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343 results on '"L. Iannuzzi"'

1. Non-surgical management of chronic rhinosinusitis with nasal polyps based on clinical-cytological grading: a precision medicine-based approach

Author

M. Gelardi, L. Iannuzzi, M. De Giosa, S. Taliente, N. De Candia, N. Quaranta, E. De Corso, V. Seccia, and G. Ciprandi

Subjects
Otorhinolaryngology, RF1-547
Abstract

La rinosinusite cronica con polipi nasali (CRSwNP) è una malattia cronica nasosinusale, a eziologia infiammatoria, con significativo impatto negativo sulla qualità di vita dei pazienti. La CRSwNP rappresenta ancora oggi una sfida terapeutica per lo specialista ORL, sia per la comprensione della sua eziopatogenesi, sia per il suo controllo clinico ed è questo è testimoniato dalla alta incidenza di recidiva dopo trattamento. Abbiamo voluto verificare lipotesi che un approccio terapeutico nuovo, standardizzato, e individualizzato sul grading clinico-citologico (clinical-cytological grading CCG) consentisse un miglior controllo dei sintomi della malattia, e di ridurre la necessità di ricorrere alla chirurgia. Abbiamo pertanto reclutato 204 pazienti affetti da CRSwNP, di cui 145 hanno regolarmente assunto la terapia rispettando il protocollo proposto, e 59 pazienti, invece, che non hanno assunto la terapia in modo sistematico e sono stati quindi inclusi come controlli. Dopo 5 anni di trattamento standardizzato, abbiamo notato che 15 pazienti su 145 (10,3%) del gruppo con terapia standardizzata avevano avuto un miglioramento dello staging endoscopico, 61 su 145 (42%) si erano mantenuti costanti, mentre 69/145 (47,5%) erano andati incontro a un peggioramento. Nel gruppo di controllo, invece, i pazienti peggiorati erano ben 49 su 59 (83%), con un peggioramento significativo in termini di grading endoscopico di almeno due classi (p < 0,05). I pazienti e i controlli sono stati successivamente stratificati sulla base del CCG in 3 sottogruppi: pazienti con CCG lieve, moderata e grave. Dopo tale suddivisione in classi, è stato possibile evidenziare che nel gruppo con CCG lieve (n = 27), il 92% dei pazienti manteneva negli anni un trend costante, in assenza di peggioramenti e senza necessità di ricorrere alla chirurgia nei 5 anni di osservazione, mentre nel gruppo di controllo, 1 paziente su 59 (1,6%; p =

Published
2017
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2. Characterization of blood redox status of early and mid-late lactating dairy cows

Author

L. Cigliano, M. Strazzullo, C. Rossetti, G. Grazioli, G. Auriemma, F. Sarubbi, C. Iannuzzi, L. Iannuzzi, and M.S. Spagnuolo

Subjects
lactational status, antioxidant defence, oxidative damage, biomarkers, mediterranean italian buffalo, italian holstein friesian bovine, Animal culture, SF1-1100
Abstract

The effect of the stage of lactation on blood redox homeostasis of bovine and buffalo cows was evaluated. The investigation was carried out on early lactating and mid-late lactating cows, reared in a farm located in Campania (southern Italy). Plasma concentration of α-tocopherol and ascorbate, the total antioxidant capacity (TAC), glutathione peroxidase (GPx), and superoxide dismutase activities were higher (P < 0.01) in mid-late lactating cows, thus suggesting a higher consumption of antioxidants during early lactation. Plasma concentration of protein-bound carbonyls (PC) and nitrotyrosine (N-Tyr), and the level of lipid hydroperoxides (LPO) were higher (P < 0.01) in early lactating cows, thus suggesting that lipid peroxidation and peroxynitrite production are crucial in determining oxidative modifications in plasma. TAC was positively correlated with ascorbate concentration (P < 0.03), and negatively correlated with PC concentration (P < 0.002), and ascorbate was negatively correlated with PC (P < 0.03) in mid-late lactating group. These findings demonstrate that circulating ascorbate plays a major role in preventing protein modifications induced by carbonyls, and that ascorbate scavenging effect is impaired during early lactation. We calculated a protein oxidative stress index as the ratio (PC + N-Tyr)/TAC multiplied by 100, and we found that this parameter was higher (P < 0.0001) in early lactating cows. Therefore, it could be useful for assessing the extent of protein oxidative damage in relation to the whole antioxidant status. Further, we suggest that the LPO/GPx ratio multiplied by 100 might be used as lipid oxidative stress index in lactating cows. This index was higher (P < 0.0001) in early lactating cows, and might represent a standard parameter for evaluating the lipid damage depending on a deficiency of the enzymatic antioxidant defence. These parameters are proposed for a possible effective description of physiological changes associated with lactation.

Published
2014
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3. Cytogenetics in animal production

Author

L. Iannuzzi

Subjects
Animal cytogenetics, Chromosome abnormality, Evolution, FISH-mapping, Animal culture, SF1-1100
Abstract

Cytogenetics applied to domestic animals is a useful biotechnology to be applied in the genetic improvement of livestock. Indeed, it can be used to select reproducers free chromosome abnormalities which are responsible for abnormal body conformation (aneuploidy), lower fertility (balanced chromosome abnormalities) or sterility (sex chromosome abnormalities). Cytogenetics may also be applied to assess environmental pollution by studying animals living in hazardous areas and using them as biological indicators (sentinels). Chromosomes also represent optimal biological structures to study the evolution among related (bovids) and unrelated (bovidshumans) species, especially using comparative FISH-mapping which is one of the most powerful tools to establish the correct order of loci along chromosomes. These comparisons allow us to transfer useful information from richer genomes (human) to those of domestic animals. Moreover, the use of specific molecular markers and the FISH-technique on both mitotic and extended (fiber-FISH) chromosomes, has heralded a new era of cytogenetics, allowing swift extension of genetic physical maps, better anchoring of both linkage and RH-maps to specific chromosome regions, and use in a variety of applications (clinical cases, embryo and sperm analyses, evolution). In this study a brief review of these fields of the animal cytogenetics is presented.

Published
2010
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4. The water buffalo: evolutionary, clinical and molecular cytogenetics

Author

L. Iannuzzi

Subjects
River buffalo, Evolution, Chromosome abnormality, Fertility, Animal culture, SF1-1100
Abstract

Although buffalo population is about 1/10 of that of cattle, buffaloes interest a larger human population, especially in the east countries. For this reason, this species is of great economic importance. Two main species of buffalo are found in the world: the Asiatic (water) buffalo (Bubalus bubalis) and the African buffalo (Syncerus caffer). These two different species have both two different sub-species differing in diploid number but interbreeding within the same genus. The water buffalo, especially the river type (2n=50), is the most important one and a summary of the most important cytogenetic findings obtained until now in this species is reported in this paper.

Published
2010
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5. Mapping fragile-sites in the standard karyotype of River Buffalo (Bubalus bubalis, 2n=50)

Author

D. Di Berardino, J. Rubes, L. Iannuzzi, G.P. Di Meo, V. Peretti, F. Ciotola, L. Ramunno, G. Cosenza, A. Pauciullo, G. Coppola, and D. Nicodemo

Subjects
Genetic diversity, Bubalus bubalis, Fragile sites, Chromosomes, Animal culture, SF1-1100
Abstract

A fragile-site map has been preliminarily established in the standard karyotype of river buffalo (Bubalus bubalis, 2n=50) with the aim of unmasking ‘weak’ chromosomal regions in the karyotype of the species. The majority of the breakages took place in the RBA/RBG-negative bands or at the band-interband regions. The most fragile chromosomes were identified as the inactive X, chromosomes 9 and 8, and the active X, with 42, 32, 31 and 30 breakages, respectively. The 400 breakages were distributed in 106 breaksites (BS), with an average intensity of 4 breaks per chromosomal site; (b) the most fragile bands of the river buffalo karyotype were identified as 9q213 with 24 breaks, band 19q21 with 16; inacXq24 with 15; bands 15q23 and 17q21 with 13; band 13q23 with 12, and so on. Preliminary gene mapping analysis revealed that the closest loci to these fragile sites contain genes such as RASA1 and CAST (9q214), NPR3 and C9 (19q19), OarCP09 (15q24), PLP and BTK (Xq24-q25) and EDNRB (13q22), whose mutations are responsible for severe phenotypic malformations and immunodeficiency in humans and mice, and meat quality in pigs. Further cytogenetic and molecular studies are needed to fully exploit the biological significance of the fragile sites in the karyotypes of domestic animals and their relationships with productive and reproductive efficiency.

Published
2010
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6. Clinical cytogenetics in river buffalo

Author

L. Zicarelli, A. Perucatti, G.P. Di Meo, and L. Iannuzzi

Subjects
river buffalo, reproduction, cytogenetics, Animal culture, SF1-1100
Abstract

While autosomal numeric chromosome abnormalities are phenotipically visible (abnormal body conformation) and easily eliminated during the normal breeding selection, sex numeric abnormalities (including the cases of free-martinism), as well as the structural chromosome aberrations, especially the balanced ones, are more tolerate by the animals (normal body conformation) but are often responsible of low fertility (structural abnormalities) or sterility (sex chromosome aberrations), especially in the females. Although river buffalo (Bubalus bubalis, 2n=50) chromosomes have been characterized......

Published
2011
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7. DNA polymerase alpha inhibition by aphidicolin and fragile site expression in prometaphase chromosomes of the Italian Mediterranean River Buffalo (Bubalus bubalis, 2n=50)

Author

D. Di Berardino, J. Rubes, L. Iannuzzi, G.P. Di Meo, V. Peretti, F. Ciotola, L. Ramunno, G. Cosenza, A. Pauciullo, G. Coppola, and D. Nicodemo

Subjects
Genetic diversity, Bubalus bubalis, Fragile sites, Chromosomes, Animal culture, SF1-1100
Abstract

The present study reports on the expression and localization of “fragile sites” (FS) on prometaphase chromosomes of two groups of river buffaloes (Bubalus bubalis, 2n=50; Mediterranean Italian breed), reared in two different farms, with the aim to characterize chromosome fragility in this species. Totally, 400 aphidicolin induced breakages were identified and localized on the standardized ideogram of the river buffalo karyotype. Preliminary results can be synthesized as follows: (a) aphidicolin showed a remarkable decondensing effect on chromosome structure, enabling further studies at high resolution level; (b) the chromosomal expression of the breakages was not different in the two groups of animals; (c) the most fragile chromosomes were the inactive-X, chromosomes 9, 8 and active-X, showing 42, 32, 31 and 30 breakages, respectively; (d) the breaks were localized in the RBG-negative bands (corresponding to eterochromatic regions) or at the band-interband regions; (e) the chromosomal distribution of the break sites was not random and only partially related to chromosome length. The study is in progress to determine the relative incidence of the fragile sites at chromosomal band level, in order to construct a ‘fragile-site map’ of river buffalo, which could be utilized for genetic improvement programs of the species.

Published
2010
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8. Chromosome fragility in Freemartin cattle

Author

V. Barbieri, L. Iannuzzi, G. P. Di Meo, G. Beneduce, R. Buonerba, S. Albarella, V. Peretti, and F. Ciotola

Subjects
Freemartin Cattle, Genome Stability, Sister Chromatid Exchange, Chromosome Aberration., Animal culture, SF1-1100
Abstract

The aim of the present study was to verify chromosome fragility in freemartin cattle using chromosome aberration (CA) and sister chromatid exchange (SCE) tests. A total of eighteen co-twins were investigated. Fourteen animals were identified as cytogenetically chimeric (2n=60, XX/XY) while 4 were classified as normal. Freemartin cattle showed a higher percentage of aneuploid cells (18.64%) and highly significant statistical differences (P < 0.001) in mean values of gaps (4.53 ± 2.05), chromatid breaks (0.26 ± 0.51), and significant statistical differences (P < 0.005) in mean values of chromosome breaks (0.12 ± 0.43) when compared to 10 control animals from single births (aneuploid cells, 11.20%; gaps, 2.01 ± 1.42; chromatid breaks, 0.05 ± 0.22; chromosome breaks, 0.02 ± 0.14).

Published
2010
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9. Classical and molecular cytogenetic studies in some cattle breeds

Author

G. Succi, L. De Lorenzi, A. De Giovanni, D. Incarnato, G.P. Di Meo, L. Molteni, A. Perucatti, and L. Iannuzzi

Subjects
cytogenetics, chromosomal abnormality, FISH, banding, hypofertility, Animal culture, SF1-1100
Abstract

Numerical autosome aberrations have a few importance in the animal breeding since the carriers show generally abnormal body conformation. For this reason, these abnormalities are systematically eliminated from the animal population by the breeders during the animal breeding. Numerical sex chromosome abnormalities are more tolerate by species, since genes are present in single copy (one of two X-chromosome is genetically inactive), and the carriers show usually normal body conformation. For this reason these abnormalities escape the normal breeding selection.

Published
2010
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10. Comparison of genome stability in two pig breeds by using the sister chromatid exchange (SCE) test

Author

V. Barbieri, L. Iannuzzi, A. Perucatti, G.P. Di Meo, F. Ciotola, and V. Peretti

Subjects
sister chromatid exchange, Casertana breed, Italian Large White breed, genome stability, Animal culture, SF1-1100
Abstract

The sister chromatid exchange (SCE) test has been used to detect genome stability in humans (Chaganti, 1974) and the main livestock species (Ciotola et al., 2004; Di Meo et al., 2000; Di Berardino et al., 1979), and to discover DNA damage caused by a variety of natural and artificial chemical compounds (Iannuzzi et al., 1990).

Published
2010
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11. Nasal disorders and cardiovascular damage: flow-mediated dilation and intima-media thickness as risk parameters

Author

M, Gelardi, R, Giancaspro, L, Iannuzzi, S, Taliente, K, Piccininni, N, Quaranta, G, Bulzis, N, Sasso, R, Carbonara, M M, Ciccone, and M, Cassano

Subjects
Otorhinolaryngology, Risk Factors, Nose Diseases, Humans, General Medicine, Carotid Intima-Media Thickness, Dilatation
Abstract

Nasal disorders and cardiovascular damage: flow-mediated dilation and intima-media thickness as risk parameters.

Published
2022

13. Predictors of survival in patients undergoing cardiac rehabilitation after transcatheter aortic valve replacement (TAVR): a multicenter retrospective study

Author

Franco, Tarro Genta, Claudio, Marcassa, Monica, Ceresa, Simonetta, Scalvini, Laura A, Dalla Vecchia, Maurizio, Bussotti, Gian L, Iannuzzi, Simona, Sarzi Braga, Caterina, Rizzo, Roberto F, Pedretti, Andrea, Giordano, and Maria T, LA Rovere

Subjects
General Medicine
Abstract

To evaluate cardiac rehabilitation (CR)-derived predictors of outcome in patients discharged from rehabilitation after transcatheter aortic valve replacement (TAVR).We retrospectively analyzed data from 232 TAVR patients (aged 82±6 years, 55% females) discharged following an average 3-week residential CR program in the period January 2009 to December 2017. Comorbidities (cumulative illness rated state-comorbidity index, CIRSCI), echocardiography on admission, disability (Barthel Index, BI) and functional capacity (6-min walk distance, 6MWD) at discharge, and maximal training session intensity expressed in METs/min were collected. The end-point was all-cause mortality.74 (32%) deaths occurred at 3-year follow-up. At discharge, non-survivors had a higher comorbidity rate (CIRS-CI 5.2±2.3 vs. 4.1±1.9, p=0.000), higher disability level (BI 80.4±24 vs. 88.8±17, p=0.000), and worse renal function (creatinine 1.6±0.9 vs. 1.2±0.4 mg/dl, p=0.000). They were also more often on diuretics (73% vs. 53.2%, p=0.003) and beta-blocker therapy (73% vs. 57.6%, p=0.042) and had a markedly reduced functional capacity (6MWD 221±100m vs. 265±105m, p=0.001). At multivariate Cox proportional hazards regression analysis, independent predictors of survival at follow-up were lower comorbidity rate, a better preserved renal function, lower use of diuretics, and a higher 6MWD at discharge (Harrell's C = 0.707).Patients attending residential CR after TAVR are very old with significant comorbidity. The overall 3-year mortality rate after CR discharge is high. Our findings suggest the need for individually tailored follow-up care in patients discharged from CR after TAVR to address their residual exercise capacity, comorbidities, and renal function impairment.

Published
2022

14. Disentangling the effects of foliar vs. floral herbivory of leaf-cutting ants on the plant reproductive success of

Author

T, Câmara, X, Arnan, V S, Barbosa, R, Wirth, L, Iannuzzi, and I R, Leal

Subjects
Ants, Fruit, Reproduction, Melastomataceae, Animals, Flowers, Herbivory
Abstract

Flower and leaf herbivory might cause relevant and negative impacts on plant fitness. While flower removal or damage by florivores produces direct negative effects on plant fitness, folivores affect plant fitness by reducing resource allocation to reproduction. In this study, we examine the effects of both flower and leaf herbivory by leaf-cutting ants on the reproductive success of the shrub species Miconia nervosa (Smith) Triana (Family Melastomataceae) in a fragment of Atlantic Forest in Northeast Brazil. We conducted a randomized block-designed field experiment with nine replicates (blocks), in which three plants per block were assigned to one of the three following treatments: undamaged plants (ant exclusion), leaf-damaged plants (ant exclusion from reproductive organs, but not from leaves), and flower + leaf-damaged plants (no exclusion of ants). We then measured flower production, fruit set, and fruit production. Our results showed that flower + leaf-damaged plants reduced flower production nearly twofold in relation to undamaged plants, while flower set in leaf-damaged plants remained constant. The number of flowers that turned into fruits (i.e., fruit set), however, increased by 15% in flower + leaf-damaged plants, while it slightly decreased in leaf-damaged compared to undamaged plants. Contrastingly, fruit production was similar between all treatments. Taken together, our results suggest a prominent role of ant floral herbivory across different stages of the reproductive cycle in M. nervosa, with no consequences on final fruit production. The tolerance of M. nervosa to leaf-cutting ant herbivory might explain its high abundance in human-modified landscapes where leaf-cutting ants are hyper-abundant.

Published
2019

15. Aripiprazole and Delusional Disorder

Author

Gregory L Iannuzzi, Ankita A Patel, and Jonathan T. Stewart

Subjects
Aged, 80 and over, Male, medicine.medical_specialty, Risperidone, Schizophrenia, Paranoid, Delusional disorder, business.industry, Dopaminergic, Aripiprazole, Serotonergic, medicine.disease, 030227 psychiatry, 03 medical and health sciences, 0302 clinical medicine, Schizophrenia, medicine, Humans, Antipsychotic Medications, Psychiatry, business, 030217 neurology & neurosurgery, Psychotic illness, medicine.drug, Antipsychotic Agents
Abstract

Delusional disorder is a relatively rare psychotic illness characterized by delusions with contents that are theoretically possible but highly unlikely, and an absence of the disorganized thought and negative symptoms characteristic of schizophrenia. The illness is rarely studied systematically and most guidance with regard to the treatment derives from case reports and small case series. Antipsychotic medications are the mainstay of treatment, but it is not clear whether any particular agent is more effective than others. We report the case of a patient with delusional disorder who had failed to respond to risperidone but improved markedly with aripiprazole. Aripiprazole may show promise as a treatment for delusional disorder, possibly as a result of its effects on both dopaminergic and serotonergic receptors.

Published
2019

16. Measuring Working Memory Is All Fun and Games

Author

Donald J. Bolger, Michael F. Bunting, Michael R. Dougherty, Jacob B. Buchanan, Gregory Colflesh, Timothy L. Briner, Gregory L. Iannuzzi, Sharona M. Atkins, Vadim Kashtelyan, Eamon Dowling, Sy-Yu Chen, Amber M. Sprenger, J. Isaiah Harbison, and Sydnee Chavis

Subjects
Adult, Male, Task switching, Elementary cognitive task, Adolescent, Experimental and Cognitive Psychology, Task (project management), Young Adult, Cognition, Raven's Progressive Matrices, Arts and Humanities (miscellaneous), Task Performance and Analysis, Humans, Attention, General Psychology, n-back, Working memory, Reproducibility of Results, General Medicine, Memory, Short-Term, Go/no go, Stroop Test, Female, Psychology, Cognitive psychology
Abstract

We developed a novel four-dimensional spatial task called Shapebuilder and used it to predict performance on a wide variety of cognitive tasks. In six experiments, we illustrate that Shapebuilder: (1) Loads on a common factor with complex working memory (WM) span tasks and that it predicts performance on quantitative reasoning tasks and Ravens Progressive Matrices (Experiment 1), (2) Correlates well with traditional complex WM span tasks (Experiment 2), predicts performance on the conditional go/no go task (Experiment 3) and N-back (Experiment 4), and showed weak or nonsignificant correlations with the Attention Networks Task (Experiment 5), and task switching (Experiment 6). Shapebuilder shows that it exhibits minimal skew and kurtosis, and shows good reliability. We argue that Shapebuilder has many advantages over existing measures of WM, including the fact that it is largely language independent, is not prone to ceiling effects, and take less than 6 min to complete on average.

Published
2014

19. Classical and Molecular Cytogenetics of Disorders of Sex Development in Domestic Animals

Author

D.A.F. Villagómez, f, P. Parma, O. Radi, G. Di Meo, A. Pinton, L. Iannuzzi, W.A. King, Department of Biomedical Sciences, University of Guelph, Universidad de Guadalajara, Department of Animal Science, University of Milan, Università degli Studi di Pavia, Partenaires INRAE, ISPAAM, Laboratory of Animal Cytogenetics and Gene Mapping, Consiglio Nazionale delle Ricerche [Roma] (CNR), Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées

Subjects
medicine.medical_specialty, 040301 veterinary sciences, BUFFALO BUBALUS-BUBALIS, XY GONADAL-DYSGENESIS, SERTOLI-CELL DIFFERENTIATION, TRANSCRIPTION FACTOR FOXL2, POLYMERASE CHAIN-REACTION, TESTIS CORD FORMATION, CRITICAL TIME WINDOW, Y-CHROMOSOME, TRUE HERMAPHRODITES, RIVER BUFFALO, [SDV]Life Sciences [q-bio], Aneuploidy, Biology, 0403 veterinary science, Molecular cytogenetics, 03 medical and health sciences, Genetics, medicine, Animals, sex, Sexual Maturation, Disorders of sex development, Molecular Biology, Gene, In Situ Hybridization, Fluorescence, Genetics (clinical), 030304 developmental biology, 0303 health sciences, Sexual differentiation, cytogenetic, Cytogenetics, Chromosome, Karyotype, 04 agricultural and veterinary sciences, medicine.disease, 3. Good health, Animals, Domestic, Cytogenetic Analysis
Abstract

Chantier qualité GA; International audience; The association of abnormal chromosome constitutions and disorders of sex development in domestic animals has been recorded since the beginnings of conventional cytogenetic analysis. Deviated karyotypes consisting of abnormal sex chromosome sets (e.g. aneuploidy) and/or the coexistence of cells with different sex chromosome constitutions (e.g. mosaicism or chimerism) in an individual seem to be the main causes of anomalies of sex determination and sex differentiation. Molecular cytogenetics and genetics have increased our understanding of these pathologies, where human and mouse models have provided a substantial amount of knowledge, leading to the discovery of a number of genes implicated in mammalian sex determination and differentiation. Additionally, other genes, which appeared to be involved in ovary differentiation, have been found by investigations in domestic species such as the goat. In this paper, we present an overview of the biology of mammalian sex development as a scientific background for better understanding the body of knowledge of the clinical cytogenetics of disorders of sex development in domestic animals. An attempt to summarize of what has been described in that particular subject of veterinary medicine for each of the main mammalian domestic species is presented here.

Published
2009

20. Contents Vol. 125, 2009

Author

A. De Bustos, S.M. Tuziak, M. Vítková, L. Liotta, C. Morales, A. Sánchez, R. Pérez, S. Kirsch, A. Soler, L. Iannuzzi, R.G. Danzmann, A.G. Papeschi, S. Iwata, F. Marec, N. Jouve, I. Fuková, M. Teruel, W. Schempp, C. Badenas, G. Burt, A. Perucatti, C. Hodler, L. Armengol, M. Tomita, F. Perfectti, V. Peretti, D. Incarnato, J.-C. Wang, M.J. Acosta, M. Kuramochi, M. Giovannotti, J.P.M. Camacho, R. Habibian, H.K. Moghadam, D. Di Berardino, P. Nisi Cerioni, M.J. Bressa, T. Raudsepp, M.I. Pigozzi, H.C. Hauffe, S. Kubíčková, E. Olmo, A. Hajianpour, B. Chowdhary, J. Cabrero, S. Andrés, F. Ciotola, G.P. Di Meo, V. Caputo, I. Mademont-Soler, M. Milà, J.B. Searle, and Á. Cuadrado

Subjects
Botany, Genetics, Zoology, Biology, Molecular Biology, Genetics (clinical)
Published
2009

21. Contents Vol. 119, 2007

Author

M. Duchoslav, M. Guillaud-Bataille, R. Weikard, A.M. Dutrillaux, R.M. Brunner, P. Parma, B. Dutrillaux, S. Brüderlein, J. Dolezel, S. Ganapathiraju, C. Denis, L.-J. Hsieh, H. Xie, E. Hřibová, T.F. Barth, D.L. Adelson, L. De Lorenzi, C. Richon, Ö. Altug-Teber, S. Toujani, A.A. Schäffer, M.J. Puertas, T.L. Lear, J.E. Womack, M. Cuacos, M. Walter, G.P. Di Meo, S.-L. Shi, T. Goldammer, K.G. Heller, M.D. Mudry, L. Iannuzzi, S.A. Brooks, P. Möller, A. Viardot, E. Bailey, J. Macas, I. Tekesin, S. Wegener, M.N. Miziara, A.C. Brasileiro-Vidal, L. Molteni, D. Incarnato, R.-H. Teng, M.E.J. Amaral, H. Mozdarani, Y.-C. Li, R. Berger, J. Doležel, A. Eggen, A. Bernheim, H. Waxin, M. Bonin, A.J. Lukaszewski, F.-J. Tsai, L.J. Campbell, D.S. Perez, P. Vosough, D.I. Smith, Y.-M. Cheng, A.D. Bolzán, D. Kopecky, P.-P. Liu, C.C. Lin, I. Melzner, D.C. Allen, O. Riess, R. Brunner, R. Agarwala, C.D. James, H.K. Müller-Hermelink, M. González-García, R.R. Lemos, J.M. Vega, S. McAvoy, K. Nieselt, A. Perucatti, S. Debarshi, H. Heilbronner, W. dos S. Soares Filho, S. Popov, M. Volleth, M. Doleželová, A. De Giovanni, M. González-Sánchez, U.A. Mau-Holzmann, C.D. Town, M. Nieves, A. Mohseni-Meybodi, R.N. MacKinnon, M. Guerra, K.N. Mohan, B.S. Rani, M. Rosato, S. Selvam, A. Mader, P. Dessen, J.S. Kadandale, A.P. Moraes, A. Dufke, and H. Stappert

Subjects
Botany, Genetics, Zoology, Biology, Molecular Biology, Genetics (clinical)
Published
2007

24. Chromosomal abnormalities in secondary bovine oocytes matured in vitro up to 48 hours

Author

M. Rubessa, A. Pauciullo, V. Peretti, L. Iannuzzi, L. Ramunno, D. Di Berardino, Rubessa, Marcello, Pauciullo, Alfredo, Peretti, Vincenzo, Iannuzzi, L., Ramunno, Luigi, and DI BERARDINO, Dino

Abstract

Preliminary experiments carried out in our laboratory indicated that—by fertilizing oocytes matured in vitro for 24–32 and 48 h—the resulting blastocyst rates decreased from 22.4 % (24 h), to 14.0 % (32 h) to ‘0’ % (48 h). The aim of this study was investigate upon the variation in the incidence of chromosomal abnormalities occurring in bovine oocytes matured in vitro for prolonged periods of time, i.e. from 24 to 32 to 48 h. Abattoir-derived oocytes were matured in vitro using standard procedures, for 24–32 and 48 h. After incubation, the COCs were treated with ialuronidase (3mg/ml) to eliminate the cumulus cells, swelled in hypotonic (KCl, 0.075 M) for 5–10 min, fixed individually on microscope slides with Carnoy fixative, air dried and stained with 5 % Giemsa. Conventional karyotypes were prepared from 50 matured oocytes for each time of maturation, providing the following results: chromosomal abnormalities, including unreduced diploid metaphases, hypo-haploidy and hypo-diploidy, increased from 12 % at 24 h, to 20 % at 32 h, to 36 % at 48 h. The Chi-square test (with Yates corrections) showed significant differences (P>0.01) in the rate of chromosome abnormalities from 24 to 48 h of maturation as well as from 32 to 48 h, whereas the differences between 24 and 32 h were not significant. These results confirm previous data and provide further evidence that bovine oocytes to be used in IVF programs should not be matured in vitro longer than 24–32 h.

Published
2014

25. The application of a multicolor ZOO-FISH on secondary bovine oocytes showed its potential use for aneuploidy detection

Author

A. Pauciullo, A. Perucatti, A. Iannuzzi, D. Incarnato, V. Genualdo, L. Pucciarelli, M. Rubessa, D. Di Berardino, L. Iannuzzi, Pauciullo, Alfredo, Perucatti, A., Iannuzzi, A., Incarnato, D., Genualdo, V., Pucciarelli, L., Rubessa, Marcello, DI BERARDINO, Dino, and Iannuzzi, L.

Abstract

The female gametes are more susceptible to chromosome segregation errors during meiosis I division and therefore they are the major contributors to the embryo aneuploidies. The evaluation of aneuploidies in bovine oocytes is useful for monitoring the reproductive health of this species and FISH is the main method employed for this purpose. To date only 2–3 chromosomes were simultaneously investigated for aneuploidy detection in cattle oocytes. In this work we propose a multi-color ZOO-FISH by the simultaneous detection of six specific chromosome painting probes on secondary oocytes matured in vitro. Standard procedures were employed for 24 h in vitro oocytes maturation, whereas specific autosomal probes were prepared by microdissection and DOP-PCR using river buffalo mitosis (2n=50). Probes were labelled with spectrum-green and -orange in a second DOP-PCR. Three sequential rounds of FISH were achieved for the same slides. Each round was realized using two probes simultaneously hybridized on MII oocytes with the corresponding first polar bodies (I pb). Slides were counterstained with DAPI in antifade. Digital images were captured in gray-scale and pseudo-colored by the software. Six specific probes, painting 3 out of 5 sub-metacentric river buffalo chromosomes (BBU 1p, 1q, 3p, 3q, 4p and 4q) were sequentially hybridized on BTA secondary oocytes with the corresponding (I pb). The different colors of the probes allowed the identification of six cattle chromosomes (BTA 1, 5, 8, 19, 27 and 28) both on MII and polar bodies evidencing no abnormalities for the investigated cells, but confirming their potential use for aneuploidy detection in bovine oocytes. This result opens further opportunity of investigation for clinical cytogenetic applications also in the other species with difficult CGH karyotype.

Published
2014

26. Sister Chromatid exchange (SCE) test in river buffalo cells treated with Furocoumarins

Author

A. Iannuzzi, A. Perucatti, V. Genualdo, A. Pauciullo, L. Pucciarelli, D. Incarnato, R. Melis, C. Porqueddu, M. Marchetti, L. Iannuzzi, Iannuzzi, A., Perucatti, A., Genualdo, V., Pauciullo, Alfredo, Pucciarelli, L., Incarnato, D., Melis, R., Porqueddu, C., Marchetti, M., and Iannuzzi, L.

Abstract

Cytogenetic test can be very useful to detect chromosome (genome) fragility in both animal and human cells. Furocoumarin derivatives constitute a class of compounds widely investigated for the development of photo-chemotherapeutic drugs and effective in treating many diseases. The interest inside this research field originated from the effectiveness of PUVA therapy, realized by oral or topical administration of a linear furocoumarin (psoralen) followed by irradiation with UVA light, for the treatment of psoriasis and cutaneous T-cell lymphoma. Furocoumarins are also present in Psoralea plants elected to be also used as alternative feed for animals considering that it’s a perennial leguminous and, more important, it’s green during the summer time. In the present study we report the preliminary results obtained using the SCE-test in river buffalo cells exposed in vitro to furocoumarin extracts from a Sardinian population of Posoralea morisana (L.) Stirton (Punta Giglio,). Peripheral blood samples from five young river buffaloes (2 males and 3 females) were incubated at 38 °C for 72 h in presence of different quantities of furocoumarin extracts: 0 (control), 50, 100, 200 and 400 μg/ml. Thirty cells for each cell culture (and furocoumarin dose) were analyzed. Although the cell growth appeared normal in both treated and untreated (control) cells, a significant (P

Published
2014

27. Environmental impact on sheep pastured in some polluted areas of Sardinia island: preliminary results with SCE-test

Author

V. Genualdo, A. Perucatti, A. Iannuzzi, A. Pauciullo, L. Pucciarelli, D. Incarnato, G. Lubrano-Lavadera, N. Solinas, S. Bullitta, L. Iannuzzi, Genualdo, V., Perucatti, A., Iannuzzi, A., Pauciullo, Alfredo, Pucciarelli, L., Incarnato, D., Lubrano lavadera, G., Solinas, N., Bullitta, S., and Iannuzzi, L.

Abstract

Sardinia is the second largest island in the Mediterranean sea. Although it is famous for very beautiful landscapes not only along the coast, there are some polluted areas. A special project supported by the Sardinian Region and involving several research groups is trying to study these areas by using several approaches. In the present study, we report the preliminary results obtained from six sheep herds (Sardinian breed) grazing on natural pasturelands in the vicinity of possible polluted areas. Two herds were located in the Nordern area (industrial and military zones) and four herds were located in the Souther area (military, industrial and mine zones) of the Iceland. Peripheral blood samples were collected from 20 adult sheep (over 4 years) randomly selected from each of six herds grazing around above mentioned areas, as well as from three herds grazing in areas retained far from possible polluted areas and used as control. Two types of cell cultures were performed: without (normal cultures) and with addition of BrdU during the last 28 h: the former, to study the AC-test (chromosome and chromatid breaks), the latter for the SCE-test. In this report only data from SCE-test are reported. A total of 92 sheep from polluted areas and 37 sheep fromcontrol areas were studied.Upon 35 cells studied for each animal, SCE-mean values were 8.65±3.40, 8.10±3.50, 8.05±3.08, 7.42±3.34, 9.28± 3.56 and 8.38±3.29 in the exposed areas, as well as 7.86±3.31 in the control group. Significant increasing (P

Published
2014

29. The 36th American Cytogenetics Conference

Author

F. Pelliccia, M. Gerbault-Seureau, J. Daut, C. Denning, T. Meitinger, G. Fakis, K. Kikuchi, A. Dalski, H.G. Nothwang, N. Vogt, Y. Liu, M. Breen, P. Lichter, B. Malfoy, K.-H. Grzeschik, A. Rocchi, D. Incarnato, H.M. Mitchison, I. Papet, A.L. Loudon, C. Rossier, S. Schneider, B. Pedersen, D. Vogt, E. Chung, C. Menzel, L. Chen, G.A. Wittert, J. Perret, F. Willeke, L. Ferrara, Y. Watanabe, B. Ugele, W. Engel, V. Buckle, K.J. Fowler, S.E. Antonarakis, M.L. Ramírez-Dueñas, S.I. Anderson, M.E. Delany, R. Swanson, H. Turnbull, D. Sheer, T.E. Whitmore, F. Wei, M. Meeks, P. B. Bennett, H. Engel, A. Perucatti, J. Bonfils, A.C. Jelmberg, S.M. van der Maarel, K. Masuda, S. Lok, P.J. Hope, Y. Matsuda, L. French, R. Hawken, Ch. Zühlke, A. Hebinck, T. Lehnert, D.B. Zimonjic, R.H. Martin, E. Schwinger, R.M. Gardiner, M.L. Ayala-Madrigal, F. Liners, J.-P. Fryns, T.M. Skinner, T. Haaf, S.W. Scherer, M.M. Miller, E. Chevret, L. Rießelmann, R. Blevins, M.L. Watson, I.M. Adham, C.S. Haley, G.C. Webb, R. Shamsadin, M. Parmentier, L. Bartoloni, J. Koch, C. Gehrig, H. Hayes, S. Spiden, A.W.I. Lo, M.Z. Limongi, S. Ohl, S. Kollers, J.-L. Blouin, E. McKenna, A.B. Krupkin, J. Bernardino, D.S. Haines, J.L. Holloway, H.H. Ropers, R.M. Hope, C.E. Lofton-Day, A.R. Zinn, J. Wirth, S. Joos, N.C. Popescu, C. Lindbjerg Andersen, C.D. DeLozier-Blanchet, E.P. Cribiu, M. Østergaard, B. Brenig, E.V. Volpi, L. Schibler, B. Hinzmann, A. Krempler, M. Faure, M. Payton, N.K. Moschonas, A. Niveleau, N. Inoue, H.F. Otto, L. De Moerlooze, Q. Shi, B. Nielsen, P. Deloukas, K.H.A. Choo, M.F. Seldin, M. Otaño-Joos, C. Derst, D. Lefrançois, A. Schröer, I. Hansmann, P. Kalitsis, M.F. Maurer, R. Fries, M.T. Boyd, T.J. Quinton, A. Vilain, J.B. Vincent, K.K. Wilgenbus, G. von Beust, G.P. Di Meo, B. Dutrillaux, L. Gaddini, W. Scheurlen, G. Mechtersheimer, S. Doerr, A.L. Archibald, L. Iannuzzi, E. Sim, K. Regemann, R. Athwal, S. Kübart, A. Rosenthal, D.J. Figueroa, N. Tommerup, M.-G. Mattei, H. Shima, D.F.S. Longmuir, A.K. Maiti, J. Williamson, N.L. Lopez-Corrales, S. Boukouvala, and C.P. Austin

Subjects
medicine.medical_specialty, Anthropology, Genetics, Cytogenetics, medicine, Biology, Molecular Biology, Genetics (clinical)
Published
2000

30. 13th International Chromosome Conference

Author

L. Iannuzzi, L. Larizza, A.T. Natarajan, C.A. Redi, W. Hennig, J.H. de Jong, and A.R. Leitch

Subjects
Genetics, Chromosome (genetic algorithm), Biology, Molecular Biology, Genetics (clinical)
Published
1998

33. Comparative FISH mapping of BMPR1B, BMP15 and GDF9 fecundity genes on cattle, river buffalo, sheep and goat chromosomes

Author

A. Farhadi, V. Genualdo, A. Perucatti. S. H. Hafezian. G. Rahimi-Mianji, L. de Lorenzi, P. Parma, L. Iannuzzi, and A. Iannuzzi

Abstract

One of the main goal in livestock genomic projects is the preparation of extensive genomic maps that identify loci affecting economically important traits. For this reason, the aim of this study was to compare several physical maps using bovine artificial chromosomes (BAC) clones containing genes related to fecundity genes. Fluorescent in situ hybridization (FISH) was performed on three gene sequences: growth differentiation factor 9 ( GDF9), bone morphogenetic protein 15 (BMP15) and its type I receptor (BMPR1B). They were studied on cattle (BTA), river buffalo (BBU), sheep (OAR) and goat (CHI) for the first time. GDF9 was assigned to BTA7q22.3, BBU9q24, OAR5q22.3 and CHI7q22.3; BMP15 was assigned to BTAXq31, BBUXq36, OAR/CHIXq24; BMPR1B was assigned to BTA/OAR/CHI6q15 and BBU7q21. All loci were mapped on homologous chromosome and chromosome bands, underling the high degree of chromosome homologies among bovids and extending the cytogenetic maps of these four economically important species.

Published
2013

34. Contents Vol. 102, 2003

Author

H.A. Lewin, G. Pielberg, H.B. Park, V. Russo, R.W. Dunstan, M. Niikura, A. Fu, H. Hayes, C. Moran, J. Womack, S. Kubickova, M.A. Ferguson-Smith, J.L. Williams, J.L. Myka, J. van der Poel, Loren C. Skow, B. van Hest, M. Menotti-Raymond, David L. Adelson, J. Schläpfer, H. Bird, J.R. Garbe, A.A. Schäffer, N. Yasinetskaya, M. Marra, K. Jacobs, C. Elduque, Y. Da, F. Yang, E.G. Cothran, W.J. Murphy, D. Zudova, P. Chardon, V. Amarger, E.P. Cribiu, Y. Zhang, D. Gallagher, R. Rullo, L.B. Madsen, M. Zanotti, L. Krejci, L. Molteni, M. Van Poucke, L. Harman, J.W. Keele, M.M. Binns, C. Genêt, J.N. Derr, J. Schein, B. Backofen, S. Peto, G. Guérin, Donald Miller, F.A. Ponce de León, R. Voß-Nemitz, S. Kiuchi, J.A.M. Graves, S. Cirera, Thomas Faraut, C. Garcia, P.J. de Jong, A.L. Gustafson, O.A. Ryder, W.-S. Liu, R. Stephens, J. Rubes, A. Crisà, S. Braglia, T.L. Lear, C.B. Jørgensen, M.V. Arruga, M.F. Rothschild, H. Uenishi, N.P. Carter, O. Ryder, J.A. Price, N. Iannuccelli, Samodha C. Fernando, C. Baiocco, M. Dunø, K. Benke, L.M. Daniels, J.R. Mickelson, A. Valentini, K.M. Reed, K.E. Murphy, B. Benkel, O.R.P. Bininda-Emonds, G. Brown, H.H. Cheng, D. Milenkovic, B. Koop, B. Brenig, Doris M. Kupfer, S. So, L. Iannuzzi, V. Fillon, Y. Chen, A.T.V. Pillai, M.L. Cox, C.W. Beattie, M.L. Houck, N.E. Raney, C. Drögemüller, N. Bosak, Jillian F. Maddox, L. Fontanesi, T.L. Ward, L.D. Chaves, B.P. Chowdhary, L. Alexander, H.-C. Liu, R. Erlandsson, L. Nanni Costa, C.A. Gill, M. Mattheeuws, T. Bønsdorff, N. Rogalska-Niznik, R. Agarwala, M. Schmid, B. Lama, K.A. Greer, A. Van Zeveren, Y. Meng, G. Rohrer, M. Yerle, J.E. Fulton, M. Breen, L.J. Peelman, C. Li, H. Yasue, B. Fu, H.P. Klinger, R.P.M.A. Crooijmans, V. Cantegrel, E. Bailey, T. Veenendaal, S. Mikawa, V.D. Rilington, S. Marklund, M.C. Savarese, S. Mashima, B.J. Ostroski, I. Szczerbal, H. Fiegler, A.H. Petersen, A. Vignal, D.E. Harry, K. Osoegawa, J. Klukowska, H. Hiraiwa, L. Chemnick, L. Schibler, J. Aldenhoven, A. Jaadar, B.W. Kirkpatrick, C. Hansen, V.A. David, M. Longeri, S.I. Anderson, F. Kasai, E. Scotti, S.S. Moore, G. Bongioni, D. Incarnato, S.J. Valberg, A. Eggen, I.R. Franklin, T. Zharkikh, J. Quackenbush, L.D. Lieto, M. Gautier, C. Marchitelli, Bruce A. Roe, E. Cribiu, K.M. Credille, E.J. Cargill, R. Middleton, M .A.M. Groenen, Y. Palti, O. Rezacova, N. Ramlachan, W. Zimmermann, R. Fries, Terje Raudsepp, J.-T. Jeon, C.W. Ernst, E.A. Ostrander, F. Martins-Wess, Udaya DeSilva, L. Andersson, C.E. Rexroad, A. Winter, C.M. Seabury, Y. Lee, M.N. Romanov, V.H. Nielsen, W. Rens, R.D. Schnabel, M.E. Delany, K. Hemmatian, R. Guyon, S.E. Swanberg, M. van Eckeveld, C. Schelling, B.S.D. Urquhart, A. Galli, P.J. Venta, J.F. Taylor, S. Cornelissen, J.B. Dodgson, K.L. Tsai, K. Sandberg, L. Ferretti, G.P. Di Meo, Rebecca L. Tallmadge, Bhanu P. Chowdhary, W. Bridge, C.R. Farber, C. Penado, Alan Archibald, D. Milan, R. Davoli, T.J. Robinson, H. Fairclough, P.C.M. O’Brien, M. Switonski, F. Galibert, T. Leeb, S.J. O’Brien, T. Hayashi, F.A. Habermann, A. Perucatti, Fares Z. Najar, T. Lear, C. Bendixen, Douglas F. Antczak, B. Thomsen, L. Buttazzoni, J. Aerts, J.E. Swinburne, R. Thomas, X. Guo, S.A. Gahr, M. Fredholm, P.H. Nissen, A. Robic, I. Edfors-Lilja, S. Karamycheva, G. Dolf, L.A. Clark, Y. Lahbib-Mansais, L. Skow, Noelle E. Cockett, G.Q. Fitch, and A.T. Bowling

Subjects
Botany, Genetics, Biology, Molecular Biology, Genetics (clinical)
Published
2003

35. Subject Index Vol. 102, 2003

Author

P.H. Nissen, I. Edfors-Lilja, J. Aerts, R. Thomas, X. Guo, M.A. Ferguson-Smith, L. Molteni, M. Fredholm, B. Koop, P.J. Venta, H. Bird, J.N. Derr, T. Zharkikh, J. Quackenbush, M. Marra, R.W. Dunstan, H.A. Lewin, G. Pielberg, L. Chemnick, L.A. Clark, Y. Lahbib-Mansais, N. Ramlachan, Noelle E. Cockett, A. Fu, H. Hayes, G.Q. Fitch, H. Fiegler, A.T. Bowling, E.P. Cribiu, D. Gallagher, R. Rullo, B. Backofen, J.-T. Jeon, C.W. Ernst, H.P. Klinger, R.P.M.A. Crooijmans, H. Yasue, J.L. Williams, V.D. Rilington, B. Fu, G. Rohrer, H.B. Park, V.A. David, F. Kasai, D.E. Harry, M. Zanotti, V. Amarger, R. Fries, Terje Raudsepp, V. Russo, D. Incarnato, Loren C. Skow, I.R. Franklin, C. Marchitelli, S. Cirera, S.S. Moore, M.C. Savarese, K. Sandberg, S. Kubickova, J.F. Taylor, S. Cornelissen, L. Krejci, B.J. Ostroski, A.A. Schäffer, Thomas Faraut, K. Osoegawa, J. Klukowska, M. Gautier, C. Elduque, S. Mikawa, S. Marklund, Samodha C. Fernando, G. Guérin, Donald Miller, F.A. Ponce de León, A. Jaadar, F. Martins-Wess, C.E. Rexroad, Udaya DeSilva, L. Ferretti, K.L. Tsai, L.B. Madsen, E. Bailey, J. Schläpfer, E.A. Ostrander, G.P. Di Meo, J. van der Poel, Bruce A. Roe, J.L. Myka, E. Scotti, H.H. Cheng, E.G. Cothran, G. Bongioni, L. Andersson, A. Winter, M.E. Delany, W.J. Murphy, D. Zudova, T.L. Ward, C. Baiocco, A. Crisà, Rebecca L. Tallmadge, W. Rens, J. Aldenhoven, Bhanu P. Chowdhary, K.E. Murphy, M.F. Rothschild, R.D. Schnabel, M.L. Cox, J.R. Mickelson, A. Valentini, M. Niikura, V. Fillon, M.V. Arruga, C.M. Seabury, M. van Eckeveld, C. Schelling, C. Genêt, J.W. Keele, B.W. Kirkpatrick, M. Schmid, E. Cribiu, C.R. Farber, C. Li, N. Rogalska-Niznik, Y. Chen, J.A. Price, C. Drögemüller, O.R.P. Bininda-Emonds, L.D. Chaves, M.L. Houck, N.E. Raney, C. Penado, K.M. Credille, Alan Archibald, B. Lama, S.J. Valberg, O. Rezacova, B.S.D. Urquhart, A. Eggen, M. Van Poucke, J.A.M. Graves, L. Iannuzzi, K. Benke, L.M. Daniels, B.P. Chowdhary, G. Brown, M. Yerle, Y. Da, F. Yang, S. Braglia, B. Brenig, M .A.M. Groenen, D. Milenkovic, I. Szczerbal, M. Mattheeuws, A.H. Petersen, Jillian F. Maddox, B. van Hest, W. Zimmermann, J.R. Garbe, R. Stephens, J. Rubes, Y. Lee, J. Schein, L. Alexander, H.-C. Liu, M.N. Romanov, M.M. Binns, M. Dunø, P.J. de Jong, A.L. Gustafson, S. Kiuchi, S.E. Swanberg, Y. Palti, B. Benkel, A. Van Zeveren, Y. Meng, C.B. Jørgensen, A. Galli, K.M. Reed, H. Uenishi, N.P. Carter, V.H. Nielsen, J.B. Dodgson, A.T.V. Pillai, C.W. Beattie, K. Hemmatian, R. Guyon, J.E. Fulton, N. Bosak, R. Erlandsson, A. Vignal, C. Moran, J. Womack, K. Jacobs, O. Ryder, Doris M. Kupfer, S. So, David L. Adelson, Y. Zhang, L. Harman, R. Voß-Nemitz, C. Garcia, K.A. Greer, L.J. Peelman, V. Cantegrel, T. Veenendaal, M. Longeri, S.I. Anderson, A. Robic, S. Karamycheva, R. Middleton, B. Thomsen, N. Iannuccelli, L. Buttazzoni, J.E. Swinburne, L. Fontanesi, L. Nanni Costa, C.A. Gill, H. Hiraiwa, S.A. Gahr, G. Dolf, S. Peto, L. Skow, T.L. Lear, L.D. Lieto, M. Breen, E.J. Cargill, T. Bønsdorff, R. Agarwala, M. Menotti-Raymond, N. Yasinetskaya, P. Chardon, W.-S. Liu, O.A. Ryder, S. Mashima, L. Schibler, C. Hansen, Douglas F. Antczak, S.J. O’Brien, T. Hayashi, F.A. Habermann, A. Perucatti, Fares Z. Najar, T. Lear, C. Bendixen, W. Bridge, D. Milan, R. Davoli, T.J. Robinson, H. Fairclough, P.C.M. O’Brien, M. Switonski, F. Galibert, and T. Leeb

Subjects
Index (economics), Statistics, Genetics, Subject (documents), Biology, Molecular Biology, Genetics (clinical)
Published
2003

36. Il ruolo dell’assistenza continuativa nello scompenso cardiaco dell’anziano

Author

FERRARA, NICOLA, G. Longobardi, G. L. Iannuzzi, M. Capaldo, M. Voza, G. Furgi, F. Cacciatore, M. Varricchio, F. R.e.n.g.o., ABETE, PASQUALE, Ferrara, Nicola, G., Longobardi, G. L., Iannuzzi, M., Capaldo, M., Voza, Abete, Pasquale, G., Furgi, F., Cacciatore, M., Varricchio, and F. R. e. n. g., O.

Published
2000

39. Contents, Vol. 62, 1993

Author

S. Ohta, D. Simon, J.H. Kurth, C.N. Hahn, B.K. May, M.C. Kurth, I. Rodriguez de Ledesma, Y.D. Patel, C. Steinlein, B.D. Harris, J.P. Banga, S. Mazan, B. Weiffenbach, D.S. Gallagher, O.M. Garson, J.E. Womack, Z. Tümer, H. Nishi, H.M. Kozman, E.J.M. Schuurman, T. Sasazuki, N. Aoki, A. Pellicer, H.A. Phillips, A.A.B. Bergen, G.J.B. van Ommen, J.C. Mulley, J.A. Padilla, A. Kato, C. Jeanpierre, N. Roeckel, A.P. Monaco, C. Rudduck, D. Sheer, T.A. Jones, E. Austruy, C. Junien, R. Espinosa, A.M. Ryan, G.R. Sutherland, J.-P. Bachellerie, Ca. Westbrook, M. Schmid, H. Lerach, E. Dietzsch, A. Rabasco, D.F. Callen, L. Sottrup-Jensen, M. Cohen-Salmon, T. Kristensen, M M Le Beau, S. Hirosawa, A.M. McGregor, J.J. Pérez-Regadera, E. Bakker, P. Laslo, J.L. Fernández-García, L.-H. Qu, M.C. Wapenaar, M. Dixon, L.M. Duncan, R.H. Martin, R.I. Barnes, A. Kimura, E.M. Bleeker-Wagemakers, A.M. Bowcock, S.A. Whitmore, N. Tommerup, B.B. Knowles, J. Santos, J.L. Omdahl, W.L. Neuman, M. Guttenbach, A.N. Silahtaroglu, P.S. Barnett, M.-G. Mattéi, L. Iannuzzi, Y. Nakamura, E. Baker, P.J. Diergaarde, G.P. Di Meo, M. Martínez-Trancón, and S. Toyota

Subjects
Botany, Genetics, Zoology, Biology, Molecular Biology, Genetics (clinical)
Published
1993

42. Aneuploidy rates in ‘in vitro’ matured MII oocytes with corresponding first polar body in the Podolian and Maremmana cattle breeds by using dual color fluorescent in situ hybridization (FISH)

Author

A. Pauciullo 1, D. Nicodemo 1, G. Cosenza 1, V. Peretti 2, F. Ciotola 3, G.P. Di Meo 4, L. Ramunno 1, L. Iannuzzi 4, J. Rubes 5, and D. Di Berardino 1

Abstract

This study provides preliminary data for establishing a 'baseline-level' of aneuploidy in the female germ cells of two Italian autochthonous cattle (Bos taurus) breeds, the Podolian and Maremmana, by FISH applied on MII metaphase oocytes with the corresponding first polar body (PB). Painting probes for chromosomes Xcen and 5 were obtained by microdissection and DOP-PCR. COCs from 15 Podolian and 11 Maremmana cows were matured in vitro in TCM199 with 20% FCS, 0.5 ?g/ml FSH and 5 ?g/ml LH. After denudation, the oocytes were individually fixed on slides with 1:1 Carnoy fixative. Totally, out of 396 COCs selected for IVM, 305 reached the MII stage (77.0%); of these, 31 (10.2%) were unreduced. Out of 274 MII reduced oocytes, 200 (73.0%) had visible PB, therefore were FISH analyzed. Aneuploid oocytes were 4 out of 200 (2.0%), 3 were nullisomic and 1 disomic, all for chromosome 5. Premature separation of sister chromatids (PSSC) was found only in two oocytes (1%), one for each breed. At breed level, out of 100 oocytes examined in the Podolian, two were found to be nullisomic and one disomic, all for chromosome 5; out of 100 oocytes examined in the Maremmana, only one oocyte was found to be nullisomic for chromosome 5. Interestingly, chromosome X was never involved in aneuploidy. Totally, the two breeds showed different aneuploidy rates: 3% (Podolian) vs 1% (Maremmana). These preliminary results, together with previous results obtained on the Friesian and Brown breeds, suggest that 'interbreed' differences can be found among the various domestic breeds. This aspect is worth to be further investigated.

Published
2010
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43. Estimation of the baseline level of aneuploidy in germ cells and reproductive health in domestic animals

Author

D. Di Berardino 1, D. Nicodemo 1, A. Pauciullo 1, G. Cosenza 1, L. Ramunno 1, V. Peretti 2, L. Iannuzzi 3, and J. Rubes 4

Abstract

The reproductive health of a given species is strictly related to the karyological integrity of the breeding animals (i.e., absence of chromosomal abnormalities) and to the precision with which the haploid chromosomes are segregated into the male (sperm) and female (oocyte) germ cells prior to fertilization. This assumption is supported by the fact that in mammals more than 70% of the embryonic mortality is attributed to aneuploidies in the germ cells which give rise to genetically unbalanced embryos (monosomic or trisomic) destined to abortion, thus reducing fertility and the reproductive/productive efficiency of the animal production industry. Estimation of the 'baseline' level of aneuploidy in sperm and in in vitro matured oocytes with the corresponding first polar body in domestic animal species and breeds/genetic types is now possible by using the fluorescent in situ hybridization (FISH) technique and chromosome specific painting probes obtained via microdissection and DOP-PCR. Such a 'baseline' level could be used as 'control reference' not only for improving the in vitro production of embryos destined to the animal production industry but also, and more importantly, for monitoring future trends of the reproductive health of the domestic species/breeds engaged in zootechnical productions, especially in relation to the increasing risk of environmental challenges and hazards, such as chemical contaminants in the water, soil, air, micotoxins in the feedstuff, hormone unbalancements, nutritional and dietetical mistakes, and other factors which are known to damage the mitotic and meiotic machinery of the cells.

Published
2010

44. Contents, Vol. 59, 1992

Author

G. Gimelli, N.D. Avent, M. Pecorara, L. Ferrara, K. Inoue, R.L. Eddy, M. Acquila, D. Caprino, Y. Itoh, T. Hori, C. Panarello, B. Dutrillaux, C. MacGeoch, D. Patterson, P.J. Taylor, M.J.A. Tanner, T. Rangel-Figueiredo, M.J. Higgins, N.K. Spurr, M.A. Roberts, V. Natarajan, E. Takahashi, V. Hansson, P.G. Mori, S. Wood, E. Viegas-Péquignot, C.M. Baker, X. Zhu, J.M. Dunn, M. Salminen, L. Galleni, J.S. Simard, L. Iannuzzi, K. Hielscher, J.L. Longmire, D. Bérubé, T. Jahnsen, T. Abe, A. Shiels, C.S. Griffin, R. Stanyon, C.J. Mitchell, H. Adachi, N. Lemieux, D. Ayusawa, A. Becker, F.A. van der Hoeven, K. Ridgwell, G.P. Di Meo, L. Santini, S.K. Mahadevaiah, J.A. Meester, H. Nakazato, N.S-F. Ma, G. Giordano, K. Lundström, T.B. Shows, B.U. Zabel, H. Drabkin, K. Willan, R. Samjaa, K. Zernahle, B.L. Gallie, S. Misawa, P. de Boer, M. Hirsch-Kauffmann, H. Nakagawa, S.F. Lockwood, R. Winqvist, R. Gagné, K. Kashima, L.L. Deaven, R.A. Phillips, J.M.G.M. Schöller, S. Ørstavik, R. Schneider, S.K. Kaneda, M. Laatikainen, A. Tellini, I. Ulmanen, B. Carritt, D. Kieninger, A. Stubbe, T. Seno, E. Schneider-Scherzer, M. Schweiger, U. Mittwoch, G. Contrafatto, U. Walter, A.D. Goddard, J.A. Squire, J.N. Derr, M. Sandberg, J. Inazawa, and M. Schertzer

Subjects
Botany, Genetics, Zoology, Biology, Molecular Biology, Genetics (clinical)
Published
1992

45. Contents Vol. 92, 2001

Author

L. Iannuzzi, C. Broccardo, Y. Mao, J.J.M. van Groningen, W.D. Phillips, J. Wang, J. Rathjen, T. Krieg, D.S. Gallagher, F. Sangiuolo, P. Lichter, M. Wilbrink, P.D. Rathjen, N.W. Richards, K.-M. Debatin, Y. Matsuda, A. Vaiman, K.J. Portbury, P. Perelman, F. Bourgeois, X. Huang, D.L. Gumucio, I. Jentsch, S.S. Bhattacharya, Q. Jiang, K. Ying, I. Matera, A. Rösen-Wolff, C. Mas, K. Ogura, J. Koch, S. Kiuru-Kuhlefelt, C. Auffray, P. Denèfle, R. Allikmets, M. Miettinen, S. Schmutz, M.P. Hildebrand, I. Arnould, C. Prades, R. Sauer, C. Sapienza, R.B. Voyle, F. Yang, M. Gahr, L.J. Coignet, A. Kuroiwa, C.G. Scheuerpflug, N. Serdukova, W.O. Bauer, L.M. Gulluyan, B. Brewer, M. Simonneau, J. Fanburg-Smith, A.S. Graphodatsky, I. Kola, C. Stover, M. Sancandi, J. Dai, W. El-Rifai, Y. Obara, M. Bozzali, M.-D. Devignes, E. Reichenberger, A.G. Marneros, T. Otoguro, B.R. Olsen, A. Botta, C. Drögemüller, A. Mincheva, W. Schwaeble, W. Wang, F. Jakob, S-I. Kawada, L. Naudin, B. Jordan, T. Isobe, M.R. Speicher, G. Palumbo, L. Granjon, S. Knuutila, C. Chelala, A. Bulfone, K. Engel, A. Hoffmann, E. Kater-Baats, H. Kuiper, R. Ravazzolo, G. Gradl, S. Fojo, Z. Zhou, M. Schmid, N. Serakıncı, D. Di Berardino, J. Rubes, I. Ceccherini, K. Matsumoto, B. Dallapiccola, K. Tsuchiya, J.M. Moalic, M.A. Lee-Kirsch, S. Galiègue-Zouitina, G. Merkx, S. Thomas, M.A.J. Weterman, V.T. Volobouev, M. Dean, M. Stumm, G. Novelli, F. Guimiot, N. Tommerup, M. Fredholm, A. Baldini, V. Jurecic, N.B. Atkin, W. Zhao, C. Hansen, W. Nie, G.P. Di Meo, I. Gustavsson, M. van Rooijen, S. Cirera, A.T. Remaley, J. Osorio, S. Imbeaud, R. Cinti, F. Amati, R.-D. Wegner, E. Conti, S. Murray, A. Geurts van Kessel, A. Pizzuti, C.M. Owczarek, K. Friis Henriksen, S. Halford, C. Lafargue, R. Tang, C.Y. Gregory-Evans, S. Cui, C.P. Popescu, P.J. Hertzog, T. Leeb, T. Ogura, P.E. Schaner, M. Kuro-o, Diederik R.H. de Bruijn, S. Shulenin, P. Wieacker, W. Rens, N.A. Jenkins, B. Pedersen, M. Fava, H.R. Treutlein, C. Roumier, M. Berloco, F. Pardo-Manuel de Villena, M. Rosier, P.C.M. O’Brien, N.D. Ebenezer, L. Kutschke, D. Fukushi, E.P. Cribiu, A. Ratti, G. Beck, O. Distl, M.-F. Luciani, S.E. Antonarakis, M. Eleveld, Y. Huang, R.M. Hope, J. Kere, J.A. Kennell, Y. Xie, B.S. Milne, M.A. Ferguson-Smith, S. Neubauer, J. Womack, R. Zoorob, H. Hayes, A. Eggen, N.G. Copeland, B. Levacher, Y.-A. Lee, B. Sicard, M. Bak, S.X. Liang, D.J. Gilbert, G. Stranzinger, E. Paditz, H. Mehenni, R. Wieser, G. Chimini, M.G. Mattei, L.M. Schriml, S. Keindorff, I. Nanda, and M. Shichiri

Subjects
Botany, Genetics, Biology, Molecular Biology, Genetics (clinical)
Published
2000

46. Contents Vol. 90, 2000

Author

H. Hameister, R.H. Martin, Eric D. Green, K. Nayernia, C.M. Tsiapalis, S.I. Anderson, W.J. Schwaeble, Alan Archibald, A. Eggen, H. Hayes, S. Weigend, S. Kubalak, K. Hashimoto, T. Escudero, R. Tanuma, P.A. Robinson, M. Schmid, P. Burfeind, V. Fillon, M.A. Ferguson-Smith, K. Wimmers, M. van Bilsen, M.E. Delany, S. Ikegawa, J.P. Leek, J.L. Doyle, N.A. Jenkins, N. Serdukova, K. Kratochwil, F.J. Charchar, W. Lu, S. Bremer, M.F. Maurer, J. Smith, C. Szpirer, Udaya DeSilva, J. Uedelhoven, S. Sexson, K. Ladjali-Mohammedi, J. Szpirer, H.C. Ardley, A.S. Graphodatsky, S. Munné, L. Carim-Todd, J. Burnside, P.A. Doevendans, G. Liu, J. Grønlund, U. Holmskov, J-M. Buerstedde, D. Conklin, N.G. Copeland, M.R. Speicher, G.P. Di Meo, D.W. Burt, N.A.A. Balatsos, S. A’Hara, F. Deák, T.V. Karamisheva, D.J. Gilbert, D.K. Griffin, S.A. Rose, M. Sano, M. Runte, F. Pitel, P. Laurent, J. Hillel, L. Módis, Q. Shi, T.E. Whitmore, N.G.S. Tan, M. Morisson, C. Steinlein, J. Kaufman, F. Raymond, N. Courtis, P. Zaragoza, A. Schulz, J.A.M. Graves, N. Kleiter, I. Kiss, H. Sheng, T. Haaf, M. Tixier-Boichard, J.H. Calvo, R. Bronsaer, M. Schartl, C. Rodellar, M. Balázs, I. Artner, M. Hoehn, M. Hughes, G. Dekomien, P. van Vooren, Webb Miller, N.S. Zhdanova, M .A.M. Groenen, P.R. Lozano, T. Burke, S. Tascou, T. Liehr, C.M. Stover, M. Escarceller, J. Schleypen, J.-C. Courvalin, U. Claussen, M. Gautier, R. Osta, A. Mäki-Tanila, P. Perelman, T.M. Skinner, K. Krysan, N.M. Astakhova, A.F. Markham, A. Vignal, S. Marcos, D. Sable, H.H.Q. Heng, E. Minc, I. Nanda, P. Liénard, H. Marquardt, H.H. Cheng, R.P.M.A. Crooijmans, R. Kreutz, R. Fries, F. Yang, J. Cohen, P.A. Thomson, R. Zákány, S. Mizuno, M. Guttenbach, Y. Nakamura, M. Svartman, A. Robic, L. Iannuzzi, M. Rivière, T.A. Deisher, S. Muratoglu, M. Sandalinas, M. Hirai, B. Buendia, C. Dixkens, C.V. Beechey, N. Bumstead, L. Sumoy, J. Kusuda, B. Schreiner, R. Gödde, Y. Koshizuka, J.T. Epplen, N.B. Rubtsov, N.L. Lopez-Corrales, A. Law, X. Estivill, M. Samiotaki, and W. Engel

Subjects
Botany, Genetics, Biology, Molecular Biology, Genetics (clinical)
Published
2000

47. Contents Vol. 89, 2000

Author

B. Rosenbusch, M.-T. Bihoreau, J. Satrústegui, J. Browne, B.P. Morgan, C. Niehrs, V.A. Valentine, L.Z. Topol, K. Rader, S.-Y. Li, S. Seino, T. Ono, M.L. Ramírez-Dueñas, K.C Arden, T.-H. Hsu, M. Schmid, S. Rensen, S.H. Park, M.J. Pettenati, H. Yano, W. Van Hul, J.L Vernon, K. Ellington, S. Yonezawa, M.A. Sims, A. Dutra, G. Kandala, S. Paradisi, N.A. Jenkins, F. Gruetzner, C. Dixkens, A.I. Protopopov, N.G. Copeland, A. Glinka, L. Ferretti, H.-U.G. Weier, S. Zabel, Y.-C. Li, S. Sonta, R.A. White, E. Roessler, C. Von Kap-Herr, D. Incarnato, M. Osaki, A. Solans, A.V. Zelenin, E. Petek, G.P. Zambetti, K. Wagner, D.S. Holt, P.M. Richardson, N.D. Rendtorff, T.A. Lister, D. Taruscio, L. Iannuzzi, A. Buck, N.A. Jensen, M.G. Foti, M.A. Hyatt, I. Nanda, M.R. James, P. Doevendans, L. Cai, Y. Du, C.X. George, S. Doerr, M. Athanasiou, G.J.J.M. van Eys, Y. Yokoyama, M.R. Barnes, C.E. Samuel, W. Wuyts, R.S. Bora, M.G. Farquhar, F. Sablitzky, L. Archangelo, M.-G. Mattéi, E.R. Zabarovsky, Q. Zhang, M.-J. Pébusque, M.L. Ayala-Madrigal, L.M. Pasztor, M.-C. Hernandez, R.A. Lersch, S. Yamashita, N. Spurr, G.K. Zoraqi, Y.M. Heng, P.M. Kroisel, M.J. Neat, P. Zambonelli, S. Comincini, R.K. Gupta, G.P. Di Meo, P. Musilová, H. Vissing, Z. Shan, E. Kalm, X. Estivill, S.S. Mann, C. Hansen, I. Hansmann, V. Setaluri, A. Stratil, V.I. Kashuba, R. Davoli, M. Fox, C.C. Lin, H. Egger, J.E. Wiley, Y. Wang, Z. Gu, A. Oohira, A.S. Hill, R. Sanz, V. Falbo, J. Fitzgibbon, P.D. Thomsen, A. Perucatti, J.B. Rattner, G. Merkx, H. Kim, S. Masaki, S. de la Luna, T.L. Harboe, L. Schibler, M.A. Israel, S. Paul, O. Bögler, R.Z. Gizatullin, O.V. Muravenko, B. Brenig, S. Ichikawa, M. Muenke, M.B. Powell, J.-F. Cheng, J. Rubeš, C. Looft, A. Vortkamp, P.C. Burr, T.C. Hart, C. Ramos, N. Tommerup, D.A. Campbell, C.M. Owczarek, B.U. Koelsch, L. Jones, Y. Hirabayashi, K.J. Portbury, S. Aono, C.-Z. Wang, S.D. Field, V. Russo, T. Haaf, P.J. Andres-Barquin, W. Emberger, S. Hirano, C. Lee, A. Kindler-Röhrborn, C. Ayuso, T. Tsukasaki, C. Windpassinger, P.J. Hertzog, A. Kanamori, C. Popovici, A.D. Boyer, M.A. Farwell, N. Foot, L.I. Zon, S. Cepica, D. Birnbaum, A. Silahtaroglu, I. Kola, B. Castiglioni, J. Kohlhase, A. Geurts van Kessel, D.J. Gilbert, M. Yerle, V.W. Sykes, K.E. Murphy, A. del Arco, M. Schneider, E.P. Cribiu, N.K. Rushmere, S.T. Suzuki, S. Breitweser, D.G. Blair, Y.K. Jung, N. Shaikh, Z. Tümer, N. Yokoi, K. Lindpaintner, and D.G. Jo

Subjects
Botany, Genetics, Biology, Molecular Biology, Genetics (clinical)
Published
2000

48. X-Y sperm aneuploidy in 2 cattle (Bos taurus) breeds as determined by dual color fluorescent in situ hybridization (FISH)

Author

D, Nicodemo, A, Pauciullo, A, Castello, E, Roldan, M, Gomendio, G, Cosenza, V, Peretti, A, Perucatti, G P, Di Meo, L, Ramunno, L, Iannuzzi, J, Rubes, and D, Di Berardino

Subjects
Male, X Chromosome, Y Chromosome, Animals, Cattle, Aneuploidy, Spermatozoa, In Situ Hybridization, Fluorescence
Abstract

The present study was undertaken to investigate aneuploidy rates in the sperm populations of 2 cattle (Bos taurus) breeds by using dual color fluorescent in situ hybridization (FISH) with Xcen and Y chromosome-specific painting probes, obtained by chromosome microdissection and DOP-PCR. Frozen semen from 10 Italian Friesian and 10 Italian Brown testing bulls was used for the investigation. For each bull, more than 5,000 sperm were analyzed, for a total of 52,586 and 51,342 sperm cells for the 2 breeds, respectively. The present study revealed - in both breeds - a preponderance of the Y-bearing sperm compared to the X-bearing sperm. Within each breed, a statistically significant variation in the various classes of aneuploidy (XX, YY and XY) was found: differences were found in the Friesian breed among the 3 diploidy classes, and in the Brown breed, among the 3 disomy classes (p0.05) as well as among the 3 diploidy classes (p0.01). However, the 2 breeds did not differ significantly in the overall mean rates of X-Y aneuploidy (disomy + diploidy) which amounts to 0.162% in the Italian Friesian and 0.142% in the Italian Brown. When meiosis I (MI) and II (MII) errors were compared, statistically significant differences (p0.01) were found in the disomy classes and in both breeds, whereas the differences between diploidy classes were not significant. Compared to humans, a lower level of aneuploidy has been found in the domestic species analyzed so far. The present study contributes to the establishment of a baseline level of aneuploidy in the sperm populations of 2 cattle breeds which could be used for monitoring future trends of reproductive health, especially in relation to environmental changes and mutagens.

Published
2009

49. Molecular cytogenetics and comparative mapping in goats (Capra hircus, 2n = 60)

Author

L, Schibler, G P, Di Meo, E P, Cribiu, and L, Iannuzzi

Subjects
Goats, Cytogenetic Analysis, Animals, Chromosome Mapping, Humans, In Situ Hybridization, Fluorescence
Abstract

Few goat genome analysis projects have been developed in the last 10 years. The aim of this review was to compile and update all available cytogenetic mapping data, according to the last goat chromosome nomenclature, as well as human and cattle whole genome sequences. In particular, human regions homologous to most of the FISH-mapped microsatellites were identified in silico. This new goat cytogenetic map made it possible to refine delineation of conserved segments relative to the human and cattle genomic sequence. These improvements did not lead to detection of major new rearrangements within ruminants but confirmed the good conservation of synteny and the numerous intrachromosomal rearrangements observed between goats and humans.

Published
2009

50. Molecular cytogenetics and gene mapping in sheep (Ovis aries, 2n = 54)

Author

T, Goldammer, G P, Di Meo, G, Lühken, C, Drögemüller, C H, Wu, J, Kijas, B P, Dalrymple, F W, Nicholas, J F, Maddox, L, Iannuzzi, and N E, Cockett

Subjects
Sheep, Base Sequence, Cytogenetic Analysis, Quantitative Trait Loci, Animals, In Situ Hybridization, Fluorescence, DNA Primers
Abstract

The development of a completely annotated sheep genome sequence is a key need for understanding the phylogenetic relationships and genetic diversity among the many different sheep breeds worldwide and for identifying genes controlling economically and physiologically important traits. The ovine genome sequence assembly will be crucial for developing optimized breeding programs based on highly productive, healthy sheep phenotypes that are adapted to modern breeding and production conditions. Scientists and breeders around the globe have been contributing to this goal by generating genomic and cDNA libraries, performing genome-wide and trait-associated analyses of polymorphism, expression analysis, genome sequencing, and by developing virtual and physical comparative maps. The International Sheep Genomics Consortium (ISGC), an informal network of sheep genomics researchers, is playing a major role in coordinating many of these activities. In addition to serving as an essential tool for monitoring chromosome abnormalities in specific sheep populations, ovine molecular cytogenetics provides physical anchors which link and order genome regions, such as sequence contigs, genes and polymorphic DNA markers to ovine chromosomes. Likewise, molecular cytogenetics can contribute to the process of defining evolutionary breakpoints between related species. The selective expansion of the sheep cytogenetic map, using loci to connect maps and identify chromosome bands, can substantially contribute to improving the quality of the annotated sheep genome sequence and will also accelerate its assembly. Furthermore, identifying major morphological chromosome anomalies and micro-rearrangements, such as gene duplications or deletions, that might occur between different sheep breeds and other Ovis species will also be important to understand the diversity of sheep chromosome structure and its implications for cross-breeding. To date, 566 loci have been assigned to specific chromosome regions in sheep and the new cytogenetic map is presented as part of this review. This review will also summarize the current cytogenomic status of the sheep genome, describe current activities in the sheep cytogenomics research sector, and will discuss the cytogenomics data in context with other major sheep genomics projects.

Published
2009

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