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In this study, real marine sediments polluted by petroleum compounds were treated by means of a bioslurry pilot scale reactor. The treatment performance was evaluated by measuring the removal of total petroleum hydrocarbon (TPH), coupled to further analyses required to understand the mechanisms involved in the biodegradation process. The maximum TPH-removal efficiency reached 86 % at the end of experiments. Moreover, high throughput 16S RNA gene sequencing was used to describe the microbiome composition in sediment prior to, and after, bioslurry treatment, in order to identify the taxa mostly entailed in the TPH removal process. The raw sediment was mostly colonized by members of Sulfurimonas genus; after bioslurry treatment, it was noticed a shift in the microbial community composition, with Proteobacteria phylum dominating the remediation environment (high increase in terms of growth for Hydrogenophaga and Sphingorhabdus genera) along with the Phaeodactylibacter genus (Bacteroidetes). Furthermore, the assessment of gaseous emissions from the system allowed to quantify the volatile hydrocarbon component and, consequently, to obtain a more accurate evaluation of TPH-removal pathway by the bioslurry system. Finally, phytotoxicity tests on sediment samples highlighted an increase of the treated sample quality status compared to the untreated one., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Contaminated marine sediments represent a critical threat towards human health and ecosystems, since they constitute a potential reservoir of toxic compounds release. In the present study, a bioslurry reactor was studied for the treatment of real marine sediments contaminated by petroleum hydrocarbons. The experimental campaign was divided in two periods: in the first period, microcosm trials were carried out to achieve useful indicators for biological hydrocarbon removal from sediments. The microcosm trials highlighted that the inoculum of halotolerant allochthonous bacteria provided the highest performance followed by autochthonous biomass. Based on the achieved results, in the second experimental period a bioslurry reactor was started up, based on a semisolid stirred tank reactor (STR) operated in batch mode. The process performances have been evaluated in terms of total petroleum hydrocarbon (TPH) removal, coupled with the characterization of microbial community through a Next Generation Sequencing (NGS) and phytotoxicity tests through the Germination Index (GI) with Lepidium Sativum seeds. The achieved results showed good hydrocarbons removal, equal to 40%, with a maximum removal rate of 220 mg TPH kg -1 d -1 , but highlighting that high contaminant concentrations might affect negatively the overall removal performance. In general, the observed results were encouraging towards the feasibility of biological treatment of marine sediments contaminated by hydrocarbons. The microbiological analysis allowed the identification of taxa most involved in the degradation of TPH, highlighting after the treatment a shift in the microbial community from that of the raw sediment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

In this study, a hydrocarbon-contaminated marine sediment was treated applying ex-situ thermal desorption (ESTD) at bench-scale. Temperatures up to 280 °C and heating times (t) in the 5-30 min range were investigated. Results revealed that temperatures in the range 200-280 °C led to Total Petrol Hydrocarbon (TPH)-removal efficiency (RE) from 75 to 85% (t = 10 min). The maximum RE of 89% was obtained at 200 °C for 30 min. However, a shorter remediation time of 5 min (or lower temperatures of 160 and 180 °C with longer times) is needed to reach the TPH standard limit. Data also demonstrated the selectivity of the treatment in TPH fraction removal. The modelling of the TPH removal kinetics and desorption isotherm jointly with activation energy calculation (>30 kJ mol -1 ) indicated that ESTD process is quite unfavorable for marine sediments. This is due to the fact that ESTD is regulated by chemisorption processes and occurred in two distinct TPH removal phases: evaporation and boiling vaporization. This depends on the strong affinity of the TPH with the fine sediment particles, as well as on the high initial water, salinity, organic matter and sulfides content. However, the comparison between alternative processes has shown that ESTD is the most feasible treatment process for TPH-contaminated marine sediment remediation. Obtained results also add relevant information that can be used as a basis for future scaling-up investigations of ESTD for hydrocarbon-contaminated marine sediments., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)