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Damage to non–dicamba resistant (non-DR) soybean [Glycine max (L.) Merr.] has been frequent in geographies where dicamba-resistant (DR) soybean and cotton (Gossypium hirsutum L.) have been grown and sprayed with the herbicide in recent years. Off-target movement field trials were conducted in northwest Arkansas to determine the relationship between dicamba concentration in the air and the extent of symptomology on non-DR soybean. Additionally, the frequency and concentration of dicamba in air samples at two locations in eastern Arkansas and environmental conditions that impacted the detection of the herbicide in air samples were evaluated. Treatment applications included dicamba at 560 g ae ha−1 (1X rate), glyphosate at 860 g ae ha−1, and particle drift retardant at 1% v/v applied to 0.37-ha fields with varying degrees of vegetation. The relationship between dicamba concentration in air samples and non-DR soybean response to the herbicide was more predictive with visible injury (generalized R2 = 0.82) than height reduction (generalized R2 = 0.43). The predicted dicamba air concentration resulting in 10% injury to soybean was 1.60 ng m−3 d−1 for a single exposure. The predicted concentration from a single exposure to dicamba resulting in a 10% height reduction was 3.78 ng m−3 d−1. Dicamba was frequently detected in eastern Arkansas, and daily detections above 1.60 ng m−3 occurred 17 times in the period sampled. The maximum concentration of dicamba recorded was 7.96 ng m−3 d−1, while dicamba concentrations at Marianna and Keiser, AR, were ≥1 ng m−3 d−1 in six samples collected in 2020 and 22 samples in 2021. Dicamba was detected consistently in air samples collected, indicating high usage in the region and the potential for soybean damage over an extended period. More research is needed to quantify the plant absorption rate of volatile dicamba and to evaluate the impact of multiple exposures of gaseous dicamba on non-targeted plant species.

The threat of herbicide-resistant weed species, such as Palmer amaranth, has driven the development of robust weed management programs that rely on more than chemicals for weed control. Previous research has shown that zero-tolerance weed thresholds, cover crops, deep tillage, and diverse herbicide programs are effective strategies for controlling Palmer amaranth. Unfortunately, research investigating the integration of all four of these weed management strategies in a system is lacking. To better leverage these integrated weed management strategies in cotton production systems, a long-term study was initiated in fall 2018 near Marianna, AR, with zero tolerance, deep tillage, a cereal rye cover crop, and either a dicamba or non-dicamba in-crop herbicide program as factors. Results found that total Palmer amaranth emergence was reduced 76% as the result of deep tillage in 2019 and, in the absence of a zero-tolerance strategy, 73% in 2020. In the absence of a zero-tolerance strategy, the combination of a non–cover crop strategy and dicamba herbicide program decreased total Palmer amaranth emergence by 73%, while the combination of a cover crop strategy and dicamba herbicide program decreased total Palmer amaranth emergence by 78% compared to the combination of a cover crop and non-dicamba herbicide program. Under a zero-tolerance strategy in 2019, tillage reduced cotton yield by 12% and partial returns by US$370 ha−1. In 2020, tillage reduced cotton yield by 14% and partial returns of US$371 ha−1 under a non-zero-tolerance strategy, while a 12% yield reduction and a US$260 ha−1 decrease in partial returns were observed under a zero-tolerance strategy. In 2019, the non-dicamba program resulted in greater partial returns than the dicamba in-crop program because of greater yield and lower program costs. However, in 2020, partial returns were greater for the dicamba in-crop herbicide program owing to greater yields achieved by this program.

Glufosinate resistance in Palmer amaranth (Amaranthus palmeri S. Watson) was recently detected in three accessions from Arkansas, USA. Amaranthus palmeri is the first and only broadleaf weed species resistant to this herbicide, and the resistance mechanism is still unclear. A previous study characterized the glufosinate resistance level in the accessions from Arkansas. A highly glufosinate-resistant accession was further used to investigate the mechanism conferring glufosinate resistance in A. palmeri. Experiments were designed to sequence the herbicide target enzyme cytosolic and chloroplastic glutamine synthetase isoforms (GS1 and GS2, respectively) and quantify copy number and expression. Absorption, translocation, and metabolism of glufosinate using the 14C-labeled herbicide were also evaluated in the resistant and susceptible accessions. The glufosinate-resistant accession had an increase in copy number and expression of GS2 compared with susceptible plants. All accessions showed only one GS1 copy and no differences in expression. No mutations were identified in GS1 or GS2. Absorption (54% to 60%) and metabolism (13% to 21%) were not different between the glufosinate-resistant and glufosinate-susceptible accessions. Most residues of glufosinate (94% to 98%) were present in the treated leaf. Glufosinate translocation to tissues above the treated leaf and in the roots was not different among accessions. However, glufosinate translocation to tissues below the treated leaf (not including roots) was greater in the resistant A. palmeri (2%) compared with the susceptible (less than 1%) accessions. The findings of this paper strongly indicate that gene amplification and increased expression of the chloroplastic glutamine synthetase enzyme are the mechanisms conferring glufosinate resistance in the A. palmeri accession investigated. Thus far, no additional resistance mechanism was observed, but further investigations are ongoing.

Field experiments were conducted across multiple sites in 2012 and 2013 to describe sensitivity of soybean to 2,4-D (six doses) and dicamba (seven doses) at V3 and R1 growth stages. Further experiments were conducted under greenhouse conditions in 2017 and 2018 to compare soybean response to several dicamba herbicides across a broader range of doses than those tested in the field.Soybean yield loss was 6.1-fold greater from 2,4-D exposure at V3 compared to R1 and 1.4 times greater from dicamba exposure at R1 than at V3. In V3 exposures, soybean was 15.4 times more sensitive to dicamba than 2,4-D and 134.4-fold more sensitive to dicamba when exposed at R1. Plant injury and height correlations to grain yield resulted in coefficients ranging from 0.65 to 0.91. In greenhouse experiments, five dicamba products were tested at up to 19 doses and as low as 0.002 g ae haThese data suggest that the sensitivity of soybean to dicamba is much greater than what has previously been reported. However, as has been indicated by previous work, that injury does not always result in yield loss. © 2022 Society of Chemical Industry.

Palmer amaranth has developed resistance to at least seven herbicide sites of action in the Cotton Belt of the United States, leaving producers with fewer options to manage this weed. Previous research with corn and newly commercially released soybean systems have found the use of 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibiting herbicides such as isoxaflutole (IFT) to be effective at managing Palmer amaranth. Consequently, a new transgenic cultivar of cotton is being developed with tolerance to IFT, allowing for in-crop applications of the herbicide. Two separate studies were conducted near Marianna, AR, in 2019 and replicated in 2020, to investigate the crop safety and utility of IFT when added to cotton herbicide programs. Herbicide programs featured IFT as a preemergence or early-postemergence option, residual herbicides in subsequent postemergence applications, and the presence or absence of a layby application. The use of IFT did not significantly impact cotton injury or yield, whereas the use of layered residual herbicides, including IFT, increased Palmer amaranth control compared to those without. Regardless of earlier use of IFT, layby applications were needed for season-long control of Palmer amaranth, entireleaf morningglory, broadleaf signalgrass, and johnsongrass, as evidenced by greater than a 20 percentage point improvement in control of all weeds when a layby application was made. Overall, findings from these studies indicate IFT to be a suitable tool for managing Palmer amaranth and will provide an additional site of action for cotton herbicide programs. Sequential herbicide applications and overlaying residuals were found to be paramount for managing Palmer amaranth throughout the season.

Dicamba residues in sprayers are difficult to remove and may interact with subsequent herbicides, including contact herbicides labeled for use in soybean. Without proper tank cleanout, applicators treating dicamba-resistant and non–dicamba resistant crops are at risk of contaminating the spray solution with dicamba residue from previous applications. Experiments were conducted in Fayetteville, AR, in 2018 and 2019, with the first experiment evaluating consequences of dicamba tank contamination with contact herbicides and the second experiment addressing the impact of dicamba exposure on a glufosinate-resistant soybean cultivar relative to a contact herbicide application. Experiments for tank contamination and timing of dicamba exposure were designed as a three-factor and a two-factor randomized complete block with four replications, respectively, considering site-year as a fixed effect in each experiment. Dicamba at 0, 0.056, 0.56, and 5.6 g ae ha−1 was applied alone, with glufosinate, with acifluorfen, or with glufosinate plus acifluorfen to V3 soybean. Dicamba applied in combination with contact herbicides exacerbated visible auxin symptomology over dicamba alone at 21 and 28 d after treatment (DAT), while dicamba at 5.6 g ae ha−1 reduced soybean height. Injury and height reductions caused by dicamba mixtures with contact herbicides did not reduce grain yield. In the second experiment, dicamba was applied at 2.8 g ae ha−1 at VC, V1, V2, and V3 and at 3, 7, and 10 d after a glufosinate application to V3 soybean (DATV3). Greater soybean injury was observed when dicamba exposure followed a glufosinate application than when dicamba preceded glufosinate or was applied in a mixture with glufosinate, with yield reductions resulting from 7 and 10 DATV3 dicamba applications. Dicamba exposure in the presence of contact herbicides resulted in increased auxin symptomology and can be intensified if soybean are exposed to dicamba following a contact herbicide application.

This study was undertaken to investigate the nutrient composition and sensory properties of wheat flour bread substituted with defatted and undefatted cashew kernel flours. Cashew kernel was processed into flour and thereafter divided into two portions. One portion was left undefatted while the other portion was defatted using a hydraulic press. Bread was prepared from the blends of wheat and defatted/undefatted cashew kernel flours using 90:10, 80:20, 70:30, of wheat flour to defatted cashew kernel flour (DCF) and wheat flour to undefatted cashew kernel flour (UCF), and 100% wheat flour as control. Bread samples were subjected to sensory evaluation within 30 minutes of production. Proximate analysis and amino acid profile of the bread samples were carried out using standard methods. Result of the proximate analysis of the bread samples revealed a significant (p

A thorough understanding of commonly used herbicide application practices and technologies is needed to provide recommendations and determine necessary application education efforts. An online survey to assess ground and aerial herbicide application practices in Arkansas was made available online in spring 2019. The survey was direct-emailed to 272 agricultural aviators and 831 certified commercial pesticide applicators, as well as made publicly available online through multiple media sources. A total of 124 responses were received, of which 75 responses were specific to herbicide applications in Arkansas agronomic crops, accounting for approximately 49% of Arkansas’ planted agronomic crop hectares in 2019. Ground and aerial application equipment were used for 49% and 51% of the herbicide applications on reported hectares, respectively. Rate controllers were commonly used application technologies for both ground and aerial application equipment. In contrast, global positioning system-driven automatic nozzle and boom shut-offs were much more common on ground spray equipment than aerial equipment. Applicator knowledge of nozzles and usage was limited, regardless of ground or aerial applicators, as only 28% of respondents provided a specific nozzle type used, indicating a need for educational efforts on nozzles and their importance in herbicide applications. Of the reported nozzle types, venturi nozzles and straight-stream nozzles were the most commonly used for ground and aerial spray equipment, respectively. Spray carrier volumes of 96.3 and 118.8 L ha−1 for ground spray equipment and 49.6 and 59.9 L ha−1 for aerial application equipment were the means of reported spray volumes for systemic and contact herbicides, respectively. Respondents indicated application optimization was a major benefit of utilizing newer application technologies, herbicide drift was a primary challenge, and research needs expressed by respondents included adjuvants, spray volume efficacy, and herbicide drift. Findings from this survey provided insight into current practices, technologies, and needs of Arkansas herbicide applicators. Research and education efforts can be implemented as a result to address aforementioned needs while providing applied research-based information to applicators based on current practices.

The commercialization of dicamba-resistant soybean has resulted in increased concern for off-target movement of dicamba onto sensitive vegetation. To mitigate the off-target movement through physical drift, one might consider use of rope wicks and other wiper applicators. Although wiper-type application methods have been efficacious in pasture settings, the utility of dicamba using wiper applicators in agronomic crops is not available in scientific literature. To determine the utility of roller wipers for dicamba applications in dicamba-resistant soybean, two separate experiments were conducted in the summer of 2020 and replicated in both Keiser and Fayetteville, AR, USA.Utilizing opposing application directions and a 2:1:1 ratio of water: formulated glyphosate: formulated dicamba were the most efficacious practices for controlling Palmer amaranth. The high herbicide concentrations and wiping in opposing directions increased dicamba-resistant soybean injury when the wiper contacted the crop, but no yield loss was observed because of this injury. Broadcast applications resulted in greater Palmer amaranth mortality than roller wiper applications, and the most effective roller wiper treatments were when two sequential applications were made inside the crop canopy.Dicamba applications require adequate coverage for optimum weed control. While efforts can be made to increase roller wiper efficacy by optimizing coverage and timing of applications, broadcast applications are superior to roller wiper applicators for weed control. Roller wiper applications did not reduce soybean yield, thus wiper-type applications may be safely used in dicamba-resistant soybean, albeit the likelihood for off-target damage caused by volatilization of these treatments would need to be investigated. © 2022 The Authors. Pest Management Science published by John WileySons Ltd on behalf of Society of Chemical Industry.

Expansion of dicamba-resistant crops increased the frequency of off-target movement issues, especially in the midsouthern United States. Six field trials were conducted over two growing seasons with the purpose to determine the contribution of volatilization and physical suspension of particles to the off-target movement of dicamba when applied with glyphosate and imazethapyr - a non-volatile herbicide used as a tracer for physical off-target movement. Applications included dicamba at 560 g ha

Field experiments were conducted in 2012 and 2013 across four locations for a total of 6 site-years in the midsouthern United States to determine the effect of growth stage at exposure on soybean sensitivity to sublethal rates of dicamba (8.8 g ae ha−1) and 2,4-D (140 g ae ha−1). Regression analysis revealed that soybean was most susceptible to injury from 2,4-D when exposed between 413 and 1,391 accumulated growing degree days (GDD) from planting, approximately between V1 and R2 growth stages. In terms of terminal plant height, soybean was most susceptible to 2,4-D between 448 and 1,719 GDD, or from V1 to R4. However, maximum susceptibility to 2,4-D was only between 624 and 1,001 GDD or from V3 to V5 for yield loss. As expected, soybean was sensitive to dicamba for longer spans of time, ranging from 0 to 1,162 GDD for visible injury or from emergence to R2. Likewise, soybean height was most affected when dicamba exposure occurred between 847 and 1,276 GDD or from V4 to R2. Regarding grain yield, soybean was most susceptible to dicamba between 820 and 1,339 GDD or from V4 to R2. Consequently, these data indicate that soybean response to 2,4-D and dicamba can be variable within vegetative or reproductive growth stages; therefore, specific growth stage at the time of exposure should be considered when evaluating injury from off-target movement. In addition, application of dicamba near susceptible soybean within the V4 to R2 growth stages should be avoided because this is the time of maximum susceptibility. Research regarding soybean sensitivity to 2,4-D and dicamba should focus on multiple exposure times and also avoid generalizing growth stages to vegetative or reproductive.

Protoporphyrinogen oxidase- (PPO-) resistant Amaranthus palmeri (S.) Wats. (Palmer amaranth) was confirmed in Arkansas in 2015. Field trials were conducted in Crawfordsville, Gregory, and Marion, Arkansas in 2016, and Crawfordsville and Marion in 2017, assessing PPO-resistant Palmer amaranth control options in Glycine max (L.) Merr. (soybean). Twelve trials consisted of 26 preemergence (PRE) treatments, evaluated for Palmer amaranth control and density reduction at 28 days after treatment (DAT). Treatments that consisted of PPO- or acetolactate synthase- (ALS-) inhibiting herbicides such as flumioxazin (72 g ai ha−1) or sulfentrazone + cloransulam (195 g ha−1 + 25 g ha−1) controlled Palmer amaranth

Managing emerged weeds that have evolved resistance to acetyl CoA carboxylase (ACCase)-inhibiting herbicides is a challenging task. A dose-response experiment was conducted on barnyardgrass biotypes resistant (R) and susceptible (S) to three aryloxyphenoxypropionate herbicides cyhalofop-butyl (CyB), fenoxaprop-ethyl (FeE), and quizalofop-ethyl (QuE) along with investigations into the potential resistance mechanism of these biotypes. The tested R barnyardgrass biotypes had strong resistance to CyB and FeE (resistant/susceptible ratio: 7.9-14.4) but weak resistance to QuE (resistant/susceptible ratio: 2.4-3.1). Absorption, translocation, and total metabolism of CyB and QuE were not associated with differences among S and R barnyardgrass biotypes. However, differences between S and R barnyardgrass were observed in production of active acid forms of each herbicide (cyhalofop-acid and quizalofop-acid). Production of cyhalofop-acid was1.6-fold less in R barnyardgrass (3-8%) for 24 h after herbicide application than in the S barnyardgrass (8-16%). Meanwhile, production of quizalofop-acid was less in R barnyardgrass (14%) throughout the study period than in the S barnyardgrass (22%). Sequencing results of ACCase gene showed no difference between S and R barnyardgrass. Overall results show that a non-target-site resistance mechanism altering metabolism of CyB and QuE likely contributes to resistance of the barnyardgrass biotypes to these herbicides.

This study is part of the Children&rsquo, s Healthy Living program in U.S. Affiliated Pacific region. The objectives were to estimate overweight and obesity (OWOB) prevalence and identify possible related risk factors among ethnic groups in Guam. In 2013, 865 children (2&ndash, 8 years) were recruited via community-based sampling from select communities in Guam. Children&rsquo, s demographic and health behavior information, dietary intake, and anthropometric measurements were collected. Logistic regression, odds ratio, t-tests, and chi-square tests were used to determine differences and assess covariates of OWOB. The results indicate that 58% of children were living below the poverty level, 80% were receiving food assistance, and 51% experienced food insecurity. The majority of children surveyed did not meet recommendations for: sleep duration (59.6%), sedentary screen-time (83.11%), or fruit (58.7%) and vegetable (99.1%) intake, and consumed sugar sweetened beverages (SSB) (73.7%). OWOB affected 27.4% of children. Children affected by OWOB in this study were statistically more likely (p = 0.042) to suffer from sleep disturbances (p = 0.042) and consume marginally higher amounts (p value = 0.07) of SSB compared to children with healthy weight. Among Other Micronesians, children from families who considered themselves &lsquo, integrated&rsquo, into the culture were 2.05 (CI 0.81&ndash, 5.20) times more likely to be affected by OWOB. In conclusion, the OWOB prevalence among 2&ndash, 8-year-olds in Guam was 27.4%, and compared with healthy weight children, children with OWOB were more likely to have educated caregivers and consume more SSBs. Results provide a basis for health promotion and obesity prevention guidance for children in Guam.

In an effort to characterize food costs in the United States (US)-affiliated Pacific Region, a first-time food cost survey was conducted in March 2014. A market basket survey was developed using an adaptation of the US Department of Agriculture Thrifty Food Plan. Surveys were conducted in the states of Alaska and Hawai‘i; Portland, Oregon; the US-affiliated Pacific Islands of American Samoa (American Samoa); Commonwealth of the Northern Mariana Islands; the island of Pohnpei within the Federated States of Micronesia; Guam; Republic of the Marshall Islands; and Republic of Palau. Urban and rural communities were included. Multiple stores in multiple communities were surveyed in each jurisdiction. Food retailers (N = 74) ranged from convenience markets to supermarkets. Not all foods in the market basket survey were available in each of the communities. Inspection of available income data also showed that food costs represented a higher percentage of household income for American Samoa than those of Alaska, Hawai‘i, and Portland. Thrifty Food Plan weighted weekly totals for the region ranged from $181.90 to $264.30. Weighting was based on the amount of the item converted to grams required for the Thrifty Food Plan menu. These food costs are significantly higher than those of Portland ($142.00) for the survey period. Protein foods, grains, vegetables, fruit, and dairy were the 5 most costly components, in descending order. Food affordability was assessed by comparing food costs across jurisdictions and examining estimated food costs to reported average jurisdiction incomes. The survey is intended to help inform public health policy and educational programs in the region. A locally adapted food survey would benefit future analyses, regional policy, and educational efforts.

Dose-response experiments were conducted to assess the sensitivity of one susceptible and three putative resistant (R1, R2, and R3) barnyardgrass [Echinochloa crus-galli (L.) P. Beauv.] biotypes to florpyrauxifen-benzyl and cyhalofop-butyl alone and as a formulated premix. Subsequently, potential resistance mechanisms of the barnyardgrass were evaluated. Based on biomass reduction results, resistant/susceptible ratios were calculated for R1 (7.0–50), R2 (7.0–150), and R3 (18–214) biotypes. Absorption and translocation of [14C]-florpyrauxifen-benzyl decreased in R1 and R3 biotypes, but not for [14C]-cyhalofop-butyl. The metabolism of [14C]-florpyrauxifen-benzyl to [14C]-florpyrauxifen-acid was >2-fold less in resistant biotypes (9–11%) than in the susceptible biotype (23%). Moreover, the production of [14C]-florpyrauxifen-acid in susceptible barnyardgrass (not in the R biotypes) increased 3-fold when florpyrauxifen-benzyl and cyhalofop-butyl were applied in mixture compared to florpyrauxifen-benzyl applied alone. The tested barnyardgrass biotypes had no mutation in the Transport Inhibitor Response1, auxin-signaling F-box, and acetyl coenzyme A carboxylase genes. Although further studies on cyhalofop-butyl resistance with respect to analysis of specific metabolites are needed, our findings in this study demonstrates that the evolution of florpyrauxifen-benzyl resistance in multiple resistant barnyardgrass can be related to non-target-site resistance mechanisms reducing absorption and translocation of the herbicide and causing reduced conversion or rapid degradation of florpyrauxifen-acid.

Herbicide resistance may evolve at a rapid rate with the lack of proper adoption of best management practices (BMPs). This wide-spread resistance problem is particularly impactful for fields with problematic weeds such as Palmer amaranth (Amaranthus palmeri). Protoporphyrinogen oxidase (PPO)-inhibiting herbicides have been one of the remaining effective sites of action (SOAs) for the control of Palmer amaranth, but even these herbicides have begun to fail in many soybean fields across Arkansas, USA. The objective of this research was to determine which of the most commonly used PPO-inhibiting herbicides have the greatest effect on two putative PPO-resistant Palmer amaranth populations, compared to a susceptible standard, when applied preemergence (PRE) and postemergence (POST); as well as to test for multiple resistance to other commonly used herbicides. A dose-response study was conducted under greenhouse conditions that examined five PRE herbicides and four POST herbicides on one PPO-susceptible and two PPO-resistant populations. Complete control was not achieved at the 8X rate with any PPO-inhibiting herbicide at the PRE application; whereas for the POST application, complete control was not achieved until after the 32X rate for all herbicides. Furthermore, twenty-one different herbicides, representing various SOAs, were used to test various application timings (preplant incorporated (PPI), PRE and POST) on these populations at a 1X field rate. Both of the PPI herbicides (trifluralin and pendimethalin) provided 9.7% mortality of Palmer amaranth. Only two PRE herbicides (in 2 SOAs) showed greater than 85% mortality and three POST application herbicides (3 SOAs) all had greater than 95% mortality of both Palmer amaranth populations. Thus, it is likely that PPO-inhibiting herbicides or the other commonly used herbicides, which were tested, cannot be solely relied upon in the field. Thus, the use of multiple effective sites of action along with other integrated weed management tactics need to be a focus for management of this species.

Emotional contagion, a basic component of empathy defined as emotional state-matching between individuals, has previously been shown in dogs even upon solely hearing negative emotional sounds of humans or conspecifics. The current investigation further sheds light on this phenomenon by directly contrasting emotional sounds of both species (humans and dogs) as well as opposed valences (positive and negative) to gain insights into intra- and interspecies empathy as well as differences between positively and negatively valenced sounds. Different types of sounds were played back to measure the influence of three dimensions on the dogs' behavioural response. We found that dogs behaved differently after hearing non-emotional sounds of their environment compared to emotional sounds of humans and conspecifics ("Emotionality" dimension), but the subjects responded similarly to human and conspecific sounds ("Species" dimension). However, dogs expressed more freezing behaviour after conspecific sounds, independent of the valence. Comparing positively with negatively valenced sounds of both species ("Valence" dimension), we found that, independent of the species from which the sound originated, dogs expressed more behavioural indicators for arousal and negatively valenced states after hearing negative emotional sounds. This response pattern indicates emotional state-matching or emotional contagion for negative sounds of humans and conspecifics. It furthermore indicates that dogs recognized the different valences of the emotional sounds, which is a promising finding for future studies on empathy for positive emotional states in dogs.

With the evolution of glyphosate-resistant weeds, cotton producers now rely on multiple applications of soil-residual herbicides tank-mixed with postemergence (POST) herbicides. However, the current soil-residual herbicides represent a relatively low number of herbicide mechanisms of action (MOAs), with the same few MOAs being utilized year after year. Therefore, it is important to introduce new MOAs to slow the likelihood of further resistance development. A field experiment was conducted in 2012 and 2013 to determine if fluridone, a bleaching herbicide currently not used in any agronomic cropping system, applied preemergence (PRE) would provide effective season-long control of Palmer amaranth as well as reduce the number of POST applications throughout the season. Fluridone PRE at 224, 336, and 448 g ai ha-1 did not eliminate the need for POST herbicides for Palmer amaranth control in cotton in either year. Fluridone, regardless of rate, provided comparable control to fluometuron in 2012; however in 2013, fluometuron provided less control than fluridone. Although moderate season-long control was observed in 2013, greater seed cotton yields were obtained in 2013 than in 2012, which is likely a result of greater control during the critical period for weed control. Based on this experiment, fluridone will not provide effective season-long Palmer amaranth control in the absence of a multiple POST-herbicide program.

The herbicide fluridone is a soil-residual herbicide that should provide effective control of several problematic agronomic weeds, but because of herbicide persistence, injury to rotational crops is possible. In this experiment, multiple rates of fluridone were applied PRE to cotton at four irrigated locations across Arkansas to determine the risk of fluridone persisting and injuring subsequently planted wheat, corn, soybean, rice, grain sorghum, and sunflower. The multiple rates of fluridone were compared with fluometuron and evaluated for percentage of crop injury, crop density, and potential yield loss for each crop at the end of the subsequent growing season. Regardless of the location, wheat exhibited the greatest injury with 13 to 26% at Fayetteville (silt loam), 8 to 15% at Pine Tree (silt loam), 2 to 7% at Keiser (silty clay), and 3 to 8% at Rohwer (silty clay). Along with high levels of injury to wheat, fluridone at 900 g ai ha−1caused loss of wheat stands to 29 plants m−1row compared with fluometuron, which had stands of 49 plants m−1row. Although injury occurred in wheat at all locations, no rate of fluridone reduced wheat yields compared with fluometuron. Injury to grain sorghum ranged from 5 to 10% from all rates of fluridone at Pine Tree. Fluridone at 900 g ha−1(11 plants m−1row) also reduced grain sorghum stands at Pine Tree over that of fluometuron (19 plants m−1row). A decrease in grain sorghum yield was also observed from fluridone at 448, 673, and 900 g ha−1compared with fluometuron at Pine Tree. At Keiser, rice exhibited significant levels of injury (1 to 13%) from fluridone 393 d after treatment. In conclusion, injury to a wheat rotational crop is more likely following an application of fluridone in cotton than is injury to other rotational crops, but yield reductions are not expected for most rotational crops when fluridone is applied to cotton at an anticipated labeled rate of 224 g ha−1.

Glyphosate-resistant Palmer amaranth is considered the most troublesome weed in agronomic crops in the Midsouth. The reliance on multiple herbicide mechanisms of action (MOA) and soil-residual herbicides has increased over the past several years due to the ever growing issue of herbicide-resistant weeds. Field experiments were conducted at several locations in Arkansas to determine the efficacy of fluridone on Palmer amaranth in glyphosate-resistant and glufosinate-resistant cotton herbicide programs and to determine the length of residual fluridone activity when applied preemergence (PRE). Fluridone has a unique MOA and is currently not registered as a stand-alone herbicide for use in cotton. In the length of residual experiment, when rainfall was adequate, fluridone applied PRE at rates greater than 224 g a.i. ha-1 provided > 90% Palmer amaranth control for 6 wk after application; however, effective season-long Palmer amaranth control was not achieved with any rate of fluridone alone. Fluridone alone applied 14-d preplant or PRE did not provide greater Palmer amaranth control than a standard herbicide application. When fluridone was integrated into a glufosinate-based herbicide program, PRE-applied fluridone at 224, 336, and 448 g ha-1 did not provide greater Palmer amaranth control than the standard herbicide program that included fluometuron. Based on these experiments, fluridone should not be applied as a stand-alone herbicide in cotton, nor will it reduce the number of POST applications needed for effective Palmer amaranth control in glufosinate-resistant cotton.

Field studies were conducted in Alabama, Arkansas, Georgia, Louisiana, Mississippi, North Carolina, and Tennessee during 2010 and 2011 to determine the effect of glufosinate application rate on LibertyLink and WideStrike cotton. Glufosinate was applied in a single application (three-leaf cotton) or sequential application (three-leaf followed by eight-leaf cotton) at 0.6, 1.2, 1.8, and 2.4 kg ai ha−1. Glufosinate application rate did not affect visual injury or growth parameters measured in LibertyLink cotton. No differences in LibertyLink cotton yield were observed because of glufosinate application rate; however, LibertyLink cotton treated with glufosinate yielded slightly more cotton than the nontreated check. Visual estimates of injury to WideStrike cotton increased with each increase in glufosinate application rate. However, the injury was transient, and by 28 d after the eight-leaf application, no differences in injury were observed. WideStrike cotton growth was adversely affected during the growing season following glufosinate application at rates of 1.2 kg ha−1 and greater; however, cotton height and total nodes were unaffected by glufosinate application rate at the end of the season. WideStrike cotton maturity was delayed, and yields were reduced following glufosinate application at rates of 1.2 kg ha−1 and above. Fiber quality of LibertyLink and WideStrike cotton was unaffected by glufosinate application rate. These data indicate that glufosinate may be applied to WideStrike cotton at rates of 0.6 kg ha−1 without inhibiting cotton growth, development, or yield. Given the lack of injury or yield reduction following glufosinate application to LibertyLink cotton, these cultivars possess robust resistance to glufosinate. Growers are urged to be cautious when increasing glufosinate application rates to increase control of glyphosate-resistant Palmer amaranth in WideStrike cotton. However, glufosinate application rates may be increased to maximum labeled rates when making applications to LibertyLink cotton without fear of reducing cotton growth, development, or yield.

SummaryThe question of whether animals have emotions and respond to the emotional expressions of others has become a focus of research in the last decade [1–9]. However, to date, no study has convincingly shown that animals discriminate between emotional expressions of heterospecifics, excluding the possibility that they respond to simple cues. Here, we show that dogs use the emotion of a heterospecific as a discriminative cue. After learning to discriminate between happy and angry human faces in 15 picture pairs, whereby for one group only the upper halves of the faces were shown and for the other group only the lower halves of the faces were shown, dogs were tested with four types of probe trials: (1) the same half of the faces as in the training but of novel faces, (2) the other half of the faces used in training, (3) the other half of novel faces, and (4) the left half of the faces used in training. We found that dogs for which the happy faces were rewarded learned the discrimination more quickly than dogs for which the angry faces were rewarded. This would be predicted if the dogs recognized an angry face as an aversive stimulus. Furthermore, the dogs performed significantly above chance level in all four probe conditions and thus transferred the training contingency to novel stimuli that shared with the training set only the emotional expression as a distinguishing feature. We conclude that the dogs used their memories of real emotional human faces to accomplish the discrimination task.

Almost 40% of children are overweight or obese by age 8 years in the US-Affiliated Pacific, inclusive of the five jurisdictions of Alaska, Hawaii, American Samoa, Guam, and the Commonwealth of the Northern Mariana Islands. This article describes how the Children's Healthy Living (CHL) Program used the ANGELO (Analysis Grid for Environments/Elements Linked to Obesity) model to design a regional intervention to increase fruit and vegetable intake, water consumption, physical activity, and sleep duration and decrease recreational screen time and sugar-sweetened beverage consumption in young children ages 2-8 years.Using the ANGELO model, CHL (1) engaged community to identify preferred intervention strategies, (2) reviewed scientific literature, (3) merged findings from community and literature, and (4) formulated the regional intervention.More than 900 community members across the Pacific helped identify intervention strategies on importance and feasibility. Nine common intervention strategies emerged. Participants supported the idea of a regional intervention while noting that cultural and resource differences would require flexibility in its implementation in the five jurisdictions. Community findings were merged with the effective obesity-reducing strategies identified in the literature, resulting in a regional intervention with four cross-cutting functions: (1) initiate or strengthen school wellness policies; (2) partner and advocate for environmental change; (3) promote CHL messages; and (4) train trainers to promote CHL behavioral objectives for children ages 2-8 years. These broad functions guided intervention activities and allowed communities to tailor activities to maximize intervention fit.Using the ANGELO model assured that the regional intervention was evidence based while recognizing jurisdiction context, which should increase effectiveness and sustainability.

A standardized experiment was conducted during 2009 and 2010 at 20 location-years across U.S. cotton (Gossypium hirsutum L.)-producing states to compare the N use requirement of contemporary cotton cultivars based on their planting seed size. Treatments consisted of three cotton varieties with planting seed of different numbers of seed per kg and N rates of 0, 45, 90, and 134 kg ha–¹. Soil at each trial location was sampled and tested for nitrate presence. High levels of soil nitrate (>91 N-NO₃– kg ha–¹) were found in Arizona and western Texas, and soil nitrate in the range of 45 to 73 kg N-NO₃– ha–¹ was found at locations in the central United States. Cotton lint yield responded to applied N at 11 of 20 locations. Considering only sites that responded to applied N, highest lint yields were achieved with 112 to 224 kg ha–¹of applied plus pre-plant residual soil NO₃—translating to an optimal N requirement of 23 kg ha–¹ per 218 kg bale of lint produced. Among the varieties tested those with medium-sized seed produced higher yields in response to N than did larger and smaller seeded varieties. Varieties with larger seed had longer and stronger fibers, higher fiber length uniformity than small seeded varieties and decreased micronaire. Seed protein and oil increased and decreased slightly in response to increasing amounts of soil nitrate plus applied N, respectively.

Canine histiocytic sarcoma (HS) is an aggressive neoplasm that is generally associated with a poor prognosis. CCNU is considered first-line medical therapy, although the majority of dogs ultimately develop progressive disease. The objective of this study was to evaluate the efficacy of dacarbazine as a rescue agent for HS.Medical records of dogs diagnosed with HS that received at least one dose of dacarbazine were reviewed. Information collected and analyzed included signalment, disease distribution, treatment history, dacarbazine treatments (including dose, interval and total number of cycles), adverse events, and response to treatment.Seventeen dogs were included, all of which had disseminated or metastatic disease and had received prior treatment with CCNU. Three dogs achieved partial remission for an overall response rate of 17.6%. The overall median event-free survival (EFS) was 21 days. For dogs that experienced an objective response, the EFS was 70 days. Toxicity secondary to dacarbazine was generally mild and self-limiting.In the setting of advanced disease, dacarbazine appears to have modest activity against HS and warrants further investigation.

The biological target is becoming increasingly more important in pesticide spray evaluation and to research directed toward improving the safety and effectiveness of pesticides. Various techniques have been used to study the nature of drops which impact and deposit on tree foliage. A few basic techniques are summarized in this paper. Additionally, results are presented on the assessment of drops directly on foliage of slash pine (Pinus elliottii Engelm.) and Ocala sand pine (Pinus clausa [Chapm. ex Engelm.] Vasey ex Sarg. var. clausa). This was one of several techniques used to assess spray coverage from an aerial spray on a project conducted by the United States Department of Agriculture, Forest Service, in cooperation with the Florida Forest Service. Testing was conducted at the Withlacoochee State Seed Orchard, Florida, during February 1980. Results show a greater deposit of spray drops in the upper tree crown than in the lower crown. This was true for both pine species; however, fewer drops per linear length of needle were found on the Ocala sand pine when compared to the slash pine. Zusammenfassung Zur Tropfchenverteilung auf Kiefernnadeln bei aviochemischen Bekampfungsmasnahmen Im Hinblick auf die immer wichtiger werdenden biologischen Aspekte sowie die Erzielung noch groerer Sicherheit und Wirksamkeit bei der Ausbringung pestizider Spruhmittel wird ein Literaturuberblick uber verschiedene Spruhtechniken und ihre Ergebnisse gegeben. Zusatzlich werden die Erfahrungen aus eigenen Versuchen, die in den USA an Pinus elliottii und P. clausa im Februar 1960 durchgefuhrt wurden, mitgeteilt. Es ergab sich bei beiden Kiefernarten eine grosere Ablagemenge an Tropfchen in der oberen Kronenregion, jedoch waren die Nadeln von Pinus clausa pro Langeneinheit mit einer groseren Zahl Tropfchen belegt als jene von P. elliottii.

A catalogue of pottery, all or most of it from the 1911 excavations of the British School at Phylakopi, set out by shape and motif, is accompanied by a summary collation of evidence for dating and provenance across the Cyclades. Investigation of the material provides some new information about the character of the Phylakopi pottery and the contexts in which it was found. The material prompts observations on a number of questions in the area of Cycladic prehistoric ceramics, prominent among which is the transition from E to MC Some suggestions are offered as to topics which require investigation. The study should be seen as a continuation of work by the same writer which appeared in BSA 69 (1974).Ένας κατάλογος αγγείων και οστράκων, όλα ή τα περισσότερα από αυτά από τις ανασκαφές της Βρεταννικής Σχολής στη Φυλακωπή κατά το 1911, οργανωμένος κατά σχήματα και μοτίβα, συνοδεύεται από μία σύντομη επισκόπηση των ενδείξεων που υπάρχουν από τα νησιά των Κυκλάδων γενικά σχετικά με τη χρονολόγηση και την προέλευσή τους. Από τη μελέτη του υλικού προκύπτουν ορισμένα νέα στοιχεία για το χαρακτήρα της ντόπιας κεραμεικής της Φυλακωπής και τις ανασκαφικές της συνάφειες. Το άρθρο συμπεριλαμβάνει παρατηρήσεις πάνω σε διάφορα θέματα που αφορούν στην προϊστορική κεραμεική των Κυκλάδων – το πιό σημαντικό από τα οποία αναφέρεται στο θέμα της μετάβασης από την Πρωτοκυκλαδική στη Μεσοκυκλαδική περίοδο. Επιπλέον, διατυπώνονται προτάσεις για θέμαια που χρήζουν περαιτέρω έρευνας. Ｈ παρούσα μελέτη θα πρέπει να θεωρηθεί ως συνέχεια της δημοσίευσης του συγγραφέα στο BSA 69 (1974).