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Because of exception properties, inorganic halide perovskites are promising materials for numerous applications. The efficiency of these materials are evaluated based on their photoluminescence quantum yield, which is the key indicator and proportional to the stability of the perovskite. Hence, to limit the instability of the perovskites, addition of surfactant as ligand has been applied during synthesis of nanoparticle based inorganic perovskite CsPbBr 3 . As far as we know, only a few researchers have studied the impact of hexadecylpyridinium bromide (CPB) on the stability of the crystal structure of CsPbBr 3 . In this work, we present a novel approach for lead halide perovskites by incorporating CPB to primarily maintain the perovskite's crystal structure, and later on to enhance the stability of CsPbBr 3 NPs while boosting its photoluminescence quantum yield (PLQY). Results showed that CPB enhanced the thermal stability and boosted the PLQY to 90 %. Moreover, CPB had proven its efficiency as a capping agent preventing the exchange of anion between bromide and iodide ions in presence of lead iodide., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors.)

Background: North Carolina immunizing pharmacists are authorized to provide oral and transdermal hormonal contraception to eligible patients. In March 2022, implementation of this enhanced patient care service began statewide, after approval of standing orders and published training from the North Carolina Association of Pharmacists. Two pharmacy locations on a college campus began offering pharmacist-provided hormonal contraception shortly after approval., Objective: The objective of this study was to assess the willingness of students to seek pharmacist-provided hormonal contraception and the willingness of students to pay for this service., Methods: This cross-sectional study was conducted on the campus of a public, research-intensive university. To be included, individuals must have been 18 years of age or older, enrolled as a student or postdoc at the university, and must have used prescribed hormonal oral contraception within the last year. Individuals who declined consent or surveys that were not at least 90% complete were excluded. The survey was administered via Qualtrics and distributed via e-mail and printed flyers with a QR code. The survey opened February 10, 2023, and closed April 24, 2023., Results: In total, 170 survey responses were analyzed. Almost three-fourths of participants (72.9%) were not aware that pharmacists could provide hormonal contraception in North Carolina. All participants identified at least one benefit to pharmacist-provided contraception, with the most common responses being "I believe it would save time" (85.3%) and "appointment not needed" (89.4%). Almost two-thirds of participants (65.3%) identified at least one barrier that would prevent them from seeking pharmacist-provided contraception. The most common barrier identified was privacy at the pharmacy (20.0%). Most participants (81.8%) reported that they are willing to pay for this service, with the majority willing to pay up to $29., Conclusion: Students on this campus seem to have a receptive attitude toward pharmacist-provided hormonal contraception and are willing to pay for a consultation., Competing Interests: Disclosure Macary Marciniak declares that her spouse is employed by the American Pharmacists Association (APhA). The authors declare no other relevant conflicts of interest or financial relationships., (Copyright © 2024 American Pharmacists Association®. Published by Elsevier Inc. All rights reserved.)

Objectives: Tobacco-free campus policies are needed to create environments conducive to prevention or quitting in the Eastern Mediterranean Region (EMR), where the use of various tobacco products is on the rise among university students. The objectives of this study were to assess overall support for a 100% tobacco-free campus policy and its predictors among different stakeholder groups at the largest national university in Qatar and to understand attitudes towards specific policy components., Study Design: A cross-sectional online survey of faculty and students., Methods: We measured sociodemographic variables, tobacco use, and support for components of a 100% tobacco-free campus policy. A total score for support was calculated, and associations with selected predictors were assessed using multivariable linear regression., Results: 413 respondents participated in the survey. A majority of faculty/staff and students (76.6% and 75.5%, respectively) supported the implementation of a 100% tobacco-free campus policy, with a majority supporting extension of the policy beyond cigarettes. Support for other components of the policy varied. Among students, overall support was lower among males and tobacco users and higher among the married. Among faculty and staff, support was lower among Qatari nationals and tobacco users., Conclusion: In the context of a growing tobacco crisis in the EMR, implementing and understanding the impact of tobacco-free campus policies is essential. This paper underscores the importance of addressing diverse stakeholder attitudes and providing empirical evidence to guide policy implementation and evaluation., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors.)

Inorganic halide perovskites, such as CsPbI 3 , have unique optoelectronic properties which made them promising candidates for several applications. Unfortunately, these perovskites undergo rapid chemical decomposition and transformation into yellow δ-phase. Thus, the synthesis of stable cesium lead iodide perovskites remains an actual challenging field and it is imperative to develop a stabilized black phase for photovoltaic applications. For this purpose, a surfactant ligand was used to control the synthesis of inorganic perovskite CsPbI 3 nanoparticles. Herein we demonstrate a new avenue for lead halide perovskites with the addition of either hexadecyltrimethylammonium bromide (CTAB) or silica nanoparticles to maintain in the first place; the stability of the α-CsPbI 3 phase, and later on to boost their photoluminescence quantum yield (PLQY). The prepared perovskites were characterized using UV-visible absorption spectroscopy, fluorescence spectroscopy, scanning electron microscopy, thermogravimetric analysis and X-Ray diffraction technique. Results show higher stability of α-CsPbI 3 phase and improvement in PLQY % to reach 99% enhancement in presence of CTAB. Moreover, the photoluminescence intensity of CsPbI 3 nanoparticles was higher and was maintained for a longer duration in the presence of CTAB., (© 2023. The Author(s), under exclusive licence to European Photochemistry Association, European Society for Photobiology.)

Background: This survey aimed to investigate routine practices and approaches of clinicians on pediatric airway in anesthesia and intensive care medicine., Methods: A 20-question multiple-choice questionnaire with the possibility to provide open text answers was developed and sent. The survey was sent to the members of European Airway Management Society via a web-based platform. Responses were analyzed thematically. Only the answers from one representative of the pediatric service of each hospital was included into the analysis., Results: Among the members, 143 physicians responded the survey, being anesthesiologists (83.2%), intensivists (11.9%), emergency medicine physicians (2.1%), and (2.8%) pain medicine practitioners. A straight blade was preferred by 115 participants (80.4%) in newborns, whereas in infants 86 (60.1%) indicated a curved blade and 55 (38.5%) a straight blade. Uncuffed tracheal tube were preferred by 115 participants (80.4%) in newborns, whereas 24 (16.8%) used cuffed tubes. Approximately 2/3 of the participants (89, 62.2%) reported not to use routinely a cuff manometer in their clinical practice, whereas 54 participants (37.8%) use it routinely in pediatric patients. Direct laryngoscopy for routine pediatric tracheal intubation was reported by 127 participants (88.8%), while 16 (11.2%) reported using videolaryngoscopes routinely. Interestingly, 39 (27.3%) had never performed neither videolaryngoscopy nor flexible bronchoscopy in children. These results were significantly less in hospitals with a dedicated pediatric anesthesiologist., Conclusions: This survey on airway management in pediatric anesthesia revealed that the use of cuffed tubes and the routine monitoring of cuff pressure are rare. In addition, the rate of videolaryngoscopy or flexible optical intubation was low for expected difficult intubation. Our survey highlights the need for properly trained pediatric anesthesiologists working in-line with updated scientific evidence.

Sciatica is a chronic condition causing crippling low back pain radiating down to the sciatic nerve innervation area, which is the posterior thigh. It remains a major public health problem worldwide with significant socio-economic, physical, and psychological impacts. Studies suggested different diagnostic methods due to the lack of consensus on diagnostic and treatment guidelines. When it comes to the management and treatment, there is ambiguous evidence about the use of painkillers, surgical interventions, and alternative options and their effectiveness, with most studies contrasting one another in addition to the lack of high-quality trials. This review presents the available data on the current understanding of sciatica covering clinical manifestations, diagnosis and treatment modalities, prognosis, and complications since a disagreement is observed in the scientific community regarding sciatica, starting with a definition of sciatica, its epidemiological characteristics, to the management and treatment. Our review would help raise knowledge and awareness about sciatica in the health professional community and the general public since the prevalence of low back pain is high in most parts of the world and there is insufficient knowledge of sciatica in the literature., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2022, Fairag et al.)

Poly Lactic-Co-Glycolic Acid (PLGA) and Poly Diallyldimethylammonium Chloride (PDDA) are widely being used for drug delivery and curcumin is being studied as potential drug molecule for its anti-oxidant, anti-inflammatory and anti-cancer activities. The interaction between PLGA, PDDA and curcumin was investigated by fluorescence spectroscopy. The modified Stern-Volmer equation was used to estimate the value of the binding constant Ka and the van't Hoff equation was used to estimate the corresponding thermodynamic parameters (ΔH o , ΔS o , and ΔG o ). The obtained results showed that the binding constant between PLGA and Curcumin is due to the formation of hydrogen bonds and van der Waals forces. However, PDDA interacts with curcumin through hydrophobic interactions. Moreover, zeta potential measurements were obtained for these polymers and the surface charge was compared in presence and absence of the negatively charged curcumin molecules. It was found that the results obtained by zeta potential measurements are in agreement with those obtained by fluorescence spectroscopy. It is also found that binding of curcumin with PDDA is further encouraged in the presence of PLGA., (© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

The main purpose of this paper is to design curcumin loaded PLGA nanocapsules for the selective detection of dopamine using fluorescence spectroscopy. In the present work curcumin loaded PLGA nanocapsules were synthesized using a solid-in-oil-in water (s/o/w) emulsion technique. The prepared nanocapsules were coated with a poly(diallyldimethylammonium)chloride (PDDA) polymer to increase the entrapment of curcumin into the core of PLGA polymer. PLGA-Cur-PDDA nanocapsules were characterized using different microscopic and spectroscopic techniques. Unlike free curcumin, the formed CUR-PLGA-PDDA NCs were established as nanoprobes for the selective detection of dopamine molecules. The selectivity and specificity of nanocapsules toward dopamine was achieved by measuring the fluorescence emission spectra of the NCs in the presence of other interference molecules such as tryptophan, melamine, adenine, etc. It was noticed that increasing the concentration of the different molecules had no significant change in the fluorescence signal of the nanocapsules. These results confirm the strong quenching between dopamine and curcumin in the nanocapsules. Hence, this fluorescence emission technique was found to be selective, easy and fast with low cost for the determination of dopamine in a concentration range up to 5 mM with a detection limit equal to 22 nM., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)

Nanosystems with various compositions and biological properties are being extensively investigated for drug and gene delivery applications. Many nanotechnology methods use novel nanocarriers, such as liposomes, in therapeutically targeted drug delivery systems. However, liposome matrices suffer from several limitations, including drug leakage and instability. Therefore, the surface modification of liposomes by coating them or adding polymers has advanced their application in drug delivery. Hence, the prevention of drug release from the liposome bilayers was the main focus of this work. For this purpose, liposomes were synthesized according to a thin film hydration method by applying various surface modifications. Three different nanocapsules, N1, N2, and N3, were prepared using 1,2-dimyristoyl- sn-glycero -3-phosphocholine (DMPC), poly(diallyldimethylammonium)chloride (PDAA) polymer, and silica nanoparticles. PDDA and silica nanoparticles were coated on the surface of liposomes using a layer-by-layer assembly method, completely encapsulating curcumin into the core of the liposome. Fluorescence spectroscopy, TGA, DLS, XRD, SEM, and zeta potential methods were used to characterize the prepared nanocapsules. Interestingly, the fluorescence of curcumin showed a blue shift and the fluorescence efficiency was extraordinarily enhanced ∼25-, ∼54-, and ∼62-fold in the N1, N2, and N3 nanocapsules, respectively. Similarly, encapsulation efficiency, drug loading, and the anticancer activity of dietary curcumin were investigated for the different types of DMPC nanocapsules. The drug efficiencies of the liposomes were established according to the release of curcumin from the liposomes. The results showed that the release of curcumin from the nanocapsules decreased as the number of layers at the surface of the liposomes increased. The release of curcumin follows the Higuchi model; thus, a slow rate of diffusion is observed when a number of layers is added. The better encapsulation and higher anti-cancer activity of curcumin were also observed when more layers were added, which is due to electrostatic interactions inhibiting curcumin from being released., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)

Understanding the self-assembly behavior of block copolymers is of great importance due to their usefulness in a wide range of applications. In this work, the physical properties of poly(lactic- co -glycolic acid) (PLGA polymer) are studied for the first time in solution using the fluorescence technique and curcumin as a molecular probe. First, curcumin at a concentration of 2 μM was added to different concentrations of PLGA, and the fluorescence of curcumin was tracked. It was found that the critical micellar concentration (CMC) was equal to 0.31 g/L and the critical micellar temperature (CMT) was obtained to be 25 °C. Furthermore, an insight on the effect of NaCl salt on the CMC value of PLGA is assessed through curcumin probing. A decrease in the CMC has been observed with the increase in the concentration of NaCl, which could be due to the salting out effect. Moreover, in order to understand the aggregation behavior of PLGA in different solutions, CMC experiments were investigated using chloroform as a solvent. Results showed that the solvent does not affect the CMC value of the polymer; however, it only affects the shape of the obtained micelle forming a reversed micelle. Finally, fluorescence quenching of curcumin with hydrophobic cetyl-pyridinium bromide (CPB) and hydrophilic KI quenchers was established, where it was proved that curcumin is located near the hydrophobic pocket of the Stern layer of the PLGA micelle., Competing Interests: The authors declare no competing financial interest., (© 2022 The Authors. Published by American Chemical Society.)

One of the limitations of fluorescence probe molecules during biomedical estimation is their lack of ability to selectively determine the targeted species. To overcome this there have been various approaches that involve attaching a functional group or aptamers to the fluorescence probe. However, encapsulating probe molecules in a matrix using nanotechnology can be a viable and easier method. Curcumin (Cur) as a fluorescence marker cannot distinguish DNA and RNA. This research reports a novel selective approach involving the use of nanocapsules composed of liposomal curcumin coated with chitosan for the selective detection of RNA molecules using a fluorescence method. The increase in RNA concentration enhanced the electrostatic interaction between the negatively charge surface of RNA and the positively charged nanocapsule, which was further verified by zeta potential measurement. This method had a low limit of detection (36 ng/ml) and higher linear dynamic ranges compared with other studies found in the literature. Moreover, the method was not affected by DNA and was selective for the detection of RNA molecules for which the site of interaction was confined only to uracil. The selectivity for RNA molecules towards other analogues species was also examined and recovery range found was between 99 and 100.33%., (© 2022 John Wiley & Sons Ltd.)

Background: Recent data show an increase in the prevalence of waterpipe tobacco smoking (WTS) among university students in the Arab Gulf region alongside an attitude of social acceptability and a perception that WTS is less harmful than traditional cigarette smoking. Objective: This study measures the prevalence of WTS among university students and examines the individual, sociocultural, and environmental factors influencing this practice. Methods: Participants were selected through stratified random sampling of students in the largest national university in Qatar. Data were collected anonymously using an online questionnaire. Descriptive univariate and bivariate analyses were conducted to examine the association of WTS with participants' sociodemographic characteristics, knowledge, and attitudes. Results: 199 students completed the questionnaire. Among students who reported using tobacco products, waterpipe ( shisha ) was the most common product (70.6%). WTS was significantly associated with having a mother ( p  = 0.015) or a close friend ( p < 0.001) who smoked. Compared to non-users, waterpipe tobacco users were significantly more likely to believe that waterpipe tobacco is less addictive than cigarettes (p = 0.009) and significantly less likely to believe that waterpipe tobacco can lead to cardiovascular diseases (p = 0.003) or dental problems (p = 0.02). More waterpipe tobacco users than non-users disagreed that parents (p = 0.005) or advertisements (p = 0.002) could influence use. More waterpipe tobacco users (70%) than non-users (37%) believed that females were more comfortable using waterpipes than cigarettes. Conclusions and implications: The findings shed light on factors shaping WTS and provide evidence for designing multilevel behavioral interventions to decrease the prevalence of WTS among youth.

Background: Electronic cigarette (e-cigarette) use is becoming more popular worldwide, especially among youth. Studies report that university students have inadequate knowledge as well as misconceptions about the health risks of e-cigarettes, which may lead to their use even in populations where smoking prevalence is relatively low. At this age, the influence of peers is also significant. Understanding attitudes of university students toward the use of e-cigarettes is important for effective tobacco prevention interventions. In this study, we assess the prevalence of e-cigarette use among students in Qatar's largest national university, as well as their knowledge, attitudes, and perceptions of harm in relation to e-cigarettes. Methodology: We conducted a cross-sectional study among Qatar University students using a self-administered online questionnaire. Descriptive univariate analyses were conducted as well as bivariate analyses to check the association of e-cigarette use with variables of interest. A binary logistic regression analysis was conducted to assess determinants of e-cigarette use among students. Results: One hundred ninety-nine students completed the questionnaire. The prevalence of e-cigarette use among students was 14%, with no significant difference by gender (16.2% in males and 12.8% in females). In bivariate analyses, significantly fewer e-cigarette users believed that e-cigarettes cause disease compared to non-users. 67.9% of e-cigarette users compared to 37.6% of non-users believed that e-cigarettes were less harmful than traditional cigarettes, and 78.6% of users compared to 40.4% of non-users believed that their use could be helpful in preventing smoking traditional cigarettes. Bivariate associations between e-cigarette use and knowledge items were significant ( p < 0.05) as well as having a smoker among siblings or friends. In the multivariate analysis, only having a friend who was a smoker remained significant after controlling for other variables (OR = 7.3, p < 0.001). Conclusion: Our study found that university students have knowledge gaps and misconceptions with regard to the harms associated with e-cigarettes use, especially among users. A comprehensive smoking prevention policy, educational interventions, and quit support are needed to enhance awareness among university students about the health effects associated with e-cigarettes use. Such interventions should also take into account the influence of peers on smoking practices., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Kurdi, Al-Jayyousi, Yaseen, Ali, Mosleh and Abdul Rahim.)

Congo red is one of the common organic dyes that is found in water as waste of the industrial work. The use of congo red has long been of great concern, primarily because of its carcinogenic properties. Congo red can be isolated and removed from water by adsorption using nanoparticles. The use of zinc curcumin oxide, also known as curcumin conjugated zinc oxide, nanoparticles was elaborated for the first time in this work for this purpose. The optimization of the synthesis reaction of zinc curcumin oxide nanoparticles was established by modifying the flow rate of KOH, pH of the medium, different temperature, and in the presence or absence of chitosan polymer. These nanoparticles were characterized through SEM, UV-Visible absorption Spectroscopy, fluorescence spectroscopy, TGA, and XRD. It is found that during synthesis, addition of KOH dropwise in alkaline media improved the stability of the formed nanoparticles. Similarly, addition of chitosan has further increased their stability with only 10% mass loss. The importance of the formed nanoparticles was investigated by analyzing their efficiency in the adsorption of congo red where Zn(Cur)O had an adsorption capacity equal to 89.85 mg/g, which is one of the highest reported in literature, following the pseudo second order model. Nevertheless, negative surface charge of congo red and positive surface charge of Zn(Cur)O may also get supported by π-π interaction between curcumin and congo red that encourages adsorption in zinc curcumin oxide which is obstructed in the presence of chitosan., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Background: Published evidence is lacking to describe the pharmacist's role in medication management within an attention deficit hyperactivity disorder (ADHD) specialty clinic., Objectives: The objectives were (1) to measure the growth of an ADHD clinic in a college health center after the integration of clinical pharmacists and (2) to evaluate provider adherence to clinic policies and procedures before and after pharmacist integration., Practice Description: In 2017, a pharmacist-run ADHD clinic was established at a college campus., Practice Innovation: Pharmacists conducted collaborative initial visits with psychiatrists and independently provided follow-up appointments for patients with ADHD., Evaluation Methods: Data were extracted from the electronic health record for patients aged 18 years or older with an ADHD diagnosis who completed a medication evaluation or medication follow-up visit from July 1, 2016 to June 30, 2019. Data were excluded if it was for another visit type, was a non-ADHD clinic provider visit, or if the visit note was classified. Data before the pharmacist integration (July 1, 2016-June 30, 2017) were compared with data after the pharmacist integration (July 1, 2017-June 30, 2019). Chi-square tests of independence evaluated differences in blood pressure monitoring, heart rate monitoring, and stimulant medication contract signature between psychiatrist- and pharmacist-run appointments., Results: Pharmacist presence in the ADHD clinic increased from 0 full-time equivalent (FTE) to 0.2 FTE over 3 years. The number of appointments increased by 1003% (from 26 to 287). Compared with psychiatrist-run appointments, pharmacist-run appointments were more adherent to monitoring blood pressure (11% vs. 77%, P < 0.001) and heart rate (6% vs. 75%, P < 0.001), as well as clinic policy requiring a patient's signature on a stimulant medication contract (64% vs. 75%, P = 0.019)., Conclusion: Pharmacists can assist psychiatrists in medication management of ADHD in the college health setting. A pharmacist-psychiatrist collaboration increased quality of care and monitoring of medication adverse effects., (Copyright © 2021 American Pharmacists Association®. Published by Elsevier Inc. All rights reserved.)

Objectives: Effective policies are an important tool for reducing tobacco use. We examine student perceptions of the existing no-smoking policy in the country's largest national university and identify perceived barriers to its implementation. We explore student support for a 100% tobacco-free campus policy., Design: We conducted a cross-sectional survey using a self-administered on-line questionnaire., Setting: This study was conducted at Qatar University (QU), the largest national institution of higher education in Qatar., Participants: A total of 199 students participated out of a stratified random sample of students aged ≥18 years with active registration in the Spring 2020 semester., Primary and Secondary Outcome Measures: We assessed students' knowledge of and attitudes towards QU's current no-smoking policy, the perceived barriers to its implementation, and support for a 100% tobacco-free policy., Results: Only 26% (95% CI 19.97 to 32.03) and 16.6% (95% CI 11.70 to 22.49) of respondents correctly identified the current policy on traditional and electronic cigarettes, respectively. Less than 30% of respondents held positive attitudes towards policy enforcement, and more male respondents reported positive attitudes towards compliance than women. Support for a 100% tobacco-free policy was at 77.2%, but it was significantly lower among tobacco users compared with non-users (35.9% and 91.8%, respectively; p<0.001). Failure to establish clear penalties, opposition from smoking students, and lack of cessation services were perceived as major barriers to implementation., Conclusions: Clear and comprehensive tobacco-free policies are important tools for creating environments conducive to rejecting smoking and seeking cessation support. The findings underscore the need to increase awareness about the policy, advocate for clear penalties for violations, and promote cessation services on campus. Qualitative research is needed to further understand perceived barriers to successful enforcement of the policy., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)

Despite efforts to utilise bauxite residue, the amount of red mud stored in reservoirs is increasing. This paper aims to evaluate the potential of red mud and other sludge waste types as a soil substitute by monitoring plant development. Pot experiments were carried out testing two types of mixtures: dredging sludge from Lake Balaton mixed with garden soil and the sewage sludge and soil blend. These were then treated with red mud (15 and 30% w/w). The plants were under-, while the roots were more developed in the sewage sludge mix than the dredging sludge blend and the control soil. In the sewage amendment, the phosphorous content increased while the calcium content was lower than in the other soil types and the optimum. The metals uptake of the plants was a factor of the red mud quantity. Lead, nickel, titanium and silicon had elevated concentrations parallel to higher red mud content, but only the nickel exceeded the threshold of the Hungarian legislation. Silicon and titanium were beneficial for plant growth, compensating for the potentially toxic effects of lead and nickel. Results suggest that the red mud in a mixture with either sewage sludge or dredging sludge can act as catalysts for the growth rate of test plants, allowing their utilisation as secondary raw materials., (Copyright © 2021 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

The developing public interest in traditional medicine, especially plants-based drug, has prompted extensive research on the potential of naturally existing compounds. Among these compounds, curcumin is currently one of the most studied substances. In this study, we elaborate the physical properties of diarachidonyl phosphatidyl choline (DAPC) liposome using fluorescence method, where curcumin at low concentration was used as a probe molecule. In the first place, the phase transition temperature of DAPC was determined by following the fluorescence intensity of curcumin as a function of temperature, along with evaluating the effect of concentration of curcumin in the presence or absence of chitosan oligosaccharide lactate as an additional protective layer. On the other hand, quenching reactions using CPB and KI as quenchers reflected the ease of entry of different concentrations of these quenchers to the curcumin located in the hydrophobic core of the liposome which give new insight about the lipophilicity and permeability of the DAPC membrane. Finally, the partition coefficient analysis was investigated. It was concluded that curcumin has a higher partition coefficient at a temperature above the phase transition temperature of DAPC liposomes where the liposome is in the fluid liquid crystalline phase. Modulation of liposomes properties in the presence of chitosan oligosaccharide lactate layer was for the first time investigated. Chitosan oligosaccharide lactate acts as protecting layer without changing the phase transition temperature, but it affects the membrane permeability depending on solid gel and liquid crystalline phase., (Copyright © 2020 Elsevier B.V. All rights reserved.)

In the present work, a novel analytical procedure by integrating principal coordinate analysis (PcoA) with excitation-emission matrix fluorescence (EEMF) spectroscopy was introduced for discriminating the commercial gasoline fuels. The PcoA technique involved analysis of the distance matrices containing the dissimilarity information and it can serve as an efficient tool for capturing the major as well as subtle compositional differences among the analyzed commercial gasoline samples. The utility of the proposed PcoA assisted EEMF analytical procedure was successfully tested by discriminating gasoline fuel samples belonging to five different industrial brands. The obtained results clearly showed that combination of PcoA and EEMF could provide a simple, sensitive and economical analytical procedure to carry out the rapid analyses of the gasoline samples belonging to different brands.

Ascorbic acid was used to reduce cystine to cysteine that induces the aggregation of glutathione-capped copper oxide nanoparticles. The aggregation of CuO NPs was optimized through resonance Rayleigh scattering and dynamic light scattering measurements. The high specificity toward cysteine from other amino acids and biomolecules was due to its mercapto group that binds to the surface of CuO NPs and the electrostatic interaction between the cysteine zwitterions on the surface of CuO NPs. Accordingly, glutathione-capped copper oxide nanoparticles was used as a sensing probe for cystine based on resonance Rayleigh scattering (RRS) technique. Increase in the RRS signal of CuO NPs was observed with increasing cystine concentration. A linear calibration plot was obtained in the range 2-20 μM with a limit of detection of 4.55 ± 0.5 nM, which is lower than literature value. The applicability of the proposed sensing strategy toward cystine was established, and the recovery percentage was between 99.8 ± 0.4 and 101.0 ± 2.1 for n = 3. Graphical Abstract .

In a novel approach, curcumin has been encapsulated inside Poly(Ethylene Oxide)-Block-Poly(Propylene Oxide)-Block-Poly(Ethylene Oxide) (F108) nanocapsules. FTIR spectra have indicated a type of hydrogen bonding and dipole interaction between curcumin and F108. Fluorescence and UV-visible absorption profiles of curcumin in nanocapsules have indicated location of curcumin in more hydrophobic microenvironment. The relative fluorescence yield has increased by 6 times in the nanocapsules, which renders them as more sensitive probes to be used later on in sensing study. Therefore, based on the functionality of curcumin as a fluorescent transducer, encapsulated curcumin is used in biomedical application as DNA and RNA sensing. Detection limits are detected as 50 μM and 60 μM for DNA and RNA respectively. Linear dynamic concentration range obtained in this proposed method is much higher than reported in literature. The interaction between the nanocapsules and targeted DNA/RNA molecules is further approved by zeta potential studies. Furthermore, the real interaction of DNA with the encapsulated curcumin is confirmed by the interaction of the adenine and cytosine nucleotides. This has been verified through zeta potential measurements. Moreover, our prepared nanocapsules has presented a high percentage recovery of DNA and RNA (96-101%). Finally, stability results have illustrated a high photostability of encapsulated curcumin, indicating that proposed nanocapsules can be considered as a stable sensor during measurement time.

Background: Antimicrobial resistance (AMR) is a serious public health problem and a global concern. The inappropriate use of antibiotics has been identified by the World Health Organization as a major risk factor for AMR., Methods: The purpose of this research study is to assess the prevalence of inappropriate antibiotic use among Qatar University students and their family members, detect sociodemographic factors associated with inappropriate use, evaluate the knowledge and attitude towards antibiotic use, and assess respondents' opinions on healthcare providers' antibiotic prescription practices. Participants (N = 596) completed a self-administered questionnaire. Descriptive analysis, the Pearson chi-squared test, and multivariate logistic regression analyses were performed., Results: The major inappropriate antibiotic use practices followed by the respondents were using antibiotics without prescription (82%), not completing the antibiotic course (45%), and obtaining antibiotics from the pharmacy without prescription (23%). The chi-square test results showed that age (p = 0.031) and nationality (p = 0.041) were associated with using antibiotics without prescription. In addition, respondents less than 21 years of age (p<0.001), who had only a secondary education (p = 0.007), and who lived in one of the large and crowded cities in Qatar (p = 0.011) had higher odds of stopping the antibiotic before completing the course. Our study also revealed that almost 60% of the respondents had inadequate knowledge and a negative attitude towards antibiotic use. Nationality and municipality were the independent factors associated with having appropriate knowledge of antibiotic use. Univariate logistic regression analyses in our study demonstrated that older (>26 years), married and university-graduated participants were more likely to have a positive attitude towards antibiotic use than others. Respondents also reported that neither doctors nor pharmacists were providing adequate patient education about appropriate antibiotic use. The Socio-Ecological Model was applied to interpret the findings and frame implications., Conclusion: The findings shed light on various factors shaping antibiotic use practices and provide evidence to design multilevel behavioral interventions to improve public practices of antibiotic use., Competing Interests: The authors have declared that no competing interests exist.

This review (with 116 refs.) summarizes the state of the art in resonance Rayleigh scattering (RRS)-based analytical methods. Following an introduction into the fundamentals of RRS and on the preparation of metal nanoparticles, a first large section covers RRS detection methods based on the use of gold nanoparticles, with subsections on proteins (albumin, bovine serum albumin and ovalbumin, glycoproteins, folate receptors, iron binding-proteins, G-proteins-coupled receptors, transmembrane proteins, epidermal growth factor receptors), on pesticides, saccharides, vitamins, heavy metal ions (such as mercury, silver, chromium), and on cationic dyes. This is followed by a section on RRS methods based on the use of silver nanoparticles, with subsections on the detection of nucleic acids and insecticides. Several Tables are presented where an RRS method is compared to the performance of other methods. A concluding section summarizes the current status, addresses current challenges, and gives an outlook on potential future trends. Graphical Abstract Change in the resonance Rayleigh scattering (RRS) intensity when mixing the nanoparticles with the specific analyte.

Objectives: The primary objectives of this study were to determine the percentage of travel recommendations accepted by individuals serviced at a university travel health clinic and to identify barriers to travel recommendation acceptance or implementation by travelers. A secondary objective was to use details regarding the identified barriers to refine travel clinic protocols., Methods: This cross-sectional study utilized an 11-item questionnaire, conducted via telephone from February 2018 to April 2018. The survey was administered by a pharmacist employed at the travel clinic. The following data were collected: travel itinerary, content of pharmacist provided travel recommendation(s), acceptance of pharmacist travel recommendation(s) or reason for declining the recommendation(s), patient utilization of supplemental recommendations (e.g., traveler's diarrhea treatment flowchart, over-the-counter travel items, accessing medical care abroad), perceived usefulness of travel clinic resources, and the status of patient health during travel and upon return. Data was evaluated using descriptive statistics., Results: A total of 205 travelers were screened to participate in the study; 85 surveys were completed, resulting in a 41% response rate. Of 349 travel vaccination recommendations made, 242 (69%) were accepted by patients. The vaccine recommendations with the lowest acceptance were Japanese Encephalitis (18%, n = 2/11), rabies (27%, n = 15/55), and influenza (51%, n = 41/80). The top reasons for declining included cost (40%, n = 34), lack of perceived necessity (37%, n = 31), and timing (16%, n = 14). In addition, 68% reported using travel clinic advice if they became ill during travel., Conclusion: This study showed a positive response to recommendations provided by this travel clinic. Cost and 'lack of perceived necessity' were identified as barriers to acceptance of recommendations. Travel clinic protocols will be refined to provide further education to travelers about unfamiliar disease states that may pose an increased risk during travel, aiming to increase acceptance of pharmacist-provided recommendations., (Copyright © 2019 American Pharmacists Association®. Published by Elsevier Inc. All rights reserved.)

Aims: To assess the knowledge and practices toward diabetes in the Jordanian community., Methods: This study was conducted as a public based cross-sectional study in different cities in Jordan. A previously published validated questionnaire about knowledge, attitudes, and practices (KAP) toward diabetes mellitus (DM) was translated from the Arabic version and used in this study with very minor modification to be suitable for this study of the Jordanian population., Results: A total of 1,702 participants were recruited in the present study. About half of the participants (53.3%) had good knowledge scores. The respondents' knowledge scores were significantly correlated with attitudes (p < 0.001). The education level (university or higher) and education related to a field were predictors for good knowledge and positive attitudes. About 46.3% of participants had positive attitudes toward the disease. As for practices, 37.7% of participants did not engage in regular exercise while more than half of the study subjects had never checked their blood glucose level on an annual basis. The factors influencing the practice of checking blood glucose level have been investigated., Conclusion: This study has highlighted the need for more educational interventions to address negative attitudes and promote healthy lifestyle practices and regular health checks especially in certain subgroups of patients, such as those not having a degree related to the medical field and not having a first-degree relative with DM., Competing Interests: The authors have declared that no competing interests exist.

Polycyclic aromatic hydrocarbons (PAHs) are carcinogenic and mutagenic in nature therefore their sensing in water sample is an important analytical task. In the present work, a novel approach that is based on the random initialisation of the excitation-emission matrix fluorescence (EEMF) spectral variables in constraint fashion for subsequent multivariate curve resolution alternating least Square (MCR-ALS) analysis is introduced for simultaneously sensing the complex dilute aqueous mixture of PAHs. The usefulness of the proposed analytical approach is successfully demonstrated by applying it intentionally on a calibration set that is peculiar in many senses. The peculiarity mainly arises because the designed (i) the calibration set consist of nine PAHS having significant spectral overlap, (ii) the concentration of each PAH in different samples are kept constant and (iii) any two samples differ only in the presence and absence of the PAHs. The proposed approach is found to make precise and accurate estimation of each of the nine PAHs without involving any pre-separation. In summary, the proposed approach provides a simple and cost-effective procedure for simultaneous sensing of several PAHs in water samples. The proposed approach could be very useful in developing countries., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Background: Anaphylaxis is a serious allergic disease that may lead to death if not immediately recognized and treated. Triggers of anaphylaxis including food, drugs, and insect stings can vary widely. The incidence of anaphylaxis seems to be affected by age, sex, atopy, and geographic location. This study aims to examine the common triggers of anaphylaxis in Qatar., Methods: A total of 1068 electronic medical records were audited using power chart system: 446 from the medical coding system of anaphylaxis and 622 from the epinephrine auto-injectors (EAIs) dispensed during January 2012-December 2017., Results: Of 1068 patients, 574 (53.5%) had anaphylaxis; male to female ratio was 1.2, and 300 patients (77.9%) were less than 10 years old. The common triggers were food ( n  = 316, 55.0%), insect stings ( n  = 161, 28.0%), and drugs ( n  = 103, 17.9%). Common anaphylaxis food triggers were nuts ( n  = 173, 30.1%), eggs ( n  = 89, 15.5%), and seafood ( n  = 72, 12.5%), and common anaphylaxis medication triggers were antibiotics ( n  = 49, 8.5%) and nonsteroidal anti-inflammatory drugs ( n  = 30, 5.2%). Interestingly, 135 anaphylactic patients (23.5%) were due to black ant stings. The anaphylaxis triggers varied significantly between children and adults. Among children (less than 10 years), three quarters of the events were triggered by food (223, 74.3%) while among adults (20-55 years), insect stings ( n  = 59, 43.0%) and drugs ( n  = 44, 32.0%) were dominant., Discussion: This is the first national study stratifying anaphylaxis triggers among different age groups in Qatar. This study will serve as a guide for clinical practice in allergy clinics in Qatar and will help to assess future trends of anaphylaxis in Qatar., Competing Interests: Ethical Approval: This retrospective study was ethically approved by the IRB committee of Medical Research Centre- Hamad Medical Corporation (IRB-HMC) (IRB#17122/17) with expedited status. For more information, kindly contact irb@hamad.qa, Tel: 00974–44,390,614.The study was a retrospective study. IRB-HMC approved the study for waiver of consent; provided that the methods used to protect patient confidentiality were judged to be adequate.Not applicable.The authors declare that they have no competing interests.Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

The number of babies in Qatar being exclusively breastfed is significantly lower than the global target set by the World Health Organization. The purpose of this study was to assess knowledge, attitude, and practice (KAP), selected barriers, and professional support as well as their association with continued breastfeeding at one year of age. A sample of Qatari and non-Qatari mothers ( N = 195) who attended a well-baby clinic held at primary health care centers in Qatar completed a self-administered questionnaire. Descriptive analysis, the Pearson Chi-squared test, and logistic regression were performed. Around 42% of the mothers stopped breastfeeding when their child was aged between 0 and 11 months old. Mothers who had only one or female child stopped breastfeeding between the ages of 0 and 6 months ( p = 0.025, 0.059). The more optimal the breastfeeding practices followed by the mothers, the older the age of the infant when they stopped breastfeeding ( p = 0.001). The following factors were inversely associated with breastfeeding duration: the mother's perceptions that she "did not know how to breastfeed," or "wasn't making enough milk," and the need "to return to work/school", with p = 0.022, 0.004, and 0.022, respectively. These findings present factors that should be considered when planning for health education and promotion programs to prolong breastfeeding duration in Qatar.

The authors describe a method for functionalization of gold nanoparticles (AuNPs) with the supramolecular host molecule, curcubit[7]uril (CB[7]) which can bind rhodamine B (RhB). The fluorescence of RhB is quenched by the AuNPs via surface energy transfer. On addition of ATP, a dimeric RhB-ATP complex is formed and RhB is pushed out of CB[7]. Hence, fluorescence increases by a factor of 8. This fluorescence recovery effect has been utilized to develop a new detection scheme for ATP. The assay, measured at fluorescence excitation and emission wavelengths of 500 nm and 574 nm respectively, works in the 0.5-10 μM concentration range and has a 100 nM detection limit. The method is not interfered by UTP, GTP, CTP, TTP, ascorbic acid and glutathione. Graphical abstract Schematic of a method for determination of ATP in the 500 nM to 10 μM concentration range by using fluorescence recovery after surface energy transfer (SET) between rhodamine B (RhB) and gold nanoparticles capped with curcubit[7]uril (CB[7]).

Long-armed multifunctional organosilicon molecules display self-replicating and self-perfecting behavior in asymmetric autocatalysis (Soai reaction). Two representatives of this class were studied by statistical methods aiming at determination of probabilities of natural abundance chiral isotopomers. The results, reported here, show an astonishing richness of possibilities of the formation of chiral isotopically substituted derivatives. This feature could serve as a model for the evolution of biological chirality in prebiotic and early biotic stereochemistry., (© 2018 Wiley Periodicals, Inc.)

Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol) (F-108) functionalized gold nanoparticles (Au NPs) have been successfully synthesized. During synthesis it is found that an increase in the F-108 concentration contributes to agglomeration in the media, increasing the size of the Au particles, and boosting the curcumin concentration leads to a higher density of functional groups, resulting in smaller Au NPs. FT-IR analysis reveals that the hydroxyl and phenolic groups of curcumin and F-108 are involved during the functionalization of Au surfaces. Enhancement in the fluorescence/RRS intensity is due to the combination of the influence of the shape/size of the Au NPs as well as the extent of curcumin conjugation at the interface of the Au NP surface and F-108. The presence of sugar molecules remarkably boosts the RRS intensity without significantly affecting the fluorescence and surface plasmon absorbance of the Au NPs; in contrast, the RRS intensity of standard CTAB functionalized Au NPs is unaffected by glucose molecules indicating that the functionalization of F-108 at Au surfaces is crucial. Interestingly, no interference from other potential interferents and antioxidant substances like ascorbic acid, creatinine and acetaminophen is observed. This method is simple and fast, and offers a wider linear dynamic range, 0-10 mM, that is applicable under physiological conditions and in serum samples. It is stable and provides an excellent recovery for serum samples, thus, potentially it can be useful in this field due to its low energy consumption, enzyme free assay, fast response time, better selectivity and sensitivity.

The authors describe the preparation of gold nanoparticles (AuNPs) coated with poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol) (Pluronic F-108) by reducing Au 3+ to Au 0 using curcumin, a natural and non-toxic food spice, in water of pH ~7 in the presence of F-108 and Ag + ion. The coated AuNPs display strong resonance Rayleigh scattering (RRS) and fluorescence that results from the functionalization of the gold surface with curcumin and Pluronic F-108. The molar mass of Pluronic F-108 affects the particle size of the AuNPs formed, and small AuNPs are formed when using low molar weight F-108 that was purified by centrifugation or dialysis. The coated AuNPs were employed in an optical method for the determination of uric acid. The combination of uric acid with the AuNPs boosts both the RRS signal and the fluorescence of the AuNPs. However, higher concentrations of uric acid shift the fluorescence peak to shorter wavelengths. The method is simple, and fluorescence, best measured at excitation/emission wavelengths of 425/534 nm, increases linearly in the 50 μM to 50 mM uric acid concentration range, with a 0.14 μM detection limit which is lower than reported for other methods in the literature. Graphical abstract Pluronic F-108 capped gold nanoparticles prepared by reducing Au 3+ to Au 0 using curcumin can estimate uric acid in 50 μM to 50 mM concentration range.

Tuning optical properties by controlling size and shape of the metallic nanoparticles has been of great interest to design novel bio-sensing techniques. Here, as a first example we illustrate that resonance Rayleigh scattering (RRS) signal of Au nanoparticles (NPs) can be amplified >10-fold by growing into Au nanowires (NWs). These thin and long NWs of ~20-25nm diameter and >1µm length can be achieved by suitably manipulating the temperature during green synthesis using curcumin. Interestingly, mixture of Au NWs and NPs or shorter NWs gives a moderate increase in RRS signal suggesting formation of longer NWs is crucial for optimal enhancement of RRS signal. Curcumin along with CTAB act as capping and stabilizing agent for Au NWs/NPs in different temperatures, which is confirmed by XRD, TGA, DSC, EDX and FT-IR data. This amplified RRS signal of Au NWs has been employed to design a new optical biosensor for α-tocopherol (α-TOH), which is among the most biologically active form of vitamin E. Association of α-TOH with Au NWs further enhances the RRS signal of Au NWs, ~10 fold through non-covalent interaction. No interference from other antioxidant substances like ascorbic acid and 6-O-Palmitoyl-L-ascorbic acid is observed. The sensing method is simple, fast and offers remarkable linear dynamic ranges, 12.8-1004µmolL -1 , which is larger than reported values. The detection limit for α-TOH estimation has been found to be 50nmolL -1 . The biosensor is found to be stable both in the absence and presence of α-TOH and provides an excellent recovery for synthetic samples., (Copyright © 2017 Elsevier B.V. All rights reserved.)