1. Human Cyclophilin B forms part of a multi-protein complex during erythrocyte invasion by Plasmodium falciparum.
- Author
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Prakash P, Zeeshan M, Saini E, Muneer A, Khurana S, Kumar Chourasia B, Deshmukh A, Kaur I, Dabral S, Singh N, Anam Z, Chaurasiya A, Kaushik S, Dahiya P, Kalamuddin M, Kumar Thakur J, Mohmmed A, Ranganathan A, and Malhotra P
- Subjects
- Animals, Basigin metabolism, Carrier Proteins metabolism, Erythrocytes parasitology, Female, Host-Parasite Interactions, Humans, Merozoites metabolism, Merozoites physiology, Mice, Inbred BALB C, Plasmodium falciparum physiology, Protein Binding, Rabbits, Cyclophilins metabolism, Erythrocytes metabolism, Plasmodium falciparum metabolism, Protozoan Proteins metabolism
- Abstract
Invasion of human erythrocytes by Plasmodium falciparum merozoites involves multiple interactions between host receptors and their merozoite ligands. Here we report human Cyclophilin B as a receptor for PfRhopH3 during merozoite invasion. Localization and binding studies show that Cyclophilin B is present on the erythrocytes and binds strongly to merozoites. We demonstrate that PfRhopH3 binds to the RBCs and their treatment with Cyclosporin A prevents merozoite invasion. We also show a multi-protein complex involving Cyclophilin B and Basigin, as well as PfRhopH3 and PfRh5 that aids the invasion. Furthermore, we report identification of a de novo peptide CDP3 that binds Cyclophilin B and blocks invasion by up to 80%. Collectively, our data provide evidence of compounded interactions between host receptors and merozoite surface proteins and paves the way for developing peptide and small-molecules that inhibit the protein-protein interactions, individually or in toto, leading to abrogation of the invasion process.
- Published
- 2017
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