45 results on '"Kurucz K"'
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2. Significant range expansion of Lloviu rirus in Europe: re-emergence in 2016, Hungary
- Author
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Kemenesi, G., primary, Boldogh, S., additional, Görföl, T., additional, Bücs, S., additional, Estók, P., additional, Budinski, I., additional, Carroll, M.W., additional, Kurucz, K., additional, Zana, B., additional, Földes, F., additional, and Jakab, F., additional
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- 2019
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3. Molecular survey of zoonotic agents in rodents from an urban environment, Hungary
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Kurucz, K., primary, Madai, M., additional, Hederics, D., additional, Bali, D., additional, Kemenesi, G., additional, and Jakab, F., additional
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- 2019
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4. High diversity of replication-associated protein encoding circular viruses in guano samples of European bats
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Kemenesi, G., primary, Zana, B., additional, Kurucz, K., additional, Vlaschenko, A., additional, Kravchenko, K., additional, Budinski, I., additional, Szodoray, F., additional, Görföl, T., additional, Bányai, K., additional, and Jakab, F., additional
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- 2016
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5. First molecular identification of Dirofilaria spp. (Onchocercidae) in mosquitoes from Serbia
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Kurucz, K., primary, Kepner, A., additional, Krtinic, B., additional, Zana, B., additional, Földes, F., additional, Banyai, K., additional, Oldal, M., additional, Jakab, F., additional, and Kemenesi, G., additional
- Published
- 2016
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6. Human disturbances and predation on artificial ground nests across an urban gradient.
- Author
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Bocz, R., Szép, D., Witz, D., Ronczyk, L., Kurucz, K., and Purger, J. J.
- Subjects
ECOLOGICAL disturbances ,PREDATION ,ARTIFICIAL habitats ,BIRD nests ,URBANIZATION & the environment - Abstract
Copyright of Animal Biodiversity & Conservation is the property of Museu de Ciencies Naturals de Barcelona and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2017
7. Novel European lineages of bat astroviruses identified in Hungary
- Author
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KEMENESI, G., primary, DALLOS, B., additional, GÖRFÖL, T., additional, BOLDOGH, S., additional, ESTÓK, P., additional, KURUCZ, K., additional, OLDAL, M., additional, NÉMETH, V., additional, MADAI, M., additional, BÁNYAI, K., additional, and JAKAB, F., additional
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- 2014
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8. Compliance with the Primer and Topcoat MACT Standards of the Aerospace NESHAP at Lockheed Martin
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Kurucz, K. et al.
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- 1998
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9. Survival probabilities of first and second clutches of blackbird (Turdus merula) in an urban environment
- Author
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Kurucz Kornelia, Kallenberger Helena, Szigeti Csilla, and Purger J.J.
- Subjects
Artificial nest ,predation ,quail ,plasticine ,botanical garden ,Pecs ,Hungary ,Biology (General) ,QH301-705.5 - Abstract
The breeding success of blackbirds was investigated in April and June 2008 and 2009 in the Botanical Garden of the University of Pecs, with a total of 50 artificial nests at each of the four sessions (with 1 quail egg and 1 plasticine egg placed in every nest). In all four study periods of the two years, 2 nests (4%) were destroyed by predators. Six nests (12%, of the nests) were not discovered in either of the cases. The survival probability of artificial nests was greater in April than in June (both years), but the difference was significant only in 2008. Nests placed into a curtain of ivy (Hedera helix) on a wall were located higher up than those in bushes, yet their predation rates were quite similar. The predation values of quail vs. plasticine eggs did not differ in 2008. In the year 2009, however, significantly more quail eggs were discovered (mostly removed), than plasticine eggs. Marks that were left on plasticine eggs originated mostly from small mammals and small-bodied birds, but the disappearance of a large number of quail and plasticine eggs was probably caused by larger birds, primarily jays.
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- 2010
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10. Effects of daily nest monitoring on predation rate-an artificial nest experiment
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Kurucz, K., Frank, K., Jenő Purger, and Batáry, P.
11. Do different plasticine eggs in artificial ground nests influence nest survival
- Author
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Jenő Purger, Kurucz, K., Csuka, S., and Batáry, P.
- Subjects
Biodiversity ,Taxonomy - Abstract
Purger, J., Kurucz, K., Csuka, Sz., Bat��ry, P. (2012): Do Different Plasticine Eggs In Artificial Ground Nests Influence Nest Survival? Acta Zoologica Academiae Scientiarum Hungaricae 58 (4): 369-378, DOI: http://doi.org/10.5281/zenodo.5735981, {"references":["BALDI, A. & KISBENEDEK, T. (1999) Species-specific distribution of reed-nesting passerine birds across reed-bed edges: Effects of spatial scale and edge type. Acta Zoologica Academiae Scientiarum Hungaricae 45: 97-114.","BATARY, P. & BALDI, A. (2004) Evidence of an edge effect on avian nest success. Conservation Biology 18: 389-400.","BATARY, P. & BALDI, A. (2005) Factors affecting the survival of real and artificial Great Reed Warbler's nests. Biologia 60: 215-219.","BATES, D., MAECHLER, M. & BOLKER, B. (2011) lme4: linear mixed-effects models using S4 classes. R package version 0.999375-39. URL: http://CRAN.R-project.org/package=lme4","BAYNE, E. M. & HOBSON, K. A. (1999) Do clay eggs attract predators to artificial nest? Journal of Field Ornithology 70: 1-7.","BAYNE, E. M., HOBSON, K. A. & FARGEY, P. (1997) Predation on artificial nests in relation to forest type: Contrasting the use of quail and plasticine eggs. Ecography 20: 233-239.","BEGON, M., TOWNSEND, C. A. & HARPER, J. A. (2005) Ecology: From individuals to ecosystems. 4th ed., Wiley-Blackwell, Oxford, UK, 1068 pp.","BERRY, L. & LILL, A. (2003) Do predation rates on artificial nests accurately predict predation rates on natural nests? The effects of nest type, egg type and nest-site characteristics. Emu 103: 207-214.","BOULTON, R. L. & CASSEY, P. (2006) An inexpensive method for identifying predators of passerine nests using tethered artificial eggs. New Zealand Journal of Ecology 30: 377-385.","BOULTON, R. L. & CLARKE, M. F. (2003) Do yellow-faced honeyeater (Lichenostomus chrysops) nests experience higher predation at forest edges? Wildlife Research 30: 119-125.","BURES, S. (1997) High Common Vole Microtus arvalis predation on ground-nesting bird eggs and nestlings. Ibis 139: 173-174.","BURKE, D. M., ELLIOTT, K. A., MOORE, L., DUNFORD, W., NOEL, E., PHILLIPS, J., HOLMES, S. & FREEMARK, K. (2004) Pattern of nest predation on artificial and natural nest in forest. Conservation Biology 18: 381-388.","BUTLER, M. A. & ROTELLA, J. J. (1998) Validity of using artificial nests to assess duck-nest success. Journal of Wildlife Management 62: 163-171.","CARO, T. M. (2005) Antipredator defenses in birds and mammals. University of Chicago Press, Chicago, USA, 572 pp.","COLOMBELLI- NEGREL, D. & KLEINDORFER, S. (2009) Nest height, nest concealment, and predator type predict nest predation in superb fairy-wrens (Malurus cyaneus). Ecological Research 24: 921-928.","CRESSWELL, W. (1997) Nest predation rates and nest detectability in different stages of breeding in Blackbirds Turdus merula. Journal of Avian Biology 28: 296-302.","DAVISON, W. B. & BOLLINGER, E. (2000) Predation rates on real and artificial nests of grassland birds. Auk 117: 147-153.","DEGRAAF, R. M. & MAIER, T. J. (1996) Effect of egg size on predation by white-footed mice. Wilson Bulletin 108: 535-539.","FENSKE- CRAWFORD, T. J. & NIEMI, G. J. (1997) Predation of artificial ground nests at two types of edges in a forest-dominated landscape. Condor 99: 14-24.","FULTON, G. R. & FORD, H. A. (2003) Quail eggs, modelling clay eggs, imprints and small mammals in an Australian woodland. Emu 103: 255-258.","GOTTFRIED, B. M. & THOMPSON, C. F. (1978) Experimental-analysis of nest predation in an oldfield habitat. Auk 95: 304-312.","GREGOIRE, A., GARNIER, S., DREANO, N. & FAIVRE, B. (2003) Nest predation in Blackbirds (Turdus merula) and the influence of nest characteristics. Ornis Fennica 80: 1-10.","GUYN, K. L. & CLARK, R. G. (1997) Cover characteristics and success of natural and artificial duck nests. Journal of Field Ornithology 68: 33-41.","HASKELL, D. G. (1999) The effect of predation on begging-call evolution in nestling wood warblers. Animal Behaviour 57: 893-901.","HAZLER, K. R. (2004) Mayfield logistic regression: a practical approach for analysis of nest survival. Auk 121: 707-716.","KING, D. I., DEGRAAF, R. M., GRIFFEN, C. R. & MAIER, T. J. (1999) Do predation rates on artificial nests accurately reflect predation rates on natural bird nests? Journal of Field Ornithology 70: 257-262.","KRAUSE, J. & RUXTON, G. D. (2002) Living in groups. Oxford University Press, Oxford, UK, 210 pp.","LINDELL, C. (2000) Egg type influences predation rates in artificial nest experiments. Journal of Field Ornithology 71: 16-21.","LINDELL, C. A., COHEN, E. B. & FRITZ, J. S. (2004) Are daily mortality rates for real and artificial clutches comparable? Ornitologia Neotropical 15: 201-208.","MAIER, T. J. & DEGRAAF, R. M. (2001) Differences in depredation by small predators limit the use of plasticine and Zebra Finch eggs in artificial-nest studies. Condor 103: 180-183.","MAJOR, R. E. (1991) Identification of nest predators by photography, dummy eggs, and adhesive tape. Auk 108: 190-196.","MAJOR, R. E. & KENDAL, C. E. (1996) The contribution of artificial nest experiments to understanding avian reproductive success: a review of methods and conclusions. Ibis 138: 298-307.","MARINI, M. A., ROBINSON, S. K. & HESKE, E. J. (1995) Edge effects on nest predation in the Shawnee national forest, southern Illinois. Biological Conservation 74: 203-213.","MARTIN, T. E. (1995) Avian life-history evolution in relation to nest sites, nest predation, and food. Ecological Monographs 65: 101-127.","MOLLER, A. P. (1988) Nest predation and nest site choice in passerine birds in habitat patches of different size: a study of magpies and blackbirds. Oikos 53: 215-221.","MOORE, R. P. & ROBINSON, W. D. (2004) Artificial bird nests, external validity, and bias in ecological field studies. Ecology 85: 1562-1567.","NEWTON, I. (1998) Population limitation in birds. Academic Press, London, UK, 597 pp.","NIEHAUS, A. C., HEARD, S. B., HENDRIX, S. D. & HILLIS, S. L. (2003) Measuring edge effects on nest predation in forest fragments: Do finch and quail eggs tell different stories? American Midland Naturalist 149: 335-343.","ORTEGA, C. P., ORTEGA, J. C., RAPP, C. A. & BACKENSTO, S. A. (1998) Validating the use of artificial nests in predation experiments. Journal of Wildlife Management 62: 925-932.","PASINELLI, G. & SCHIEGG, K. (2006) Fragmentation within and between wetland reserves: the importance of spatial scales for nest predation in reed buntings. Ecography 29: 721-732.","PEHLAK, H. & LOHMUS, A. (2008) An artificial nest experiment indicates equal nesting success of waders in coastal meadows and mires. Ornis Fennica 85: 66-71.","PURGER, J. J., MESZAROS, L. A. & PURGER, D. (2004a) Predation on artificial nests in post-mining recultivated area and forest edge: contrasting the use of plasticine and quail eggs. Ecological Engineering 22: 209-212.","PURGER, J. J., MESZAROS, L. A. & PURGER, D. (2004b) Ground nesting in recultivated forest habitats - a study with artificial nests. Acta Ornithologica 39: 141-145.","PURGER, J. J., CSUKA, S. & KURUCZ, K. (2008) Predation survival of ground nesting birds in grass and wheat fields: experiment with plasticine eggs and artificial nests. Polish Journal of Ecology 56: 481-486.","PURGER, J. J., KURUCZ, K., TOTH, A. & BATARY, P. (2012) Coating plasticine eggs can eliminate the overestimation of predation on artificial ground nests. Bird Study 59: 350-352.","R DEVELOPMENT CORE TEAM (2010) R: a language and environment for statistical computing. Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051-07-0, URL: http://www.R-project.org","RANGEN, S. A., CLARC, R. G. & HOBSON, K. A. (1999) Influence of nest - site vegetation and predator community on the success of artificial songbird nests. Canadian Journal of Zoology 77: 1676-1681.","RANGEN, S. A., CLARC, R. G. & HOBSON, K. A. (2000) visual and olfactory attributes of artificial nests. Auk 117: 136-146.","ROBEL, R. J., HUGHES, J. P., KEANE, T. D. & KEMP, K. E. (2003) Do artificial nests reveal meaningful patterns of predation in Kansas grasslands? Southwestern Naturalist 48: 460-464.","SODERSTROM, B., PART, T. & RYDEN, J. (1998) Different nest predator faunas and nest predation risk on ground and shrub nests at forest ecotones: an experiment and a review. Oecologia 117: 108-118.","SVAGELJ, W. S., MERMOZ, M. E. & FERNANDEZ, G. J. (2003) Effect of egg type on the estimation of nest predation in passerines. Journal of Field Ornithology 74: 243-249.","TRNKA, A., PROKOP, P. & BATARY, P. (2008) Dummy birds in artificial nest studies: an experiment with Red-backed Shrike Lanius collurio. Bird Study 55: 329-331.","VENABLES, W. N. & RIPLEY, B. D. (2002) Modern applied statistics with S. 4th ed., Springer, New York, USA, 495 pp.","WILSON, G. R., BRITTINGHAM, M. C. & GOODRICH, L. J. (1998) How well do artificial nests estimate success of real nests? Condor 100: 357-364."]}
12. Human disturbances and predation on artificial ground nests across an urban gradient,Perturbaciones antropogénicas y depredación en nidos artificiales en el suelo en un gradiente urbano
- Author
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Renáta Bocz, Szép, D., Witz, D., Ronczyk, L., Kurucz, K., and Purger, J. J.
13. Sulfur dioxide emission control by furnace injection of a dry sorbent
- Author
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Kurucz, K
- Published
- 1985
14. The updated genome of the Hungarian population of Aedes koreicus.
- Author
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Nagy NA, Tóth GE, Kurucz K, Kemenesi G, and Laczkó L
- Subjects
- Animals, Humans, Mosquito Vectors genetics, Hungary, Europe epidemiology, Introduced Species, Aedes genetics
- Abstract
Vector-borne diseases pose a potential risk to human and animal welfare, and understanding their spread requires genomic resources. The mosquito Aedes koreicus is an emerging vector that has been introduced into Europe more than 15 years ago but only a low quality, fragmented genome was available. In this study, we carried out additional sequencing and assembled and characterized the genome of the species to provide a background for understanding its evolution and biology. The updated genome was 1.1 Gbp long and consisted of 6099 contigs with an N50 value of 329,610 bp and a BUSCO score of 84%. We identified 22,580 genes that could be functionally annotated and paid particular attention to the identification of potential insecticide resistance genes. The assessment of the orthology of the genes indicates a high turnover at the terminal branches of the species tree of mosquitoes with complete genomes, which could contribute to the adaptation and evolutionary success of the species. These results could form the basis for numerous downstream analyzes to develop targets for the control of mosquito populations., (© 2024. The Author(s).)
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- 2024
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15. Accelerating targeted mosquito control efforts through mobile West Nile virus detection.
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Varga Z, Bueno-Marí R, Risueño Iranzo J, Kurucz K, Tóth GE, Zana B, Zeghbib S, Görföl T, Jakab F, and Kemenesi G
- Subjects
- Animals, Humans, Phylogeny, Pilot Projects, Mosquito Control, Mosquito Vectors, West Nile virus genetics, West Nile Fever diagnosis, West Nile Fever prevention & control, West Nile Fever epidemiology, Culex, Culicidae
- Abstract
Background: Different mosquito control strategies have been implemented to mitigate or prevent mosquito-related public health situations. Modern mosquito control largely relies on multiple approaches, including targeted, specific treatments. Given this, it is becoming increasingly important to supplement these activities with rapid and mobile diagnostic capacities for mosquito-borne diseases. We aimed to create and test the applicability of a rapid diagnostic system for West Nile virus that can be used under field conditions., Methods: In this pilot study, various types of adult mosquito traps were applied within the regular mosquito monitoring activity framework for mosquito control. Then, the captured specimens were used for the detection of West Nile virus RNA under field conditions with a portable qRT-PCR approach within 3-4 h. Then, positive samples were subjected to confirmatory RT-PCR or NGS sequencing in the laboratory to obtain genome information of the virus. We implemented phylogenetic analysis to characterize circulating strains., Results: A total of 356 mosquito individuals representing 7 species were processed in 54 pools, each containing up to 20 individuals. These pools were tested for the presence of West Nile virus, and two pools tested positive, containing specimens from the Culex pipiens and Anopheles atroparvus mosquito species. As a result of subsequent sequencing, we present the complete genome of West Nile virus and Bagaza virus., Conclusions: The rapid identification of infected mosquitoes is the most important component of quick response adulticide or larvicide treatments to prevent human cases. The conceptual framework of real-time surveillance can be optimized for other pathogens and situations not only in relation to West Nile virus. We present an early warning system for mosquito-borne diseases and demonstrate its application to aid rapid-response mosquito control actions., (© 2024. The Author(s).)
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- 2024
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16. Correction: Using community science data to assess the association between urbanization and the presence of invasive Aedes species in Hungary.
- Author
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Garamszegi LZ, Soltész Z, Kurucz K, and Szentiványi T
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- 2023
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17. Using community science data to assess the association between urbanization and the presence of invasive Aedes species in Hungary.
- Author
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Garamszegi LZ, Soltész Z, Kurucz K, and Szentiványi T
- Subjects
- Animals, Humans, Hungary, Introduced Species, Urbanization, Ecosystem, Mosquito Vectors, Aedes
- Abstract
Background: Urbanization can be a significant contributor to the spread of invasive mosquito vector species, and the diseases they carry, as urbanized habitats provide access to a great density of food resources (humans and domestic animals) and offer abundant breeding sites for these vectors. Although anthropogenic landscapes are often associated with the presence of invasive mosquito species, we still have little understanding about the relationships between some of these and the built environment., Methods: This study explores the association between urbanization level and the occurrence of invasive Aedes species, specifically Aedes albopictus, Aedes japonicus, and Aedes koreicus, in Hungary, using data from a community (or citizen) science program undertaken between 2019 and 2022., Results: The association between each of these species and urbanized landscapes within an extensive geographic area was found to differ. Using the same standardized approach, Ae. albopictus showed a statistically significant and positive relationship with urbanization, whereas Ae. japonicus and Ae. koreicus did not., Conclusions: The findings highlight the importance of community science to mosquito research, as the data gathered using this approach can be used to make qualitative comparisons between species to explore their ecological requirements., (© 2023. The Author(s).)
- Published
- 2023
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18. Author Correction: Isolation of infectious Lloviu virus from Schreiber's bats in Hungary.
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Kemenesi G, Tóth GE, Mayora-Neto M, Scott S, Temperton N, Wright E, Mühlberger E, Hume AJ, Suder EL, Zana B, Boldogh SA, Görföl T, Estók P, Szentiványi T, Lanszki Z, Somogyi BA, Nagy Á, Pereszlényi CI, Dudás G, Földes F, Kurucz K, Madai M, Zeghbib S, Maes P, Vanmechelen B, and Jakab F
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- 2022
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19. Aedes koreicus, a vector on the rise: Pan-European genetic patterns, mitochondrial and draft genome sequencing.
- Author
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Kurucz K, Zeghbib S, Arnoldi D, Marini G, Manica M, Michelutti A, Montarsi F, Deblauwe I, Van Bortel W, Smitz N, Pfitzner WP, Czajka C, Jöst A, Kalan K, Šušnjar J, Ivović V, Kuczmog A, Lanszki Z, Tóth GE, Somogyi BA, Herczeg R, Urbán P, Bueno-Marí R, Soltész Z, and Kemenesi G
- Subjects
- Animals, Disease Vectors, Europe, Genetic Variation, Introduced Species, Mosquito Vectors genetics, Aedes genetics
- Abstract
Background: The mosquito Aedes koreicus (Edwards, 1917) is a recent invader on the European continent that was introduced to several new places since its first detection in 2008. Compared to other exotic Aedes mosquitoes with public health significance that invaded Europe during the last decades, this species' biology, behavior, and dispersal patterns were poorly investigated to date., Methodology/principal Findings: To understand the species' population relationships and dispersal patterns within Europe, a fragment of the cytochrome oxidase I (COI or COX1) gene was sequenced from 130 mosquitoes, collected from five countries where the species has been introduced and/or established. Oxford Nanopore and Illumina sequencing techniques were combined to generate the first complete nuclear and mitochondrial genomic sequences of Ae. koreicus from the European region. The complete genome of Ae. koreicus is 879 Mb. COI haplotype analyses identified five major groups (altogether 31 different haplotypes) and revealed a large-scale dispersal pattern between European Ae. koreicus populations. Continuous admixture of populations from Belgium, Italy, and Hungary was highlighted, additionally, haplotype diversity and clustering indicate a separation of German sequences from other populations, pointing to an independent introduction of Ae. koreicus to Europe. Finally, a genetic expansion signal was identified, suggesting the species might be present in more locations than currently detected., Conclusions/significance: Our results highlight the importance of genetic research of invasive mosquitoes to understand general dispersal patterns, reveal main dispersal routes and form the baseline of future mitigation actions. The first complete genomic sequence also provides a significant leap in the general understanding of this species, opening the possibility for future genome-related studies, such as the detection of 'Single Nucleotide Polymorphism' markers. Considering its public health importance, it is crucial to further investigate the species' population genetic dynamic, including a larger sampling and additional genomic markers., Competing Interests: The authors have declared that no competing interests exist.
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- 2022
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20. AIMSurv: First pan-European harmonized surveillance of Aedes invasive mosquito species of relevance for human vector-borne diseases.
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Miranda MÁ, Barceló C, Arnoldi D, Augsten X, Bakran-Lebl K, Balatsos G, Bengoa M, Bindler P, Boršová K, Bourquia M, Bravo-Barriga D, Čabanová V, Caputo B, Christou M, Delacour S, Eritja R, Fassi-Fihri O, Ferraguti M, Flacio E, Frontera E, Fuehrer HP, García-Pérez AL, Georgiades P, Gewehr S, Goiri F, González MA, Gschwind M, Gutiérrez-López R, Horváth C, Ibáñez-Justicia A, Jani V, Kadriaj P, Kalan K, Kavran M, Klobucar A, Kurucz K, Lucientes J, Lühken R, Magallanes S, Marini G, Martinou AF, Michelutti A, Mihalca AD, Montalvo T, Montarsi F, Mourelatos S, Muja-Bajraktari N, Müller P, Notarides G, Osório HC, Oteo JA, Oter K, Pajović I, Palmer JRB, Petrinic S, Răileanu C, Ries C, Rogozi E, Ruiz-Arrondo I, Sanpera-Calbet I, Sekulić N, Sevim K, Sherifi K, Silaghi C, Silva M, Sokolovska N, Soltész Z, Sulesco T, Šušnjar J, Teekema S, Valsecchi A, Vasquez MI, Velo E, Michaelakis A, Wint W, Petrić D, Schaffner F, and Della Torre A
- Abstract
Human and animal vector-borne diseases, particularly mosquito-borne diseases, are emerging or re-emerging worldwide. Six Aedes invasive mosquito (AIM) species were introduced to Europe since the 1970s: Aedes aegypti , Ae. albopictus , Ae. japonicus , Ae. koreicus , Ae. atropalpus and Ae. triseriatus . Here, we report the results of AIMSurv2020, the first pan-European surveillance effort for AIMs. Implemented by 42 volunteer teams from 24 countries. And presented in the form of a dataset named "AIMSurv Aedes Invasive Mosquito species harmonized surveillance in Europe. AIM-COST Action. Project ID: CA17108". AIMSurv2020 harmonizes field surveillance methodologies for sampling different AIMs life stages, frequency and minimum length of sampling period, and data reporting. Data include minimum requirements for sample types and recommended requirements for those teams with more resources. Data are published as a Darwin Core archive in the Global Biodiversity Information Facility- Spain, comprising a core file with 19,130 records (EventID) and an occurrences file with 19,743 records (OccurrenceID). AIM species recorded in AIMSurv2020 were Ae. albopictus , Ae. japonicus and Ae. koreicus , as well as native mosquito species., Competing Interests: The authors declare that they have no competing interests., (© The Author(s) 2022.)
- Published
- 2022
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21. Isolation of infectious Lloviu virus from Schreiber's bats in Hungary.
- Author
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Kemenesi G, Tóth GE, Mayora-Neto M, Scott S, Temperton N, Wright E, Mühlberger E, Hume AJ, Suder EL, Zana B, Boldogh SA, Görföl T, Estók P, Szentiványi T, Lanszki Z, Somogyi BA, Nagy Á, Pereszlényi CI, Dudás G, Földes F, Kurucz K, Madai M, Zeghbib S, Maes P, Vanmechelen B, and Jakab F
- Subjects
- Animals, Humans, Hungary epidemiology, Zoonoses, Chiroptera, Diptera, Filoviridae
- Abstract
Some filoviruses can be transmitted to humans by zoonotic spillover events from their natural host and filovirus outbreaks have occured with increasing frequency in the last years. The filovirus Lloviu virus (LLOV), was identified in 2002 in Schreiber's bats (Miniopterus schreibersii) in Spain and was subsequently detected in bats in Hungary. Here we isolate infectious LLOV from the blood of a live sampled Schreiber's bat in Hungary. The isolate is subsequently sequenced and cultured in the Miniopterus sp. kidney cell line SuBK12-08. It is furthermore able to infect monkey and human cells, suggesting that LLOV might have spillover potential. A multi-year surveillance of LLOV in bats in Hungary detects LLOV RNA in both deceased and live animals as well as in coupled ectoparasites from the families Nycteribiidae and Ixodidae. This correlates with LLOV seropositivity in sampled Schreiber's bats. Our data support the role of bats, specifically Miniopterus schreibersii as hosts for LLOV in Europe. We suggest that bat-associated parasites might play a role in the natural ecology of filoviruses in temperate climate regions compared to filoviruses in the tropics., (© 2022. The Author(s).)
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- 2022
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22. Effectiveness Regarding Hantavirus Detection in Rodent Tissue Samples and Urine.
- Author
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Madai M, Horváth G, Herczeg R, Somogyi B, Zana B, Földes F, Kemenesi G, Kurucz K, Papp H, Zeghbib S, and Jakab F
- Subjects
- Animals, Antibodies, Viral blood, Disease Reservoirs virology, Orthohantavirus genetics, Hungary, Kidney virology, Liver virology, Lung virology, Rodentia virology, Orthohantavirus isolation & purification, Hantavirus Infections diagnosis, Hantavirus Infections urine, Histological Techniques standards, RNA, Viral genetics
- Abstract
The natural hosts of Orthohantaviruses are rodents, soricomorphs and bats, and it is well known that they may cause serious or even fatal diseases among humans worldwide. The virus is persistent among animals and it is shed via urine, saliva and feces throughout the entirety of their lives. We aim to identify the effectiveness of hantavirus detection in rodent tissue samples and urine originating from naturally infected rodents. Initially, animals were trapped at five distinct locations throughout the Transdanubian region in Hungary. Lung, liver, kidney and urine samples were obtained from 163 deceased animals. All organs and urine were tested using nested reverse transcription polymerase chain reaction (nRT-PCR). Furthermore, sera were examined for IgG antibodies against Dobrava-Belgrade virus (DOBV) and Puumala virus (PUUV) by Western blot assay. IgG antibodies against hantaviruses and/or nucleic acid were detected in 25 (15.3%) cases. Among Apodemus, Myodes, and Microtus rodent species, DOBV, PUUV and Tula virus (TULV) were clearly identified. Amid the PCR-positive samples, the nucleic acid of the viruses was detected most effectively in the kidney (100%), while only 55% of screened lung tissues were positive. Interestingly, only three out of 20 rodent urine samples were positive when tested using nRT-PCR. Moreover, five rodents were seropositive without detectable virus nucleic acid in any of the tested organs.
- Published
- 2021
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23. Identification of Hepatitis E Virus in the Feces of Red Foxes ( Vulpes vulpes ).
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Lanszki Z, Kurucz K, Zeghbib S, Kemenesi G, Lanszki J, and Jakab F
- Abstract
Orthohepeviruses (HEV) can infect a wide range of animals, showing a relatively strict host specificity; however, its zoonotic potential, natural transmission in the wildlife are less known. Several new HEV-like viruses have been identified in various animal species, including carnivores; however, the phylogenetic relationship among these viruses is poorly resolved, since some of them were known as rodent-related so far. The red fox, the most widespread carnivore worldwide, is a known reservoir of several viruses that transmit from wildlife to humans or domestic animals; they might have a defined role in the circulation of rodent-borne HEV. In this study, we performed a HEV survey by heminested RT-PCR (Reverse Transcription PCR) on red fox fecal samples to investigate the presence of HEV in red foxes living in natural conditions, and to explore the origin of the virus via phylogenetic analysis. Out of the 26 investigated samples, HEV RNA was identified in one sample. Following Sanger sequencing, the novel sequence displayed 91% identity on the nucleotide level with recently published European common vole-HEV derived from Microtus arvalis . In contrast, it shared 85% nucleotide similarity with HEV strains described previously in red foxes. Our results strongly support "the dietary-origin" of unclassified HEV-like strains described from predators that usually prey on rodents.
- Published
- 2020
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24. Nursing homes and the elderly regarding the COVID-19 pandemic: situation report from Hungary.
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Kemenesi G, Kornya L, Tóth GE, Kurucz K, Zeghbib S, Somogyi BA, Zöldi V, Urbán P, Herczeg R, and Jakab F
- Abstract
The global impact of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is significant in terms of public health effects and its long-term socio-economic implications. Among all social groups, the elderly is by far the most affected age group regarding morbidity and mortality. In multiple countries spanning several continents, there are an increasing number of reports referencing the novel coronavirus disease-2019 (COVID-19) spread among nursing homes. These areas are now recognized as potent hotspots regarding the pandemic, which one considers with special regard. Herein, we present currently available data of fatal COVID-19 cases throughout Hungary, along with the analysis of the co-morbidity network. We also report on viral genomic data originating from a nursing home resident. The genomic data was used for viral haplotype network analysis. We emphasize the urgent need for public health authorities to focus on nursing homes and residential service units worldwide, especially in the care of the elderly and infirmed. Our results further emphasize the recent statement released by the World Health Organization (WHO) regarding the vulnerability among seniors and especially the high risk of COVID-19 emergence throughout nursing and social homes., Competing Interests: Conflicts of interestThe authors declare that they have no conflict of interest., (© The Author(s) 2020.)
- Published
- 2020
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25. Dynamics and Distribution of the Invasive Mosquito Aedes koreicus in a Temperate European City.
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Kurucz K, Manica M, Delucchi L, Kemenesi G, and Marini G
- Subjects
- Animals, Asia, Europe, Humans, Hungary, Population Density, Population Dynamics, Aedes
- Abstract
Aedes koreicus is a mosquito species native to Asia that has recently successfully invaded new areas in several European countries. Here, we provide important data on Ae. koreicus establishment in Pécs (Southern Hungary). Mosquito surveillance was carried out weekly between 2016 and 2019 at 10 different sites located throughout the city from May to September. We conducted a statistical analysis to evaluate the most important abiotic factors driving Ae. koreicus abundance. We then calibrated a previously developed temperature-dependent mathematical model to the recorded captures to evaluate mosquito abundance in the study area. We found that too high summer temperatures negatively affect mosquito abundance. The model accurately replicated the observed capture patterns, providing an estimate of Ae. koreicus density for each breeding season, which we interpolated to map Ae. koreicus abundance throughout Pécs. We found a negative correlation between mosquito captures and human density, suggesting that Ae. koreicus does not necessarily require humans for its blood meals. Our study provides a successful application of a previously published mathematical model to investigate Ae. koreicus population dynamics, proving its suitability for future studies, also within an epidemiological framework.
- Published
- 2020
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26. Genetic characterization of a novel picornavirus in Algerian bats: co-evolution analysis of bat-related picornaviruses.
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Zeghbib S, Herczeg R, Kemenesi G, Zana B, Kurucz K, Urbán P, Madai M, Földes F, Papp H, Somogyi B, and Jakab F
- Subjects
- Algeria, Animals, Host-Pathogen Interactions, Phylogeny, Picornaviridae classification, Picornaviridae isolation & purification, Chiroptera virology, Evolution, Molecular, Picornaviridae genetics
- Abstract
Bats are reservoirs of numerous zoonotic viruses. The Picornaviridae family comprises important pathogens which may infect both humans and animals. In this study, a bat-related picornavirus was detected from Algerian Minioptreus schreibersii bats for the first time in the country. Molecular analyses revealed the new virus originates to the Mischivirus genus. In the operational use of the acquired sequence and all available data regarding bat picornaviruses, we performed a co-evolutionary analysis of mischiviruses and their hosts, to authentically reveal evolutionary patterns within this genus. Based on this analysis, we enlarged the dataset, and examined the co-evolutionary history of all bat-related picornaviruses including their hosts, to effectively compile all possible species jumping events during their evolution. Furthermore, we explored the phylogeny association with geographical location, host-genus and host-species in both data sets.
- Published
- 2019
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27. Hepatitis E virus in Common voles (Microtus arvalis) from an urban environment, Hungary: Discovery of a Cricetidae-specific genotype of Orthohepevirus C.
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Kurucz K, Hederics D, Bali D, Kemenesi G, Horváth G, and Jakab F
- Subjects
- Animals, Disease Reservoirs virology, Hungary, Phylogeny, Polymerase Chain Reaction, Urban Renewal, Arvicolinae virology, Genotype, Hepatitis E virus genetics, Hepatitis E virus isolation & purification, Rodent Diseases virology
- Abstract
Hepatitis E virus is a major causative agent of acute hepatitis worldwide. Despite its zoonotic potential, there is limited information about the natural chain of hepevirus infection in wildlife, and the potential reservoir species. In this study, we performed a HEV survey by heminested RT-PCR on rodent samples from an urban environment (in the city of Pécs, Hungary) and investigated the prevalence of the virus among these native rodent species (Apodemus agrarius, Apodemus flavicollis, Apodemus sylvaticus, Microtus arvalis and Myodes glareolus). HEV was detected exclusively in Common voles (M. arvalis), in 10.2% of screened voles, and 3.2% of all investigated samples from all species. Based on the phylogenetic analysis, our strain showed the closest homology with European Orthohepevirus C strains detected previously in faecal samples of birds of prey and Red fox, supporting the possibility of the dietary origin of these strains. In addition, our samples showed close phylogenetic relation with a South American strain detected in Necromys lasiurus (Cricetidae), but separated clearly from other Muridae-associated strains, suggesting the presence of a Cricetidae-specific genotype in Europe and South-America. Based on these results, we hypothesize the reservoir role of M. arvalis rodents for the European Cricetidae-specific Orthohepevirus C genotype., (© 2018 Blackwell Verlag GmbH.)
- Published
- 2019
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28. Filarial nematode (order: Spirurida) surveillance in urban habitats, in the city of Pécs (Hungary).
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Kurucz K, Kiss V, Zana B, Jakab F, and Kemenesi G
- Subjects
- Aedes parasitology, Animals, Dirofilaria immitis classification, Dirofilaria immitis genetics, Dirofilaria immitis isolation & purification, Dirofilaria immitis physiology, Dirofilaria repens classification, Dirofilaria repens genetics, Dirofilaria repens isolation & purification, Dirofilaria repens physiology, Dirofilariasis epidemiology, Dirofilariasis transmission, Ecosystem, Female, Humans, Hungary epidemiology, Male, Mosquito Control, Mosquito Vectors classification, Mosquito Vectors physiology, Setaria Nematode classification, Setaria Nematode genetics, Setaria Nematode isolation & purification, Setaria Nematode physiology, Spirurida classification, Spirurida genetics, Spirurida physiology, Urban Health, Dirofilariasis parasitology, Mosquito Vectors parasitology, Spirurida isolation & purification
- Abstract
As part of the seasonal mosquito control activities in the city of Pécs (Baranya County, Hungary), a total of 1123 adult female mosquitoes belonging to 18 species (including the invasive species Aedes koreicus) were collected from human-inhabited areas, using CO
2 -baited traps, during two consecutive years. To survey the presence and prevalence of filarial parasites in these mosquitoes, we performed a molecular survey for filarial DNA, attempted by PCR using generic primers (COI), and followed by DNA sequencing. Filaroid nematode DNA was detected in 4% of investigated mosquito pools. Out of 410 pools, 9 pools of mosquitoes were positive for Dirofilaria repens (Aedes vexans, Aedes koreicus, Coquillettidia richiardii), and/or Dirofilaria immitis (Ae. vexans, Cq. richiardii), and further 8 pools were positive for Setaria tundra (Ae. vexans, Cq. richiardii). Our study provides novel insight for prevalence of filaroid nematodes in mosquitoes occurring in close proximity to humans, thereby highlights the possible human and veterinary health importance of these mosquito species, including the recently introduced invasive mosquito Ae. koreicus.- Published
- 2018
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29. First genetic characterization of Usutu virus from Culex pipiens mosquitoes Serbia, 2014.
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Kemenesi G, Buzás D, Zana B, Kurucz K, Krtinic B, Kepner A, Földes F, and Jakab F
- Subjects
- Animals, Mosquito Vectors virology, Phylogeny, Serbia, Culex virology, Flavivirus isolation & purification
- Abstract
Since its first appearance in Europe, Usutu virus (USUV) diverged to several different genetic lineages. The virus was reported to date from multiple countries across Europe (Hungary, Italy, Switzerland, Spain, Germany, Czech Republic and Belgium). Considering the more frequently published impact of the virus on humans it is crucial to investigate locally circulating genetic variants and trace its evolution. We retrospectively analyzed mosquito samples from Serbia Vojvodina region, collected during 2014. In this study we report the results of the screening of 23,753 female mosquitoes (753 pools) for USUV-specific nucleic-acid. Out of the 753 pools sampled, the presence of USUV RNA was confirmed in 3 pools of Culex pipiens mosquitoes, collected in August. Based on their partial NS5 sequence, all strains were identical, therefore we adjusted one representative strain for complete genome sequencing. Based on phylogenetic analysis the Serbian USUV sequences were most closely related to the virus that emerged in Austria in 2001, in Hungary in 2005 and was circulating until 2015 in Hungary. This data presents a wider geographic distribution of this genetic variant and provides the first genetic data from this region., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2018
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30. Re-emergence of Lloviu virus in Miniopterus schreibersii bats, Hungary, 2016.
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Kemenesi G, Kurucz K, Dallos B, Zana B, Földes F, Boldogh S, Görföl T, Carroll MW, and Jakab F
- Subjects
- Animals, Communicable Diseases, Emerging epidemiology, Communicable Diseases, Emerging virology, Filoviridae classification, Filoviridae genetics, Filoviridae Infections epidemiology, Filoviridae Infections pathology, Filoviridae Infections virology, Hungary epidemiology, Lung pathology, Lung virology, Phylogeny, Chiroptera virology, Communicable Diseases, Emerging veterinary, Filoviridae isolation & purification, Filoviridae Infections veterinary
- Published
- 2018
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31. Parallel Survey of Two Widespread Renal Syndrome-Causing Zoonoses: Leptospira spp. and Hantavirus in Urban Environment, Hungary.
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Kurucz K, Madai M, Bali D, Hederics D, Horváth G, Kemenesi G, and Jakab F
- Subjects
- Animals, Arvicolinae microbiology, Arvicolinae virology, Cities epidemiology, DNA, Bacterial analysis, Hantavirus Infections epidemiology, Hungary epidemiology, Leptospirosis epidemiology, Murinae microbiology, Murinae virology, RNA, Viral analysis, Rodent Diseases microbiology, Rodent Diseases virology, Zoonoses, Orthohantavirus classification, Hantavirus Infections veterinary, Leptospira classification, Leptospirosis veterinary, Rodent Diseases epidemiology
- Abstract
Rodents are important reservoir hosts for several zoonotic pathogens that cause significant morbidity and mortality in humans. Among others, leptospirosis is one of the most widespread zoonotic diseases worldwide and has the similar clinical manifestation with hantavirus infection in humans. Despite the fact that both pathogens have great epidemiological significance in Europe, no epizootiological data exist for urbanized areas so far. Therefore, this study was conducted to investigate the occurrence and prevalence of Leptospira spp. and hantaviruses in small wild rodents living in close proximity to humans. Altogether, 338 small rodents representing five different species (Apodemus agrarius, A. flavicollis, A. sylvaticus, Microtus arvalis, and Myodes glareolus) were captured in the city of Pécs (Hungary) and screened for pathogens by different types of PCR methods (TaqMan-based real-time PCR/PCR, RT-PCR/PCR). A total of 18.3% of the rodents were positive for Leptospira kirschneri, L. interrogans, and L. borgpetersenii. Nucleic acid of Tula hantavirus and human pathogen Dobrava-Belgrade orthohantavirus were detected in 8% of tested specimens. Furthermore, dual infections with both Leptospira spp. and hantaviruses were shown in 2.6% of animals, suggesting that the same rodent host can be infected with several pathogens at the same time, therefore, representing a serious threat to public health. Overall, this study provides important surveillance data on the prevalence of Leptospira spp. and hantaviruses from rodents in urbanized environment for the first time in Hungary and emphasizes the importance of further ecoepidemiological investigations.
- Published
- 2018
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32. Metagenomic analysis of bat guano samples revealed the presence of viruses potentially carried by insects, among others by Apis mellifera in Hungary.
- Author
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Zana B, Kemenesi G, Urbán P, Földes F, Görföl T, Estók P, Boldogh S, Kurucz K, and Jakab F
- Subjects
- Animals, Hungary, Insect Viruses genetics, Phylogeny, Bees virology, Chiroptera, Feces virology, Genome, Viral, Insect Viruses isolation & purification, Metagenomics
- Abstract
The predominance of dietary viruses in bat guano samples had been described recently, suggesting a new opportunity to survey the prevalence and to detect new viruses of arthropods or even plant-infecting viruses circulating locally in the ecosystem. Here we describe the diversity of viruses belonging to the order Picornavirales in Hungarian insectivorous bat guano samples. The metagenomic analysis conducted on our samples has revealed the significant predominance of aphid lethal paralysis virus (ALPV) and Big Sioux River virus (BSRV) in Hungary for the first time. Phylogenetic analysis was used to clarify the relationship to previously identified ALPV strains infecting honey bees, showing that our strain possesses a close genetic relationship with the strains that have already been described as pathogenic to honey bees. Furthermore, studies have previously confirmed the ability of these viruses to replicate in adult honey bees; however, no signs related to these viruses have been revealed yet. With the identification of two recently described possibly honey bee infecting viruses for the first time in Hungary, our results might have importance for the health conditions of Hungarian honey bee colonies in the future.
- Published
- 2018
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33. Diverse replication-associated protein encoding circular DNA viruses in guano samples of Central-Eastern European bats.
- Author
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Kemenesi G, Kurucz K, Zana B, Földes F, Urbán P, Vlaschenko A, Kravchenko K, Budinski I, Szodoray-Parádi F, Bücs S, Jére C, Csősz I, Szodoray-Parádi A, Estók P, Görföl T, Boldogh S, and Jakab F
- Subjects
- Amino Acid Sequence, Animals, Circoviridae classification, Circoviridae isolation & purification, DNA Virus Infections transmission, DNA Virus Infections virology, DNA Viruses classification, DNA Viruses isolation & purification, Europe, Eastern epidemiology, Feces virology, Georgia (Republic) epidemiology, Humans, Phylogeny, Virus Replication, Chiroptera virology, Circoviridae genetics, DNA Virus Infections epidemiology, DNA Viruses genetics, DNA, Single-Stranded genetics, DNA, Viral genetics, Genome, Viral
- Abstract
Circular replication-associated protein encoding single-stranded DNA (CRESS DNA) viruses are increasingly recognized worldwide in a variety of samples. Representative members include well-described veterinary pathogens with worldwide distribution, such as porcine circoviruses or beak and feather disease virus. In addition, numerous novel viruses belonging to the family Circoviridae with unverified pathogenic roles have been discovered in different human samples. Viruses of the family Genomoviridae have also been described as being highly abundant in different faecal and environmental samples, with case reports showing them to be suspected pathogens in human infections. In order to investigate the genetic diversity of these viruses in European bat populations, we tested guano samples from Georgia, Hungary, Romania, Serbia and Ukraine. This resulted in the detection of six novel members of the family Circoviridae and two novel members of the family Genomoviridae. Interestingly, a gemini-like virus, namely niminivirus, which was originally found in raw sewage samples in Nigeria, was also detected in our samples. We analyzed the nucleotide composition of members of the family Circoviridae to determine the possible host origins of these viruses. This study provides the first dataset on CRESS DNA viruses of European bats, and members of several novel viral species were discovered.
- Published
- 2018
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34. Non-linearities in bird responses across urbanization gradients: A meta-analysis.
- Author
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Batáry P, Kurucz K, Suarez-Rubio M, and Chamberlain DE
- Subjects
- Animals, Biodiversity, Cities, Population Dynamics, Animal Distribution, Birds physiology, Urbanization
- Abstract
Urbanization is one of the most extreme forms of environmental alteration, posing a major threat to biodiversity. We studied the effects of urbanization on avian communities via a systematic review using hierarchical and categorical meta-analyses. Altogether, we found 42 observations from 37 case studies for species richness and 23 observations from 20 case studies for abundance. Urbanization had an overall strong negative effect on bird species richness, whereas abundance increased marginally with urbanization. There was no evidence that city size played a role in influencing the relationship between urbanization and either species richness or abundance. Studies that examined long gradients (i.e. from urban to rural) were more likely to detect negative urbanization effects on species richness than studies that considered short gradients (i.e. urban vs. suburban or urban vs. rural areas). In contrast, we found little evidence that the effect of urbanization on abundance was influenced by gradient length. Effects of urbanization on species richness were more negative for studies including public green spaces (parks and other amenity areas) in the sampled landscapes. In contrast, studies performed solely in the urban matrix (i.e. no green spaces) revealed a strong positive effect on bird abundance. When performing subset analyses on urban-suburban, suburban-rural and suburban-natural comparisons, species richness decreased from natural to urban areas, but with a stronger decrease at the urban-suburban interface, whereas bird abundance showed a clear intermediate peak along the urban-rural gradient although abundance in natural areas was comparable to that in suburban areas. This suggests that species loss happens especially at the urban-suburban interface, and that the highest abundances occur in suburban areas compared to urban or rural areas. Thus, our study shows the importance of suburban areas, where the majority of birds occur with fairly high species richness., (© 2017 John Wiley & Sons Ltd.)
- Published
- 2018
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35. The former Iron Curtain still drives biodiversity-profit trade-offs in German agriculture.
- Author
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Batáry P, Gallé R, Riesch F, Fischer C, Dormann CF, Mußhoff O, Császár P, Fusaro S, Gayer C, Happe AK, Kurucz K, Molnár D, Rösch V, Wietzke A, and Tscharntke T
- Subjects
- Agriculture economics, Communism, Germany, Organic Agriculture economics, Agriculture methods, Biodiversity, Conservation of Natural Resources
- Abstract
Agricultural intensification drives biodiversity loss and shapes farmers' profit, but the role of legacy effects and detailed quantification of ecological-economic trade-offs are largely unknown. In Europe during the 1950s, the Eastern communist bloc switched to large-scale farming by forced collectivization of small farms, while the West kept small-scale private farming. Here we show that large-scale agriculture in East Germany reduced biodiversity, which has been maintained in West Germany due to >70% longer field edges than those in the East. In contrast, profit per farmland area in the East was 50% higher than that in the West, despite similar yield levels. In both regions, switching from conventional to organic farming increased biodiversity and halved yield levels, but doubled farmers' profits. In conclusion, European Union policy should acknowledge the surprisingly high biodiversity benefits of small-scale agriculture, which are on a par with conversion to organic agriculture.
- Published
- 2017
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36. Highly divergent cyclo-like virus in a great roundleaf bat (Hipposideros armiger) in Vietnam.
- Author
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Kemenesi G, Kurucz K, Zana B, Tu VT, Görföl T, Estók P, Földes F, Sztancsik K, Urbán P, Fehér E, and Jakab F
- Subjects
- Animals, Circoviridae classification, Circoviridae Infections epidemiology, Circoviridae Infections virology, DNA, Intergenic, Disease Reservoirs virology, Feces virology, Geminiviridae genetics, Phylogeny, Vietnam epidemiology, Chiroptera virology, Circoviridae genetics, Circoviridae isolation & purification, Circoviridae Infections veterinary, Genetic Variation
- Abstract
Members of the viral family Circoviridae are increasingly recognized worldwide. Bats seem to be natural reservoirs or dietary-related dispensers of these viruses. Here, we report a distantly related member of the genus Cyclovirus detected in the faeces of a great roundleaf bat (Hipposideros armiger). Interestingly, the novel virus lacks a Circoviridae-specific stem-loop structure, although a Geminiviridae-like nonamer sequence was detected in the large intergenic region. Based on these differences and its phylogenetic position, we propose that our new virus represents a distant and highly divergent member of the genus Cyclovirus. However it is lacking several characteristics of members of the genus, which raises a challenge in its taxonomic classification.
- Published
- 2017
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37. Isolation and complete genome characterization of novel reassortant orthoreovirus from common vole (Microtus arvalis).
- Author
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Fehér E, Kemenesi G, Oldal M, Kurucz K, Kugler R, Farkas SL, Marton S, Horváth G, Bányai K, and Jakab F
- Subjects
- Animals, Arvicolinae genetics, Arvicolinae virology, Orthohantavirus genetics, Hantavirus Infections virology, Humans, Molecular Sequence Data, Reassortant Viruses classification, Reassortant Viruses pathogenicity, Hantavirus Infections genetics, Phylogeny, Reassortant Viruses genetics
- Abstract
A novel mammalian orthoreovirus (MRV) strain was isolated from the lung tissue of a common vole (Microtus arvalis) with Tula hantavirus infection. Seven segments (L1-L3, M2-M3, S2, and S4) of the Hungarian MRV isolate MORV/47Ma/06 revealed a high similarity with an MRV strain detected in bank vole (Myodes glareolus) in Germany. The M1 and S3 segment of the Hungarian isolate showed the closest relationship with the sequence of a Slovenian human and a French murine isolate, respectively. The highest nucleotide and amino acid identity values were above 90 and 95% in all of the comparisons to the reference sequences in GenBank, except for the S1 with a maximum of 69.6% nucleotide and 75.4% amino acid identity. As wild rodents are among the main sources of zoonotic infections, the reservoir role of these animals and zoonotic potential of rodent origin MRVs need to be further investigated.
- Published
- 2017
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38. Candidate new rotavirus species in Schreiber's bats, Serbia.
- Author
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Bányai K, Kemenesi G, Budinski I, Földes F, Zana B, Marton S, Varga-Kugler R, Oldal M, Kurucz K, and Jakab F
- Subjects
- Animals, Disease Reservoirs, Evolution, Molecular, Feces virology, Genetic Variation, Genome, Viral, Metagenomics, Phylogeny, Rotavirus isolation & purification, Sequence Analysis, DNA, Serbia, Viral Proteins genetics, Chiroptera virology, Rotavirus genetics
- Abstract
The genus Rotavirus comprises eight species designated A to H and one tentative species, Rotavirus I. In a virus metagenomic analysis of Schreiber's bats sampled in Serbia in 2014 we obtained sequences likely representing novel rotavirus species. Whole genome sequencing and phylogenetic analysis classified the representative strain into a tentative tenth rotavirus species, we provisionally called Rotavirus J. The novel virus shared a maximum of 50% amino acid sequence identity within the VP6 gene to currently known members of the genus. This study extends our understanding of the genetic diversity of rotaviruses in bats., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2017
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39. Emergence of Aedes koreicus (Diptera: Culicidae) in an urban area, Hungary, 2016.
- Author
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Kurucz K, Kiss V, Zana B, Schmieder V, Kepner A, Jakab F, and Kemenesi G
- Subjects
- Animals, Female, Hungary, Aedes, Introduced Species
- Abstract
In June 2016, three adult females of Aedes koreicus mosquitoes were trapped in the urban area of Pécs, Southwest Hungary. The introduction of this invasive mosquito species in this region, along with the recent detection in Germany, may indicate the capability of the species to spread across Europe. Along with Aedes albopictus and Aedes japonicus mosquitoes, this is the third invasive mosquito species occurred in Hungary.
- Published
- 2016
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40. First molecular identification of Dirofilaria spp. (Onchocercidae) in mosquitoes from Serbia.
- Author
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Kurucz K, Kepner A, Krtinic B, Zana B, Földes F, Bányai K, Oldal M, Jakab F, and Kemenesi G
- Subjects
- Aedes physiology, Animals, Breeding, Culex physiology, Dirofilaria immitis classification, Dirofilaria immitis genetics, Dirofilaria repens classification, Dirofilaria repens genetics, Europe, Female, Mosquito Vectors physiology, Serbia, Aedes parasitology, Culex parasitology, Dirofilaria immitis isolation & purification, Dirofilaria repens isolation & purification, Mosquito Vectors parasitology
- Abstract
Dirofilariosis is a common and widespread veterinary health issue in several European countries with notable zoonotic potential. The causative agents are Dirofilaria immitis and Dirofilaria repens nematoda species which are transmitted by different mosquito vectors. Similar to other mosquito-borne infections, the knowledge about mosquito species involved in disease transmission is crucial for the complex understanding of local transmission cycles. Since there is no available data on mosquito species, potentially involved in disease transmission from Serbia, 6369 female mosquito individuals were retrospectively tested for Dirofilaria nematodes, collected from 13 localities in Vojvodina province, Serbia, in 2013. Altogether, 8.33 % of tested pools showed positivity, composed of five mosquito species, mainly, Culex pipiens and Aedes vexans. D. immitis and D. repens were both detected from multiple localities, during the whole period of mosquito breeding season, which provides the first data on local transmission characteristics regarding mosquitoes from the Balkans.
- Published
- 2016
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41. Genetic Characterization of Providence Virus Isolated from Bat Guano in Hungary.
- Author
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Kemenesi G, Földes F, Zana B, Kurucz K, Estók P, Boldogh S, Görföl T, Bányai K, Oldal M, and Jakab F
- Abstract
We report the complete genome sequence and genetic characterization of a novel strain of Providence virus, detected in Barbastella barbastellus bat guano, collected in Hungary in 2014. Our data may facilitate the understanding of the evolutionary processes of this unique viral family of Carmotetraviridae., (Copyright © 2016 Kemenesi et al.)
- Published
- 2016
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42. Circulation of Dirofilaria repens, Setaria tundra, and Onchocercidae species in Hungary during the period 2011-2013.
- Author
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Kemenesi G, Kurucz K, Kepner A, Dallos B, Oldal M, Herczeg R, Vajdovics P, Bányai K, and Jakab F
- Subjects
- Animals, Dirofilaria repens genetics, Female, Humans, Hungary epidemiology, Insect Vectors parasitology, Phylogeny, Time Factors, Culicidae parasitology, Dirofilaria repens isolation & purification, Nematoda isolation & purification
- Abstract
Dirofilaria repens and recently Dirofilaria immitis are known to be endemic in Hungary. Since there is no related research on Dirofilaria carrier mosquito species from Hungary, we conducted a three-year mosquito surveillance study between 2011 and 2013. During the study period we examined 23,139 female mosquitoes with a generic filaria-specific TaqMan PCR assay, and characterized them by sequencing a 500 bp segment of 12S rRNA. An important result of our study was the detection of Setaria tundra and D. repens along with an unidentified Onchocercidae nematode. D. repens is known to be endemic in Hungary, however, the detection of S. tundra in all sampling sites throughout the study period indicates for the first time the endemicity of this parasite in Hungary. The Onchocercidae sp. nematode showed 95% nucleotide identity with previously detected unidentified nematodes from Germany, indicating a broader geographical distribution of this nematode in Europe. D. immitis specific DNA was not detected among the screened mosquitoes in this study. Here we report 11 mosquito species as potential vector organisms for local filarial infections, including Aedes vexans, Ochlerotatus annulipes, Ochlerotatus sticticus, Coquillettidia richiardii, Anopheles hyrcanus and Ochlerotatus rusticus. Dirofilaria development unit was calculated and the potential transmission period was estimated, which ranged between 65 and 113 days between sampling seasons. A relatively high infection rate (36.8%) was identified, which is a notable finding for veterinary and human health professionals. Moreover, the results of our study widen the group of possible mosquito vector species for D. repens and S. tundra in Central Europe., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
43. Genetic diversity and recombination within bufaviruses: Detection of a novel strain in Hungarian bats.
- Author
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Kemenesi G, Dallos B, Görföl T, Estók P, Boldogh S, Kurucz K, Oldal M, Marton S, Bányai K, and Jakab F
- Subjects
- Amino Acid Motifs, Animals, Bunyaviridae classification, Bunyaviridae isolation & purification, Chiroptera virology, Disease Reservoirs virology, Genome, Viral, Metagenomics, Phylogeny, Viral Proteins chemistry, Viral Proteins genetics, Zoonoses, Bunyaviridae genetics, Genetic Variation, Recombination, Genetic
- Abstract
Bats are important hosts of many viruses and in several cases they may serve as natural reservoirs even for viruses with zoonotic potential worldwide, including Europe. However, they also serve as natural reservoir for other virus groups with important evolutionary relevance and yet unknown zoonotic potential. We performed viral metagenomic analyses on Miniopterus schreibersii bat fecal samples. As a result, a novel parvovirus was detected for the first time in European bats. Although, bufavirus was recently discovered as a novel human infecting parvovirus, here we report sequence data of the first bufavirus from European bats related to human bufaviruses. Based on our sequence data a possible intragenic recombination event was detected within bufaviruses which may serves as an important milestone in their evolution., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
44. Genetic characterization of a novel picornavirus detected in Miniopterus schreibersii bats.
- Author
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Kemenesi G, Zhang D, Marton S, Dallos B, Görföl T, Estók P, Boldogh S, Kurucz K, Oldal M, Kutas A, Bányai K, and Jakab F
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Genome, Viral, Hungary, Metagenomics methods, Molecular Sequence Data, Phylogeny, Chiroptera virology, Picornaviridae genetics
- Abstract
Bats are important reservoirs of many viruses with zoonotic potential worldwide, including Europe. Among bat viruses, members of the Picornaviridae family remain a neglected group. We performed viral metagenomic analyses on Miniopterus schreibersii bat faecal samples, collected in Hungary in 2013. In the present study we report the first molecular data and genomic characterization of a novel picornavirus from the bat species M. schreibersii in Europe. Based on phylogenetic analyses, the novel bat picornaviruses unambiguously belong to the Mischivirus genus and were highly divergent from other bat-derived picornaviruses of the Sapelovirus genus. Although the Hungarian viruses were most closely related to Mischivirus A, they formed a separate monophyletic branch within the genus., (© 2015 The Authors.)
- Published
- 2015
- Full Text
- View/download PDF
45. Molecular survey of RNA viruses in Hungarian bats: discovering novel astroviruses, coronaviruses, and caliciviruses.
- Author
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Kemenesi G, Dallos B, Görföl T, Boldogh S, Estók P, Kurucz K, Kutas A, Földes F, Oldal M, Németh V, Martella V, Bányai K, and Jakab F
- Subjects
- Animals, Astroviridae classification, Astroviridae genetics, Astroviridae isolation & purification, Base Sequence, Caliciviridae classification, Caliciviridae genetics, Caliciviridae isolation & purification, Coronavirus classification, Coronavirus genetics, Coronavirus isolation & purification, Hungary epidemiology, Phylogeny, Prevalence, RNA Virus Infections epidemiology, RNA Virus Infections virology, RNA Viruses isolation & purification, Real-Time Polymerase Chain Reaction, Chiroptera virology, RNA Virus Infections veterinary, RNA Viruses classification, RNA Viruses genetics
- Abstract
Unlabelled: Background: Bat-borne viruses pose a potential risk to human health and are the focus of increasing scientific interest. To start gaining information about bat-transmitted viruses in Hungary, we tested multiple bat species for several virus groups between 2012 and 2013., Materials and Methods: Fecal samples were collected from bats across Hungary. We performed group-specific RT-PCR screening for astro-, calici-, corona-, lyssa-, othoreo-, paramyxo-, and rotaviruses. Positive samples were selected and sequenced for further phylogenetic analyses., Results: A total of 447 fecal samples, representing 24 European bat species were tested. Novel strains of astroviruses, coronaviruses, and caliciviruses were detected and analyzed phylogenetically. Out of the 447 tested samples, 40 (9%) bats were positive for at least one virus. Bat-transmitted astroviruses (BtAstV) were detected in eight species with a 6.93% detection rate (95% confidence interval [CI] 4.854, 9.571). Coronaviruses (BtCoV) were detected in seven bat species with a detection rate of 1.79% (95% CI 0.849, 3.348), whereas novel caliciviruses (BtCalV) were detected in three bat species with a detection rate of 0.67% (95% CI 0.189, 1.780). Phylogenetic analyses revealed a great diversity among astrovirus strains, whereas the Hungarian BtCoV strains clustered together with both alpha- and betacoronavirus strains from other European countries. One of the most intriguing findings of our investigation is the discovery of novel BtCalVs in Europe. The Hungarian BtCalV did not cluster with any of the calcivirus genera identified in the family so far., Conclusions: We have successfully confirmed BtCoVs in numerous bat species. Furthermore, we have described new bat species harboring BtAstVs in Europe and found new species of CalVs. Further long-term investigations involving more species are needed in the Central European region for a better understanding on the host specificity, seasonality, phylogenetic relationships, and the possible zoonotic potential of these newly described viruses.
- Published
- 2014
- Full Text
- View/download PDF
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