Search

Your search keyword '"Kvistborg, Pia"' showing total 282 results
Start Over Author "Kvistborg, Pia"
282 results on '"Kvistborg, Pia"'

1. Differential effects of PD-1 and CTLA-4 blockade on the melanoma-reactive CD8 T cell response

Author

Gangaev, Anastasia, Rozeman, Elisa A, Rohaan, Maartje W, Isaeva, Olga I, Philips, Daisy, Patiwael, Sanne, van den Berg, Joost H, Ribas, Antoni, Schadendorf, Dirk, Schilling, Bastian, Schumacher, Ton N, Blank, Christian U, Haanen, John BAG, and Kvistborg, Pia

Subjects
Biomedical and Clinical Sciences, Oncology and Carcinogenesis, Immunology, Cancer, Clinical Research, 5.1 Pharmaceuticals, Development of treatments and therapeutic interventions, Adult, Aged, Aged, 80 and over, CD8-Positive T-Lymphocytes, CTLA-4 Antigen, Cohort Studies, Epitopes, T-Lymphocyte, Female, Hepatitis A Virus Cellular Receptor 2, Humans, Immune Checkpoint Inhibitors, In Vitro Techniques, Kinetics, Lymphocyte Activation, Male, Melanoma, Melanoma-Specific Antigens, Middle Aged, Programmed Cell Death 1 Receptor, Receptors, Antigen, T-Cell, alpha-beta, Receptors, CXCR5, PD-1, CTLA-4, melanoma-reactive CD8 T cells
Abstract

Immune checkpoint inhibitors targeting programmed cell death protein 1 (PD-1) and cytotoxic T lymphocyte-associated protein 4 (CTLA-4) have revolutionized the treatment of melanoma patients. Based on early studies addressing the mechanism of action, it was assumed that PD-1 blockade mostly influences T cell responses at the tumor site. However, recent work has demonstrated that PD-1 blockade can influence the T cell compartment in peripheral blood. If the activation of circulating, tumor-reactive T cells would form an important mechanism of action of PD-1 blockade, it may be predicted that such blockade would alter either the frequency and/or the breadth of the tumor-reactive CD8 T cell response. To address this question, we analyzed CD8 T cell responses toward 71 melanoma-associated epitopes in peripheral blood of 24 melanoma patients. We show that both the frequency and the breadth of the circulating melanoma-reactive CD8 T cell response was unaltered upon PD-1 blockade. In contrast, a broadening of the circulating melanoma-reactive CD8 T cell response was observed upon CTLA-4 blockade, in concordance with our prior data. Based on these results, we conclude that PD-1 and CTLA-4 blockade have distinct mechanisms of action. In addition, the data provide an argument in favor of the hypothesis that anti-PD-1 therapy may primarily act at the tumor site.

Published
2021

2. mRNA-1273 vaccination induces polyfunctional memory CD4 and CD8 T cell responses in patients with solid cancers undergoing immunotherapy or/and chemotherapy.

Author

Gangaev, Anastasia, van Sleen, Yannick, Brandhorst, Nicole, Hoefakker, Kelly, Prajapati, Bimal, Singh, Amrita, Boerma, Annemarie, van der Heiden, Marieke, Oosting, Sjoukje F., van der Veldt, Astrid A. M., Hiltermann, T. Jeroen N., GeurtsvanKessel, Corine H., Dingemans, Anne-Marie C., Smit, Egbert F., de Vries, Elisabeth G. E., Haanen, John B. A. G., Kvistborg, Pia, and van Baarle, Debbie

Subjects
T cells, CANCER chemotherapy, COVID-19 vaccines, CANCER cells, FLOW cytometry
Abstract

Introduction: Research has confirmed the safety and comparable seroconversion rates following SARS-CoV-2 vaccination in patients with solid cancers. However, the impact of cancer treatment on vaccine-induced T cell responses remains poorly understood. Methods: In this study, we expand on previous findings within the VOICE trial by evaluating the functional and phenotypic composition of mRNA-1273-induced T cell responses in patients with solid tumors undergoing immunotherapy, chemotherapy, or both, compared to individuals without cancer. We conducted an ELISpot analysis on 386 participants to assess spike-specific T cell responses 28 days after full vaccination. Further in-depth characterization of using flow cytometry was performed on a subset of 63 participants to analyze the functional phenotype and differentiation state of spike-specific T cell responses. Results: ELISpot analysis showed robust induction of spike-specific T cell responses across all treatment groups, with response rates ranging from 75% to 80%. Flow cytometry analysis revealed a distinctive cytokine production pattern across cohorts, with CD4 T cells producing IFNγ, TNF, and IL-2, and CD8 T cells producing IFNγ, TNF, and CCL4. Variations were observed in the proportion of monofunctional CD4 T cells producing TNF, particularly higher in individuals without cancer and patients treated with chemotherapy alone, while those treated with immunotherapy or chemoimmunotherapy predominantly produced IFNγ. Despite these differences, polyfunctional spike-specific memory CD4 and CD8 T cell responses were comparable across cohorts. Notably, immunotherapy-treated patients exhibited an expansion of spike-specific CD4 T cells with a terminally differentiated effector memory phenotype. Discussion: These findings demonstrate that systemic treatment in patients with solid tumors does not compromise the quality of polyfunctional mRNA-1273-induced T cell responses. This underscores the importance of COVID-19 vaccination in patients with solid cancers undergoing systemic treatment. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

3. mRNA-1273 COVID-19 vaccination in patients receiving chemotherapy, immunotherapy, or chemoimmunotherapy for solid tumours: a prospective, multicentre, non-inferiority trial

Author

Oosting, Sjoukje F, van der Veldt, Astrid A M, GeurtsvanKessel, Corine H, Fehrmann, Rudolf S N, van Binnendijk, Rob S, Dingemans, Anne-Marie C, Smit, Egbert F, Hiltermann, T Jeroen N, den Hartog, Gerco, Jalving, Mathilda, Westphal, Tatjana T, Bhattacharya, Arkajyoti, van der Heiden, Marieke, Rimmelzwaan, Guus F, Kvistborg, Pia, Blank, Christian U, Koopmans, Marion P G, Huckriede, Anke L W, van Els, Cecile A C M, Rots, Nynke Y, van Baarle, Debbie, Haanen, John B A G, and de Vries, Elisabeth G E

Published
2021
Full Text
View/download PDF

5. Identification of bacteria-derived HLA-bound peptides in melanoma

Author

Kalaora, Shelly, Nagler, Adi, Nejman, Deborah, Alon, Michal, Barbolin, Chaya, Barnea, Eilon, Ketelaars, Steven L. C., Cheng, Kuoyuan, Vervier, Kevin, Shental, Noam, Bussi, Yuval, Rotkopf, Ron, Levy, Ronen, Benedek, Gil, Trabish, Sophie, Dadosh, Tali, Levin-Zaidman, Smadar, Geller, Leore T., Wang, Kun, Greenberg, Polina, Yagel, Gal, Peri, Aviyah, Fuks, Garold, Bhardwaj, Neerupma, Reuben, Alexandre, Hermida, Leandro, Johnson, Sarah B., Galloway-Peña, Jessica R., Shropshire, William C., Bernatchez, Chantale, Haymaker, Cara, Arora, Reetakshi, Roitman, Lior, Eilam, Raya, Weinberger, Adina, Lotan-Pompan, Maya, Lotem, Michal, Admon, Arie, Levin, Yishai, Lawley, Trevor D., Adams, David J., Levesque, Mitchell P., Besser, Michal J., Schachter, Jacob, Golani, Ofra, Segal, Eran, Geva-Zatorsky, Naama, Ruppin, Eytan, Kvistborg, Pia, Peterson, Scott N., Wargo, Jennifer A., Straussman, Ravid, and Samuels, Yardena

Published
2021
Full Text
View/download PDF

6. Preoperative ipilimumab plus nivolumab in locoregionally advanced urothelial cancer: the NABUCCO trial

Author

van Dijk, Nick, Gil-Jimenez, Alberto, Silina, Karina, Hendricksen, Kees, Smit, Laura A., de Feijter, Jeantine M., van Montfoort, Maurits L., van Rooijen, Charlotte, Peters, Dennis, Broeks, Annegien, van der Poel, Henk G., Bruining, Annemarie, Lubeck, Yoni, Sikorska, Karolina, Boellaard, Thierry N., Kvistborg, Pia, Vis, Daniel J., Hooijberg, Erik, Schumacher, Ton N., van den Broek, Maries, Wessels, Lodewyk F. A., Blank, Christian U., van Rhijn, Bas W., and van der Heijden, Michiel S.

Published
2020
Full Text
View/download PDF

7. Identification and characterization of a SARS-CoV-2 specific CD8+ T cell response with immunodominant features

Author

Gangaev, Anastasia, Ketelaars, Steven L. C., Isaeva, Olga I., Patiwael, Sanne, Dopler, Anna, Hoefakker, Kelly, De Biasi, Sara, Gibellini, Lara, Mussini, Cristina, Guaraldi, Giovanni, Girardis, Massimo, Ormeno, Cami M. P. Talavera, Hekking, Paul J. M., Lardy, Neubury M., Toebes, Mireille, Balderas, Robert, Schumacher, Ton N., Ovaa, Huib, Cossarizza, Andrea, and Kvistborg, Pia

Published
2021
Full Text
View/download PDF

8. An “alert state” ribosome population acts as a master regulator of cytokine-mediated processes

Author

Dopler, Anna, primary, Alkan, Ferhat, additional, Malka, Yuval, additional, Hoefakker, Kelly, additional, Isaeva, Olga I., additional, Kerkhoff, Mandy, additional, Gangaev, Anastasia, additional, Silva, Joana, additional, Ramalho, Sofia, additional, Hoekman, Liesbeth, additional, Altelaar, Maarten, additional, van der Kammen, Rob, additional, Yewdell, Jonathan Wilson, additional, Kvistborg, Pia, additional, and Faller, William James, additional

Published
2023
Full Text
View/download PDF

10. COVID-19 vaccination: the VOICE for patients with cancer

Author

van der Veldt, Astrid A. M., Oosting, Sjoukje F., Dingemans, Anne-Marie C., Fehrmann, Rudolf S. N., GeurtsvanKessel, Corine, Jalving, Mathilde, Rimmelzwaan, Guus F., Kvistborg, Pia, Blank, Christian U., Smit, Egbert F., Lemmens, Valery E. E. P., Hiltermann, T. Jeroen N., Koopmans, Marion P. G., Huckriede, Anke L. W., Rots, Nynke Y., van Els, Cecile A. C. M., van Baarle, Debbie, Haanen, John B. A. G., and de Vries, Elisabeth G. E.

Published
2021
Full Text
View/download PDF

11. Neoadjuvant versus adjuvant ipilimumab plus nivolumab in macroscopic stage III melanoma

Author

Blank, Christian U., Rozeman, Elisa A., Fanchi, Lorenzo F., Sikorska, Karolina, van de Wiel, Bart, Kvistborg, Pia, Krijgsman, Oscar, van den Braber, Marlous, Philips, Daisy, Broeks, Annegien, van Thienen, Johannes V., Mallo, Henk A., Adriaansz, Sandra, ter Meulen, Sylvia, Pronk, Loes M., Grijpink-Ongering, Lindsay G., Bruining, Annemarie, Gittelman, Rachel M., Warren, Sarah, van Tinteren, Harm, Peeper, Daniel S., Haanen, John B. A. G., van Akkooi, Alexander C. J., and Schumacher, Ton N.

Published
2018
Full Text
View/download PDF

12. Supplementary Figure Legends from Peripheral Blood TCR Repertoire Profiling May Facilitate Patient Stratification for Immunotherapy against Melanoma

Author

Hogan, Sabrina A., primary, Courtier, Anaïs, primary, Cheng, Phil F., primary, Jaberg-Bentele, Nicoletta F., primary, Goldinger, Simone M., primary, Manuel, Manuarii, primary, Perez, Solène, primary, Plantier, Nadia, primary, Mouret, Jean-François, primary, Nguyen-Kim, Thi Dan Linh, primary, Raaijmakers, Marieke I.G., primary, Kvistborg, Pia, primary, Pasqual, Nicolas, primary, Haanen, John B.A.G., primary, Dummer, Reinhard, primary, and Levesque, Mitchell P., primary

Published
2023
Full Text
View/download PDF

13. Figure S3 from Peripheral Blood TCR Repertoire Profiling May Facilitate Patient Stratification for Immunotherapy against Melanoma

Author

Hogan, Sabrina A., primary, Courtier, Anaïs, primary, Cheng, Phil F., primary, Jaberg-Bentele, Nicoletta F., primary, Goldinger, Simone M., primary, Manuel, Manuarii, primary, Perez, Solène, primary, Plantier, Nadia, primary, Mouret, Jean-François, primary, Nguyen-Kim, Thi Dan Linh, primary, Raaijmakers, Marieke I.G., primary, Kvistborg, Pia, primary, Pasqual, Nicolas, primary, Haanen, John B.A.G., primary, Dummer, Reinhard, primary, and Levesque, Mitchell P., primary

Published
2023
Full Text
View/download PDF

14. Data from Peripheral Blood TCR Repertoire Profiling May Facilitate Patient Stratification for Immunotherapy against Melanoma

Author

Hogan, Sabrina A., primary, Courtier, Anaïs, primary, Cheng, Phil F., primary, Jaberg-Bentele, Nicoletta F., primary, Goldinger, Simone M., primary, Manuel, Manuarii, primary, Perez, Solène, primary, Plantier, Nadia, primary, Mouret, Jean-François, primary, Nguyen-Kim, Thi Dan Linh, primary, Raaijmakers, Marieke I.G., primary, Kvistborg, Pia, primary, Pasqual, Nicolas, primary, Haanen, John B.A.G., primary, Dummer, Reinhard, primary, and Levesque, Mitchell P., primary

Published
2023
Full Text
View/download PDF

15. Data from Acquired Immune Resistance Follows Complete Tumor Regression without Loss of Target Antigens or IFNγ Signaling

Author

Donia, Marco, primary, Harbst, Katja, primary, van Buuren, Marit, primary, Kvistborg, Pia, primary, Lindberg, Mattias F., primary, Andersen, Rikke, primary, Idorn, Manja, primary, Munir Ahmad, Shamaila, primary, Ellebæk, Eva, primary, Mueller, Anja, primary, Fagone, Paolo, primary, Nicoletti, Ferdinando, primary, Libra, Massimo, primary, Lauss, Martin, primary, Hadrup, Sine Reker, primary, Schmidt, Henrik, primary, Andersen, Mads Hald, primary, thor Straten, Per, primary, Nilsson, Jonas A., primary, Schumacher, Ton N., primary, Seliger, Barbara, primary, Jönsson, Göran, primary, and Svane, Inge Marie, primary

Published
2023
Full Text
View/download PDF

16. Supplementary Figure S1-S5 from Acquired Immune Resistance Follows Complete Tumor Regression without Loss of Target Antigens or IFNγ Signaling

Author

Donia, Marco, primary, Harbst, Katja, primary, van Buuren, Marit, primary, Kvistborg, Pia, primary, Lindberg, Mattias F., primary, Andersen, Rikke, primary, Idorn, Manja, primary, Munir Ahmad, Shamaila, primary, Ellebæk, Eva, primary, Mueller, Anja, primary, Fagone, Paolo, primary, Nicoletti, Ferdinando, primary, Libra, Massimo, primary, Lauss, Martin, primary, Hadrup, Sine Reker, primary, Schmidt, Henrik, primary, Andersen, Mads Hald, primary, thor Straten, Per, primary, Nilsson, Jonas A., primary, Schumacher, Ton N., primary, Seliger, Barbara, primary, Jönsson, Göran, primary, and Svane, Inge Marie, primary

Published
2023
Full Text
View/download PDF

17. Supplementary Data from Clinical Benefit of Allogeneic Melanoma Cell Lysate–Pulsed Autologous Dendritic Cell Vaccine in MAGE-Positive Colorectal Cancer Patients

Author

Toh, Han Chong, primary, Wang, Who-Whong, primary, Chia, Whay Kuang, primary, Kvistborg, Pia, primary, Sun, Li, primary, Teo, Kelly, primary, Phoon, Yee Peng, primary, Soe, Yatanar, primary, Tan, Sze Huey, primary, Hee, Siew Wan, primary, Foo, Kian Fong, primary, Ong, Simon, primary, Koo, Wen Hsin, primary, Zocca, Mai-Britt, primary, and Claesson, Mogens H., primary

Published
2023
Full Text
View/download PDF

18. Supplementary Methods from Acquired Immune Resistance Follows Complete Tumor Regression without Loss of Target Antigens or IFNγ Signaling

Author

Donia, Marco, primary, Harbst, Katja, primary, van Buuren, Marit, primary, Kvistborg, Pia, primary, Lindberg, Mattias F., primary, Andersen, Rikke, primary, Idorn, Manja, primary, Munir Ahmad, Shamaila, primary, Ellebæk, Eva, primary, Mueller, Anja, primary, Fagone, Paolo, primary, Nicoletti, Ferdinando, primary, Libra, Massimo, primary, Lauss, Martin, primary, Hadrup, Sine Reker, primary, Schmidt, Henrik, primary, Andersen, Mads Hald, primary, thor Straten, Per, primary, Nilsson, Jonas A., primary, Schumacher, Ton N., primary, Seliger, Barbara, primary, Jönsson, Göran, primary, and Svane, Inge Marie, primary

Published
2023
Full Text
View/download PDF

21. Cancer neoantigens targeted by adoptive T cell transfer: private no more

Author

Lugli, Enrico, Kvistborg, Pia, and Galletti, Giovanni

Subjects
Cancer -- Care and treatment -- Genetic aspects, Cell proliferation -- Research, Gene mutation -- Research, T cells -- Research, Non-small cell lung cancer, Antigens, Tumors, Small cell lung cancer, Immunotherapy, Lung cancer, Cell differentiation, Immune response, Genes, Tumor proteins, Melanoma, Health care industry
Abstract

Effector T cell responses directed toward cancer neoantigens mediate tumor regression following checkpoint blockade or adoptive T cell immunotherapy, but are generally 'private', thus requiring considerable effort for their identification. In this issue of the JCI, Malekzadeh et al. show that a fraction of patients with epithelial cancers mount antigen-specific T cell responses to 'hot spot' p53 mutations that in some cases are shared among patients. This work suggests that other genes frequently mutated in human cancer can be immunogenic, thus offering a rapid way to screen for cancer neoantigens that can be targeted by subsequent T cell-based therapies., Genetic mutations of oncogenes lead to altered protein functions, resulting in increased cellular proliferation, differentiation blockade, and, ultimately, neoplastic transformation. With progression, tumors accumulate mutations and mutational load is generally [...]

Published
2019
Full Text
View/download PDF

22. HPV16 E7 DNA tattooing: safety, immunogenicity, and clinical response in patients with HPV-positive vulvar intraepithelial neoplasia

Author

Samuels, Sanne, Marijne Heeren, A., Zijlmans, Henry J. M. A. A., Welters, Marij J. P., van den Berg, Joost H., Philips, Daisy, Kvistborg, Pia, Ehsan, Ilina, Scholl, Suzy M. E., Nuijen, Bastiaan, Schumacher, Ton N. M., van Beurden, Marc, Jordanova, Ekaterina S., Haanen, John B. A. G., van der Burg, Sjoerd H., and Kenter, Gemma G.

Published
2017
Full Text
View/download PDF

23. Mutational landscape determines sensitivity to PD-1 blockade in non–small cell lung cancer

Author

Rizvi, Naiyer A., Hellmann, Matthew D., Snyder, Alexandra, Kvistborg, Pia, Makarov, Vladimir, Havel, Jonathan J., Lee, William, Yuan, Jianda, Wong, Phillip, Ho, Teresa S., Miller, Martin L., Rekhtman, Natasha, Moreira, Andre L., Ibrahim, Fawzia, Bruggeman, Cameron, Gasmi, Billel, Zappasodi, Roberta, Maeda, Yuka, Sander, Chris, Garon, Edward B., Merghoub, Taha, Wolchok, Jedd D., Schumacher, Ton N., and Chan, Timothy A.

Published
2015

25. Immunogenicity after second and third mRNA-1273 vaccination doses in patients receiving chemotherapy, immunotherapy, or both for solid tumours

Author

Oosting, Sjoukje F, primary, van der Veldt, Astrid A M, additional, Fehrmann, Rudolf S N, additional, GeurtsvanKessel, Corine H, additional, van Binnendijk, Rob S, additional, Dingemans, Anne-Marie C, additional, Smit, Egbert F, additional, Hiltermann, T Jeroen N, additional, den Hartog, Gerco, additional, Jalving, Mathilda, additional, Westphal, Tatjana T, additional, Bhattacharya, Arkajyoti, additional, de Wilt, Faye, additional, Boerma, Annemarie, additional, van Zijl, Lisanne, additional, Rimmelzwaan, Guus F, additional, Kvistborg, Pia, additional, van Els, Cecile A C M, additional, Rots, Nynke Y, additional, van Baarle, Debbie, additional, Haanen, John B A G, additional, and de Vries, Elisabeth G E, additional

Published
2022
Full Text
View/download PDF

26. IMPemBra, a phase 2 study comparing pembrolizumab with intermittent/short‐term dual MAPK pathway inhibition plus pembrolizumab in patients with melanoma harboring the BRAFV600 mutation: Three-year survival data and translational analyses.

Author

Rozeman, Elisa A., primary, Versluis, Judith M., additional, Hoefsmit, Esmée P, additional, Dimitriadis, Petros, additional, Grijpink-Ongering, Lindsay G., additional, Sikorska, Karolina, additional, van de Wiel, Bart A., additional, Heeres, Birthe C, additional, Flohil, Claudi, additional, Kvistborg, Pia, additional, van den Broek, Daan, additional, Broeks, Annegien, additional, de Groot, Jan Willem, additional, Wilgenhof, Sofie, additional, Vollebergh, Marieke A., additional, van Thienen, Johannes V., additional, Haanen, John B. A. G., additional, and Blank, Christian U., additional

Published
2022
Full Text
View/download PDF

27. In Silico Analysis Predicts a Limited Impact of SARS-CoV-2 Variants on CD8 T Cell Recognition

Author

Isaeva, Olga I., Ketelaars, Steven L. C., and Kvistborg, Pia

Subjects
fungi, Immunology, Immunology and Allergy
Abstract

Since the start of the COVID-19 pandemic, mutations have led to the emergence of new SARS-CoV-2 variants, and some of these have become prominent or dominant variants of concern. This natural course of development can have an impact on how protective the previously naturally or vaccine induced immunity is. Therefore, it is crucial to understand whether and how variant specific mutations influence host immunity. To address this, we have investigated how mutations in the recent SARS-CoV-2 variants of interest and concern influence epitope sequence similarity, predicted binding affinity to HLA, and immunogenicity of previously reported SARS-CoV-2 CD8 T cell epitopes. Our data suggests that the vast majority of SARS-CoV-2 CD8 T cell recognized epitopes are not altered by variant specific mutations. Interestingly, for the CD8 T cell epitopes that are altered due to variant specific mutations, our analyses show there is a high degree of sequence similarity between mutated and reference SARS-CoV-2 CD8 T cell epitopes. However, mutated epitopes, primarily derived from the spike protein, in SARS-CoV-2 variants Delta, AY.4.2 and Mu display reduced predicted binding affinity to their restriction element. These findings indicate that the recent SARS-CoV-2 variants of interest and concern have limited ability to escape memory CD8 T cell responses raised by vaccination or prior infection with SARS-CoV-2 early in the pandemic. The overall low impact of the mutations on CD8 T cell cross-recognition is in accordance with the notion that mutations in SARS-CoV-2 are primarily the result of receptor binding affinity and antibody selection pressures exerted on the spike protein, unrelated to T cell immunity.

Published
2022

28. Immunogenicity after second and third mRNA-1273 vaccination doses in patients receiving chemotherapy, immunotherapy, or both for solid tumours

Author

Oosting, Sjoukje F., van der Veldt, Astrid A.M., Fehrmann, Rudolf S.N., GeurtsvanKessel, Corine H., van Binnendijk, Rob S., Dingemans, Anne Marie C., Smit, Egbert F., Hiltermann, T. Jeroen N., den Hartog, Gerco, Jalving, Mathilda, Westphal, Tatjana T., Bhattacharya, Arkajyoti, de Wilt, Faye, Boerma, Annemarie, van Zijl, Lisanne, Rimmelzwaan, Guus F., Kvistborg, Pia, van Els, Cecile A.C.M., Rots, Nynke Y., van Baarle, Debbie, Haanen, John B.A.G., de Vries, Elisabeth G.E., Oosting, Sjoukje F., van der Veldt, Astrid A.M., Fehrmann, Rudolf S.N., GeurtsvanKessel, Corine H., van Binnendijk, Rob S., Dingemans, Anne Marie C., Smit, Egbert F., Hiltermann, T. Jeroen N., den Hartog, Gerco, Jalving, Mathilda, Westphal, Tatjana T., Bhattacharya, Arkajyoti, de Wilt, Faye, Boerma, Annemarie, van Zijl, Lisanne, Rimmelzwaan, Guus F., Kvistborg, Pia, van Els, Cecile A.C.M., Rots, Nynke Y., van Baarle, Debbie, Haanen, John B.A.G., and de Vries, Elisabeth G.E.

Published
2022

31. Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

Author

Cossarizza, Andrea, primary, Chang, Hyun‐Dong, additional, Radbruch, Andreas, additional, Abrignani, Sergio, additional, Addo, Richard, additional, Akdis, Mübeccel, additional, Andrä, Immanuel, additional, Andreata, Francesco, additional, Annunziato, Francesco, additional, Arranz, Eduardo, additional, Bacher, Petra, additional, Bari, Sudipto, additional, Barnaba, Vincenzo, additional, Barros‐Martins, Joana, additional, Baumjohann, Dirk, additional, Beccaria, Cristian G., additional, Bernardo, David, additional, Boardman, Dominic A., additional, Borger, Jessica, additional, Böttcher, Chotima, additional, Brockmann, Leonie, additional, Burns, Marie, additional, Busch, Dirk H., additional, Cameron, Garth, additional, Cammarata, Ilenia, additional, Cassotta, Antonino, additional, Chang, Yinshui, additional, Chirdo, Fernando Gabriel, additional, Christakou, Eleni, additional, Čičin‐Šain, Luka, additional, Cook, Laura, additional, Corbett, Alexandra J., additional, Cornelis, Rebecca, additional, Cosmi, Lorenzo, additional, Davey, Martin S., additional, De Biasi, Sara, additional, De Simone, Gabriele, additional, del Zotto, Genny, additional, Delacher, Michael, additional, Di Rosa, Francesca, additional, Di Santo, James, additional, Diefenbach, Andreas, additional, Dong, Jun, additional, Dörner, Thomas, additional, Dress, Regine J., additional, Dutertre, Charles‐Antoine, additional, Eckle, Sidonia B. G., additional, Eede, Pascale, additional, Evrard, Maximilien, additional, Falk, Christine S., additional, Feuerer, Markus, additional, Fillatreau, Simon, additional, Fiz‐Lopez, Aida, additional, Follo, Marie, additional, Foulds, Gemma A., additional, Fröbel, Julia, additional, Gagliani, Nicola, additional, Galletti, Giovanni, additional, Gangaev, Anastasia, additional, Garbi, Natalio, additional, Garrote, José Antonio, additional, Geginat, Jens, additional, Gherardin, Nicholas A., additional, Gibellini, Lara, additional, Ginhoux, Florent, additional, Godfrey, Dale I., additional, Gruarin, Paola, additional, Haftmann, Claudia, additional, Hansmann, Leo, additional, Harpur, Christopher M., additional, Hayday, Adrian C., additional, Heine, Guido, additional, Hernández, Daniela Carolina, additional, Herrmann, Martin, additional, Hoelsken, Oliver, additional, Huang, Qing, additional, Huber, Samuel, additional, Huber, Johanna E., additional, Huehn, Jochen, additional, Hundemer, Michael, additional, Hwang, William Y. K., additional, Iannacone, Matteo, additional, Ivison, Sabine M., additional, Jäck, Hans‐Martin, additional, Jani, Peter K., additional, Keller, Baerbel, additional, Kessler, Nina, additional, Ketelaars, Steven, additional, Knop, Laura, additional, Knopf, Jasmin, additional, Koay, Hui‐Fern, additional, Kobow, Katja, additional, Kriegsmann, Katharina, additional, Kristyanto, H., additional, Krueger, Andreas, additional, Kuehne, Jenny F., additional, Kunze‐Schumacher, Heike, additional, Kvistborg, Pia, additional, Kwok, Immanuel, additional, Latorre, Daniela, additional, Lenz, Daniel, additional, Levings, Megan K., additional, Lino, Andreia C., additional, Liotta, Francesco, additional, Long, Heather M., additional, Lugli, Enrico, additional, MacDonald, Katherine N., additional, Maggi, Laura, additional, Maini, Mala K., additional, Mair, Florian, additional, Manta, Calin, additional, Manz, Rudolf Armin, additional, Mashreghi, Mir‐Farzin, additional, Mazzoni, Alessio, additional, McCluskey, James, additional, Mei, Henrik E., additional, Melchers, Fritz, additional, Melzer, Susanne, additional, Mielenz, Dirk, additional, Monin, Leticia, additional, Moretta, Lorenzo, additional, Multhoff, Gabriele, additional, Muñoz, Luis Enrique, additional, Muñoz‐Ruiz, Miguel, additional, Muscate, Franziska, additional, Natalini, Ambra, additional, Neumann, Katrin, additional, Ng, Lai Guan, additional, Niedobitek, Antonia, additional, Niemz, Jana, additional, Almeida, Larissa Nogueira, additional, Notarbartolo, Samuele, additional, Ostendorf, Lennard, additional, Pallett, Laura J., additional, Patel, Amit A., additional, Percin, Gulce Itir, additional, Peruzzi, Giovanna, additional, Pinti, Marcello, additional, Pockley, A. Graham, additional, Pracht, Katharina, additional, Prinz, Immo, additional, Pujol‐Autonell, Irma, additional, Pulvirenti, Nadia, additional, Quatrini, Linda, additional, Quinn, Kylie M., additional, Radbruch, Helena, additional, Rhys, Hefin, additional, Rodrigo, Maria B., additional, Romagnani, Chiara, additional, Saggau, Carina, additional, Sakaguchi, Shimon, additional, Sallusto, Federica, additional, Sanderink, Lieke, additional, Sandrock, Inga, additional, Schauer, Christine, additional, Scheffold, Alexander, additional, Scherer, Hans U., additional, Schiemann, Matthias, additional, Schildberg, Frank A., additional, Schober, Kilian, additional, Schoen, Janina, additional, Schuh, Wolfgang, additional, Schüler, Thomas, additional, Schulz, Axel R., additional, Schulz, Sebastian, additional, Schulze, Julia, additional, Simonetti, Sonia, additional, Singh, Jeeshan, additional, Sitnik, Katarzyna M., additional, Stark, Regina, additional, Starossom, Sarah, additional, Stehle, Christina, additional, Szelinski, Franziska, additional, Tan, Leonard, additional, Tarnok, Attila, additional, Tornack, Julia, additional, Tree, Timothy I. M., additional, van Beek, Jasper J. P., additional, van de Veen, Willem, additional, van Gisbergen, Klaas, additional, Vasco, Chiara, additional, Verheyden, Nikita A., additional, von Borstel, Anouk, additional, Ward‐Hartstonge, Kirsten A., additional, Warnatz, Klaus, additional, Waskow, Claudia, additional, Wiedemann, Annika, additional, Wilharm, Anneke, additional, Wing, James, additional, Wirz, Oliver, additional, Wittner, Jens, additional, Yang, Jennie H. M., additional, and Yang, Juhao, additional

Published
2021
Full Text
View/download PDF

33. Thinking Outside the Gate: Single-Cell Assessments in Multiple Dimensions

Author

Kvistborg, Pia, Gouttefangeas, Cécile, Aghaeepour, Nima, Cazaly, Angelica, Chattopadhyay, Pratip K., Chan, Cliburn, Eckl, Judith, Finak, Greg, Hadrup, Sine Reker, Maecker, Holden T., Maurer, Dominik, Mosmann, Tim, Qiu, Peng, Scheuermann, Richard H., Welters, Marij J.P., Ferrari, Guido, Brinkman, Ryan R., and Britten, Cedrik M.

Published
2015
Full Text
View/download PDF

34. CANCER IMMUNOLOGY: Mutational landscape determines sensitivity to PD-1 blockade in non-small cell lung cancer

Author

Rizvi, Naiyer A., Hellmann, Matthew D., Snyder, Alexandra, Kvistborg, Pia, Makarov, Vladimir, Havel, Jonathan J., Lee, William, Yuan, Jianda, Wong, Phillip, Ho, Teresa S., Miller, Martin L., Rekhtman, Natasha, Moreira, Andre L., Ibrahim, Fawzia, Bruggeman, Cameron, Gasmi, Billel, Zappasodi, Roberta, Maeda, Yuka, Sander, Chris, Garon, Edward B., Merghoub, Taha, Wolchok, Jedd D., Schumacher, Ton N., and Chan, Timothy A.

Published
2015
Full Text
View/download PDF

35. High-throughput identification of antigen-specific TCRs by TCR gene capture

Author

Linnemann, Carsten, Heemskerk, Bianca, Kvistborg, Pia, Kluin, Roelof J.C., Bolotin, Dmitriy A., Chen, Xiaojing, Bresser, Kaspar, Nieuwland, Marja, Schotte, Remko, Michels, Samira, Gomez-Eerland, Raquel, Jahn, Lorenz, Hombrink, Pleun, Legrand, Nicolas, Shu, Chengyi Jenny, Mamedov, Ilgar Z., Blank, Christian U., Haanen, John B.A.G., Turchaninova, Maria A., Kerkhoven, Ron M., Spits, Hergen, Hadrup, Sine Reker, Heemskerk, Mirjam H.M., Blankenstein, Thomas, Chudakov, Dmitriy M., Bendle, Gavin M., and Schumacher, Ton N.M.

Subjects
Nucleotide sequencing -- Research, T cells -- Receptors, Antigen receptors, T cell -- Identification and classification, Gene therapy -- Methods, Viral antibodies -- Identification and classification, DNA sequencing -- Research, Cancer -- Care and treatment, Antibodies -- Identification and classification, Biological sciences, Health
Abstract

The transfer of T cell receptor (TCR) genes into patient T cells is a promising approach for the treatment of both viral infections and cancer. Although efficient methods exist to [...]

Published
2013
Full Text
View/download PDF

38. Abstract 1898: Accurate modeling of antigen processing and MHC peptide presentation using large-scale immunopeptidomes and a novel machine learning framework

Author

Pyke, Rachel Marty, primary, Mellacheruvu, Dattatreya, additional, Dea, Steven, additional, Abbott, Charles, additional, Phillips, Nick, additional, Desai, Sejal, additional, McClory, Rena, additional, Ketelaars, Steven, additional, Kvistborg, Pia, additional, West, John, additional, Chen, Richard, additional, and Boyle, Sean Michael, additional

Published
2021
Full Text
View/download PDF

39. Identification of presented SARS-CoV-2 HLA class I and HLA class II peptides using HLA peptidomics

Author

Nagler, Adi, primary, Kalaora, Shelly, additional, Barbolin, Chaya, additional, Gangaev, Anastasia, additional, Ketelaars, Steven L.C., additional, Alon, Michal, additional, Pai, Joy, additional, Benedek, Gil, additional, Yahalom-Ronen, Yfat, additional, Erez, Noam, additional, Greenberg, Polina, additional, Yagel, Gal, additional, Peri, Aviyah, additional, Levin, Yishai, additional, Satpathy, Ansuman T., additional, Bar-Haim, Erez, additional, Paran, Nir, additional, Kvistborg, Pia, additional, and Samuels, Yardena, additional

Published
2021
Full Text
View/download PDF

40. Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

Author

Rolf M. Schwiete Foundation, Associazione Italiana per la Ricerca sul Cancro, German Research Foundation, National Institutes of Health (US), European Commission, Cossarizza, Andrea, Chang, Hyun‐Dong, Radbruch, Andreas, Abrignani, Sergio, Addo, Richard, Akdis, Mübeccel, Andrä, Immanuel, Andreata, Francesco, Annunziato, Francesco, Arranz, Eduardo, Bacher, Petra, Knop, Laura, Knopf, Jasmin, Koay, Hui-Fern, Kobow, Katja, Kriegsmann, Katharina, Kristyanto, H., Krueger, Andreas, Kuehne, Jenny F., Kunze-Schumacher, Heike, Maini, Mala K., Verheyden, Nikita A., Kvistborg, Pia, Kwok, Immanuel, Latorre, Daniela, Mair, Florian, Bari, Sudipto, Manta, Calin, Armin Manz, Rudolf, Mashreghi, Mir-Farzin, Mazzoni, Alessio, McCluskey, James, Borstel, Anouk von, Mei, Henrik E., Melchers, Fritz, Melzer, Susanne, Mielenz, Dirk, Monin, Leticia, Barnaba, Vincenzo, Moretta, Lorenzo, Multhoff, Gabriele, Muñoz, Luis Enrique, Muñoz-Ruiz, Miguel, Ward-Hartstonge, Kirsten A., Muscate, Franziska, Natalini, Ambra, Neumann, Katrin, Guan N., Lai, Niedobitek, Antonia, Niemz, Jana, Barros-Martins, Joana, Nogueira Almeida, Larissa, Notarbartolo, Samuele, Ostendorf, Lennard, Warnatz, Klaus, Pallett, Laura J., Patel, Amit A., Itir Percin, Gulce, Peruzzi, Giovanna, Pinti, Marcello, Pockley, A. Graham, Pracht, Katharina, Baumjohann, Dirk, Prinz, Immo, Pujol-Autonell, Irma, Waskow, Claudia, Pulvirenti, Nadia, Quatrini, Linda, Quinn, Kylie M., Radbruch, Helena, Rhys, Hefin, Rodrigo, Maria B., Romagnani, Chiara, Saggau, Carina, Beccaria, Cristian G., Sakaguchi, Shimon, Wiedemann, Annika, Sallusto, Federica, Sanderink, Lieke, Sandrock, Inga, Schauer, Christine, Scheffold, Alexander, Scherer, Hans U., Schiemann, Matthias, Schildberg, Frank A., Schober, Kilian, Bernardo, David, Wilharm, Anneke, Schoen, Janina, Schuh, Wolfgang, Schüler, Thomas, Schulz, Axel R., Schulz, Sebastian, Schulze, Julia, Simonetti, Sonia, Singh, Jeeshan, Sitnik, Katarzyna M., Stark, Regina, Wing, James, Boardman, Dominic A., Starossom, Sarah, Stehle, Christina, Szelinski, Franziska, Tan, Leonard, Tarnok, Attila, Tornack, Julia, Tree, Timothy I. M., Van Beek, Jasper J. P., Veen, Willem van de, Wirz, Oliver, Gisbergen, Klaas van, Borger, Jessica, Vasco, Chiara, Böttcher, Chotima, Lenz, Daniel, Wittner, Jens, Yang, Jennie H. M., Yang, Juhao, Brockmann, Leonie, Burns, Marie, Busch, Dirk H., Cameron, Garth, Cammarata, Ilenia, Cassotta, Antonino, Chang, Yinshui, Levings, Megan K., Chirdo, Fernando G., Christakou, Eleni, Cicin-Sain, Luka, Cook, Laura, Corbett, Alexandra J., Cornelis, Rebecca, Cosmi, Lorenzo, Davey, Martin S., Biasi, Sara De, Simone, Gabriele De, Lino, Andreia C., Zotto, Genny del, Delacher, Michael, Rosa, Francesca Di, Santo, James Di, Diefenbach, Andreas, Dong, Jun, Dörner, Thomas, Dress, Regine J., Dutertre, Charles-Antoine, Eckle, Sidonia B. G., Liotta, Francesco, Eede, Pascale, Evrard, Maximilien, Falk, Christine S., Feuerer, Markus, Fillatreau, Simon, Fiz-López, Aida, Follo, Marie, Foulds, Gemma A., Fröbel, Julia, Gagliani, Nicola, Long, Heather M., Galletti, Giovanni, Gangaev, Anastasia, Garbi, Natalio, Garrote, José Antonio, Geginat, Jens, Gherardin, Nicholas A., Gibellini, Lara, Ginhoux, Florent, Godfrey, Dale I., Gruarin, Paola, Lugli, Enrico, Haftmann, Claudia, Hansmann, Leo, Harpur, Christopher M., Hayday, Adrian C., Heine, Guido, Hernández, Daniela Carolina, Herrmann, Martin, Hoelsken, Oliver, Huang, Qing, Huber, Samuel, MacDonald, Katherine N., Huber, Johanna E., Huehn, Jochen, Hundemer, Michael, Hwang, William Y. K., Iannacone, Matteo, Ivison, Sabine M., Jäck, Hans-Martin, Jani, Peter K., Keller, Baerbel, Kessler, Nina, Maggi, Laura, Ketelaars, Steven, Rolf M. Schwiete Foundation, Associazione Italiana per la Ricerca sul Cancro, German Research Foundation, National Institutes of Health (US), European Commission, Cossarizza, Andrea, Chang, Hyun‐Dong, Radbruch, Andreas, Abrignani, Sergio, Addo, Richard, Akdis, Mübeccel, Andrä, Immanuel, Andreata, Francesco, Annunziato, Francesco, Arranz, Eduardo, Bacher, Petra, Knop, Laura, Knopf, Jasmin, Koay, Hui-Fern, Kobow, Katja, Kriegsmann, Katharina, Kristyanto, H., Krueger, Andreas, Kuehne, Jenny F., Kunze-Schumacher, Heike, Maini, Mala K., Verheyden, Nikita A., Kvistborg, Pia, Kwok, Immanuel, Latorre, Daniela, Mair, Florian, Bari, Sudipto, Manta, Calin, Armin Manz, Rudolf, Mashreghi, Mir-Farzin, Mazzoni, Alessio, McCluskey, James, Borstel, Anouk von, Mei, Henrik E., Melchers, Fritz, Melzer, Susanne, Mielenz, Dirk, Monin, Leticia, Barnaba, Vincenzo, Moretta, Lorenzo, Multhoff, Gabriele, Muñoz, Luis Enrique, Muñoz-Ruiz, Miguel, Ward-Hartstonge, Kirsten A., Muscate, Franziska, Natalini, Ambra, Neumann, Katrin, Guan N., Lai, Niedobitek, Antonia, Niemz, Jana, Barros-Martins, Joana, Nogueira Almeida, Larissa, Notarbartolo, Samuele, Ostendorf, Lennard, Warnatz, Klaus, Pallett, Laura J., Patel, Amit A., Itir Percin, Gulce, Peruzzi, Giovanna, Pinti, Marcello, Pockley, A. Graham, Pracht, Katharina, Baumjohann, Dirk, Prinz, Immo, Pujol-Autonell, Irma, Waskow, Claudia, Pulvirenti, Nadia, Quatrini, Linda, Quinn, Kylie M., Radbruch, Helena, Rhys, Hefin, Rodrigo, Maria B., Romagnani, Chiara, Saggau, Carina, Beccaria, Cristian G., Sakaguchi, Shimon, Wiedemann, Annika, Sallusto, Federica, Sanderink, Lieke, Sandrock, Inga, Schauer, Christine, Scheffold, Alexander, Scherer, Hans U., Schiemann, Matthias, Schildberg, Frank A., Schober, Kilian, Bernardo, David, Wilharm, Anneke, Schoen, Janina, Schuh, Wolfgang, Schüler, Thomas, Schulz, Axel R., Schulz, Sebastian, Schulze, Julia, Simonetti, Sonia, Singh, Jeeshan, Sitnik, Katarzyna M., Stark, Regina, Wing, James, Boardman, Dominic A., Starossom, Sarah, Stehle, Christina, Szelinski, Franziska, Tan, Leonard, Tarnok, Attila, Tornack, Julia, Tree, Timothy I. M., Van Beek, Jasper J. P., Veen, Willem van de, Wirz, Oliver, Gisbergen, Klaas van, Borger, Jessica, Vasco, Chiara, Böttcher, Chotima, Lenz, Daniel, Wittner, Jens, Yang, Jennie H. M., Yang, Juhao, Brockmann, Leonie, Burns, Marie, Busch, Dirk H., Cameron, Garth, Cammarata, Ilenia, Cassotta, Antonino, Chang, Yinshui, Levings, Megan K., Chirdo, Fernando G., Christakou, Eleni, Cicin-Sain, Luka, Cook, Laura, Corbett, Alexandra J., Cornelis, Rebecca, Cosmi, Lorenzo, Davey, Martin S., Biasi, Sara De, Simone, Gabriele De, Lino, Andreia C., Zotto, Genny del, Delacher, Michael, Rosa, Francesca Di, Santo, James Di, Diefenbach, Andreas, Dong, Jun, Dörner, Thomas, Dress, Regine J., Dutertre, Charles-Antoine, Eckle, Sidonia B. G., Liotta, Francesco, Eede, Pascale, Evrard, Maximilien, Falk, Christine S., Feuerer, Markus, Fillatreau, Simon, Fiz-López, Aida, Follo, Marie, Foulds, Gemma A., Fröbel, Julia, Gagliani, Nicola, Long, Heather M., Galletti, Giovanni, Gangaev, Anastasia, Garbi, Natalio, Garrote, José Antonio, Geginat, Jens, Gherardin, Nicholas A., Gibellini, Lara, Ginhoux, Florent, Godfrey, Dale I., Gruarin, Paola, Lugli, Enrico, Haftmann, Claudia, Hansmann, Leo, Harpur, Christopher M., Hayday, Adrian C., Heine, Guido, Hernández, Daniela Carolina, Herrmann, Martin, Hoelsken, Oliver, Huang, Qing, Huber, Samuel, MacDonald, Katherine N., Huber, Johanna E., Huehn, Jochen, Hundemer, Michael, Hwang, William Y. K., Iannacone, Matteo, Ivison, Sabine M., Jäck, Hans-Martin, Jani, Peter K., Keller, Baerbel, Kessler, Nina, Maggi, Laura, and Ketelaars, Steven

Abstract

The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer-reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state-of-the-art handbook for basic and clinical researchers.

Published
2021

42. Key Parameters of Tumor Epitope Immunogenicity Revealed Through a Consortium Approach Improve Neoantigen Prediction

Author

Wells, Daniel K, van Buuren, Marit M, Dang, Kristen K, Hubbard-Lucey, Vanessa M, Sheehan, Kathleen CF, Campbell, Katie M, Lamb, Andrew, Ward, Jeffrey P, Sidney, John, Blazquez, Ana B, Rech, Andrew J, Zaretsky, Jesse M, Comin-Anduix, Begonya, Ng, Alphonsus HC, Chour, William, Yu, Thomas V, Rizvi, Hira, Chen, Jia M, Manning, Patrice, Steiner, Gabriela M, Doan, Xengie C, Tumor Neoantigen Selection Alliance, Merghoub, Taha, Guinney, Justin, Kolom, Adam, Selinsky, Cheryl, Ribas, Antoni, Hellmann, Matthew D, Hacohen, Nir, Sette, Alessandro, Heath, James R, Bhardwaj, Nina, Ramsdell, Fred, Schreiber, Robert D, Schumacher, Ton N, Kvistborg, Pia, and Defranoux, Nadine A

Subjects
Tumor Neoantigen Selection Alliance, Programmed Cell Death 1 Receptor, immunogenicity, Medical and Health Sciences, Cohort Studies, Vaccine Related, Epitopes, Neoplasms, Genetics, Humans, Antigens, Alleles, Cancer, TESLA, Antigen Presentation, epitope, Reproducibility of Results, immunogenomics, Biological Sciences, neoantigen, Good Health and Well Being, Neoplasm, Immunization, immunotherapy, Peptides, Developmental Biology
Abstract

Many approaches to identify therapeutically relevant neoantigens couple tumor sequencing with bioinformatic algorithms and inferred rules of tumor epitope immunogenicity. However, there are no reference data to compare these approaches, and the parameters governing tumor epitope immunogenicity remain unclear. Here, we assembled a global consortium wherein each participant predicted immunogenic epitopes from shared tumor sequencing data. 608 epitopes were subsequently assessed for Tcell binding in patient-matched samples. By integrating peptide features associated with presentation and recognition, we developed a model of tumor epitope immunogenicity that filtered out 98% of non-immunogenic peptideswith a precision above 0.70. Pipelines prioritizing model features had superior performance, and pipeline alterations leveraging them improved prediction performance. These findings were validated in an independent cohort of 310 epitopes prioritized from tumor sequencing data and assessed for Tcell binding. This data resource enables identification of parameters underlying effective anti-tumor immunity and is available to the research community.

Published
2020

43. Tumor infiltrating lymphocytes (TIL) therapy in metastatic melanoma

Author

van den Berg, Joost H, Heemskerk, Bianca, van Rooij, Nienke, Gomez-Eerland, Raquel, Michels, Samira, van Zon, Maaike, de Boer, Renate, Bakker, Noor A M, Jorritsma-Smit, Annelies, van Buuren, Marit M, Kvistborg, Pia, Spits, Hergen, Schotte, Remko, Mallo, Henk, Karger, Matthias, van der Hage, Joris A, Wouters, Michel W J M, Pronk, Loes M, Geukes Foppen, Marnix H, Blank, Christian U, Beijnen, Jos H, Nuijen, Bastiaan, Schumacher, Ton N, Haanen, John B A G, Afd Pharmacoepi & Clinical Pharmacology, Pharmacoepidemiology and Clinical Pharmacology, Experimental Immunology, AII - Cancer immunology, CCA - Cancer biology and immunology, CCA - Cancer Treatment and Quality of Life, Afd Pharmacoepi & Clinical Pharmacology, and Pharmacoepidemiology and Clinical Pharmacology

Subjects
Male, 0301 basic medicine, Oncology, lymphocytes, Cancer Research, T-Lymphocytes, medicine.medical_treatment, lymphocytes, tumor-infiltrating, 0302 clinical medicine, Immunology and Allergy, RC254-282, Clinical/Translational Cancer Immunotherapy, integumentary system, Melanoma, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, T cell reactivity, hemic and immune systems, Middle Aged, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Molecular Medicine, Female, immunotherapy, Adult, medicine.medical_specialty, Metastatic melanoma, Long term follow up, T cell, Immunology, chemical and pharmacologic phenomena, T cell response, adoptive, 03 medical and health sciences, Internal medicine, immunotherapy, adoptive, medicine, melanoma, Humans, Aged, Pharmacology, Tumor-infiltrating lymphocytes, business.industry, Immunotherapy, tumor-infiltrating, medicine.disease, 030104 developmental biology, business, Follow-Up Studies
Abstract

Treatment of metastatic melanoma with autologous tumor infiltrating lymphocytes (TILs) is currently applied in several centers. Robust and remarkably consistent overall response rates, of around 50% of treated patients, have been observed across hospitals, including a substantial fraction of durable, complete responses.PurposeExecute a phase I/II feasibility study with TIL therapy in metastatic melanoma at the Netherlands Cancer Institute, with the goal to assess feasibility and potential value of a randomized phase III trial.ExperimentalTen patients were treated with TIL therapy. Infusion products and peripheral blood samples were phenotypically characterized and neoantigen reactivity was assessed. Here, we present long-term clinical outcome and translational data on neoantigen reactivity of the T cell products.ResultsFive out of 10 patients, who were all anti-PD-1 naïve at time of treatment, showed an objective clinical response, including two patients with a complete response that are both ongoing for more than 7 years. Immune monitoring demonstrated that neoantigen-specific T cells were detectable in TIL infusion products from three out of three patients analyzed. For six out of the nine neoantigen-specific T cell responses detected in these TIL products, T cell response magnitude increased significantly in the peripheral blood compartment after therapy, and neoantigen-specific T cells were detectable for up to 3 years after TIL infusion.ConclusionThe clinical results from this study confirm the robustness of TIL therapy in metastatic melanoma and the potential role of neoantigen-specific T cell reactivity. In addition, the data from this study supported the rationale to initiate an ongoing multicenter phase III TIL trial.

Published
2020

44. Tumor infiltrating lymphocytes (TIL) therapy in metastatic melanoma: boosting of neoantigen-specific T cell reactivity and long-term follow-up

Author

van den Berg, Joost H, Heemskerk, Bianca, van Rooij, Nienke, Gomez-Eerland, Raquel, Michels, Samira, van Zon, Maaike, de Boer, Renate, Bakker, Noor A M, Jorritsma-Smit, Annelies, van Buuren, Marit M, Kvistborg, Pia, Spits, Hergen, Schotte, Remko, Mallo, Henk, Karger, Matthias, van der Hage, Joris A, Wouters, Michel W J M, Pronk, Loes M, Geukes Foppen, Marnix H, Blank, Christian U, Beijnen, Jos H, Nuijen, Bastiaan, Schumacher, Ton N, Haanen, John B A G, van den Berg, Joost H, Heemskerk, Bianca, van Rooij, Nienke, Gomez-Eerland, Raquel, Michels, Samira, van Zon, Maaike, de Boer, Renate, Bakker, Noor A M, Jorritsma-Smit, Annelies, van Buuren, Marit M, Kvistborg, Pia, Spits, Hergen, Schotte, Remko, Mallo, Henk, Karger, Matthias, van der Hage, Joris A, Wouters, Michel W J M, Pronk, Loes M, Geukes Foppen, Marnix H, Blank, Christian U, Beijnen, Jos H, Nuijen, Bastiaan, Schumacher, Ton N, and Haanen, John B A G

Abstract

Treatment of metastatic melanoma with autologous tumor infiltrating lymphocytes (TILs) is currently applied in several centers. Robust and remarkably consistent overall response rates, of around 50% of treated patients, have been observed across hospitals, including a substantial fraction of durable, complete responses.PURPOSE: Execute a phase I/II feasibility study with TIL therapy in metastatic melanoma at the Netherlands Cancer Institute, with the goal to assess feasibility and potential value of a randomized phase III trial.EXPERIMENTAL: Ten patients were treated with TIL therapy. Infusion products and peripheral blood samples were phenotypically characterized and neoantigen reactivity was assessed. Here, we present long-term clinical outcome and translational data on neoantigen reactivity of the T cell products.RESULTS: Five out of 10 patients, who were all anti-PD-1 naïve at time of treatment, showed an objective clinical response, including two patients with a complete response that are both ongoing for more than 7 years. Immune monitoring demonstrated that neoantigen-specific T cells were detectable in TIL infusion products from three out of three patients analyzed. For six out of the nine neoantigen-specific T cell responses detected in these TIL products, T cell response magnitude increased significantly in the peripheral blood compartment after therapy, and neoantigen-specific T cells were detectable for up to 3 years after TIL infusion.CONCLUSION: The clinical results from this study confirm the robustness of TIL therapy in metastatic melanoma and the potential role of neoantigen-specific T cell reactivity. In addition, the data from this study supported the rationale to initiate an ongoing multicenter phase III TIL trial.

Published
2020

45. Tumor infiltrating lymphocytes (TIL) therapy in metastatic melanoma: boosting of neoantigen-specific T cell reactivity and long-term follow-up

Author

Afd Pharmacoepi & Clinical Pharmacology, Pharmacoepidemiology and Clinical Pharmacology, van den Berg, Joost H, Heemskerk, Bianca, van Rooij, Nienke, Gomez-Eerland, Raquel, Michels, Samira, van Zon, Maaike, de Boer, Renate, Bakker, Noor A M, Jorritsma-Smit, Annelies, van Buuren, Marit M, Kvistborg, Pia, Spits, Hergen, Schotte, Remko, Mallo, Henk, Karger, Matthias, van der Hage, Joris A, Wouters, Michel W J M, Pronk, Loes M, Geukes Foppen, Marnix H, Blank, Christian U, Beijnen, Jos H, Nuijen, Bastiaan, Schumacher, Ton N, Haanen, John B A G, Afd Pharmacoepi & Clinical Pharmacology, Pharmacoepidemiology and Clinical Pharmacology, van den Berg, Joost H, Heemskerk, Bianca, van Rooij, Nienke, Gomez-Eerland, Raquel, Michels, Samira, van Zon, Maaike, de Boer, Renate, Bakker, Noor A M, Jorritsma-Smit, Annelies, van Buuren, Marit M, Kvistborg, Pia, Spits, Hergen, Schotte, Remko, Mallo, Henk, Karger, Matthias, van der Hage, Joris A, Wouters, Michel W J M, Pronk, Loes M, Geukes Foppen, Marnix H, Blank, Christian U, Beijnen, Jos H, Nuijen, Bastiaan, Schumacher, Ton N, and Haanen, John B A G

Published
2020

46. Differential effects of PD-1 and CTLA-4 blockade on the melanoma-reactive CD8 T cell response

Author

Gangaev, Anastasia, primary, Rozeman, Elisa A., additional, Rohaan, Maartje W., additional, Philips, Daisy, additional, Patiwael, Sanne, additional, van den Berg, Joost H., additional, Ribas, Antoni, additional, Schadendorf, Dirk, additional, Schilling, Bastian, additional, Schumacher, Ton N., additional, Blank, Christian U., additional, Haanen, John B.A.G., additional, and Kvistborg, Pia, additional

Published
2020
Full Text
View/download PDF

47. Stem-like CD8 T cells mediate response of adoptive cell immunotherapy against human cancer

Author

Krishna, Sri, primary, Lowery, Frank J., additional, Copeland, Amy R., additional, Bahadiroglu, Erol, additional, Mukherjee, Ratnadeep, additional, Jia, Li, additional, Anibal, James T., additional, Sachs, Abraham, additional, Adebola, Serifat O., additional, Gurusamy, Devikala, additional, Yu, Zhiya, additional, Hill, Victoria, additional, Gartner, Jared J., additional, Li, Yong F., additional, Parkhurst, Maria, additional, Paria, Biman, additional, Kvistborg, Pia, additional, Kelly, Michael C., additional, Goff, Stephanie L., additional, Altan-Bonnet, Grégoire, additional, Robbins, Paul F., additional, and Rosenberg, Steven A., additional

Published
2020
Full Text
View/download PDF

48. Comprehensive analysis of cutaneous and uveal melanoma liver metastases

Author

Hoefsmit, Esmee P, primary, Rozeman, Elisa A, additional, Van, Trieu My, additional, Dimitriadis, Petros, additional, Krijgsman, Oscar, additional, Conway, Jordan W, additional, Pires da Silva, Ines, additional, van der Wal, Jacqueline E, additional, Ketelaars, Steven L C, additional, Bresser, Kaspar, additional, Broeks, Annegien, additional, Kerkhoven, Ron M, additional, Reeves, Jason W, additional, Warren, Sarah, additional, Kvistborg, Pia, additional, Scolyer, Richard A, additional, Kapiteijn, Ellen W, additional, Peeper, Daniel S, additional, Long, Georgina V, additional, Schumacher, Ton N M, additional, and Blank, Christian U, additional

Published
2020
Full Text
View/download PDF

50. Characterization of the tumor immune microenvironment in human papillomavirus-positive and -negative head and neck squamous cell carcinomas

Author

Succaria, Farah, primary, Kvistborg, Pia, additional, Stein, Julie E., additional, Engle, Elizabeth L., additional, McMiller, Tracee L., additional, Rooper, Lisa M., additional, Thompson, Elizabeth, additional, Berger, Alan E., additional, van den Brekel, Michiel, additional, Zuur, Charlotte L., additional, Haanen, John, additional, Topalian, Suzanne L., additional, and Taube, Janis M., additional

Published
2020
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results