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188 results on '"Kunert O"'

2. An UHPLC-HRMS-based metabolite profiling approach to detect potentially anti-inflammatory active compounds in Chinese Lonicera species

Author

Pferschy-Wenzig, E M, additional, Ortmann, S, additional, Atanasov, A G, additional, Hellauer, K, additional, Hartler, J, additional, Kunert, O, additional, Gold-Binder, M, additional, Ladurner, A, additional, Heiß, E H, additional, Latkolik, S, additional, Zhao, Y M, additional, Raab, P, additional, Monschein, M, additional, Trummer, N, additional, Samuel, B, additional, Crockett, S, additional, Miao, J H, additional, Thallinger, G G, additional, Dirsch, V M, additional, and Bauer, R, additional

Published
2021
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16. Measurement of fractional whole-body gluconeogenesis in humans from blood samples using ²H nuclear magnetic resonance spectroscopy

Author

Kunert, O., Stingl, H., Rosian, E., Krssak, M., Bernroider, E., Seebacher, W., Zangger, K., Staehr, P., Chandramouli, V., Landau, B.R., Nowotny, P., Waldhausl, W., Haslinger, E., and Roden, M.

Subjects
Gluconeogenesis -- Physiological aspects -- Measurement -- Usage, Nuclear magnetic resonance spectroscopy -- Usage -- Measurement -- Physiological aspects, Type 2 diabetes -- Development and progression, Health, Usage, Physiological aspects, Development and progression, Measurement
Abstract

Several problems limit quantification of gluconeogenesis. We applied in vitro ²H-nuclear magnetic resonance (NMR) spectroscopy to simultaneously measure ²H in all glucose carbons for direct assessment of gluconeogenesis. This method was compared with ²H measurement in carbons 5 and 2 using gas chromatography-mass spectrometry (hexamethylenetetramine [HMT]) and with in vivo [sup.13]C magnetic resonance spectroscopy (MRS). After 14 h of fasting, and following ²[H.sub.2]O ingestion, blood was obtained from nine healthy and seven type 2 diabetic subjects. Glucose was purified, acetylated, and analyzed for ²H in carbons 1-6 with ²H-NMR. Using 5:2 ratios, gluconeogenesis increased (P < 0.05) over time and mean gluconeogenesis was lower in control subjects than in type 2 diabetic patients (63 ± 3 vs. 75 ± 2%, P < 0.01). [sup.13]C-MRS revealed higher hepatic glycogenolysis in control subjects (3.9 ± 0.4 vs. 2.3 ± 0.2 µmol * [kg.sup.-1] * [min.sup.-1]) yielding mean contribution of gluconeogenesis of 65 ± 3 and 77 ± 2% (P < 0.005). Measurement of gluconeogenesis by ²H-NMR correlated linearly with [sup.13]C-MRS (r = 0.758, P = 0.0007) and HMT (r = 0.759, P = 0.0007). In an additional protocol, ²H enrichments demonstrated a fast decline of gluconeogenesis from ~100 to ~68% (P < 0.02) within 4 h of galactose infusion after 40-44 h of fasting. Thus, in vitro ²H-NMR offers an alternative approach to determine fractional gluconeogenesis in good agreement with standard methods and allows monitoring of rapid metabolic alterations. Diabetes 52:2475-2482, 2003, In type 2 diabetic patients, gluconeogenesis primarily accounts for increased endogenous glucose production (EGP) and fasting plasma glucose concentrations (1). Previous methods for measuring gluconeogenesis yielded a wide range of [...]

Published
2003

18. Phenolic compounds of Iris adriatica and their antimycobacterial effects

Author

Ochensberger Sandra, Alperth Fabian, Mitić Božena, Kunert Olaf, Mayer Stefanie, Mourão Maria Ferreira, Turek Ivana, Luca Simon Vlad, Skalicka-Woźniak Krystyna, Maleš Željan, Hruševar Dario, Duka Ivan, and Bucar Franz

Subjects
iris adriatica, radix iridis, phenolic compounds, mycobacterium smegmatis, resistance modulation, ethidium bromide, mic, Pharmaceutical industry, HD9665-9675
Abstract

Little is known about the pharmacological activities of Iris adriatica (Iridaceae), a plant endemic to Dalmatia (Croatia). We therefore performed a bioassay-guided fractionation including high-performance counter current chromatography (HPCCC) and antibacterial tests using Mycobacterium smegmatis mc2 155. One obtained fraction was found to be antimycobacterially active with a MIC of 64 mg L−1. Furthermore, fractions were tested for resistance modulatory effects using ethidium bromide as substrate. We were able to identify the pure isoflavonic compounds irigenin and irilone and a fraction containing mainly benzophenone 2,4,6-trihydroxy-4-methoxy-benzophenone, responsible for the resistance-modulatory activity of this plant.

Published
2019
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