98 results on '"Kupsky WJ"'
Search Results
2. Severe proximal myopathy and mononeuritis multiplex in rheumatoid arthritis: manifestations of rheumatoid vasculitis.
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Chatterjee S, Kupsky WJ, Chatterjee, Soumya, and Kupsky, William Joseph
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- 2005
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3. Central Nervous System Tumor With BCL6 Corepressor Internal Tandem Duplication: Treatment Course of a Long-term Survivor.
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Bakkar M, Altinok D, Kupsky WJ, Marupudi NI, Chiang J, and Gorsi HS
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- Male, Humans, Child, Proto-Oncogene Proteins genetics, Neoplasm Recurrence, Local, Transcription Factors, Co-Repressor Proteins, Proto-Oncogene Proteins c-bcl-6 genetics, Repressor Proteins genetics, Central Nervous System Neoplasms diagnostic imaging, Central Nervous System Neoplasms genetics, Central Nervous System Neoplasms therapy
- Abstract
Central nervous system (CNS) tumor with BCL6 corepressor (BCOR) internal tandem duplication (ITD) is a newly described CNS tumor, characterized by in-frame ITDs of the BCOR gene. There is no standard practice regarding the management of this tumor. We report the clinical course of a 6-year-old boy who presented to the hospital with worsening headaches. Computed tomography scan showed a large right-sided parietal supratentorial mass and brain magnetic resonance imaging confirmed a 6×8×6.7 cm lobulated, solid but heterogeneous mass in the right parieto-occipital region. While initial pathology suggested a WHO grade 3 anaplastic meningioma, additional investigation with molecular analysis confirmed the diagnosis of high-grade neuroepithelial tumor with BCOR exon 15 ITD. This diagnosis was renamed CNS tumor with BCOR ITD in the 2021 WHO CNS tumor classification. The patient received 54 Gy of focal radiation and has no evidence of disease recurrence after 48 months from the end of treatment. As this is a newly discovered entity with only a few previous reports in the scientific literature, this report presents a unique treatment for this CNS tumor compared with those previously described., Competing Interests: The authors declare no conflict of interest., (Copyright © 2023 Wolters Kluwer Health, Inc. All rights reserved.)
- Published
- 2023
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4. Pineal Parenchymal Tumor of Intermediate Differentiation and DICER1 Syndrome: A Case Report.
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Gupte A, Sood S, Kupsky WJ, Altinok D, Miller S, Roy S, and Bhambhani K
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- Humans, Female, Adolescent, Syndrome, Pedigree, Pinealoma diagnostic imaging, Pinealoma genetics, Pinealoma pathology, Brain Neoplasms diagnostic imaging, Brain Neoplasms genetics, Brain Neoplasms pathology, Pineal Gland diagnostic imaging, Pineal Gland pathology, Ribonuclease III genetics, DEAD-box RNA Helicases genetics, Ciliary Body pathology, Uveal Neoplasms genetics, Uveal Neoplasms pathology, Genetic Predisposition to Disease
- Abstract
DICER1 syndrome is a rare inherited tumor predisposition syndrome associated with an increased risk for several malignant and benign tumors. We present a patient with pineal parenchymal tumor of intermediate differentiation who was found to have a germline pathogenic variant in DICER1 gene. Pineoblastoma is a known DICER1-related tumor; however, the association between pineal parenchymal tumor of intermediate differentiation and DICER1 mutation is rare with only 1 recent large molecular study that has reported this association. This report adds to the evolving tumor spectrum of DICER1 and highlights the importance of molecular evaluation of pediatric brain tumors, for both therapeutic decisions and long-term surveillance., Competing Interests: The authors declare no conflict of interest., (Copyright © 2022 Wolters Kluwer Health, Inc. All rights reserved.)
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- 2023
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5. Histopathology of new-onset refractory status epilepticus (NORSE) in adults.
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Suchdev K, Kupsky WJ, Mittal S, and Shah AK
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- Acute Disease, Adult, Humans, Status Epilepticus therapy
- Abstract
Objective: new-onset refractory status epilepticus (NORSE) is defined as de novo refractory seizures occurring in previously healthy adults, without a clear underlying etiology. Due to refractory seizures and insufficient understanding of pathophysiology, management of these patients remains challenging and often leads to poor clinical outcomes. Various infectious and autoimmune mechanisms have been proposed but have not been validated and a large number of patients are thus labeled 'cryptogenic'. Moreover, histopathological findings have rarely been described in NORSE and are usually autopsy evaluations. In this paper, we describe the clinical correlates and histopathological findings in patients presenting with NORSE., Methods: A case series of five patients with NORSE who underwent neurosurgical intervention and had histopathological examination during their acute clinical course., Results: In all patients,status epileptics was refractory to treatment with antiseizure drugs (ASDs) and anesthetic agents. Autoimmune work-up revealed elevated titer of anti-GAD antibody in one patient but was unremarkable in others. Empiric use of immunomodulation therapy in three patients did not lead to cessation of status epilepticus (SE). Due to failure of prolonged medical management, three patients underwent palliative surgery for resection of epileptogenic tissue whereas the other two had diagnostic brain biopsy. Histopathology obtained during biopsy revealed evidence of vasculitis in one and necrotizing vasculopathy in another. The patient with anti-GAD antibodies had evidence of lymphocytic infiltration in limbic structures. The remaining two had nonspecific histopathological findings., Significance: Although our findings are limited by a small number of patients, it adds to the growing premise of NORSE being related to an underlying autoimmune process. Additional studies, especially with histopathological data are needed to better understand this devastating disorder., (Copyright © 2021 British Epilepsy Association. Published by Elsevier Ltd. All rights reserved.)
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- 2021
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6. Successful Surgical Treatment of Refractory Status Epilepticus in a 12-Day-Old Infant.
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Peterson C, Garling RJ, Asano E, Kupsky WJ, Set K, Agarwal R, and Sood S
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- Drug Resistant Epilepsy physiopathology, Electroencephalography, Frontal Lobe physiopathology, Humans, Infant, Newborn, Magnetic Resonance Imaging, Male, Status Epilepticus physiopathology, Drug Resistant Epilepsy surgery, Frontal Lobe surgery, Status Epilepticus surgery
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- 2019
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7. Investigation of the aryl hydrocarbon receptor and the intrinsic tumoral component of the kynurenine pathway of tryptophan metabolism in primary brain tumors.
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Guastella AR, Michelhaugh SK, Klinger NV, Fadel HA, Kiousis S, Ali-Fehmi R, Kupsky WJ, Juhász C, and Mittal S
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- Antineoplastic Agents therapeutic use, Brain Neoplasms drug therapy, Brain Neoplasms pathology, Cell Line, Tumor, Gene Expression Regulation, Neoplastic, Glioma drug therapy, Glioma pathology, Humans, Meningeal Neoplasms drug therapy, Meningeal Neoplasms pathology, Meningioma drug therapy, Meningioma pathology, Neoplasm Grading, Tryptophan metabolism, Brain Neoplasms metabolism, Glioma metabolism, Kynurenine metabolism, Meningeal Neoplasms metabolism, Meningioma metabolism, Receptors, Aryl Hydrocarbon metabolism
- Abstract
Introduction: There is mounting evidence supporting the role of tryptophan metabolism via the kynurenine pathway (KP) in the pathogenesis of primary brain tumors. Under normal physiological conditions, the KP is the major catabolic pathway for the essential amino acid tryptophan. However, in cancer cells, the KP becomes dysregulated, depletes local tryptophan, and contributes to an immunosuppressive tumor microenvironment., Methods: We examined the protein expression levels (in 73 gliomas and 48 meningiomas) of the KP rate-limiting enzymes indoleamine 2,3-dioxygenase (IDO) 1, IDO2, and tryptophan 2,3-dioxygenase (TDO2), as well as, the aryl hydrocarbon receptor (AhR), a carcinogenic transcription factor activated by KP metabolites. In addition, we utilized commercially available small-molecules to pharmacologically modulate IDO1, IDO2, TDO2, and AhR in patient-derived glioma and meningioma cell lines (n = 9 each)., Results: We observed a positive trend between the grade of the tumor and the average immunohistochemical staining score for IDO1, IDO2, and TDO2, with TDO2 displaying the strongest immunostaining. AhR immunostaining was present in all grades of gliomas and meningiomas, with the greatest staining intensity noted in glioblastomas. Immunocytochemical staining showed a positive trend between nuclear localization of AhR and histologic grade in both gliomas and meningiomas, suggesting increased AhR activation with higher tumor grade. Unlike enzyme inhibition, AhR antagonism markedly diminished patient-derived tumor cell viability, regardless of tumor type or grade, following in vitro drug treatments., Conclusions: Collectively, these results suggest that AhR may offer a novel and robust therapeutic target for a patient population with highly limited treatment options.
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- 2018
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8. Acute Resective Surgery for the Treatment of Refractory Status Epilepticus.
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Basha MM, Suchdev K, Dhakar M, Kupsky WJ, Mittal S, and Shah AK
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- Adult, Aged, Drug Resistant Epilepsy physiopathology, Electrocorticography, Female, Humans, Male, Middle Aged, Retrospective Studies, Status Epilepticus physiopathology, Young Adult, Drug Resistant Epilepsy surgery, Outcome Assessment, Health Care, Status Epilepticus surgery
- Abstract
Background: To identify the role of acute surgical intervention in the treatment of refractory status epilepticus (RSE)., Methods: Retrospective review of consecutive patients who underwent epilepsy surgery from 2006 to 2015 was done to identify cases where acute surgical intervention was employed for the treatment of RSE. In addition, the adult and pediatric RSE literature was reviewed for reports of surgical treatment of RSE., Results: Nine patients, aged 20-68 years, with various etiologies were identified to have undergone acute surgical resection for the treatment of RSE, aided by electrocorticography. Patients required aggressive medical therapy with antiepileptic drugs and intravenous anesthetic drugs for 10-54 days and underwent extensive neurodiagnostic testing prior to resective surgery. Eight out of nine patients survived and five patients were seizure-free at the last follow-up. The literature revealed 13 adult and 48 pediatric cases where adequate historical detail was available for review and comparison., Conclusions: We present the largest cohort of consecutive adult patients who underwent resective surgery in the setting of RSE. We also reveal that surgery can be efficacious in aborting status and in some can lead to long-term seizure freedom. Acute surgical intervention is a viable option in prolonged RSE and proper evaluation for such intervention should be conducted, although the timing and type of surgical intervention remain poorly defined.
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- 2017
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9. GNAQ Mutation in the Venous Vascular Malformation and Underlying Brain Tissue in Sturge-Weber Syndrome.
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Sundaram SK, Michelhaugh SK, Klinger NV, Kupsky WJ, Sood S, Chugani HT, Mittal S, and Juhász C
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- Brain diagnostic imaging, Brain surgery, Child, Child, Preschool, Drug Resistant Epilepsy diagnostic imaging, Drug Resistant Epilepsy genetics, Drug Resistant Epilepsy pathology, Drug Resistant Epilepsy surgery, Female, Humans, Infant, Male, Meninges diagnostic imaging, Meninges pathology, Meninges surgery, Mutation, Phenotype, Sturge-Weber Syndrome diagnostic imaging, Sturge-Weber Syndrome surgery, Vascular Malformations diagnostic imaging, Vascular Malformations surgery, White Matter diagnostic imaging, White Matter pathology, White Matter surgery, Brain pathology, GTP-Binding Protein alpha Subunits, Gq-G11 genetics, Sturge-Weber Syndrome genetics, Sturge-Weber Syndrome pathology, Vascular Malformations genetics, Vascular Malformations pathology
- Abstract
Competing Interests: Conflict of Interest: The authors report no conflict of interest.
- Published
- 2017
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10. Tryptophan PET Imaging of the Kynurenine Pathway in Patient-Derived Xenograft Models of Glioblastoma.
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Guastella AR, Michelhaugh SK, Klinger NV, Kupsky WJ, Polin LA, Muzik O, Juhász C, and Mittal S
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- Aged, Animals, Biosynthetic Pathways, Brain Neoplasms diagnostic imaging, Brain Neoplasms pathology, Carbon Radioisotopes chemistry, Cell Line, Tumor, Female, Glioblastoma diagnostic imaging, Glioblastoma pathology, Humans, Male, Mice, Middle Aged, Neoplasm Transplantation, Tryptophan chemistry, Brain Neoplasms metabolism, Glioblastoma metabolism, Kynurenine metabolism, Molecular Imaging methods, Positron-Emission Tomography methods, Tryptophan pharmacokinetics
- Abstract
Increasing evidence demonstrates the immunosuppressive kynurenine pathway's (KP) role in the pathophysiology of human gliomas. To study the KP in vivo, we used the noninvasive molecular imaging tracer α-[(11)C]-methyl-l-tryptophan (AMT). The AMT-positron emission tomography (PET) has shown high uptake in high-grade gliomas and predicted survival in patients with recurrent glioblastoma (GBM). We generated patient-derived xenograft (PDX) models from dissociated cells, or tumor fragments, from 5 patients with GBM. Mice bearing subcutaneous tumors were imaged with AMT-PET, and tumors were analyzed to detect the KP enzymes indoleamine 2,3-dioxygenase (IDO) 1, IDO2, tryptophan 2,3-dioxygenase, kynureninase, and kynurenine 3-monooxygenase. Overall, PET imaging showed robust tumoral AMT uptake in PDX mice with prolonged tracer accumulation over 60 minutes, consistent with AMT trapping seen in humans. Immunostained tumor tissues demonstrated positive detection of multiple KP enzymes. Furthermore, intracranial implantation of GBM cells was performed with imaging at both 9 and 14 days postimplant, with a marked increase in AMT uptake at 14 days and a corresponding high level of tissue immunostaining for KP enzymes. These results indicate that our PDX mouse models recapitulate human GBM, including aberrant tryptophan metabolism, and offer an in vivo system for development of targeted therapeutics for patients with GBM., (© The Author(s) 2016.)
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- 2016
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11. Surgical treatment for refractory epileptic spasms: The Detroit series.
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Chugani HT, Ilyas M, Kumar A, Juhász C, Kupsky WJ, Sood S, and Asano E
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- Adolescent, Brain pathology, Child, Child, Preschool, Drug Resistant Epilepsy pathology, Electrocorticography, Female, Humans, Infant, Magnetic Resonance Imaging, Male, Positron-Emission Tomography, Postoperative Complications etiology, Spasms, Infantile pathology, Treatment Outcome, Young Adult, Drug Resistant Epilepsy surgery, Hemispherectomy methods, Spasms, Infantile surgery
- Abstract
Objective: We reviewed our experience of surgery for epileptic spasms (ES) with or without history of infantile spasms., Methods: Data were reviewed from 65 (33 male) patients with ES who underwent surgery between 1993 and 2014; palliative cases were excluded., Results: Mean age at surgery was 5.1 (range 0.2-19) years, with mean postsurgical follow-up of 45.3 (6-120) months. Mean number of anticonvulsants used preoperatively was 4.2 (2-8), which decreased to 1.2 (0-4) postoperatively (p < 0.0001). Total hemispherectomy was the most commonly performed surgery (n = 20), followed by subtotal hemispherectomy (n = 17), multilobar resection (n = 13), lobectomy (n = 7), tuberectomy (n = 6), and lobectomy + tuberectomy (n = 2), with International League Against Epilepsy (ILAE) class I outcome in 20, 10, 7, 6, 3, and 0 patients, respectively (total 46/65 (71%); 22 off medication). Shorter duration of epilepsy (p = 0.022) and presence of magnetic resonance imaging (MRI) lesion (p = 0.026) were independently associated with class I outcome. Of 34 patients operated <3 years after seizure onset, 30 (88%) achieved class I outcome. Thirty-seven (79%) of 47 patients with lesional MRI had class-I outcome, whereas 9 (50%) of 18 with normal MRI had class I outcome. Positron emission tomography (PET) scan was abnormal in almost all patients [61 (97%) of 63 with lateralizing/localizing findings in 56 (92%) of 61 patients, thus helping in surgical decision making and guiding subdural grid placements, particularly in patients with nonlesional MRI. Fifteen patients had postoperative complications, mostly minor., Significance: Curative epilepsy surgery in ES patients, with or without history of infantile spasms, is best accomplished at an early age and in those patients with lesional abnormalities on MRI with electroencephalography (EEG) concordance. Good outcomes can be achieved even when there is no MRI lesion but positive PET localization., (Wiley Periodicals, Inc. © 2015 International League Against Epilepsy.)
- Published
- 2015
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12. Molecular imaging correlates of tryptophan metabolism via the kynurenine pathway in human meningiomas.
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Bosnyák E, Kamson DO, Guastella AR, Varadarajan K, Robinette NL, Kupsky WJ, Muzik O, Michelhaugh SK, Mittal S, and Juhász C
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- Adolescent, Adult, Aged, Aged, 80 and over, Brain metabolism, Brain pathology, Child, Female, Glioma diagnostic imaging, Glioma metabolism, Glioma pathology, Humans, Male, Meningeal Neoplasms diagnostic imaging, Meningioma diagnostic imaging, Middle Aged, Neoplasm Grading, Positron-Emission Tomography, Signal Transduction, Tryptophan analogs & derivatives, Young Adult, Kynurenine metabolism, Meningeal Neoplasms metabolism, Meningeal Neoplasms pathology, Meningioma metabolism, Meningioma pathology, Tryptophan metabolism
- Abstract
Background: Increased tryptophan metabolism via the kynurenine pathway (KP) is a key mechanism of tumoral immune suppression in gliomas. However, details of tryptophan metabolism in meningiomas have not been elucidated. In this study, we evaluated in vivo tryptophan metabolism in meningiomas and compared it with gliomas using α-[(11)C]-methyl-L-tryptophan (AMT)-PET. We also explored expression patterns of KP enzymes in resected meningiomas., Methods: Forty-seven patients with MRI-detected meningioma (n = 16) and glioma (n = 31) underwent presurgical AMT-PET scanning. Tumoral AMT uptake and tracer kinetic parameters (including K and k3' evaluating unidirectional uptake and trapping, respectively) were measured, correlated with meningioma grade, and compared between meningiomas and gliomas. Patterns of KP enzyme expression were assessed by immunohistochemistry in all meningiomas., Results: Meningioma grade showed a positive correlation with AMT k3' tumor/cortex ratio (r = 0.75, P = .003), and this PET parameter distinguished grade I from grade II/III meningiomas with 92% accuracy. Kinetic AMT parameters could differentiate meningiomas from both low-grade gliomas (97% accuracy by k3' ratios) and high-grade gliomas (83% accuracy by K ratios). Among 3 initial KP enzymes (indoleamine 2,3-dioxygenase 1/2, and tryptophan 2,3-dioxygenase 2 [TDO2]), TDO2 showed the strongest immunostaining, particularly in grade I meningiomas. TDO2 also showed a strong negative correlation with AMT k3' ratios (P = .001)., Conclusions: PET imaging of tryptophan metabolism can provide quantitative imaging markers for differentiating grade I from grade II/III meningiomas. TDO2 may be an important driver of in vivo tryptophan metabolism in these tumors. These results can have implications for pharmacological targeting of the KP in meningiomas., (© The Author(s) 2015. Published by Oxford University Press on behalf of the Society for Neuro-Oncology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
- Published
- 2015
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13. Multi-modal imaging of tumor cellularity and Tryptophan metabolism in human Gliomas.
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Jeong JW, Juhász C, Mittal S, Bosnyák E, Kamson DO, Barger GR, Robinette NL, Kupsky WJ, and Chugani DC
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- Adolescent, Adult, Aged, Cellulase, Female, Humans, Immunohistochemistry, Male, ROC Curve, Tryptophan, Brain Neoplasms metabolism, Brain Neoplasms pathology, Diffusion Tensor Imaging, Glioma metabolism, Glioma pathology, Positron-Emission Tomography
- Abstract
Background: To assess gliomas using image-based estimation of cellularity, we utilized isotropic diffusion spectrum imaging (IDSI) on clinically feasible diffusion tensor imaging (DTI) and compared it with amino acid uptake measured by α[(11)C]methyl-L-tryptophan positron emission tomography (AMT-PET)., Methods: In 10 patients with a newly-diagnosed glioma, metabolically active tumor regions were defined in both FLAIR hyperintense areas and based on increased uptake on AMT-PET. A recently developed independent component analysis with a ball and stick model was extended to perform IDSI in clinical DTI data. In tumor regions, IDSI was used to define tumor cellularity which was compared between low and high grade glioma and correlated with the glioma proliferative index., Results: The IDSI-derived cellularity values were elevated in both FLAIR and AMT-PET-derived regions of high-grade gliomas. ROC curve analysis found that the IDSI-derived cellularity can provide good differentiation of low-grade from high-grade gliomas (accuracy/sensitivity/specificity of 0.80/0.80/0.80). . Both apparent diffusion coefficient (ADC) and IDSI-derived cellularity showed a significant correlation with the glioma proliferative index (based on Ki-67 labeling; R = 0.95, p < 0.001), which was particularly strong when the tumor regions were confined to areas with high tryptophan uptake excluding areas with peritumoral edema., Conclusion: IDSI-MRI combined with AMT-PET may provide a multi-modal imaging tool to enhance pretreatment assessment of human gliomas by evaluating tumor cellularity and differentiate low-grade form high-grade gliomas.
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- 2015
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14. Antenatal brain injury in third trimester neonates with severe congenital anomalies: an autopsy study.
- Author
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Jacques SM, Kupsky WJ, and Qureshi F
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- Adolescent, Adult, Autopsy, Brain Diseases mortality, Brain Diseases pathology, Congenital Abnormalities physiopathology, Female, Fetal Blood chemistry, Fetal Diseases mortality, Fetal Diseases pathology, Humans, Hydrogen-Ion Concentration, Infant, Newborn, Necrosis, Neurons pathology, Placenta pathology, Pregnancy, Pregnancy Trimester, Third, Brain Diseases etiology, Congenital Abnormalities embryology, Fetal Diseases etiology
- Abstract
Objective: With advances in therapy, more neonates with severe congenital anomalies are surviving, albeit some with neurologic disorders, possibly related to antenatal low brain blood flow. This autopsy series reports antenatal brain injury in neonates expiring due to severe anomalies, and provides correlation with umbilical cord blood gas and acid-base analysis., Methods: We identified autopsies of third trimester neonates expiring shortly following delivery due to severe anomalies or malformations. Brain injury classified as "older" included periventricular leukomalacia, gliosis and karyorrhectic neurons, and "recent" included red neurons and reactive glial changes., Results: We identified 22 cases (nine term, 13 preterm). 16 (73%) had brain injury, including 11 with older injury. Cord arterial blood was analyzed in 17, and six had pH <7 or base deficit >12 mmol/L. Four out of 5 (80%) neonates with neuronal necrosis compared to two out of 12 (17%) without had a pH <7 or base deficit >12 mmol/L (p = 0.03). Five out of nine (56%) neonates with white matter injury compared to one out of 8 (13%) without had pH <7 or base deficit >12 mmol/L (p = NS)., Conclusions: Antenatal brain injury is frequent in neonates with severe congenital anomalies. Severely abnormal cord blood analysis results correlate significantly with neuronal necrosis and show a trend toward white matter injury; however, the absence of these abnormal results does not preclude the presence of brain injury.
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- 2015
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15. Acute thymic involution in unexplained third trimester stillbirth: frequency, grade, and correlation with neuropathologic injury.
- Author
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Jacques SM, Kupsky WJ, and Qureshi F
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- Female, Humans, Male, Pregnancy, Pregnancy Trimester, Third, Brain pathology, Stillbirth, Thymus Gland pathology
- Abstract
Many 3rd-trimester stillbirths are unexplained, including the time course of the illness. Histologic acute thymic involution (ATI), when graded, correlates with duration of acute illness (grade 0, <12 hours; grade 4, >72 hours). Histologic brain injury is also common in stillbirth. We investigated ATI in unexplained stillbirth and correlated it with neuropathologic injury by identifying 58 autopsies of unexplained, 3rd-trimester stillborns (preterm, n = 24; term, n = 34) that included brain examination and graded ATI from 0 (resting state) to 4 (pronounced lymphodepletion). Gray matter injury (GMI) and white matter injury (WMI) were classified as older, recent, or absent, and ATI was correlated with GMI, WMI, thymic weight, and clinical data. Nine cases (16%) had ATI grade 0-1; 19 (33%), grade 2; 24 (41%), grade 3; and 6 (10%), grade 4. Older GMI and WMI were present in 39 (67%) and 10 (17%) stillborns, respectively. Higher ATI grade correlated significantly with older GMI (P < 0.001) and WMI (P = 0.014). The ATI grade was higher in the small-for-gestational stillborns compared with the appropriate- or large-for-gestational stillborns (P = 0.017) but did not correlate significantly with gestational age or other clinical or demographic factors evaluated. The ATI grades 2-4 were found in 84% of the stillborns, consistent with onset of acute illness between 24 and >72 hours before demise. Higher ATI grade correlated significantly with older brain injury, suggesting similar time of onset and shared underlying pathophysiologic events, the specific nature of which remains unclear.
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- 2015
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16. "Subtotal" hemispherectomy in children with intractable focal epilepsy.
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Chugani HT, Asano E, Juhász C, Kumar A, Kupsky WJ, and Sood S
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- Adolescent, Child, Child, Preschool, Electroencephalography, Epilepsies, Partial diagnostic imaging, Epilepsies, Partial pathology, Female, Fluorodeoxyglucose F18, Humans, Infant, Longitudinal Studies, Magnetic Resonance Imaging, Male, Positron-Emission Tomography, Retrospective Studies, Treatment Outcome, Epilepsies, Partial surgery, Hemispherectomy methods
- Abstract
Objective: Cortical resections in epilepsy surgery tend to be larger in children, compared to adults, partly due to underlying pathology. Some children show unilateral multifocal seizure onsets involving much of the hemisphere. If there were a significant hemiparesis present, hemispherectomy would be the procedure of choice. Otherwise, it is preferable to spare the primary sensorimotor cortex. We report the results of "subtotal" hemispherectomy in 23 children., Methods: All children (ages 1 year and 4 months to 14 years and 2 months) were operated on between 2001 and 2013 at Children's Hospital of Michigan (Detroit). Patients were evaluated with scalp video-electroencephalography (EEG), magnetic resonance imaging (MRI), (18) F-fluorodeoxyglucose-positron emission tomography (FDG-PET) scans, and neuropsychological assessments when applicable. Subsequently, each case was discussed in a multidisciplinary epilepsy surgery conference, and a consensus was reached pertaining to candidacy for surgery and optimum surgical approach. The actual extent of resection was based on the results from subdural electrocorticography (ECoG) monitoring. The surgical outcome is based on International League Against Epilepsy (ILAE) classification (class 1-6)., Results: Among the 23 patients, 11 had epileptic spasms as their major seizure type; these were associated with focal seizures in 3 children. MRI showed focal abnormalities in 12 children. FDG-PET was abnormal in all but one subject. All except two children underwent chronic subdural ECoG. Multiple subpial transections were performed over the sensorimotor cortex in three subjects. On histopathology, various malformations were seen in 9 subjects; the remainder showed gliosis alone (n = 12), porencephaly (n = 1), and gliosis with microglial activation (n = 1). Follow-up ranged from 13 to 157 months (mean = 65 months). Outcomes consisted of class 1 (n = 17, 74%), class 2 (n = 2), class 3 (n = 1), class 4 (n = 1), and class 5 (n = 2)., Significance: Extensive unilateral resections sparing only sensorimotor cortex can be performed with excellent results in seizure control. Even with the presence of widespread unilateral epileptogenicity or anatomic/functional imaging abnormalities, complete hemispherectomy can often be avoided, particularly when there is little hemiparesis., (Wiley Periodicals, Inc. © 2014 International League Against Epilepsy.)
- Published
- 2014
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17. Spontaneous intratumoral infarction--an unusual evolution of a falcine meningioma.
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Jain A, Hoeprich M, Mittal M, Kupsky WJ, and Mittal S
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- Brain Neoplasms diagnosis, Diagnosis, Differential, Female, Humans, Infarction diagnosis, Magnetic Resonance Imaging methods, Meningeal Neoplasms diagnosis, Meningioma diagnosis, Middle Aged, Necrosis pathology, Supratentorial Neoplasms diagnosis, Supratentorial Neoplasms pathology, Brain Neoplasms pathology, Infarction pathology, Meningeal Neoplasms pathology, Meningioma pathology
- Abstract
Objective: We report the incidence of spontaneous infarction of a falcine meningioma without preceding hemorrhage and shed light on the relation between intratumoral necrosis and hemorrhage., Clinical Presentation: A 50 year-old woman presented with recurrent headaches and was found to harbor a falcine meningioma. The patient elected to observe the mass and 13 months later she developed new neurological deficits. Prior to scheduled resection, she presented with abdominal pain and underwent emergent laparoscopic cholecystectomy. Two days after the procedure, she developed sudden right hemiparesis associated with severe headache. MRI of the brain showed an intratumoral wedge-shaped hypointense area with significant peritumoral edema. The patient was started on high-dose corticosteroids with considerable improvement in strength., Intervention: The patient underwent a complete resection of the tumor with no new neurological deficits post-operatively. Histopathological analysis confirmed a WHO grade II atypical meningioma with extensive necrosis without hemorrhage., Conclusion: This case highlights that tumor infarction, although rare, should be in the differential diagnosis of patients with meningiomas presenting with new neurological deficits. When this condition is recognized and treated in timely manner with high-dose corticosteroids and surgical resection, patients can have favorable long-term outcomes.
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- 2014
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18. Increased tryptophan uptake on PET has strong independent prognostic value in patients with a previously treated high-grade glioma.
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Kamson DO, Mittal S, Robinette NL, Muzik O, Kupsky WJ, Barger GR, and Juhász C
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- Adult, Aged, Aged, 80 and over, Brain Neoplasms diagnostic imaging, Brain Neoplasms metabolism, Brain Neoplasms mortality, Carbon Radioisotopes, Female, Glioma diagnostic imaging, Glioma metabolism, Glioma mortality, Humans, Male, Middle Aged, Neoplasm Recurrence, Local diagnostic imaging, Neoplasm Recurrence, Local metabolism, Neoplasm Recurrence, Local mortality, Prognosis, Survival Analysis, Tryptophan analogs & derivatives, Brain Neoplasms diagnosis, Glioma diagnosis, Neoplasm Recurrence, Local diagnosis, Positron-Emission Tomography, Tryptophan pharmacokinetics
- Abstract
Background: Previously, we demonstrated the high accuracy of alpha-[(11)C]methyl-L-tryptophan (AMT) PET for differentiating recurrent gliomas from radiation injury. The present study evaluated the prognostic value of increased AMT uptake in patients with previously treated high-grade glioma., Methods: AMT-PET was performed in 39 patients with suspected recurrence of World Health Organization grades III-IV glioma following surgical resection, radiation, and chemotherapy. Mean and maximum standardized uptake values (SUVs) and unidirectional AMT uptake (K) were measured in brain regions suspicious for tumor and compared with the contralateral cortex (ie, background). Optimal cutoff thresholds for 1-year survival prediction were determined for each AMT parameter and used for calculating the prognostic value of high (above threshold) versus low (below threshold) values for post-PET overall survival (OS)., Results: In univariate analyses, 1-year survival was strongly associated with 3 AMT parameters (SUVmax, SUVmean, and tumor-to-background K-ratio; odds ratios: 21.3-25.6; P ≤ .001) and with recent change in MRI contrast enhancement (odds ratio: 14.7; P = .02). Median OS was 876 days in the low- versus 177 days in the high-AMT groups (log-rank P < .001). In multivariate analyses, all 3 AMT parameters remained strong predictors of survival: high AMT values were associated with unfavorable 1-year survival (binary regression P ≤ .003) and shorter overall survival in the whole group (Cox regression hazard ratios: 5.3-10.0) and in patients with recent enhancement change on MRI as well (hazard ratios: 7.0-9.3; P ≤ .001)., Conclusion: Increased AMT uptake on PET is highly prognostic for 1-year and overall survival, independent of MRI contrast enhancement and other prognostic factors in patients with a previously treated high-grade glioma., (© The Author(s) 2014. Published by Oxford University Press on behalf of the Society for Neuro-Oncology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
- Published
- 2014
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19. Third trimester fetal demise occurring at time of delivery: correlation of autopsy findings and placental pathology with emphasis on antenatal central nervous system injury.
- Author
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Jacques SM, Kupsky WJ, and Qureshi F
- Subjects
- Adult, Delivery, Obstetric, Female, Humans, Male, Pregnancy, Pregnancy Trimester, Third, Young Adult, Brain pathology, Brain Injuries, Placenta pathology, Stillbirth
- Abstract
Objective: Third trimester fetal deaths occurring in the hospital at the time of delivery are unusual. We report an autopsy series of such cases with emphasis on neuropathological injury and other lesions predating delivery., Methods: We identified autopsies performed on third trimester fetuses documented to be alive shortly before delivery, but that expired during, or very close to, time of delivery, and we correlate autopsy and placental findings. Fetuses with major congenital anomalies were excluded., Results: Ten cases were identified (6 term, 4 preterm). All were delivered by cesarean section and had attempted resuscitation. Established or recent brain injury was identified in 9 of 10 cases, including 3 with established neuronal damage and 1 with periventricular leukomalacia. Additional autopsy findings included thymic involution in eight (five mild; three severe), myocardial infarcts in two; intrathoracic petechiae in five, and ascites or pleural or pericardial effusions in six. Severe thymic involution and myocardial infarcts correlated with established brain injury. Placental lesions adaptive to decreased oxygenation (increased nucleated red blood cells or villous hypervascularity) were seen in five cases and correlated with established brain injury. Acute chorioamnionitis with funisitis was present in one, and chronic inflammatory placental lesions were present in six., Conclusions: These findings indicate brain injury predated the time period immediately before delivery in 9 of 10 fetuses, and in the fetuses with established brain injury the onset of acute illness was possibly >72 h before delivery.
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- 2014
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20. Histopathological evidence that hippocampal atrophy following status epilepticus is a result of neuronal necrosis.
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Kumar G, Mittal S, Moudgil SS, Kupsky WJ, and Shah AK
- Subjects
- Adult, Edema, Female, Humans, Nerve Degeneration complications, Neuroimaging, Atrophy, Hippocampus pathology, Necrosis, Nerve Degeneration pathology, Status Epilepticus pathology
- Abstract
Medial temporal lobe epilepsy is commonly associated with hippocampal atrophy on MRI and hippocampal sclerosis on histopathological examination of surgically-resected specimens. Likewise, it is well-established that prolonged seizures and status epilepticus can lead to hippocampal edema as noted on MRI. In this paper, the authors present an unusual patient with prolonged refractory status epilepticus, due to limbic encephalitis associated with anti-GAD antibody, who underwent palliative epilepsy surgery. Bilateral hippocampal edema was noted on preoperative MRI. Histologic evaluation confirmed presence of acute necrosis and neuronal loss in the left hippocampal formation. Follow-up MRI several months after surgery demonstrated severe atrophy of the contralateral right hippocampus. This is the first clear histopathological evidence that hippocampal atrophy following status epilepticus is the result of acute neuronal necrosis and cell loss., (© 2013 Elsevier B.V. All rights reserved.)
- Published
- 2013
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21. Synchronous occurrence of paraganglioma of the glomus jugulare and olfactory groove meningioma.
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Mittal S, Monsell EM, Narayanan S, Kupsky WJ, Guthikonda M, and Mittal S
- Subjects
- Aged, Female, Glomus Jugulare Tumor surgery, Humans, Meningeal Neoplasms surgery, Meningioma surgery, Neoplasms, Multiple Primary surgery, Glomus Jugulare Tumor pathology, Meningeal Neoplasms pathology, Meningioma pathology, Neoplasms, Multiple Primary pathology
- Published
- 2013
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22. Differentiation of glioblastomas from metastatic brain tumors by tryptophan uptake and kinetic analysis: a positron emission tomographic study with magnetic resonance imaging comparison.
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Kamson DO, Mittal S, Buth A, Muzik O, Kupsky WJ, Robinette NL, Barger GR, and Juhász C
- Subjects
- Adult, Aged, Aged, 80 and over, Breast Neoplasms diagnostic imaging, Breast Neoplasms secondary, Diagnosis, Differential, Female, Humans, Lung Neoplasms diagnostic imaging, Lung Neoplasms secondary, Male, Middle Aged, Multimodal Imaging, Neoplasm Metastasis, Tryptophan pharmacokinetics, Brain Neoplasms diagnostic imaging, Brain Neoplasms pathology, Glioblastoma diagnostic imaging, Glioblastoma pathology, Magnetic Resonance Imaging, Positron-Emission Tomography, Tryptophan analogs & derivatives
- Abstract
Differentiating high-grade gliomas from solitary brain metastases is often difficult by conventional magnetic resonance imaging (MRI); molecular imaging may facilitate such discrimination. We tested the accuracy of α[11C]methyl-l-tryptophan (AMT)-positron emission tomography (PET) to differentiate newly diagnosed glioblastomas from brain metastases. AMT-PET was performed in 36 adults with suspected brain malignancy. Tumoral AMT accumulation was measured by standardized uptake values (SUVs). Tracer kinetic analysis was also performed to separate tumoral net tryptophan transport (by AMT volume of distribution [VD]) from unidirectional uptake rates using dynamic PET and blood input function. Differentiating the accuracy of these PET variables was evaluated and compared to conventional MRI. For glioblastoma/metastasis differentiation, tumoral AMT SUV showed the highest accuracy (74%) and the tumor/cortex VD ratio had the highest positive predictive value (82%). The combined accuracy of MRI (size of contrast-enhancing lesion) and AMT-PET reached up to 93%. For ring-enhancing lesions, tumor/cortex SUV ratios were higher in glioblastomas than in metastatic tumors and could differentiate these two tumor types with > 90% accuracy. These results demonstrate that evaluation of tryptophan accumulation by PET can enhance pretreatment differentiation of glioblastomas and metastatic brain tumors. This approach may be particularly useful in patients with a newly diagnosed solitary ring-enhancing mass.
- Published
- 2013
23. Successful surgical treatment of an inflammatory lesion associated with new-onset refractory status epilepticus.
- Author
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Juhász C, Buth A, Chugani DC, Kupsky WJ, Chugani HT, Shah AK, and Mittal S
- Subjects
- Carbon Radioisotopes, Electroencephalography, Glial Fibrillary Acidic Protein metabolism, Humans, Interleukin-1beta metabolism, Magnetic Resonance Imaging, Male, Middle Aged, Positron-Emission Tomography, Temporal Lobe diagnostic imaging, Temporal Lobe surgery, Tryptophan analogs & derivatives, Encephalitis etiology, Encephalitis surgery, Neurosurgery methods, Status Epilepticus complications
- Abstract
New-onset refractory status epilepticus (NORSE) has high morbidity and mortality. The authors describe the successful surgical treatment of a 56-year-old man presenting with NORSE. Magnetic resonance imaging showed a left temporal lobe lesion suspicious for a low-grade tumor, while PET imaging with the alpha[(11)C]methyl-L-tryptophan (AMT) radiotracer showed increased cortical uptake extending beyond this lesion and partly overlapping with epileptogenic cortex mapped by chronic intracranial electroencephalographic monitoring. Resection of the epileptic focus resulted in long-term seizure freedom, and the nonresected portion of the PET-documented abnormality normalized. Histopathology showed reactive gliosis and inflammatory markers in the AMT-PET-positive cortex. Molecular imaging of neuroinflammation can be instrumental in the management of NORSE by guiding placement of intracranial electrodes or assessing the extent and severity of inflammation for antiinflammatory interventions.
- Published
- 2013
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24. Tryptophan PET in pretreatment delineation of newly-diagnosed gliomas: MRI and histopathologic correlates.
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Kamson DO, Juhász C, Buth A, Kupsky WJ, Barger GR, Chakraborty PK, Muzik O, and Mittal S
- Subjects
- Adult, Aged, Aged, 80 and over, Carbon Isotopes, Female, Gadolinium, Humans, Imaging, Three-Dimensional, Magnetic Resonance Imaging, Male, Middle Aged, Statistics, Nonparametric, Young Adult, Brain diagnostic imaging, Brain pathology, Brain Neoplasms diagnosis, Glioma diagnosis, Positron-Emission Tomography, Tryptophan
- Abstract
Pretreatment delineation of infiltrating glioma volume remains suboptimal with current neuroimaging techniques. Gadolinium-enhanced T1-weighted (T1-Gad) MR images often underestimate the true extent of the tumor, while T2-weighted images preferentially highlight peritumoral edema. Accumulation of α-[(11)C]methyl-L-tryptophan (AMT) on positron emission tomography (PET) has been shown in gliomas. To determine whether increased uptake on AMT-PET would detect tumor-infiltrated brain tissue outside the contrast-enhancing region and differentiate it from peritumoral vasogenic edema, volumes and spatial concordance of T1-Gad and T2 MRI abnormalities as well as AMT-PET abnormalities were analyzed in 28 patients with newly-diagnosed WHO grade II-IV gliomas. AMT-accumulating grade I meningiomas were used to define an AMT uptake cutoff threshold that detects the tumor but excludes peri-meningioma vasogenic edema. Tumor infiltration in AMT-accumulating areas was studied in stereotactically-resected specimens from patients with glioblastoma. In the 28 gliomas, mean AMT-PET-defined tumor volumes were greater than the contrast-enhancing volume, but smaller than T2 abnormalities. Volume of AMT-accumulating tissue outside MRI abnormalities increased with higher tumor proliferative index and was the largest in glioblastomas. Tumor infiltration was confirmed by histopathology from AMT-positive regions outside contrast-enhancing glioblastoma mass, while no or minimal tumor cells were found in AMT-negative specimens. These results demonstrate that increased AMT accumulation on PET detects glioma-infiltrated brain tissue extending beyond the contrast-enhanced tumor mass. While tryptophan uptake is low in peritumoral vasogenic edema, AMT-PET can detect tumor-infiltrated brain outside T2-lesions. Thus, AMT-PET may assist pretreatment delineation of tumor infiltration, particularly in high-grade gliomas.
- Published
- 2013
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25. Quantitative PET imaging of tryptophan accumulation in gliomas and remote cortex: correlation with tumor proliferative activity.
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Juhász C, Chugani DC, Barger GR, Kupsky WJ, Chakraborty PK, Muzik O, and Mittal S
- Subjects
- Adult, Aged, Biological Transport, Brain Neoplasms metabolism, Case-Control Studies, Cell Proliferation, Cerebral Cortex diagnostic imaging, Female, Glioma metabolism, Humans, Ki-67 Antigen metabolism, Kinetics, Male, Middle Aged, Tryptophan metabolism, Young Adult, Brain Neoplasms diagnostic imaging, Brain Neoplasms pathology, Cerebral Cortex metabolism, Glioma diagnostic imaging, Glioma pathology, Positron-Emission Tomography, Tryptophan analogs & derivatives
- Abstract
Purpose: PET studies with α[C-11]methyl-L-tryptophan (AMT) have shown decreased serotonin synthesis based on a decrease of the unidirectional uptake rate (K-complex) in neuropsychiatric conditions such as autism and depression. Increased AMT K-complex in tumors can indicate increased tryptophan metabolism via the immunosuppressive kynurenine pathway. Moreover, apparent AMT volume of distribution (VD') reflects net tryptophan transport from blood to tissue. We evaluated if kinetic parameters (K-complex, VD') of AMT, measured by PET, can predict the proliferative activity of glioma, and if these AMT parameters are altered in the remote cortex., Methods: We evaluated dynamic AMT PET images of 30 adult patients with grade 2 to 4 gliomas according to the World Health Organization's classification to determine tumoral AMT VD' and K-complex values, which were correlated with tumor proliferative activity as assessed by the Ki-67 labeling index in resected tumor specimens. We also compared cortical VD' and K-complex values between patients with glioma and healthy controls., Results: Both VD' and K-complex values were significantly higher in gliomas than in the contralateral cortex (VD', P < 0.001; K-complex, P < 0.001). Tumoral VD' values and tumor/cortex VD' ratios, but not the K-complex, showed strong positive correlations with the proliferative activity of glioma (P ≤ 0.001). The contralateral frontal cortex showed decreased AMT VD' and K-complex in patients with glioma compared with those in controls (P ≤ 0.01)., Conclusions: Increased net amino acid transport into tumor tissue, quantified by PET, can serve as an imaging marker of the proliferative activity of glioma. The data also suggest a glioma-induced down-regulation of cortical serotonin synthesis, likely mediated by shunting of tryptophan from serotonin synthesis to kynurenine metabolism.
- Published
- 2012
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26. Fetal central nervous system injury in third trimester stillbirth: a clinicopathologic study of 63 cases.
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Jacques SM, Kupsky WJ, Giorgadze T, and Qureshi F
- Subjects
- Female, Fetus, Gestational Age, Humans, Placenta pathology, Pregnancy, Pregnancy Complications pathology, Pregnancy Trimester, Third, Brain Injuries pathology, Fetal Diseases pathology, Stillbirth
- Abstract
We report the neuropathologic findings and clinicopathologic associations in 63 3rd trimester singleton stillborn fetuses. All were ≥ 28 weeks estimated gestational age (EGA) with complete autopsies, including placental examination. Fetuses with chromosomal abnormalities, major congenital anomalies, and intrapartum demise were excluded. The cases were divided into those with abruption (n = 12) and those with unexplained fetal demise (n = 51). The latter group was then subdivided by gestational age with 3 subgroups (preterm 28 to < 32 weeks EGA (n = 16), preterm 32 to <37 weeks EGA (n = 13), and term 37-41 weeks EGA (n = 22). Each group was further subdivided as appropriate-for-gestational age/large-for-gestational age (AGA/LGA) or small-for-gestational age (SGA). Placental lesions were also evaluated and correlated with brain lesions. Established or recent injury involving gray or white matter was seen in 88% of the fetuses with unexplained demise versus 42% with abruption (P = 0.001). The most common form of brain injury was established gray matter damage, seen in 65% of the fetuses with unexplained demise versus 25% with abruption (P = 0.021), the most common pattern being established pontosubicular neuronal necrosis plus established neuronal necrosis in other sites. There was no significant difference in the frequency of brain injury between the SGA fetuses and AGA/LGA fetuses or between the unexplained stillbirth preterm and term subgroups, and there was no unequivocal correlation between placental lesions and brain lesions. Brain injury, most frequently established gray matter damage, is seen in the majority of stillborn infants with unexplained demise, indicating that the brain injury predates the period immediately before death.
- Published
- 2012
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27. Accurate differentiation of recurrent gliomas from radiation injury by kinetic analysis of α-11C-methyl-L-tryptophan PET.
- Author
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Alkonyi B, Barger GR, Mittal S, Muzik O, Chugani DC, Bahl G, Robinette NL, Kupsky WJ, Chakraborty PK, and Juhász C
- Subjects
- Adult, Aged, Brain Neoplasms metabolism, Brain Neoplasms pathology, Diagnosis, Differential, Female, Fluorodeoxyglucose F18, Glioma metabolism, Glioma pathology, Humans, Image Processing, Computer-Assisted, Ki-67 Antigen metabolism, Male, Middle Aged, Neoplasm Recurrence, Local diagnostic imaging, ROC Curve, Radiation Injuries metabolism, Radiation Injuries pathology, Radionuclide Imaging, Reference Standards, Reproducibility of Results, Tryptophan pharmacokinetics, Brain Neoplasms diagnostic imaging, Glioma diagnostic imaging, Radiation Injuries diagnostic imaging, Radiopharmaceuticals pharmacokinetics, Tryptophan analogs & derivatives
- Abstract
Unlabelled: PET of amino acid transport and metabolism may be more accurate than conventional neuroimaging in differentiating recurrent gliomas from radiation-induced tissue changes. α-(11)C-methyl-l-tryptophan ((11)C-AMT) is an amino acid PET tracer that is not incorporated into proteins but accumulates in gliomas, mainly because of tumoral transport and metabolism via the immunomodulatory kynurenine pathway. The aim of this study was to evaluate the usefulness of (11)C-AMT PET supplemented by tracer kinetic analysis for distinguishing recurrent gliomas from radiation injury., Methods: Twenty-two (11)C-AMT PET scans were obtained in adult patients who presented with a lesion suggestive of tumor recurrence on conventional MRI 1-6 y (mean, 3 y) after resection and postsurgical radiation of a World Health Organization grade II-IV glioma. Lesional standardized uptake values were calculated, as well as lesion-to-contralateral cortex ratios and 2 kinetic (11)C-AMT PET parameters (volume of distribution [VD], characterizing tracer transport, and unidirectional uptake rate [K]). Tumor was differentiated from radiation-injured tissue by histopathology (n = 13) or 1-y clinical and MRI follow-up (n = 9). Accuracy of tumor detection by PET variables was assessed by receiver-operating-characteristic analysis., Results: All (11)C-AMT PET parameters were higher in tumors (n = 12) than in radiation injury (n = 10) (P ≤ 0.012 in all comparisons). The lesion-to-cortex K-ratio most accurately identified tumor recurrence, with highly significant differences both in the whole group (P < 0.0001) and in lesions with histologic verification (P = 0.006); the area under the receiver-operating-characteristic curve was 0.99. A lesion-to-cortex K-ratio threshold of 1.39 (i.e., a 39% increase) correctly differentiated tumors from radiation injury in all but 1 case (100% sensitivity and 91% specificity). In tumors that were high-grade initially (n = 15), a higher lesion-to-cortex K-ratio threshold completely separated recurrent tumors (all K-ratios ≥ 1.70) from radiation injury (all K-ratios < 1.50) (100% sensitivity and specificity)., Conclusion: Kinetic analysis of dynamic (11)C-AMT PET images may accurately differentiate between recurrent World Health Organization grade II-IV infiltrating gliomas and radiation injury. Separation of unidirectional uptake rates from transport can enhance the differentiating accuracy of (11)C-AMT PET. Applying the same approach to other amino acid PET tracers might also improve their ability to differentiate recurrent gliomas from radiation injury.
- Published
- 2012
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28. Increased L-[1-11 C] leucine uptake in the leptomeningeal angioma of sturge-weber syndrome: a PET study.
- Author
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Alkonyi B, Chugani HT, Muzik O, Chugani DC, Sundaram SK, Kupsky WJ, Batista CE, and Juhász C
- Subjects
- Adolescent, Brain metabolism, Child, Child, Preschool, Female, Hemangioma metabolism, Humans, Infant, Leucine metabolism, Male, Meningeal Neoplasms metabolism, Positron-Emission Tomography, Radiopharmaceuticals metabolism, Sturge-Weber Syndrome metabolism, Brain diagnostic imaging, Hemangioma diagnostic imaging, Meningeal Neoplasms diagnostic imaging, Sturge-Weber Syndrome diagnostic imaging
- Abstract
Background and Purpose: We used L-[1-(11) C]leucine (LEU) positron emission tomography (PET) to measure amino acid uptake in children with Sturge-Weber syndrome (SWS), and to relate amino acid uptake measures with glucose metabolism., Methods: LEU and 2-deoxy-2[(18) F]fluoro-D-glucose (FDG) PET were performed in 7 children (age: 5 months-13 years) with unilateral SWS. Asymmetries of LEU uptake in the posterior brain region, underlying the angioma and in frontal cortex, were measured and correlated with glucose hypometabolism. Kinetic analysis of LEU uptake was performed in 4 patients., Results: Increased LEU standard uptake value (SUV, mean: 15.1%) was found in the angioma region in 6 patients, and smaller increases in LEU SUV (11.5%) were seen in frontal cortex in 4 of the 6 patients, despite normal glucose metabolism in frontal regions. High LEU SUV was due to both increased tracer transport (3/4 patients) and high protein synthesis rates (2/4). FDG SUV asymmetries in the angioma region were inversely related to LEU SUV asymmetries (r=-.83, P= .042)., Conclusions: Increased amino acid uptake in the angioma region and also in less affected frontal regions may provide a marker of pathological mechanisms contributing to chronic brain damage in children with SWS., (Copyright © 2011 by the American Society of Neuroimaging.)
- Published
- 2012
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29. Increased tryptophan transport in epileptogenic dysembryoplastic neuroepithelial tumors.
- Author
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Alkonyi B, Mittal S, Zitron I, Chugani DC, Kupsky WJ, Muzik O, Chugani HT, Sood S, and Juhász C
- Subjects
- Adolescent, Carbon Isotopes pharmacokinetics, Child, Child, Preschool, Electroencephalography, Epilepsy diagnostic imaging, Epilepsy metabolism, Epilepsy surgery, Female, Fluorodeoxyglucose F18, Gene Expression Regulation, Neoplastic physiology, Humans, Indoleamine-Pyrrole 2,3,-Dioxygenase metabolism, Magnetic Resonance Imaging, Male, Neoplasms, Neuroepithelial diagnostic imaging, Positron-Emission Tomography, Teratoma diagnostic imaging, Tryptophan analogs & derivatives, Tryptophan pharmacokinetics, Epilepsy etiology, Large Neutral Amino Acid-Transporter 1 metabolism, Neoplasms, Neuroepithelial complications, Neoplasms, Neuroepithelial metabolism, Teratoma complications, Teratoma metabolism
- Abstract
Dysembryoplastic neuroepithelial tumors (DNTs) are typically hypometabolic but can show increased amino acid uptake on positron emission tomography (PET). To better understand mechanisms of amino acid accumulation in epileptogenic DNTs, we combined quantitative α-[(11)C]methyl-L: -tryptophan (AMT) PET with tumor immunohistochemistry. Standardized uptake values (SUVs) of AMT and glucose were measured in 11 children with temporal lobe DNT. Additional quantification for AMT transport and metabolism was performed in 9 DNTs. Tumor specimens were immunostained for the L: -type amino acid transporter 1 (LAT1) and indoleamine 2,3-dioxygenase (IDO), a key enzyme of the immunomodulatory kynurenine pathway. All 11 tumors showed glucose hypometabolism, while mean AMT SUVs were higher than normal cortex in eight DNTs. Further quantification showed increased AMT transport in seven and high AMT metabolic rates in three DNTs. Two patients showing extratumoral cortical increases of AMT SUV had persistent seizures despite complete tumor resection. Resected DNTs showed moderate to strong LAT1 and mild to moderate IDO immunoreactivity, with the strongest expression in tumor vessels. These results indicate that accumulation of tryptophan in DNTs is driven by high amino acid transport, mediated by LAT1, which can provide the substrate for tumoral tryptophan metabolism through the kynurenine pathway, that can produce epileptogenic metabolites. Increased AMT uptake can extend to extratumoral cortex, and presence of such cortical regions may increase the likelihood of recurrent seizures following surgical excision of DNTs.
- Published
- 2012
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30. Complete recovery of acute monocular visual loss following endoscopic resection of anterior clinoid mucocele: case report and review of the literature.
- Author
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Nundkumar N, Mittal M, Kupsky WJ, Folbe A, and Mittal S
- Subjects
- Acute Disease, Adult, Endoscopy methods, Humans, Male, Mucocele complications, Vision, Monocular physiology, Mucocele surgery, Nerve Compression Syndromes etiology, Optic Nerve Diseases etiology, Recovery of Function physiology, Vision Disorders etiology
- Abstract
Mucoceles are chronic non-neoplastic cystic lesions lined by mucus-secreting respiratory epithelium in the paranasal sinuses. Mucocele of the anterior clinoid process is a particularly rare entity most often presenting with rapidly progressive monocular blindness. The authors describe the case of a 32 year-old man who presented with acute painless visual loss in the left eye. Workup revealed an expansile lesion of the left anterior clinoid process with associated optic nerve compression. The patient underwent emergent endoscopic-assisted transnasal decompression of the optic nerve with full recovery of visual function. Early diagnosis and prompt surgical intervention optimizes the chances of functional regain of visual acuity., (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Published
- 2012
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31. Primary intracranial plasma cell granulomas presenting as malignant neoplasms.
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Puntambekar P, Santhakumar S, Kupsky WJ, Tselis A, and Mittal S
- Subjects
- Adult, Brain Diseases surgery, Brain Neoplasms pathology, Diagnosis, Differential, Female, Granuloma, Plasma Cell surgery, Humans, Immunohistochemistry, Male, Brain Diseases pathology, Granuloma, Plasma Cell pathology
- Abstract
Plasma cell granuloma (PCG) is an uncommon non-neoplastic mass lesion of unknown etiology. It is characterized by a polyclonal proliferation of chronic inflammatory cells, mostly mature plasma and other mononuclear cells. PCGs arising in the central nervous system are particularly rare. We report two additional cases of intracranial PCG exclusively involving the brain parenchyma. A 47 year-old woman, presenting with partial motor seizures and fluent aphasia, underwent complete excision of a well-demarcated, enhancing left parietal mass. The second patient was a 56 year-old man presenting with headaches and right-sided weakness who underwent stereotactic biopsy of an ill-defined, heterogeneously enhancing lesion in the left basal ganglia. Immunohistochemical analysis of surgical specimens showed polyclonal plasma cells and mature lymphocytes but no etiological agent. A histopathologic diagnosis of intracranial PCG was made in both cases. PCG should be part of the differential diagnosis of enhancing mass lesions of the brain. The etiology and natural history of these tumor-like lesions is not fully understood. Complete surgical excision appears to be curative. Lesions where total resection is not possible may benefit from adjuvant treatment including corticosteroids and possibly radiation therapy.
- Published
- 2012
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32. Isolated intracranial Whipple's disease--report of a rare case and review of the literature.
- Author
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Mohamed W, Neil E, Kupsky WJ, Juhász C, Mittal S, and Santhakumar S
- Subjects
- Anti-Bacterial Agents therapeutic use, Brain Diseases cerebrospinal fluid, Brain Diseases drug therapy, Cognition Disorders cerebrospinal fluid, Cognition Disorders drug therapy, Female, Humans, Middle Aged, Whipple Disease cerebrospinal fluid, Whipple Disease drug therapy, Brain Diseases diagnosis, Cognition Disorders diagnosis, Whipple Disease diagnosis
- Abstract
Introduction: Whipple's disease (WD) is a rare multisystemic infectious disease that can involve a variety of organs namely the gastrointestinal tract, lymphatic system, heart and nervous system. Myorhythmia is a hallmark of WD. Isolated CNS involvement is very rare., Case: We present a 50 year-old African-American woman with rapid cognitive decline, visual hallucinations, insomnia, dysarthria, and gait unsteadiness. She subsequently developed pendular nystagmus and gaze paresis. Serial brain MRI scans showed T2 hyperintense lesions in the left striatum and right parahippocampal gyrus. FDG-PET scan showed marked increase of glucose uptake in the left putamen. Serum and CSF PCR for Tropheryma whipplei was negative. Stereotactic biopsy of the lesion and tissue PCR was consistent with WD., Review of Literature: A systematic review identified 24 cases of isolated intracranial presentation of WD since 1975. Cases with systemic and extracranial manifestations were excluded., Discussion: In patients with rapidly progressive cognitive decline with negative workup for common etiologies, there should be a high index of suspicion for WD. Diagnosis of WD remains a challenge as traditional methods commonly fail to culture T. whipplei. PET scans can help in identifying areas of inflammation that can be biopsied. Our case proves that a negative serum and CSF PCR should not exclude CNS WD and a brain biopsy of the lesion with PCR assay should be performed when possible., (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Published
- 2011
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33. Distinct pathogenic processes between Fig4-deficient motor and sensory neurons.
- Author
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Katona I, Zhang X, Bai Y, Shy ME, Guo J, Yan Q, Hatfield J, Kupsky WJ, and Li J
- Subjects
- Animals, Autophagy, Cells, Cultured, Charcot-Marie-Tooth Disease genetics, Charcot-Marie-Tooth Disease pathology, Disease Models, Animal, Flavoproteins genetics, Ganglia, Spinal pathology, Ganglia, Spinal ultrastructure, Humans, Intracellular Signaling Peptides and Proteins, Lysosomal-Associated Membrane Protein 2 metabolism, Mice, Mice, Knockout, Mitochondria metabolism, Motor Neurons cytology, Nerve Degeneration metabolism, Nerve Degeneration pathology, Niemann-Pick C1 Protein, Phosphoinositide Phosphatases, Proteins metabolism, Receptor, IGF Type 2 metabolism, Sensory Receptor Cells cytology, Vacuoles metabolism, Vacuoles ultrastructure, Flavoproteins metabolism, Motor Neurons pathology, Motor Neurons physiology, Sensory Receptor Cells pathology, Sensory Receptor Cells physiology
- Abstract
Loss of function of the FIG4 gene causes Charcot-Marie-Tooth disease (CMT)-4J with many features also found in motor neuron disease (MND). Mechanisms for the degeneration are unknown. We investigated this using Fig4-deficient pale tremor (plt) mice, a mouse model of CMT4J. Ultrastructural studies in sensory neurons of dorsal root ganglion (DRG) confirmed abundant vacuoles with membrane disruption. The vacuoles became detectable as early as postnatal day 4 in the DRG. However, the vacuoles were absent or minimal in the spinal motor neurons or cortical neurons in 2- to 5-week-old plt mice. Instead, a large number of electron-dense organelles, reminiscent of those in lysosomal storage disorders, accumulated in the motor neurons, but not in the sensory neurons of DRG. This accumulation was associated with increased levels of lysosomal proteins, such as LAMP2 and NPC1, but not mannose-6-phosphate receptor, an endosomal protein that is usually excluded from the lysosomes. Our results suggest that Fig4 deficiency affects motor neurons differently from sensory neurons by mechanisms involving excessive retention of molecules in lysosomes or disruption of vacuolated organelles. These two distinct pathological changes may contribute to neuronal degeneration., (© 2011 The Authors. European Journal of Neuroscience © 2011 Federation of European Neuroscience Societies and Blackwell Publishing Ltd.)
- Published
- 2011
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34. Non-Langerhans cell histiocytosis with isolated CNS involvement: an unusual variant of Erdheim-Chester disease.
- Author
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Conley A, Manjila S, Guan H, Guthikonda M, Kupsky WJ, and Mittal S
- Subjects
- Diagnosis, Differential, Fatal Outcome, Female, Histiocytosis, Non-Langerhans-Cell pathology, Histiocytosis, Sinus pathology, Humans, Immunohistochemistry, Magnetic Resonance Imaging, Microscopy, Electron, Transmission, Middle Aged, Xanthomatosis pathology, Brain Diseases pathology, Erdheim-Chester Disease pathology
- Abstract
Benign histiocytic proliferations are identified by their component cells and classified as either Langerhans cell histiocytosis or non-Langerhans cell histiocytosis. We report a 58-year-old Caucasian woman who presented with diabetes insipidus and was found to harbor a large suprasellar mass. Histopathological analysis was consistent with non-LCH. The differential diagnoses included juvenile xanthogranuloma, adult-onset xanthogranuloma, xanthoma disseminatum, Rosai-Dorfman disease, and Erdheim-Chester disease. Immunohistochemical examination demonstrated a proliferation of large lipid-laden histiocytic cells which were positive for CD68, negative for S100 protein, and showed only faint, background staining for CD1a. We present a case of an autopsy-confirmed non-Langerhans cell histiocytosis limited to the central nervous system and evaluated with both immunohistochemical and ultrastructural studies. Based on the multifocality, anatomic distribution, and immunostaining features, a diagnosis of Erdheim-Chester disease was made. This is only the second reported case of Erdheim-Chester disease with intracranial involvement but absence of extracerebral manifestations. Given the overlapping clinicopathologic, radiographic, and immunohistochemical profiles, differentiating between these rare histiocytic disorders can often present a significant diagnostic challenge. A systematic approach using all available clinical, laboratory, radiographic, histologic, immunohistochemical and ultrastructural data is essential for proper discrimination between the numerous histiocytoses., (© 2010 Japanese Society of Neuropathology.)
- Published
- 2010
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35. Leptomeningeal dissemination of a pediatric neoplasm with 1p19q deletion showing mixed immunohistochemical features of an oligodendroglioma and neurocytoma.
- Author
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Rhiew RB, Manjila S, Lozen A, Guthikonda M, Sood S, and Kupsky WJ
- Subjects
- Brain Neoplasms chemistry, Brain Neoplasms genetics, Child, DNA Mutational Analysis methods, Diagnosis, Differential, Humans, Male, Meningeal Carcinomatosis chemistry, Meningeal Carcinomatosis physiopathology, Neurocytoma chemistry, Neurocytoma genetics, Oligodendroglioma chemistry, Oligodendroglioma genetics, Treatment Outcome, Brain Neoplasms complications, Chromosomes, Human, Pair 1 genetics, Gene Deletion, Meningeal Carcinomatosis etiology, Mutation genetics, Neurocytoma complications, Oligodendroglioma complications
- Abstract
Leptomeningeal dissemination of an oligodendroglioma is rarely reported in the neurosurgical literature, especially in cases with a classical 1p19q deletion. The authors describe a case wherein a 1p19q deletion in a disseminated tumor with mixed immunohistochemical features of oligodendroglioma and neurocytoma was encountered and treated. Stereotactic right frontal craniotomy was undertaken for obtaining definitive histological diagnosis. The results revealed a neuroectodermal neoplasm with histologic and immunohistochemical features of oligodendroglioma and neurocytoma. FISH analysis confirmed classical 1p19q deletion. The patient was treated postoperatively with chemotherapy and radiation therapy. He showed good clinical response and remains alive 16 months after diagnosis.
- Published
- 2010
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36. Synchronous meningioma and anaplastic large cell lymphoma.
- Author
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Colen CB, Rayes M, Kupsky WJ, and Guthikonda M
- Subjects
- Aged, Craniotomy, Fibrosis, Humans, Lymphoma, Large-Cell, Anaplastic complications, Lymphoma, Large-Cell, Anaplastic surgery, Male, Meningeal Neoplasms complications, Meningeal Neoplasms surgery, Meningioma complications, Meningioma surgery, Lymphoma, Large-Cell, Anaplastic pathology, Meningeal Neoplasms pathology, Meningioma pathology
- Abstract
Synchronous primary brain tumors are exceedingly rare. When they occur, most cases are associated with metastatic disease. To the best of our knowledge, we report the first case of an atypical meningioma infiltrated by a T-cell-primary central nervous system lymphoma (PCNSL), specifically anaplastic large cell lymphoma (ALCL). We present a novel, unifying, plausible mechanism for its origin based on theories in the current literature. A 65-year-old man with a history of near-total resection of atypical meningioma presented with a complaint of progressive headaches. Imaging revealed recurrent tumor. Left frontal-temporal craniotomy with near-total tumor resection followed by radiation was performed. Recurrent symptomatic tumor led to repeat left frontotemporal craniotomy with tumor resection and partial anterior temporal lobectomy. Part of the specimen showed predominantly fibrotic neoplasm composed of nests and whorls of meningothelial cells, highlighted by epithelial membrane antigen (EMA) staining. The remainder of the specimen consisted of densely cellular neoplasm centered in connective tissue, including areas involved by meningioma. This tumor was composed of moderately large lymphoid cells with large nuclei, prominent nucleoli, and amphophilic cytoplasm. These cells were strongly immunoreactive for CD3 and CD30 but remained unstained with EMA, anaplastic lymphoma kinase-1 (ALK-1), CD15 or cytotoxic associated antigen TIA-1. Smaller mature lymphocytes, chiefly T-cells, were intermixed. The morphologic and immunohistochemical features were considered typical of anaplastic large T-cell lymphoma. The pathogenesis of this association may have been due to radiation-mediated breakdown of the blood-brain barrier with subsequent T-cell infiltration and proliferation. We advocate aggressive resection and long-term surveillance for individuals with metastasis, especially higher-grade neoplasms that receive radiotherapy.
- Published
- 2010
- Full Text
- View/download PDF
37. Onset of vulvodynia in a woman ultimately diagnosed with Creutzfeldt-Jakob disease.
- Author
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Reichman O, Tselis A, Kupsky WJ, and Sobel JD
- Subjects
- Creutzfeldt-Jakob Syndrome pathology, Female, Humans, Magnetic Resonance Imaging, Middle Aged, Creutzfeldt-Jakob Syndrome complications, Creutzfeldt-Jakob Syndrome diagnosis, Vulvodynia etiology
- Abstract
Background: Vulvodynia, defined as vulvar pain or burning in the presence of normal vulvar appearance, is common and is associated with chronic pain syndromes and psychiatric disorders., Case: A postmenopausal woman complained of vulvar burning. Causes for vulvar burning including yeast infection, estrogen deficiency, and contact dermatitis were excluded. Vulvovaginal examination was normal. Subsequently, she complained of headaches, insomnia, and depression. She developed ataxic gait with rapidly progressive dementia. Brain biopsy confirmed the diagnosis of Creutzfeldt-Jakob disease, and 3 weeks later she lapsed into coma and died., Conclusion: This report is unique in that a rare disease, known to result in neuronal damage, mimicked symptoms of vulvodynia in its initial phase. This supports the hypothesis that vulvodynia is a neuropathic syndrome originating in the nervous system.
- Published
- 2010
- Full Text
- View/download PDF
38. Most primary central nervous system diffuse large B-cell lymphomas occurring in immunocompetent individuals belong to the nongerminal center subtype: a retrospective analysis of 31 cases.
- Author
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Hattab EM, Martin SE, Al-Khatib SM, Kupsky WJ, Vance GH, Stohler RA, Czader M, and Al-Abbadi MA
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Biomarkers, Tumor analysis, Central Nervous System Neoplasms genetics, Central Nervous System Neoplasms metabolism, Gene Rearrangement, Genes, Immunoglobulin Heavy Chain, Genes, bcl-2, Genes, myc, Germinal Center pathology, Humans, Immunohistochemistry, In Situ Hybridization, Fluorescence, Kaplan-Meier Estimate, Ki-67 Antigen metabolism, Lymphoma, Large B-Cell, Diffuse genetics, Lymphoma, Large B-Cell, Diffuse metabolism, Middle Aged, Retrospective Studies, Young Adult, Central Nervous System Neoplasms pathology, Lymphoma, Large B-Cell, Diffuse pathology
- Abstract
Primary central nervous system lymphomas are rare neoplasms characterized by a dismal prognosis relative to other extranodal lymphomas. Approximately 98% of primary central nervous system lymphomas are of B-cell origin, and most belong to the diffuse large B-cell type. Recently, diffuse large B-cell lymphomas have been subcategorized into germinal center and nongerminal center types based on gene expression profiles and immunohistochemical expression of CD10, Bcl-6, and MUM1. Studies have shown that the overall survival rate of the germinal center group is better than that of the nongerminal center lymphomas. In this study, 31 cases of primary central nervous system lymphomas of the diffuse large B-cell type were retrieved, reviewed, and immunostained for CD10, Bcl-6, MUM1, and Ki-67. Subclassification was carried out as described earlier, where CD10 and/or Bcl-6 positivity and negativity for MUM1 were considered characteristic of germinal center subtype and the opposite expression of nongerminal center subtype. Furthermore, the proliferative activity was semiquantitatively assessed using percent positive cells staining with Ki-67. Of the 31 cases examined, 26 (84%) were found to belong to the nongerminal center type. The Ki-67 index in these 26 cases ranged from 30 to 90% (mean, 69%). Five cases were categorized as the germinal center subtype. They had an Ki-67 index between 70 and 90% (mean, 78%). Interestingly, none of our patients were known to be HIV positive. One patient had a 10-year history of orthotopic liver transplant. We also performed fluorescence in situ hybridization analysis on formalin-fixed material and found that 38% of the cases where tissue was available had abnormalities of MYC/IGH and/or IGH/BCL2. We conclude that most primary central nervous system diffuse large B-cell lymphomas are of the nongerminal center origin. Regardless of the germinal center status, all cases showed a high proliferative rate. A statistically significant difference in the overall survival between the two groups was not seen.
- Published
- 2010
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- View/download PDF
39. Imaging correlates of differential expression of indoleamine 2,3-dioxygenase in human brain tumors.
- Author
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Batista CE, Juhász C, Muzik O, Kupsky WJ, Barger G, Chugani HT, Mittal S, Sood S, Chakraborty PK, and Chugani DC
- Subjects
- Adolescent, Adult, Aged, Brain Neoplasms genetics, Child, Child, Preschool, Female, Glioma diagnostic imaging, Glioma genetics, Humans, Immunohistochemistry, Indoleamine-Pyrrole 2,3,-Dioxygenase genetics, Male, Middle Aged, Positron-Emission Tomography methods, Tryptophan analogs & derivatives, Tryptophan metabolism, Young Adult, Brain Neoplasms diagnostic imaging, Diagnostic Imaging methods, Indoleamine-Pyrrole 2,3,-Dioxygenase metabolism
- Abstract
Background: Tryptophan catabolism via the kynurenine pathway, mediated by indoleamine 2,3-dioxygenase (IDO), is a mechanism involved in tumor immunoresistance. Positron emission tomography (PET) with alpha-[(11)C]methyl-L-tryptophan (AMT) can quantify transport and metabolism of tryptophan in infiltrating gliomas and glioneuronal tumors. In the present study, we investigated whether increased tryptophan metabolism in brain tumors measured by PET is related to expression of IDO in resected brain tumor specimens., Methods: IDO expression was assessed by immunohistochemistry in tumor specimens from 15 patients (median age, 34 years) with primary brain tumors who underwent AMT PET scanning before tumor resection. Patterns of IDO expression were compared between low- and high-grade tumors and also to AMT transport and metabolism measured on PET., Results: IDO immunoreactivity was seen in tumor cells in six of seven low-grade tumors but only in one of eight high-grade tumors (p = 0.01); three of these latter tumors showed endothelial staining only. Low-grade neoplasms showed lower transport rate (p < 0.01) but higher metabolic rate (p = 0.003) for AMT as compared to high-grade tumors. AMT metabolic rates were lower in tumor samples with no or minimal IDO expression as compared to those with widespread IDO staining (p = 0.017)., Conclusion: Low-grade tumors show widespread IDO expression, while IDO expression in high-grade brain tumors can be absent or largely confined to endothelial cells. AMT PET can be useful to identify brain tumors with different profiles of IDO expression, thus providing a useful imaging marker for emerging treatments targeting tumor IDO activity.
- Published
- 2009
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- View/download PDF
40. Sudden death, febrile seizures, and hippocampal and temporal lobe maldevelopment in toddlers: a new entity.
- Author
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Kinney HC, Chadwick AE, Crandall LA, Grafe M, Armstrong DL, Kupsky WJ, Trachtenberg FL, and Krous HF
- Subjects
- Child, Preschool, Female, Humans, Infant, Male, Prone Position, Sleep, Death, Sudden etiology, Hippocampus abnormalities, Seizures, Febrile etiology, Temporal Lobe abnormalities
- Abstract
Recently, we reported hippocampal and temporal lobe abnormalities in 5 toddlers with sudden unexplained death in childhood (SUDC). The association of these anomalies with a high incidence (40%) of individual/family histories of simple febrile seizures in the cases raised concern that febrile seizures can be associated with death. In a series of 64 toddlers with sudden death, we tested the hypothesis that an SUDC subset is characterized by hippocampal and temporal lobe maldevelopment and an individual and/or family history of simple familial seizures. Cases of sudden and unexplained death in children aged 1.0 to 5.9 years (median 1.7 years) were divided into groups based upon a history of febrile or nonfebrile seizures, familial febrile seizures, and autopsy classification of cause of death. Forty-nine of the 64 cases (77%) were classified as SUDC, of which 40% had an individual/family history of febrile seizures. Of the 26 SUDC cases with available hippocampal sections, 62% (16/26) had hippocampal and temporal lobe anomalies, including 82% (9/11) of cases with an individual/family history of febrile seizures. Cases with these anomalies were all found dead during a sleep period, typically in the prone (87%) position. We conclude that a potential new entity may account for the majority of SUDC in toddlers, defined by sleep-related death in the prone position, individual/family history of febrile seizures, and hippocampal and temporal lobe anomalies. The mechanism of death appears analogous to sudden death in (temporal lobe) epilepsy, with a putative unwitnessed seizure during sleep leading to airway occlusion and death. This study mandates further research into the potential link between simple febrile seizures and death.
- Published
- 2009
- Full Text
- View/download PDF
41. Neuronal loss in Pelizaeus-Merzbacher disease differs in various mutations of the proteolipid protein 1.
- Author
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Sima AA, Pierson CR, Woltjer RL, Hobson GM, Golden JA, Kupsky WJ, Schauer GM, Bird TD, Skoff RP, and Garbern JY
- Subjects
- Adult, Age Factors, Chromosomes, Human, X, Genetic Markers, Humans, Immunohistochemistry, Male, Middle Aged, Mutation, Myelin Sheath genetics, Myelin Sheath pathology, Neuroglia pathology, Brain pathology, Cell Death genetics, Myelin Proteolipid Protein genetics, Neurons pathology, Pelizaeus-Merzbacher Disease genetics, Pelizaeus-Merzbacher Disease pathology
- Abstract
Mutations affecting proteolipid protein 1 (PLP1), the major protein in central nervous system myelin, cause the X-linked leukodystrophy Pelizaeus-Merzbacher disease (PMD). We describe the neuropathologic findings in a series of eight male PMD subjects with confirmed PLP1 mutations, including duplications, complete gene deletion, missense and exon-skipping. While PLP1 mutations have effects on oligodendrocytes that result in mutation-specific degrees of dysmyelination, our findings indicate that there are also unexpected effects in the central nervous system resulting in neuronal loss. Although length-dependent axonal degeneration has been described in PLP1 null mutations, there have been no reports on neuronal degeneration in PMD patients. We now demonstrate widespread neuronal loss in PMD. The patterns of neuronal loss appear to be dependent on the mutation type, suggesting selective vulnerability of neuronal populations that depends on the nature of the PLP1 disturbance. Nigral neurons, which were not affected in patients with either null or severe misfolding mutations, and thalamic neurons appear particularly vulnerable in PLP1 duplication and deletion patients, while hippocampal neuronal loss was prominent in a patient with complete PLP1 gene deletion. All subjects showed cerebellar neuronal loss. The patterns of neuronal involvement may explain some clinical findings, such as ataxia, being more prominent in PMD than in other leukodystrophies. While the precise pathogenetic mechanisms are not known, these observations suggest that defective glial functions contribute to neuronal pathology.
- Published
- 2009
- Full Text
- View/download PDF
42. Middle cerebral artery pseudoaneurysm formation following stereotactic biopsy.
- Author
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Rayes M, Bahgat DA, Kupsky WJ, and Mittal S
- Subjects
- Adult, Aneurysm, False surgery, Antineoplastic Agents therapeutic use, Biopsy, Needle adverse effects, Brain Neoplasms pathology, Humans, Intracranial Aneurysm surgery, Lymphoma pathology, Magnetic Resonance Angiography, Magnetic Resonance Imaging, Male, Middle Cerebral Artery injuries, Middle Cerebral Artery pathology, Middle Cerebral Artery surgery, Neurosurgical Procedures instrumentation, Neurosurgical Procedures methods, Postoperative Complications physiopathology, Stereotaxic Techniques adverse effects, Surgical Instruments, Treatment Outcome, Vascular Surgical Procedures instrumentation, Vascular Surgical Procedures methods, Aneurysm, False etiology, Aneurysm, False pathology, Intracranial Aneurysm etiology, Intracranial Aneurysm pathology, Postoperative Complications etiology, Postoperative Complications pathology
- Published
- 2008
- Full Text
- View/download PDF
43. Alpha-methyl-l-tryptophan positron emission tomography in epilepsy with cortical developmental malformations.
- Author
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Wakamoto H, Chugani DC, Juhász C, Muzik O, Kupsky WJ, and Chugani HT
- Subjects
- Adolescent, Brain diagnostic imaging, Carbon Radioisotopes, Child, Child, Preschool, Electroencephalography, Epilepsies, Partial diagnostic imaging, Epilepsies, Partial etiology, Epilepsy etiology, Female, Fluorodeoxyglucose F18, Humans, Injections, Intravenous, Magnetic Resonance Imaging, Male, Malformations of Cortical Development complications, Reproducibility of Results, Tryptophan administration & dosage, Tryptophan analogs & derivatives, Cerebral Cortex diagnostic imaging, Epilepsy diagnostic imaging, Malformations of Cortical Development diagnostic imaging, Positron-Emission Tomography methods
- Abstract
Preliminary studies suggest that alpha[(11)C]methyl-l-tryptophan positron emission tomography can detect the epileptic focus within malformations of cortical development. We determined the sensitivity and specificity of alpha-[(11)C]methyl-l-tryptophan positron emission tomography in identifying epileptic focus in children with intractable, neocortical epilepsy with and without malformations of cortical development. Seventy-three epileptic children were classified into lesional and nonlesional groups, and compared regarding focal increased alpha-[(11)C]methyl-l-tryptophan uptake. The sensitivity and specificity of focal increased alpha-[(11)C]methyl-l-tryptophan uptake, using intracranial electroencephalogram localization of seizure onset as the standard, were compared between lesional and nonlesional groups. The specificity of alpha-[(11)C]methyl-l-tryptophan positron emission tomography for detecting seizure onset lobe was equally high in lesional (97%) and nonlesional groups (100%), whereas sensitivity was higher in the lesional than the nonlesional group (47% versus 29%; P = 0.047). The incidence of alpha-[(11)C]methyl-l-tryptophan uptake abnormality was higher in the lesional than the nonlesional group (P < 0.01). Alpha-[(11)C]methyl-l-tryptophan positron emission tomography localized and visualized epileptogenic regions in 25% of patients with nonlocalizing magnetic resonance imaging. Although overall sensitivity of alpha-[(11)C]methyl-l-tryptophan positron emission tomography in identifying neocortical epileptic focus is modest, specificity is extremely high. When an alpha-[(11)C]methyl-l-tryptophan focus is detected, it likely represents the epileptogenic region to be resected.
- Published
- 2008
- Full Text
- View/download PDF
44. Glioblastoma multiforme after microsurgery for acoustic neuroma without radiotherapy: limitations of the Cahan criteria.
- Author
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Hoa M, Rhiew R, Kupsky WJ, Guthikonda M, and Monsell EM
- Subjects
- Brain Neoplasms pathology, Brain Neoplasms surgery, Female, Glioblastoma pathology, Glioblastoma surgery, Humans, Magnetic Resonance Imaging, Microsurgery, Middle Aged, Brain Neoplasms etiology, Glioblastoma etiology, Neuroma, Acoustic surgery
- Published
- 2008
- Full Text
- View/download PDF
45. Sudden death in toddlers associated with developmental abnormalities of the hippocampus: a report of five cases.
- Author
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Kinney HC, Armstrong DL, Chadwick AE, Crandall LA, Hilbert C, Belliveau RA, Kupsky WJ, and Krous HF
- Subjects
- Autopsy, Case-Control Studies, Child, Preschool, Female, Humans, Infant, Male, Seizures, Febrile physiopathology, Death, Sudden etiology, Hippocampus abnormalities, Hippocampus pathology
- Abstract
Sudden unexplained death in childhood (SUDC) is the sudden death of a child older than 1 year of age that remains unexplained after review of the clinical history, circumstances of death, and autopsy with appropriate ancillary testing. We report here 5 cases of SUDC in toddlers that we believe define a new entity associated with hippocampal anomalies at autopsy. All of the toddlers died unexpectedly during the night, apparently during sleep. Within 48 hours before death, 2 toddlers had fever, 3 had a minor upper respiratory tract infection, and 3 experienced minor head trauma. There was a history of febrile seizures in 2 (40%) and a family history of febrile seizures in 2 (40%). Hippocampal findings included external asymmetry and 2 or more microdysgenetic features. The incidence of certain microdysgenetic features was substantially increased in the temporal lobes of these 5 cases compared with the temporal lobes of 39 (control) toddlers with the causes of death established at autopsy (P < 0.01). We propose that these 5 cases define a potential subset of SUDC whose sudden death is caused by an unwitnessed seizure arising during sleep in the anomalous hippocampus and producing cardiopulmonary arrest. Precipitating factors may be fever, infection, and/or minor head trauma. Suggested risk factors are a history of febrile seizures and/or a family history of febrile seizures. Future studies are needed to confirm these initial findings and to define the putative links between sudden death, hippocampal anomalies, and febrile seizures in toddlers.
- Published
- 2007
- Full Text
- View/download PDF
46. Gliosarcoma with multiple extracranial metastases: case report and review of the literature.
- Author
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Beaumont TL, Kupsky WJ, Barger GR, and Sloan AE
- Subjects
- Brain Neoplasms diagnosis, Brain Neoplasms therapy, Chemotherapy, Adjuvant, Craniotomy, Fatal Outcome, Glioblastoma drug therapy, Glioblastoma radiotherapy, Glioblastoma surgery, Gliosarcoma diagnosis, Gliosarcoma pathology, Humans, Magnetic Resonance Imaging, Male, Middle Aged, Neoplasm Recurrence, Local, Radiotherapy, Adjuvant, Stereotaxic Techniques, Brain Neoplasms pathology, Gliosarcoma secondary, Liver Neoplasms secondary, Neoplasms, Second Primary diagnosis, Neoplasms, Second Primary pathology, Splenic Neoplasms secondary, Temporal Lobe, Thoracic Neoplasms secondary
- Abstract
Gliosarcoma is a rare malignant neoplasm of the central nervous system with a propensity for metastasis. There are fewer than 20 reported cases of extracranial metastases of gliosarcoma with the majority of cases reflecting a tendency for hematogenous dissemination. Here we describe the case of a 47-year-old man who developed pervasive extracranial metastases from a temporal gliosarcoma following radio- and chemotherapy for a primary glioblastoma. The patient initially presented with progressively worsening headaches, left-sided weakness and numbness associated with right temporo-parietal mass for which he underwent craniotomy with stereotactic gross-total excision. Two months postoperatively, interstitial brachytherapy and external beam radiotherapy were initiated. The patient initially declined chemotherapy. The tumor recurred twice and the patient underwent re-operation and multiple courses of chemotherapy; histopathological diagnosis remained glioblastoma multiforme. Nineteen months following initial resection the patient's clinical status deteriorated and CT scan demonstrated multiple intrathoracic, hepatic and splenic lesions. Postmortem examination revealed widespread, infiltrating gliosarcoma with intravascular gliomatosis and extensive visceral metastases. This is the first report of pervasive extracranial metastases to numerous sites, several of which have not been previously reported. The histogenesis and the potential role of therapeutic irradiation in the development of gliosarcoma are briefly reviewed.
- Published
- 2007
- Full Text
- View/download PDF
47. Major myelin protein gene (P0) mutation causes a novel form of axonal degeneration.
- Author
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Li J, Bai Y, Ianakova E, Grandis M, Uchwat F, Trostinskaia A, Krajewski KM, Garbern J, Kupsky WJ, and Shy ME
- Subjects
- Adult, Aged, Animals, Autopsy, Biomarkers metabolism, Charcot-Marie-Tooth Disease genetics, Charcot-Marie-Tooth Disease pathology, Charcot-Marie-Tooth Disease physiopathology, Female, Humans, Myelin P0 Protein metabolism, Myelin Sheath metabolism, Myelin Sheath pathology, Myelin Sheath ultrastructure, Nerve Fibers, Myelinated metabolism, Nerve Fibers, Myelinated pathology, Nerve Fibers, Myelinated ultrastructure, Peripheral Nerves metabolism, Peripheral Nerves pathology, Peripheral Nerves ultrastructure, Retrograde Degeneration metabolism, Retrograde Degeneration pathology, Mutation, Myelin P0 Protein genetics, Retrograde Degeneration genetics
- Abstract
Mutations in the major peripheral nervous system (PNS) myelin protein, myelin protein zero (MPZ), cause Charcot-Marie-Tooth Disease type 1B (CMT1B), typically thought of as a demyelinating peripheral neuropathy. Certain MPZ mutations, however, cause adult onset neuropathy with minimal demyelination but pronounced axonal degeneration. Mechanism(s) for this phenotype are unknown. We performed an autopsy of a 73-year-old woman with a late-onset neuropathy caused by an H10P MPZ mutation whose nerve conduction studies suggested severe axonal loss but no demyelination. The autopsy demonstrated axonal loss and reorganization of the molecular architecture of the axolemma. Segmental demyelination was negligible. In addition, we identified focal nerve enlargements containing MPZ and ubiquitin either in the inner myelin intralaminar and/or periaxonal space that separates axons from myelinating Schwann cells. Taken together, these data confirmed that a mutation in MPZ can cause axonal neuropathy, in the absence of segmental demyelination, thus uncoupling the two pathological processes. More important, it also provided potential molecular mechanisms as to how the axonal degeneration occurred: either by disruption of glial-axon interaction by protein aggregates or by alterations in the molecular architecture of internodes and paranodes. This report represents the first study in which the molecular basis of axonal degeneration in the late-onset CMT1B has been explored in human tissue., (2006 Wiley-Liss, Inc.)
- Published
- 2006
- Full Text
- View/download PDF
48. Elephant brain. Part I: gross morphology, functions, comparative anatomy, and evolution.
- Author
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Shoshani J, Kupsky WJ, and Marchant GH
- Subjects
- Animals, Brain blood supply, Cats, Chinchilla, Equidae, Female, Guinea Pigs, Haplorhini, Humans, Hyraxes, Male, Pan troglodytes, Sheep, Wolves, Biological Evolution, Brain anatomy & histology, Brain physiology, Elephants anatomy & histology, Elephants physiology
- Abstract
We report morphological data on brains of four African, Loxodonta africana, and three Asian elephants, Elephas maximus, and compare findings to literature. Brains exhibit a gyral pattern more complex and with more numerous gyri than in primates, humans included, and in carnivores, but less complex than in cetaceans. Cerebral frontal, parietal, temporal, limbic, and insular lobes are well developed, whereas the occipital lobe is relatively small. The insula is not as opercularized as in man. The temporal lobe is disproportionately large and expands laterally. Humans and elephants have three parallel temporal gyri: superior, middle, and inferior. Hippocampal sizes in elephants and humans are comparable, but proportionally smaller in elephant. A possible carotid rete was observed at the base of the brain. Brain size appears to be related to body size, ecology, sociality, and longevity. Elephant adult brain averages 4783 g, the largest among living and extinct terrestrial mammals; elephant neonate brain averages 50% of its adult brain weight (25% in humans). Cerebellar weight averages 18.6% of brain (1.8 times larger than in humans). During evolution, encephalization quotient has increased by 10-fold (0.2 for extinct Moeritherium, approximately 2.0 for extant elephants). We present 20 figures of the elephant brain, 16 of which contain new material. Similarities between human and elephant brains could be due to convergent evolution; both display mosaic characters and are highly derived mammals. Humans and elephants use and make tools and show a range of complex learning skills and behaviors. In elephants, the large amount of cerebral cortex, especially in the temporal lobe, and the well-developed olfactory system, structures associated with complex learning and behavioral functions in humans, may provide the substrate for such complex skills and behavior.
- Published
- 2006
- Full Text
- View/download PDF
49. In vivo uptake and metabolism of alpha-[11C]methyl-L-tryptophan in human brain tumors.
- Author
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Juhász C, Chugani DC, Muzik O, Wu D, Sloan AE, Barger G, Watson C, Shah AK, Sood S, Ergun EL, Mangner TJ, Chakraborty PK, Kupsky WJ, and Chugani HT
- Subjects
- Adolescent, Adult, Aged, Brain Neoplasms diagnosis, Brain Neoplasms diagnostic imaging, Carbon Radioisotopes, Cerebral Cortex chemistry, Cerebral Cortex diagnostic imaging, Child, Child, Preschool, Electroencephalography methods, Electroencephalography standards, Female, Gadolinium, Glucose metabolism, Humans, Infant, Magnetic Resonance Imaging methods, Magnetic Resonance Imaging standards, Male, Middle Aged, Neoplasm Staging, Positron-Emission Tomography methods, Positron-Emission Tomography standards, Seizures metabolism, Sensitivity and Specificity, Tryptophan metabolism, Tryptophan pharmacokinetics, Tryptophan standards, Brain Neoplasms metabolism, Cerebral Cortex metabolism, Tryptophan analogs & derivatives
- Abstract
Abnormal metabolism of tryptophan has been implicated in modulation of tumor cell proliferation and immunoresistance. alpha-[(11)C]Methyl-L-tryptophan (AMT) is a PET tracer to measure cerebral tryptophan metabolism in vivo. In the present study, we have measured tumor tryptophan uptake in 40 patients with primary brain tumors using AMT PET and standard uptake values (SUV). Tryptophan metabolism was further quantified in 23 patients using blood input data. Estimates of the volume of distribution (VD') and the metabolic rate constant (k(3)') were calculated and related to magnetic resonance imaging (MRI) and histology findings. All grade II to IV gliomas and glioneuronal tumors showed increased AMT SUV, including all recurrent/residual tumors. Gadolinium enhancement on MRI was associated with high VD' values, suggesting impaired blood-brain barrier, while k(3)' values were not related to contrast enhancement. Low-grade astrocytic gliomas showed increased tryptophan metabolism, as measured by k(3)'. In contrast, oligodendrogliomas showed high VD' values but lower k(3)' as compared with normal cortex. In astrocytic tumors, low grade was associated with high k(3)' and lower VD', while high-grade tumors showed the reverse pattern. The findings show high AMT uptake in primary and residual/recurrent gliomas and glioneuronal tumors. Increased AMT uptake can be due to increased metabolism of tryptophan and/or high volume of distribution, depending on tumor type and grade. High tryptophan metabolic rates in low-grade tumors may indicate activation of the kynurenine pathway, a mechanism regulating tumor cell growth. AMT PET might be a useful molecular imaging method to guide therapeutic approaches aimed at controlling tumor cell proliferation by acting on tryptophan metabolism.
- Published
- 2006
- Full Text
- View/download PDF
50. Recovery and prognosticators of paralysis in West Nile virus infection.
- Author
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Cao NJ, Ranganathan C, Kupsky WJ, and Li J
- Subjects
- Adult, Aged, Aged, 80 and over, Antigens, CD metabolism, Electromyography methods, Extremities physiopathology, Female, Functional Laterality, Humans, Immunohistochemistry methods, Male, Microscopy, Electron methods, Middle Aged, Motor Neurons physiology, Muscle Weakness etiology, Muscle Weakness metabolism, Muscle Weakness pathology, Muscle, Skeletal metabolism, Muscle, Skeletal pathology, Muscle, Skeletal ultrastructure, Neural Conduction, Paralysis pathology, Prognosis, West Nile Fever pathology, Paralysis etiology, West Nile Fever complications
- Abstract
Previous studies have demonstrated that lesions of the anterior horn motor neurons are the primary pathology in patients with paralysis due to West Nile virus (WNV) infection. To characterize recovery and identify prognostic factors for the recovery of paralysis, we investigated 11 patients with electrophysiology testing and muscle biopsy, and one with autopsy. We found that limb weakness was markedly asymmetric and differed between upper and lower extremities, suggesting focal or segmental involvement of the spinal cord anterior horn. This was supported by segmental depletion of spinal motor neurons at autopsy. Clinical recovery was variable during a 21-month follow-up period. To explain variability, we performed motor unit number estimation (MUNE) in six patients. MUNE values and strength were correlated in tested muscles. We also detected motor nerve terminal damages in muscle biopsies, suggesting another possible mechanism for transient weakness and variable recovery. We conclude that the type of pathological lesions may vary in paralytic WNV infection, and different degrees or combinations of motor neuron loss and motor nerve terminal changes may account for the observed degrees of weakness and recovery.
- Published
- 2005
- Full Text
- View/download PDF
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