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This paper presents a brief overview of the results of the original researches of biological responses induced by agents of cytostatic and regulatory actions in small doses, as well as weak electromagnetic radiation of different frequency bands. The possibility of obtaining the expressed antitumor, antistress and geroprotective effects has been shown. The question of the relation of system mechanisms of realization of these effects with structural rearrangements in biological fluids, as well as the promising directions for optimizing the therapeutic properties of the informational impacts are discussed.

Changes in transplanted sarcoma 45 tissue in outbred albino rats with tumor regression under the effect of magnetite nanoparticles (magnetic fluid) were studied by light and electron microscopy. The ultrastructure and cell death types in regressing tumors and signs of cell-cell interactions with participation of macrophages, lymphocytes, neutrophils, and degranulating mast cells were described. Some possible mechanisms of a pronounced antitumor activity of magnetite nanoparticles were discussed.

High-frequency oscillations in a pulse wave signal in the range of 1-50 Hz and their relation to differential blood count leucocytes have been investigated. It is shown that the correlation coefficients grow in the frequency range of 1-12.5 Hz between high-frequency oscillations in a pulse wave signal and stab neutrophils, monocytes and segmented granulocytes. The procedure of smoothing the coefficients of harmonic variation has been proposed.

It is shown that general nonspecific adaptational reactions are the basis for mechanism of magnetic field effects at the organism level. The character of changes in organism and its subsystems (pattern of a general nonspecific adaptational reactions) is determined by the type of the reaction developing under magnetic field: stress or unknown before antistress reactions of training, calm activation and enhanced activation. The protection and damages under stress are interwoven, homeostatic parameters go beyond norm. Patterns of antistress general nonspecific adaptational reactions are within norm: for training reaction-within the lower half, calm activation-upper half, enhanced activation-somewhat above norm. The induction and frequency dependence of the reactions is periodical: as the parameters are changed to a certain coefficient a regular reiteration of general nonspecific adaptational reactions as topologically invariant transformations is observed at different reactivity levels of organism. General nonspecific adaptational reactions of superior reactivity levels (under weak of magnetic fields of lowest frequency) are more favourable, general nonspecific adaptational reactions of low levels have features of strain, desynchronization. Antistress general nonspecific adaptational reactions, especially activation reaction of superior reactivity levels, enhance nonspecific resistance, in contrast to stress without any damages or waste of energy, produce antitumor, protective (in reference to toxic agents and X-radiation) and rejuvenation effects, promoting self-organization. Regularities of general nonspecific adaptational reactions correspond to complex unequilibrium systems behavior.

Mental stress, and negative emotions forming physicoemotional stress, lead to disturbances of the immune status or even to the formation of immunodeficiency states [4]. A characteristic feature of stress is hypoplasia of lymphoid tissue of the thymus and lymph nodes. Besides stress, the general nonspecific adaptive response (GNAR) to any extremal or strong stimuli, the GNAR to various stimuli of physiological parameters, namely weak, or threshold strength -the training reaction [2, 3], and stimuli of average strength and the activation reaction [i], differing qualitatively from stress, have been described. During the training reaction moderate functional activity is observed in the thymus and lymphatic system (TLS), and the architectonics of the structures of TLS corresponds to the pattern given in textbooks of histology, whereas in the activation reactions, there is high functional activity, expressed mainly as hyperplasia of lymphoid tissue [5].

Threshold-strength stimulation of the hypothalamus of white rats, under conditions of chronic experimentation, affected the blood cholesterol level differently, depending on the duration of the course of stimulations. The initial effect was to raise blood cholesterol, but after prolonged stimulation (1–1.5 months), the cholesterol content fell, following application of the stimulus, to below the initial level; the greatest changes were in the free cholesterol content. The same type of response was given by different hypothalamic structures. The part played by the hypothalamus in regulation of cholesterol metabolism is discussed.

Hypothalamic stimulation repeated many times over a long period in albino rats produced one of two different effects on the growth of grafted 45 and M-1 sarcomas. If the stimulation preceded the transplantation, the growth of the tumor was suppressed, and the tumor might even be completely absorbed. If stimulation followed the transplantation, tumor growth was intensified. A number of hypothalamic nervous structures were involved.

The authors studied the buffer capacity in the tissues of animals with experimentally induced endocrine disturbances and experiments M-1 and sarcoma 45 by the method of potentiometric titration with the aid of a glass electrode. An increase of the buffer capacity in all the tissues was observed following adrenalectomy. Similar changes were noted in thyroidectomy, castration and tumor growth, i.e., reduction of the buffer capacity, mainly in the skeletal muscle tissue as well as in tissues of the brain, spleen, liver and kidneys. The decrease was even more marked in animals with tumors.

The antitumor effect of common nonspecific adaptation reactions of training and activation, obtained by small doses of adrenaline, was studied in the experiments on white rats with sarcoma 45,3,4-benz/a/-pyrene induced tumors and Biskind ovary tumor. Total resorption of the tumor was noted in all cases (55 rats), in which histological investigation of the thymus, lymph nodes, endocrinous glands and white cell count evidenced the development of the activation reaction, while with the training reaction there was only some retardation of tumor growth (51 rats). When it proved impossible to overcome a "chronic" tumor stress (45 rats), no antitumor effect was gained. Thus, the reactions of training and activation obtained by small doses of adrenaline (at high level of the organism reactivity) were found to increase the antitumor reaction of the organism.

The thyroid status in adaptation reaction: stress and activation was studied in experiments on albino Wistar rats using morphometry and a luminescent spectral analysis. Thyroid function inhibition was obtained in stress manifesting itself in the presence of only one maximum with the wave length of 530 nm in the luminescence spectrum, low values of parameter alpha reflecting the ratio of one- and two-coil nucleic acids, a histogram of distribution with a shift to the left. Thyroid activity stimulation was detected during activation: the luminescence spectrum showed both maximums with the wave length of 530 and 640 nm, high values of parameter alpha; a histogram of distribution was almost symmetrical.

The susceptibility of tumor tissue to therapeutic and smaller doses of chemotherapeutic drugs was assessed on the basis of SH-group inhibition in vitro. As therapeutic dose was decreased, rhythmic changes of tumor susceptibility were observed. In the experiments on tumor-bearing rats, the small dose of chemotherapeutic agent, which in preliminary in vitro experiments proved to be as effective as therapeutic one (1/5 therapeutic dose), was found to cause the same suppression of tumor growth. However, it did not induce leukopenia, lymphopenia, thymus involution or any other manifestations of stress which followed multiple injections of therapeutic doses. The white blood cell and thymus indices in rats, which received the small dose, suggest the development of reactions of training and activation which increase the nonspecific resistance of organism.

Weak electrical stimulation of emotiogenic brain structures was found to lead to the development of different general non-specific adaptation reactions. Stimulation of nucleus lateralis septi was found to evoke mainly the development of activation reaction, while stimulation of globus pallidum caused primarily the development of training reactions. Stress reactions were considerably less frequent in electrical stimulation of both kinds of structures than in the control. This can be explained by small values of the electrical current applied. Morphofunctional activity of thymus lymphatic system depended not only on the type of non-specific reaction but also on the character of the stimulated emotiogenic structure--the functional activity was higher with the stimulation of nucleus lateralis-septi. However, the functional activity of thymus lymphatic system in rats with globus pallidum stimulation was higher than in control rats that were not subject to any stimulation.

The morphofunctional state of lymphatic nodes, thyroid gland and testis have been studied with morphometric, cell photometric and biochemical methods in experiments on adult white rats under the influence of nonspecific adaptation stress and activation reactions caused by small doses of adrenaline (125 mg/kg and 5 mg/kg). The research showed that the changes in the structure and function of the lymphatic organs under stress and activation were markedly different. If stress results in depression of functional state of these organs, the activation reaction, on the contrary, stimulates it.