. MATERIALS AND METHODS V79-AP4 Chinese hamster cells were grown in Dulbecco’s modified Eagle medium (DMEM) supplemented with 5% Fetal Calf Serum (FCS) at 37 OC in an humidified atmosphere containing 10% CO=. Cytotoxicity assay was performed by treating V79/AP4 cells with MC (Kyowa Hakko Kogyo) in presence or absence of 30 @I DC for 8 hours. After seven days the dishes were stained with methylene blue and scored to measure the residual colony forming ability. For sister chromatid exchange (SCE) induction 5 X 103 cells were treated as described above. BrUdR-labelling and slides preparation and staining were performed in the standard manner. RESULTS

Published
1991
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13. HEPATITIS C VIRUS IN HEMODIALYSIS PATIENTS

Author

P, Rindi, primary, A, Llppi, additional, R, Caprioll, additional, G, Favila, additional, D, Palmarini, additional, R, Baronti, additional, L, Vatteroni M., additional, F, Maggi, additional, and L, Cloel, additional

Published
1996
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14. HEPATITIS C VIRUS IN HEMODIALYSIS PATIENTS

Author

F Maggi, Leopoldo Cioni, M. L Vatteroni, P Rindl, D Palmarini, R. Baronti, R Caprloli, Alberto Lippi, and G. Favilla

Subjects
medicine.medical_specialty, business.industry, medicine.medical_treatment, Hepatitis C virus, Biomedical Engineering, Biophysics, Bioengineering, General Medicine, medicine.disease_cause, Gastroenterology, Biomaterials, Internal medicine, Medicine, Hemodialysis, business
Published
1996
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16. Thymic lymphocytes. II. Phenotypic modifications of thymocytes after concanavalin A stimulation in the presence of interleukin 2: early modifications of Lyt 1+2+ subset and later proliferation of cells with more mature phenotypes

Author

M L, Vatteroni and M, Papiernik

Subjects
Mice, Inbred C57BL, Mice, Phenotype, Isoantibodies, Mice, Inbred DBA, T-Lymphocytes, Concanavalin A, H-2 Antigens, Animals, Interleukin-2, Female, Lymphocyte Activation, Cells, Cultured
Abstract

Cortical thymocytes are devoid of any immune function, as tested by presently available techniques. The ability of this subpopulation to respond to mitogens or antigens in the presence of interleukin 2 (IL-2) produced by activated mature T lymphocytes has been claimed but is still questioned. In an attempt to study the participation of the different thymocyte subsets and especially that of the cortical type, phenotypic modifications were examined during concanavalin A activation in the presence of IL-2. An immunofluorescent double labeling technique with anti-Lyt 1 and anti-Lyt 2 antibodies was used which led to the determination of four different phenotypes: Lyt 1+2+, Lyt 1+2-, Lyt 1-2+, and Lyt 1-2-. Careful analysis of cell viability in culture and expression of the results in absolute numbers of living cells per culture allowed us to follow modifications of small cellular subsets. Cultures of total thymocytes and PNA-agglutinated (enriched in Lyt 1+2+ cells) and non-PNA-agglutinated cells (enriched in Lyt 1+2-, Lyt 1-2+, and Lyt 1-2- cells) were studied. It was shown that thymocyte activation began by early phenotypic modifications which took place within the first 2 hr of culture but only when Con A plus IL-2 were used. These modifications imply the reduction of the Lyt 1+2+ pool and a compensatory enhancement of Lyt 1-2+ and Lyt 1-2- cells, without modification of the total cell number or [3H]thymidine incorporation. These early phenotypic changes are interpreted as the modulation of antigens on the surface of Lyt 1+2+ cells. The second phase of thymocyte activation implies cell death (essentially Lyt 1+2+ cells) and cell proliferation. The cells which specifically proliferate in the presence of Con A and IL-2 are Lyt 1+2- and Lyt 1-2+, the latter always being present in greater number. Cell survival and absolute number of Lyt 1+2- and Lyt 1-2+ cells in the activated PNA- -enriched population are always higher than in total thymocyte and PNA+ cells cultures. Thus, if Lyt 1+2+ cortical thymocytes do not proliferate by themselves, they seem to intervene by providing Lyt 1-2+ cells which proliferate secondarily.

Published
1984

17. Methisoprinol restoration of leukocyte chemotaxis inhibition by blocking factors in immunoproliferative diseases

Author

A, Azzarà, R, Polidori, M, Petrini, M, Margelli, M L, Vatteroni, B, Grassi, and F, Ambrogi

Subjects
Adult, Male, In Vitro Techniques, Middle Aged, Binding, Competitive, Hodgkin Disease, Antibodies, Inosine, Lymphoproliferative Disorders, Chemotaxis, Leukocyte, Inosine Pranobex, Leukocytes, Humans, Female, Waldenstrom Macroglobulinemia, Multiple Myeloma, Aged
Abstract

The ability of methisoprinol to antagonize the serum-inhibiting activity present in the sera of patients affected by immunoproliferative diseases (Hodgkin's disease and multiple myeloma) was investigated. Using a chemotaxis test, it was found that whereas donor leukocytes preincubated in patient sera exhibited impaired chemotaxis, the presence of methisoprinol (500 micrograms/ml) during the preincubation caused a significant recovery of this leukocyte function. Since the results exclude the assumption of a non-specific stimulation of cell motility, two hypotheses appeared possible for the action of methisoprinol: either an antagonistic activity against the blocking factor at the receptor or the metabolic level, or a direct inactivation of this factor. The clinical implications, with regard to the prophylaxis of infections and a better response to chemotherapy, are discussed.

Published
1984

19. Clinical significance of recombinant interferon-α2 neutralizing antibodies in hepatitis patients

Author

F. Maggi, G. Giannelli, L. Roffi, M. Pistello, S. Del Vecchio, M. Currenti, Ferdinando Dianzani, G. Pastore, Guido Antonelli, and L. Vatteroni

Subjects
Hepatitis, Hepatitis C Virus, Interferon, Hepatitis C virus, medicine.medical_treatment, Immunology, Alpha interferon, Interferon alpha-2, medicine.disease_cause, Antigen-Antibody Reactions, Antibody Specificity, Virology, medicine, Humans, Clinical significance, Interferon alfa, Retrospective Studies, biology, Interferon-alpha, Immunotherapy, medicine.disease, Hepatitis C, Recombinant Proteins, Chronic Disease, biology.protein, Antibody, medicine.drug

23. Hypothesis: central nervous system delivery of cyclosporine A for therapy of progressive multifocal leukoencephalopathy.

Author

Focosi D, Kast RE, Maggi F, Vatteroni L, Ceccherini-Nelli L, and Petrini M

Subjects
Animals, Cyclosporine pharmacokinetics, Cyclosporine therapeutic use, Humans, Immunocompromised Host, Immunosuppressive Agents pharmacokinetics, Immunosuppressive Agents therapeutic use, Mice, NFATC Transcription Factors metabolism, Rats, Brain metabolism, Carotid Arteries, Cyclosporine administration & dosage, Immunosuppressive Agents administration & dosage, Leukoencephalopathy, Progressive Multifocal drug therapy
Published
2007
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24. Assignment of Chinese hamster p53 gene (TP53) to chromosome band 2p31, a region not involved in the karyotypic changes of a tumorigenic cell line.

Author

Vatteroni L, Musio A, Meneveri R, and Rainaldi G

Subjects
Animals, Base Sequence, Chromosome Banding, Chromosome Mapping, Cloning, Molecular, Cricetinae, DNA Primers genetics, DNA, Complementary genetics, Humans, In Situ Hybridization, Fluorescence, Karyotyping, Mesocricetus, Molecular Sequence Data, Polymerase Chain Reaction, Sequence Homology, Amino Acid, Tumor Cells, Cultured, Cricetulus genetics, Genes, p53
Abstract

The Chinese hamster tumor-derived cell line 835T2 exhibits specific karyotypic changes, including the loss and/or translocation of genetic material. To investigate whether the p53 tumor suppressor gene was involved in the exchanges, cDNA from primary Chinese hamster cells was isolated by using sense and antisense primers of the human p53 gene. The cDNA was sequenced, and the sequence was compared with the Syrian and human p53 cDNA reported sequences. The sequence homology was very elevated, demonstrating that the cloned fragment contained part of the Chinese hamster p53 gene. The corresponding genomic fragment was also cloned and used as a biotin-labeled probe for in situ hybridization on Chinese hamster chromosome spreads. Hybridization was visualized by avidin-FITC, and the assignment was done comparing the banding obtained with BamHI restriction enzyme and the location of the fluorescent signals pattern of the same metaphase. The signals revealed that the p53 gene (TP53) is localized on Chinese hamster chromosome band 2p31, which is not involved in the karyotypic changes specific to the 835T2 cell line.

Published
1997
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25. Absence of UV-induced non-homologous recombination in repair-deficient CHO cell lines transfected with ERCC genes.

Author

Rainaldi G, Capecchi B, Piras A, and Vatteroni L

Subjects
Animals, CHO Cells radiation effects, Cricetinae, Dose-Response Relationship, Radiation, Humans, Transfection, Ultraviolet Rays adverse effects, Xeroderma Pigmentosum Group D Protein, DNA Helicases, DNA Repair genetics, DNA-Binding Proteins genetics, Endonucleases, Proteins genetics, Recombination, Genetic, Transcription Factors
Abstract

The nucleotide excision repair pathway removes a broad spectrum of DNA lesions, including UV-induced damage. To ascertain whether the repair of the latter has a causative role in the enhancement of non-homologous recombination, Chinese hamster CHO cell lines proficient and deficient in the ability to repair UV-induced damage were transfected with a plasmid containing the bacterial neoR gene. Following UV-treatment an enhancement of non-homologous recombination above the spontaneous level was observed in repair-proficient cells, whereas no increase was observed in repair-deficient cell lines. Hence, the latter were transfected with the corresponding excision repair cross complementing human genes and the resulting repair-proficient transfectants were tested for UV-induced non-homologous recombination. In both untreated and UV-treated transfectants, the frequencies of the event were not significantly different. Cumulatively, the results suggest that non-homologous recombination induced by UV-irradiation is not restored by the correction of the excision repair defect.

Published
1996
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26. dCTP misincorporation in a Chinese hamster mutator phenotype: the role of GGA genetic context.

Author

Rainaldi G, Meneveri R, Mariani L, Ginelli E, Moretti A, and Vatteroni L

Subjects
Animals, Base Sequence, Cell Line, Cricetinae, Cricetulus, DNA genetics, DNA metabolism, Exons, Hypoxanthine Phosphoribosyltransferase genetics, Molecular Sequence Data, Phenotype, Polymerase Chain Reaction, Polymorphism, Single-Stranded Conformational, Deoxycytosine Nucleotides metabolism, Mutation
Abstract

Clone CSA7 is a CHEF18 hamster cell line that shows an increased intracellular accumulation of dCTP. To localize the mutations that accumulate spontaneously in a functional gene of such a mutator phenotype, independent CSA7 mutants of the hypoxanthine-guanine phosphoribosyl transferase (hprt) gene were isolated and screened by a polymerase chain reaction-single strand conformation polymorphism technique. Sixty-two percent of mutants produced detectable changes of the strand migration profile and the mutations were preferentially localized in the exons 3 (31%) and 6 (62%). The sequencing of such exons revealed that the rate of C base incorporation was the major mutation pathway and that the A base of a GGA sequence was the preferential site of misincorporation.

Published
1996
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27. Clinical significance of recombinant interferon-alpha 2 neutralizing antibodies in hepatitis patients.

Author

Antonelli G, Giannelli G, Pistello M, Maggi F, Vatteroni L, Currenti M, Del Vecchio S, Roffi L, Pastore G, and Dianzani F

Subjects
Antibody Specificity, Chronic Disease, Hepatitis C immunology, Humans, Interferon alpha-2, Interferon-alpha immunology, Recombinant Proteins, Retrospective Studies, Antigen-Antibody Reactions, Hepatitis C drug therapy, Interferon-alpha therapeutic use
Published
1994
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28. Prevalence of hepatitis C virus genotypes in Italy.

Author

Pistello M, Maggi F, Vatteroni L, Cecconi N, Panicucci F, Bresci GP, Gambardella L, Taddei M, Bionda A, and Tuoni M

Subjects
Adolescent, Adult, Aged, Aged, 80 and over, Base Sequence, Biopsy, Genotype, Hemophilia A complications, Hepatitis C etiology, Humans, Italy epidemiology, Liver microbiology, Middle Aged, Molecular Sequence Data, Polymerase Chain Reaction, Prevalence, Hepatitis C epidemiology, Hepatitis C genetics
Abstract

Hepatitis C viruses (HCV) present in 110 Italian patients were characterized by genotype-specific PCRs. Among the 65 cases of community-acquired hepatitis, HCV genotype II was dominant (60%), followed by genotypes IV (15%), III (11%), and I (3%). Among the 45 hemophilia-associated cases, the distribution of the four HCV genotypes was markedly different: genotype I was the most prevalent (61%), followed by genotypes II (25%), III (4%), and IV (2%). Double infections were observed in eight patients. Two HCV remained unclassified. For the 45 community-acquired cases from which a liver biopsy was available, genotype II was associated with more severe liver damage than the other types.

Published
1994
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29. Gene amplification and gene deletion are induced at different rates in V79/AP4 Chinese hamster cells.

Author

Vatteroni L, Piras A, Moretti A, and Rainaldi G

Subjects
2-Aminopurine analogs & derivatives, 2-Aminopurine pharmacology, Animals, Aspartate Carbamoyltransferase genetics, Aspartic Acid analogs & derivatives, Aspartic Acid pharmacology, Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) genetics, Cell Division drug effects, Cell Line, Cricetinae, Cricetulus, DNA drug effects, DNA Replication drug effects, Dihydroorotase genetics, Multienzyme Complexes genetics, Phosphonoacetic Acid analogs & derivatives, Phosphonoacetic Acid pharmacology, Drug Resistance genetics, Gene Amplification, Gene Deletion, Mitomycin toxicity, Mutagenesis, Mutagens toxicity
Abstract

The effect of mitomycin C (MMC) treatment on gene amplification and gene deletion induction in a V79/AP4 Chinese hamster cell line was investigated. Spontaneous and induced cellular variants resistant to N-phosphonacetyl-L-aspartate (PALA), which selects for carbamyl-P-synthetase, aspartate transcarbamylase and dihydroorotase (CAD) gene amplification events, and to intermediate concentrations of 2,6-diaminopurine (DAP), which selects for adenine phosphoribosyl transferase (aprt) gene deletion events, were isolated. The molecular analysis of spontaneous and induced PALA- and DAP-resistant clones showed that while in the former the CAD gene was amplified, in the latter both mutations and deletions occurred at the aprt gene. These results indicate that MMC favored the rise of gene amplification rather than gene deletion events, and suggest that aberrant DNA replication processes were responsible for the observed gene amplification.

Published
1993
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30. Analysis of electroporation-induced genetic damages in V79/AP4 Chinese hamster cells.

Author

Vatteroni L, Piras A, Simi S, Mariani L, Moretti A, Citti L, Mariani T, and Rainaldi G

Subjects
Animals, Cell Survival, Cells, Cultured, Chromosome Aberrations, Cricetinae, Cricetulus, Hypoxanthine Phosphoribosyltransferase genetics, Mutation, Polymerase Chain Reaction, Sister Chromatid Exchange, Thioguanine pharmacology, DNA Damage, Electricity
Abstract

Electroporation is a recent technique used to introduce exogenous DNA into eukaryotic cells. It is important to establish that the gene of interest is transferred into a functional, non-mutated recipient cell. V79/AP4 Chinese hamster cells were exposed to high-voltage pulsed electric fields and some biological and genetic effects were measured. The results showed that cytotoxicity was related in a dose-dependent manner to the number of applied pulses. Thioguanine-resistant colony-forming cells as well as chromosomal aberrations were also induced whereas ouabain resistants and sister-chromatid exchanges were not or slightly induced. Spontaneous and electroporation-induced clones that were phenotypically TGR/HATS were used to investigate the hprt locus. Molecular screening of the locus showed that the number of deleted exons was significantly higher in induced than in spontaneous TG-resistant clones, suggesting that the genetic damages induced by electroporation concern the loss of regions well over the size of the hprt locus.

Published
1993
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31. Specific chromosomal aberrations correlated to transformation in Chinese hamster cells.

Author

Simi S, Musio A, Vatteroni L, Piras A, and Rainaldi G

Subjects
Animals, Cricetinae, Cricetulus genetics, Demecolcine pharmacology, Karyotyping, Tumor Cells, Cultured drug effects, Tumor Stem Cell Assay, Cell Transformation, Neoplastic genetics, Chromosome Aberrations
Abstract

Cytogenetic changes were investigated during the spontaneous progression of CHEF18 Chinese hamster cells towards tumorigenicity. We further report the chromosomal characterization of a series of spontaneous anchorage-independent clones, as well as of a series of tumor-derived cell lines resulting from injection of late passage cells in nude mice. The high karyotypic homogeneity (presence of four marker chromosomes strictly associated in all the metaphases analyzed) in all clones and tumor-derived cell lines prompted us to alter the specific pattern of chromosomal aberrations in order to identify which if any of the aberrations were more strictly related to transformation. For this purpose we treated a tumor-derived cell line with Colcemid and analyzed the reversion of anchorage-independent phenotype in the subclones showing an altered association of the four marker chromosomes. We conclude that two of four marker chromosomes contribute to anchorage independence.

Published
1992
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32. Responsiveness of tumorigenic and non-tumorigenic CHEF18 Chinese hamster cells to 1-beta-D-arabinofuranosylcytosine treatment.

Author

Rainaldi G, Pinto B, Mariani T, Vatteroni L, and Piras A

Subjects
Animals, Cell Cycle, Cells, Cultured, Cricetinae, Cricetulus, DNA biosynthesis, Cell Transformation, Neoplastic, Cytarabine toxicity, Sister Chromatid Exchange drug effects
Abstract

In cultured mammalian cells, sister chromatid exchanges are easily induced by agents that perturb the scheduled timing of DNA replication. In this work a blockage of DNA synthesis induced by 1-beta-D-arabinofuranosylcytosine was applied to non-tumorigenic and tumorigenic CHEF18 Chinese hamster cells, and their responsiveness was compared. The data show that both the induction of sister chromatid exchanges and the reduction of the colony-forming ability were less extensive in non-tumorigenic than in tumorigenic CHEF18 cells. The results suggest that a tight control of the scheduled timing of DNA replication is present in non-tumorigenic CHEF18 cells and perhaps this feature avoids the generation of those chromosomal structures that are responsible for the abnormal induction of sister chromatid exchanges and for the elevated cytotoxicity seen in tumorigenic cells.

Published
1992
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33. Folate-sensitive fragile sites in Chinese hamster cell lines.

Author

Simi S, Vatteroni L, Piras A, Mariani T, and Rainaldi G

Subjects
Animals, Cell Line, Chromosome Banding, Chromosome Fragile Sites, Cricetinae, Cricetulus, Karyotyping, Methotrexate toxicity, Chromosome Fragility, Folic Acid pharmacology
Abstract

The expression of fragile sites in three different Chinese hamster cell lines was studied. Results showed that folate-sensitive fragile sites were expressed in the pericentromeric regions of chromosomes 1, 3, 4, 6, and 7 and in band 1q22. A comparison of the breakpoints involved in formation of chromosome rearrangements in some established Chinese hamster cell lines was also made. Results showed that while the specific type of rearrangement was random, the breakpoints were not. Three of the chromosomal sites most frequently involved in breaks were regions in which fragile sites were expressed.

Published
1990
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