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155 results on '"Kwak, Seon-Yeong"'

4. A Nanobionic Light-Emitting Plant

Author

Kwak, Seon-Yeong, Giraldo, Juan Pablo, Wong, Min Hao, Koman, Volodymyr B, Lew, Tedrick Thomas Salim, Ell, Jon, Weidman, Mark C, Sinclair, Rosalie M, Landry, Markita P, Tisdale, William A, and Strano, Michael S

Subjects
Nanotechnology, Bioengineering, Affordable and Clean Energy, Brassicaceae, Cadmium Compounds, Chitosan, Coenzyme A, Firefly Luciferin, Light, Luciferases, Luminescence, Luminescent Agents, Nanoparticles, Nasturtium, Plant Leaves, Polylactic Acid-Polyglycolic Acid Copolymer, Radiation, Selenium Compounds, Spinacia oleracea, Plant nanobionics, nanoparticles, pressurized bath infusion of nanoparticles, light-emitting plant, chemiluminescence, Nanoscience & Nanotechnology
Abstract

The engineering of living plants for visible light emission and sustainable illumination is compelling because plants possess independent energy generation and storage mechanisms and autonomous self-repair. Herein, we demonstrate a plant nanobionic approach that enables exceptional luminosity and lifetime utilizing four chemically interacting nanoparticles, including firefly luciferase conjugated silica (SNP-Luc), d-luciferin releasing poly(lactic-co-glycolic acid) (PLGA-LH2), coenzyme A functionalized chitosan (CS-CoA) and semiconductor nanocrystal phosphors for longer wavelength modulation. An in vitro kinetic model incorporating the release rates of the nanoparticles is developed to maximize the chemiluminescent lifetimes to exceed 21.5 h. In watercress (Nasturtium officinale) and other species, the nanoparticles circumvent limitations such as luciferin toxicity above 400 μM and colocalization of enzymatic reactions near high adenosine triphosphate (ATP) production. Pressurized bath infusion of nanoparticles (PBIN) is introduced to deliver a mixture of nanoparticles to the entire living plant, well described using a nanofluidic mathematical model. We rationally design nanoparticle size and charge to control localization within distinct tissues compartments with 10 nm nanoparticles localizing within the leaf mesophyll and stomata guard cells, and those larger than 100 nm segregated in the leaf mesophyll. The results are mature watercress plants that emit greater than 1.44 × 1012 photons/sec or 50% of 1 μW commercial luminescent diodes and modulate "off" and "on" states by chemical addition of dehydroluciferin and coenzyme A, respectively. We show that CdSe nanocrystals can shift the chemiluminescent emission to 760 nm enabling near-infrared (nIR) signaling. These results advance the viability of nanobionic plants as self-powered photonics, direct and indirect light sources.

Published
2017

5. Lipid Exchange Envelope Penetration (LEEP) of Nanoparticles for Plant Engineering: A Universal Localization Mechanism

Author

Wong, Min Hao, Misra, Rahul P, Giraldo, Juan P, Kwak, Seon-Yeong, Son, Youngwoo, Landry, Markita P, Swan, James W, Blankschtein, Daniel, and Strano, Michael S

Subjects
Biological Sciences, Biomedical and Clinical Sciences, Industrial Biotechnology, Engineering, Medical Biotechnology, Nanotechnology, Bioengineering, Charge-mediated, nanoparticles, single-particle tracking, single-walled carbon nanotubes, chloroplast, LEEP, Nanoscience & Nanotechnology
Abstract

Nanoparticles offer clear advantages for both passive and active penetration into biologically important membranes. However, the uptake and localization mechanism of nanoparticles within living plants, plant cells, and organelles has yet to be elucidated.1 Here, we examine the subcellular uptake and kinetic trapping of a wide range of nanoparticles for the first time, using the plant chloroplast as a model system, but validated in vivo in living plants. Confocal visible and near-infrared fluorescent microscopy and single particle tracking of gold-cysteine-AF405 (GNP-Cys-AF405), streptavidin-quantum dot (SA-QD), dextran and poly(acrylic acid) nanoceria, and various polymer-wrapped single-walled carbon nanotubes (SWCNTs), including lipid-PEG-SWCNT, chitosan-SWCNT and 30-base (dAdT) sequence of ssDNA (AT)15 wrapped SWCNTs (hereafter referred to as ss(AT)15-SWCNT), are used to demonstrate that particle size and the magnitude, but not the sign, of the zeta potential are key in determining whether a particle is spontaneously and kinetically trapped within the organelle, despite the negative zeta potential of the envelope. We develop a mathematical model of this lipid exchange envelope and penetration (LEEP) mechanism, which agrees well with observations of this size and zeta potential dependence. The theory predicts a critical particle size below which the mechanism fails at all zeta potentials, explaining why nanoparticles are critical for this process. LEEP constitutes a powerful particulate transport and localization mechanism for nanoparticles within the plant system.

Published
2016

7. A Ratiometric Sensor Using Single Chirality Near‐Infrared Fluorescent Carbon Nanotubes: Application to In Vivo Monitoring

Author

Giraldo, Juan P, Landry, Markita P, Kwak, Seon-Yeong, Jain, Rishabh M, Wong, Min Hao, Iverson, Nicole M, Ben-Naim, Micha, and Strano, Michael S

Subjects
Analytical Chemistry, Chemical Sciences, Engineering, Information and Computing Sciences, Data Management and Data Science, Arabidopsis, Biosensing Techniques, Fluorescence, Free Radicals, Hydrogen Peroxide, Infrared Rays, Nanotubes, Carbon, Nitric Oxide, Plant Leaves, Sonication, Suspensions, Time Factors, free radicals, optical nanosensors, reactive oxygen species, sensors, plant nanobionics, Nanoscience & Nanotechnology
Abstract

Advances in the separation and functionalization of single walled carbon nanotubes (SWCNT) by their electronic type have enabled the development of ratiometric fluorescent SWCNT sensors for the first time. Herein, single chirality SWCNT are independently functionalized to recognize either nitric oxide (NO), hydrogen peroxide (H(2)O(2)), or no analyte (remaining invariant) to create optical sensor responses from the ratio of distinct emission peaks. This ratiometric approach provides a measure of analyte concentration, invariant to the absolute intensity emitted from the sensors and hence, more stable to external noise and detection geometry. Two distinct ratiometric sensors are demonstrated: one version for H(2)O(2), the other for NO, each using 7,6 emission, and each containing an invariant 6,5 emission wavelength. To functionalize these sensors from SWCNT isolated from the gel separation technique, a method for rapid and efficient coating exchange of single chirality sodium dodecyl sulfate-SWCNT is introduced. As a proof of concept, spatial and temporal patterns of the ratio sensor response to H(2)O(2) and, separately, NO, are monitored in leaves of living plants in real time. This ratiometric optical sensing platform can enable the detection of trace analytes in complex environments such as strongly scattering media and biological tissues.

Published
2015

15. Polymethacrylamide and Carbon Composites that Grow, Strengthen, and Self‐Repair using Ambient Carbon Dioxide Fixation

Author

Kwak, Seon‐Yeong, Giraldo, Juan Pablo, Lew, Tedrick Thomas Salim, Wong, Min Hao, Liu, Pingwei, Yang, Yun Jung, Koman, Volodymyr B., McGee, Melissa K., Olsen, Bradley D., Strano, Michael S., Kwak, Seon‐Yeong, Giraldo, Juan Pablo, Lew, Tedrick Thomas Salim, Wong, Min Hao, Liu, Pingwei, Yang, Yun Jung, Koman, Volodymyr B., McGee, Melissa K., Olsen, Bradley D., and Strano, Michael S.

Published
2022

17. Encapsulation of the Biocontrol Agent Beauveria bassianain Cellulose Nanomaterial-Stabilized Pickering Emulsion for Foliar Application

Author

Shin, Jonghyun, Shin, Soobin, Choi, Jaeho, Jeong, Woojin, Kim, Jung-Eun, Ki, Chang Seok, Youn, Hye Jung, Son, Hokyoung, and Kwak, Seon-Yeong

Abstract

Encapsulation of living cells is a structural enhancement method by providing a viable environment to tolerate various environmental stresses such as ultraviolet (UV) radiation. The shells provided by encapsulation are constructed of additional protective layers to extend the in vitro cell lifespan. Here, we present a cellulose nanomaterial (CNM)-stabilized Pickering emulsion to encapsulate Beauveria bassianaspores. B. bassianais a widely used biocontrol agent against agricultural insect pests but is unable to survive under sunlight when applied to crop plants in the field. We synthesized Pickering emulsions stabilized by cellulose nanocrystals and cellulose nanofibrils at various ratios and analyzed the phase stability, emulsion size distribution, colloidal stability, and rheological properties. The most stable Pickering emulsion composition was then selected to encapsulate B. bassianaconidia. The encapsulated conidia maintained their viability to germinate and grow and showed excellent insecticidal activity, even after UV radiation exposure. This work demonstrated that CNM-stabilized Pickering emulsions could be utilized to encapsulate microbial cells to maintain cell viability under unfavorable environmental conditions. This finding showed the increased efficacy of B. bassianafor foliar application; this research could lead to significant advances in sustainable agriculture regarding eco-friendly pest control.

Published
2023
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20. Measuring the Accessible Surface Area within the Nanoparticle Corona using Molecular Probe Adsorption

Author

Massachusetts Institute of Technology. Department of Chemical Engineering, Singapore-MIT Alliance in Research and Technology (SMART), Park, Minkyung, Salem, Daniel P, Parviz, Dorsa, Gong, Xun, Silmore, Kevin S, Lew, Tedrick Thomas Salim, Khong, Duc Thinh, Ang, Mervin Chun-Yi, Kwak, Seon-Yeong, Chan-Park, Mary B, Strano, Michael S, Massachusetts Institute of Technology. Department of Chemical Engineering, Singapore-MIT Alliance in Research and Technology (SMART), Park, Minkyung, Salem, Daniel P, Parviz, Dorsa, Gong, Xun, Silmore, Kevin S, Lew, Tedrick Thomas Salim, Khong, Duc Thinh, Ang, Mervin Chun-Yi, Kwak, Seon-Yeong, Chan-Park, Mary B, and Strano, Michael S

Abstract

Copyright © 2019 American Chemical Society. The corona phase - the adsorbed layer of polymer, surfactant, or stabilizer molecules around a nanoparticle - is typically utilized to disperse nanoparticles into a solution or solid phase. However, this phase also controls molecular access to the nanoparticle surface, a property important for catalytic activity and sensor applications. Unfortunately, few methods can directly probe the structure of this corona phase, which is subcategorized as either a hard, immobile corona or a soft, transient corona in exchange with components in the bulk solution. In this work, we introduce a molecular probe adsorption (MPA) method for measuring the accessible nanoparticle surface area using a titration of a quenchable fluorescent molecule. For example, riboflavin is utilized to measure the surface area of gold nanoparticle standards, as well as corona phases on dispersed single-walled carbon nanotubes and graphene sheets. A material balance on the titration yields certain surface coverage parameters, including the ratio of the surface area to dissociation constant of the fluorophore, q/KD, as well as KD itself. Uncertainty, precision, and the correlation of these parameters across different experimental systems, preparations, and modalities are all discussed. Using MPA across a series of corona phases, we find that the Gibbs free energy of probe binding scales inversely with the cube root of surface area, q. In this way, MPA is the only technique to date capable of discerning critical structure-property relationships for such nanoparticle surface phases. Hence, MPA is a rapid quantitative technique that should prove useful for elucidating corona structure for nanoparticles across different systems.

Published
2021

27. Nanosensor Technology Applied to Living Plant Systems

Author

Kwak, Seon-Yeong, primary, Wong, Min Hao, additional, Lew, Tedrick Thomas Salim, additional, Bisker, Gili, additional, Lee, Michael A., additional, Kaplan, Amir, additional, Dong, Juyao, additional, Liu, Albert Tianxiang, additional, Koman, Volodymyr B., additional, Sinclair, Rosalie, additional, Hamann, Catherine, additional, and Strano, Michael S., additional

Published
2017
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33. Correction: Corrigendum: Direct Identification of On-Bead Peptides Using Surface-Enhanced Raman Spectroscopic Barcoding System for High-Throughput Bioanalysis

Author

Kang, Homan, primary, Jeong, Sinyoung, additional, Koh, Yul, additional, Geun Cha, Myeong, additional, Yang, Jin-Kyoung, additional, Kyeong, San, additional, Kim, Jaehi, additional, Kwak, Seon-Yeong, additional, Chang, Hye-Jin, additional, Lee, Hyunmi, additional, Jeong, Cheolhwan, additional, Kim, Jong-Ho, additional, Jun, Bong-Hyun, additional, Kim, Yong-Kweon, additional, Hong Jeong, Dae, additional, and Lee, Yoon-Sik, additional

Published
2015
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34. Direct Identification of On-Bead Peptides Using Surface-Enhanced Raman Spectroscopic Barcoding System for High-Throughput Bioanalysis

Author

Kang, Homan, primary, Jeong, Sinyoung, additional, Koh, Yul, additional, Geun Cha, Myeong, additional, Yang, Jin-Kyoung, additional, Kyeong, San, additional, Kim, Jaehi, additional, Kwak, Seon-Yeong, additional, Chang, Hye-Jin, additional, Lee, Hyunmi, additional, Jeong, Cheolhwan, additional, Kim, Jong-Ho, additional, Jun, Bong-Hyun, additional, Kim, Yong-Kweon, additional, Hong Jeong, Dae, additional, and Lee, Yoon-Sik, additional

Published
2015
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45. Nitroaromatic detection and infrared communication from wild-type plants using plant nanobionics

Author

Wong, Min Hao, Giraldo, Juan P., Kwak, Seon-Yeong, Koman, Volodymyr B., Sinclair, Rosalie, Lew, Tedrick Thomas Salim, Bisker, Gili, Liu, Pingwei, and Strano, Michael S.

Abstract

Plant nanobionics aims to embed non-native functions to plants by interfacing them with specifically designed nanoparticles. Here, we demonstrate that living spinach plants (Spinacia oleracea) can be engineered to serve as self-powered pre-concentrators and autosamplers of analytes in ambient groundwater and as infrared communication platforms that can send information to a smartphone. The plants employ a pair of near-infrared fluorescent nanosensors—single-walled carbon nanotubes (SWCNTs) conjugated to the peptide Bombolitin II to recognize nitroaromatics via infrared fluorescent emission, and polyvinyl-alcohol functionalized SWCNTs that act as an invariant reference signal—embedded within the plant leaf mesophyll. As contaminant nitroaromatics are transported up the roots and stem into leaf tissues, they accumulate in the mesophyll, resulting in relative changes in emission intensity. The real-time monitoring of embedded SWCNT sensors also allows residence times in the roots, stems and leaves to be estimated, calculated to be 8.3 min (combined residence times of root and stem) and 1.9 min mm−1leaf, respectively. These results demonstrate the ability of living, wild-type plants to function as chemical monitors of groundwater and communication devices to external electronics at standoff distances.

Published
2017
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48. Surface-Enhanced Raman Spectroscopic-Encoded Beads for Multiplex Immunoassay

Author

Jun, Bong-Hyun, primary, Kim, Jong-Ho, additional, Park, Hyunmi, additional, Kim, Jun-Sung, additional, Yu, Kyeong-Nam, additional, Lee, Sang-Myung, additional, Choi, Heejeong, additional, Kwak, Seon-Yeong, additional, Kim, Yong-Kweon, additional, Jeong, Dae Hong, additional, Cho, Myung-Haing, additional, and Lee, Yoon-Sik, additional

Published
2007
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50. Corrigendum: Direct Identification of On-Bead Peptides Using Surface-Enhanced Raman Spectroscopic Barcoding System for High-Throughput Bioanalysis.

Author

Kang, Homan, Jeong, Sinyoung, Koh, Yul, Geun Cha, Myeong, Yang, Jin-Kyoung, Kyeong, San, Kim, Jaehi, Kwak, Seon-Yeong, Chang, Hye-Jin, Lee, Hyunmi, Jeong, Cheolhwan, Kim, Jong-Ho, Jun, Bong-Hyun, Kim, Yong-Kweon, Hong Jeong, Dae, and Lee, Yoon-Sik

Subjects
RAMAN spectroscopy, BAR codes
Abstract

A correction to the article "Direct Identification of On-Bead Peptides Using Surface-Enhanced Raman Spectroscopic Barcoding System for High-Throughput Bioanalysis" that was published in a 2015 issue, is presented.

Published
2015
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