Search

A feeding strategy that maintains high content of functional molecules in buffalo milk has been verified by giving Sorghum vulgare as green fodder, but it is not available all year round. The aim of this study was to evaluate the inclusion of former food products (FFPs) containing 87% biscuit meal (nonstructural carbohydrate: 60.1%; starch 14.7;crude protein 10.6), in the diet of buffaloes in terms of: (a) fermentation characteristics through gas production technique; (b) milk yield (MY) and quality; (c) content of some biomolecules and total antioxidant activity. The experiment was performed involving 50 buffaloes divided into two groups: Green group and FFPs group (animals fed Total Mixed Ration with either green forage or FFPs respectively). Daily MY was recorded and milk qualitative analyses were determined monthly for 90 days. Furthermore, fermentation characteristics of the diets were studied in vitro. No significant differences were recorded in feed intake, BCS and MY and quality. Similar in vitro fermentation data of two diets were found, with slight differences in terms of gas production and degradability. During the incubation, kinetic parameters showed a faster fermentation process with the diet of the FFPs group in relation to Green group (p < 0.05). Green group had higher levels (p < 0.01) of γ-butyrobetaine, glycine betaine, l-carnitine and propionyl l-carnitine in milk, whereas no differences were observed for δ-valerobetaine and acetyl l-carnitine. Total antioxidant capacity and iron reduction antioxidant assay were higher (p < 0.05) in the plasma and milk of the Green group. The administration of a diet high in simple sugars, obtained with FFPs, seems to favour the ruminal biosynthesis of some metabolites in milk, such as δ-valerobetaine and acetyl- l-carnitine, similar to green forage administration. Overall, the use of biscuit meal can be an alternative to green fodder when it is not available to ensure environmental sustainability and optimize costs without compromising milk quality.

“Lipari obsidian and Neolithic human communities in the Aeolian islands” is a project aimed at studying the connection between obsidian flows on the island of Lipari and Neolithic populations on the Aeolian archipelago, in Italy. As it is well known, obsidian is of particular interest to trace prehistorical trading patterns; indeed, Lipari obsidian has the widest distribution and has been found in southern France, Dalmazia, Sicily and mainland Italy. The project outputs will give a general vision of both archaeological and volcanological aspects through the stratigraphic and radiometric dating of eruptions which produced obsidian, in relationship with the first phases of the human settlements and row material exploitation. To reach this goal, we are considering both raw materials (geological samples) from different flows and artefacts from Neolithic settlements (archaeological samples) on the Aeolian islands, and performing fission-track dating to get the age of obsidian sources and artefacts. Obtained results are expected to shed some new light on the raw material procurement and on the ability of the Neolithic populations to move from their locations, with particular attention to the consequences of environmental features on the first human settlements on the Aeolian islands.

International audience; Glassy ejecta are associatedto a limited number of impact craters, and yet hold key information about hypervelocity impact processes. Here we report on the discovery of a similar to 650 km(2) impact glass strewnfield in the Central Depression of the Atacama Desert. These cm-sized splash-form objects, that we refer to as atacamaites, are essentially composed of a dacitic glass formed by high-temperature melting of local magmatic rocks, with the addition of a variable iron meteorite contamination, 5 wt.% on average. The most likely nature for the impactor is the IIAB iron group. The fission-track plateau method, on two samples, yielded a mean formation age of 7.83 +/- 0.26 Ma. No associated impact crater has been discovered so far, suggesting it may be a relatively small, km-sized crater. The glassy nature, aerodynamic shapes, elevated formation temperature, and low water content are reminiscent of tektites. However, their small size, heterogeneity, oxidation state, significant contamination by the impactor, and likely more proximal provenance distinguish them from tektites. Atacamaites have no equivalent among the few known terrestrial ejected impact glasses, and increase the intriguing diversity of such products that we propose to name "tektoids".

Background How metabolic impairment leads to cardiac dysfunction in diabetics is unknown. A recent theory, derived mainly from rodent models, involves lipid over-storage to cardiomyocytes. Previous human study demonstrated cardiac steatosis on explanted hearts of diabetics with heart failure. However, this study did not provide any evidence about the effects of diabetes milieu on implanted non-diabetic heart. Purpose We evaluated intramyocyte lipid infiltration in explanted heart of type 2 diabetics with end-stage heart failure. Moreover, we studied the effects of diabetic milieu on myocyte lipid infiltration and cardiac function of non-diabetic implanted hearts in type 2 diabetics one year after heart transplantation (HTx). Methods We conducted a prospective study with a follow-up of 12 months on 88 patients over 18 years of age underwent first HTX. Patients with pre-HTx diabetes duration for at least 6 months were included in the study. Patients with endomyocardial biopsy (EMB) considered positive for rejection, according to International Society for Heart Lung Transplantation (ISHLT), and with post-HTx diabetes were excluded from the study. All patients underwent immunosuppression induction according ISHLT indications. All patients were followed applying internationally accepted patient evaluations (echocardiography and metabolic control) and EMB schedules. EBM from patients without rejection evidences were evaluated for intramyocyte lipid infiltration with oil red-O staining (Or-O). Results The patients were divided in diabetics (44%, age 51.6±7.2 y, diabetes duration 11±3 y) and no-diabetics (56%, 52.1±10.9 y). The patients were matched on the basis of eligibility for a HTx. Seven patients (3 diabetics and 4 no-diabetics) died in hospital. 5 (11%) patients developed post- HTx diabetes. No differences were seen in rejection (12% vs. 10%), infection (9% vs. 10%), renal dysfunction (9% vs. 8%) or mortality (7% vs. 8%). Therefore, the study population included 23 no-diabetics and 22 diabetics. After 1 year, we evidenced an impairment of both sx and dx ventricular function as showed by a significantly reduction of ejection fraction and TAPSE in diabetic patients (Figure-A). Although diastolic function not show significant differences among groups, the E/e' ratio showed lower reduction in diabetics. Or-O evidenced that 91% of diabetic and only 2 of no-diabetic explanted hearts (9%) showed intramyocyte lipid infiltration (Figure-B). Moreover, Or-O of EMB, for monitoring heart transplant during 1 year, evidenced a progressive intramyocyte lipid infiltration in 18 diabetics (81%), whereas none of no-diabetics showed intramyocyte lipid infiltration. Conclusions Our data show that almost all of the explanted diabetic hearts had intramyocyte lipid infiltration. More interesting, we observed that healthy heart transplanted in recipients with pretransplant diabetes were affected early by metabolic disorders leading to intramyocyte lipid infiltration.

Objective. To examine if personalized learning objectives influenced student engagement and if achievement of objectives could be measured from course assignments. Methods. Learners created personalized learning objectives that correlated with their own goals within the context of the course. Using a mixed-methods analysis approach, the influence of these objectives on engagement and evidence of achievement of objectives were examined. Results. Students reported a positive influence of personalized learning objectives on engagement. Additionally, measurement of student progression or achievement of objectives was possible from analysis of the course assignments. Conclusion. Personalized learning is an important educational design for future pharmacists and health care professionals. Creating personalized learning objectives that build on centralized course objectives and connect to a broader context is one way to achieve the goal of an engaged and expanded learning experience.

Low-temperature thermochronology provides information on the timing of rifting and denudation of passive margins, and the Red Sea with its well-exposed, young rift margins is a suitable setting for its application. Here we present new apatite fission-track (AFT) data from Sudan northern hinterland and Red Sea coastal areas. From the former region we obtained ages between 270 ± 2 Ma ad 253 ± 53 Ma, and from the coastal belt between 83 ± 8 Ma and 39 ± 7 Ma. These data prompted a review and comparison with low-temperature thermochronological data from the whole Nubian Red Sea Margin, and a discussion on their implication in assessing the margin evolutionary style. AFT data are available for Egypt and Eritrea as well as apatite (U-Th)/He (AHe) ages for two transects transversal to the margin in Eritrea. Both in Egypt and Eritrea AFT data record a cooling event at about 20–25 Ma (Early Miocene) and an earlier, more local, cooling event in Egypt at about 34 Ma (Early Oligocene). The thermal modeling of the Sudan samples provides an indication of a rapid cooling in Miocene times, but does not support nor rules out an Early Oligocene cooling phase. The re-assessment of new and existing thermochronological data within the known geological framework of the Nubian and conjugate Arabian margins favours the hypothesis that early rifting stages were affecting the whole Gulf of Suez–Red Sea–Gulf of Aden system since the Oligocene. These precocious, more attenuated, phases were followed by major extension in Miocene times. As to the mode of margin evolution, AFT age patterns both in Egypt and Eritrea are incompatible with a downwarp model. The distribution of AHe ages across the Eritrean coastal plain suggests that there the escarpment was evolving predominantly by plateau degradation. Copyright © 2009 John Wiley & Sons, Ltd.

Purpose This study explored new ways to maintain academic integrity for large enrollment, completely online courses. We examined the use of ProctorU as our proctoring strategy with the objectives to identify any implementation challenges and understand the impact of using an online proctor on the student experience. Methods In fall 2013, students were surveyed after each exam. Based on these preliminary findings, ProctorU-related questions were included in the course evaluation administered in spring 2014. A mixed-methods analysis plan was used to examine the results, including quantitative analysis and qualitative analysis of open-ended survey questions. Results In the fall 2013 surveys, 88.95% of students reported being satisfied with their experience using ProctorU. Of those who were unsatisfied, following three emerging themes were identified: took too long to setup, technical difficulties, and personnel issues with proctors. In the spring 2014 course evaluation data, the majority of students rated the experience "good" (57.53%), but a large number of those same students also commented on issues they encountered. Over half of the students indicated that the use of ProctorU would influence their future decision to take another online course, either negatively or positively. Conclusions The question of how to maintain academic integrity with online courses is still an ongoing question, but this project demonstrates that online proctoring does influence the educational experience in ways that must be considered when determining the risk and benefit balance of proctored and unproctored assessments.

In vitro-produced embryos are less viable than their in vivo counterparts. It is known that the developmental speed is a reliable marker of embryo viability. One of the major factors impairing in vitro embryo development is oxidative stress. The aim of the study was to evaluate oxidative stress and lipid peroxidation in bovine in vitro-produced embryos that reached different developmental stages at the end of culture. Abattoir-derived oocytes were matured in vitro in TCM-199 with 15% bovine serum, 0.5mugmL(-1) of FSH, 5mugmL(-1) of LH, 0.8mM L-glutamine and 50mgmL(-1) of gentamicin. Mature cumulus-oocyte complexes (COC) were fertilized in Tyrode's modified medium, supplemented by 5.3 SImL(-1) of heparin, 30muM penicillamine, 15muM hypotaurine, 1muM epinephrine and 1% of bovine serum. Both in vitro maturation and IVF were carried out at 39degreesC and 5% CO(2) in air. After 20 to 22h of gamete co-incubation, presumptive zygotes were denuded and cultured in SOF for 7 days at 39degreesC under humidified air with 5% CO(2), 7% O(2) and 88% N(2) in air. At the end of culture, embryos were assessed according to the stage of development as tight morulae (TM), early blastocysts (eBl), blastocysts (Bl), expanded blastocysts (XBl) and hatched blastocysts (HBl). For each stage of development, an average of 20 embryos were used to determine manganese superoxide dismutase (MnSOD) activity and levels of nitric oxide (NO(2)(-)) and thiobarbituric acid-reactive substances (TBARS). The SOD activity was determined by a colourimetric method (Caraglia M et al. 2011 Cell Death Dis. 2, 150, doi:10.1038/cddis.2011.34) whereas NO(2)(-) and TBARS were measured by a spectrophotometric method (Balestrieri et al. 2011 J. Cell. Physiol. doi:10.1002/jcp.22874). Data were analysed by t-test. Greater (P

We determined the fission track age of Transantarctic Mountain microtektites. The plateau method yielded a formation age of 0.85 ± 0.17 Ma. This age overlaps within error with that of the catastrophic impact that produced the Australasian tektite–microtektite strewn field ca. 0.8 Ma ago. This provides further evidence that Transantarctic Mountain microtektites belong to the Australasian tektite–microtektite strewn field, as previously suggested on the basis of geochemical evidence, Sr–Nd isotope systematics and poorly resolved radiometric data.

We used vitrinite reflectance and mixed layered clay minerals to investigate levels of diagenesis of the Oligocene-Miocene basin developed on the nappes of the Alpine orogen in the Peloritani Mts. (NE Sicily). Paleothermal indicators were integrated with stratigraphic-structural analyses and apatite fission-track and (U-Th-Sm)/He ages to define the late evolutionary stages of the Peloritani Mts. The reconstruction of the paleogeothermal gradient in Oligocene-Miocene times was performed to constrain the burial-thermal evolution. In the southern area, thermal parameters increase with depth suggesting that their evolution is ruled by sedimentary burial. They recorded a decrease in paleogeothermal gradients which marked the evolution of the basin from a fore-arc to a thrust-top setting during convergence-collision. In the northern edge of the basin, higher vitrinite reflectance values indicate that thermal evolution was controlled by tectonic burial related to late Langhian-early Serravallian out-of-sequence thrusting. This overburden was removed by extensional tectonics and/or erosion since late Miocene. The short time span at maximum temperature (

In this work four glasses proposed as reference materials for fission-track dating were investigated. Samples from various localities from southern Bohemia and Moravia have been studied in order to identify the Moldavite-bearing deposit(s) with negligible track annealing. The remaining three glasses, JAS-Gl (Japan), Roccastrada (Italy) and Macusanite (Peru) yield thermally lowered fission-track ages, and need application of correction techniques. Therefore, following the requirement that a standard should fulfil: ‘no corrections should be necessary in obtaining the fission-track age’, they should be excluded. However, we considered that their potentiality as reference materials was worthy to be investigated: accuracy may be greatly improved when various reference samples with characteristics similar to those of real samples are available. All these glasses yielded reproducible plateau ages in agreement with available independent ages. The factors computed using track densities corresponding to plateau age determinations are reciprocally well consistent and substantially agree with those of the FCT and Durango apatites, although the data suggest a slight systematical deviation. The Moldavites least affected by track annealing are those from the Jankov deposit (Middle Miocene): this glass appears the most convincing potential age standard among those studied here. In principle, the studied glasses form a very promising set of reference materials for standardisation of techniques, interlaboratory comparison and age calibration itself.

Uncertainty still persists on the structure and evolution of the West Antarctic Rift System, which is one of the largest extensional provinces on Earth. In this paper, we present results of a combined structural and apatite fission track study of the western shoulder of the rift system, in the western coastal area of the Ross Sea between the Reeves and the Mawson glaciers. Structural data indicate that the onshore fault pattern is dominated by N-S striking right-lateral strike-slip to transtensional fault systems and their related subsidiary fault populations in the damage zones. Thermochronological data support a Cenozoic age for these faults and, in particular, the onset of the oblique rifting event at about 50 Ma, in Eocene times. Apatite fission track analyses also suggest the possible occurrence of an older cooling/exhumation event in Cretaceous times. When integrated with the geological and geophysical data sets available in the literature, our structural and thermochronological data provide further support to the interpretation of Cenozoic oblique rifting in the West Antarctic Rift System as induced by the transfer of dextral shear from the mid oceanic ridge in the Southern Ocean, into the Antarctic Plate interior.