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To analyze the epidemiological characteristics of reported imported malaria cases in Zhengzhou City from 2016 to 2020, so as to provide insights into the management of imported malaria in the city.All data pertaining to cases with definitive diagnosis of malaria in Zhengzhou City from 2016 to 2020 were captured from the National Notifiable Disease Report System and the Information Management System for Parasitic Disease Control in China, including individual demographic data, and malaria onset, initial diagnosis and definitive diagnosis data. All data were descriptively analyzed. The duration from malaria onset to initial diagnosis, from initial diagnosis to definitive diagnosis and from onset to definitive diagnosis was compared among cases. In addition, the diagnoses of imported malaria cases in which definitive diagnosis was made were compared with the reexaminations by Zhengzhou Municipal Malaria Diagnosis Reference Laboratory.A total of 302 cases with definitive diagnosis of malaria were reported in Zhengzhou City from 2016 to 2020, and all were imported cases, with

The aim of this study was to analyze the role of LINC01554 in the pathogenesis of hepatocellular carcinoma (HCC) and explore the potential mechanism through which LINC01554 affects the migration and proliferation of HCC cells.LINC01554 expression in HCC tissues and its link to the prognosis of patients were analyzed by The Cancer Genome Atlas (TCGA) database. Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was carried out to examine LINC01554 levels in 60 cases of HCC clinical tissues and HCC cell lines. Then, LINC01554 overexpression model was constructed using lentivirus in HCC cell lines. HCC proliferation and invasive ability were evaluated through Cell Counting Kit (CCK-8) and transwell tests, respectively. Furthermore, the potential action mechanism of LINC01554 was explored using bioinformatics analysis and in vitro cell experiments.Analysis of the TCGA database revealed that LINC01554 was remarkably under-expressed in HCC tissues. Decreased expression of LINC01554 predicted a poor prognosis for patients. Besides, LINC01554 overexpression markedly blunted the proliferation and migratory capacities of HCC cells. LINC01554 competed with NGFR to bind to microRNA-3681-3p, thereby providing possible mechanisms by which LINC01554 could participate in the progression of HCC.This study shows for the first time that LINC01554 modulates NGFR expression by binding to microRNA-3681-3p, thereby participating in the progression of HCC.

In order to study the comprehensive influence of propeller power and sea surface effect of a seaplane, a composite material variable torque propeller for wind tunnel power simulation was developed. In order to make the quality of wind tunnel test data meet the standard of engineering application and control the quality of propeller products, a quality control method was developed to control the quality of design, checking, manufacturing, verification and operation of the propeller in all stages. In this paper, the research and development process and quality control method of the propeller, tests and the detection results, as well as the application test are described in detail. The results show that the quality control method described in this paper can be used to design and manufacture the propeller products which match the technical indicators, and the power simulation system composed of the propeller can exert slipstream influence on a certain seaplane under various working conditions, so as to meet the requirements of the study on the comprehensive influence of propeller power and sea surface effect of the aircraft.

The characteristics of multidecadal variability (MDV) in global land surface air temperature (SAT) are analyzed based on observations. The role of sea surface temperature (SST) variations in generating MDV in land SAT is assessed using atmospheric general circulation model simulations forced by observed SST. MDV in land SAT exhibits regional differences, with amplitude larger than 0.3 °C mainly over North America, East Asia, Northern Eurasia, Northern Africa and Greenland for the study period of 1902–2004. MDV can account for more than 30 % of long-term temperature variation during the last century in most regions, especially more than 50 % in parts of the above-mentioned regions. The SST-forced simulations reproduce the observed feature of zonal mean MDV in land SAT, though with weaker amplitude especially at the northern high-latitudes. Two types of MDV in land SAT, one of 60-year-timescale, mainly observed in the northern mid-high-latitude lands, and another of 20–30-year-timescale, mainly observed in the low-latitude lands, are also well reproduced. The SST-forced MDV accounts for more than 40 % amplitude of observed MDV in most regions. Except for some sporadically distributed regions in central Eurasia, South America and Western Australia, the SST-forced multidecadal variations are well in-phase with observations. The Atlantic Multidecadal Oscillation and Pacific Decadal Oscillation signals are found dominant in MDV of both the observed and SST-forced land SAT, suggesting important roles of these oceanic oscillations in generating MDV in global land SAT.

Aim We sought to discuss vascular anastomosis and gut reconstruction in a living-related small bowel transplantation recipient. Methods Living-related small bowel transplantation was performed successfully on a boy with short gut syndrome in two stages. In the first stage, 120 cm, of his mother’s ileum was implanted into the recipient with the artery and vein anastomosed to the recipient’s sigmoid artery and inferior mesenteric vein, respectively. The two ends of the implanted intestine were constructed as stomas. In the second stage, reconstruction of the continuity of the digestive tract was performed at 188 days after the initial transplantation. The residual small bowel was transected and both ends were anastomosed to the proximal and distal end of the graft in end-to-side fashion. The stomas were closed 30 and 43 days later. Results Both procedures were successful. Postoperative cytomegalovirus infection and acute rejection occurred successively and were controlled. No leakage of the reconstructed gut or other complications developed after the second procedure. The recipient is alive at 15 months with 8 kg an increase in weight. He is caring for himself independently and has a half-liquid diet, sometimes supplied with auxiliary enteral nutrition. A d -xylose test increased from 4.25% to 25% after the small bowel transplantation. Conclusions Vascular anastomoses should be performed according to the state of graft and the recipient. The portal route is the first choice when possible. A two-stage gut reconstruction could decrease the incidence of complications, and offer a useful method in living-related small bowel transplantation.

Two preparations of glutamate receptors were purified from the synaptic junctions of pig brain by a combination of detergent solubilization, anion-exchange chromatography, wheat-germ agglutinin affinity chromatography and sedimentation through sucrose gradients. These preparations were enriched in specific L-[3H]glutamate binding activity (> 5000 pmol of glutamate binding sites/mg of protein), and the rank order of ligand affinity for binding to these preparations was: quisqualate > 6-cyano-7-nitroquinoxaline-2,3-dione > α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) > L-glutamate > kainate ≫ N-methyl-D-aspartate≈ L-2-amino-4-phosphonobutyrate. SDS/PAGE analysis revealed that more than 80% of the protein in either of these preparations appeared as a single protein band of 106 kDa. Two-dimensional gel electrophoresis further revealed that these 106 kDa proteins consisted of a series of acidic proteins which were recognized by antibodies against rat AMPA receptor subunits. These 106 kDa proteins were also recognized by wheat-germ agglutinin and concanavalin A; in addition, peptide N-glycosidase F treatment of these preparations decreased their size to 99 kDa. Our results suggest that the putative glutamate receptors isolated here are likely to belong to the AMPA subtype of glutamate receptors in pig brain. Using the purification procedure reported here, 5 µg of AMPA receptor proteins can be isolated from 250 g of pig brain tissue.

The study is aim to compare two kinds of cleaning score system for capsule endoscopy, with a view of these two cleaning score system can help to evaluate small bowel cleanliness.Three readers evaluated these two cleaning score system by assessing the inter-observer, intra-patient, and intraobserver agreement.The assessment of the reliability and concordance, inter-observer agreement and intra-patient agreement of System1 and System2 was excellent with the intraclass correlation coefficient (ICC) values of 0.873, 0.821, 0.863 and 0.772. The data regarding the assessment on intra-observer agreement and intrapatient agreement of System1 and System2 were available and the results were also excellent with ICC values of 0.887, 0.846, 0.870 and 0.809. The overall adequacy assessment of System1 and System2, there was no significant difference among the three readers of inter-observer agreement (X2 = 0.051, P = 0.822, X2 = 0.085, P = 0.081, X2 = 0.048, P = 0.827) and intra- patient agreement (X2 = 0.196, P = 0.658, X2 = 0.208, P = 0.648, X2 = 0.054, P = 0.817), neither was intra-observer agreement (X2 = 0.208, P = 0.648, X2 = 0.223, P = 0.637, X2 = 0.484, P = 0.487) and intrapatient agreement (X2 = 0.054, P = 0.817, X2 = 0.054, P = 0.817, X2 = 0519, P = 0.471).The two system both are simple, operable, and can be used in clinical practice.

We report an experimental investigation in the laser-induced damage threshold (LIDT) of optical coatings materials. The samples are single layers of Al2O3, Nb2O5, HfO2, SiO2, Ta2O5, ZrO2 deposited through different deposition techniques (evaporation or sputtering with/without ion assistance) and mixtures of Al2O3/SiO2, Nb2O5/SiO2, HfO2/SiO2, Ta2O5/SiO2 and ZrO2/SiO2 on silica substrates. The LIDT is measured at 1030nm, 500fs in single shot mode. The results are expressed and compared in term of LIDT as a function of bandgap and LIDT as a function of refractive index.

Recent developments in the field of microscopic hardness models have been reviewed. In these models, the theoretical hardness is described as a function of the bond density and bond strength. The bond strength may be characterized by energy gap, reference potential, electron-holding energy or Gibbs free energy, and different expressions of bond strength may lead to different hardness models. In particular, the hardness model based on the chemical bond theory of complex crystals has been introduced in detail. The examples of the hardness calculations of typical crystals, such as spinel Si₃N₄, stishovite SiO₂, B₁₂O₂, ReB₂, OsB₂, RuB₂, and PtN₂, are presented. These microscopic models of hardness would play an important role in search for new hard materials. Зроблено огляд останніх розробок в області мікромоделей твердості. У цих моделях теоретичну твердість описано як функцію щільності та міцності зв’язку. Міцність зв’язку може бути охарактеризована шириною забороненої зони, опорним потенціалом, енергією утримання електрона або вільною енергією Гіббса. Тому різні вирази міцності зв’язку приведуть до різних моделей міцності. Зокрема, докладно описано модель твердості, основану на теорії хімічного зв’язку складних кристалів. Наведено приклади розрахунків твердості типових кристалів, таких як шпінель Si₃N₄, стишовіт SiO₂, B₁₂O₂, ReB₂, OsB₂, RuB₂ и PtN₂. Ці мікромоделі твердості будуть грати важливу роль в пошуку нових твердих матеріалів. Дан обзор последних разработок в области микромоделей твердости. В этих моделях теоретическая твердость описана как функция плотности и прочности связи. Прочность связи может быть охарактеризована шириной запрещенной зоны, опорным потенциалом, энергией удержания электрона или свободной энергией Гиббса. Поэтому различные выражения прочности связи приведут к различным моделям твердости. В частности, подробно описана модель твердости, основанная на теории химической связи сложных кристаллов. Привены примеры расчета твердости типичных кристаллов, таких как шпинель Si₃N₄, стишовит SiO₂, B₁₂O₂, ReB₂, OsB₂, RuB₂ и PtN₂. Эти микромодели твердости будут играть важную роль в поиске новых твердых материалов.

Introduction The aim of this study was to develop a rat model of chronic irreversible rejection, which is a major causes of late graft loss and retransplantation after orthotopic liver allotransplantation. Methods Allogeneic liver transplantation was performed in a rat combination of Dark Agouti (DA) to Brown Norway (BN). Group A was left without treatment, group B received cyclosporine’ (CsA; 1 mg/kg/d) and group C, CsA (4 mg/kg/d). Animals were followed for 6 months. Liver tissue was harvested to construct a time course of histological changes after liver transplantation using histopathological and morphometric techniques. We compared the total histological score of rejection activity index and survival rates. Results In untreated animals, irreversible acute rejection developed, all animals died within 15 days. In the low-dose CsA group, all animals that survived more than 30 days developed moderate to severe manifestations of chronic liver rejection, with graft infiltration, ductular damage or proliferation, obliterative arteriopathy, and liver fibrosis. No apparent histological alterations were observed in group C. Survival analysis showed significant differences between the three groups. Conclusions In the rat strain combination of DA → BN with low-dose immunosuppression, early mild inflammation was followed by the development of chronic rejection.

A series of novel azo dyes composed of a lanthanide complex anion and an azo cation, in which strongly electron-donating (dihexadecylamino)phenyl and electron-accepting pyridinium groups are separated by an azo group, was designed as second-order nonlinear optical Langmuir-Blodgett (LB) film materials. The compounds are of good film-forming properties. The values of second-order molecular hyperpolarizability {beta} were determined to be (1.20-3.03) x 10{sup {minus}27} esu, comparable to the largest value known for azo LB materials. The compounds studied may be attactive in the application in future optical devices. 13 refs., 5 figs., 1 tab.

Comparison studies of recombinant prourokinase(pro-UK) in various host cells, and expression vectors were carried out. Expression levels of vectors constructed in this study in different cell lines were compared. Mammalian cells expressing pro-UK were established. The levels of pro-UK expression in recombinant Namalwa, Vero and Sp2/0 cells are 200, 12.5 and 50 IU/(10(6) cells 24 h). pro-UK purities separated by immunoaffinity chromatograph are above 90%. Immunoabsorbent assay showed the ratio of pro-UK in CHO cells is lower than that from Vero and Namalwa cells. This study provide new host cells for pro-UK production.