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This randomized comparative study was carried out to estimate efficacy and safety of the natural complex of fermented food fibers and short chain fatty acids (dietary supplement rekicen-RD®) in antibacterial therapy of 64 patients with chronic infectious prostatitis. 32 patients of the treatment group received 8 week standard antibacterial therapy for chronic prostatitis in combination with dietary supplement rekicen-RD® and 32 patients of the control group were treated only with antibacterial therapy. Short-term and long-term results were estimated after 8 weeks and 6 months follow-up, respectively. It was found, that addition of dietary supplement rekicen- RD® to antibacterial therapy resulted in statistically significant improvement of the treatment efficacy. Compared to patients of the control group, patients of the treatment group had more pronounced positive changes of all indicators of treatment efficacy (NIH-CPSI total score, quality of life, echo-structure of prostate, the number of leukocytes in prostatic secretions). Long-term (after 6 months) clinical efficacy of the combination of antibacterial therapy with dietary supplement rekicen-RD® was 96,9%, bacteriological efficacy after 8 weeks - 87,5%, after 6 months - 81%. Notably, there was 4,5-fold reduction in the rate of antibiotics adverse side effects in the treatment group patients without a single gastro-intestinal side effect.

Prevalence and character of sensitization of lymphocytes to insulin and its prognostic significance were studied in 71 patients with ischemic heart disease and 55 healthy people. Direct overt sensitization of lymphocytes to insulin was found in 14 of 71 patients (p<0.01 compared with control group). This type of reaction was associated with myocardial infarction. Concealed indirect reaction of lymphocytes to insulin was revealed at inhibition of cells with receptors to histamine or prostaglandin synthesizing cells in 13 and 19 of 71 patients, respectively (p<0.01 and p<0.05 compared with control group). Direct overt sensitization of lymphocytes to insulin and concealed indirect sensitization revealed at inhibition of functional activity of cells with receptors to histamine serve as prognostically unfavorable factors because they are associated with development of heart failure and higher rate of lethal outcome.

Bulky pincer complexes of ruthenium are capable of C-H activation and H-elimination from the pincer ligand backbone to produce mixtures of olefin and carbene products. To characterize the products and determine the mechanisms of the C-H cleavage, reactions of [RuCl(2)(p-cymene)](2) with N,N'-bis(di-tert-butylphosphino)-1,3-diaminopropane (L1) and 1,3-bis(di-tert-butylphosphinomethyl)cyclohexane (L2) were studied using a combination of X-ray crystallography, NMR spectroscopy, and DFT computational techniques. The reaction of L1 afforded a mixture of an alkylidene, a Fischer carbene, and two olefin isomers of the 16-e monohydride RuHCl[(t)Bu(2)PNHC(3)H(4)NHPBu(t)(2)] (2), whereas the reaction of L2 gave two olefin and two alkylidene isomers of 16-e RuHCl[2,6-(CH(2)PBu(t)(2))(2)C(6)H(8)] (3), all resulting from dehydrogenations of the ligand backbone of L1 and L2. The key intermediates implicated in the C-H activation reactions were identified as 14-electron paramagnetic species RuCl(PCP), where PCP = cyclometalated L1 or L2. Thus the alpha- and beta-H elimination reactions of RuCl(PCP) involved spin change and were formally spin-forbidden. Hydrogenation of 2 and 3 afforded 16-electron dihydrides RuH(2)Cl(PCP) distinguished by a large quantum exchange coupling between the hydrides.

This communication reports the preparation of a novel trans-chelating diphosphine, 1,5-bis(di-tert-butylphosphino)-2-(S)-dimethylaminopentane, that undergoes triple C-H activation in reaction with [RuCl2(p-cymene)]2 to give a chiral square-pyramidal 16-electron carbene complex of ruthenium.

To study humoral response in patients with the generalized form of pseudotuberculosis, the agglutination test, the passive hemagglutination test and the complement fixation test with antigen obtained from "Virion" (Switzerland) were used. The study of the dynamics of antibody formation to outer membrane proteins (OMP) and Yersinia pseudotuberculosis (pYV+ and pYV-) lipopolysaccharide (LPS) revealed that the synthesis of antibodies to OMP, controlled by inv gene, exceeded the synthesis of antibodies to LPS at all periods of the disease, which determined the necessity of further study of the role of OMP in pathogenesis and in the formation of humoral response. On the basis of the study of the occurrence of antigens a high diagnostic effectiveness the enzyme immunoassay for the detection of Y. pseudotuberculosis antigens, mainly at the first stage of the disease, was noted.

The error in assessment of the neutrophil phagocytic activity is 5.8%. This is attained by removal of conglomerations of phagocytosis objects and by ruling out the minimal variations in the duration of contact between the blood and foreign corpuscles and preparation of smears to be examined on the same slide. The content of up to 90% red cells does not influence the neutrophil phagocytic activity, and higher concentration reduces its level.

Pronounced correlation between the titers of hemagglutinating antibodies to different types of shigellae and other bacteria in healthy persons and in sick persons of control groups has been established. On the basis of this regularity a new approach to the use of the passive hemagglutination test in shigellosis, taking into account the ratio of antibody titers to different Shigella types, has been developed. This essentially increases the sensitivity and specificity of the test, thus making it possible to standardize its evaluation and permitting the effective diagnosis of shigellosis from the onset of the disease.

As shown in in vitro original modelling of red cell action on phagocytic process in the whole blood of patients with urological inflammation, these patients have increased count of neutrophils involved in phagocytosis, but low specific absorption of these neutrophils due to red cells. In chronic renal insufficiency characterized by a low phagocytic activity of neutrophils only stimulating potential of red cells can be noted.

Intestinal dysbacteriosis was found in all 37 patients with intestinal yersiniosis or pseudotuberculosis. Administration of biopreparations containing lacto- or bifidobacteria produced a good clinico-microbiological response rate (above 90%). The authors think it valid to include biopreparations in combined treatment of yersiniosis to prevent unfavourable outcomes.

The functional activity of peripheral blood neutrophils was studied by their main functional and metabolic parameters. The group of chronic brucellosis patients under study included 32 patients. The control group consisted of 35 practically healthy donors. The complex of methods, used for the evaluation of the functional activity of peripheral blood neutrophil leukocytes, included the determination of adhesion, activity of nitro blue tetrazolium reduction, enzymatic activity of myeloperoxidase and acid phosphatase, the level of cation protein and the production of active forms of oxygen (AFO) by these cells. Chronic brucellosis patients were found to have disturbances in the adhesive capacity of phagocytizing cells and the production of highly bactericidal AFO. These disturbances in the activity of phagocytizing cells may be one of the mechanisms contributing to incomplete phagocytosis in chronic brucellosis in humans.

Oxygen-dependent metabolism of peripheral neutrophils was compared for viral hepatitis and acute yersiniosis patients. The metabolism inhibition was seen in both the diseases with lower values for viral hepatitis. Neutrophil function was dependent on the disease gravity. Its recovery went in parallel with other clinical and laboratory signs of convalescence. A luminol-dependent chemiluminescence test is proposed for differential diagnosis of viral hepatitis with jaundices in generalized bacterial infection.

A relatively high sensitivity and specificity of indirect enzyme immunoassay using the antibody obtained from the blood of patients with P. falciparum-induced malaria in the acute period have been demonstrated. The substrate for antigen isolation is available and can be easily obtained in sufficient quantities.

The article discusses the clinical picture, diagnosis, and treatment of a rare pathologic condition of the cerebral vessels known by the Japanese name "moyamoya". Six cases with this disease among adults are described. Three patients had ischemic attacks, two young females were found to have intracerebral hematomas which were successfully evacuated. In one case the vascular anomaly became evident after a craniocerebral trauma and was manifested by subarachnoid hemorrhage and infarction in the subcortical structures.

Pneumoencephalography was performed in 35 patients with a significant diagnosis of disseminated sclerosis. Enlargement of the III ventricle was revealed in 21 patients (60%), dropsy of lateral ventricles in 16 (45,6%) and that of the IV ventricle in 13 (37,2%), atrophy of brain stem in 9 (25,4%), block of the subarachnoidal space in 11 patients (31,4%). The most pronounced brain changes were seen in patients with a cerebellar form of disseminated sclerosis and in those having severe motor disturbances, ataxia and tremor. The degree of hydrocephalus did not depend on the disease duration. A possible pathogenetic role of dropsy in disseminated sclerosis is discussed. Hydrocephalus may be considered as an additional clinicoroentgenological feature of disseminated sclerosis.

Suppressive therapy with chloroquine and fansidar at standard doses does not prevent malaria at the time of a maximum level of infecting agent transmission but only weakens the expression of clinical manifestations. The main clinical signs are nonspecific symptoms of infectious intoxication. At the seasonal peak of tropical malaria incidence, doses of individual chemoprevention should be doubled as compared to the standard ones.