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The activity of ketoconazole (K, R04 1400 molecular weight 531.44), miconazole (M, R01 4889, molecular weight 479.15) and itraconazole (I, R05 1211, molecular weight 705.64) obtained from Janssen Pharmaceutica NV, against Candida albicans strains was studied. The axenic fungal strains were isolated from the faecal samples of children--not previously treated with antifungal drugs--with clinical symptoms suggesting the inflammation of the mucous membrane in the stomach and/or duodenum. The strains were assessed by own methods and unified tests (bioMérieux: API 20 C, API 20 C AUX). Five codes of Candida albicans Berkhout were found. The minimal inhibitory concentration (MIC) values were calculated from 448 dose-response curves according to the agar diffusion--Kadłubowski's method. MICs were also examined by analyzing the variation (min-max, x mean +/- standard error, mode Mo, median Me). It was demonstrated that all the strains used in the study were susceptible to the azoles drugs.

Analyse of fungi prevalence and their species in the digestive tract of adult persons and children. 1081 faecal sample were examined, 723 from adult persons and 358 from children. Fungal strains were recovered in 67.9% samples from adult persons and in 69.8% from children. There were found 8 species of fungi: Candida albicans, C. famata, C. tropicalis, C. krusei, C. glabrata, C. guilliermondii, C. kefyr, Saccharomyces cerevisiae.

112 strains of Candida albicans were isolated from oral cavity and ontocenoses of the upper digestive tract (endoscopy) of children (age: 5-17) with gastrointestinal disorders. Axenic strains were differentiated with API 20C AUX and API ZYM tests (bioMérieux). Then enzymograms and biotypes were determined for all the strains based on the activity of 19 hydrolases. The highest activity was noted for: e2 - phosphatase alcaline, e6 - leucine arylamidase, e11 - phosphatase acid, e5 - lipase (c14); e7 - valine arylamidase, e12 - naphtol-AS-BI-phosphohydrolase, e16 - alpha-glucosidase and e18 - N-acetyl-beta-glucosamidase, and the latter four were used for biotyping procedures. Our own system was based on the mathematical binominal distribution formula (1 : 4 : 6 : 4 : 1): all "+"; one "-", three "+"; two "-", two "+"; three "-", one "+"; all "-". We have found the following biotypes: A (16.1 +/- 3.5%), B1 (2.7 +/- 2.53%), B3 (8.0 +/- 2.5%), B4 (22.3 +/- 3.9%), C2 (1.8 +/- 1.3%), C3 (7.1 +/- 2.4%), C6 (30.4 +/- 4.3%), D, (11.6 +/- 3.0%).