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9. Comprehensive study of hormone-independent highly productive strain of Rauvolfia serpentina tissue culture as a source of indole alkaloids.

Author

Kunakh, V. A., Konvalyuk, I. I., Mozhylevska, L. P., Bieda, O. A., Twardovska, M. O., Andreev, I. O., and Yarmolyuk, S. M.

Subjects
*INDOLE alkaloids, *TISSUE culture, *PLANT tissue culture, *YOHIMBINE, *BIOMASS, *ALKALOIDS
Abstract

Aim. To characterize a new hormone-independent strain 1-27 of R. serpentina tissue culture. Methods. Plant tissue culture, biochemical and cytological analysis, statistical methods. Results. The strain is maintained on the specially designed simple-composition hormone-free 10C medium. The maximum biomass yield was at day 69 of subculture (693.5 g/l of live biomass, 43.0 g/l of dry biomass), the weight of cell biomass increased 15-18 times over a subculture period. The maximum content of indole alkaloids was observed from 88 to 108 days of subculture. The dry biomass contained 4.0% of total indole alkaloids, 1.64% of ajmaline-like alkaloids, 0.789% of ajmaline, 0.337% of vomilenine, and 0.006% of each yohimbine and reserpine. The strain is a heterogeneous mixoploid cell population with a modal class of 22 to 33 chromosomes (2n = 22), which characterized by significant cell and nuclear polymorphism. Conclusions. The K-27M strain of R. serpentina cultured tissue is a prospective producer of indole alkaloids. [ABSTRACT FROM AUTHOR]

Published
2023
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33. First record of the endophytic bacteria of Deschampsia antarctica Ė. Desv. from two distant localities of the maritime Antarctic

Author

Podolich, O. (Olga), Prekrasna, I. (Ievgeniia), Parnikoza, I. (Ivan), Voznyuk, T. (Tamara), Zubova, G. (Ganna), Zaets, I. (Iryna), Miryuta, N. (Natalia), Myryuta, G. (Ganna), Poronnik, O. (Oksana), Kozeretska, I. (Iryna), Kunakh, V. (Viktor), Pirttilä, A. M. (Anna Maria), Dykyi, E. (Evgen), Kozyrovska, N. (Natalia), Podolich, O. (Olga), Prekrasna, I. (Ievgeniia), Parnikoza, I. (Ivan), Voznyuk, T. (Tamara), Zubova, G. (Ganna), Zaets, I. (Iryna), Miryuta, N. (Natalia), Myryuta, G. (Ganna), Poronnik, O. (Oksana), Kozeretska, I. (Iryna), Kunakh, V. (Viktor), Pirttilä, A. M. (Anna Maria), Dykyi, E. (Evgen), and Kozyrovska, N. (Natalia)

Abstract

Endophytic bacteria, recognized for their beneficial effects on plant development and adaptation, can facilitate the survival of Antarctic plants in severe environments. Here we studied endophytes of the vascular plant Deschampsia antarctica Ė. Desv. from two distantly located regions in the maritime Antarctic: King George Island (South Shetland Islands) and Galindez Island (Argentine Islands). Bacterial group-specific PCR indicated presence of Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Firmicutes, Cytophaga-Flavobacteria and Actinobacteria in root and leaf endosphere of D. antarctica sampled at four distinct sites of both locations. The diversity of endophytic bacteria was significantly higher in the leaves compared to the roots in plants from Galindez Island. Similarly, the diversity of endophytes was higher in the leaves rather than roots of plants from the King George Island. Twelve bacterial species were isolated from roots of D. antarctica of Galindez Island (the Karpaty Ridge and the Meteo Point) and identified by sequencing the 16S rRNA gene. Isolates were dominated by the Pseudomonas genus, followed by the genera Bacillus and Micrococcus. The vast majority of the isolates exhibited cellulase and pectinase activities, however, Bacillus spp. expressed neither of them, suggesting lack of genetic flow of these traits in endophytic bacilli in the maritime Antarctic. Pseudomonas sp. IMBG305 promoted an increase in the leaf number in most of the treated plant genotypes when compared with non-inoculated plants, and a rapid vegetation period of D. antarctica cultured in vitro, albeit the length of leaves in the treated plants was significantly lower, and flavonoid content leveled off in all treated plants. D. antarctica is known to develop diverse ecotypes with regard to ecological conditions, such as organic input, moisture or wind exposition. The D. antarctica phenotype could be extended further through the endophyte colonization, since

Published
2021

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