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The purpose of this paper is to analyze the floristic composition, abundance, biological spectrum and ecological effects of Futoški Park trees and shrubs on the basis of bioindicators. The field research was conducted in Futoški Park, which is one of the oldest and largest parks in the City of Novi Sad, covering an area of 81,306 m2. Upon determining the floristic composition of Futoški Park and the protection zone around the Park hotel, a total of 121 genotypes were recorded, out of which 34 species and lower taxa belong to the Gymnosperm phylum (Pinophyta) and 87 species and lower taxa belong to the Angiosperm phylum (Magnoliophyta). A total of 5,228 representatives of dendroflora were found. The biological range of trees and shrubs in the study area mostly includes deciduous nanophanerophytes (34.98%) and evergreen nanophanerophytes (33.72%), whereas the remainder includes evergreen phanerophytes (16.35%) and the least prevalent deciduous phanerophytes (14.94%). The analysis of ecological indices shows that the greatest number of species meet the environmental requirements, and are successfully acclimated to the climatic and soil conditions. On the basis of the overall vitality and ornamental features of the dendroflora analyzed, it can be argued that Futoški Park is a unique ecological and environmental entity in the urban structure of the city.