1. Extra virgin olive oil improved body weight and insulin sensitivity in high fat diet-induced obese LDLr−/−.Leiden mice without attenuation of steatohepatitis
- Author
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Manuel Romero-Gómez, Lourdes M. Varela, Anabel Rojas, Amparo Luque Sierra, Alejandro Martin-Montalvo, Javier López-Beas, Abdelkrim Hmadcha, Eloísa Andújar, Leticia Álvarez-Amor, Franz Martín, Genoveva Berná, Mónica Pérez-Alegre, Antonio Cárdenas, Robert Kleemann, Rocío Gallego-Durán, Lucía López-Bermudo, Mª José Robles-Frías, Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Junta de Andalucía, Instituto de Salud Carlos III, European Commission, [Álvarez-Amor,L, Sierra,AL, Cárdenas,A, López-Bermudo,L, López-Beas,J, Andújar,E, Pérez-Alegre,M, Varela,LM, Martin-Montalvo,A, Berná,G, Rojas,A, Martín,F] Andalusian Center of Molecular Biology and Regenerative Medicine CABIMER, University Pablo Olavide-University of Seville-CSIC, Seville, Spain. [Álvarez-Amor,L, Hmadcha,A, Martín,F] Biomedical Research Network On Diabetes and Related Metabolic Diseases CIBERDEM, Instituto de Salud Carlos III, Madrid, Spain. [Gallego-Durán,R, Romero-Gómez,M] Hospital Universitario Virgen del Rocío de Sevilla, Instituto de Biomedicina de Sevilla, Universidad de Sevilla, Sevilla, Spain. [Gallego-Durán,R, Romero-Gómez,M] Biomedical Research Network On Hepatic and Digestive Diseases CIBEREHD, Instituto de Salud Carlos III, Madrid, Spain. [Robles-Frías,MJ] Compared Pathology Unit, Hospital Universitario Virgen del Rocío Intituto de Biomedicina de Sevilla Biobank Node, Andalucia Health Public System Biobank, Sevilla, Spain. [Kleemann,R] Department of Metabolic Health Research, Netherlands Organisation for Applied Scientifc Research-TNO, Leiden, The Netherlands. [Kleemann,R] Department of Vascular Surgery, Leiden University Medical Centre, Leiden, The Netherlands., and This work was supported by the grants AGL2014-54585-R, AGL-2017-86927-R, CP14/ 00105, PI18/01590 (Ministerio de Economía y Competitividad), PAI-BIO311 (Junta de Andalucía), CB07/08/0006 (Instituto de Salud Carlos III) and by the TNO research programs 'Body Brain Interactions ERP 020' and 'Functional Biomarkers PMC13'.
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0301 basic medicine ,Anatomy::Digestive System::Liver [Medical Subject Headings] ,Mice, Obese ,Diet, Mediterranean ,Mice ,chemistry.chemical_compound ,0302 clinical medicine ,Phenomena and Processes::Metabolic Phenomena::Metabolism::Lipid Metabolism [Medical Subject Headings] ,Non-alcoholic Fatty Liver Disease ,Extra virgin olive oil ,Organisms::Eukaryota::Animals [Medical Subject Headings] ,Glucose homeostasis ,Non-alcoholic steatohepatitis ,Mice, Knockout ,Multidisciplinary ,medicine.diagnostic_test ,Chemistry ,Analytical, Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Nutrition Therapy::Diet Therapy::Diet, Mediterranean [Medical Subject Headings] ,Fatty liver ,Lipoproteínas LDL ,food and beverages ,Type 2 diabetes ,Insulin sensitivity ,Aceite de oliva ,Liver ,Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Receptors, Cell Surface::Receptors, Lipoprotein::Receptors, LDL [Medical Subject Headings] ,Medicine ,Female ,030211 gastroenterology & hepatology ,lipids (amino acids, peptides, and proteins) ,medicine.symptom ,medicine.medical_specialty ,Science ,Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice::Mice, Mutant Strains::Mice, Obese [Medical Subject Headings] ,steatohepatitis ,Diet, High-Fat ,Resistencia a la insulina ,Article ,Transaminase ,LDL ,03 medical and health sciences ,Insulin resistance ,Phenomena and Processes::Physiological Phenomena::Nutritional Physiological Phenomena::Diet::Diet, High-Fat [Medical Subject Headings] ,Diseases::Nutritional and Metabolic Diseases::Metabolic Diseases::Glucose Metabolism Disorders::Hyperinsulinism::Insulin Resistance [Medical Subject Headings] ,Internal medicine ,medicine ,Animals ,Obesity ,Olive Oil ,Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice [Medical Subject Headings] ,Fatty acid metabolism ,Body Weight ,Diet, high-fat ,Hígado graso ,nutritional and metabolic diseases ,Peso corporal ,Body weight ,Lipid Metabolism ,medicine.disease ,Diseases::Nutritional and Metabolic Diseases::Nutrition Disorders::Overnutrition::Obesity [Medical Subject Headings] ,030104 developmental biology ,Endocrinology ,Dieta alta en grasa ,Receptors, LDL ,Check Tags::Female [Medical Subject Headings] ,Diseases::Pathological Conditions, Signs and Symptoms::Signs and Symptoms::Body Weight [Medical Subject Headings] ,Insulin Resistance ,Steatohepatitis ,Lipid profile ,Weight gain ,Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice::Mice, Mutant Strains::Mice, Knockout [Medical Subject Headings] - Abstract
Dietary fatty acids play a role in the pathogenesis of obesity-associated non-alcoholic fatty liver disease (NAFLD), which is associated with insulin resistance (IR). Fatty acid composition is critical for IR and subsequent NAFLD development. Extra-virgin olive oil (EVOO) is the main source of monounsaturated fatty acids (MUFA) in Mediterranean diets. This study examined whether EVOO-containing high fat diets may prevent diet-induced NAFLD using Ldlr−/−. Leiden mice. In female Ldlr−/−.Leiden mice, the effects of the following high fat diets (HFDs) were examined: a lard-based HFD (HFD-L); an EVOO-based HFD (HFD-EVOO); a phenolic compounds-rich EVOO HFD (HFD-OL). We studied changes in body weight (BW), lipid profile, transaminases, glucose homeostasis, liver pathology and transcriptome. Both EVOO diets reduced body weight (BW) and improved insulin sensitivity. The EVOOs did not improve transaminase values and increased LDL-cholesterol and liver collagen content. EVOOs and HFD-L groups had comparable liver steatosis. The profibrotic effects were substantiated by an up-regulation of gene transcripts related to glutathione metabolism, chemokine signaling and NF-kappa-B activation and down-regulation of genes relevant for fatty acid metabolism. Collectivelly, EVOO intake improved weight gain and insulin sensitivity but not liver inflammation and fibrosis, which was supported by changes in hepatic genes expression., This work was supported by the grants AGL2014-54585-R, AGL-2017-86927-R, CP14/ 00105, PI18/01590 (Ministerio de Economía y Competitividad), PAI-BIO311 (Junta de Andalucía), CB07/08/0006 (Instituto de Salud Carlos III) and by the TNO research programs “Body Brain Interactions ERP 020” and “Functional Biomarkers PMC13”.
- Published
- 2021