1. Characterization of the Human Pancreas Side Population as a Potential Reservoir of Adult Stem Cells
- Author
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Peter Heinke, Leonard C. Harrison, Thomas Loudovaris, Esther Bandala-Sanchez, Lily Lee, Alana M. Neale, Wayne J. Hawthorne, Thomas W.H. Kay, François Vaillant, Petra Augstein, Ilia Banakh, Helen E. Thomas, Gaetano Naselli, and L. Jorge Góñez
- Subjects
0301 basic medicine ,Adult ,Male ,Adolescent ,CA-19-9 Antigen ,Ductal cells ,Endocrinology, Diabetes and Metabolism ,Cell Separation ,Biology ,Stem cell marker ,03 medical and health sciences ,Tissue culture ,Islets of Langerhans ,Young Adult ,0302 clinical medicine ,Endocrinology ,Side population ,Internal Medicine ,medicine ,Humans ,AC133 Antigen ,Progenitor cell ,Pancreas ,Side-Population Cells ,Cells, Cultured ,Aged ,Hepatology ,Anatomy ,Middle Aged ,Molecular biology ,Pancreas, Exocrine ,Adult Stem Cells ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Female ,Stem cell ,Adult stem cell - Abstract
OBJECTIVES: The side population (SP) contains cells with stem cell/progenitor properties. Previously, we observed that the mouse pancreas SP expanded after pancreatic injury. We aimed to characterize the SP in human pancreas as a potential source of stem cells. METHODS: Human organ donor pancreata were fractionated into islets and exocrine tissue, enriched by tissue culture and dispersed into single cells. Cells were phenotyped by flow cytometry, and the SP was defined by efflux of fluorescent dye Hoechst 33342 visualized by ultraviolet excitation. Cells were flow sorted, and their colony-forming potential measured on feeder cells in culture. RESULTS: An SP was identified in islet and exocrine cells from human organ donors: 2 with type 1 diabetes, 3 with type 2 diabetes, and 28 without diabetes. Phenotyping revealed that exocrine SP cells had an epithelial origin, were enriched for carbohydrate antigen 19-9 ductal cells expressing stem cell markers CD133 and CD26, and had greater colony-forming potential than non-SP cells. The exocrine SP was increased in a young adult with type 1 diabetes and ongoing islet autoimmunity. CONCLUSIONS: The pancreatic exocrine SP is a potential reservoir of adult stem/progenitor cells, consistent with previous evidence that such cells are duct-derived and express CD133.
- Published
- 2017