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Diabetes increases in the worldwide by prevalence and numbers of complications related to it. The most common risk factor for foot ulcers in diabetic is peripheral neuropathy. In diabetic condition, Ca2+ concentration intracellular increases. NRF2 (Nuclear Factor Erythroid 2) acts as a bridging link in various inflammatory and apoptotic pathways impacting progress of diabetic neuropathy. The aim of this study is to predict the pathway of NRF2 activation that is mediated by the increased of intracellular Ca2+. Data interaction between Ca2+ and NRF2 were retrieved from STITCH (Search Tool for Interacting Chemicals) which were experimentally and prediction, then were analyzed computationally. Pathway analysis used Cytoscape software. The functional analysis was evaluated using STRING (Search Tool for the Retrieval of Interacting Genes/Proteins) database. The consequence of increased intracellular Ca2+ content is the increases of oxidative stress, ROS (Reactive Oxygen Species) and the translocation of the protein kinase Cα (PKRCA) to the plasma membrane as the initial step of their activations. This study reveals that activation NRF2 by Ca2+ intracellular which increases in diabetic condition through PRKCA, PRKCA phosphorylate NRF2 in its Neh2 domain at Ser-40. [ABSTRACT FROM AUTHOR]

The high mortality and morbidity rates of malaria remains to be a global problem, particularly in tropical regions. Cerebral malaria, the leading cause of death, possesses a complex pathogenesis involving host immune and parasite interactions. Lately, according to the gut-brain axis concept, the gut microbiota was identified to protect the host against systemic pathogen infection through the modulation of immune signaling pathways. In this study, we aimed to explore the effect of probiotic supplementation toward neurological outcomes of cerebral malaria in vivo. Twenty-eight male C57BL/6 mice were randomly divided into 5 groups after 2 weeks acclimatization period. All groups except the negative control were given oral probiotic supplementation with either Lactobacillus casei at 109 colony forming unit/100 µL (CFU/100 µL), Bifidobacterium longum at 109 CFU/100 µL, or the combination of each at 5x108 CFU/100 µL for five days before to six days after Plasmodium berghei infection. Neurological manifestations were recorded by using the SmithKline, Harwell, Imperial College, Royal Hospital, Phenotype Assessment (SHIRPA) score. The data was analyzed by using One-way ANOVA and post-hoc LSD. The results showed that there were significant differences of motor behavior (P < 0.01), muscle tone (P < 0.01), reflex and sensory (P < 0.05), and neuropsychiatric state (P < 0.05) between the control group and that receiving combination of L. casei and B. longum. The results suggested that probiotic supplementation showed promising effects in improving the neurological manifestations during cerebral malaria. Further studies are required to explicate the pathways involved in the immunological interplay. In the future, it is propitious that the mortalities by cerebral malaria could be mitigated through the optimal use of probiotics. [ABSTRACT FROM AUTHOR]

Background. Pain is one of the most common health problems often experienced by the elderly. Research on pain and its comorbidities in elderly are crucial, because it relates to disability and impairment in quality of life. Unfortunately research related to pain in the elderly and co morbidities are still limited in Indonesia, better understanding about pain can be a vital consideration of treatment and drug consideration. This study was conducted to provide an overview of pain and its comorbidities in elderly. Materials and methods. This was an observational cross sectional study to 949 subjects. Subjects were recruited from outpatients neurology department in 10 big cities Hospitals in Indonesia. Data obtained from subjects who completed the self-made pain questionnaire and medical records. Subjects were characterized based on type of pain, location of pain, properties of pain, time of exacerbation, and comorbidities. All data were processed descriptively. Results and conclusion. A total of 949 subjects met the inclusion criteria. The subjects consist of 476 (50.16%) female and 473 (49.84%) male, with common age group between 60-74 years (80.08%). The Most common pain characteristic are mild pain intensity (46.79%), mixed type of pain (44.68%), chronic pain (64.59%), multiple site pain location (45.42%) and uncertain time pain exacerbation (37.40%). Six hundred and eighteen (65.12%) subjects have comorbidity and 331 (34.88%) without comorbidity. The most common comorbidity found were hypertension (41.31%) and diabetes (14.23%), with 561 (90.78%) subjects have 1 comorbidity. These study provide the overview of the geriatric problems especially in pain and the comorbidities so that the clinician can provide a comprehensive management of pain problems in geriatric. [ABSTRACT FROM AUTHOR]