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17 results on '"Kuwahara, Masakazu"'

3. Characterization of highly pathogenic avian influenza virus A(H5N6), Japan, November 2016

Author

Okamatsu, Masatoshi, Ozawa, Makoto, Soda, Kosuke, Takakuwa, Hiroki, Haga, Atsushi, Hiono, Takahiro, Matsuu, Aya, Uchida, Yuko, Iwata, Ritsuko, Matsuno, Keita, Kuwahara, Masakazu, Yabuta, Toshiyo, Usui, Tatsufumi, Ito, Hiroshi, Onuma, Manabu, Sakoda, Yoshihiro, Saito, Takehiko, Otsuki, Koichi, Ito, Toshihiro, and Kida, Hiroshi

Subjects
Avian influenza viruses -- Genetic aspects -- Research, Health
Abstract

Since their emergence in [approximately equal to] 2010-11 in China (1), highly pathogenic avian influenza viruses (HPAIVs) that have the hemagglutinin (HA) genes of the H5 subtype classified into the [...]

Published
2017
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7. Phylogenetic variations of highly pathogenic H5N6 avian influenza viruses isolated from wild birds in the Izumi plain, Japan, during the 2016–17 winter season

Author

Ozawa, Makoto, primary, Matsuu, Aya, additional, Khalil, Ahmed Magdy, additional, Nishi, Natsuko, additional, Tokorozaki, Kaori, additional, Masatani, Tatsunori, additional, Horie, Masayuki, additional, Okuya, Kosuke, additional, Ueno, Kosei, additional, Kuwahara, Masakazu, additional, and Toda, Shigehisa, additional

Published
2018
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8. Development of immersion vaccine for bacterial cold-water disease in ayu Plecoglossus altivelis

Author

Nakayama, Hitoshi, Mori, Mariko, Takita, Teisuke, Yasukawa, Kiyoshi, Tanaka, Keisuke, Hattori, Shunji, Aikawa, Hideaki, Hasegawa, Osamu, Okamura, Takashi, Takegami, Kentarou, Motokawa, Shogo, Kuwahara, Masakazu, and Amano, Kenichi

Abstract

Flavobacterium psychrophilum (F. psychrophilum) is the causative agent of bacterial cold-water disease (BCWD) that occurs in ayu Plecoglossus altivelis. Formalin-killed cell of F. psychrophilum has long been studied as an immersion vaccine for BCWD. In this study, we explored the possibility of F. psychrophilum collagenase (fpcol) for use as the immersion vaccine. BCWD convalescent ayu sera contained specific IgM antibodies against somatic F. psychrophilum and fpcol, meaning that fpcol is a promising antigen for the vaccine development. The recombinant fpcol was successfully expressed in Escherichia coli and Brevibacillus chosinensis (B. chosinensis). The culture supernatant of the B. chosinensis was used as an immersion vaccine solution. The vaccinated ayu were then challenged by soaking into F. psychrophilum culture. In two experimental groups, the relative percentages of survivals were 63 and 38%, respectively, suggesting that fpcol is promising as the immersion vaccine for ayu-BCWD.

Published
2017
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9. Characterization of Highly Pathogenic Avian Influenza Virus A(H5N6), Japan, November 2016

Author

1000000507163, Okamatsu, Masatoshi, Ozawa, Makoto, Soda, Kosuke, Takakuwa, Hiroki, Haga, Atsushi, Hiono, Takahiro, Matsuu, Aya, Uchida, Yuko, Iwata, Ritsuko, 1000040753306, Matsuno, Keita, Kuwahara, Masakazu, Yabuta, Toshiyo, Usui, Tatsufumi, Ito, Hiroshi, Onuma, Manabu, 1000040333637, Sakoda, Yoshihiro, Saito, Takehiko, Otsuki, Koichi, Ito, Toshihiro, 1000010109506, Kida, Hiroshi, 1000000507163, Okamatsu, Masatoshi, Ozawa, Makoto, Soda, Kosuke, Takakuwa, Hiroki, Haga, Atsushi, Hiono, Takahiro, Matsuu, Aya, Uchida, Yuko, Iwata, Ritsuko, 1000040753306, Matsuno, Keita, Kuwahara, Masakazu, Yabuta, Toshiyo, Usui, Tatsufumi, Ito, Hiroshi, Onuma, Manabu, 1000040333637, Sakoda, Yoshihiro, Saito, Takehiko, Otsuki, Koichi, Ito, Toshihiro, 1000010109506, and Kida, Hiroshi

Abstract

Highly pathogenic avian influenza viruses (HPAIVs) A(H5N6) were concurrently introduced into several distant regions of Japan in November 2016. These viruses were classified into the genetic clade 2.3.4.4c and were genetically closely related to H5N6 HPAIVs recently isolated in South Korea and China. In addition, these HPAIVs showed further antigenic drift.

Published
2017

10. Development of immersion vaccine for bacterial cold-water disease in ayu Plecoglossus altivelis

Author

Nakayama, Hitoshi, primary, Mori, Mariko, additional, Takita, Teisuke, additional, Yasukawa, Kiyoshi, additional, Tanaka, Keisuke, additional, Hattori, Shunji, additional, Aikawa, Hideaki, additional, Hasegawa, Osamu, additional, Okamura, Takashi, additional, Takegami, Kentarou, additional, Motokawa, Shogo, additional, Kuwahara, Masakazu, additional, and Amano, Kenichi, additional

Published
2017
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11. Phylogenetic variations of highly pathogenic H5N6 avian influenza viruses isolated from wild birds in the Izumi plain, Japan, during the 2016–17 winter season.

Author

Ozawa, Makoto, Matsuu, Aya, Khalil, Ahmed Magdy, Nishi, Natsuko, Tokorozaki, Kaori, Masatani, Tatsunori, Horie, Masayuki, Okuya, Kosuke, Ueno, Kosei, Kuwahara, Masakazu, and Toda, Shigehisa

Subjects
AVIAN influenza, AVIAN influenza A virus, BIRDS
Abstract

During the 2016–2017 winter season, we isolated 33 highly pathogenic avian influenza viruses (HPAIVs) of H5N6 subtype and three low pathogenic avian influenza viruses (LPAIVs) from debilitated or dead wild birds, duck faeces, and environmental water samples collected in the Izumi plain, an overwintering site for migratory birds in Japan. Genetic analyses of the H5N6 HPAIV isolates revealed previously unreported phylogenetic variations in the PB2, PB1, PA, and NS gene segments and allowed us to propose two novel genotypes for the contemporary H5N6 HPAIVs. In addition, analysis of the four gene segments identified close phylogenetic relationships between our three LPAIV isolates and the contemporary H5N6 HPAIV isolates. Our results implied the co‐circulation and co‐evolution of HPAIVs and LPAIVs within the same wild bird populations, thereby highlighting the importance of avian influenza surveillance targeting not only for HPAIVs but also for LPAIVs. [ABSTRACT FROM AUTHOR]

Published
2019
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15. Pathogenicity of Streptococcus iniae and Lactococcus garvieae in Farmed Thread-sail Filefish and Efficacy of the Formalin-killed Vaccines against These Bacteria

Author

Minami, Takayuki, primary, Kanemaru, Masanori, additional, Iwata, Kazuo, additional, Kuwahara, Masakazu, additional, Amano, Kenichi, additional, Mizuta, Atushi, additional, Maeda, Nobuhiro, additional, Nishiki, Issei, additional, Tue, Yuya, additional, and Yoshida, Terutoyo, additional

Published
2013
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