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In Chile, the demand of edible seaweeds has increased during recent years and Callophyllis variegata is one of the most demanded species. This study summarizes information on phenology, aspects, in vitro culture and vegetative propagation methods for Callophyllis. Results indicate that spore production occurs mainly during winter, and recruitment of new plants appear in nature in the spring. Culture studies indicate that spores presented higher germination and growth rates at 8° C and 10 to 12 μmol m−2 s−1. Furthermore, these results indicate that this species presents a high potential for regeneration from its holdfast. The manipulation of temperature, light and culture medium enhances the regeneration process and growth of Callophyllis in the laboratory.