The impact of electrical stimulation (ES) on growth and regeneration of nerves has been widely studied, with field strength, type of ES, and duration of application all demonstrating impact on the regeneration. However, little information exists directly comparing the frequency of alternating current (AC) stimulation impacts these cell behaviors. This study investigated the biological behavior of chick dorsal root ganglia (DRG): growth of the neurites, spreading of cells from DRG body, cell viability, neurite density and neurite directionality, at 4 frequencies to determine the most efficient frequency with the healthiest neurite growth. E9 chick DRG were stimulated directly with platinum electrodes with AC sinusoidal signals of 2.5Vp-p amplitude and varying frequencies of 20Hz, 200Hz, 1MHz and 20MHz. Characterization of DRG was done using antibody staining. From this study, it can be concluded that application of AC EF increased the length, density and directionality of neurites at low frequencies. Also, application of ES increased cell spreading from the DRG body, which may guide the neurites. Moreover, neurites have greater growth in the high amplitude region of the stimulation chamber (or closer to the anode), indicating that the growth and density is influenced by the voltage intensity. From literature survey, the changes in the biological characteristics of DRG neurites may be due to changes in DRG conductivity.