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Your search keyword '"Kunimatsu, Ryo"' showing total 234 results
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234 results on '"Kunimatsu, Ryo"'

7. Surgical Orthodontic Treatment for Skeletal Maxillary Protrusion in Sturge-Weber Syndrome: A Case Report and Review of the Literature

Author

Kado, Isamu, primary, Ogashira, Shintaro, additional, Ono, Shigehiro, additional, Koizumi, Koichi, additional, Nakagawa, Takayuki, additional, Yoshimi, Yuki, additional, Kunimatsu, Ryo, additional, Ito, Shota, additional, Koizumi, Yuma, additional, Ogasawara, Tomohiro, additional, Aikawa, Tomonao, additional, and Tanimoto, Kotaro, additional

Published
2024
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8. Protective Effect of Ergothioneine against Oxidative Stress-Induced Chondrocyte Death.

Author

Sakata, Shuzo, Kunimatsu, Ryo, and Tanimoto, Kotaro

Subjects
AMINO acid derivatives, REACTIVE oxygen species, MEMBRANE transport proteins, LACTATE dehydrogenase, RHEUMATOID arthritis, OXIDATIVE stress, CARTILAGE regeneration
Abstract

Reactive oxygen species (ROS) induce oxidative stress in cells and are associated with various diseases, including autoimmune diseases. Ergothioneine (EGT) is a natural amino acid derivative derived from the ergot fungus and has been reported to exhibit an effective antioxidant function in many models of oxidative stress-related diseases. Recently, mutations in OCTN1, a membrane transporter of EGT, have been reported to be associated with rheumatoid arthritis. Therefore, we investigated the chondrocyte-protective function of EGT using a model of oxidative stress-induced injury of chondrocytes by hydrogen peroxide (H2O2). Human chondrocytes were subjected to oxidative stress induced by H2O2 treatment, and cell viability, the activity of lactate dehydrogenase (LDH) released into the medium, dead cell ratio, intracellular ROS production, and mitochondrial morphology were assessed. EGT improved chondrocyte viability and LDH activity in the medium and strongly suppressed the dead cell ratio. EGT also exerted protective effects on intracellular ROS production and mitochondrial morphology. These results provide evidence to support the protective effects of EGT on chondrocytes induced by oxidative stress. [ABSTRACT FROM AUTHOR]

Published
2024
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16. Comparison of Orthodontic Tooth Movement of Regenerated Bone Induced by Carbonated Hydroxyapatite or Deproteinized Bovine Bone Mineral in Beagle Dogs.

Author

Abe, Takaharu, Kunimatsu, Ryo, and Tanimoto, Kotaro

Subjects
*CORRECTIVE orthodontics, *BEAGLE (Dog breed), *BONE regeneration, *ROOT resorption (Teeth), *HYDROXYAPATITE, *ALVEOLAR process, *MANDIBLE
Abstract

Orthodontic treatments often involve tooth movement to improve dental alignment. In this study, we aimed to compare tooth movement in regenerated bone induced by two different bone fillers, carbonated hydroxyapatite (CAP) and deproteinized bovine bone mineral (DBBM). Four beagle dogs were used in this comparative study. The first, second, and fourth lower mandibular premolars (P1, P2, and P4) on both sides of the mouth were extracted, and CAP was implanted into the extraction site on the left side and DBBM into the right side. Following regenerative bone healing, orthodontic devices were attached to perform orthodontic tooth movement of the lower third mandibular premolar (P3) on both sides. X-ray examination, intraoral scan, and histological analysis were performed. The Mann–Whitney U test was used for statistical analysis, and p < 0.05 was considered significant. Bone regeneration and orthodontic tooth movement were observed in the CAP and DBBM groups. Histologically, normal periodontal tissue remodeling was observed on the compression and tension sides of CAP and DBBM. No statistical difference was observed in the number of osteoclasts around the periodontal ligament and the root resorption area. Orthodontic tooth movement of regenerated bone induced by CAP and DBBM was therefore achieved. [ABSTRACT FROM AUTHOR]

Published
2024
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25. Combination of Carbonate Hydroxyapatite and Stem Cells from Human Deciduous Teeth Promotes Bone Regeneration by Enhancing BMP-2, VEGF and CD31 Expression in Immunodeficient Mice

Author

Putranti, Nurul Aisyah Rizky, primary, Kunimatsu, Ryo, additional, Rikitake, Kodai, additional, Hiraki, Tomoka, additional, Nakajima, Kengo, additional, Abe, Takaharu, additional, Tsuka, Yuji, additional, Sakata, Shuzo, additional, Nakatani, Ayaka, additional, Nikawa, Hiroki, additional, and Tanimoto, Kotaro, additional

Published
2022
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26. Effect of CD146+ SHED on bone regeneration in a mouse calvaria defect model.

Author

Rikitake, Kodai, Kunimatsu, Ryo, Yoshimi, Yuki, Nakajima, Kengo, Hiraki, Tomoka, Aisyah Rizky Putranti, Nurul, Tsuka, Yuji, Abe, Takaharu, Ando, Kazuyo, Hayashi, Yoko, Nikawa, Hiroki, and Tanimoto, Kotaro

Subjects
*TOOTH transplantation, *CELL differentiation, *ENDOTHELIAL cells, *SKULL, *DECIDUOUS teeth, *ANIMAL experimentation, *DENTAL pulp, *RESEARCH funding, *BONE regeneration
Abstract

Objective: Stem cells from human exfoliated deciduous teeth (SHED) have bone regeneration ability and potential therapeutic applications. CD146, a cell adhesion protein expressed by vascular endothelial cells, is involved in osteoblastic differentiation of stem cells. The effect of CD146 on SHED‐mediated bone regeneration in vivo remains unknown. We aimed to establish efficient conditions for SHED transplantation. Materials and methods: SHED were isolated from the pulp of an extracted deciduous tooth and cultured; CD146‐positive (CD146+) and CD146‐negative (CD146−) populations were sorted. Heterogeneous populations of SHED and CD146+ and CD146− cells were transplanted into bone defects generated in the skulls of immunodeficient mice. Micro‐computed tomography was performed immediately and 4 and 8 weeks later. Histological and immunohistochemical assessments were performed 8 weeks later. Results: Bone regeneration was observed upon transplantation with CD146+ and heterogeneous populations of SHED, with significantly higher bone regeneration observed with CD146+ cells. Bone regeneration was higher in the CD146− group than in the control group, but significantly lower than that in the other transplant groups at 4 and 8 weeks. Histological and immunohistochemical assessments revealed that CD146+ cells promoted bone regeneration and angiogenesis. Conclusion: Transplantation of CD146+ SHED into bone defects may be useful for bone regeneration. [ABSTRACT FROM AUTHOR]

Published
2023
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30. Effect of CD146+ SHED on bone regeneration in a mouse calvaria defect model

Author

Rikitake, Kodai, primary, Kunimatsu, Ryo, additional, Yoshimi, Yuki, additional, Nakajima, Kengo, additional, Hiraki, Tomoka, additional, Aisyah Rizky Putranti, Nurul, additional, Tsuka, Yuji, additional, Abe, Takaharu, additional, Ando, Kazuyo, additional, Hayashi, Yoko, additional, Nikawa, Hiroki, additional, and Tanimoto, Kotaro, additional

Published
2021
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31. High-frequency near-infrared diode laser irradiation suppresses IL-1β-induced inflammatory cytokine expression and NF-κB signaling pathways in human primary chondrocytes

Author

Sakata, Shuzo, primary, Kunimatsu, Ryo, additional, Tsuka, Yuji, additional, Nakatani, Ayaka, additional, Gunji, Hidemi, additional, Yanoshita, Makoto, additional, Kado, Isamu, additional, Ito, Shota, additional, Putranti, Nurul Aisyah Rizky, additional, Prasetya, Rendra Chriestedy, additional, Hirose, Naoto, additional, and Tanimoto, Kotaro, additional

Published
2021
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32. Effects of baicalin on the proliferation and expression of OPG and RANKL in human cementoblast-lineage cells

Author

Kunimatsu, Ryo, primary, Kimura, Aya, additional, Sakata, Shuzo, additional, Tsuka, Yuji, additional, Yoshimi, Yuki, additional, Abe, Takaharu, additional, Kado, Isamu, additional, Yashima, Yuka, additional, Izumino, Jin, additional, Nakatani, Ayaka, additional, Kitagawa, Masae, additional, Miyauchi, Mutsumi, additional, Takata, Takashi, additional, and Tanimoto, Kotaro, additional

Published
2021
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36. Effect of CD146 on Bone Regeneration by Transplantation of Stem Cells from Human Exfoliated Deciduous Teeth into Mouse Skull Defect

Author

Rikitake, Kodai, primary, Kunimatsu, Ryo, additional, Yoshimi, Yuki, additional, Nakajima, Kengo, additional, Hiraki, Tomoka, additional, Putranti, Nurul Aisyah Rizky, additional, Tsuka, Yuji, additional, Abe, Takaharu, additional, Ando, Kazuyo, additional, Hayashi, Yoko, additional, Nikawa, Hiroki, additional, and Tanimoto, Kotaro, additional

Published
2021
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37. FAK inhibition protects condylar cartilage under excessive mechanical stress

Author

Azusa Onishi, Eri Tsuboi, Kotaro Tanimoto, Mami Takano, Sayuri Nishiyama, Chikako Sumi, Makoto Yanoshita, Yuka Yamauchi, Kunimatsu Ryo, Naoto Hirose, and Yuki Asakawa

Subjects
Cartilage, Articular, Pathology, medicine.medical_specialty, H&E stain, Inflammation, Osteoarthritis, Organ culture, Proinflammatory cytokine, Focal adhesion, 03 medical and health sciences, 0302 clinical medicine, Chondrocytes, medicine, Animals, General Dentistry, Temporomandibular Joint, Chemistry, Cartilage, 030206 dentistry, Temporomandibular Joint Disorders, medicine.disease, Rats, medicine.anatomical_structure, Otorhinolaryngology, 030220 oncology & carcinogenesis, Focal Adhesion Protein-Tyrosine Kinases, Stress, Mechanical, Signal transduction, medicine.symptom
Abstract

Objectives Excessive mechanical stress is assumed to be a major cause of temporomandibular joint (TMJ) osteoarthritis (OA). +Focal adhesion kinase (FAK) is a cytoplasmic non-receptor tyrosine kinase involved in a variety of signaling pathways. Little has been reported on the function of FAK in TMJ-OA. In the present study, we investigated the effect of FAK inhibition on TMJ cartilage under excessive mechanical loading stress. Materials and methods Articular cartilage explants were harvested from the TMJ of rats and subjected to mechanical loading in the presence of an FAK inhibitor in organ culture. The gene expression of inflammatory cytokines was examined after the application of mechanical loading with or without FAK inhibitor. Paraffin-embedded sections of articular cartilage were stained with hematoxylin and eosin, safranin O and fast Green, toluidine blue, TUNEL staining, and immunohistochemical staining and was performed to investigate the protein expression of IL-1β and MMP-13. Results Treatment with FAK inhibitor reduced the gene expression of inflammatory cytokines and inhibited the degradation of articular cartilage, as determined histologically. FAK inhibitor treatment also suppressed the protein expression of IL-1β and MMP-13 in the hypertrophic zone, as determined immunohistologically. Conclusion Treatment with FAK inhibitor suppresses inflammation and protects condylar cartilage under excessive mechanical loading.

Published
2020

38. High-frequency Near-infrared Diode Laser Irradiation Attenuates IL-1β-induced Expression of Inflammatory Cytokines and Matrix Metalloproteinases in Human Primary Chondrocytes

Author

Sakata, Shuzo, Kunimatsu, Ryo, Tsuka, Yuji, Nakatani, Ayaka, Hiraki, Tomoka, Gunji, Hidemi, Hirose, Naoto, Yanoshita, Makoto, Tanimoto, Kotaro, and Putranti, Nurul Aisyah Rizky

Subjects
medicinal_chemistry
Abstract

High-frequency near-infrared diode laser provides high peak output, low heat accumulation, and efficient biostimulation. Although these characteristics are considered suitable for osteoarthritis (OA) treatment, the effect of high-frequency near-infrared diode laser in in vitro or in vivo OA models has not yet been reported. Therefore, we aimed to assess the biological effects of high-frequency near-infrared diode laser irradiation on IL-1β-induced chondrocyte inflammation in an in vitro OA model. Normal Human Articular Chondrocyte-Knee (NHAC-Kn) cells were stimulated with human recombinant IL-1β and irradiated with high-frequency near-infrared diode laser (910 nm, 4 or 8 J/cm2). The mRNA and protein expression of relevant inflammation- and cartilage destruction-related proteins was analyzed. IL-1β treatment significantly increased the mRNA levels of IL-1β, IL-6, TNF-α, MMP-1, MMP-3, and MMP-13. High-frequency near-infrared diode laser irradiation significantly reduced the IL-1β-induced expression of IL-1β, IL-6, TNF-α, MMP-1, and MMP-3. Similarly, high-frequency near-infrared diode laser irradiation decreased the IL-1β-induced increase in protein expression and secreted levels of MMP-1 and MMP-3. These results highlight the therapeutic potential of high-frequency near-infrared diode laser in OA.

Published
2020

41. Masticatory Function Assessment of Adult Patients With Cleft Lip and Palate After Orthodontic Treatment.

Author

Miura, Sayumi, Ueda, Hiroshi, Iwai, Koji, Concepcion Medina, Cynthia, Ishida, Eri, Kunimatsu, Ryo, and Tanimoto, Kotaro

Subjects
KRUSKAL-Wallis Test, ACADEMIC medical centers, TEMPOROMANDIBULAR joint, MASTICATORY muscles, CLEFT palate, ORTHODONTICS, MASSETER muscle, CLEFT lip, DENTAL occlusion, MALOCCLUSION, MASTICATION, DESCRIPTIVE statistics, ELECTROMYOGRAPHY, ADULTS
Abstract

Objective: To determine whether orthodontically treated patients with cleft lip and palate (CLP) possess a different masticatory function than those of untreated patients with normal occlusion. Design: Occlusal contact area, occlusal force, as well as masseter and anterior temporal muscular activity were measured during maximum voluntary clenching (MVC) tests. Mandibular left and right lateral movements during mastication were also assessed. To further elucidate the nature of masticatory function, especially to determine the rate of abnormal jaw movement patterns, a parametric error index (EI) was set. Finally, masticatory efficiency was evaluated with a glucose sensitive measuring device. Participants: Fifteen patients with CLP who had previously completed the orthodontic treatments required to achieve an acceptable and more harmonious occlusion accepted to volunteer in this study along with 21 untreated patients who already possessed a normal occlusion. Results: Patients with CLP showed a significantly lower occlusal force, reduced occlusal contact area, and decreased masticatory efficiency as well as significantly higher EI value when compared with controls. However, there was no significant difference when analyzing muscle activity, although masticatory efficiency was significantly different between the 2 groups. Despite this result, the scores obtained by the patients with CLP in the masticatory efficiency tests were still in the normal range. Conclusions: Orthodontic treatment for adult patients with CLP provides a satisfactory result for the patients' masticatory ability albeit significantly less ideal compared with untreated patients with normal occlusion. [ABSTRACT FROM AUTHOR]

Published
2022
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43. Baicalin inhibits root resorption during tooth movement in a rodent model

Author

Kunimatsu, Ryo, primary, Kimura, Aya, additional, Tsuka, Yuji, additional, Horie, Kayo, additional, Yoshimi, Yuki, additional, Awada, Tetsuya, additional, Gunji, Hidemi, additional, Abe, Takaharu, additional, Nakajima, Kengo, additional, Sakata, Syuzou, additional, Nakatani, Ayaka, additional, and Tanimoto, Kotaro, additional

Published
2020
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44. High-Frequency Near-Infrared Diode Laser Irradiation Attenuates IL-1β-Induced Expression of Inflammatory Cytokines and Matrix Metalloproteinases in Human Primary Chondrocytes

Author

Sakata, Shuzo, primary, Kunimatsu, Ryo, additional, Tsuka, Yuji, additional, Nakatani, Ayaka, additional, Hiraki, Tomoka, additional, Gunji, Hidemi, additional, Hirose, Naoto, additional, Yanoshita, Makoto, additional, Putranti, Nurul Aisyah Rizky, additional, and Tanimoto, Kotaro, additional

Published
2020
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50. Baicalin Promotes Osteogenic Differentiation of Human Cementoblast Lineage Cells Via the Wnt/β Catenin Signaling Pathway

Author

Kimura, Aya, primary, Kunimatsu, Ryo, additional, Yoshimi, Yuki, additional, Tsuka, Yuji, additional, Awada, Tetsuya, additional, Horie, Kayo, additional, Gunji, Hidemi, additional, Abe, Takaharu, additional, Nakajima, Kengo, additional, Kitagawa, Masae, additional, Miyauchi, Mutsumi, additional, Takata, Takashi, additional, and Tanimoto, Kotaro, additional

Published
2019
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