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187 results on '"Kunert, O."'

2. An UHPLC-HRMS-based metabolite profiling approach to detect potentially anti-inflammatory active compounds in Chinese Lonicera species

Author

Pferschy-Wenzig, E M, additional, Ortmann, S, additional, Atanasov, A G, additional, Hellauer, K, additional, Hartler, J, additional, Kunert, O, additional, Gold-Binder, M, additional, Ladurner, A, additional, Heiß, E H, additional, Latkolik, S, additional, Zhao, Y M, additional, Raab, P, additional, Monschein, M, additional, Trummer, N, additional, Samuel, B, additional, Crockett, S, additional, Miao, J H, additional, Thallinger, G G, additional, Dirsch, V M, additional, and Bauer, R, additional

Published
2021
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16. Measurement of fractional whole-body gluconeogenesis in humans from blood samples using ²H nuclear magnetic resonance spectroscopy

Author

Kunert, O., Stingl, H., Rosian, E., Krssak, M., Bernroider, E., Seebacher, W., Zangger, K., Staehr, P., Chandramouli, V., Landau, B.R., Nowotny, P., Waldhausl, W., Haslinger, E., and Roden, M.

Subjects
Gluconeogenesis -- Physiological aspects -- Measurement -- Usage, Nuclear magnetic resonance spectroscopy -- Usage -- Measurement -- Physiological aspects, Type 2 diabetes -- Development and progression, Health, Usage, Physiological aspects, Development and progression, Measurement
Abstract

Several problems limit quantification of gluconeogenesis. We applied in vitro ²H-nuclear magnetic resonance (NMR) spectroscopy to simultaneously measure ²H in all glucose carbons for direct assessment of gluconeogenesis. This method was compared with ²H measurement in carbons 5 and 2 using gas chromatography-mass spectrometry (hexamethylenetetramine [HMT]) and with in vivo [sup.13]C magnetic resonance spectroscopy (MRS). After 14 h of fasting, and following ²[H.sub.2]O ingestion, blood was obtained from nine healthy and seven type 2 diabetic subjects. Glucose was purified, acetylated, and analyzed for ²H in carbons 1-6 with ²H-NMR. Using 5:2 ratios, gluconeogenesis increased (P < 0.05) over time and mean gluconeogenesis was lower in control subjects than in type 2 diabetic patients (63 ± 3 vs. 75 ± 2%, P < 0.01). [sup.13]C-MRS revealed higher hepatic glycogenolysis in control subjects (3.9 ± 0.4 vs. 2.3 ± 0.2 µmol * [kg.sup.-1] * [min.sup.-1]) yielding mean contribution of gluconeogenesis of 65 ± 3 and 77 ± 2% (P < 0.005). Measurement of gluconeogenesis by ²H-NMR correlated linearly with [sup.13]C-MRS (r = 0.758, P = 0.0007) and HMT (r = 0.759, P = 0.0007). In an additional protocol, ²H enrichments demonstrated a fast decline of gluconeogenesis from ~100 to ~68% (P < 0.02) within 4 h of galactose infusion after 40-44 h of fasting. Thus, in vitro ²H-NMR offers an alternative approach to determine fractional gluconeogenesis in good agreement with standard methods and allows monitoring of rapid metabolic alterations. Diabetes 52:2475-2482, 2003, In type 2 diabetic patients, gluconeogenesis primarily accounts for increased endogenous glucose production (EGP) and fasting plasma glucose concentrations (1). Previous methods for measuring gluconeogenesis yielded a wide range of [...]

Published
2003

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