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1. Differential hRad17 expression by histologic subtype of ovarian cancer

Author

Young Jennifer L, Koon E Colin, Kwong Joseph, Welch William R, Muto Michael G, Berkowitz Ross S, and Mok Samuel C

Subjects
Gynecology and obstetrics, RG1-991
Abstract

Abstract Background In the search for unique ovarian cancer biomarkers, ovarian specific cDNA microarray analysis identified hRad17, a cell cycle checkpoint protein, as over-expressed in ovarian cancer. The aim of this study was to validate this expression. Methods Immunohistochemistry was performed on 72 serous, 19 endometrioid, 10 clear cell, and 6 mucinous ovarian cancers, 9 benign ovarian tumors, and 6 normal ovarian tissue sections using an anti-hRad17 antibody. Western blot analysis and quantitative PCR were performed using cell lysates and total RNA prepared from 17 ovarian cancer cell lines and 6 normal ovarian epithelial cell cultures (HOSE). Results Antibody staining confirmed upregulation of hRad17 in 49.5% of ovarian cancer cases. Immunohistochemistry demonstrated that only 42% of serous and 47% of endometrioid subtypes showed overexpression compared to 80% of clear cell and 100% of mucinous cancers. Western blot confirmed overexpression of hRad17 in cancer cell lines compared to HOSE. Quantitative PCR demonstrated an upregulation of hRad17 RNA by 1.5-7 fold. hRad17 RNA expression differed by subtype. Conclusions hRad17 is over-expressed in ovarian cancer. This over-expression varies by subtype suggesting a role in the pathogenesis of these types. Functional studies are needed to determine the potential role of this protein in ovarian cancer.

Published
2011
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2. Unique molecular characteristics of NAFLD-associated liver cancer accentuate β-catenin/TNFRSF19-mediated immune evasion

Author

Wong, Alissa Michelle, Ding, Xiaofan, Wong, Aikha Melissa, Xu, Mingjing, Zhang, Luyao, Leung, Howard Ho-Wai, Chan, Anthony Wing-Hung, Song, Qi Xiu, Kwong, Joseph, Chan, Loucia Kit-Ying, Man, Matthew, He, Mian, Chen, Jinna, Zhang, Zhe, You, Wenxing, Lau, Coleen, Yu, Allen, Wei, Yingying, Yuan, Yunfei, Lai, Paul Bo-San, Zhao, Jingmin, Man, Kwan, Yu, Jun, Kahn, Michael, and Wong, Nathalie

Published
2022
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19. Data from A Dynamic Inflammatory Cytokine Network in the Human Ovarian Cancer Microenvironment

Author

Kulbe, Hagen, primary, Chakravarty, Probir, primary, Leinster, D. Andrew, primary, Charles, Kellie A., primary, Kwong, Joseph, primary, Thompson, Richard G., primary, Coward, Jermaine I., primary, Schioppa, Tiziana, primary, Robinson, Stephen C., primary, Gallagher, William M., primary, Galletta, Laura, primary, Salako, Michael A., primary, Smyth, John F., primary, Hagemann, Thorsten, primary, Brennan, Donal J., primary, Bowtell, David D., primary, and Balkwill, Frances R., primary

Published
2023
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20. Supplementary Table 2 from A Dynamic Inflammatory Cytokine Network in the Human Ovarian Cancer Microenvironment

Author

Kulbe, Hagen, primary, Chakravarty, Probir, primary, Leinster, D. Andrew, primary, Charles, Kellie A., primary, Kwong, Joseph, primary, Thompson, Richard G., primary, Coward, Jermaine I., primary, Schioppa, Tiziana, primary, Robinson, Stephen C., primary, Gallagher, William M., primary, Galletta, Laura, primary, Salako, Michael A., primary, Smyth, John F., primary, Hagemann, Thorsten, primary, Brennan, Donal J., primary, Bowtell, David D., primary, and Balkwill, Frances R., primary

Published
2023
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21. Supplementary Figure Legends 1-4 from A Dynamic Inflammatory Cytokine Network in the Human Ovarian Cancer Microenvironment

Author

Kulbe, Hagen, primary, Chakravarty, Probir, primary, Leinster, D. Andrew, primary, Charles, Kellie A., primary, Kwong, Joseph, primary, Thompson, Richard G., primary, Coward, Jermaine I., primary, Schioppa, Tiziana, primary, Robinson, Stephen C., primary, Gallagher, William M., primary, Galletta, Laura, primary, Salako, Michael A., primary, Smyth, John F., primary, Hagemann, Thorsten, primary, Brennan, Donal J., primary, Bowtell, David D., primary, and Balkwill, Frances R., primary

Published
2023
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22. Supplementary Figure 3 from A Dynamic Inflammatory Cytokine Network in the Human Ovarian Cancer Microenvironment

Author

Kulbe, Hagen, primary, Chakravarty, Probir, primary, Leinster, D. Andrew, primary, Charles, Kellie A., primary, Kwong, Joseph, primary, Thompson, Richard G., primary, Coward, Jermaine I., primary, Schioppa, Tiziana, primary, Robinson, Stephen C., primary, Gallagher, William M., primary, Galletta, Laura, primary, Salako, Michael A., primary, Smyth, John F., primary, Hagemann, Thorsten, primary, Brennan, Donal J., primary, Bowtell, David D., primary, and Balkwill, Frances R., primary

Published
2023
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23. Supplementary Table 1 from A Dynamic Inflammatory Cytokine Network in the Human Ovarian Cancer Microenvironment

Author

Kulbe, Hagen, primary, Chakravarty, Probir, primary, Leinster, D. Andrew, primary, Charles, Kellie A., primary, Kwong, Joseph, primary, Thompson, Richard G., primary, Coward, Jermaine I., primary, Schioppa, Tiziana, primary, Robinson, Stephen C., primary, Gallagher, William M., primary, Galletta, Laura, primary, Salako, Michael A., primary, Smyth, John F., primary, Hagemann, Thorsten, primary, Brennan, Donal J., primary, Bowtell, David D., primary, and Balkwill, Frances R., primary

Published
2023
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24. Supplementary Figure 4 from A Dynamic Inflammatory Cytokine Network in the Human Ovarian Cancer Microenvironment

Author

Kulbe, Hagen, primary, Chakravarty, Probir, primary, Leinster, D. Andrew, primary, Charles, Kellie A., primary, Kwong, Joseph, primary, Thompson, Richard G., primary, Coward, Jermaine I., primary, Schioppa, Tiziana, primary, Robinson, Stephen C., primary, Gallagher, William M., primary, Galletta, Laura, primary, Salako, Michael A., primary, Smyth, John F., primary, Hagemann, Thorsten, primary, Brennan, Donal J., primary, Bowtell, David D., primary, and Balkwill, Frances R., primary

Published
2023
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25. Supplementary Figure 2 from A Dynamic Inflammatory Cytokine Network in the Human Ovarian Cancer Microenvironment

Author

Kulbe, Hagen, primary, Chakravarty, Probir, primary, Leinster, D. Andrew, primary, Charles, Kellie A., primary, Kwong, Joseph, primary, Thompson, Richard G., primary, Coward, Jermaine I., primary, Schioppa, Tiziana, primary, Robinson, Stephen C., primary, Gallagher, William M., primary, Galletta, Laura, primary, Salako, Michael A., primary, Smyth, John F., primary, Hagemann, Thorsten, primary, Brennan, Donal J., primary, Bowtell, David D., primary, and Balkwill, Frances R., primary

Published
2023
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26. Supplementary Figure 1 from A Dynamic Inflammatory Cytokine Network in the Human Ovarian Cancer Microenvironment

Author

Kulbe, Hagen, primary, Chakravarty, Probir, primary, Leinster, D. Andrew, primary, Charles, Kellie A., primary, Kwong, Joseph, primary, Thompson, Richard G., primary, Coward, Jermaine I., primary, Schioppa, Tiziana, primary, Robinson, Stephen C., primary, Gallagher, William M., primary, Galletta, Laura, primary, Salako, Michael A., primary, Smyth, John F., primary, Hagemann, Thorsten, primary, Brennan, Donal J., primary, Bowtell, David D., primary, and Balkwill, Frances R., primary

Published
2023
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28. Genomic aberrations in cervical adenocarcinomas in Hong Kong Chinese women

Author

Chung, Tony K.H., Van Hummelen, Paul, Chan, Paul K.S., Cheung, Tak Hong, Yim, So Fan, Yu, Mei Y., Ducar, Matthew D., Thorner, Aaron R., MacConaill, Laura E., Doran, Graeme, Pedamallu, Chandra Sekhar, Ojesina, Akinyemi I., Wong, Raymond R.Y., Wang, Vivian W., Freeman, Samuel S., Lau, Tat San, Kwong, Joseph, Chan, Loucia K.Y., Fromer, Menachem, May, Taymaa, Worley, Michael J., Jr., Esselen, Katharine M., Elias, Kevin M., Lawrence, Michael, Getz, Gad, Smith, David I., Crum, Christopher P., Meyerson, Matthew, Berkowitz, Ross S., and Wong, Yick Fu

Published
2015
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32. Long-Term Survival and Clinicopathological Implications of DNA Mismatch Repair Status in Endometrioid Endometrial Cancers in Hong Kong Chinese Women

Author

Lee, Jacqueline Ho Sze, primary, Li, Joshua Jing Xi, additional, Chow, Chit, additional, Chan, Ronald Cheong Kin, additional, Kwan, Johnny Sheung Him, additional, Lau, Tat San, additional, To, Ka Fai, additional, Yim, So Fan, additional, Yeung, Suet Ying, additional, and Kwong, Joseph, additional

Published
2021
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35. Claudin-4 Overexpression in Epithelial Ovarian Cancer Is Associated with Hypomethylation and Is a Potential Target for Modulation of Tight Junction Barrier Function Using a C-Terminal Fragment of Clostridium perfringens Enterotoxin

Author

Litkouhi, Babak, Kwong, Joseph, Lot, Chun-Min, Smedley, James G., III, McClane, Bruce A., Aponte, Margarita, Gao, Zhijian, Sarno, Jennifer L., Hinners, Jennifer, Welch, William R., Berkowitz, Ross S., Mok, Samuel C., and Garner, Elizabeth I.O.

Published
2007
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36. Short-Form Thymic Stromal Lymphopoietin (sfTSLP) Is the Predominant Isoform Expressed by Gynaecologic Cancers and Promotes Tumour Growth

Author

Chan, Loucia Kit Ying, primary, Lau, Tat San, additional, Chung, Kit Ying, additional, Tam, Chit, additional, Cheung, Tak Hong, additional, Yim, So Fan, additional, Lee, Jacqueline Ho Sze, additional, Leung, Ricky Wai Tak, additional, Qin, Jing, additional, Or, Yvonne Yan Yan, additional, Lo, Kwok Wai, additional, and Kwong, Joseph, additional

Published
2021
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40. Abstract 3911: High-throughput screening of patient-derived HCC organoids for drug discovery

Author

Wong, Alissa Michelle Go, primary, Cheung, Winnie, additional, Wong, Maggie, additional, Wong, Aikha, additional, Lam, Helena, additional, Zhang, Zhe, additional, Man, Matthew, additional, Chen, Hui, additional, Close, David, additional, Johnston, Paul, additional, Chan, Anthony, additional, Lai, Paul, additional, Kwong, Joseph, additional, and Wong, Nathalie, additional

Published
2020
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43. Additional file 2 of Therapeutic potential of a novel prodrug of green tea extract in induction of apoptosis via ERK/JNK and Akt signaling pathway in human endometrial cancer

Author

Man, Gene Chi Wai, Jianzhang Wang, Song, Yi, Wong, Jack Ho, Zhao, Yu, Lau, Tat San, Leung, Kam Tong, Chan, Tak Hang, Huating Wang, Kwong, Joseph, Tzi Bun Ng, and Wang, Chi Chiu

Abstract

Additional file 2 Supplementary Figure 2. ProEGCG suppresses colony formation of endometrial cancer cells in 3D organoid co-culture. Endometrial cancer cells (AN3CA: 10,000 cells. RL95–2: 20,000 cells) were seeded onto matrigel. Cells were treated with either EGCG or ProEGCG (A) from day 2 to day 6, or (B) from day 7 to day 11. Photographs of spheroid sizes were taken under the microscope (100X), and the area of spheroids was measured. Data are presented as mean ± S.E.M. of three independent experiments. Significant differences from the control are indicated by * (P

Published
2020
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44. Additional file 3 of Therapeutic potential of a novel prodrug of green tea extract in induction of apoptosis via ERK/JNK and Akt signaling pathway in human endometrial cancer

Author

Man, Gene Chi Wai, Jianzhang Wang, Song, Yi, Wong, Jack Ho, Zhao, Yu, Lau, Tat San, Leung, Kam Tong, Chan, Tak Hang, Huating Wang, Kwong, Joseph, Tzi Bun Ng, and Wang, Chi Chiu

Subjects
sense organs
Abstract

Additional file 3 Supplementary Figure 3. Immunofluorescence staining for detection of apoptosis in human endometrial carcinoma cell lines. (A) RL95–2 and AN3CA cells were treated with increasing doses of EGCG (20, 40, and 60 μM), or (B) ProEGCG (20, 40, and 60 μM) for 72 h with daily medium change and supplement. Localization and expression of Annexin-V (green) and propidium iodide (red) were analyzed by immunofluorescent staining. Nuclei were counterstained using DAPI (blue).

Published
2020
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45. Additional file 5 of Therapeutic potential of a novel prodrug of green tea extract in induction of apoptosis via ERK/JNK and Akt signaling pathway in human endometrial cancer

Author

Man, Gene Chi Wai, Jianzhang Wang, Song, Yi, Wong, Jack Ho, Zhao, Yu, Lau, Tat San, Leung, Kam Tong, Chan, Tak Hang, Huating Wang, Kwong, Joseph, Tzi Bun Ng, and Wang, Chi Chiu

Abstract

Additional file 5 Supplementary Figure 5. Full Western Blot Images from Figs. 3 and 4. Western blot analysis of the relative protein levels of Rl95–2 and AN3 CA cells. Quantitative analysis of the relative protein levels of Rl95–2 and AN3 CA cells was carried out in triplicate and repeated in 3 independent experiments. Rl95–2 and AN3 CA cells were probed with cleaved PARP, cleaved Caspase, GAPDH antibodies. Molecular weight (kDa) markers (MW) are shown: cleaved PARP is 116 kDa, cleaved Caspase-3 is 17/19 kDa, p-Akt is 60 kDa, Akt is 60 kDa, p-JNK is 54/46 kDa, JNK is 54/46 kDa, p-ERK is 44/42 kDa, ERK is 44/42 kDa, p-p38 is 43 kDa, p38 is 43 kDa, and GAPDH is 37 kDa.

Published
2020
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46. Additional file 4 of Therapeutic potential of a novel prodrug of green tea extract in induction of apoptosis via ERK/JNK and Akt signaling pathway in human endometrial cancer

Author

Man, Gene Chi Wai, Jianzhang Wang, Song, Yi, Wong, Jack Ho, Zhao, Yu, Lau, Tat San, Leung, Kam Tong, Chan, Tak Hang, Huating Wang, Kwong, Joseph, Tzi Bun Ng, and Wang, Chi Chiu

Subjects
animal structures, embryonic structures
Abstract

Additional file 4 Supplementary Figure 4. Positive Control of TUNEL staining. Mouse embryo E10 brain tissue sample was collected and incubated with a brief treatment of DNAse 1 after permeabilization. This was then proceeded with the TUNEL assay accordingly. Magnification 200x.

Published
2020
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47. Additional file 1 of Therapeutic potential of a novel prodrug of green tea extract in induction of apoptosis via ERK/JNK and Akt signaling pathway in human endometrial cancer

Author

Man, Gene Chi Wai, Jianzhang Wang, Song, Yi, Wong, Jack Ho, Zhao, Yu, Lau, Tat San, Leung, Kam Tong, Chan, Tak Hang, Huating Wang, Kwong, Joseph, Tzi Bun Ng, and Wang, Chi Chiu

Abstract

Additional file 1 Supplementary Figure 1. Effects of ProEGCG and EGCG on viability of normal human endometrial epithelial cells (HEECs). (A) HEECs were treated with increasing doses of EGCG (20, 40, and 60 μM) and ProEGCG (20, 40, and 60 μM) for 24, 48 and 72 h. Cell viability was assessed by MTT assay. The percentage of viable cells was calculated as the ratio of treated cells to the control cells. Data are presented as mean ± S.E.M. of three independent experiments. Significant differences from the control are indicated by * (P

Published
2020
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50. MDSCs drive the process of endometriosis by enhancing angiogenesis and provide a new potential approach for treatment

Author

Zhang, Tao, Zhou, Juhua, Man, Gene Chi Wai, Leung, Kam Tong, Liang, Bo, Xiao, Bo, Ma, Xinting, Huang, Shaoyan, Huang, Huaxiang, Hegde, Venkatesh L., Zhong, Yin, Li, Yanmin, Kong, Grace Wing Shan, Yiu, Alice KaWah, Kwong, Joseph, Ng, Pak Cheung, Nagarkatti, Prakash S., Nagarkatti, Mitzi, and Wang, Chi Chiu

Subjects
Mice, Knockout, Arginase, Myeloid-Derived Suppressor Cells, Endometriosis, Article, Receptors, Interleukin-8B, Mice, Inbred C57BL, Disease Models, Animal, Endometrium, Mice, Animals, Cytokines, Humans, Angiogenesis Inducing Agents, Female, Chemokines, Reactive Oxygen Species, Cells, Cultured, Granulocytes
Abstract

Endometriosis affects women of reproductive age via unclear immunological mechanism(s). Myeloid-derived suppressor cells (MDSCs) are a heterogeneous group of myeloid cells with potent immunosuppressive and angiogenic properties. Here, we found MDSCs significantly increased in the peripheral blood of patients with endometriosis and in the peritoneal cavity of a mouse model of surgically induced endometriosis. Majority of MDSCs were granulocytic, produced ROS, and arginase, and suppressed T-cell proliferation. Depletion of MDSCs by antiGr-1 antibody dramatically suppressed development of endometrial lesions in mice. The chemokines CXCL1, 2, and 5 were expressed at sites of lesion while MDSCs expressed CXCR-2. These CXC-chemokines promoted MDSC migration toward endometriotic implants both in vitro and in vivo. Also, CXCR2-deficient mice show significantly decreased MDSC induction, endometrial lesions, and angiogenesis. Importantly, adoptive transfer of MDSCs into CXCR2-KO mice restored endometriotic growth and angiogenesis. Together, this study demonstrates that MDSCs play a role in the pathogenesis of endometriosis and identifies a novel CXC-chemokine and receptor for the recruitment of MDSCs, thereby providing a potential target for endometriosis treatment.

Published
2018

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