23 results on '"Kuntz-Simon, G."'
Search Results
2. Validation of two commercial real-time RT-PCR kits for rapid and specific diagnosis of classical swine fever virus
- Author
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Le Dimna, M., Vrancken, R., Koenen, F., Bougeard, S., Mesplède, A., Hutet, E., Kuntz-Simon, G., and Le Potier, M.F.
- Published
- 2008
- Full Text
- View/download PDF
3. Longitudinal study of respiratory infection patterns of breeding sows in 5 farrow-to-finish herds
- Author
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Fablet, C., Marois, C., Kuntz-Simon, G., Rose, N., Dorenlor, V., Eono, F., Eveno, E., Jolly, J.P., Le Devendec, L., Tocqueville, V., Quéguiner, S., Gorin, S., Kobisch, M., Madec, F., and Agence Française de Sécurité Sanitaire des Aliments (AFSSA)
- Subjects
infection dynamics ,respiratory diseases ,animal diseases ,sows ,pathogen detection - Abstract
International audience; A longitudinal study was carried out in five French farrow-to-finish herds differently affected by respiratory diseases to describe the carrying and infection patterns of batches of sows to various respiratory pathogens during gestation and lactation. An entire batch of sows was followed during two successive reproduction cycles. Nasal, tonsillar and oro-pharyngeal swabs and blood samples were taken from each sow nine and four weeks before farrowing and one and four weeks after farrowing. , , , and were detected from swab samples using PCR assays. Blood samples were tested for antibodies against , serotypes 1-9-11 and 2, Porcine Circovirus type-2 (PCV-2) and Porcine Reproductive and Respiratory Syndrome virus (PRRSV) by ELISA tests. Antibodies against HN, HN and HN swine influenza viruses (SIV) of European lineages were tested by hemagglutination inhibition assay. The results indicated that is widespread among sows (67.1% of PCR-positive sows). , , were detected by PCR in 30.9%, 24.6% and 23.4% of the sows respectively. Antibodies against were recovered from more than 55% of the sows in all herds whereas the micro-organism was detected in 2.4% of the sows. Although PCV-2 and SIV infections were highly prevalent, the PRRSV infection patterns ranged from no infection in farms mildly affected by respiratory diseases to active circulation in more severely affected herds. The sow population thus constitutes a reservoir for a continuous circulation of respiratory pathogens and needs to be properly considered in control strategies.
- Published
- 2010
4. Letter: Influenza A (H1N1) infection in pigs
- Author
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Brookes, S.M., Irvine, R.M., Nunez, A., Clifford, D., van Essen, S., Brown, I.H., Reeth, K., Kuntz-Simon, G., Loeffen, W.L.A., Foni, E., Larsen, L., Matrosovich, M., Bublot, M., Maldonado, J., Beer, M., and Cattoli, G.
- Subjects
CVI - Divisie Virologie ,Life Science ,CVI - Division Virology - Published
- 2009
5. Replication, Pathogenesis and Transmission of Pandemic (H1N1) 2009 Virus in Non-Immune Pigs
- Author
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Brookes, S.M., Nunez, A., Choudhury, B., Matrosovich, M., Essen, S.C., Clifford, D., Slomka, M.J., Kuntz-Simon, G., Garcon, F., Nash, B., Hanna, A., Heegaard, P.M.H., Queguiner, S., Chiapponi, C., Bublot, M., Garcia, J.M., Gardner, R., Foni, E., Loeffen, W.L.A., Larsen, L., Reeth, K., Banks, J., Irvine, R.M., Brown, I.H., Brookes, S.M., Nunez, A., Choudhury, B., Matrosovich, M., Essen, S.C., Clifford, D., Slomka, M.J., Kuntz-Simon, G., Garcon, F., Nash, B., Hanna, A., Heegaard, P.M.H., Queguiner, S., Chiapponi, C., Bublot, M., Garcia, J.M., Gardner, R., Foni, E., Loeffen, W.L.A., Larsen, L., Reeth, K., Banks, J., Irvine, R.M., and Brown, I.H.
- Abstract
The declaration of the human influenza A pandemic (H1N1) 2009 (H1N1/09) raised important questions, including origin and host range [1,2]. Two of the three pandemics in the last century resulted in the spread of virus to pigs (H1N1, 1918; H3N2, 1968) with subsequent independent establishment and evolution within swine worldwide [3]. A key public and veterinary health consideration in the context of the evolving pandemic is whether the H1N1/09 virus could become established in pig populations [4]. We performed an infection and transmission study in pigs with A/California/07/09. In combination, clinical, pathological, modified influenza A matrix gene real time RT-PCR and viral genomic analyses have shown that infection results in the induction of clinical signs, viral pathogenesis restricted to the respiratory tract, infection dynamics consistent with endemic strains of influenza A in pigs, virus transmissibility between pigs and virus-host adaptation events. Our results demonstrate that extant H1N1/09 is fully capable of becoming established in global pig populations. We also show the roles of viral receptor specificity in both transmission and tissue tropism. Remarkably, following direct inoculation of pigs with virus quasispecies differing by amino acid substitutions in the haemagglutinin receptor-binding site, only virus with aspartic acid at position 225 (225D) was detected in nasal secretions of contact infected pigs. In contrast, in lower respiratory tract samples from directly inoculated pigs, with clearly demonstrable pulmonary pathology, there was apparent selection of a virus variant with glycine (225G). These findings provide potential clues to the existence and biological significance of viral receptor-binding variants with 225D and 225G during the 1918 pandemic [5].
- Published
- 2010
6. Virological Surveillance and Preliminary Antigenic Characterization of Influenza Viruses in Pigs in Five European Countries from 2006 to 2008
- Author
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Kyriakis, C. S., primary, Brown, I. H., additional, Foni, E., additional, Kuntz‐Simon, G., additional, Maldonado, J., additional, Madec, F., additional, Essen, S. C., additional, Chiapponi, C., additional, and Van Reeth, K., additional
- Published
- 2011
- Full Text
- View/download PDF
7. Genetic and Antigenic Evolution of Swine Influenza Viruses in Europe and Evaluation of Their Zoonotic Potential
- Author
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Kuntz-Simon, G., primary and Madec, F., additional
- Published
- 2009
- Full Text
- View/download PDF
8. Two outbreaks of classical swine fever in wild boar in France
- Author
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Pol, F., primary, Rossi, S., additional, Mesplède, A., additional, Kuntz-Simon, G., additional, and Le Potier, M-F., additional
- Published
- 2008
- Full Text
- View/download PDF
9. Sodium Valproate, an Anticonvulsant Drug, Stimulates Human Immunodeficiency Virus Type 1 Replication Independently of Glutathione Levels
- Author
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Moog, C., primary, Kuntz-Simon, G., additional, Caussin-Schwemling, C., additional, and Obert, G., additional
- Published
- 1996
- Full Text
- View/download PDF
10. Sodium valproate, an anticonvulsant drug, stimulates human cytomegalovirus replication
- Author
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Kuntz-Simon, G., primary and Obert, G., additional
- Published
- 1995
- Full Text
- View/download PDF
11. Initiation of DNA replication at palindromic telomeres is mediated by a duplex-to-hairpin transition induced by the minute virus of mice nonstructural protein NS1.
- Author
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Willwand, K, Mumtsidu, E, Kuntz-Simon, G, and Rommelaere, J
- Abstract
The linear single-stranded DNA genome of the minute virus of mice (MVM) is replicated via a double-stranded replicative form (RF) intermediate. Amplification of this RF is initiated by the folding-back of palindromic sequences serving as primers for strand-displacement synthesis and formation of dimeric RF DNA. Using an in vitro replication assay and a cloned MVM DNA template, we observed hairpin-primed DNA replication at both MVM DNA termini, with a bias toward right-end initiation. Initiation of DNA replication is favored by nuclear components of A9 cell extract and highly stimulated by the MVM nonstructural protein NS1. Hairpin-primed DNA replication is also observed in the presence of NS1 and the Klenow fragment of the Escherichia coli DNA polymerase I. Addition of ATPgammaS (adenosine 5'-O-(thiotriphosphate)) blocks the initiation of DNA replication but not the extension of pre-existing hairpin primers formed in the presence of NS1 only. The NS1-mediated unwinding of the right-end palindrome may account for the recently reported capacity of NS1 for driving dimer RF synthesis in vitro.
- Published
- 1998
12. Temperature sensitivity on growth and/or replication of H1N1, H1N2 and H3N2 influenza A viruses isolated from pigs and birds in mammalian cells.
- Author
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Massin P, Kuntz-Simon G, Barbezange C, Deblanc C, Oger A, Marquet-Blouin E, Bougeard S, van der Werf S, and Jestin V
- Subjects
- Animals, Birds, Cell Line, Chickens, Dogs, Humans, Influenza A Virus, H1N1 Subtype growth & development, Influenza A Virus, H1N1 Subtype isolation & purification, Influenza A Virus, H1N2 Subtype growth & development, Influenza A Virus, H1N2 Subtype isolation & purification, Influenza A Virus, H3N2 Subtype growth & development, Influenza A Virus, H3N2 Subtype isolation & purification, Influenza in Birds virology, Influenza, Human virology, Orthomyxoviridae Infections virology, Phylogeny, Swine, Swine Diseases virology, Viral Load, Influenza A Virus, H1N1 Subtype physiology, Influenza A Virus, H1N2 Subtype physiology, Influenza A Virus, H3N2 Subtype physiology, Orthomyxoviridae Infections veterinary, Temperature, Virus Replication physiology
- Abstract
Influenza A viruses have been isolated from a wide range of animal species, aquatic birds being the reservoir for their genetic diversity. Avian influenza viruses can be transmitted to humans, directly or indirectly through an intermediate host like pig. This study aimed to define in vitro conditions that could prove useful to evaluate the potential of influenza viruses to adapt to a different host. Growth of H1N1, H1N2 and H3N2 influenza viruses belonging to different lineages isolated from birds or pigs prior to 2005 was tested on MDCK or NPTr cell lines in the presence or absence of exogenous trypsin. Virus multiplication was compared at 33, 37 and 40 degrees C, the infection site temperatures in human, swine and avian hosts, respectively. Temperature sensitivity of PB2-, NP- and M-RNA replication was also tested by quantitative real-time PCR. Multiplication of avian viruses was cold-sensitive, whatever cell type. By contrast, temperature sensitivity of swine viruses was found to depend on the virus and the host cell: for an H1N1 swine isolate from 1982, multiplication was cold-sensitive on NPTr cells and undetectable at 40 degrees C. From genetic analyses, it appears that temperature sensitivity could involve other residues than PB2 residue 627 and could affect other steps of the replication cycle than replication., (Copyright 2009 Elsevier B.V. All rights reserved.)
- Published
- 2010
- Full Text
- View/download PDF
13. Replication, pathogenesis and transmission of pandemic (H1N1) 2009 virus in non-immune pigs.
- Author
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Brookes SM, Núñez A, Choudhury B, Matrosovich M, Essen SC, Clifford D, Slomka MJ, Kuntz-Simon G, Garcon F, Nash B, Hanna A, Heegaard PM, Quéguiner S, Chiapponi C, Bublot M, Garcia JM, Gardner R, Foni E, Loeffen W, Larsen L, Van Reeth K, Banks J, Irvine RM, and Brown IH
- Subjects
- Animals, Antigens, Viral analysis, Antigens, Viral immunology, Base Sequence, Chick Embryo, Disease Outbreaks, Hemagglutinins, Viral chemistry, Hemagglutinins, Viral genetics, Humans, Immunohistochemistry, Influenza A Virus, H1N1 Subtype genetics, Influenza A Virus, H1N1 Subtype immunology, Influenza, Human epidemiology, Influenza, Human virology, Mutation, Orthomyxoviridae Infections pathology, Orthomyxoviridae Infections transmission, Respiratory System metabolism, Respiratory System pathology, Respiratory System virology, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA, Swine, Swine Diseases pathology, Viral Matrix Proteins genetics, Influenza A Virus, H1N1 Subtype pathogenicity, Orthomyxoviridae Infections veterinary, Swine Diseases virology, Virus Replication
- Abstract
The declaration of the human influenza A pandemic (H1N1) 2009 (H1N1/09) raised important questions, including origin and host range [1], [2]. Two of the three pandemics in the last century resulted in the spread of virus to pigs (H1N1, 1918; H3N2, 1968) with subsequent independent establishment and evolution within swine worldwide [3]. A key public and veterinary health consideration in the context of the evolving pandemic is whether the H1N1/09 virus could become established in pig populations [4]. We performed an infection and transmission study in pigs with A/California/07/09. In combination, clinical, pathological, modified influenza A matrix gene real time RT-PCR and viral genomic analyses have shown that infection results in the induction of clinical signs, viral pathogenesis restricted to the respiratory tract, infection dynamics consistent with endemic strains of influenza A in pigs, virus transmissibility between pigs and virus-host adaptation events. Our results demonstrate that extant H1N1/09 is fully capable of becoming established in global pig populations. We also show the roles of viral receptor specificity in both transmission and tissue tropism. Remarkably, following direct inoculation of pigs with virus quasispecies differing by amino acid substitutions in the haemagglutinin receptor-binding site, only virus with aspartic acid at position 225 (225D) was detected in nasal secretions of contact infected pigs. In contrast, in lower respiratory tract samples from directly inoculated pigs, with clearly demonstrable pulmonary pathology, there was apparent selection of a virus variant with glycine (225G). These findings provide potential clues to the existence and biological significance of viral receptor-binding variants with 225D and 225G during the 1918 pandemic [5].
- Published
- 2010
- Full Text
- View/download PDF
14. Maternal stress during late gestation has moderate but long-lasting effects on the immune system of the piglets.
- Author
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Couret D, Jamin A, Kuntz-Simon G, Prunier A, and Merlot E
- Subjects
- Animals, Colostrum immunology, Female, Hydrocortisone analysis, Hydrocortisone blood, Immunoglobulin G analysis, Immunoglobulin G blood, Lymphocyte Activation, Organ Size, Pregnancy, Saliva chemistry, T-Lymphocyte Subsets, Tumor Necrosis Factor-alpha biosynthesis, Pregnancy Complications immunology, Prenatal Exposure Delayed Effects, Stress, Psychological immunology, Swine immunology
- Abstract
Events acting prenatally on developing foetuses are important determinants for disorders later in life. Prenatal stress (PNS) is one of these events. The purpose of this study was to determine the consequences of a repeated social stress applied during late gestation of the pregnant gilt on the immune system and hypothalamo-pituitary-adrenal (HPA) axis activity of the piglets from birth to two months of age. Pregnant gilts were submitted to repeated social stress which was induced by housing unfamiliar gilts in pairs modified twice a week during 4 weeks between days 77 and 105 of gestation (S group, n=18). Control gilts were housed in stable pairs during the same period (C group, n=18). Blood cortisol, haptoglobin and IgG levels, immune cell counts, mitogen-induced whole-blood proliferation and TNF-alpha production were evaluated in piglets at 4 days of age (D4), before and after weaning (D26 and 28) and before and after relocation to a new building (D60 and 62). We found that PNS did not affect growth rate of the progeny. It decreased the relative weight of adrenal glands on D4 (P<0.05) but plasma cortisol levels were similar in both groups at all ages. IgG levels in colostrum and in the serum of piglets were not affected. PNS decreased the total numbers of white blood cells, lymphocytes and granulocytes from D26 to D60 (P<0.05), the CD4(+)/CD8(+) T cell ratio on D4 (P<0.05), and LPS induced-TNF-alpha production on D60 (P<0.05). PNS increased the ConA-induced lymphocyte proliferation on D4 and D60 and the PWM-induced proliferation on D60 (P<0.05). Our results demonstrate that a repeated social stress applied to pregnant sows during late gestation can induce long-lasting effects on several parameters of the immune function of the offspring. These effects are not due to modifications of the HPA axis activity and may impair the abilities of the piglets to efficiently react against infections during the suckling period and around weaning.
- Published
- 2009
- Full Text
- View/download PDF
15. Influenza A (H1N1) infection in pigs.
- Author
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Brookes SM, Irvine RM, Nunez A, Clifford D, Essen S, Brown IH, Van Reeth K, Kuntz-Simon G, Loeffen W, Foni E, Larsen L, Matrosovich M, Bublot M, Maldonado J, Beer M, and Cattoli G
- Subjects
- Animals, Humans, Orthomyxoviridae Infections transmission, Orthomyxoviridae Infections virology, Swine, Swine Diseases transmission, Influenza A Virus, H1N1 Subtype isolation & purification, Orthomyxoviridae Infections veterinary, Swine Diseases virology
- Published
- 2009
- Full Text
- View/download PDF
16. [Emergence of a highly pathogenic variant of porcine reproductive and respiratory syndrome virus in Asia].
- Author
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Kuntz-Simon G and Vannier P
- Published
- 2008
- Full Text
- View/download PDF
17. Classical swine fever virus induces activation of plasmacytoid and conventional dendritic cells in tonsil, blood, and spleen of infected pigs.
- Author
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Jamin A, Gorin S, Cariolet R, Le Potier MF, and Kuntz-Simon G
- Subjects
- Animals, Antibody Formation, Antigens, CD immunology, Cells, Cultured, Cytokines immunology, Dendritic Cells cytology, Female, Gene Expression Regulation, Viral, Immunity, Cellular, Interleukins immunology, Male, Organ Specificity, Palatine Tonsil virology, Specific Pathogen-Free Organisms, Spleen virology, Swine, Viremia veterinary, Classical Swine Fever immunology, Classical Swine Fever Virus immunology, Cytokines biosynthesis, Dendritic Cells immunology, Interleukins biosynthesis
- Abstract
Classical swine fever virus (CSFV) compromises the host immune system, causing indirect leucopoenia and disruption of in vitro T cell stimulation capacity. In order to explore the potential role of dendritic cells (DC) in such phenomena, the activation of conventional DC (cDC) and plasmacytoid DC (pDC) in blood and secondary lymphoid organs of infected pigs was investigated in the early time course post-inoculation (pi), together with viral components dissemination and cytokine production in serum. Whereas CD11R1+CD172a+ cDC frequencies were markedly reduced in blood and spleen, analysis of CD4+CD172a+ pDC numbers revealed a rapid turn-over of this DC subset in tissues pi. Both subsets matured and were activated after infection, as demonstrated by down-regulation of CD1a, up-regulation of the co-stimulation molecule CD80/86 and expression of cytokines. cDC essentially expressed tumor necrosis factor alpha (TNF-alpha) and interleukin (IL)-10, whereas pDC produced alpha interferon (IFN-alpha) and IL-12. IFN-alpha and TNF-alpha productions revealed an enhancement of innate anti-viral immune responses. Detection of antigen activated B lymphocytes in tonsil T-cell areas at 72 h pi, subsequently to the transient translocation of the viral E2 protein within germinal centres at 48 h pi, indicates the initiation of humoral response. This response was also evidenced by an important IL-10 production in serum one week pi. IL-12 expression in organs, as well as transient detection of IL-18 and IFN-gamma in serum, reflected the initiation of cellular immune responses. However, the uncommonly high levels of TNF-alpha and IFN-alpha produced by DC and measured in serum early post-infection, together with IL-10 expression in spleen, could play a role in the disruption of immune system cells, either inducing apoptosis or impairing DC functionalities themselves.
- Published
- 2008
- Full Text
- View/download PDF
18. Characterization of conventional and plasmacytoid dendritic cells in swine secondary lymphoid organs and blood.
- Author
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Jamin A, Gorin S, Le Potier MF, and Kuntz-Simon G
- Subjects
- Animals, Antigens, CD immunology, Cytokines blood, Cytokines immunology, Cytokines metabolism, Dendritic Cells cytology, Dendritic Cells metabolism, Enzyme-Linked Immunosorbent Assay veterinary, Female, Flow Cytometry, Immunohistochemistry veterinary, Immunophenotyping veterinary, Interferon-beta biosynthesis, Interferon-beta immunology, Lymph Nodes cytology, Lymph Nodes immunology, Male, Microscopy, Fluorescence veterinary, Palatine Tonsil cytology, Palatine Tonsil immunology, Specific Pathogen-Free Organisms, Spleen cytology, Spleen immunology, Swine blood, T-Lymphocytes immunology, Dendritic Cells immunology, Lymphoid Tissue immunology, Swine immunology
- Abstract
Dendritic cells (DCs) act as antigen presenting cells that bridge innate and adaptive immune systems with the unique capacity to initiate primary T-cell responses and efficiently stimulate memory responses. In pig, little information is available about these cells in secondary lymphoid organs, the place where T cell activation usually occurs. As increased knowledge on DC is a necessary prerequisite to further understand their role in response to microbial infection or in protection after vaccination, we investigated the DC types that would be present in tonsil, spleen and non-subcutaneous lymph nodes in the steady state. One population was composed of CD172a(+)CD11R1(+)CD1(+/-)CD80/86(+/-) cells and would correspond to conventional DCs (cDC), while the other one was composed of CD172a(+)CD4(+)CD1(+/-)CD80/86(+/-) cells and would correspond to plasmacytoid DCs (pDC). These subsets were also detected in blood but spleen was the tissue with the higher frequency of such DCs. In lymphoid organs, most of cDC and pDC were in an immature status, as revealed by the low percentage of cells expressing the co-stimulatory molecule CD80/86. However, expression of that marker by 5% of DCs in organs and up to 15% in blood, together with lower expression of CD1a and expression of CD208, would indicate a partial activation and/or semi-maturation. Interestingly, 8% of tonsil pDC and 15% of blood pDC were shown to secrete IFN-alpha, while 18-20% of cDC expressed TNF-alpha in these tissues. Both cell types also expressed IL-12 and IL-10 in the steady state. Measurements of IFN-alpha, TNF-alpha, IL-12 and IL-10 levels in serum confirmed their production within immune homeostasis, whereas IL-6, IL-18 and IFN-gamma could not be detected. Altogether, these data complete knowledge on porcine immune system cells and will be a useful tool for further in vivo studies on porcine DC role in peripheral tolerance induction and in immune responses to pathogens.
- Published
- 2006
- Full Text
- View/download PDF
19. Validation of a real-time RT-PCR assay for rapid and specific diagnosis of Classical Swine Fever virus.
- Author
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Le Potier MF, Le Dimna M, Kuntz-Simon G, Bougeard S, and Mesplède A
- Subjects
- Animals, Classical Swine Fever epidemiology, Disease Outbreaks veterinary, Reagent Kits, Diagnostic, Reproducibility of Results, Reverse Transcriptase Polymerase Chain Reaction methods, Sensitivity and Specificity, Specific Pathogen-Free Organisms, Classical Swine Fever diagnosis, Classical Swine Fever virology, Classical Swine Fever Virus genetics, Classical Swine Fever Virus isolation & purification, Reverse Transcriptase Polymerase Chain Reaction veterinary, Swine virology
- Abstract
Two new real-time RT-PCR kits developed by LSI (TaqVet CSF) and ADIAGENE (Adiavet CSF) obtained a manufacturing agreement in France during the past year. For that purpose, the Classical Swine Fever (CSF) National Reference Laboratory (NRL) planned a schedule of conditions to be fulfilled by commercial real-time RT-PCR assays. The producers were asked to introduce an internal control to check the RNA extraction efficacy. The different criteria assessed were: sensitivity, specificity, especially "pestivirus specificity", reproducibility and easy handling, using 187 different samples distributed in four different panels. These samples were either CSFV inactivated strains or organs collected from CSF experimental SPF infected pigs, or naturally infected wild boars. All these samples were previously tested for genome detection using an RT-nested PCR assay and for virus isolation on cell culture. The LSI TaqVet kit was used for the CSF surveillance of wild boars in an area known to be infected, during the winter of 2004-2005. This field evaluation was carried out on 4710 spleen samples. In summary, the new CSF real-time RT-PCR assays have a higher predictive value than the current diagnostic standard, Virus Isolation.
- Published
- 2006
20. Baculovirus-expressed muscovy duck reovirus sigmaC protein induces serum neutralizing antibodies and protection against challenge.
- Author
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Kuntz-Simon G, Blanchard P, Cherbonnel M, Jestin A, and Jestin V
- Subjects
- Animals, Antibodies, Viral immunology, Antibody Specificity, Capsid Proteins genetics, Female, Fibroblasts virology, Male, Neutralization Tests, Orthoreovirus, Avian genetics, Pericarditis prevention & control, Pericarditis veterinary, Pericarditis virology, Poultry Diseases immunology, Reoviridae Infections immunology, Reoviridae Infections prevention & control, Specific Pathogen-Free Organisms, Tenosynovitis prevention & control, Tenosynovitis veterinary, Tenosynovitis virology, Vaccination veterinary, Vaccines, Synthetic immunology, Virus Cultivation, Antibodies, Viral biosynthesis, Capsid Proteins immunology, Ducks immunology, Genetic Vectors genetics, Nucleopolyhedroviruses genetics, Orthoreovirus, Avian immunology, Poultry Diseases prevention & control, RNA-Binding Proteins, Reoviridae Infections veterinary, Viral Vaccines immunology
- Abstract
Recombinant baculoviruses with sigmaC- or sigmaB-encoding gene from muscovy duck reovirus (DRV) were constructed. Western-blot analysis showed that sigmaC was more immunoreactive than sigmaB. Vaccination of SPF ducks with two injections, 3 weeks apart, of emulsions containing sigmaC or sigmaC + sigmaB elicited DRV-specific neutralizing antibodies. Following challenge, vaccination partially--or even totally in some cases--prevented the appearance of clinical symptoms. Moreover, immunization reduced the severity of reovirus-induced tenosynovitis and prevented pericarditis development during the course of the assay. Thus, DRV sigmaC, alone or co-expressed with sigmaB, appeared as a good candidate for vaccination of ducks (96/100 mots)., (Copyright 2002 Elsevier Science Ltd.)
- Published
- 2002
- Full Text
- View/download PDF
21. Muscovy duck reovirus sigmaC protein is atypically encoded by the smallest genome segment.
- Author
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Kuntz-Simon G, Le Gall-Reculé G, de Boisséson C, and Jestin V
- Subjects
- Amino Acid Sequence, Animals, Electrophoresis, Molecular Sequence Data, Orthoreovirus, Avian classification, Phylogeny, RNA, Viral analysis, Viral Proteins chemistry, Capsid Proteins, Ducks virology, Genome, Viral, Orthoreovirus, Avian genetics, Viral Proteins genetics
- Abstract
Although muscovy duck reovirus (DRV) shares properties with the reovirus isolated from chicken, commonly named avian reovirus (ARV), the two virus species are antigenically different. Similar to the DRV sigmaB-encoded gene (1201 bp long) previously identified, the three other double-stranded RNA small genome segments of DRV have been cloned and sequenced. They were 1325, 1191 and 1124 bp long, respectively, and contained conserved terminal sequences common to ARVs. They coded for single expression products, except the smallest (S4), which contained two overlapping open reading frames (ORF1 and ORF2). BLAST analyses revealed that the proteins encoded by the 1325 and 1191 bp genes shared high identity levels with ARV sigmaA and sigmaNS, respectively, and to a lesser extent with other orthoreovirus counterparts. No homology was found for the S4 ORF1-encoded p10 protein. The 29.4 kDa product encoded by S4 ORF2 appeared to be 25% identical to ARV S1 ORF3-encoded sigmaC, a cell-attachment oligomer inducing type-specific neutralizing antibodies. Introduction of large gaps in the N-terminal part of the DRV protein was necessary to improve DRV and ARV sigmaC amino acid sequence alignments. However, a leucine zipper motif was conserved and secondary structure analyses predicted a three-stranded alpha-helical coiled-coil feature at this amino portion. Thus, despite extensive sequence divergence, DRV sigmaC was suggested to be structurally and probably functionally related to ARV sigmaC. This work provides evidence for the diversity of the polycistronic S class genes of reoviruses isolated from birds and raises the question of the relative classification of DRV in the Orthoreovirus genus.
- Published
- 2002
- Full Text
- View/download PDF
22. Oncogenic transformation-dependent expression of a transcription factor NF-Y subunit.
- Author
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Gu Z, Kuntz-Simon G, Rommelaere J, and Cornelis J
- Subjects
- Animals, CCAAT-Enhancer-Binding Proteins, Cell Line, Transformed, Cells, Cultured, DNA metabolism, DNA-Binding Proteins chemistry, DNA-Binding Proteins genetics, Fibroblasts metabolism, Humans, Oncogene Protein p21(ras) physiology, Promoter Regions, Genetic, Protein Binding, Protein Isoforms chemistry, Protein Isoforms genetics, RNA Splicing, Rats, Rats, Inbred F344, Simian virus 40, Temperature, Cell Transformation, Neoplastic genetics, Cell Transformation, Viral genetics, DNA-Binding Proteins biosynthesis, Gene Expression Regulation, Neoplastic genetics, Protein Isoforms biosynthesis
- Abstract
As a result of differential splicing, one subunit of the nuclear factor Y (NF-Y) consists of two major isoforms designated short (NF-YaS) and long (NF-YaL). In proliferating normal human fibroblasts, NF-YaL is by far the more expressed isoform. Surprisingly, NF-YaS was found by immunoblotting to be as prominent as NF-YaL in simian virus 40 (SV40)-transformed cell derivatives. As a consequence, two NF-Y/DNA complexes, one containing the long and the other the short isoform, were formed with extracts from transformed cells and a target promoter element in electrophoretic mobility-shift assays. Only the complex containing NF-YaL was detected with extracts from normal fibroblasts. Furthermore, the NF-Y recognition motif contributed to promoter activation in SV40-transformed cells but not in normal, cells. Our finding links transcription stimulation in transformed cells to quantitative changes in the expression of an NF-Ya subunit.
- Published
- 1999
- Full Text
- View/download PDF
23. Neoplastic transformation-associated stimulation of the in vitro resolution of concatemer junction fragments from minute virus of mice DNA.
- Author
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Kuntz-Simon G, Bashir T, Rommelaere J, and Willwand K
- Subjects
- Cell Transformation, Neoplastic, Gene Amplification, Humans, DNA Replication, DNA, Viral chemistry, Minute Virus of Mice genetics, Virus Replication
- Abstract
Minute virus of mice (MVM) shows an oncotropic behavior reflected by its ability to amplify its genome more efficiently in a number of transformed versus normal cells. In vivo and in vitro studies revealed that the major effect of cell transformation on MVM DNA replication occurs at the level of double-stranded replicative-form amplification. In particular, resolution of MVM DNA concatemers into monomers was found to be highly sensitive to neoplastic transformation.
- Published
- 1999
- Full Text
- View/download PDF
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