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The possibility of isolating total esters (ferulen) from Ferula tenuisecta roots has been studied. Optimum conditions for extraction of ferulen are established. The results are used to develop a processing scheme for ferulen isolation. Quantitative determination methods for ferulen are proposed.

The optimal regime of cinaroside extraction from the aerial part of Ferula varia has been found. Aprocessing scheme for obtaining cinaroside has been developed on the basis of these data. Two samples of cinaroside (obtained by the laboratory and semi-industrial methods) are comparable to the well-known medicinal preparation lespenephryl with respect to hypoazotemic action. This fact indicates that Ferula varia is a valuable source of cinaroside. A technology has been developed for producing a new medicinal form of cinaroside in 0.05 g tablets.

The optimal regime for extraction of the total flavonoid fraction from the roots of Pseudosophora alopecuroides has been found and the corresponding technological scheme for the isolation of flavonoids from this medicinal plant has been developed. Acomparative pharmacological investigation showed that both the total flavonoid extract obtained using a semicommercial apparatus according to the proposed technology and a laboratory preparation are comparable in activity with the well-known drug tanacechol based on common tansy flowers. This result shows that Pseudosophora alopecuroides offers an important source of biologically active flavonoids possessing hepatoprotector and biligenic activity. The annular natural resource of roots of this medicinal plant has been evaluated. The qualitative composition of the flavonoids and their total content in various extracts have been compared.

The optimum solvent has been selected for the extraction of the total esters (TEs) from the roots ofFerula tenuisecta. The kinetics of the extraction of the TEs from the raw material have been studied and conditions for the chromatographic purification of the preparation have been determined. The optimum conditions for the separation of the TEs have been found by the method of mathematical planning of experimentation.

Chromatophotocolorimetric and accelerated photocolorimetric methods of determining 8-O-harpagide and harpagide in the epigeal parts of the plantsL. platycalyx andL. setulosus have been developed using 8-O-acetylharpagide as the standard substance.

A method for determining tefestrol inFerula tenuisectaroots, based on its TLC separation from accompanying substances and spectrophotometric determination, was proposed

The compositions of classes and fatty acids inArum Korolkowiitubers are determined. The principal acids are 16:0, 18:1, and 18:2. Triterpenes, 4-monomethylsterol, and sterols are found among the unsaponified substances

Substances of iridoid nature identified as harpagide and 8-O-acetylharpigide have been isolated from the herbAjuga turkestanica. A procedure for the quantitative chromatographic determination of iridoids in the raw material has been developed.

A spectrophotometric method has been developed for the quantitative separate determination of the total flavonones and isoflavones when they are present together in an extract ofThermopsis alterniflora.