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36 results on '"Kwok, Hin-Fai"'

8. Pro-angiogenic effects of Carthami Flos whole extract in human microvascular endothelial cells in vitro and in zebrafish in vivo

Author

Zhou, Xuelin, Siu, Wing-Sum, Fung, Chak-Hei, Cheng, Ling, Wong, Chun-Wai, Zhang, Cheng, Liu, Cheuk-Lun, Kwok, Hin-Fai, Lau, Ching-Po, Wat, Elaine, Lau, Clara Bik-San, Leung, Ping-Chung, Ko, Chun-Hay, and Hung, Leung-Kim

Subjects
Materia medica, Vegetable -- Health aspects, Neovascularization -- Research, Pharmacology, Experimental, Medicine, Botanic -- Research, Zebra fish -- Physiological aspects, Medicine, Herbal -- Research, Plant extracts -- Health aspects, Biological sciences, Health, Science and technology
Abstract

ABSTRACT Aim: Carthami Flos (CF) is a Chinese herb traditionally used fo r cardiovascular disease and bone injury in China with pharmacological effects on improving blood circulation. The aim of [...]

Published
2014
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15. Multiple modulatory activities of Andrographis paniculata on immune responses and xenograft growth in esophageal cancer preclinical models

Author

Yue, Grace Gar-Lee, primary, Li, Lin, additional, Lee, Julia Kin-Ming, additional, Kwok, Hin-Fai, additional, Wong, Eric Chun-Wai, additional, Li, Mingyue, additional, Fung, Kwok-Pui, additional, Yu, Jun, additional, Chan, Anthony Wing-Hung, additional, Chiu, Philip Wai-Yan, additional, and Lau, Clara Bik-San, additional

Published
2019
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18. Whole extracts of Radix Achyranthis Bidentatae and Radix Cyathulae promote angiogenesis in human umbilical vein endothelial cells in vitro and in zebrafish in vivo

Author

Zhou, Xuelin, primary, Siu, Wing-Sum, additional, Zhang, Cheng, additional, Liu, Cheuk-Lun, additional, Cheng, Ling, additional, Kwok, Hin-Fai, additional, Fung, Chak-Hei, additional, Tam, Jacqueline Chor-Wing, additional, Lau, Ching-Po, additional, Lau, Clara Bik-San, additional, Leung, Ping-Chung, additional, Hung, Leung-Kim, additional, and Ko, Chun-Hay, additional

Published
2017
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20. Combined therapy using bevacizumab and turmeric ethanolic extract (with absorbable curcumin) exhibited beneficial efficacy in colon cancer mice

Author

Yue, Grace Gar-Lee, primary, Kwok, Hin-Fai, additional, Lee, Julia Kin-Ming, additional, Jiang, Lei, additional, Wong, Eric Chun-Wai, additional, Gao, Si, additional, Wong, Hing-Lok, additional, Li, Lin, additional, Chan, Kar-Man, additional, Leung, Ping-Chung, additional, Fung, Kwok-Pui, additional, Zuo, Zhong, additional, and Lau, Clara Bik-San, additional

Published
2016
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22. Novel PI3K/AKT targeting anti-angiogenic activities of 4-vinylphenol, a new therapeutic potential of a well-known styrene metabolite

Author

Yue, Grace Gar-Lee, primary, Lee, Julia Kin-Ming, additional, Kwok, Hin-Fai, additional, Cheng, Ling, additional, Wong, Eric Chun-Wai, additional, Jiang, Lei, additional, Yu, Hua, additional, Leung, Hoi-Wing, additional, Wong, Yuk-Lau, additional, Leung, Ping-Chung, additional, Fung, Kwok-Pui, additional, and Lau, Clara Bik-San, additional

Published
2015
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23. Enumeration and functional investigation of endothelial progenitor cells in neovascularization of diabetic foot ulcer rats with a Chinese 2-herb formula

Author

Tam, Jacqueline Chor Wing, primary, Ko, Chun Hay, additional, Lau, Kit Man, additional, To, Ming Ho, additional, Kwok, Hin Fai, additional, Siu, Wing Sum, additional, Lau, Ching Po, additional, Chan, Wai Yee, additional, Leung, Ping Chung, additional, Fung, Kwok Pui, additional, and Lau, Clara Bik San, additional

Published
2014
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28. Synergistic interaction between Astragali Radix and Rehmanniae Radix in a Chinese herbal formula to promote diabetic wound healing

Author

Lau, Kit-Man, primary, Lai, Kwok-Kin, additional, Liu, Cheuk-Lun, additional, Tam, Jacqueline Chor-Wing, additional, To, Ming-Ho, additional, Kwok, Hin-Fai, additional, Lau, Ching-Po, additional, Ko, Chun-Hay, additional, Leung, Ping-Chung, additional, Fung, Kwok-Pui, additional, Poon, Simon Kar-Sing, additional, and Lau, Clara Bik-San, additional

Published
2012
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30. Cloning and characterization of gonadotropin receptors in the zebrafish, danio rerio.

Author

Kwok, Hin-Fai., Chinese University of Hong Kong Graduate School. Division of Molecular Biotechnology., Kwok, Hin-Fai., and Chinese University of Hong Kong Graduate School. Division of Molecular Biotechnology.

Abstract

Kwok Hin-Fai., Thesis (M.Phil.)--Chinese University of Hong Kong, 2004., Includes bibliographical references (leaves 84-100)., s in English and Chinese., (in English) --- p.i, (in Chinese) --- p.iv, Acknowledgement --- p.vi, Table of contents --- p.vii, List of figures --- p.xi, List of tables --- p.xiv, Symbols and abbreviations --- p.xv, List of fish names mentioned in the thesis --- p.xviii, Chapter Chapter 1 --- General Introduction, Chapter 1.1 --- Gonadotropins, Chapter 1.1.1 --- Structure --- p.1, Chapter 1.1.2 --- Function --- p.4, Chapter 1.2 --- Gonadotropin receptor, Chapter 1.2.1 --- Structure --- p.5, Chapter 1.2.2 --- Expression --- p.7, Chapter 1.2.3 --- Signaling, Chapter 1.2.3.1 --- cAMP-mediated pathway --- p.7, Chapter 1.2.3.2 --- Phospholipase C-mediated pathway --- p.9, Chapter 1.2.4 --- Regulation of expression --- p.12, Chapter 1.2.5 --- Desensitization of receptors, Chapter 1.2.5.1 --- Uncoupling --- p.13, Chapter 1.2.5.2 --- Internalization --- p.13, Chapter 1.3 --- Structure of ovarian follicles --- p.14, Chapter 1.4 --- The project objectives and long-term significance --- p.16, Chapter Chapter 2 --- Cloning and Characterization of Zebrafish Follicle-stimulating Hormone (FSH) and Luteinizing Hormone (LH) Receptors ´ؤ Evidence for Distinct Functions of FSH and LH in Follicle Development, Chapter 2.1 --- Introduction --- p.19, Chapter 2.2 --- Materials and Methods, Chapter 2.2.1 --- Animals and chemicals --- p.22, Chapter 2.2.2 --- Isolation of total RNA --- p.22, Chapter 2.2.3 --- Cloning of zebrafish FSHR (zfFSHR) and LHR (zfLHR) cDNA fragments from the zebrafish ovary --- p.23, Chapter 2.2.4 --- Rapid amplification of 5´ةcDNA ends (5'-RACE) and full-length cDNA --- p.24, Chapter 2.2.5 --- Isolation of ovarian follicles --- p.25, Chapter 2.2.6 --- Sampling of the ovaries from sexually immature zebrafish --- p.25, Chapter 2.2.7 --- Reverse transcription-polymerase chain reaction (RT-PCR) --- p.25, Chapter 2.2.8 --- Construction of expression plasmids --- p.26, Chapter 2.2.9 --- Transient transfection and reporter gene assay --- p.27, Chapter 2.2.10 --- Establishment and characterization of stable zfFSHR or zfLHR-expressing cell lines --- p.28, Chapter 2.3 --- Results, Chapter 2.3.1 --- Cloning of FSHR and LHR cDNA from the zebrafish ovary --- p.29, Chapter 2.3.2 --- Functional characterization of zfFSHR and zfLHR --- p.30, Chapter 2.3.3 --- Expression of zfFSHR and zfLHR during sexual maturation --- p.31, Chapter 2.3.4 --- Stage-dependent expression of zfFSHR and zfLHR in the ovarian follicles --- p.32, Chapter 2.4 --- Discussion --- p.33, Chapter Chapter 3 --- Down-regulation of FSHR and LHR Expression in the Zebrafish Follicle Ceils by Gonadotropin (hCG) and Its Sigaling Mechanism, Chapter 3.1 --- Introduction --- p.51, Chapter 3.2 --- Materials and Methods, Chapter 3.2.1 --- Animals --- p.54, Chapter 3.2.2 --- Chemicals and hormones --- p.54, Chapter 3.2.3 --- Primary follicle cell culture --- p.55, Chapter 3.2.4 --- Total RNA isolation --- p.55, Chapter 3.2.5 --- "Validation of semi-quantitative RT-PCR assays for FSHR, LHR and GAPDH" --- p.56, Chapter 3.2.6 --- Data analysis --- p.57, Chapter 3.3 --- Results, Chapter 3.3.1 --- Validation of semi-quantitative RT-PCR assays --- p.57, Chapter 3.3.2 --- Gonadotropin regulation of FSHR and LHR expression in cultured zebrafish ovarian follicle cells --- p.58, Chapter 3.3.3 --- Effect of db-cAMP and forskolin on FSHR and LHR expression --- p.59, Chapter 3.3.4 --- Effects of H89 on hCG-induced suppression of FSHR and LHR expression --- p.60, Chapter 3.4 --- Discussion --- p.60, Chapter Chapter 4 --- General Discussion --- p.75, Chapter 4.1 --- Cloning of zebrafish FSHR and LHR cDNAs and demonstration of receptor specificity --- p.77, Chapter 4.2 --- Evidence for the differential expression of FSHR and LHR in the zebrafish ovarian and follicle development --- p.78, Chapter 4.3 --- Down-regulation of FSHR and LHR expression in the zebrafish follicle cells by gonadotropin (hCG) --- p.79, Chapter 4.4 --- Future research direction --- p.80, References --- p.84, http://library.cuhk.edu.hk/record=b5896371, Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

Published
2004

32. Bioassay-guided isolation of norviburtinal from the root of Rehmannia glutinosa, exhibited angiogenesis effect in zebrafish embryo model

Author

Liu, Cheuk-Lun, primary, Cheng, Ling, additional, Kwok, Hin-Fai, additional, Ko, Chun-Hay, additional, Lau, Tai-Wai, additional, Koon, Chi-Man, additional, Zhao, Ming, additional, Lau, Ching-Po, additional, Lau, Kit-Man, additional, Wong, Chun-Wai, additional, Leung, Ping-Chung, additional, Fung, Kwok-Pui, additional, and Lau, Clara Bik-San, additional

Published
2011
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35. Induction of Angiogenesis in Zebrafish Embryos and Proliferation of Endothelial Cells by an Active Fraction Isolated from the Root of Astragalus membranaceususing Bioassay-guided Fractionation

Author

Lai, Patrick Kwok-Kin, Chan, Judy Yuet-Wa, Kwok, Hin-Fai, Cheng, Ling, Yu, Hua, Lau, Ching-Po, Leung, Ping-Chung, Fung, Kwok-Pui, and Lau, Clara Bik-San

Abstract

The objective of the study was to identify the active fraction(s) from AR aqueous extract responsible for promoting angiogenesis using bioassay-guided fractionation. The angiogenic activity was screened by monitoring the increase of sprout number in sub-intestinal vessel (SIV) of the transgenic zebrafish embryos after they were treated with 0.06-0.25mg/ml of AR aqueous extract or its fraction(s) for 96h. Furthermore, the angiogenic effect was evaluated in treated zebrafish embryos by measuring the gene expression of angiogenic markers (VEGFA, KDR, and Flt-1) using real-time polymerase chain reaction (RT-PCR), and in human microvascular endothelial cell (HMEC-1) by measuring cell proliferation using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, 3H-thymidine uptake assay, and cell cycle analysis. A major active fraction (P1-1-1), which was identified as glycoproteins, was found to significantly stimulate sprout formation (2.03±0.27) at 0.125mg/ml (P<0.001) and up-regulate the gene expression of VEGFA, KDR, and Flt-1 by 2.6-fold to 8.2-fold. Additionally, 0.031-0.125mg/ml of P1-1-1 was demonstrated to significantly stimulate cell proliferation by increasing cell viability (from 180% to 205%), 3H-thymidine incorporation (from 126% to 133%) during DNA synthesis, and the shift of cell population to S phase of cell cycle. A major AR active fraction consisting of glycoproteins was identified, and shown to promote angiogenesis in zebrafish embryos and proliferation of endothelial cells in vitro.

Published
2014
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36. Method establishment for upgrading chemical markers in pharmacopoeia to bioactive markers for biological standardization of traditional Chinese medicine.

Author

Lau CB, Yue GG, Lau KM, Chan YY, Shaw PC, Kwok HF, and Wong LS

Abstract

Quality surveillance on authentication, safety and efficacy of proprietary Chinese medicines (pCm) are certainly the top priorities for the industries. Nowadays, the quality control system adopted is mainly chemical marker-oriented, concerning basically the correct use of raw material and safety issues, while the biological activities of the chemical marker(s) are seldom considered. Hence, there is an undefined relationship between the amount of chemical markers and the claimed pharmacological activities. In view of the need in identifying appropriate markers for biological standardization of pCm products, the present study aimed to establish a systematic methodology for verifying whether the chemical marker of a traditional Chinese medicine (TCM) listed in Chinese Pharmacopoeia could be upgraded to a bioactive marker with certain efficacy in treating a particular disease. Our proposed methodology included a series of work on extraction, quantification, literature search and in vivo pharmacological experiments, in which the water extractability, biological effects at theoretical dose and oral bioavailability of the candidate chemical markers were all taken into consideration. The feasibility and implication of this bioactive markers verification methodology were further elaborated. Our findings will serve as the foundation for further research and development of biological standardization of TCM.

Published
2018
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