1. Episodic Secretion of Luteinizing Hormone-Releasing Factor (LRF) in the Human1
- Author
-
Seymour Reichlin and L. Everett Seyler
- Subjects
medicine.medical_specialty ,Pituitary gland ,medicine.drug_class ,Endocrinology, Diabetes and Metabolism ,Biochemistry (medical) ,Clinical Biochemistry ,Radioimmunoassay ,Biology ,Biochemistry ,Endocrinology ,medicine.anatomical_structure ,Hypothalamus ,Estrogen ,Internal medicine ,medicine ,Bioassay ,Luteinizing hormone ,Testosterone ,Hormone - Abstract
To determine the relationship between hypothalamic secretion of LRF and pituitary secretion of LH, LRF activity was estimated by a semi-quantitative bioassay and human LH was measured by radioimmunoassay in samples drawn every 5–10 inin over a 70- to 90-min period from the peripheral blood of 5 human subjects with a variety of states of gonadal function. The LRF activity bioassay was based on changes in plasma radioimmnoassayable LH in ovariectomized, estrogen and progesterone-treated rats after intravenous injection of test substance. The assay is sensitive enough to detect LRF activity in as little as 1 ml of plasma. LH levels showed episodic fluctuations in a normal male, 2 testosterone-deficient men, an estrogen-treated, castrated man, and a normal woman studied in pre and postovulatory periods. Episodic fluctuations in plasma LRF activity also occurred in samples from all subjects. Estimated from a bioassay standard curve of responses to synthetic LRH 15 samples from a normal man contained L...
- Published
- 1974